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Evaluating the Adaptive Evolutionary Convergence of Carnivorous Plant Taxa Through Functional Genomics
Evaluating the adaptive evolutionary convergence of carnivorous plant taxa through functional genomics Gregory L. Wheeler and Bryan C. Carstens Department of Evolution, Ecology, & Organismal Biology, The Ohio State University, Columbus, OH, United States of America ABSTRACT Carnivorous plants are striking examples of evolutionary convergence, displaying complex and often highly similar adaptations despite lack of shared ancestry. Using available carnivorous plant genomes along with non-carnivorous reference taxa, this study examines the convergence of functional overrepresentation of genes previously implicated in plant carnivory. Gene Ontology (GO) coding was used to quantitatively score functional representation in these taxa, in terms of proportion of carnivory- associated functions relative to all functional sequence. Statistical analysis revealed that, in carnivorous plants as a group, only two of the 24 functions tested showed a signal of substantial overrepresentation. However, when the four carnivorous taxa were analyzed individually, 11 functions were found to be significant in at least one taxon. Though carnivorous plants collectively may show overrepresentation in functions from the predicted set, the specific functions that are overrepresented vary substantially from taxon to taxon. While it is possible that some functions serve a similar practical purpose such that one taxon does not need to utilize both to achieve the same result, it appears that there are multiple approaches for the evolution of carnivorous function in plant genomes. Our approach could be applied to tests of functional convergence in other systems provided on the availability of genomes and annotation data for a group. Submitted 27 October 2017 Accepted 13 January 2018 Subjects Bioinformatics, Evolutionary Studies, Genomics, Plant Science Published 31 January 2018 Keywords Carnivorous plants, Gene Ontology, Functional genomics, Convergent evolution Corresponding author Gregory L. -
The Fairchild Tropical Garden NIXON SMILEY ______1
~GAZ.NE AMERICAN HORTI CULTURAL SOCIETY A vnion of the Ame'rican Horticultuml Society and the American Ho·rticultural Council 1600 BLADENSB URG ROAD, NORTHEAST . WASHINGTON 2, D. C. For Un ited H mticulture *** to accumulate, increase, and disseminate horticultuml infmmation B. Y. MORRISON, Editor Di?-ec to?'S T enns Expiring 1960 J AMES R. H ARLOW, Managing Editor D ONOVAN S. CORRELL T exas CARL "V. F ENN I NGER Editorial Committee Pennsylvania W. H . HODGE W'. H . HODGE, Chainnan Pen nS)1 Ivan i(~ ] OHN L. CREECH A. J. IRVI NG Yo?'k FREDElRI C P. L EE New "VILLIAM C. STEERE CONRAD B. LI NK New York CURTIS MAY FREDERICK G. MEYER T erms Ex1Jil'ing 1961 STUART M. ARMSTRONG 'WILBUR H. YOUNGMAN Maryland J OHN L. CREECH Maryland Officers 'WILLIAM H . FREDERICK, JR. DelawQ.j·e PR ES IDENT FRANCIS PATTESON-KNIGHT RICHARD P . 'WHITE V il'ginia Washington, D. C. DONALD WYMAN 111 assachv.setts FIRST VICE·PRESIDENT Tenns Expiring 1962 DONALD W YMAN Jamaica Plain, Massachusetts FREDERIC P. LEE Maryland HENRY T. SKINNER SECOND VICE- PRESIDENT Distl'ict of Columba STUART M. ARMSTRONG CEORGE H. SPALDING Silvel' Spring, Mal'yland California RICHARD P. WHITE SECRETARY-TREASURER District of Columbia OLIVE E. WEATHERELL AN NE " VERTSNER WOOD Washington, D. C. Pennsylvania The Amel'ican Ho'yticvltw'al Magazine is the official publication of the American Horticultural Society and is issued fo ur times a year during the q uarters commencing with January, April , July and October. It is devoted to the dissemination of knowledge in the science and art of growing ornamental plants, fruits, vegetables, and related subjects. -
A New Carnivorous Plant Lineage (Triantha) with a Unique Sticky-Inflorescence Trap
