Curriculum Vitae

Applicant (Last, First, Middle): Yanes, Oscar

CURRICULUM VITAE

NAME POSITION TITLE

Oscar Yanes Assistant Professor & Scientific Coordinator of

CIBERDEM and URV’s Metabolomics Platform.

EDUCATION & TRAINING: START END DEGREE (if SCIENTIFIC MONTH/ MONTH/ INSTITUTION AND LOCATION TRAINING MENTOR applicable) DISCIPLINE YEAR YEAR The Scripps Research Institute (La 1/2007 12/2010 Postdoctoral Dr. Gary Siuzdak Metabolomics Jolla, CA, USA) Ph.D. (FPI Autonomous University of 9/2001 10/2006 Prof. Francesc X. Aviles Biotechnology fellowship) Barcelona (Spain) Ph.D. (Exchange 9/2005 12/2005 PhD student, ETH Zurich (Zurich, Switzerland) Prof. Renato Zenobi Mass spectrometry FPI fellowship) Ph.D. (Exchange University of Southern Denmark Mass spectrometry- 8/2004 12/2004 PhD student, Prof. Peter Roepstorff (Odense, Denmark) based proteomics Marie Curie fellowship) Autonomous University of 9/1995 9/2000 Bachelor Barcelona (Spain) n.a. Biochemistry

PROFESSIONAL POSITIONS:

START END MONTH/ MONTH/ POSITION TITLE DEPARTMENT INSTITUTION AND LOCATION YEAR YEAR

4/2015 present Affiliated Member n.a. Institute for Research in Biomedicine (IRB) Barcelona (Barcelona, Spain) 1/2011 present Scientific Coordinator Metabolomics Platform Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM) (Madrid, Spain) 5/2011 present Assistant Professor Department of Universitat Rovira i Virgili (Tarragona, Electronic Engineering Spain)

Significant Professional Activities Include advisory panels, editorial boards, consulting, honors, awards (reverse chronological order)

Honors & Awards 2011: “Exceptional Junior Researcher” by CIBERDEM (Spanish Ministry of Science and Innovation). 2010: Outstanding Thesis Award, Universitat Autònoma de Barcelona (Spain). 2006: Spanish Proteomic Society award, Valencia (Spain). 2004: Marie Curie Training Site Fellowship.

Editorial Boards Associate Editor at Frontiers in Metabolomics (http://journal.frontiersin.org/journal/all/section/metabolomics)

Applicant (Last, First, Middle): Yanes, Oscar

Reviewing activities - Journals: Nature Biotechnology, Nature Nanotechnology, Nature Chemical Biology, Cell Metabolism, Angewandte Chemie International Edition, Nature Protocols, Chemistry & Biology, Analytical Chemistry, Scientific Reports, ACS Nano, Analytica Chimica Acta, Molecular Nutrition & Food Research, Journal of Proteome Research, PLoS ONE, and more. - Funding Agencies: Spanish National Evaluation and Foresight Agency (ANEP), Netherlands Organization for Scientific Research (NWO), Swiss National Science Foundation (SNSF).

Professional Association Membership 2001-present: Member, Spanish Society for Biochemistry and Molecular Biology (SEBBM). 2015-present: Member, Metabolomics Society. 2007-2012: Member, American Society for Mass Spectrometry (ASMS). 2009: Member, International Society for Stem Cell Research (ISSCR).

Organizing courses and workshops 2013: Organizer and instructor of the “Short-course in Metabolomics” (Centre for Omic Sciences, Reus, Spain). 2008-2010: Co-organizer and instructor of Short-courses in Mass Spectrometry, and Metabolomics (The Neurosciences Institute, La Jolla, California, USA).

Oral presentations at international conferences: 2016: FEAM (Federation of European Academies of Medicine) Spring Conference 2016, Bern (Switzerland) May 20th. 2013: (1) Plenary Lecture at the XIII Scientific Meeting of the Spanish Society of Chromatography and Related Techniques (SECyTA 2013), Tenerife (Spain), Oct 10th. (2) B-DEBATE International Center for Scientific Debate, Barcelona (Spain), Jan 16-17. 2012: 6th Santorini Conference Biologie Prospective, Systems Biology and Personalized Health Science and Translation. Santorini (Greece), 30 Sep-Oct 2. 2011: Agilent User Event at the Conference on Mass Spectrometry and Allied Topics (ASMS 2011), Denver (Colorado, USA), Jun 5-9. 2010: Mass Spectrometry: Applications to the Clinical Laboratory 2010 (MSACL '10). San Diego (CA, USA), Feb 6-10. 2008: Mass Spectrometry: Applications to the Clinical Laboratory 2008 (MSACL '08). San Diego (CA, USA), Nov 1-5.

