DOCSLIB.ORG

Sugarbeet Cyst Nematode

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Sugarbeet Cyst Nematode ICS 26 Jan 2017 Agrobiodiversity is a vital sub-set of biodiversity Crop species / varieties Wild harvested plants Crops Wild Species (CWR) Agrobiodiversity Landraces Livestock and fish species Biocontrol agents for crop/livestock pests Soil organisms in cultivated areas Pollinators Crop Wild Relatives (CWR) lentil (Lens culinaris Medik.) Crop wild relatives (CWR) are those taxa related to species (crops) with high socio- wheat (Triticum aestivum L.) economic importance The Western Mediterranean Region is one cabbage (Brassica oleracea L.) of the top biodiversity hotspots of Europe olive (Olea europaea L.) chickpea (Cicer arietinum L.) barley (Hordeum vulgare L.) almond (Prunus dulcis (Mill.) sugarbeet (Beta vulgaris L.) D.A. Webb Crop Wild Relatives: contain great genetic diversity than the crop species have successfully colonized adverse habitats, and they are repositories of beneficial traits lost by the crop species during domestication and breeding can contribute with useful genes: (i) pest and disease resistance (ii) abiotic tolerance (drought and salinity) CWR are potential gene donors to a crop It is essential to understand the diversity and distribution of the CWR and how closely a taxon is related to a crop for it to be considered a CWR SUGARBEET Study group: Sugarbeet species (Beta vulgaris spp. vulgaris ) Sugarbeet is the primary crop in the genus Beta L. Beta vulgaris spp. vulgaris is divided into four cultivar groups: All are derived from the wild form leaf beet garden beet fodder beet sugarbeet WILD FORM Beta L. - Subfamily Betoidea (Beta vulgaris spp. maritima ) (Amaranthaceae) Subfamily Betoideae: Taxonomy and distribution Beta-Patellifolia species from Portugal and Macaronesian Islands. (A–B). Beta vulgaris subsp . maritima (Portugal mainland); (C–D). Patellifolia patellaris (Sagres, Portugal Mainland); (E). P. procumbens (Cabo Verde); (F–G). B. macrocarpa (Tavira, Portugal mainland). How closely a taxon is related to a crop for be considered a CWR? ROMEIRAS M.M., et al. 2016 . PLoS ONE 11(3): e0152456 First step: getting data from National & European inventories Collaborative programme among most European countries: “Em Portugal a informação encontra-se dispersa (...) pelo que se justifica a atribuição de um elevado grau de prioridade à realização do inventário nacional” Getting data from biodiversity databases ° The centres of diversity are geographical areas where the genetic diversity of the crop and wild species is still thought to be concentrated ° Discovering richest regions in terms of CWR, allow us to efficiently plan conservation efforts to target them ° To gain insights into the effectiveness of ex-situ conservation efforts for the priority CWR taxa, data are frequently extracted from GBIF GBIF is one of the largest and most widely used biodiversity databases, with the objective to ‘make the world’s primary data on biodiversity freely and universally available via the Internet ’ Getting data from biodiversity databases Getting data from biodiversity databases Getting data from biodiversity databases Getting data from other databases Further information other databases Getting data from biodiversity databases Patellifolia : Patellifolia patellaris Patellifolia webbiana Patellifolia procumbens ROMEIRAS M.M., et al. 2016 . PLoS ONE 11(3): e0152456 Getting data from biodiversity databases Patellifolia can transmit traits providing resistance to serious diseases of sugarbeets: sugarbeet cyst nematode (Heterodera schachtii Schmidt) leaf spot disease (Cercospora beticola Sacc.) powdery mildew (Erysiphe polygoni DC.) CWR data extracted from GBIF: some examples Muito Obrigada! Maria [email protected] ICS, 26 Jan 2017.
