DOCSLIB.ORG

Role of PCK1 Gene on Oil Tea-Induced Glucose Homeostasis and Type 2

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Role of PCK1 Gene on Oil Tea-Induced Glucose Homeostasis and Type 2 Hu et al. Nutrition & Metabolism (2019) 16:12 https://doi.org/10.1186/s12986-019-0337-8 RESEARCH Open Access Role of PCK1 gene on oil tea-induced glucose homeostasis and type 2 diabetes: an animal experiment and a case-control study Qiantu Hu1†, Huafeng Chen2†, Yanli Zuo3†, Qin He2, Xuan He2, Steve Simpson Jr4,5, Wei Huang2, Hui Yang2, Haiying Zhang1,6* and Rui Lin1,2,6* Abstract Background: Oil tea is a type of traditional tea beverage used for treating various ailments in minority population in Guangxi, China. Our previous study showed oil tea improved glucose and lipid levels in type 2 diabetic mice. Yet, the underling molecular mechanisms are still not understood. This study aimed at assessing the effect of oil tea on glucose homeostasis and elucidating the molecular mechanisms underlying the oil tea-induced antidiabetic effects. Methods: Twenty seven db/db mice were gavaged with saline, metformin and oil tea for 8 weeks with measurement of biochemical profiles. A real-time2 (RT2) profiler polymerase chain reaction (PCR) array comprising 84 genes involved in glucose metabolism was measured and validated by quantitative PCR (qPCR). The association between the candidate genes and type 2 diabetes were further analyzed in a case-control study in the Chinese minority population. Results: Oil tea treatment facilitated glucose homeostasis by decreasing fasting blood glucose and total cholesterol, and improving glucose tolerance. Suppressing phosphoenolpyruvate carboxykinase 1 (PCK1) expression was observed in the oil tea treatment group and the expression was significantly correlated with fasting blood glucose levels. Target prediction and functional annotation by WEB-based GEne SeT AnaLysis Toolkit (WebGestalt) revealed that PCK1 mainly involved in the glycolysis/gluconeogenesis pathway among the top Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways. Both rs707555 and rs2071023 in PCK1 were significantly associated with type 2 diabetes in the minority population of Guangxi. Conclusion: Our findings indicated oil tea improved glucose homeostasis via down-regulation of PCK1 and PCK1 may be a genetic marker for the treatment of type 2 diabetes. Keywords: Fasting blood glucose, Glucose tolerance, Glycolysis/gluconeogenesis pathway, Oil tea, PCK1,RT2 profiler PCR array, SNP, Type 2 diabetes * Correspondence: [email protected]; [email protected] †Qiantu Hu, Huafeng Chen and Yanli Zuo contributed equally to this work. 1Center for Genomic and Personalized Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China Full list of author information is available at the end of the article © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Hu et al. Nutrition & Metabolism (2019) 16:12 Page 2 of 12 Background added, and the mixture boiled for 1 min and the result- Oil tea is a unique beverage and an important dietary ing decoction retained. This process was repeated twice, component in the minority areas of Guangxi Province, whereupon the mixed decoction was called oil tea. The China. Oil tea is rich in polyphenol, caffeine, 6-gingerol oil tea decoction then was concentrated by a rotary and several minerals [1], and is the most popular vacuum evaporator (IKA Works, Germany) and stored traditional beverage used for treating various ailments in at − 80 °C until use. Guangxi [2]. Green tea and ginger, as the main ingredi- ents of oil tea, individually have exhibited various bio- Animal model and experimental design logical activities, including anticancer effects, antioxidative The experimental procedures were approved by the effects, and antidiabetic effects [3–5]. Previously, our Animal Ethics Committee of Guangxi Center for Disease study showed oil tea treatment improved hyperglycemia, Prevention and Control (Approval No. 20160003). Six glucose intolerance and hyperlipidemia in db/db mice [1]. week-old male diabetic C57BL/KsJ-db/db (db/db) mice While the underlying molecular mechanisms of the were randomly divided assigned to three groups and beneficial effects of oil tea was unknown. orally gavaged with oil tea (4 g/kg body weight (BW)/ Phosphoenolpyruvate carboxykinase 1 (PCK1) is a key day, n = 10), distilled water (0.02 ml/g BW/day, n =7) gluconeogenic enzyme in the liver [6]. It is the cytosolic and metformin (MET, Sigma, USA, the first-line medica- form of phosphoenolpyruvate carboxykinase (PCK or tion for treating T2D, 150 mg/kg BW/day, n =10)for8 PEPCK) and catalyzes the initial step in hepatic gluco- weeks. The oil tea contained 24.6% polyphenols and 0.3% neogenesis. The expression of PCK1 gene is regulated by [6]-gingerol, as described previously [1]. Up to five mice insulin, glucocorticoids, glucagon, cyclic adenosine