A new carnivorous plant lineage (Triantha) with a unique sticky-inflorescence trap Qianshi Lina,b,1, Cécile Anéc,d, Thomas J. Givnishc, and Sean W. Grahama,b aDepartment of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; bUBC Botanical Garden, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; cDepartment of Botany, University of Wisconsin–Madison, Madison, WI 53706; and dDepartment of Statistics, University of Wisconsin–Madison, Madison WI 53706 Edited by Elizabeth A. Kellogg, Donald Danforth Plant Science Center, St. Louis, MO, and approved June 5, 2021 (received for review October 30, 2020) Carnivorous plants consume animals for mineral nutrients that and in wetlands, including bogs, marly shorelines, and calcareous enhance growth and reproduction in nutrient-poor environments. spring-fed fens. In bogs, T. occidentalis is commonly found with Here, we report that Triantha occidentalis (Tofieldiaceae) represents recognized carnivorous plants such as Drosera rotundifolia a previously overlooked carnivorous lineage that captures insects on (Droseraceae) and Pinguicula vulgaris (Lentibulariaceae). During sticky inflorescences. Field experiments, isotopic data, and mixing the summer flowering season, T. occidentalis produces leafless models demonstrate significant N transfer from prey to Triantha, erect flowering stems up to 80 cm tall (12). These scapes have with an estimated 64% of leaf N obtained from prey capture in sticky glandular hairs, especially on their upper portions, a feature previous years, comparable to levels inferred for the cooccurring distinguishing Triantha from other genera of Tofieldiaceae round-leaved sundew, a recognized carnivore. N obtained via carnivory (Fig. 1). Small insects are often found trapped by these hairs; is exported from the inflorescence and developing fruits and may herbarium specimens are frequently covered in insects (Fig. -
Towards Resolving Lamiales Relationships
Schäferhoff et al. BMC Evolutionary Biology 2010, 10:352 http://www.biomedcentral.com/1471-2148/10/352 RESEARCH ARTICLE Open Access Towards resolving Lamiales relationships: insights from rapidly evolving chloroplast sequences Bastian Schäferhoff1*, Andreas Fleischmann2, Eberhard Fischer3, Dirk C Albach4, Thomas Borsch5, Günther Heubl2, Kai F Müller1 Abstract Background: In the large angiosperm order Lamiales, a diverse array of highly specialized life strategies such as carnivory, parasitism, epiphytism, and desiccation tolerance occur, and some lineages possess drastically accelerated DNA substitutional rates or miniaturized genomes. However, understanding the evolution of these phenomena in the order, and clarifying borders of and relationships among lamialean families, has been hindered by largely unresolved trees in the past. Results: Our analysis of the rapidly evolving trnK/matK, trnL-F and rps16 chloroplast regions enabled us to infer more precise phylogenetic hypotheses for the Lamiales. Relationships among the nine first-branching families in the Lamiales tree are now resolved with very strong support. Subsequent to Plocospermataceae, a clade consisting of Carlemanniaceae plus Oleaceae branches, followed by Tetrachondraceae and a newly inferred clade composed of Gesneriaceae plus Calceolariaceae, which is also supported by morphological characters. Plantaginaceae (incl. Gratioleae) and Scrophulariaceae are well separated in the backbone grade; Lamiaceae and Verbenaceae appear in distant clades, while the recently described Linderniaceae are confirmed to be monophyletic and in an isolated position. Conclusions: Confidence about deep nodes of the Lamiales tree is an important step towards understanding the evolutionary diversification of a major clade of flowering plants. The degree of resolution obtained here now provides a first opportunity to discuss the evolution of morphological and biochemical traits in Lamiales. -
Carnivorous Plant Newsletter Vol 48, No 2, June 2019
Byblis in cultivation in the tropics and in temperate climates Gregory Allan • Birmingham • United Kingdom • [email protected] Cindy Chiang • Singapore • [email protected] This article has been written based mostly on the authors’ experiences in growing Byblis in the UK and in Singapore. It is hoped that growers in regions with different climates will be able to extrapo- late from the information provided here, adjusting the methods described below to suit their own growing conditions. Hopefully much of the information provided here is universal in its application. Introduction If any genus of carnivorous plants deserves to be better known, both by horticulturalists and botanists, it is Byblis. The common name (ironically rarely used by enthusiasts) for the genus is “rainbow plants”, on account of the prismatic effect that can be produced when the sun shines on their copiously-produced sticky glands. All species follow a basic morphological plan: they have a central stem from which radiate numerous