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Guidelines for the Conduct of Tests For E TG/162/4 INTERNATIONALUNION UNIONINTERNATIONALE INTERNATIONALER UNIÓNINTERNACIONAL FORTHEPROTECTION POURLAPROTECTION VERBANDZUMSCHUTZ PARALAPROTECCIÓN OFNEWVARIETIESOF DESOBTENTIONS VONPFLANZEN - DELASOB TENCIONES PLANTS VÉGÉTALES ZÜCHTUNGEN VEGETALES GUIDELINES FORTHECONDUCTOFTESTS FORDISTINCTNESS,UNIFORMITYANDSTABILITY GARLIC (Alliumsativum L.) GENEVA 2001 Copiesofthisdocumentareavailableonrequestatthepriceof10Swissfra ncseach, includingsurfacemail,fromtheOfficeofUPOV,34,chemindesColombettes,P.O.Box18, 1211Geneva20,Switzerland This document or parts of it may be reproduced, translated and published without obtainingthespecificconsentofUPOV,providedthatthesourceisacknowledged. ******* E TG/162/4 ORIGINAL: English DATE : 2001-04-04 INTERNATIONALUNION UNIONINTERNATIONALE INTERNAT IONALER UNIÓNINTERNACIONAL FORTHEPROTECTION POURLAPROTECTION VERBANDZUMSCHUTZ PARALAPROTECCIÓN OFNEWVARIETIESOF DESOBTENTIONS VONPFLANZEN - DELASOBTENCIONES PLANTS VÉGÉTALES ZÜCHTUNGEN VEGETALES GUIDELINES FORTHECONDUCTOFTESTS FORDISTINCTNESS,UNIFORMITYANDSTABILITY GARLIC (Alliumsativum L.) TheseGuidelinesshouldbereadinconjunctionwithdocumentTG/1/2, whichcontainsexplanatorynotesonthegeneralprinciplesonwhichthe Guidelineshavebeenestablished. TG/162/4 Garlic,2001 -04 -04 -2- TABLEOFCONTENTS PAGE I. SubjectoftheseGuidelines ......................................................................... 3 II. Mater ialRequired ....................................................................................... 3 III. ConductofTests ......................................................................................... 3 IV. MethodsandObservations .......................................................................... 4 V. GroupingofVarieties .................................................................................. 4 VI. CharacteristicsandSymbols ....................................................................... 4 VII. TableofCharacteristics .............................................................................. 5 VIII. ExplanationsontheTableofCharacteri stics .............................................. 12 IX. Literature ..................................................................................................... 16 X. TechnicalQuestionnaire ............................................................................. 17 TG/162/4 Garlic,2001 -04 -04 -3- I. SubjectoftheseGuidelines These Test Guidelines apply to all vegetatively propagated varieties of Allium sativum L. II. MaterialRequired 1. Thecompetentauthoritiesdec idewhen,whereandinwhatquantityandqualitytheseed requiredfortestingthevarietyistobedelivered.ApplicantssubmittingmaterialfromaState otherthanthatinwhichthetestingtakesplacemustmakesurethatallcustomsformalitiesare compliedwith.Theminimumquantityofplantmaterialtobesuppliedbytheapplicantin oneorseveralsamplesshouldbe: 50bulbs. 2. The plant material should at least meet the minimum requirements for sprouting capacity,moisturecontentandpurityformarketingplantmaterialinthecountryinwhichthe applicationismade.Itmustbeingoodsanitaryconditionandfreefromvirus,inparticular fromleekyellowstripevirusandonionyellowdwarfvirus. 3. The plant material must not have undergone any treatment unless the competent authoritiesalloworrequestsuchtreatment.Ifithasbeentreated,fulldetailsofthetreatment mustbegiven. III. ConductofTests 1. Theminimumdurationoftestsshouldnormallybetwoindependentgrowingcycle s. 2. Thetestsshouldnormallybeconductedatoneplace.Ifanyimportantcharacteristicof thevarietycannotbeseenatthatplace,thevarietymaybetestedatanadditionalplace. 