RT² Profiler PCR Array (Rotor-® Format) PANZ-087Z Rabbit Mitochondria RT2 Profiler PCR Array

Cat. no. 330231 PANZ-087ZR

For pathway expression analysis

Format For use with the following real-time cyclers

RT² Profiler PCR Array, Rotor-Gene Q, other Rotor-Gene cyclers Format R

Description

PANZ-087Z Rabbit Mitochondria RT² Profiler PCR Array

The Rabbit Mitochondria RT² Profiler PCR Array profiles the expression of 84 involved in the biogenesis and function of the cell's powerhouse organelle. The genes monitored by this array include regulators and mediators of mitochondrial molecular transport of not only the metabolites needed for the electron transport chain and oxidative phosphorylation, but also the ions required for maintaining the mitochondrial membrane polarization and potential important for ATP synthesis. Metabolism and energy production are not the only functions of mitochondria. Intrinsic pathway genes activated by intracellular damage signaling are also represented on this array. Despite the 's origins and its , the cellular genomic DNA encodes most of the proteins essential for mitochondrial replication and function. Therefore, this array's genes also target and translocate previously translated and folded proteins across and/or into the outer and/or inner mitochondrial membranes and even into the . A set of controls present on each array enables data analysis using the ∆∆CT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in the diverse cellular functions of mitochondrial biology with this array.

For further details, consult the RT² Profiler PCR Array Handbook.

Shipping and storage RT² Profiler PCR Arrays in the Rotor-Gene format are shipped at ambient temperature, on dry ice, or blue ice packs depending on destination and accompanying products.

For long term storage, keep plates at –20°C.

Note: Ensure that you have the correct RT² Profiler PCR Array format for your real-time cycler (see table above).

Note: Open the package and store the products appropriately immediately on receipt.

Sample & Assay Technologies Array layout

The 96 real-time assays in the Rotor-Gene format are located in wells 1–96 of the Rotor-Disc™ (plate A1–A12=Rotor-Disc 1–12, plate B1–B12=Rotor-Disc 13–24, etc.). To maintain data analysis compatibility, wells 97–100 do not contain real-time assays but will contain master mix to account for weight balance.