per cage were maintained in a temperature-controlled monophosphate (cAMP) and diet, all of which have been (22 ± 2 °C) mouse facility on a reverse 12-h light/dark considered associated with diabetic conditions. It has cycle and provided water and chow-diet ad libitum. been reported that PCK1 is up-regulated in diabetic The full nutritional composition of oil tea has been rodent models [7], and overexpression in the transgenic described previously [1]. Body weight and food intake mice resulted in insulin resistance [8] and hyperglycemia were measured each week. [9]. Reciprocally, silencing of PCK1 has been demon- strated to improve glycemia control, insulin sensitivity FBG detection and OGTT test and dyslipidemia in db/db mice [10]. Furthermore, re- Fasting blood glucose (FBG) levels were examined every search has indicated that induced expression of PCK1 week after the db/db mice overnight fasting. The oral may be a useful indicator for effect assessment in dia- glucose tolerance test (OGTT) was performed at weeks betes therapy. Indeed, studies of the effect of Rosmarinic 4 and 8 after the mice were orally gavaged with oil tea acid, insulin, retinoic acid and phenobarbital showed or MET, combined with glucose solution (1 g/kg BW). decreased expression of PCK1 and improvement of Blood glucose levels were measured in tail blood hyperglycemia in diabetic rats [11–13]. Moreover, PCK1 samples collected at 0, 15, 30, 60, 90 and 120 min after has been demonstrated to be a candidate genetic marker glucose treatment. Blood glucose was measured using a for diabetes and obesity risk [14], and more recent glucometer (OMRON Healthcare Co., Ltd., China). research implicated this locus in type 2 diabetes (T2D) risk in Chinese [15], South Asian [16] and Finnish popu- Serum lipid measurement lations [17]. At the end of experiment, mice were killed by cervical In the present study, we investigated the molecular dislocation, and blood samples were collected for the mechanisms of antidiabetic effect of oil tea by examining measurement of biochemical profiles. Levels of serum the expression of PCK1, as well as other genes involved triglycerides (TG), total cholesterol (TC), low-density in glucose metabolism in db/db mice. We also assessed lipoprotein (LDL) and high-density lipoprotein (HDL) whether PCK1 is a genetic marker for T2D risk in the were measured using commercial kits (Shanghai minority population in Guangxi. Enzyme-linked Biotechnology Co., Ltd., China). Insulin was measured using a commercial Mouse Insulin ELISA Methods kit (ALPCO Diagnostics, USA). Preparation of oil tea The oil tea was prepared as previously described [1, 18]. RT2 profiler PCR array analysis In brief, the proportions of green tea, ginger, peanut, After mice were killed, the fresh livers were collected cooking oil and salt were 9:10:5:2:1. The manufacturing and snap frozen in liquid nitrogen, and then stored at − process was as follows: firstly, soaked green tea leaf was 80 °C until analysis. Total RNA was isolated from the stir-fried and beaten with ginger, peanut and cooking oil liver samples of db/db control group and oil tea group in a hot iron pot for 3 min, then hot water and salt using Qiagen RNeasy® Mini Kit (QIAGEN, Shanghai, Hu et al. Nutrition & Metabolism (2019) 16:12 Page 3 of 12 China) according to the manufacturer’s instructions. according to the manufacturer’s instructions. The quan- Single-strand cDNA was synthesized from 1 μg of total titative expressions of genes, again, were calculated from −ΔΔ RNA by reverse transcription reaction using Qiagen RT2 the CT value using the 2 CT method. Those genes First Strand Kit (QIAGEN, Shanghai, China). The cDNA with expressions less than 0.75-fold were selected fur- was mixed with Qiagen PCR RT2 SYBR Green Master ther for pathway and biological process analysis by using Mix (QIAGEN, Shanghai, China). WEB-based GEne SeT AnaLysis Toolkit (WebGestalt, To explore the underlying mechanisms of oil tea http://www.webgestalt.org/). Target genes were those ex- induced-effects, the expression of 84 genes involved in pression significantly differed between the oil tea group glucose metabolism including PCK1 were examined and db/db control group (p < 0.05) and were analyzed using a Qiagen mouse glucose metabolism RT2 profiler the correlation with FBG further. PCR array (PAMM-006Z, QIAGEN, Shanghai, China). Relative quantification of mRNA levels was determined Selection of the human subjects by real-time quantitative PCR using a Bio-Rad CFX96 Eighty-six T2D patients and 286 healthy subjects were Sequence Detector instrument. The quantitative expres- recruited from the nutrition survey conducted in the mi- sion of gene was calculated from the cycle threshold nority areas in Gongcheng Yao Autonomous County and (CT) value of each sample in the linear part of the curve Binyang County, Guangxi, China from 2014 to 2015.