3. Thetestsshouldbecarriedoutunderconditionsensuringnormal growth.Thesizeof theplotsshouldbesuchthatplantsorpartsofplantsmayberemovedformeasurementand counting without prejudice to the observations which must be made up to the end of the growingperiod.Asaminimum,eachtestshouldinclude atotalof100 plantswhichshould bedividedbetweentwoormorereplicates.Separateplotsforobservationandformeasuring canonlybeusediftheyhavebeensubjecttosimilarenvironmentalconditions. 4. Because of the effect of conditions of stora ge of bulbs on the expression of characteristics, comparison of varieties should be made only on material which has been propagated and stored under the same temperature and humidity conditions (e.g. 15oC to 18oC). 5. Additionaltestsforspecialpurposes maybeestablished. TG/162/4 Garlic,2001 -04 -04 -4- IV. MethodsandObservations 1. Unless otherwise indicated, all observations determined by measurement or counting shouldbemadeon30 plantsorpartstakenfromeachof30 plants. 2. For the assessment of uniformity a population standard of 1% with an acceptance probabilityofatleast95%shouldbeapplied.Inthecaseofasamplesizeof100 plantsthe maximumnumberofoff -typesallowedwouldbe3. 3. Allobservationsontheleaf,foliageandfloweringstemshouldbemadebeforefoliage fall -over. 4. Allobservationsonthebulbsshouldbemadeonbulbsharvestedinthetrial. V. GroupingofVarieties 1. Thecollectionofvarietiestobegrownshouldbedividedintogroupstofacilitatethe assessmentofdistinctness.Ch aracteristicswhicharesuitableforgroupingpurposesarethose whichareknownfromexperiencenottovary,ortovaryonlyslightly,withinavariety.Their variousstatesofexpressionshouldbefairlyevenlydistributedthroughoutthecollection. 2. Itisrecommendedthatthecompetentauthoritiesusethefollowingcharacteristicsfor groupingvarieties: (a) Pseudostem:floweringstem(characteristic10); (b) Clove:colorofscale(characteristic29); (c) Timeofharvestmaturity(characteristic33); (d) Endofdormancyofcloveinbulb(characteristic34). VI. CharacteristicsandSymbols 1. To assess distinctness, uniformity and stability, the characteristics and their states as givenintheTableofCharacteristicsshouldbeused. 2. Notes(numbers),forthe purposesofelectronicdataprocessing,aregivenoppositethe statesofexpressionforeachcharacteristic. 3. Legend: (*) Characteristicsthatshouldbeusedonallvarietiesineverygrowingcycleoverwhich theexaminationsaremadeandalwaysbein cludedinthevarietydescriptions,except when the state of expression of a preceding characteristic or regional environmental conditionsrenderthisimpossible. (+) SeeExplanationsontheTableofCharacteristicsinChapterVIII. TG/162/4 Garlic/Ail/Knoblauch/Ajo,2001 -04 -04 -5- VII. TableofCharac teristics/Tableaudescaractères/Merkmalstabelle/Tabladecaracteres ExampleVarieties English français deutsch español Exemples Note/ Beispielssorten Nota Variedadesejemplo 1. Foliage:density Feuillage:densité Laub:Dichte Follaje:densida d (*) loose lâche locker laxo Ramses 3 medium moyen mittel medio Printanor 5 dense dense dicht denso Germidour 7 2. Foliage:attitude Feuillage:port Laub:Haltung Follaje:porte (*) (+) erect dressé aufrecht erecto Jolimont 1 erectto dresséà aufrechtbis erectoa Printanor 2 semi -ere ct demi- dressé halbaufrecht semierecto semi -erect demi- dressé halbaufrecht semierecto Jardinor 3 3. Leaf:greencolor Feuille:couleurverte Blatt:Grünfärbung Hoja:colorverde (*) light claire hell claro 3 medium moyenne mittel medio Messidrome 5 dark foncée dunkel oscuro Germidour 7 4. Leaf:waxiness Feuille:pruine Blatt:Wachsschicht Hoja:cerosidad absentorveryweak nulleoutrèsfaible fehlendodersehrgering ausenteomuydébil 1 wea k faible gering débil Sprint 3 medium moyenne mittel media Messidrome 5 strong forte stark fuerte Germidour,Moratop 7 verystrong trèsforte sehrstark muyfuerte Gayant,Printanor 9 5. Leaf:length Feuille:longueur Blatt:