DOCSLIB.ORG

Information to Users

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Information to Users INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book. Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6” x 9” black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. u m T Bell & Howell Information and Leaming 300 North Zeeb Road, Ann Arbor. Ml 48106-1346 USA 800-521-0600 THE UNIVERSITY OF OKLAHOMA GRADUATE COLLEGE BIOENERGETIC PERSPECTIVES OF SYNTROPHIC SUBSTRATE DEGRADATION A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY by Bradley Eugene Jackson Norman, Oklahoma 1999 UMI Number; 9941854 UMI Microform 9941854 Copyright 1999, by UMI Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. UMI 300 North Zeeb Road Ann Arbor, Ml 48103 ©Copyright by Bradley Eugene Jackson 1999 All Rights Reserved BIOENERGETIC PERSPECTIVES OF SYNTROPHIC SUBSTRATE DEGRADATION A Dissertation Approved for the Department of Botany and Microbiology By Acknowledgements I am extremely grateful to the faculty and staff at the University of Oklahoma, especially within the Department of Botany and Microbiology, that provided me the opportunity to successfully complete my doctoral program. I would like to sincerely express my great appreciation to all members of my Ph. D. committee for their gracious time and advice. I am most joyously indebted to my major advisor. Professor Michael J. Mclnerney. Mike has been an unparalleled mentor, scholar, and scientist, and truly represents the best values from which a student can learn. I thank Mike for his unending support in the laboratory and for his years of friendship through undergraduate and postgraduate endeavors. I am deeply honored to have learned countless lessons in life and science from him. I offer a special thanks to Dr. Ralph Tanner. Ralph has given me a great perspective on maintaining focus and objectivity in science, but I have also benefited personally through many fruitful discussions regarding the nature of life, love, and happiness. My sincere thanks is given to Dr. Joseph Suflita. Joe has always offered direct, keen insight in all matters of science and has challenged me through constantly giving me a "hard time." A hearty thanks is given to Neil Wofford. He has been instrumental in my success through his countless tutorials on anaerobic technique. Probably more importantly. IV Neil provided a constant friendship, and we experienced many humorous, offbeat moments regarding matters that few others would think were “funny.” I thank my family and attribute much of my success to them. Their support has been overflowing with love, encouragement, money, and patience. Finally, my most passionate thank you is for my wife Jennifer. She has carried the burdens of many hardships when I was weak, and thanks to her miraculous partnership with me, I am a successful man. Table of Contents Acknowledgements .......................................................................................... tv List of Tables ...................................................................................................viii List of Figures ................................................................................................... x Preface ............................................................................................................ xii Chapter I Thermodynamic aspects of syntrophic benzoate degradation. Abstract .............................................................................................................1 Introduction .......................................................................................................2 Materials and Methods ......................................................................................5 Results ............................................................................................................. 8 Discussion ...................................................................................................... 21 References .....................................................................................................25 Chapter II The energetics of syntrophic butyrate degradation and its implications for anaerobic digestion processes. Abstract .......................................................................................................... 29 Introduction ..................................................................................................... 30 Materials and Methods .................................................................................... 34 Results ............................................................................................................ 36 Discussion ...................................................................................................... 44 References ..................................................................................................... 52 VI Chapter III Bacterial metabolism: Does a biological energy quantum exist? Abstract and Body ...........................................................................................59 References and Notes .....................................................................................72 Appendix Thiosulfate metabolism in Desulfotomaculum thermobenzoicum: Growth by disproportionation. Abstract .......................................................................................................... 78 Introduction ..................................................................................................... 79 Materials and Methods .................................................................................... 81 Results ............................................................................................................84 Discussion ...................................................................................................... 