Gene table: RT² Profiler PCR Array

Position UniGene GenBank Symbol Description A01 N/A XM_002718439 AIFM2 Apoptosis-inducing factor, mitochondrion-associated, 2 A02 N/A XM_002719726 AIFM3 Apoptosis-inducing factor, mitochondrion-associated, 3 A03 N/A XM_002717648 AKT3 V-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) A04 Ocu.2468 NM_001082135 BCL2L1 BCL2-like 1 A05 Ocu.4500 NM_001171022 CAV2 Caveolin 2 A06 N/A XM_002715617 CPT2 Carnitine palmitoyltransferase 2 A07 Ocu.5412 XM_002714508 GCLC Glutamate-cysteine ligase, catalytic subunit A08 Ocu.6208 NM_001085444 GPX1 Glutathione peroxidase 1 A09 Ocu.1315 XM_002712368 HSPD1 Heat shock 60kDa protein 1 (chaperonin) A10 N/A XM_002709601 IMMT Inner membrane protein, mitochondrial LOC100337 A11 N/A XM_002712209 Metaxin 2-like 978 LOC100338 A12 N/A XM_002718896 Translocase of inner mitochondrial membrane 22 homolog 681 LOC100338 B01 Ocu.766 XM_002721722 Heat shock 90kDa protein 1, beta 694 B02 Ocu.5013 XM_002723644 TSPO Translocator protein (18kDa) LOC100338 B03 N/A XM_002712679 Dynamin 1-like 814 LOC100338 B04 N/A XM_002713423 Solute carrier family 25, member 26 902 LOC100338 B05 N/A XM_002718273 Translocase of inner mitochondrial membrane 9 homolog 928 LOC100338 B06 N/A XM_002712959 Solute carrier family 25, member 30 986 LOC100340 B07 N/A XM_002712024 Mitochondrial processing peptidase beta subunit 909 LOC100341 B08 Ocu.843 XM_002722836 Tetratricopeptide repeat domain 11-like 062 LOC100341 B09 Ocu.7208 XM_002719887 Ubiquitously-expressed transcript 210 LOC100341 B10 N/A XM_002720066 Translocase of inner mitochondrial membrane 23 (yeast) homolog 303 LOC100341 B11 N/A XM_002710299 GrpE-like 1, mitochondrial-like 336 LOC100342 B12 N/A XM_002714580 BCL2-antagonist/killer 1-like 046 LOC100342 C01 N/A XM_002722941 Mitochondrial thiamine pyrophosphate carrier-like 929 LOC100343 C02 N/A XM_002707971 Translocase of outer mitochondrial membrane 5 homolog 944 LOC100345 C03 Ocu.8385 XR_085360 Translocase of inner mitochondrial membrane 17 homolog B 112 LOC100345 C04 N/A XM_002721675 COX16 assembly homolog 540 LOC100346 C05 Ocu.7260 XM_002708437 Translocase of inner mitochondrial membrane 8 homolog b-like 423 LOC100346 C06 N/A XM_002716333 Mitofusin 1 474 LOC100346 Solute carrier family 25 (; adenine nucleotide translocator), C07 N/A XM_002717262 477 member 31 LOC100347 C08 N/A XM_002718845 Aryl hydrocarbon receptor interacting protein-like 1 082 LOC100347 C09 N/A XM_002718453 Solute carrier family 25, member 16 684 LOC100348 C10 N/A XM_002723787 BCL2/adenovirus E1B 19kD-interacting protein 3-like 536 Position UniGene GenBank Symbol Description LOC100348 C11 N/A XM_002715995 Translocase of inner mitochondrial membrane 8 homolog A-like 662 LOC100348 Solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), C12 N/A XM_002724430 722 member 10-like LOC100348 D01 N/A XM_002716338 DnaJ homolog, subfamily C, member 19 759 LOC100349 D02 N/A XM_002709160 Translocase of inner mitochondrial membrane 10 homolog 306 LOC100349 D03 N/A XM_002715147 Translocase of outer mitochondrial membrane 40-like 412 LOC100349 D04 N/A XM_002709955 Mpv17 protein 987 LOC100350 D05 N/A XM_002712180 Solute carrier family 25, member 12-like 328 LOC100351 D06 N/A XM_002722592 Solute carrier family 25, member 1 112 LOC100351 D07 N/A XM_002710131 Solute carrier family 25 member 2-like 232 LOC100351 D08 N/A XM_002713690 Solute carrier family 25, member 13-like 328 LOC100351 D09 Ocu.8423 XR_085313 Ras homolog gene family, member T1 349 LOC100352 D10 Ocu.5587 XM_002713398 Carnitine/acylcarnitine translocase 079 LOC100352 D11 N/A XM_002715852 Glutamate cysteine ligase, modifier subunit-like 174 LOC100352 D12 Ocu.7387 XM_002715522 SH3-containing protein SH3GLB1 421 LOC100352 E01 N/A XM_002718315 Translocase of outer mitochondrial membrane 20 homolog (yeast)-like 955 LOC100353 E02 Ocu.4623 XR_084882 Neurofilament, light polypeptide 68kDa 082 E03 Ocu.6054 XM_002723234 CPT1B Carnitine palmitoyltransferase 1B (muscle) LOC100353 E04 N/A XM_002722003 Translocase of inner mitochondrial membrane 