MBD1 (Methyl-Cpg-Binding Domain 1) Antibody

p. 1

MBD1 (Methyl-CpG-binding Domain 1)

Antibody ABGENT Purified Monoclonal Antibody (Mab) Vision Without Limits

Clone Name: 100B272 Catalog #: AM1036a Size: 0.1 mg Source: Mouse Lot #: SD21104D Concentration 0.25 mg/ml

Background: DNA methylation, or the addition of methyl groups to cytosine bases in Other Name: MBD1, PCM1 the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic Accession #: NP_056723 processes of methylation and demethylation. The demethylation enzyme was recently foundto be a mammalian protein, which exhibits Specificity: demethylase activity associated to a methyl-CpG-binding domain (MBD) Mouse: Positive (1). The enzyme is able to revert methylated cytosine bases to cytosines Human: Postive within the particular dinucleotide sequence mdCpdG by catalyzing the Other species: Not tested cleaving of the methyl group as methanol.MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to Applications: colocalize with foci of heavily methylated satellite DNA andbelieved to The suggested dilution will be: mediate the biological functions of the methylation signal. Surprisingly, ELISA 1:1,000 MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, Western blotting 1:100~500 MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, Immunohistochemistry Not tested but the expression of MBD1 and MBD2 is reduced or absent in Immunoprecipitation Not tested embryonic stemcells, which are known to be deficient in MeCP1 activity. Flow cytometry Not tested MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 Isotype: Mouse IgG1 is mutated at an exonic polynucleotide tract (4). Description: Keywords: This antibody was generated by immunizing mice with a synthetic peptide of human MBD1. DNA methylation, methyl-CpG-binding domain (MBD, mdCpdG, MeCP2, methylated DNA, MBD1, MBD2, MBD3, and MBD4 Storage: Maintain refrigerated at 2-8°C for up to 6 References: months. For long term storage store at –20°C. 1. Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397 (6720):579-583 1999. Format: 2. Hendrich B and Bird A. Mol Cell Biol, 18: 6538-6547(1998). Purified mouse monoclonal antibody supplied 3. Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, in PBS with 0.09% (W/V) sodium azide. This Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A. J Biol Chem 275 antibody is purified through a protein G (42): 32422-32429 (2000). column and eluted out with both high and low 4. Bader S, Walker M, Harrison D. Br J Cancer 83(12): 1646-1649 pH buffers and neutralized immediately after (2000). elution then followed by dialysis against PBS.

Precautions: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Related Products: 1. Dnmt1 Antibody: Cat#AM1032a 2. Dnmt2 Antibody: Cat#AP1021a 3. Dnmt3a Antibody: Cat#AM1034a 4. Dnmt3b Antibody: Cat#AM1035a 5. MBD1 Antibody: Cat#AM1036a 6. MBD2 Antibody: Cat#AM1037a 7. MBD3 Antibody: Cat#AM1038a 8. MBD4 Antibody: Cat#AM1039a 9. MIZF Antibody: Cat#AP1022a 10. TMS1 Antibody: Cat#AP1023a

Western blot analysis of MBD1 in HeLa cell lysate with anti-MBD1 mAb (Cat#AM1036) at a dilution of 2 ug/ml (lane 1) and 1 ug/ml (lane 2). A protein band of approximate MW of 70 kDa was detected. p. 2