filiform leaves and scapes with showy flowers that typi- cally have purple petals (although white forms of most species are known) and vivid yellow anthers. Virtually all parts of the plants are covered in mucilage-tipped stalked glands (colloquially referred to as “tentacles”), which efficiently capture small insects, as well as microscopic sessile digestive glands. Another interesting characteristic of the genus is the presence of pulvini in most, if not all, species. Pulvini are swellings at the base of pedicels which, usually after successful pollination, uti- lize hydraulics to bend the pedicel, and consequently the developing fruit, downwards towards the ground. This phenomenon was discovered by Brian Barnes in the early 21st century. -
Floral Micromorphology and Nectar Composition of the Early Evolutionary Lineage Utricularia (Subgenus Polypompholyx, Lentibulariaceae)
Protoplasma https://doi.org/10.1007/s00709-019-01401-2 ORIGINAL ARTICLE Floral micromorphology and nectar composition of the early evolutionary lineage Utricularia (subgenus Polypompholyx, Lentibulariaceae) Bartosz J. Płachno1 & Małgorzata Stpiczyńska 2 & Piotr Świątek3 & Hans Lambers4 & Gregory R. Cawthray4 & Francis J. Nge5 & Saura R. Silva6 & Vitor F. O. Miranda6 Received: 1 April 2019 /Accepted: 4 June 2019 # The Author(s) 2019 Abstract Utricularia (Lentibulariaceae) is a genus comprising around 240 species of herbaceous, carnivorous plants. Utricularia is usually viewed as an insect-pollinated genus, with the exception of a few bird-pollinated species. The bladderworts Utricularia multifida and U. tenella are interesting species because they represent an early evolutionary Utricularia branch and have some unusual morphological characters in their traps and calyx. Thus, our aims were to (i) determine whether the nectar sugar concentrations andcompositioninU. multifida and U. tenella are similar to those of other Utricularia species from the subgenera Polypompholyx and Utricularia, (ii) compare the nectary structure of U. multifida and U. tenella with those of other Utricularia species, and (iii) determine whether U. multifida and U. tenella use some of their floral trichomes as an alternative food reward for pollinators. We used light microscopy, histochemistry, and scanning and transmission electron microscopy to address those aims. The concentration and composition of nectar sugars were analysed using high-performance liquid chroma- tography. In all of the examined species, the floral nectary consisted of a spur bearing glandular trichomes. The spur produced and stored the nectar. We detected hexose-dominated (fructose + glucose) nectar in U. multifida and U. tenella as well as in U. -
Branch Circus Flora and Fauna Survey PDF Document
FLORA AND VEGETATION SURVEY Branch Circus and Hammond Road, Success Prepared by: Prepared for: RPS MUNTOC PTY LTD AND 290 Churchill Avenue, SUBIACO WA 6008 SILVERSTONE ASSET PTY LTD PO Box 465, SUBIACO WA 6904 C/O Koltasz Smith T: 618 9382 4744 PO Box 127 F: 618 9382 1177 E: [email protected] BURSWOOD WA 6100 W: www.rpsgroup.com.au Report No: L07263 Version/Date: Rev 0, June 2008 RPS Environment Pty Ltd (ABN 45 108 680 977) Document Set ID: 5546761 Version: 1, Version Date: 31/01/2017 Flora and Vegetation Survey Branch Circus and Hammond Road, Success Document Status Review Format RPS Release Issue Version Purpose of Document Orig Review Date Review Approval Date Draft A Draft For Internal Review KelMcC VanYeo 30.04.08 Draft B Draft For Client Review VanYeo KarGod 14.05.08 SN 30.05.08 Rev 0 Final for Issue VanYeo 10.06.08 DC 12.06.08 B. Hollyock 13.06.08 Disclaimer This document is and shall remain the property of RPS. The document may only be used for the purposes for which it was commissioned and in accordance with the Terms of Engagement for the commission. Unauthorised copying or use of this document in any form whatsoever is prohibited. L07263, Rev 0, June 2008 DOCUMENT STATUS / DISCLAIMER Document Set ID: 5546761 Version: 1, Version Date: 31/01/2017 Flora and Vegetation Survey Branch Circus and Hammond Road, Success EXECUTIVE SUMMARY Flora A total of 229 taxa were recorded from the survey area, of which 155 or 68% were native. -
Byblis the Rainbow Plant