44 480 LOC100353 E05 N/A XM_002718790 Ring finger protein 135-like 622 LOC100355 E06 Ocu.5663 XM_002708763 Fractured callus expressed transcript 1-like 100 LOC100355 E07 N/A XM_002717114 Mitochondrial COX18 311 LOC100355 E08 Ocu.626 XM_002718611 Cytochrome oxidase assembly factor-like 645 LOC100355 E09 N/A XM_002722547 BH3 interacting domain death agonist-like 991 LOC100356 E10 N/A XM_002711448 Translocase of inner mitochondrial membrane 50 homolog 545 LOC100356 COX10 homolog, cytochrome c oxidase assembly protein, heme A: E11 N/A XM_002718961 833 farnesyltransferase-like LOC100356 E12 N/A XM_002721098 Mitochondrial ornithine transporter 1 921 LOC100357 F01 Ocu.8285 XM_002709900 Leucine-rich PPR motif-containing protein 043 LOC100357 F02 Ocu.8400 XR_085162 Metaxin 1 146 LOC100358 F03 Ocu.3844 XM_002717598 Translocase of inner mitochondrial membrane 17 homolog A 025 LOC100358 F04 N/A XM_002716099 Stratifin-like 179 LOC100358 Solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, F05 N/A XM_002721410 473 34kDa), member 17 LOC100358 F06 N/A XM_002709031 Uncharacterized LOC100358499 499 LOC100358 F07 N/A XM_002721228 Translocase of outer mitochondrial membrane 34 561 LOC100359 F08 Ocu.4563 XM_002711214 Solute carrier family 25 member 3 028 LOC100359 F09 Ocu.3873 XM_002708700 2 188 F10 N/A XM_002712797 MIPEP Mitochondrial intermediate peptidase F11 N/A XM_002715413 MSTO1 Misato homolog 1 (Drosophila) Position UniGene GenBank Symbol Description F12 N/A XM_002716386 OPA1 Optic atrophy 1 (autosomal dominant) G01 N/A XM_002709377 PPARGC1A Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha G02 N/A XM_002720292 SLC25A14 Solute carrier family 25 (mitochondrial carrier, brain), member 14 G03 N/A XM_002722974 SLC25A25 Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 G04 N/A XM_002714467 SLC25A27 Solute carrier family 25, member 27 Solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), G05 Ocu.6278 NM_001082686 SLC25A4 member 4 G06 Ocu.2588 NM_001082627 SOD1 Superoxide dismutase 1, soluble G07 N/A XM_002719341 STARD3 StAR-related lipid transfer (START) domain containing 3 G08 Ocu.7569 NM_001171376 TAZ Tafazzin G09 Ocu.5741 NM_001171253 TOMM6 Translocase of outer mitochondrial membrane 6 homolog (yeast) G10 N/A XM_002716612 TOMM70A Translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) G11 Ocu.1779 NM_001082404 TP53 Tumor protein p53 G12 Ocu.2093 NM_001171077 UCP1 Uncoupling protein 1 (mitochondrial, proton carrier) H01 Ocu.2090 NM_001101682 ACTA2 Actin, alpha 2, smooth muscle, aorta H02 Ocu.734 NM_001101683 ACTB Actin, beta H03 Ocu.87 NM_001082253 GAPDH Glyceraldehyde-3-phosphate dehydrogenase H04 Ocu.1108 NM_001082277 LDHA Lactate dehydrogenase A LOC100346 H05 N/A XM_002723383 Non-POU domain containing, octamer-binding-like 936 H06 N/A SA_00511 NGDC Rabbit Genomic DNA Contamination H07 N/A SA_00104 RTC Reverse Transcription Control H08 N/A SA_00104 RTC Reverse Transcription Control H09 N/A SA_00104 RTC Reverse Transcription Control H10 N/A SA_00103 PPC Positive PCR Control H11 N/A SA_00103 PPC Positive PCR Control H12 N/A SA_00103 PPC Positive PCR Control Related products

For optimal performance, RT² Profiler PCR Arrays should be used together with the RT² First Strand Kit for cDNA synthesis and RT² SYBR® Green qPCR Mastermixes for PCR.

Product Contents Cat. no.

RT² First Strand Kit (12) Enzymes and reagents for cDNA synthesis 330401

RT² SYBR Green ROX™ FAST For 2 x 96 assays in 96-well plates; suitable for use 330620 Mastermix (2)* with the Rotor-Gene Q and other Rotor-Gene cyclers

* Larger kit sizes available; please inquire.

RT² Profiler PCR Array products are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen. com or can be requested from QIAGEN Technical Services or your local distributor.

Trademarks: QIAGEN®, Rotor-Gene®, Rotor-Disc™ (QIAGEN Group); ROX™ (Applera Corporation or its subsidiaries); SYBR® (Molecular Probes, Inc.). 1067688 03/2011 © 2011 QIAGEN, all rights reserved.

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Sample & Assay Technologies