Comparative phylogeographic study of Hosta sieboldiana and Hosta albomarginata (Asparagaceae) in Japan Sangryong Lee & Masayuki Maki Botanical Gardens, Tohoku University, Kawauchi 12-2, Aoba-ku, Sendai 980-0862, Japan Keywords Abstract BARRIER, chloroplast DNA, Hosta albomarginata, Hosta sieboldiana, haplotype We analyzed variations in chloroplast DNA (cpDNA) in the widespread herba- diversity, Hosta, Japanese archipelago, NJ ceous species Hosta sieboldiana and Hosta albomarginata across large portions tree, phylogeography, SAMOVA. of their geographic ranges in the Japanese archipelago. Our objective was to compare the phylogeographic histories and phylogeographic structures of the Correspondence two congeneric species in the Japanese archipelago. The location of the study is Sangryong Lee, Botanical Gardens, Tohoku Japanese archipelago. We sequenced 1380 bp of noncoding cpDNA from 45 University, Aoba-ku, Sendai 980-0862, Japan. populations of H. sieboldiana (n = 362) and 55 populations of H. albomargi- Tel/Fax: +81-22-795-6789; = E-mail:
[email protected] nata (n 436) to assess genetic variations within and among populations across almost the entire distributions of the species in Japan. Extant patterns of Funding Information geographic structure were analyzed using statistical parsimony networks and This study was partly supported by a Grant- spatial analysis of molecular variance (SAMOVA). We also used Monmonier’s in-Aid from the Japan Ministry of Education, algorithm to detect genetic barriers between regions. Relationships between the Science, Sports and Culture to M.M. populations were examined using a neighbor-joining (NJ) method. Four haplo- types were found for H. sieboldiana, whereas eight haplotypes were identified Received: 7 March 2013; Revised: 12 September 2013; Accepted: 16 September for H.