92 References ..................................................................................................... 95 Vll List of Tables Chapter I Table 1. The effect of acetate on the thermodynamics and threshold value of benzoate degradation by cocultures of S. aciditrophicus under methanogenic conditions ........................................................................................................... 13 Table 2. The effect of acetate on the thermodynamics and threshold value of benzoate degradation by cocultures of S. aciditrophicus under sulfate- reducing conditions .............................................................................................15 Table 3. The effect of acetate on the thermodynamics and threshold value of benzoate degradation by cocultures of S. aciditrophicus under nitrate- reducing conditions ............................................................................................ 17 Table 4. The effect of acetate on the thermodynamics and threshold value of benzoate degradation by cocultures of S. gentianae under methanogenic conditions ........................................................................................................... 19 Table 5. The effect of acetate on the thermodynamics and threshold value of benzoate degradation by cocultures of S. buswellii under sulfate-reducing conditions ...........................................................................................................20 V lll Chapter II Table 1. The effect of acetate on the thermodynamics and threshold value of butyrate degradation by cocultures of S. wolfei under methanogenic conditions ...........................................................................................................37 Table 2. The effect of acetate on the thermodynamics and threshold value of butyrate degradation by cocultures of S. wolfei under sulfate-reducing conditions ...........................................................................................................39 Table 3. The effect of acetate on the thermodynamics and threshold value of butyrate degradation by cocultures of S. wolfei under nitrate-reducing conditions ...........................................................................................................41 Chapter III Table 1. Reactions involved in syntrophic metabolism ......................................61 Table 2. The effect of acetate on the thermodynamics
Load more

Recommended publications
  • The 2014 Golden Gate National Parks Bioblitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event
    National Park Service U.S. Department of the Interior Natural Resource Stewardship and Science The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 ON THIS PAGE Photograph of BioBlitz participants conducting data entry into iNaturalist. Photograph courtesy of the National Park Service. ON THE COVER Photograph of BioBlitz participants collecting aquatic species data in the Presidio of San Francisco. Photograph courtesy of National Park Service. The 2014 Golden Gate National Parks BioBlitz - Data Management and the Event Species List Achieving a Quality Dataset from a Large Scale Event Natural Resource Report NPS/GOGA/NRR—2016/1147 Elizabeth Edson1, Michelle O’Herron1, Alison Forrestel2, Daniel George3 1Golden Gate Parks Conservancy Building 201 Fort Mason San Francisco, CA 94129 2National Park Service. Golden Gate National Recreation Area Fort Cronkhite, Bldg. 1061 Sausalito, CA 94965 3National Park Service. San Francisco Bay Area Network Inventory & Monitoring Program Manager Fort Cronkhite, Bldg. 1063 Sausalito, CA 94965 March 2016 U.S. Department of the Interior National Park Service Natural Resource Stewardship and Science Fort Collins, Colorado The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service.
    [Show full text]
  • Genome-Resolved Meta-Analysis of the Microbiome in Oil Reservoirs Worldwide
    microorganisms Article Genome-Resolved Meta-Analysis of the Microbiome in Oil Reservoirs Worldwide Kelly J. Hidalgo 1,2,* , Isabel N. Sierra-Garcia 3 , German Zafra 4 and Valéria M. de Oliveira 1 1 Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas–UNICAMP, Av. Alexandre Cazellato 999, 13148-218 Paulínia, Brazil; [email protected] 2 Graduate Program in Genetics and Molecular Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255, Cidade Universitária, 13083-862 Campinas, Brazil 3 Biology Department & CESAM, University of Aveiro, Aveiro, Portugal, Campus de Santiago, Avenida João Jacinto de Magalhães, 3810-193 Aveiro, Portugal; [email protected] 4 Grupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Microbiología, Universidad Industrial de Santander, Cra 27 calle 9, 680002 Bucaramanga, Colombia; [email protected] * Correspondence: [email protected]; Tel.: +55-19981721510 Abstract: Microorganisms inhabiting subsurface petroleum reservoirs are key players in biochemical transformations. The interactions of microbial communities in these environments are highly complex and still poorly understood. This work aimed to assess publicly available metagenomes from oil reservoirs and implement a robust pipeline of genome-resolved metagenomics to decipher metabolic and taxonomic profiles of petroleum reservoirs worldwide. Analysis of 301.2 Gb of metagenomic information derived from heavily flooded petroleum reservoirs in China and Alaska to non-flooded petroleum reservoirs in Brazil enabled us to reconstruct 148 metagenome-assembled genomes (MAGs) of high and medium quality. At the phylum level, 74% of MAGs belonged to bacteria and 26% to archaea. The profiles of these MAGs were related to the physicochemical parameters and recovery management applied.
