Human SETD7 / SET9 - Purified

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SA6049X Human SETD7 / SET9 - Purified

Alternate names: H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N- methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain- containing protein 7, SET7, SET7/9 Quantity: 0.5 mg Concentration: 1.0 mg/ml (determined by Bradford assay) Background: SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino- terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET 7/9, unlike most other SET proteins, is exclusively a mono-methylase. Uniprot ID: Q8WTS6 NCBI: NP_085151.1 GeneID: 80854 Species: Human Source: E. coli Format: State: Liquid Purity: >95% by SDS-PAGE Buffer System: 50 mM Tris-HCl buffer (pH7.5) 0.2M NaCl, 5mM DTT, 20% glycerol Description: Recombinant human SET7/9, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. AA Sequence: MDSDDEMVEE AVEGHLDDDG LPHGFCTVTY SSTDRFEGNF VHGEKNGRGK FFFFDGSTLE GYYVDDALQG QGVYTYEDGG VLQGTYVDGE LNGPAQEYDT DGRLIFKGQY KDNIRHGVCW IYYPDGGSLV GEVNEDGEMT GEKIAYVYPD ERTALYGKFI DGEMIEGKLA TLMSTEEGRP HFELMPGNSV YHFDKSTSSC ISTNALLPDP YESERVYVAE SLISSAGEGL FSKVAVGPNT VMSFYNGVRI THQEVDSRDW ALNGNTLSLD EETVIDVPEP YNHVSKYCAS LGHKANHSFT PNCIYDMFVH PRFGPIKCIR TLRAVEADEE LTVAYGYDHS PPGKSGPEAP EWYQVELKAF QATQQK Molecular weight: 40.7 kDa (366 amino acids) Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Bing xiao. et al(2003) Nature 421(50)652-656. 2. Taewoo Kwon. et al(2003) EMBO 22(2) 292-303.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170928 1 / 2 SA6049X: Human SETD7 / SET9 - Purified

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For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170928 2 / 2