Master Position in Plant Molecular Biology

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Bachmair lab Master position in plant molecular biology A Master Thesis position is available in the Bachmair group (Max Perutz Labs), starting Dec 2020 or later. Topic A) Analysis of plant ubiquitin ligases via a synthetic biology approach in the budding yeast S. cerevisiae. We are analyzing protein turnover in plants that depends on amino-terminal degradation signals. Expression of the involved enzymes in yeast is part of mechanistic studies on these turnover pathways. B) Construction of expression vectors for novel reporter proteins (tandem fluorescent timer constructs). The vectors shall serve for analysis of half-life and subcellular localization of fused proteins. The constructs shall be tested in the model plant, A. thaliana. Focus of the Bachmair lab lies on the biochemical and cell biological analysis of protein turnover driven by amino-terminal degradation signals. The biological context is how plants respond to environmental stimuli such as heat, flooding or salt stress. Additional information can be found on the Bachmair lab web site (contact address below). Application Requirements: Finished study (B. Sc.) in Molecular Biology, Biochemistry or related, 45 ECTS accomplished in the Master module Duration: ca. 12 months Payment: acc. to FWF rates Contact: [email protected] Univ.-Prof. Dr. Andreas Bachmair Max Perutz Labs, Dept. of Biochemistry and Cell Biology, University of Vienna Rm. 5110, Dr.-Bohr-Gasse 9, A-1030 Wien https://www.maxperutzlabs.ac.at/research/research-groups/bachmair MAX PERUTZ LABS A joint venture of Part of Vienna BioCenter (VBC) • Dr.-Bohr-Gasse 9 • 1030 Vienna Tel: +43 1 4277 24001 • [email protected] www.maxperutzlabs.ac.at Recent publications (Bachmair lab) Millar, A. H., Heazlewood, J. L., Giglione, C., Holdsworth, M. J., Bachmair, A., Schulze, W. X. (2019) The scope, functions, and dynamics of posttranslational protein modifications. Annu Rev Plant Biol 70, 119-151. Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., Movahedi, M., Rooney, D., Gibbs, D. J., Smart, K., Bachmair, A., Gray, J. E., Dissmeyer, N., Castresana, C., Ray, R. V., Gevaert, K., Holdsworth, M. J. (2019) Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytol 221, 988-1000 Tomanov, K., Nehlin, L., Ziba, I., Bachmair, A. (2018) SUMO chain formation relies on the amino-terminal region of SUMO conjugating enzyme and has dedicated substrates in plants. Biochemical J 475, 61-68. Nukarinen, E., Tomanov, K., Ziba, I., Weckwerth, W., Bachmair, A. (2017) Protein sumoylation and phosphorylation intersect in Arabidopsis signaling. Plant J. 91, 505-517. About the Max Perutz Labs The Max Perutz Labs are a research institute established by the University of Vienna and the Medical University of Vienna to provide an environment for excellent, internationally recognized research and education in the field of Molecular Biology. Dedicated to a mechanistic understanding of fundamental biomedical processes, scientists at the Max Perutz Labs aim to link breakthroughs in basic research to advances in human health. The Max Perutz Labs are located at the Vienna BioCenter, one of Europe’s hotspots for Life Sciences, and host around 50 research groups, involving more than 450 scientists and staff from 40 nations. www.maxperutzlabs.ac.at PAGE 2 A joint venture of Part of Monday, October 5, 2020.

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Master's Position in Plant Molecular Biology
Bachmair Lab Master’s position in plant molecular biology About the Bachmair lab Focus of the Bachmair lab lies on the biochemical and cell biological analysis of protein turnover driven by amino-terminal degradation signals. The biological context is how plants respond to environmental stimuli such as heat, flooding, or salt stress. Additional information can be found on the group page of the Bachmair lab. About the research project The applicant shall analyze protein turnover that depends on amino-terminal degradation signals. Firstly, expression of plant ubiquitin ligases via a synthetic biology approach in the budding yeast S. cerevisiae serves to elucidate turnover mechanisms. Secondly, expression vectors for so-called tandem fluorescent timer reporters shall be made and tested in plants. These vectors serve to investigate tissue- specificity and subcellular localization of turnover pathways. Candidates Successful candidates should have finished studies in Molecular Biology, Biochemistry or related (B. Sc.), and have 45 ECTS accomplished in the Master module. Interest in plant signal transduction and cellular homeostasis is advantageous. Duration: Ca. 12 months, starting Sep 2021 or later Payment: According to FWF payment scheme Contact: Univ.-Prof. Dr. Andreas Bachmair [email protected] Max Perutz Labs, Dept. of Biochemistry and Cell Biology, Univ. Wien Rm. 5110, Dr.-Bohr-Gasse 9, A-1030 Wien About the Max Perutz Labs The Max Perutz Labs are a research institute established by the University of Vienna and the Medical University of Vienna to provide an environment for excellent, internationally recognized research and education in the field of Molecular Biology. Dedicated to a mechanistic understanding of fundamental biomedical processes, scientists at the Max Perutz Labs aim to link breakthroughs in basic research to advances in human health.
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