Load more

Recommended publications
  • Resveratrol Improves Glycemic Control in Insulin-Treated Diabetic Rats
    Yonamine et al. Nutrition & Metabolism (2016) 13:44 DOI 10.1186/s12986-016-0103-0 RESEARCH Open Access Resveratrol improves glycemic control in insulin-treated diabetic rats: participation of the hepatic territory Caio Yogi Yonamine1†, Erika Pinheiro-Machado1†, Maria Luiza Michalani1, Helayne Soares Freitas1, Maristela Mitiko Okamoto1, Maria Lucia Corrêa-Giannella2 and Ubiratan Fabres Machado1* Abstract Background: Resveratrol is a natural polyphenol that has been proposed to improve glycemic control in diabetes, by mechanisms that involve improvement in insulin secretion and activity. In type 1 diabetes (T1D), in which insulin therapy is obligatory, resveratrol treatment has never been investigated. The present study aimed to evaluate resveratrol as an adjunctive agent to insulin therapy in a T1D-like experimental model. Methods: Rats were rendered diabetic by streptozotocin (STZ) treatment. Twenty days later, four groups of animals were studied: non-diabetic (ND); diabetic treated with placebo (DP); diabetic treated with insulin (DI) and diabetic treated with insulin plus resveratrol (DIR). After 30 days of treatment, 24-hour urine was collected; then, blood, soleus muscle, proximal small intestine, renal cortex and liver were sampled. Specific glucose transporter proteins were analyzed (Western blotting) in each territory of interest. Solute carrier family 2 member 2 (Slc2a2), phosphoenolpyruvate carboxykinase (Pck1) and glucose-6-phosphatase catalytic subunit (G6pc) mRNAs (qPCR), glycogen storage and sirtuin 1 (SIRT1) activity were analyzed in liver. Results: Diabetes induction increased blood glucose, plasma fructosamine concentrations, and glycosuria. Insulin therapy partially recovered the glycemic control; however, resveratrol as adjunctive therapy additionally improved glycemic control and restored plasma fructosamine concentration to values of non-diabetic rats.
    [Show full text]
  • Protein Interactions in the Cancer Proteome† Cite This: Mol
    Molecular BioSystems View Article Online PAPER View Journal | View Issue Small-molecule binding sites to explore protein– protein interactions in the cancer proteome† Cite this: Mol. BioSyst., 2016, 12,3067 David Xu,ab Shadia I. Jalal,c George W. Sledge Jr.d and Samy O. Meroueh*aef The Cancer Genome Atlas (TCGA) offers an unprecedented opportunity to identify small-molecule binding sites on proteins with overexpressed mRNA levels that correlate with poor survival. Here, we analyze RNA-seq and clinical data for 10 tumor types to identify genes that are both overexpressed and correlate with patient survival. Protein products of these genes were scanned for binding sites that possess shape and physicochemical properties that can accommodate small-molecule probes or therapeutic agents (druggable). These binding sites were classified as enzyme active sites (ENZ), protein–protein interaction sites (PPI), or other sites whose function is unknown (OTH). Interestingly, the overwhelming majority of binding sites were classified as OTH. We find that ENZ, PPI, and OTH binding sites often occurred on the same structure suggesting that many of these OTH cavities can be used for allosteric modulation of Creative Commons Attribution 3.0 Unported Licence. enzyme activity or protein–protein interactions with small molecules. We discovered several ENZ (PYCR1, QPRT,andHSPA6)andPPI(CASC5, ZBTB32,andCSAD) binding sites on proteins that have been seldom explored in cancer. We also found proteins that have been extensively studied in cancer that have not been previously explored with small molecules that harbor ENZ (PKMYT1, STEAP3,andNNMT) and PPI (HNF4A, MEF2B,andCBX2) binding sites. All binding sites were classified by the signaling pathways to Received 29th March 2016, which the protein that harbors them belongs using KEGG.