Courses, Workshops & International advanced schools 2016: (1) Speaker at the EMBO Practical Course on Metabolomics Bioinformatics for Life Scientists, EMBL-EBI (UK) on Feb 14-19. (2) Speaker at the “Basic and Translational Research in Digestology” course of the Catalan Society of Digestology, Jan 28. 2015: (1) Speaker at the “Proteomics and Metabolomics course” of the Vall d'Hebron Research Institute (VHIR), March 9. (2) Speaker at the EMBO Practical Course on Metabolomics Bioinformatics for Life Scientists, EMBL-EBI (UK) on Feb 16-20. 2014: (1) Plenary talk at the Workshop “Metabolomics Approaches-Advanced Analytical Tools” (Italian Mass Spectrometry Society), Nov 10. (2) Speaker at the EMBO Practical Course on Metabolomics Bioinformatics for Life Scientists, EMBL-EBI (UK) on Mar 17-21. 2013: Speaker at the EMBO Practical Course on Metabolomics Bioinformatics for Life Scientists, EMBL-EBI (UK), 25 Feb - 1 Mar. 2012: Workshop on Recent Advances and Future Directions in Applied Metabolomics. University of Manchester (UK), Feb 1st. 2011: Workshop on Metabolomics at the Barcelona Biomedical Research Park (PRBB). Barcelona, Spain, Dec 13th 2010-2012: Instructor of the “Metabolomics short-course“ at the 58th 59th and 60th American Society for Mass Spectrometry (ASMS) Conference.

Invited presentations to Seminars & Meetings 2015: (1) Seminar “Metabolomics: cross-disciplinarity to understand biological systems and disease”, IDIBELL, Barcelona (Spain) Nov 10th. (2) Seminar “Patofisiologia molecular de la diabetis i l’obesitat”, Barcelona (Spain) May 15th. (3) Seminar at the San Raffaele Scientific Institute, Milan (Italy) Jan 19th, 2015. (4) First speaker of the Metabolomics Society Webinar series, Jan 29th (http://metabolomicssociety.org/resources/videos/emn-webinars-2015-public). 2013: (1) Seminar “Metabolism of Cancer” (Societat Catalana de Biologia), Barcelona (Spain) Dec 10th. (2) Seminar at the Institute of Photonic Sciences (ICFO), Castelldefels (Barcelona, Spain) Sep 19th. (3) Seminar “Omic Sciences, innovation and business” at the Centre for Omic Sciences (Reus, Spain), Jan 22th. 2012: (1) Seminar at the Institute of Predictive and Personalized Medicine of Cancer (IMPPC), Barcelona (Spain), March 19th. (2) Plenary lecture at the Agilent Nordic User Meeting. Gothenburg (Sweden), April 19th. (3) Seminars sessions: Professional career development. CEICS Nobel Campus. Chemistry for Life (1-4 July 2012, Tarragona, Spain. 2011: (1) Molecular Medicines Programme Seminar at the Institute for Research in Biomedicine (IRB Barcelona). Barcelona (Spain), May 18th. (2) 4th CIBERDEM Annual Meeting. Boi-Taull (Spain), Oct 23-26. 2009: The Stem Cell Interest Group meeting. The Salk Institute for Biological Studies, La Jolla (CA), May 20th.

Peer-Reviewed Publications 37. Temprano A, Sembongi H, Han GS, Sebastián D, Capellades J, Moreno C, Guardiola J, Wabitsch M, Richart C, Yanes O, Zorzano A, Carman GM, Siniossoglou S, Miranda M. “Redundant roles of the phosphatidate phosphatase family in triacylglycerol synthesis in human adipocytes“. Diabetologia. 2016 Jun 25. doi:10.1007/s00125-016-401. 36. Wright RH, Lioutas A, Le Dily F, Soronellas D, Pohl A, Bonet J, Nacht AS, Samino S, Font-Mateu J, Vicent GP, Wierer M, Trabado MA, Schelhorn C, Carolis C, Macias MJ, Yanes O, Oliva B, Beato M. “ADP-ribose-derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling“. Science. 2016 Jun 3;352(6290):1221-5. doi: 10.1126/science.aad9335.