    [Show full text]
  • Genomic Signatures of Predatory Bacteria
    The ISME Journal (2013) 7, 756–769 & 2013 International Society for Microbial Ecology All rights reserved 1751-7362/13 www.nature.com/ismej ORIGINAL ARTICLE By their genes ye shall know them: genomic signatures of predatory bacteria Zohar Pasternak1, Shmuel Pietrokovski2, Or Rotem1, Uri Gophna3, Mor N Lurie-Weinberger3 and Edouard Jurkevitch1 1Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Rehovot, Israel; 2Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel and 3Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel Predatory bacteria are taxonomically disparate, exhibit diverse predatory strategies and are widely distributed in varied environments. To date, their predatory phenotypes cannot be discerned in genome sequence data thereby limiting our understanding of bacterial predation, and of its impact in nature. Here, we define the ‘predatome,’ that is, sets of protein families that reflect the phenotypes of predatory bacteria. The proteomes of all sequenced 11 predatory bacteria, including two de novo sequenced genomes, and 19 non-predatory bacteria from across the phylogenetic and ecological landscapes were compared. Protein families discriminating between the two groups were identified and quantified, demonstrating that differences in the proteomes of predatory and non-predatory bacteria are large and significant. This analysis allows predictions to be made, as we show by confirming from genome data an over-looked bacterial predator. The predatome exhibits deficiencies in riboflavin and amino acids biosynthesis, suggesting that predators obtain them from their prey. In contrast, these genomes are highly enriched in adhesins, proteases and particular metabolic proteins, used for binding to, processing and consuming prey, respectively.
    [Show full text]
  • Research Article Review Jmb
    J. Microbiol. Biotechnol. (2017), 27(0), 1–7 https://doi.org/10.4014/jmb.1707.07027 Research Article Review jmb Methods 20,546 sequences and all the archaeal datasets were normalized to 21,154 sequences by the “sub.sample” Bioinformatics Analysis command. The filtered sequences were classified against The raw read1 and read2 datasets was demultiplexed by the SILVA 16S reference database (Release 119) using a trimming the barcode sequences with no more than 1 naïve Bayesian classifier built in Mothur with an 80% mismatch. Then the sequences with the same ID were confidence score [5]. Sequences passing through all the picked from the remaining read1 and read2 datasets by a filtration were also clustered into OTUs at 6% dissimilarity self-written python script. Bases with average quality score level. Then a “classify.otu” function was utilized to assign lower than 25 over a 25 bases sliding window were the phylogenetic information to each OTU. excluded and sequences which contained any ambiguous base or had a final length shorter than 200 bases were Reference abandoned using Sickle [1]. The paired reads were assembled into contigs and any contigs with an ambiguous 1. Joshi NA, FJ. 2011. Sickle: A sliding-window, adaptive, base, more than 8 homopolymeric bases and fewer than 10 quality-based trimming tool for FastQ files (Version 1.33) bp overlaps were culled. After that, the contigs were [Software]. further trimmed to get rid of the contigs that have more 2. Schloss PD. 2010. The Effects of Alignment Quality, than 1 forward primer mismatch and 2 reverse primer Distance Calculation Method, Sequence Filtering, and Region on the Analysis of 16S rRNA Gene-Based Studies.
    [Show full text]
  • 'Candidatus Desulfonatronobulbus Propionicus': a First Haloalkaliphilic
    Delft University of Technology ‘Candidatus Desulfonatronobulbus propionicus’ a first haloalkaliphilic member of the order Syntrophobacterales from soda lakes Sorokin, D. Y.; Chernyh, N. A. DOI 10.1007/s00792-016-0881-3 Publication date 2016 Document Version Accepted author manuscript Published in Extremophiles: life under extreme conditions Citation (APA) Sorokin, D. Y., & Chernyh, N. A. (2016). ‘Candidatus Desulfonatronobulbus propionicus’: a first haloalkaliphilic member of the order Syntrophobacterales from soda lakes. Extremophiles: life under extreme conditions, 20(6), 895-901. https://doi.org/10.1007/s00792-016-0881-3 Important note To cite this publication, please use the final published version (if applicable). Please check the document version above. Copyright Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons. Takedown policy Please contact us and provide details if you believe this document breaches copyrights. We will remove access to the work immediately and investigate your claim. This work is downloaded from Delft University of Technology. For technical reasons the number of authors shown on this cover page is limited to a maximum of 10. Extremophiles DOI 10.1007/s00792-016-0881-3 ORIGINAL PAPER ‘Candidatus Desulfonatronobulbus propionicus’: a first haloalkaliphilic member of the order Syntrophobacterales from soda lakes D. Y. Sorokin1,2 · N. A. Chernyh1 Received: 23 August 2016 / Accepted: 4 October 2016 © Springer Japan 2016 Abstract Propionate can be directly oxidized anaerobi- from its members at the genus level.
