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Evaluation of Virulence and New Experimental Therapeutic Strategies for Emerging and Uncommon Medically Important Fungal Pathogens

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EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 Departament de Ciències Mèdiques Bàsiques EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Doctoral Thesis Directed by Drs. Emilio Mayayo Artal, Josep Guarro Artigas and Javier Capilla Luque 2013 UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 Departament de Ciències Mèdiques Bàsiques Facultat de Medicina i Ciències de la Salut Universitat Rovira i Virgili Drs. Emilio Mayayo Artal, Josep Guarro Artigas and Javier Capilla Luque, Professors of the Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili. Certify that: The present work entitled “Evaluation of virulence and new experimental therapeutic strategies for emerging and uncommom medically important fungal pathogens” presented by Fabiola Vanessa Fernández Silva to obtain the degree of Doctor by the Universitat Rovira i Virgili, has been carried out under their supervision at the Unitats d´Anatomia Patològica i Microbiologia, Departament de Ciències Mèdiques Bàsiques, and that it fulfills the requirements to obtain the European Doctorate mention. Reus, June 27, 2013 Emilio Mayayo Artal Josep Guarro Artigas Javier Capilla Luque UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 ACKNOWLEDGES It's time to start closing another stage, and to thank all those people who in one way or another have contributed to make it so. I would like to show my gratitude to Dr. Emilio Mayayo and Dr. Josep Guarro for his support, encouragement and guides during these years; and for giving me the opportunity to work in their lab. I would like to thank also to Dr. Javier Capilla for his assistance during this thesis. Thanks to Drs. María José Figueras. Josep Cano, Josepa Gené, Alberto Schitgel, Francisco Javier Pastor, and Roxana Beaz for the assistance and advice during these years. I would like to sincerely thank to Prof. Luis Zaror, and Prof. Heriberto Fernández for their recommendations and advices. I am very grateful to Prof. Cornelia Lass-Flörl and Dr. Michaela Lackner, (Innsbruck Medical University, Austria) for accepting me in their research group during my internship, for their concern, support at all times and the most important for returning the love for the research. I also want to express my gratitude to other people who helped and supported me during my stay at Innsbruck Medical University, Austria, Uli, Lisa, Betti and Emi. Este apartado se lo dedico con mucho cariño a todos aquellos amigos y compañeros que han estado presentes durante este camino que ha llegado a su fin. Vale y Eduardo por haber sido parte de los inicios de este trayecto y haberme ayudado a que fuera más ligero. Mi querida Faduis, quién lo hubiera pensado unos años atrás que gracias al doctorado nos volveríamos a encontrar, no tengo como agradecer tu apoyo, paciencia, tolerancia y amistad, no olvidaré todos los momentos compartidos, en especial aquellos de mambo, risas y sobre todo los de “enajenación mental temporal”. Mis queridas Haybrig y Caracolilla, mis incondicionales, una de las mejores cosas que me pasó durante este camino fue haberlas encontrado, gracias por haber estado allí con un abrazo o una sonrisa cada vez que fue necesario. Dania “mi amol” inolvidables UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING AND UNCOMMON MEDICALLY IMPORTANT FUNGAL PATHOGENS Fabiola Vanessa Fernández Silva Dipòsit Legal: T.1329-2013 ACKNOWLEDGES momentos vividos, gracias por cada mojito compartido!!!!. Mi querida Yas, cómo te podré olvidar? Todos los momentos vividos serán inolvidables e incontables jajajaja. Enrique, gracias por cada momento compartido por el apoyo, especialmente en los momentos de furia y por tu paciencia. Querido Marcelin supongo que podrás descansar un poco, ahora que habrá una loca menos que escuchar jajajajaja, gracias por tu paciencia (incalculable valor!!!!), por tus consejos y cafés compartidos, entre otros, jajajajajaja. Quién lo diría que en tan poco tiempo se podrían establecer lazos de amistad sincera, Sarita, Nuria, Iván, Nohorita, Pia muchas gracias por haber compartido su alegría y amistad durante la última etapa del “caminito”. También quiero agradecer a todas aquellas personas que compartieron conmigo durante estos 4 años en la URV, en particular a Carmen, Nuria y Caty , por su ayuda durante el desarrollo experimental de esta tesis y a su vez a Mar, Hugo, Margara, Aleja, Mabel, Julio, Esperanza , Juan, Braulio, Phil, Montsé e Inma. Amparito y Eduardo, mi cariño y agradecimiento son incalculables, gracias por permitirme ser una más de su familia. Estando tan lejos de mi hogar he comprendido el valor que tiene la amistad, que no importa ni la distancia ni el tiempo cuando es verdadera. Gracias mis queridos “amikos” (Carla, Marcy, Henry, Migue, Jona, Mauri, Mauro, Billy, Lore, Jessy, Pao, Paula, Taty, Celia, Carito, Mario and Mª Paz) simplemente por haber estado cada vez que los necesite, aunque haya sido por skype jajajaja. Este camino no podría haber llegado a su fin, sin haber contado con el incondicional apoyo de mi familia. Mis adorados hermanos gracias por haber estado cuando más los necesite. Hernán y Mafalda, mis queridos viejos, les agradezco infinitamente que nos hayan inculcado a que en la vida nada cae del cielo y que cada objetivo que uno se propone hay que conseguirlo con educación, trabajo y esfuerzo, pilares fundamentales que hoy me han permitido concluir esta etapa en mi vida profesional. ........GRACIAS!!!!!! UNIVERSITAT ROVIRA I VIRGILI EVALUATION OF VIRULENCE AND NEW EXPERIMENTAL THERAPEUTIC STRATEGIES FOR EMERGING
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