Assessment of Bioactivity of Selected Plants Against

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Assessment of Bioactivity of Selected Plants Against ASSESSMENT OF BIOACTIVITY OF SELECTED PLANTS AGAINST PESTS AND MICROBES FROM AGRO-PASTORAL COMMUNITIES IN MBULU DISTRICT, TANZANIA MICHAEL QWARSE THESIS SUBMITTED IN FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTER OF SCIENCE IN CHEMISTRY OF THE OPEN UNIVERSTY OF TANZANIA 2015 ii CERTIFICATION The undersigned certify that he has read and hereby recommend for acceptance by the Open University of Tanzania, a thesis titled “Assessment of Bioactivity of Se- lected Plants against Pests and Microbes from Agro-pastoral Communities in Mbulu District, Tanzania” in fulfilments of the requirements for the degree of Mas- ter of Science (Chemistry) by Thesis of the Open University of Tanzania. ____________________________ Dr. Matobola J. Mihale (Supervisor) ____________________________ Date ____________________________ Dr. Joseph Sempombe (Supervisor) ____________________________ Date iii COPYRIGHT This dissertation is protected under the Berne Convention, the copyright Act of 1999 and other international and national enactments of intellectual property. No part of this dissertation may be reproduced, stored in any retrieval system or transmitted in any form by any means, electronic, mechanical, photocopying, recording or other- wise without acknowledging the author or the Open University of Tanzania in that behalf. iv DECLARATION I, Michael Qwarse, do hereby declare that this thesis is my own original work and that it has not been presented and will not be presented to any other University for a similar or any other degree award. ____________________________ Signature ____________________________ Date v DEDICATION This work is dedicated to my parents Xwatsal Margwet and Maria D. Deeng’w, my wife Antonia Onna and my childrens Christopher Onna and Christopher M. Qwarse for always being there for me all the time. vi ACKNOWLEDGEMENTS I have no words to thanks God Almighty who bestow me this opportunity to com- plete my study. I wish to express my profound gratitude to all who read the thesis and in particular my supervisors, Dr. Matobola J. Mihale and Dr. Joseph Sempombe for introducing me to this area and providing excellent scientific and personal advice, guidance, critical comments, suggestions and encouragement in this study. They al- ways supported me, had time for questions and discussions. I acknowledge the logi- cal and critical criticism, comments, suggestions and encouragement provided by Professor Veronica Mugoyela, Dr. Leonia Henry, and Dr. Fortunatus Sungwa. I sincerely acknowledge the financial support I got from the Commission for Science and Technology. I am highly indebted to the staff of Faculty of Science and Envi- ronmental Studies, Open University of Tanzania (OUT), School of Pharmacy and Institute of Traditional Medicine, Muhimbili University of Health and Allied Scienc- es for their logistic and moral support. I wish to express my sincere appreciation to beloved mum, Mrs Antonia Onna, My children, Christopher Michael and Christopher Onna for their support and encour- agements during my studies. I would like to express my gratitude to my colleagues at MUHAS: Nelson Masota, Pius Ipagala and my friends all over Tanzania amongst others: J. Siayi, Paschal P. Onna, Simon Vitalis, Reginald Barhe, Bahati Bryson, Roger, and Zaituni Msegwa for their social and moral support. vii ABSTRACT This study intended to have an ethnobotanical inventory of medicinal and pesticidal plant species and their bioactivities and toxicity against selected bacteria, fungi and storage pests. Bioactivities (antimicrobial and antioxidant activities) against disease causing organisms, toxicity against brine shrmps, first filial generation (F1) progeny, feeding deterrence and repellence against selected storage pests and quality grains evaluation as well as phytochemicals profiles were determined using standard proce- dures. A total of 66 dentifies plant species mainly from Minosoidaceae, Solanaceae, and Euphobiaceae families were used for pesticidal and medicinal purposes with oral route as the main route of administration and leaves as commonly used plant parts. Antibacterial activities of selected plants were between 25% and 75% of the activity of gentamycin sactivity. Antifungal activities of selected plant species varied from 0% to 55 % of the fluconazole activity. Antioxidant activity of Phytolacca dodecan- dra roots measured by percent absorbance was 90% as compared to 98% of the Bu- tylated Hydroxy Toluene (BHT) activity. Brine shrimp cytotoxicities ranges of P. dodecandra, (LC50 = 4.6 – 34.7 µg/mL), Cynoglossum geometrium (LC50 = 71.09 µg/mL) and Ocimum filamentosum (LC50 = 28.08 µg/mL) are indicate potential cyto- toxicity of plants. P. dodecandra leaf extracts killed 98% of Sitophilus zeamais and 99% of Tribollium castaneum at concentration of 150 mg/mL on day 3. Similarly, P. dodecandra leaf extracts reduced grain damage to 0% and had moderate repellence of 57% and 66% to Sitophilus zeamais and Tribollium castaneum, respectively. Little effects of leaf extracts on the grain quality (seed damage, colour and odour) was ob- served. Seed viability decreased to about 30% after 90 days. Isolation and structure elucidation of bioactive ingredients in selected plant species is recommended. viii TABLE OF CONTENTS CERTIFICATION .......................................................................................................... ii COPYRIGHT ................................................................................................................. iii DECLARATION ............................................................................................................ iv DEDICATION ................................................................................................................. v ACKNOWLEDGEMENTS ........................................................................................... vi ABSTRACT ................................................................................................................... vii TABLE OF CONTENTS ............................................................................................. viii LIST OF TABLES ...................................................................................................... xvii CHAPTER ONE ............................................................................................................. 1 GENERAL INTRODUCTION ...................................................................................... 1 1.1 Background of the Problem ................................................................................................ 1 1.2 Statement of the Problem .................................................................................................... 3 1.3 Objectives ................................................................................................................................ 5 1.3.1 General Objective ............................................................................................... 5 1.3.2 Specific Objectives ............................................................................................. 5 1.4 Research Questions ............................................................................................................... 5 1.5 Significance of the Study .................................................................................................... 6 CHAPTER TWO ............................................................................................................ 8 LITERATURE REVIEW ............................................................................................... 8 2.1 Plants as Sources of Bioactive Natural Products .......................................................... 8 2.2 Global Uses of Biomedicines and Biopesticides .......................................................... 9 2.2.1 Botanical Biomedicines in Tanzania ............................................................................. 10 2.2.2 Botanical Pesticides in Tanzania ...................................................................... 11 ix 2.3 Ethno-botanical Uses of Selected Plant Species ............................................... 14 2.3.1 Gymnema Sylverstre ......................................................................................... 14 2.3.1.1 Plant Description and Distribution.................................................................... 14 2.3.1.2 Traditional Uses of G. sylverstre ...................................................................... 15 2.3.2 Phytolacca dodecandra...................................................................................... 15 2.3.2.1 Plant Description and Distribution.................................................................... 15 2.3.2.2 Traditional Uses of P. dodecandra .................................................................... 16 2.3.3 Cynoglossum geometrium Bak and Wright ...................................................... 17 2.3.3.1 Plant Description and Distribution.................................................................... 17 2.3.3.2 Traditional Uses of C. geometrium ................................................................... 18 2.3.4 Leonotis Nepetifolia.......................................................................................... 18 2.3.4.1 Plant Description and Distribution.................................................................... 18 2.3.4.2 Traditional Uses of L. neptifolia ......................................................................
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