Load more

Recommended publications
  • Genetic and Genomic Tools to Asssist Sugar Beet Improvement: the Value of the Crop Wild Relatives
    PERSPECTIVE published: 06 February 2018 doi: 10.3389/fpls.2018.00074 Genetic and Genomic Tools to Asssist Sugar Beet Improvement: The Value of the Crop Wild Relatives Filipa Monteiro 1,2*, Lothar Frese 3, Sílvia Castro 4, Maria C. Duarte 1, Octávio S. Paulo 1, João Loureiro 4 and Maria M. Romeiras 1,2* 1 Centre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências Universidade de Lisboa, Lisboa, Portugal, 2 Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal, 3 Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Quedlinburg, Germany, 4 Department of Life Sciences, Centre for Functional Ecology, Universidade de Coimbra, Coimbra, Portugal Sugar beet (Beta vulgaris L. ssp. vulgaris) is one of the most important European crops for both food and sugar production. Crop improvement has been developed to enhance productivity, sugar content or other breeder’s desirable traits. The introgression of traits from Crop Wild Relatives (CWR) has been done essentially for lessening biotic stresses Edited by: constraints, namely using Beta and Patellifolia species which exhibit disease resistance Piergiorgio Stevanato, characteristics. Several studies have addressed crop-to-wild gene flow, yet, for breeding Università degli Studi di Padova, Italy programs genetic variability associated with agronomically important traits remains Reviewed by: Martin Mascher, unexplored regarding abiotic factors. To accomplish such association from phenotype- Leibniz-Institut für Pflanzengenetik und to-genotype, screening for wild relatives occurring in habitats where selective pressures Kulturpflanzenforschung (IPK), are in play (i.e., populations in salt marshes for salinity tolerance; populations subjected Germany Chiara Broccanello, to pathogen attacks and likely evolved resistance to pathogens) are the most appropriate Università degli Studi di Padova, Italy streamline to identify causal genetic information.
    [Show full text]
  • Morphological and Molecular Characterization of Heterodera Schachtii and the Newly Recorded Cyst Nematode, H
    Plant Pathol. J. 34(4) : 297-307 (2018) https://doi.org/10.5423/PPJ.OA.12.2017.0262 The Plant Pathology Journal pISSN 1598-2254 eISSN 2093-9280 ©The Korean Society of Plant Pathology Research Article Open Access Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea Abraham Okki Mwamula1†, Hyoung-Rai Ko2†, Youngjoon Kim1, Young Ho Kim1, Jae-Kook Lee2, and Dong Woon Lee 1* 1Department of Ecological Science, Kyungpook National University, Sangju 37224, Korea 2Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea (Received on December 23, 2017; Revised on March 6, 2018; Accepted on March 13, 2018) The sugar beet cyst nematode, Heterodera schachtii population whereas those of H. schachtii were strongly is a well known pathogen on Chinese cabbage in the detected in H. schachtii monoxenic cultures. Thus, this highland fields of Korea. However, a race of cyst form- study confirms the coexistence of the two species in ing nematode with close morphological resemblance to some Chinese cabbage fields; and the presence of H. tri- H. trifolii was recently isolated from the same Chinese folii in Korea is reported here for the first time. cabbage fields. Morphological species differentiation between the two cyst nematodes is challenging, with Keywords : infective juvenile, morphometrics, vulval cone only minor differences between them. Thus, this study described the newly intercepted H. trifolii population, Handling Associate Editor : Lee, Yong Hoon and reviewed morphological and molecular charac- teristics conceivably essential in differentiating the two nematode species.
    [Show full text]
  • Expression Analysis of the Expanded Cercosporin Gene Cluster In
    EXPRESSION ANALYSIS OF THE EXPANDED CERCOSPORIN GENE CLUSTER IN CERCOSPORA BETICOLA A Thesis Submitted to the Graduate Faculty of the North Dakota State University of Agriculture and Applied Science By Karina Anne Stott In Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE Major Department: Plant Pathology May 2018 Fargo, North Dakota North Dakota State University Graduate School Title Expression Analysis of the Expanded Cercosporin Gene Cluster in Cercospora beticola By Karina Anne Stott The Supervisory Committee certifies that this disquisition complies with North Dakota State University’s regulations and meets the accepted standards for the degree of MASTER OF SCIENCE SUPERVISORY COMMITTEE: Dr. Gary Secor Chair Dr. Melvin Bolton Dr. Zhaohui Liu Dr. Stuart Haring Approved: 5-18-18 Dr. Jack Rasmussen Date Department Chair ABSTRACT Cercospora leaf spot is an economically devastating disease of sugar beet caused by the fungus Cercospora beticola. It has been demonstrated recently that the C. beticola CTB cluster is larger than previously recognized and includes novel genes involved in cercosporin biosynthesis and a partial duplication of the CTB cluster. Several genes in the C. nicotianae CTB cluster are known to be regulated by ‘feedback’ transcriptional inhibition. Expression analysis was conducted in wild type (WT) and CTB mutant backgrounds to determine if feedback inhibition occurs in C. beticola. My research showed that the transcription factor CTB8 which regulates the CTB cluster expression in C. nicotianae also regulates gene expression in the C. beticola CTB cluster. Expression analysis has shown that feedback inhibition occurs within some of the expanded CTB cluster genes.