Byblis The Rainbow Plant For cultivation purposes Byblis can be split into two groups: peren- nial species and the annual species. The two perennial species B. gigantea and B. lamellata should be grown in a compost of one part by volume of moss peat or coir to one part sand. The plants can grow quite tall and become untidy and it is advisable to prune them occasionally. When pruning, al- ways cut back to an emerging bud on green wood. These two spe- cies seem very unwilling to produce new growth from woody mate- rial and can become quite tree-like after a few years. Plants can be propagated from cuttings (use the semi-hard green material taken from pruning) or by seed. Cuttings are best taken in spring. Byblis Gigantea —Canning Vale, Western Australia These species have an interesting means of releasing the pollen Copyright Allen Lowrie from their anthers. When a pollinating insect hovers over the flower the vibrations of its wings triggers the anthers to release pollen from small holes. In cultivation this can be replicatedby using a tuning fork. Strike the tuning fork and then touch the anthers. Pollen will be sprayed over the petals, and this can be trans- ferred to the female parts of the plant with a paintbrush or wooden cocktail stick. Seed will be ripe several months later and is best sown in early spring. Watering should be done by the tray system with the pots standing in a few centimetres during the summer months. In win- ter the compost should be kept slightly moist. -
The Linderniaceae and Gratiolaceae Are Further Lineages Distinct from the Scrophulariaceae (Lamiales)
Research Paper 1 The Linderniaceae and Gratiolaceae are further Lineages Distinct from the Scrophulariaceae (Lamiales) R. Rahmanzadeh1, K. Müller2, E. Fischer3, D. Bartels1, and T. Borsch2 1 Institut für Molekulare Physiologie und Biotechnologie der Pflanzen, Universität Bonn, Kirschallee 1, 53115 Bonn, Germany 2 Nees-Institut für Biodiversität der Pflanzen, Universität Bonn, Meckenheimer Allee 170, 53115 Bonn, Germany 3 Institut für Integrierte Naturwissenschaften ± Biologie, Universität Koblenz-Landau, Universitätsstraûe 1, 56070 Koblenz, Germany Received: July 14, 2004; Accepted: September 22, 2004 Abstract: The Lamiales are one of the largest orders of angio- Traditionally, Craterostigma, Lindernia and their relatives have sperms, with about 22000 species. The Scrophulariaceae, as been treated as members of the family Scrophulariaceae in the one of their most important families, has recently been shown order Lamiales (e.g., Takhtajan,1997). Although it is well estab- to be polyphyletic. As a consequence, this family was re-classi- lished that the Plocospermataceae and Oleaceae are their first fied and several groups of former scrophulariaceous genera branching families (Bremer et al., 2002; Hilu et al., 2003; Soltis now belong to different families, such as the Calceolariaceae, et al., 2000), little is known about the evolutionary diversifica- Plantaginaceae, or Phrymaceae. In the present study, relation- tion of most of the orders diversity. The Lamiales branching ships of the genera Craterostigma, Lindernia and its allies, hith- above the Plocospermataceae and Oleaceae are called ªcore erto classified within the Scrophulariaceae, were analyzed. Se- Lamialesº in the following text. The most recent classification quences of the chloroplast trnK intron and the matK gene by the Angiosperm Phylogeny Group (APG2, 2003) recognizes (~ 2.5 kb) were generated for representatives of all major line- 20 families. -
Roridula Gorgonias and a Non-Carnivorous Relative, Clethra Arborea
Mathematisch-Naturwissenschaftliche Fakultät Stefanie Hartmann | Michaela Preick | Silke Abelt | André Scheffel | Michael Hofreiter Annotated genome sequences of the carnivorous plant Roridula gorgonias and a non-carnivorous relative, Clethra arborea Suggested citation referring to the original publication: BMC Research Notes 13 (2020), Art. 426 DOI https://doi.org/10.1186/s13104-020-05254-4 ISSN 1756-0500 Postprint archived at the Institutional Repository of the Potsdam University in: Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe 1141 ISSN: 1866-8372 https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-503752 DOI: https://doi.org/10.25932/publishup-50375 Hartmann et al. BMC Res Notes (2020) 13:426 https://doi.org/10.1186/s13104-020-05254-4 BMC Research Notes RESEARCH NOTE Open Access Annotated genome sequences of the carnivorous plant Roridula gorgonias and a non-carnivorous relative, Clethra arborea Stefanie Hartmann1* , Michaela Preick1, Silke Abelt1, André Schefel2 and Michael Hofreiter1 