NSFL1C Antibody Cat. No.: 22-399 NSFL1C Antibody Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (22-399). Immunofluorescence analysis of HeLa cells using NSFL1C antibody (22-399). Western blot was performed from Blue: DAPI for nuclear staining. the immunoprecipitate using NSFL1C antibody (22-399) at a dilition of 1:1000. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of IMMUNOGEN: human NSFL1C (NP_057227.2). TESTED APPLICATIONS: IF, IP, WB September 28, 2021 1 https://www.prosci-inc.com/nsfl1c-antibody-22-399.html WB: ,1:500 - 1:2000 APPLICATIONS: IF: ,1:50 - 1:200 IP: ,1:50 - 1:100 POSITIVE CONTROL: 1) 293T 2) U-251MG 3) Mouse kidney 4) Mouse brain 5) Rat brain 6) Rat kidney PREDICTED MOLECULAR Observed: 41kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: NSFL1C P47, UBX1, UBXD1UBXN2C, dJ776F14.1, NSFL1 cofactor p47, NSFL1 (p97) cofactor (p47), ALTERNATE NAMES: SHP1 homolog, UBX domain-containing protein 2C, p97 cofactor p47 GENE ID: 55968 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A BACKGROUND: trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97- mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. September 28, 2021 2 https://www.prosci-inc.com/nsfl1c-antibody-22-399.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 3 https://www.prosci-inc.com/nsfl1c-antibody-22-399.html.