Applicant (Last, First, Middle): Yanes, Oscar

35. Slebe F, Rojo F, Vinaixa M, García-Rocha M, Testoni G, Guiu M, Planet E, Samino S, Arenas EJ, Beltran A, Rovira A, Lluch A, Salvatella X, Yanes O, Albanell J, Guinovart JJ, Gomis RR. “FoxA and LIPG endothelial lipase control the uptake of extracellular lipids for breast cancer growth”. Nature Communications, 2016 Apr 5; 7:11199. doi: 10.1038/ncomms11199. 34. Amigó N, Mallol R, Heras M, Martínez-Hervás S, Blanco-Vaca F, Escolà-Gil JC, Plana N, Yanes O, Masana L, Correig X. “Lipoprotein hydrophobic core lipids are partially extruded to surface in smaller HDL: Herniated HDL, a common feature in diabetes“. Scientific Reports, 2016 Jan 18;6:19249. doi: 10.1038/srep19249. 33. Capellades J, Navarro M, Samino S, Garcia-Ramirez M, Hernandez C, Simo R, Vinaixa M, Yanes O. “geoRge: a computational tool to detect the presence of stable isotope labeling in LC/MS-based untargeted metabolomics.“ Analytical Chemistry, Dec 6 2015; doi: 10.1021/acs.analchem.5b03628. 32. Ceperuelo-Mallafre V, Ejarque M, Duran X, Montori-Grau M, Rodríguez MA, Yanes O, Núñez-Roa C, Roche K, Garrido-Sánchez L, Saez E, Tinahones FJ, Gómez-Foix A, Vendrell J, Fernández-Veledo S. “Adipose tissue glycogen accumulation is associated with obesity-linked inflammation in humans“. Molecular Metabolism, Jan 2016; doi: 10.1016/j.molmet.2015.10.001. 31. Vinaixa M, Schymanski EL, Neumann S, Navarro M, Salek RM, Yanes O. “Mass spectral databases for LC/MS and GC/MS-based metabolomics: state of the field and future prospects”. Trends in Analytical Chemistry, 78 April 2016; 23-35, doi:10.1016/j.trac.2015.09.005. 30. Samino S, Vinaixa M, Diaz M, Beltran A, Rodriguez MA, Mallol R, Heras M, Cabre A, Garcia L, Canela N, de Zegher F, Correig X, Ibañez L, Yanes O. “Metabolomics reveals impaired maturation of HDL particles in adolescents with hyperinsulinaemic androgen excess”. Scientific Reports, 2015; 5, 11496; doi: 10.1038/srep11496. 29. Valli A, Rodriguez M, Moutsianas L, Fischer R, Fedele V, Huang HL, Van Stiphout R, Jones D, Mccarthy M, Vinaxia M, Igarashi K, Sato M, Soga T, Buffa F, Mccullagh J, Yanes O, Harris A, Kessler B. “Hypoxia induces a lipogenic cancer cell phenotype via HIF1α- dependent and -independent pathways”. Oncotarget. 2015 Feb 10;6(4):1920-41. 28. Mallol R, Amigó N, Rodríguez MA, Heras M, Vinaixa M, Plana N, Rock E, Ribalta J, Yanes O, Masana L, Correig C. “Liposcale: a novel advanced lipoprotein test based on 2D diffusion-ordered H NMR spectroscopy”. Journal of Lipid Research, 2015 Jan 7. pii: jlr.D050120. [Epub ahead of print]. 27. Beltran A, Samino S, Yanes O. “Sample Preparation Methods for LC-MS-Based Global Aqueous Metabolite Profiling”. Methods Mol Biol. 2014; 1198:75-80. doi:10.1007/978-1-4939-1258-2_5. 26. Malpique R, Figueiredo H, Esteban Y, Rebuffat SA, Hanzu FA, Vinaixa M, Yanes O, Correig X, Barceló-Batllori S, Gasa R, Kalko SG, Gomis R. “Integrative analysis reveals novel pathways mediating the interaction between adipose tissue and pancreatic islets in obesity in rats”. Diabetologia, 2014 Jun; 57(6):1219-31. doi: 10.1007/s00125-014-3205-0. 25. Saez I, Duran J, Sinadinos C, Beltran A, Yanes O, Tevy MF, Martínez-Pons C, Milán M, Guinovart JJ. “Neurons have an active glycogen metabolism that contributes to tolerance to hypoxia”. J Cereb Blood Flow Metab, 2014, Feb 26. doi:10.1038/jcbfm.2014.33. 