Abstract Objective: Plant carnivory is distributed across the tree of life and has evolved at least six times independently, but sequenced and annotated nuclear genomes of carnivorous plants are currently lacking. We have sequenced and structurally annotated the nuclear genome of the carnivorous Roridula gorgonias and that of a non-carnivorous rela- tive, Madeira’s lily-of-the-valley-tree, Clethra arborea, both within the Ericales. This data adds an important resource to study the evolutionary genetics of plant carnivory across angiosperm lineages and also for functional and systematic aspects of plants within the Ericales. Results: Our assemblies have total lengths of 284 Mbp (R. gorgonias) and 511 Mbp (C. arborea) and show high BUSCO scores of 84.2% and 89.5%, respectively. -
The Roots of Carnivorous Plants
Plant and Soil (2005) 274:127–140 Ó Springer 2005 DOI 10.007/s11104-004-2754-2 The roots of carnivorous plants Wolfram Adlassnig1, Marianne Peroutka1, Hans Lambers2 & Irene K. Lichtscheidl1,3 1Institute of Ecology and Conservation Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria. 2School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, Crawley WA 6009, Australia. 3Corresponding author* Received 30 April 2004. Accepted in revised form 31 August 2004 Key words: carnivorous plants, insectivorous plants, morphology, nutrition, root Abstract Carnivorous plants may benefit from animal-derived nutrients to supplement minerals from the soil. Therefore, the role and importance of their roots is a matter of debate. Aquatic carnivorous species lack roots completely, and many hygrophytic and epiphytic carnivorous species only have a weakly devel- oped root system. In xerophytes, however, large, extended and/or deep-reaching roots and sub-soil shoots develop. Roots develop also in carnivorous plants in other habitats that are hostile, due to flood- ing, salinity or heavy metal occurance. Information about the structure and functioning of roots of car- nivorous plants is limited, but this knowledge is essential for a sound understanding of the plants’ physiology and ecology. Here we compile and summarise available information on: (1) The morphology of the roots. (2) The root functions that are taken over by stems and leaves in species without roots or with poorly developed root systems; anchoring and storage occur by specialized chlorophyll-less stems; water and nutrients are taken up by the trap leaves. (3) The contribution of the roots to the nutrient supply of the plants; this varies considerably amongst the few investigated species. -
Recircumscription of the Lecythidaceae
TAXON 47 - NOVEMBER 1998 817 Recircumscription of the Lecythidaceae Cynthia M. Morton'", Ghillean T. Prance', Scott A. Mori4 & Lucy G. Thorburn' Summary Morton. C. M.• Prance, G. T., Mori, S. A. & Thorburn. L. G.: Recircumscriplion of the Le cythidaceae. Taxon 47: 817-827. 1998. -ISSN 004Q-0262. The phylogenetic relationships of the genera of Lecythidaceae and representatives of Scyto petalaceae were assessed using cladistic analysis of both molecular (rbcL and trnL se quences) and morphological data. The results show that the pantropical family Lecythida ceae is paraphyletic. Support was found for the monophyly of three of the four subfamilies: Lecythidoideae, Planchonioideae, and Foetidioideae. The fourth subfamily, Napoleonaeol deae, was found to be paraphyletic, with members of the Scytopetalaceae being nested within it forming a strong clade with Asteranthos. Both families share a number of mor phological features, including several distinct characters such as cortical bundles in the stem. The combined analysis produced three trees of 471 steps and consistency index Cl = 0.71 and retention index Rl = 0.70. Asteranthos !'P.~, members of Scytopetalaceae should be treated as a subfamily of Lecythidaceae, while Napoleonaea and Crateranthus (the latter based solely on morphological features) should remain in the subfamily Napoleo naeoideae.The Lecythldaceaeare recircumscribed, and Asteranthosand members of Scyto peta/aceae are included in Scytopetaloideae. A formal·llWJ!J-pmic synopsis accommodating this new circumscription is presented. Introduction The Lecythidaceae Poit, are 8 pantropical family of trees and shrubs consisting of . 20 genera split into four subfamilies in contemporary classifications (Cronquist, 1981; Prance & Mori, 1979; Takhtajan, 1987; ~ri & Prance, 1990; Thome, 1992).