    [Show full text]
  • Microbial Analysis and Enrichment of Anaerobic Phenol and P-Cresol Degrading Consortia with Addition of AQDS
    © 2021 The Authors Water Science & Technology Vol 84 No 3, 683 doi: 10.2166/wst.2021.264 Microbial analysis and enrichment of anaerobic phenol and p-cresol degrading consortia with addition of AQDS Mingmei Chia, Xiaoli Sub, Xiaojiao Suna, Yan Xua, Xiaoxia Wanga and Yanling Qiua,* a School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China b Department of hematology, Xijing Hospital, Air Force Medical University, Xi’an 710032, China *Corresponding author. E-mail: [email protected] ABSTRACT Quinones and humus are ubiquitous in the biosphere and play an important role in the anaerobic biodegradation and biotransformation of organic acids, poisonous compounds as well as inorganic compounds. The impact of humic model compound, anthraquinone-2, 6-disulfonate (AQDS) on anaerobic phenol and p-cresol degradation were studied. Four methanogenic AQDS-free phenol and p-cresol enrich- ments and two phenol-AQDS enrichments were obtained using two sludges with potential biodegradability of phenol and cresol isomers as inoculum. 16S rRNA gene-cloning analysis combined with fluorescence in situ hybridization revealed that syntrophic aromatic compound degrading bacterium Syntrophorhabdus aromaticivorans was dominant in four AQDS-free enrichments, whereas phenol degrading Crypta- naerobacter phenolicus was dominant in two phenol-AQDS enrichments. Neither co-culture of S. aromaticivorans with Methanospirillum hungatei nor two phenol-AQDS enrichments could metabolize phenol using AQDS as the terminal electron acceptor. Further degradation experiments suggested that C. phenolicus related microbes in two phenol-AQDS enrichments were responsible for the conversion of phenol to benzoate, and benzoate was further degraded by benzoate degraders of Syntrophus aciditrophicus or Sporotomaculum syntrophicum to acetate.
    [Show full text]
  • 479683V2.Full.Pdf
    bioRxiv preprint doi: https://doi.org/10.1101/479683; this version posted July 25, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Syntrophus Conductive Pili Demonstrate that Common Hydrogen-Donating Syntrophs can 2 have a Direct Electron Transfer Option 3 4 David J.F. Walker1,2, Kelly P. Nevin1, Dawn E. Holmes1,3, Amelia-Elena Rotaru1,4, Joy E. 5 Ward1, Trevor L. Woodard1, Jiaxin Zhu5, Toshiyuki Ueki1, Stephen S. Nonnenmann2,5, Michael 6 J. McInerney6, and Derek R. Lovley1,2* 7 8 1Department of Microbiology, University of Massachusetts-Amherst, Amherst, MA, USA 9 2Institute for Applied Life Sciences, University of Massachusetts-Amherst 10 3Department of Physical and Biological Science, Western New England University, Springfield, 11 MA, USA 12 4Department of Biology, University of Southern Denmark, Odense, Denmark 13 5Department of Mechanical and Industrial Engineering, University of Massachusetts-Amherst 14 6Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA 15 *Corresponding author: [email protected] 16 bioRxiv preprint doi: https://doi.org/10.1101/479683; this version posted July 25, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 17 Abstract 18 Syntrophic interspecies electron exchange is essential for the stable functioning of diverse 19 anaerobic microbial communities. Hydrogen/formate interspecies electron transfer (HFIT), in 20 which H2 and/or formate function as diffusible electron carriers, has been considered to be the 21 primary mechanism for electron sharing because most common syntrophs were thought to lack 22 biochemical components, such as electrically conductive pili (e-pili), necessary for direct 23 interspecies electron transfer (DIET).