    [Show full text]
  • B-Cell Translocation Gene 2 Regulates Hepatic Glucose Homeostasis Via Induction of Orphan Nuclear Receptor Nur77 in Diabetic Mouse Model
    1870 Diabetes Volume 63, June 2014 Yong Deuk Kim,1 Sun-Gyun Kim,2 Seung-Lark Hwang,3 Hueng-Sik Choi,4 Jae-Hoon Bae,1 Dae-Kyu Song,1 and Seung-Soon Im1 B-Cell Translocation Gene 2 Regulates Hepatic Glucose Homeostasis via Induction of Orphan Nuclear Receptor Nur77 in Diabetic Mouse Model Diabetes 2014;63:1870–1880 | DOI: 10.2337/db13-1368 B-cell translocation gene 2 (BTG2) is a member of an might serve as a potential therapeutic target for com- emerging gene family that is involved in cellular func- bating metabolic dysfunction. tions. In this study, we demonstrate that BTG2 regu- lates glucose homeostasis via upregulation of Nur77 in diabetic mice. Hepatic BTG2 gene expression was The liver plays a crucial role in glucose homeostasis by elevated by fasting and forskolin. Overexpression of maintaining a balance between uptake and storage of Btg2 increased the expression of hepatic gluconeogenic glucose via glycogenesis and in the release of glucose by genes and blood glucose output and subsequently im- both glycogenolysis and gluconeogenesis (1,2). Gluconeo- paired glucose and insulin tolerance. Upregulation METABOLISM genesis is commonly triggered by key hormones including of the transcriptional activity of Nur77, gluconeogenic insulin, glucagon, and glucocorticoid, which contribute to genes, and glucose production by forskolin was ob- the expression of key metabolic enzymes, such as phospho- served by Btg2 transduction, but not in Btg2 knockdown. enolpyruvate carboxykinase (Pck1), fructose biphosphatase BTG2-stimulated glucose production and glucose-6- (Fbp) 1, and glucose-6-phosphatase (G6pc) (3). A variety phosphatase promoter activity were attenuated by of transcriptional factors and cofactors regulated by dominant-negative Nur77.
    [Show full text]
  • PEPCK): a Target of the P53-SIRT6-Foxo1 Axis
    LETTER LETTER Reply to Leithner et al.: Focus on phopshoenolpyruvate carboxykinase (PEPCK): A target of the p53-SIRT6-FoxO1 axis Cancer metabolism has drawn widespread attention has been paid to PCK2 because it in-depth discussion and further study. The attention since the unique metabolic proper- has proved to be less enzymatically active discrepancy between normal and tumor ties of cancer cells were characterized. We than PCK1, nevertheless, based on previous cells in PEPCK may provide a window found that p53, a potent tumor suppressor, studies of PCK2, we may make a bold spec- to understand cancer metabolism and the down-regulates the expression of phopshoe- ulation that PCK2 might be responsible for Warburg effect. nolpyruvate carboxykinase (PEPCK, PCK1) basal level gluconeogenesis and PCK1 for in coordination with histone deacetylase hormone and nutrient stimulation, with Zhiming Li a and Wei-Guo Zhua,b,1 sirtuin 6 (SIRT6) to inhibit tumor cell more flexible regulation. Given the facts that aKey Laboratory of Carcinogenesis and gluconeogenesis (1). Because p53 is malfunc- cancer cells have faster metabolism rates and Translational Research (Ministry of Education), tioning in numerous types of cancer, the PCK1 is absent or expressed in low levels in Department of Biochemistry and Molecular p53-SIRT6-forkhead box protein O1 (FoxO1) manyotherorgans,theseeminglymorefun- Biology, Peking University Health Science axis may shed light on the “Warburg effect,” damental PCK2 may be motivated in tumor Center, Beijing 100191, China Beijing 100191, a phenomenon in cancer metabolism that cells under glucose starvation. In addition, b has harassed scientists for decades. PCK1 is the general expression of PCK2 in most China; and Center for Life Sciences, Peking- mainly expressed in liver and kidney, with an organs other than liver, such as islets, and its Tsinghua University, Beijing 100871, China increasing expression and maturation after mitochondrial location, may endow it with birth.