    [Show full text]
  • Origin and Age of Australian Chenopodiaceae
    ARTICLE IN PRESS Organisms, Diversity & Evolution 5 (2005) 59–80 www.elsevier.de/ode Origin and age of Australian Chenopodiaceae Gudrun Kadereita,Ã, DietrichGotzek b, Surrey Jacobsc, Helmut Freitagd aInstitut fu¨r Spezielle Botanik und Botanischer Garten, Johannes Gutenberg-Universita¨t Mainz, D-55099 Mainz, Germany bDepartment of Genetics, University of Georgia, Athens, GA 30602, USA cRoyal Botanic Gardens, Sydney, Australia dArbeitsgruppe Systematik und Morphologie der Pflanzen, Universita¨t Kassel, D-34109 Kassel, Germany Received 20 May 2004; accepted 31 July 2004 Abstract We studied the age, origins, and possible routes of colonization of the Australian Chenopodiaceae. Using a previously published rbcL phylogeny of the Amaranthaceae–Chenopodiaceae alliance (Kadereit et al. 2003) and new ITS phylogenies of the Camphorosmeae and Salicornieae, we conclude that Australia has been reached in at least nine independent colonization events: four in the Chenopodioideae, two in the Salicornieae, and one each in the Camphorosmeae, Suaedeae, and Salsoleae. Where feasible, we used molecular clock estimates to date the ages of the respective lineages. The two oldest lineages both belong to the Chenopodioideae (Scleroblitum and Chenopodium sect. Orthosporum/Dysphania) and date to 42.2–26.0 and 16.1–9.9 Mya, respectively. Most lineages (Australian Camphorosmeae, the Halosarcia lineage in the Salicornieae, Sarcocornia, Chenopodium subg. Chenopodium/Rhagodia, and Atriplex) arrived in Australia during the late Miocene to Pliocene when aridification and increasing salinity changed the landscape of many parts of the continent. The Australian Camphorosmeae and Salicornieae diversified rapidly after their arrival. The molecular-clock results clearly reject the hypothesis of an autochthonous stock of Chenopodiaceae dating back to Gondwanan times.
    [Show full text]
  • Great Nutraceutical Potential of Bioactive Compounds from Beta Vulgaris Cicla and Rubra Paolino Ninfali1, Elena Antonini1
    Nutrafoods (2018) 17:75-81 ORIGINAL RESEARCH DOI 10.17470/NF-018-1002-2 Received: February 21, 2018 Accepted: March 21, 2018 Great nutraceutical potential of bioactive compounds from Beta vulgaris cicla and rubra Paolino Ninfali1, Elena Antonini1 Correspondence to: Paolino Ninfali - [email protected] Beta vulgaris subsp. cicla (BVc, leaf beet) and Beta vulgaris var. rubra (BVr, red beetroot) belong to the Keywords Amaranthaceae family and have been used for centuries as food and medicinal plants. The main bioac- ABSTRACT Anticancer tive phytochemicals of BVr are the betalains, a group of water-soluble pigments derived from betalamic acid, which are divided into two classes: the yellow/orange-coloured betaxanthins (BX) and the red/ Anti-inflammatory violet-coloured betacyanins (BC). The seeds, leaves and roots of BVc are rich in phenolic acids and Antioxidants apigenin-derived flavonoids, namely vitexin, vitexin-2-O-rhamnoside (VOR) and vitexina-2-O-xyloside Betalains (XVX). We isolated BVc and BVr phytochemicals in our laboratory and tested them individually and in Nutraceutical products combination for their anticancer and anti-inflammatory activity. In cancer cells, vitexin flavonoids were Vitexin flavonoids able to induce intrinsic apoptosis, while betalains induced extrinsic apoptosis. Combinations of two or three molecules exhibited synergistic antioxidant, anti-inflammatory and anticancer activity, particularly towards hepatic, intestinal and urinary bladder tumours. Introduction also called chard or spinach beet and grown for its leaves, is an important economic crop in many regions of Italy. Beta vulgaris subsp. vulgaris is a herbaceous biennial plant Beta vulgaris var. rubra (BVr, red beetroot) is widely culti- belonging to the order of the Caryophyllales, in the fam- vated in Northern and Central Italy for its dark red, yellow ily of the Amaranthaceae, and in the Betoideae subfamily or white roots.