24. Yanes O. “Metabolomics: Playing piñata with single cells”. Nature Chemical Biology, 2013, 9(8): 471-3. 23. Vinaixa M, Samino S, Saez I, Duran J, Guinovart JJ, Yanes O. “A guideline to univariate statistical analysis for LC/MS-based untargeted metabolomics-derived data”. Metabolites, 2012, 2(4), 775-795; doi:10.3390/metabo2040775. 22. Beltran A, Suarez M, Rodríguez MA, Vinaixa M, Samino S, Arola L, Correig X, Yanes O. “Assessment of compatibility between extraction methods for NMR- and LC/MS-based metabolomics”. Analytical Chemistry, 2012 Jul 17; 84(14):5838-44. 21. Calavia R, Annanouch F, Correig X, Yanes O. “Nanostructure Initiator Mass Spectrometry for Tissue Imaging in Metabolomics: Future Prospects and Perspectives”. Journal of Proteomics, 2012 Aug 30;75(16): 5061-8. doi: 10.1016/j.jprot.2012.05.00. 20. Patti G.J., Yanes O., Siuzdak G. “Metabolomics: The Apogee of the Omic Trilogy”. Nature Reviews Molecular Cell Biology, 2012, 13; 263-269. doi: 10.1038/nrm3314. 19. Vinaixa M, Rodríguez MA, Samino S, Díaz M, Beltran A, Mallol R, Bladé C, Ibañez L, Correig X, Yanes O. “Metabolomics reveals reduction of metabolic oxidation in women with polycystic ovary syndrome after pioglitazone-flutamide-metformin polytherapy”. PLoS ONE, 2011; 6(12):e29052. 18. Panopoulos AD*, Yanes O*, Ruiz S, Kida Y, Diep D, Tautenhahn R, Herrerías A, Batchelder EM, Plongthongkum N, Lutz M, Berggren WT, Zhang K, Evans RM, Siuzdak G, Izpisua-Belmonte JC. “The metabolome of induced pluripotent stem cells reveals metabolic changes required for somatic cell reprogramming”. Cell Research, 2011 Nov 8. doi: 10.1038/cr.2011.177. (*equal contribution). 17. Marchetti V*, Yanes O*, Aguilar E, Friedlander D, Wong M, Naccache S, Nemerow G, Siuzdak G, Friedlander M. “Differential Macrophage Polarization promotes Tissue Remodeling and Repair in a Model of Ischemic Retinopathy”. Scientific Reports, 2011; 1 (76), doi:10.1038/srep00076. (*equal contribution) 16. Patti GJ*, Yanes O*, Shriver L, Courade JP, Tautenhahn R, Manchester M, Siuzdak G. “Metabolomics implicates altered sphingolipids in chronic pain of neuropathic origin”. Nature Chemical Biology, 2012 Jan 22. doi: 10.1038/nchembio.767. (*equal contribution). 15. Yanes O, Tautenhahn R, Patti GJ, Siuzdak G. “Expanding Coverage of the Metabolome for Global Metabolite Profiling”. Analytical Chemistry, 2011; 83(6): 2152-61. 14. Yanes O, Clark J, Wong D.M, Patti J.G., Sanchez-Ruiz A, Benton P, Trauger S.A, Desponts C, Ding S, Siuzdak G. “Metabolic oxidation regulates stem cell differentiation”. Nature Chemical Biology, 2010; 6(6): 411-7. 13. Patti G.J, Woo, H.K. Yanes O, Leah S, Diane T, Uritboonthai W, Apon J, Manchester M, Siuzdak G. “Detection of Carbohydrates and Steroids by Cation-enhanced Nanostructure-Initiator Mass Spectrometry (NIMS) for Biofluid Analysis and Tissue Imaging”. Analytical Chemistry, 2010; 82 (1): 121-8. 12. Yanes O, Woo H.K, Northen T.R, Oppenheimer S, Shriver L, Apon J, Estrada M.N, Potchoiba MJ, Steenwyk R, Manchester M, Siuzdak G. “Nanostructure Initiator Mass Spectrometry (NIMS): Tissue Imaging and Direct Biofluid Analysis”. Analytical Chemistry, 2009 (20).

