Human SETD7 / SET9 - Purified

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OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] SA6049X Human SETD7 / SET9 - Purified Alternate names: H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N- methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain- containing protein 7, SET7, SET7/9 Quantity: 0.5 mg Concentration: 1.0 mg/ml (determined by Bradford assay) Background: SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino- terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET 7/9, unlike most other SET proteins, is exclusively a mono-methylase. Uniprot ID: Q8WTS6 NCBI: NP_085151.1 GeneID: 80854 Species: Human Source: E. coli Format: State: Liquid Purity: >95% by SDS-PAGE Buffer System: 50 mM Tris-HCl buffer (pH7.5) 0.2M NaCl, 5mM DTT, 20% glycerol Description: Recombinant human SET7/9, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. AA Sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK Molecular weight: 40.7 kDa (366 amino acids) Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Bing xiao. et al(2003) Nature 421(50)652-656. 2. Taewoo Kwon. et al(2003) EMBO 22(2) 292-303. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170928 1 / 2 SA6049X: Human SETD7 / SET9 - Purified Pictures: For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170928 2 / 2 Powered by TCPDF (www.tcpdf.org).

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The Set7 Lysine Methyltransferase Regulates Plasticity in Oxidative Phosphorylation Necessary for Trained Immunity Induced by Β-Glucan
University of Southern Denmark The Set7 Lysine Methyltransferase Regulates Plasticity in Oxidative Phosphorylation Necessary for Trained Immunity Induced by β-Glucan Keating, Samuel T.; Groh, Laszlo; van der Heijden, Charlotte D.C.C.; Rodriguez, Hanah; dos Santos, Jéssica C.; Fanucchi, Stephanie; Okabe, Jun; Kaipananickal, Harikrishnan; van Puffelen, Jelmer H.; Helder, Leonie; Noz, Marlies P.; Matzaraki, Vasiliki; Li, Yang; de Bree, L. Charlotte J.; Koeken, Valerie A.C.M.; Moorlag, Simone J.C.F.M.; Mourits, Vera P.; Domínguez-Andrés, Jorge; Oosting, Marije; Bulthuis, Elianne P.; Koopman, Werner J.H.; Mhlanga, Musa; El-Osta, Assam; Joosten, Leo A.B.; Netea, Mihai G.; Riksen, Niels P. Published in: Cell Reports DOI: 10.1016/j.celrep.2020.107548 Publication date: 2020 Document version: Final published version Document license: CC BY-NC-ND Citation for pulished version (APA): Keating, S. T., Groh, L., van der Heijden, C. D. C. C., Rodriguez, H., dos Santos, J. C., Fanucchi, S., Okabe, J., Kaipananickal, H., van Puffelen, J. H., Helder, L., Noz, M. P., Matzaraki, V., Li, Y., de Bree, L. C. J., Koeken, V. A. C. M., Moorlag, S. J. C. F. M., Mourits, V. P., Domínguez-Andrés, J., Oosting, M., ... Riksen, N. P. (2020). The Set7 Lysine Methyltransferase Regulates Plasticity in Oxidative Phosphorylation Necessary for Trained Immunity Induced by β-Glucan. Cell Reports, 31(3), [107548]. https://doi.org/10.1016/j.celrep.2020.107548 Go to publication entry in University of Southern Denmark's Research Portal Terms of use This work is brought to you by the University of Southern Denmark. Unless otherwise specified it has been shared according to the terms for self-archiving.
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