    [Show full text]
  • Phylogenetic Profile of Copper Homeostasis in Deltaproteobacteria
    Phylogenetic Profile of Copper Homeostasis in Deltaproteobacteria A Major Qualifying Report Submitted to the Faculty of Worcester Polytechnic Institute In Partial Fulfillment of the Requirements for the Degree of Bachelor of Science By: __________________________ Courtney McCann Date Approved: _______________________ Professor José M. Argüello Biochemistry WPI Project Advisor 1 Abstract Copper homeostasis is achieved in bacteria through a combination of copper chaperones and transporting and chelating proteins. Bioinformatic analyses were used to identify which of these proteins are present in Deltaproteobacteria. The genetic environment of the bacteria is affected by its lifestyle, as those that live in higher concentrations of copper have more of these proteins. Two major transport proteins, CopA and CusC, were found to cluster together frequently in the genomes and appear integral to copper homeostasis in Deltaproteobacteria. 2 Acknowledgements I would like to thank Professor José Argüello for giving me the opportunity to work in his lab and do some incredible research with some equally incredible scientists. I need to give all of my thanks to my supervisor, Dr. Teresita Padilla-Benavides, for having me as her student and teaching me not only lab techniques, but also how to be scientist. I would also like to thank Dr. Georgina Hernández-Montes and Dr. Brenda Valderrama from the Insituto de Biotecnología at Universidad Nacional Autónoma de México (IBT-UNAM), Campus Morelos for hosting me and giving me the opportunity to work in their lab. I would like to thank Sarju Patel, Evren Kocabas, and Jessica Collins, whom I’ve worked alongside in the lab. I owe so much to these people, and their support and guidance has and will be invaluable to me as I move forward in my education and career.
    [Show full text]
  • Geobacteraceae Are Important Members of Mercury-Methylating Microbial Communities of Sediments Impacted by Waste Water Releases
    The ISME Journal (2018) 12:802–812 https://doi.org/10.1038/s41396-017-0007-7 ARTICLE Geobacteraceae are important members of mercury-methylating microbial communities of sediments impacted by waste water releases 1 2 1 1 1 3 Andrea G. Bravo ● Jakob Zopfi ● Moritz Buck ● Jingying Xu ● Stefan Bertilsson ● Jeffra K. Schaefer ● 4 4,5 John Poté ● Claudia Cosio Received: 19 May 2017 / Revised: 29 September 2017 / Accepted: 18 October 2017 / Published online: 10 January 2018 © The Author(s) 2018. This article is published with open access Abstract Microbial mercury (Hg) methylation in sediments can result in bioaccumulation of the neurotoxin methylmercury (MMHg) in aquatic food webs. Recently, the discovery of the gene hgcA, required for Hg methylation, revealed that the diversity of Hg methylators is much broader than previously thought. However, little is known about the identity of Hg-methylating microbial organisms and the environmental factors controlling their activity and distribution in lakes. Here, we combined high-throughput sequencing of 16S rRNA and hgcA genes with the chemical characterization of sediments impacted by a 1234567890 waste water treatment plant that releases significant amounts of organic matter and iron. Our results highlight that the ferruginous geochemical conditions prevailing at 1–2 cm depth are conducive to MMHg formation and that the Hg- methylating guild is composed of iron and sulfur-transforming bacteria, syntrophs, and methanogens. Deltaproteobacteria, notably Geobacteraceae, dominated the hgcA carrying communities, while sulfate reducers constituted only a minor component, despite being considered the main Hg methylators in many anoxic aquatic environments. Because iron is widely applied in waste water treatment, the importance of Geobacteraceae for Hg methylation and the complexity of Hg- methylating communities reported here are likely to occur worldwide in sediments impacted by waste water treatment plant discharges and in iron-rich sediments in general.