Applicant (Last, First, Middle): Yanes, Oscar

11. Dorrell MI, Aguilar E, Jacobson R, Yanes O, Gariano R, Heckenlively J, Banin E, Ramirez G.A, Gasmi M, Bird A, Siuzdak G, Friedlander M. “Antioxidant or neurotrophic factor treatment preserves function in a mouse model of neovascularization-associated oxidative stress”. Journal of Clinical Investigation, 2009 (2). 10. Woo H.K, Northen T.R, Yanes O, Siuzdak G. “Nanostructure-Initiator Mass Spectrometry (NIMS): a protocol for preparing and applying NIMS surfaces for high sensitivity mass analysis”. Nature Protocols, 2008, 3(8):1341-9. 9. Northen TR*, Yanes O*, Northen MT, Marrinucci D, Uritboonthai W, Apon J, Golledge SL, Nordström A, Siuzdak G. “Clathrate nanostructures for mass spectrometry”. Nature, 2007, Oct 25; 449 (7165):1033-6. (*equal contribution). 8. Yanes O, Villanueva J, Querol E, Aviles F.X. “Detection of non-covalent protein interactions by ‘intensity fading’ MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors”. Nature Protocols, 2007, 2(1):119-30. 7. Yanes O, Wenzel R, Calvete J.J, Nazabal A, Aviles F.X., Zenobi R. “Proteomic profiling of a snake venom using cryodetection MALDI-TOF mass spectrometry”. J. Am. Soc. Mass Spectrom., 2007, Apr;18(4):600-6. 6. Yanes O, Aviles F.X., Roepstorff P, and Jørgensen T.J.D. “Exploring the ‘intensity fading’ phenomenon in the study of noncovalent interactions by MALDI-TOF mass spectrometry”. J. Am. Soc. Mass Spectrom., 2007, Feb;18(2):359-67. 5. Yanes O, Nazabal A, Wenzel R, Zenobi R, Aviles F.X. “Detection of Noncovalent Complexes in Biological Samples by Intensity Fading and High-Mass Detection MALDI-TOF mass spectrometry”. Journal of Proteome Research, 2006, 5(10), 2711-2719. 4. Ferrer-Navarro M, Gómez A, Yanes O, Planell R, Avilés F.X., Piñol J, Pérez Pons J.A., Querol E. “Proteome of the Bacterium Mycoplasma penetrans”. Journal of Proteome Research, 2006; 5(3) 688 – 694. 3. Yanes O, Villanueva J, Querol E, Avilés F.X. “Functional screening of serine protease inhibitors in the medical leech Hirudo medicinalis”. Molecullar & Cellular Proteomics 2005, 4, 1602-1613. 2. Yanes O, Villanueva J, Querol E, Avilés F.X. “Intensity-fading MALDI-TOF MS: novel screening for ligand binding and drug discovery”. Drug Discovery Today: TARGETS 2004, 3, S23. 1. Villanueva J*, Yanes O*, Querol E, Serrano L, Avilés F.X. “Identification of protein ligands in complex biological samples using Intensity-Fading MALDI-TOF Mass Spectrometry”. Analytical Chemistry 2003, 75, 3385-3395. (*equal contribution)

Active Grants 1. Title of the project: “Rethinking cellular metabolism through identification of unpredicted metabolites and biochemical transformations using a novel metabolomic approach”. Reference: BFU2014-57466. Principal investigator: Oscar Yanes. Funding Agency: Spanish Ministry of Economy and Competitiveness. Funding period: 01/01/2015 to 31/12/2017.

2. Title of the project: “ChroMe: Chromatin–metabolism interactions as targets for healthy living”. Reference: 675610. Principal investigator: Andreas Ladurner. Funding Agency: European Commission (H2020-MSCA-ITN-2015). Funding period: 15/02/2016 to 15/02/2019.