NSFL1C Antibody Cat. No.: 22-399

NSFL1C Antibody

Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (22-399). Immunofluorescence analysis of HeLa cells using NSFL1C antibody (22-399). Western blot was performed from Blue: DAPI for nuclear staining. the immunoprecipitate using NSFL1C antibody (22-399) at a dilition of 1:1000.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion containing a sequence corresponding to amino acids 1-185 of IMMUNOGEN: human NSFL1C (NP_057227.2).

TESTED APPLICATIONS: IF, IP, WB

September 28, 2021 1 https://www.prosci-inc.com/nsfl1c-antibody-22-399.html WB: ,1:500 - 1:2000

APPLICATIONS: IF: ,1:50 - 1:200

IP: ,1:50 - 1:100

POSITIVE CONTROL: 1) 293T

2) U-251MG

3) Mouse kidney

4) Mouse brain

5) Rat brain

6) Rat kidney

PREDICTED MOLECULAR Observed: 41kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: NSFL1C

P47, UBX1, UBXD1UBXN2C, dJ776F14.1, NSFL1 cofactor p47, NSFL1 (p97) cofactor (p47), ALTERNATE NAMES: SHP1 homolog, UBX domain-containing protein 2C, p97 cofactor p47

GENE ID: 55968

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A BACKGROUND: trimer of the protein encoded by this binds a hexamer of cytosolic p97 and is required for p97- mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on 8.

September 28, 2021 2 https://www.prosci-inc.com/nsfl1c-antibody-22-399.html ANTIBODIES FOR RESEARCH USE ONLY.

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September 28, 2021 3 https://www.prosci-inc.com/nsfl1c-antibody-22-399.html