    [Show full text]
  • Patellifolia (Chenopodiaceae)
    Development of SSR Markers for the Genus Patellifolia (Chenopodiaceae) Author(s): Marion Nachtigall, Lorenz Bülow, Jörg Schubert and Lothar Frese Source: Applications in Plant Sciences, 4(8) Published By: Botanical Society of America DOI: http://dx.doi.org/10.3732/apps.1600040 URL: http://www.bioone.org/doi/full/10.3732/apps.1600040 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. Applications in Plant Sciences 2016 4(8): 1600040 Applications in Plant Sciences PRIMER NOTE DEVELOPMENT OF SSR MARKErs FOR THE GENUS PATELLIFOLIA (CHENOPODIACEAE)1 MARION NACHTIGALL2, LORENZ BÜLOW2, JÖRG SCHUBERT3, AND LOTHAR FRESE2,4 2Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; and 3Institute for Biosafety in Plant Biotechnology, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Erwin-Baur-Str.
    [Show full text]
  • JOURNAL of NEMATOLOGY Morphological And
    JOURNAL OF NEMATOLOGY Article | DOI: 10.21307/jofnem-2020-098 e2020-98 | Vol. 52 Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands Phougeishangbam Rolish Singh1,2,*, Gerrit Karssen1, 2, Marjolein Couvreur1 and Wim Bert1 Abstract 1Nematology Research Unit, Heterodera dunensis n. sp. from the coastal dunes of Gran Canaria, Department of Biology, Ghent Canary Islands, is described. This new species belongs to the University, K.L. Ledeganckstraat Schachtii group of Heterodera with ambifenestrate fenestration, 35, 9000, Ghent, Belgium. presence of prominent bullae, and a strong underbridge of cysts. It is characterized by vermiform second-stage juveniles having a slightly 2National Plant Protection offset, dome-shaped labial region with three annuli, four lateral lines, Organization, Wageningen a relatively long stylet (27-31 µm), short tail (35-45 µm), and 46 to 51% Nematode Collection, P.O. Box of tail as hyaline portion. Males were not found in the type population. 9102, 6700, HC, Wageningen, Phylogenetic trees inferred from D2-D3 of 28S, partial ITS, and 18S The Netherlands. of ribosomal DNA and COI of mitochondrial DNA sequences indicate *E-mail: PhougeishangbamRolish. a position in the ‘Schachtii clade’. [email protected] This paper was edited by Keywords Zafar Ahmad Handoo. 18S, 28S, Canary Islands, COI, Cyst nematode, ITS, Gran Canaria, Heterodera dunensis, Plant-parasitic nematodes, Schachtii, Received for publication Systematics, Taxonomy. September
    [Show full text]
  • Plant Classification and Seeds
    Ashley Pearson – Plant Classification and Seeds ● Green and Gorgeous Oxfordshire ● Cut flowers ● Small amounts of veg still grown and sold locally Different Classification Systems Classification by Life Cycle : Ephemeral (6-8 wks) Annual Biennial Perennial Classification by Ecology: Mesophyte, Xerophyte, Hydrophyte, Halophyte, Cryophyte Raunkiaers Life Form System: based on the resting stage Raunkiaers Life Form System Classification contd. Classification by Plant Growth Patterns Monocots vs. Dicots (NB. only applies to flowering plants - angiosperms) Binomial nomenclature e.g., Beetroot = Beta vulgaris Plantae, Angiosperms, Eudicots, Caryophyllales, Amaranthaceae, Betoideae, Beta, Beta vulgaris After morphological characters, scientists used enzymes and proteins to classify plants and hence to determine how related they were via evolution (phylogeny) Present day we use genetic data (DNA, RNA, mDNA, even chloroplast DNA) to produce phylogenetic trees Plant part modifications ● Brassica genus ● ● Roots (swede, turnips) ● Stems (kohl rabi) ● Leaves (cabbages, Brussels Sprouts-buds) ● Flowers (cauliflower, broccoli) ● Seeds (mustards, oil seed rape) Plant hormones / PGR's / phytohormones ● Chemicals present in very low concentrations that promote and influence the growth, development, and differentiation of cells and tissues. ● NB. differences to animal hormones! (no glands, no nervous system, no circulatory system, no specific mode of action) ● Not all plant cells respond to hormones, but those that do are programmed to respond at specific points in their growth cycle ● Five main classes of PGR's ● Abscisic acid (ABA) ● Role in abscission, winter bud dormancy (dormin) ● ABA-mediated signalling also plays an important part in plant responses to environmental stress and plant pathogens ● ABA is also produced in the roots in response to decreased water potential.