    [Show full text]
  • Syntrophy in Anaerobic Global Carbon Cycles Michael J Mcinerney1, Jessica R Sieber1 and Robert P Gunsalus2
    Available online at www.sciencedirect.com Syntrophy in anaerobic global carbon cycles Michael J McInerney1, Jessica R Sieber1 and Robert P Gunsalus2 Syntrophy is an essential intermediary step in the anaerobic consumes the product with high affinity (Table 1). For conversion of organic matter to methane where metabolically example, the degradation of butyrate with hydrogen and distinct microorganisms are tightly linked by the need to acetate production is thermodynamically unfavorable maintain the exchanged metabolites at very low unless these metabolites are maintained at very low levels concentrations. Anaerobic syntrophy is thermodynamically by methanogens. This anaerobic metabolism, especially constrained, and is probably a prime reason why it is difficult to when methanogenesis is the driver of the terminal elec- culture microbes as these approaches disrupt consortia. tron accepting reactions, often involves consortia with Reconstruction of artificial syntrophic consortia has allowed tightly coupled syntrophic partnerships [1,2,3]. Syn- uncultured syntrophic metabolizers and methanogens to be trophic interactions also occur in sulfate-reducing optimally grown and studied biochemically. The pathways for environments as evidenced by sulfate-reducing consortia syntrophic acetate, propionate and longer chain fatty acid involved in anaerobic methane oxidation [4]. metabolism are mostly understood, but key steps involved in benzoate breakdown and cyclohexane carboxylate formation Anaerobic syntrophy differs from other types of microbial are unclear. Syntrophic metabolism requires reverse electron metabolism like aerobic fatty acid metabolism or deni- transfer, close physical contact, and metabolic synchronization trification in that a consortium of interacting microbial of the syntrophic partners. Genomic analyses reveal that species rather than a single microbial species is needed to multiple mechanisms exist for reverse electron transfer.
    [Show full text]
  • Provided for Non-Commercial Research and Educational Use Only. Not for Reproduction Or Distribution Or Commercial Use
    Provided for non-commercial research and educational use only. Not for reproduction or distribution or commercial use. This article was originally published by IWA Publishing. IWA Publishing recognizes the retention of the right by the author(s) to photocopy or make single electronic copies of the paper for their own personal use, including for their own classroom use, or the personal use of colleagues, provided the copies are not offered for sale and are not distributed in a systematic way outside of their employing institution. Please note that you are not permitted to post the IWA Publishing PDF version of your paper on your own website or your institution’s website or repository. Please direct any queries regarding use or permissions to [email protected] 1406 © IWA Publishing 2011 Water Science & Technology | 64.7 | 2011 Comparative 16S rRNA gene surveys of granular sludge from three upflow anaerobic bioreactors treating purified terephthalic acid (PTA) wastewater S. D. Perkins, N. B. Scalfone and L. T. Angenent ABSTRACT The microbial communities from three upflow anaerobic bioreactors treating purified terephthalic S. D. Perkins Department of Energy, acid (PTA) wastewater were characterized with 16S ribosomal RNA gene sequencing surveys. Environment and Chemical Engineering, Washington University in St. Louis, Universal bacterial and archaeal primers were used to compare the bioreactor communities to each St. Louis, MO 63130, USA other. A total of 1,733 bacterial sequences and 383 archaeal sequences were characterized. The high Syntrophus Pelotomaculum fi N. B. Scalfone number of spp. and spp. found within these reactors indicates ef cient L. T. Angenent (corresponding author) removal of benzoate and terephthalate.
    [Show full text]
  • Metagenomic Analyses of the Late Pleistocene Permafrost
    Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Biogeosciences Discuss., 12, 12091–12119, 2015 www.biogeosciences-discuss.net/12/12091/2015/ doi:10.5194/bgd-12-12091-2015 BGD © Author(s) 2015. CC Attribution 3.0 License. 12, 12091–12119, 2015 This discussion paper is/has been under review for the journal Biogeosciences (BG). Metagenomic Please refer to the corresponding final paper in BG if available. analyses of the late Pleistocene Metagenomic analyses of the late permafrost Pleistocene permafrost – additional E. Rivkina et al. tools for reconstruction of Title Page environmental conditions Abstract Introduction E. Rivkina1, L. Petrovskaya2, T. Vishnivetskaya3, K. Krivushin1, L. Shmakova1, Conclusions References 4,7 3 4,5,6 M. Tutukina , A. Meyers , and F. Kondrashov Tables Figures 1Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino, Russia J I 2Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia J I 3 University of Tennessee, Center for Environmental Biotechnology, Knoxville, USA Back Close 4Bioinformatics and Genomics Programme, Centre for Genomic Regulation (CRG), Barcelona, Spain Full Screen / Esc 5Universitat Pompeu Fabra (UPF), Barcelona, Spain 6Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain Printer-friendly Version 7Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia Interactive Discussion 12091 Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Received: 17 June 2015 – Accepted: 14 July 2015 – Published: 4 August 2015 Correspondence to: E. Rivkina ([email protected]) BGD Published by Copernicus Publications on behalf of the European Geosciences Union. 12, 12091–12119, 2015 Metagenomic analyses of the late Pleistocene permafrost E.
    [Show full text]