    [Show full text]
  • Mitochondrial PCK2 Missense Variant in Shetland Sheepdogs with Paroxysmal Exercise-Induced Dyskinesia (PED)
    G C A T T A C G G C A T genes Article Mitochondrial PCK2 Missense Variant in Shetland Sheepdogs with Paroxysmal Exercise-Induced Dyskinesia (PED) 1, 2, 3 3 Jasmin Nessler y, Petra Hug y, Paul J. J. Mandigers , Peter A. J. Leegwater , Vidhya Jagannathan 2 , Anibh M. Das 4, Marco Rosati 5, Kaspar Matiasek 5, Adrian C. Sewell 6, Marion Kornberg 7, Marina Hoffmann 8 , Petra Wolf 9 , Andrea Fischer 10 , Andrea Tipold 1 and Tosso Leeb 2,* 1 Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany; [email protected] (J.N.); [email protected] (A.T.) 2 Institute of Genetics, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland; [email protected] (P.H.); [email protected] (V.J.) 3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands; [email protected] (P.J.J.M.); [email protected] (P.A.J.L.) 4 Department of Pediatrics, Hannover Medical School, 30625 Hannover, Germany; [email protected] 5 Section of Clinical and Comparative Neuropathology, Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universität, 80539 Munich, Germany; [email protected] (M.R.); [email protected] (K.M.) 6 Biocontrol, Labor für Veterinärmedizinische Diagnostik, 55218 Ingelheim, Germany; [email protected] 7 AniCura Tierklinik Trier GbR, 54294 Trier, Germany; [email protected] 8 Tierklinik Stommeln, 50259 Puhlheim, Germany; dr.marinahoff[email protected] 9 Nutritional Physiology and Animal Nutrition, University of Rostock, 18059 Rostock, Germany; [email protected] 10 Section of Neurology, Clinic of Small Animal Medicine, Ludwig-Maximilians-Universität, 80539 Munich, Germany; [email protected] * Correspondence: [email protected]; Tel.: +41-316-312-326 These authors contributed equally to this work.
    [Show full text]
  • The DNA Sequence and Comparative Analysis of Human Chromosome 20
    articles The DNA sequence and comparative analysis of human chromosome 20 P. Deloukas, L. H. Matthews, J. Ashurst, J. Burton, J. G. R. Gilbert, M. Jones, G. Stavrides, J. P. Almeida, A. K. Babbage, C. L. Bagguley, J. Bailey, K. F. Barlow, K. N. Bates, L. M. Beard, D. M. Beare, O. P. Beasley, C. P. Bird, S. E. Blakey, A. M. Bridgeman, A. J. Brown, D. Buck, W. Burrill, A. P. Butler, C. Carder, N. P. Carter, J. C. Chapman, M. Clamp, G. Clark, L. N. Clark, S. Y. Clark, C. M. Clee, S. Clegg, V. E. Cobley, R. E. Collier, R. Connor, N. R. Corby, A. Coulson, G. J. Coville, R. Deadman, P. Dhami, M. Dunn, A. G. Ellington, J. A. Frankland, A. Fraser, L. French, P. Garner, D. V. Grafham, C. Grif®ths, M. N. D. Grif®ths, R. Gwilliam, R. E. Hall, S. Hammond, J. L. Harley, P. D. Heath, S. Ho, J. L. Holden, P. J. Howden, E. Huckle, A. R. Hunt, S. E. Hunt, K. Jekosch, C. M. Johnson, D. Johnson, M. P. Kay, A. M. Kimberley, A. King, A. Knights, G. K. Laird, S. Lawlor, M. H. Lehvaslaiho, M. Leversha, C. Lloyd, D. M. Lloyd, J. D. Lovell, V. L. Marsh, S. L. Martin, L. J. McConnachie, K. McLay, A. A. McMurray, S. Milne, D. Mistry, M. J. F. Moore, J. C. Mullikin, T. Nickerson, K. Oliver, A. Parker, R. Patel, T. A. V. Pearce, A. I. Peck, B. J. C. T. Phillimore, S. R. Prathalingam, R. W. Plumb, H. Ramsay, C. M.