    [Show full text]
  • A Synopsis of Chenopodiaceae Subfam. Betoideae and Notes on the Taxonomy of Beta
    Willdenowia 36 – 2006 9 GUDRUN KADEREIT, SANDRA HOHMANN & JOACHIM W. KADEREIT A synopsis of Chenopodiaceae subfam. Betoideae and notes on the taxonomy of Beta Abstract Kadereit, G., Hohmann, S. & Kadereit, J. W.: A synopsis of Chenopodiaceae subfam. Betoideae and notes on the taxonomy of Beta. – Willdenowia 36 (Special Issue): 9-19. – ISSN 0511-9618; © 2006 BGBM Berlin-Dahlem. doi:10.3372/wi.36.36101 (available via http://dx.doi.org/) A synopsis of the phylogeny and systematics of subfamily Betoideae of the Chenopodiaceae is pro- vided and a modified subfamilial classification proposed. Betoideae contain five or six genera, i.e. Beta, Patellifolia, Aphanisma, Oreobliton and Hablitzia. The inclusion of Acroglochin in Betoideae is not clearly resolved by molecular evidence. The five genera (excl. Acroglochin) fall into two clades. These are Beteae with Beta only, and Hablitzieae with the remaining four genera. Of these four genera, Patellifolia formerly has been regarded as a section of Beta (B. sect. Procumbentes). The closer rela- tionship of Patellifolia to Hablitzieae rather than to Beta is supported not only by molecular but also by flower morphological characters. Molecular evidence, in part newly generated, suggests that Beta can be divided into two well-supported groups. These are B. sect. Corollinae and B. sect. Beta. The often recognized unispecific B. sect. Nanae should be included in B. sect. Corollinae.InB. sect. Beta, proba- bly only two species, B. macrocarpa and B. vulgaris, should be recognized. Key words: angiosperms, beets, Patellifolia, phylogenetic systematics, ITS, morphology. Introduction The Betoideae are a small subfamily of the Amaranthaceae/Chenopodiaceae alliance, comprising between 11 and 16 species in five genera, dependent mainly on the classification of Beta L.
    [Show full text]
  • Evaluation of Rhizomania-Resistance Segregating Sequences and Overall Genetic Diversity Pattern Among Selected Accessions of Beta and Patellifolia
    Euphytica (2016) 207:685–706 DOI 10.1007/s10681-015-1570-5 Evaluation of rhizomania-resistance segregating sequences and overall genetic diversity pattern among selected accessions of Beta and Patellifolia. Potential implications of breeding for genetic bottlenecks in terms of rhizomania resistance Anna Litwiniec . Maria Gos´ka . Beata Choin´ska . Kamilla Kuzdowicz_ . Aleksander Łukanowski . Barbara Skibowska Received: 21 April 2015 / Accepted: 27 September 2015 / Published online: 7 October 2015 Ó The Author(s) 2015. This article is published with open access at Springerlink.com Abstract Sugar beet is hypothesized to have a representatives of Beta and Patellifolia and sugar beet narrowed genetic base due to its origin as White cultivars, with a special focus on the complex Beta Silesian Beet and from numerous breeding selections vulgaris. Another purpose was to determine the and practices. High sugar quality, yield of recoverable potential usefulness and conformity of selected sugar, cytoplasmic-male sterility system, monoger- molecular markers in different groups of materials in mity, pests and disease resistance and bolting resis- the context of rhizomania resistance. To reach these tance constitute some of the adaptations that goals, molecular RAPD, ISSR techniques, literature- significantly influenced the existing genetic back- selected rhizomania resistance-segregating sequences ground of the crop. In this study we aimed to evaluate as well as mitochondrial markers were used. The the extent of genetic diversity existing in wild beet comparison of genetic diversity in wild and cultivated Beta forms shows that the population differentiation values and distance values are relatively high in & A. Litwiniec ( ) Á B. Choin´ska cultivars. Moreover, the diversity component seemed Plant Breeding and Acclimatization Institute–National Research Institute, Bydgoszcz Research Center, to be compromised rather on the level of population Department of Genetics and Breeding of Root Crops, (Hs) than in total (Ht) in cultivars.
