Clemson University TigerPrints All Dissertations Dissertations 12-2013 Validation of Thermal Destruction of Pathogenic Bacteria in Rendered Animal Products Mary Hayes Clemson University,
[email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_dissertations Part of the Microbiology Commons Recommended Citation Hayes, Mary, "Validation of Thermal Destruction of Pathogenic Bacteria in Rendered Animal Products" (2013). All Dissertations. 1255. https://tigerprints.clemson.edu/all_dissertations/1255 This Dissertation is brought to you for free and open access by the Dissertations at TigerPrints. It has been accepted for inclusion in All Dissertations by an authorized administrator of TigerPrints. For more information, please contact
[email protected]. VALIDATION OF THERMAL DESTRUCTION OF PATHOGENIC BACTERIA IN RENDERED ANIMAL PRODUCTS A Dissertation Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy Microbiology by Mary Melissa Hayes December 2013 Accepted by: Annel K. Greene, Committee Chair Thomas A. Hughes Xuiping Jiang Paul L. Dawson ABSTRACT This study tested enumeration techniques for high fat food matrices and determined thermal death times in rendering animal products. Standard Class O phosphate/magnesium chloride dilution buffer series (Dilution Series A) and a modified (pre-warmed to 32ºC) lecithin phosphate dilution buffer series (Dilution Series B) were used to enumerate a Salmonella cocktail from both poultry and beef rendering materials. Results of this study indicate use of a modified lecithin buffer did not improve Salmonella enumeration accuracy from rendering materials. Instead, the results suggested use of xylose lysine deoxycholate agar (XLD) with either buffer system accurately enumerated Salmonella from rendering materials.