    [Show full text]
  • A MAFG-Lncrna Axis Links Systemic Nutrient Abundance to Hepatic Glucose Metabolism
    ARTICLE https://doi.org/10.1038/s41467-020-14323-y OPEN A MAFG-lncRNA axis links systemic nutrient abundance to hepatic glucose metabolism Marta Pradas-Juni et al.# Obesity and type 2 diabetes mellitus are global emergencies and long noncoding RNAs (lncRNAs) are regulatory transcripts with elusive functions in metabolism. Here we show that a high fraction of lncRNAs, but not protein-coding mRNAs, are repressed during diet-induced 1234567890():,; obesity (DIO) and refeeding, whilst nutrient deprivation induced lncRNAs in mouse liver. Similarly, lncRNAs are lost in diabetic humans. LncRNA promoter analyses, global cistrome and gain-of-function analyses confirm that increased MAFG signaling during DIO curbs lncRNA expression. Silencing Mafg in mouse hepatocytes and obese mice elicits a fasting-like gene expression profile, improves glucose metabolism, de-represses lncRNAs and impairs mammalian target of rapamycin (mTOR) activation. We find that obesity-repressed LincIRS2 is controlled by MAFG and observe that genetic and RNAi-mediated LincIRS2 loss causes elevated blood glucose, insulin resistance and aberrant glucose output in lean mice. Taken together, we identify a MAFG-lncRNA axis controlling hepatic glucose metabolism in health and metabolic disease. #A full list of authors and their affiliations appears at the end of the paper. NATURE COMMUNICATIONS | (2020) 11:644 | https://doi.org/10.1038/s41467-020-14323-y | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-020-14323-y ellular and organism-level energy homeostasis and nutri- by MAFG and CRISPR–Cas9-mediated knockout, or antisense- Cent partitioning are instrumental for survival. In higher mediated RNA interference of LincIRS2 causes hyperglycemia, organisms, multi-organ systems evolved to react to shifts insulin resistance, likely caused by alterations in glucogenic in energy supply by storing (anabolic) or metabolizing (catabolic) gene expression in lean mice.
    [Show full text]
  • Gluconeogenic Enzyme PCK1 Deficiency Promotes CHK2 O-Glcnacylation and Hepatocellular Carcinoma Growth Upon Glucose Deprivation
    The Journal of Clinical Investigation RESEARCH ARTICLE Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation Jin Xiang,1 Chang Chen,2 Rui Liu,1 Dongmei Gou,1 Lei Chang,3 Haijun Deng,1 Qingzhu Gao,1 Wanjun Zhang,3 Lin Tuo,4 Xuanming Pan,1 Li Liang,1 Jie Xia,1 Luyi Huang,1 Ke Yao,5 Bohong Wang,5 Zeping Hu,5 Ailong Huang,1 Kai Wang,1 and Ni Tang1 1Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, and 2Institute of Life Sciences, Chongqing Medical University, Chongqing, China. 3State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences, Beijing Institute of Lifeomics, Beijing, China. 4Sichuan Provincial People’s Hospital, Sichuan, China. 5School of Pharmaceutical Sciences, Tsinghua University, Beijing, China. Although cancer cells are frequently faced with a nutrient- and oxygen-poor microenvironment, elevated hexosamine- biosynthesis pathway (HBP) activity and protein O-GlcNAcylation (a nutrient sensor) contribute to rapid growth of tumor and are emerging hallmarks of cancer. Inhibiting O-GlcNAcylation could be a promising anticancer strategy. The gluconeogenic enzyme phosphoenolpyruvate carboxykinase 1 (PCK1) is downregulated in hepatocellular carcinoma (HCC). However, little is known about the potential role of PCK1 in enhanced HBP activity and HCC carcinogenesis under glucose-limited conditions. In this study, PCK1 knockout markedly enhanced the global O-GlcNAcylation levels under low-glucose conditions. Mechanistically, metabolic reprogramming in PCK1-loss hepatoma cells led to oxaloacetate accumulation and increased de novo uridine triphosphate synthesis contributing to uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) biosynthesis.