    [Show full text]
  • The IUCN Red List of Threatened Speciestm
    Species 2014 Annual ReportSpecies the Species of 2014 Survival Commission and the Global Species Programme Species ISSUE 56 2014 Annual Report of the Species Survival Commission and the Global Species Programme • 2014 Spotlight on High-level Interventions IUCN SSC • IUCN Red List at 50 • Specialist Group Reports Ethiopian Wolf (Canis simensis), Endangered. © Martin Harvey Muhammad Yazid Muhammad © Amazing Species: Bleeding Toad The Bleeding Toad, Leptophryne cruentata, is listed as Critically Endangered on The IUCN Red List of Threatened SpeciesTM. It is endemic to West Java, Indonesia, specifically around Mount Gede, Mount Pangaro and south of Sukabumi. The Bleeding Toad’s scientific name, cruentata, is from the Latin word meaning “bleeding” because of the frog’s overall reddish-purple appearance and blood-red and yellow marbling on its back. Geographical range The population declined drastically after the eruption of Mount Galunggung in 1987. It is Knowledge believed that other declining factors may be habitat alteration, loss, and fragmentation. Experts Although the lethal chytrid fungus, responsible for devastating declines (and possible Get Involved extinctions) in amphibian populations globally, has not been recorded in this area, the sudden decline in a creekside population is reminiscent of declines in similar amphibian species due to the presence of this pathogen. Only one individual Bleeding Toad was sighted from 1990 to 2003. Part of the range of Bleeding Toad is located in Gunung Gede Pangrango National Park. Future conservation actions should include population surveys and possible captive breeding plans. The production of the IUCN Red List of Threatened Species™ is made possible through the IUCN Red List Partnership.
    [Show full text]
  • Histopathology of Brassica Oleracea Var. Capitata Subvar
    Türk. entomol. derg., 2012, 36 (3): 301-309 ISSN 1010-6960 Orijinal araştırma (Original article) Histopathology of Brassica oleracea var. capitata subvar. alba infected with Heterodera cruciferae Franklin, 1945 (Tylenchida: Heteroderidae) Heterodera cruciferae Franklin, 1945 (Tylenchida: Heteroderidae) ile bulaşık Brassica oleracea var. capitata subvar. alba`nın histopatojisi Sevilhan MENNAN1* Zafar A. HANDOO2 Summary Anatomical changes induced by the cabbage cyst nematode (Heterodera cruciferae) have been insufficiently characterized. Here these changes were described in the root tissues of white head cabbage variety (Yalova F1) commonly grown in the Black Sea region of Turkey, where cabbage-growing areas are heavily infested. In glasshouse experiments conducted at 20 degrees C, susceptible white head cabbage seedlings were inoculated with 0 (untreated control) or 1000 juveniles/300 ml soil. Three, 6, 12, 24, 48, 72 h and 30 days after inoculations, two plants from each treatment were removed, embedded in paraffin by using microwave technique and then examined by photomicrography. Second-stage passed through the vascular system after root penetration and they started to feed as sedentary. In cross section of the roots, large cells in the cortex of infected plants were filled with moderately dense cytoplasm and the walls were heavily stained and ruptured. In longitudinal section, internal walls were perforated. Syncytia that had different degrees of vacuolization, and syncytial nuclei were hypertrophied and deeply indented. Contained conspicuous nucleoli were noticeable 24 h after inoculation. Syncytia originating from endodermal cells possessed ruptured walls around the feeding site of the developing juvenile. White females were observed on the roots 30 days after inoculation, a time at which plant height was reduced and root proliferation increased.
    [Show full text]