Title Ammonia Fungi
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Ammonia Fungi : A Chemoecological Grouping of Terrestrial Title Fungi Author(s) SAGARA, Naohiko Contributions from the Biological Laboratory, Kyoto Citation University (1975), 24(4): 205 Issue Date 1975-12-30 URL http://hdl.handle.net/2433/156000 Right Type Departmental Bulletin Paper Textversion publisher Kyoto University Contr. Biol. Lab. Kyoto Univ. 24 (4), 1975 AmmoniaFungi A Chemoecological Grouping of Terrestrial Fungi' Naohiko SAGARA CONTENTS Introduction.......................................,............................,.................................206 Historical .........................................................................................................207 I Occurrence of fungi after treatment with urea...................................................21O Methods................................................................................,.....................21O Experimental stands .............,...................,............................................21O Fieldexperiments....................................................................................213 Laboratory experiments...........................................................................214 Results and Discussion .........,...........,.........................,..,..............................217 Universality of the phenomena.....................,..........,.................................222 Specificity of the flora: "urea fungi" .............,........................................222 Groupings within the urea fungi ...........................................................,...223 Regional differences in the composition of urea fungi ,,..,............................224 Relationships with the type of vegetation .......................,.............,.............225 II Effects of some other agents on fungus flora .......,.,........,,.,,,.....,...........,............227 Methods...........................................................,..........................................227 Thc agents used ......................,......,.............,.....,.,,..,......,...........,.........227 Fieldexperiments............,,..........,.....,.......,.......,.....................................227 Laboratoryexperiments.......................,.,.,........,.,................,...................233 Resuks and Discussion .................................................................................234 Effects of each Agent group, with special reference to nitrogen and alkalinity......235 The essential factor for the succession of urea fungi ....................,..................242 Some secondary effects of the treatment .....,....................,........................242 III Natural habitats of the urea (proteophilous) fungi.............................................243 Methods.............................,............,...........................................................243 Results and Discussion .................................................................................243 IV Changes in soil properties and other organisms after the disturbances .....................246 Methods.................................,....................................................................2tl6 Examination of soil properties.........,..,.........,...........................................246 Observations on other organisms....................,..........................................246 Results and Discussion.....,..............................................................................246 Changes after urea treatment...................................,..............................246 Changes after treatment with some other agents.......................................250 Changes in the natural habitats............................................,...............252 V Taxonomic notes on each species and examination of their known habitats...............253 Zygomycetes......,........,,............,..................................................................253 Deuteromycetes.............................................................................................254 Ascomycetes ....,.,,......,...........................................................,........,............254 Basidiomycetes............,.,........,......,...........................,..................................260 * This work was partly supported by a Grant in Aid for Fundamental Scientific Research from the Ministry of Education, and formed a thesis submitted to Kyoto University for the degree of Doctor of Agriculture. 206 N. SAGARA Some findings .,...,.......................................................,.................,,..... ....264 Taxonomical.......................................................................,.....,..... ....264 Autoecological ................................................................................ ....265 Morphological or phylogenical .......................................,,.................. ....266 General discussion ...,..,................................................,...............,.........,.... ....266 A general view of the phenomena...,,............,................,.,,...........,... ....266 Relationships with known ecological groups.,............................,,...... ....267 New grouping of fungi ......,.......,..,..,.............................,................. ....269 Conclusions ...............................................,...................................,....,...... ....271 Summary ..........................................,..........,..................,...................,..... ....271 Acknowledgments....,...,.................,................................................,,.......,.,.. ....272 Literature cited.,.....,...,,.,..................,..,,..,........,.,.................,,.,,,...,..,...,...... ,.,,273 INTRODUCTION In a series of experiments to observe the response of higher fungi to chemical disturbance of forest soil (ground), urea application brought about a restricted and luxuriant occurrence (formation of reproductive structures on the soil surface)* of a few peculiar fungi, with all others disappearing, and some striking changes in soil properties (SAGARA & HAMADA, 1965). To elucidate the ecological meanings or causes of these phenomena, the following investigations were carried out. I. The urea effect was ascertained with the soils of various vegetations in many parts ofJapan and the fungi which showed the same or similar responses as above were listed. Further, modification of the urea effect by the region or by the type of vegetation was studied. II. Various kinds ofchemicals or agents were applied to soil to clarify the substi- tute for urea, the factors responsible for the urea effect, and the limits or extent of the phenomena in question. III. Natural habitats of the fungi obtained by the treatment with urea and its related materials were searched for in the field. IV. Responses of soil and other organisms to the chemical treatments and in the natural habitats detected by III were examined to determine the characteristics of the place of occurrence of the fungi in question. V. Taxonomic positions of these fungi were studied and their known habitats, which may suggest their ecological or physiological characters, were cited and discussed to delimit the findings. In these studies, the soils of uncultivated lands, especially of forests, were dealt with. Only the reproductive stages are discussed, though it has been observed on many occasions that a considerable vegetative growth do precede reproduction (Pl. 1, C; footnote on p. 269). Through these studies, I will propose an experimental-ecological * The words "occurrence (or to occur)" and "appearance (or to appear)" are used in this sense. The words "reproductive structures" indicate basidiocarps in Basidiomycetes, ascocarps in Ascomycetes, conidiophores and conidia in Deuteromycetes, and sporangiophores and sporangia in Zygomycetes. Further, the words "to yield (to produce, or to obtain) a species (or a fungus)" mean "to bring about the occurrence of a fungus species". Ammonia Fungi - A Chemoecologicat Crouping of Terrestrial Fungi 207 grouping of soil fungi (terrestrial fungi) as a step towards further studies. Succession (sequential appearance) of the fungi, fruit body production, effects of temperature (or season) and ofconcentration ofthe chemicals, etc. will not be discussed in detail in the present paper. An outline of the present contents was preliminarily published in SAGARA (1973). HISTORICAL It has not been well recognized that some fungi appear in response, direct or indirect, to chemical treatment ofsoil, and, despite considerable fertilization research, there has been relatively little literature on the occurrence of fungi. The information available is shown in Table 1. Excluded from Table I are the papers by GiLBERT (1875), RAuTAvAARA (1950), and FiEDLER & HuNGER (1963). GiLBERT observed, after repeated application of fertilizers to meadow-land for twenty years, a luxuriant occurrence of "Marasmius oreadum" in fairy rings exclusively "on the plot with superphosphate of lime, sulphate ofsodaand magnesia but without potass for fourteen years". RAuTAvAARA recognized that lime dust discharged from a nearby factory had some effects on the flora of larger fleshy fungi in a spruce forest. These are somewhat outside the scope of the present studies because their studies are concerned with the effects of long-term or continuous applications of chemicals (see p. 248 for the significance of