    [Show full text]
  • Novel Splice Variants of the Bovine PCK1 Gene
    Novel splice variants of the bovine PCK1 gene Z.B. Zhang1,2*, W. Zhang2*, R.L. Li2, J.B. Li2, J.F. Zhong2, Z.S. Zhao1 and J.M. Huang1 1College of Animal Science and Technology, Shihezi University, Shihezi, China 2Laboratory of Molecular Genetics and Breeding, Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China *These authors contributed equally to this study. Corresponding authors: Z. Zhao / J. Huang E-mail: [email protected] / [email protected] Genet. Mol. Res. 12 (3): 4028-4035 (2013) Received March 2, 2013 Accepted July 29, 2013 Published September 27, 2013 DOI http://dx.doi.org/10.4238/2013.September.27.4 ABSTRACT. Phosphoenolpyruvate carboxykinase 1 (PCK1), also named PEPCK-C, is a multiple-function gene that is involved in gluconeogenesis, glyceroneogenesis, reproduction, female fertility, and development of obesity and diabetes. How its many functions are regulated was largely unknown. Therefore, we investigated mRNA expression and possible splice variants of PCK1 by screening cDNA in nine tissues from Holstein bulls and cows. PCK1 mRNA was highly expressed in the liver, kidney, ovary and testis; expression levels were low in the heart, spleen, and lung tissues. Expression of this gene was not detected in skeletal muscle. This led to the discovery of five novel bovine splice variants, named PCK1-AS1-PCK1-AS5. In PCK1-AS1, 51 nucleotides in the interior of exon 2 were spliced out. In PCK1- AS2, exons 2 and 3 were altered by the alternative 3' and 5' splice sites, respectively. PCK1-AS3 was truncated from the 3' end of exon 2 to the 5' end of exon 4.
    [Show full text]
  • Gluconeogenesis in Cancer: Door Wide Open
    LETTER Gluconeogenesis in cancer: Door wide open Zhang et al. (1) make a valuable contribution activated in lung cancer cells under low aDivision of Pulmonology, Department of to the field of cancer metabolism, showing glucose and mediates conversion of lactate Internal Medicine, Medical University of Graz, that the tumor suppressor p53 down-regu- to phosphoenolpyruvate (3). However, we A-8036 Graz, Austria; and bLudwig lates the gluconeogenic key enzyme phospho- found that in lung cancer cells and in tumor Boltzmann Institute for Lung Vascular enolpyruvate carboxykinase (PEPCK, PCK1) samples PCK2 (PEPCK-M), the mitochon- Research, A-8036 Graz, Austria via histone deacetylase sirtuin 6. The authors drial isoform of the enzyme, and not PCK1, conclude that inhibition of gluconeogenesis the cytoplasmic isoform (PEPCK-C) studied may contribute to the tumor-suppressive by Zhang et al. (1), was expressed at high 1 Zhang P, et al. (2014) Tumor suppressor p53 cooperates with function of p53. Gluconeogenesis, a reverse levels. We further demonstrated that PCK2 SIRT6 to regulate gluconeogenesis by promoting FoxO1 nuclear exclusion. Proc Natl Acad Sci USA 111(29):10684–10689. glycolysis pathway, generates glucose from limits cancer cell death under low glucose 2 Schulze A, Harris AL (2012) How cancer metabolism is tuned for small carbohydrate precursors. The authors conditions and in 3D spheroids (3). Similar proliferation and vulnerable to disruption. Nature 491(7424):364–373. speculate that this is conceivably essential findings on PCK2 were obtained in breast, 3 Leithner K, et al. (2014) PCK2 activation mediates an adaptive response to glucose depletion in lung cancer. Oncogene, 10.1038/onc.2014.47.
    [Show full text]
  • Hyperinsulinemia Induces Insulin Resistance and Immune Suppression Via Ptpn6/Shp1 in Zebrafish
    R MARI´N-JUEZ and others Insulin-resistant zebrafish 222:2 229–241 Research Hyperinsulinemia induces insulin resistance and immune suppression via Ptpn6/Shp1 in zebrafish Correspondence 1 1 Rube´n Marı´n-Juez, Susanne Jong-Raadsen, Shuxin Yang and Herman P Spaink should be addressed to R Marı´n-Juez or H P Spaink ZF-Screens BV, J.H. Oortweg 19, 2333 CH Leiden, The Netherlands Emails 1Institute of Biology, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands [email protected] or h.p.spaink@biology. leidenuniv.nl Abstract Type 2 diabetes, obesity, and metabolic syndrome are pathologies where insulin resistance Key Words plays a central role, and that affect a large population worldwide. These pathologies are " hyperinsulinemia usually associated with a dysregulation of insulin secretion leading to a chronic exposure of " insulin resistance the tissues to high insulin levels (i.e. hyperinsulinemia), which diminishes the concentration " immune suppression of key downstream elements, causing insulin resistance. The complexity of the study of " ptpn6 insulin resistance arises from the heterogeneity of the metabolic states where it is observed. " zebrafish To contribute to the understanding of the mechanisms triggering insulin resistance, we have developed a zebrafish model to study insulin metabolism and its associated disorders. Zebrafish larvae appeared to be sensitive to human recombinant insulin, becoming Journal of Endocrinology insulin-resistant when exposed to a high dose of the hormone. Moreover RNA-seq-based transcriptomic profiling of these larvae revealed a strong downregulation of a number of immune-relevant genes as a consequence of the exposure to hyperinsulinemia. Interestingly, as an exception, the negative immune modulator protein tyrosine phosphatase nonreceptor type 6 (ptpn6) appeared to be upregulated in insulin-resistant larvae.
    [Show full text]
  • PPP1R3C Mediates Metformin-Inhibited Hepatic Gluconeogenesis
    Metabolism Clinical and Experimental 98 (2019) 62–75 Contents lists available at ScienceDirect Metabolism Clinical and Experimental journal homepage: www.metabolismjournal.com PPP1R3C mediates metformin-inhibited hepatic gluconeogenesis Xueying Ji a,1, Shushu Wang a,1,HongjuTanga,b,1,YuqingZhanga, Feiye Zhou a,LinlinZhanga, Qin Zhu a, Kecheng Zhu a,QianqianLiua,YunLiua, Xiao Wang a,⁎, Libin Zhou a,⁎ a Shanghai Institute of Endocrine and Metabolic Diseases, Department of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine andMetabolicDiseases,RuijinHospital,Shang- hai Jiaotong University School of Medicine, Shanghai 200025, PR China b The Department of Geriatrics, The People's of Wenshan Prefecture, Wenshan 663099, PR China article info abstract Article history: Background: Metformin has been widely used to alleviate hyperglycemia in patients with type 2 diabetes mainly Received 28 February 2019 via suppressing hepatic gluconeogenesis. However, the underlying mechanism remains incompletely clear. Here, Accepted 5 June 2019 we aimed to explore the role of PPP1R3C in metformin-mediated inhibition of hepatic gluconeogenesis. Methods: The differentially expressed genes in primary mouse hepatocytes incubated with 8-Br-cAMP and met- Keywords: formin were analyzed by microarrays. Hepatic glucose production and gluconeogenic gene expressions were de- PPP1R3C tected after adenovirus-mediated overexpression or silence of PPP1R3C in vitro and in vivo. The phosphorylation Metformin level and location of transducer of regulated CREB activity 2 (TORC2) were determined by Western blot and im- Gluconeogenesis fl AMPK muno uorescence. TORC2 Results: Metformin and adenovirus-mediated activation of AMPK suppressed 8-Br-cAMP–stimulated Ppp1r3c Liver mRNA expression in primary mouse hepatocytes. Overexpression of PPP1R3C in primary mouse hepatocytes or the livers of wild-type mice promoted hepatic glucose production and gluconeogenic gene expressions.
    [Show full text]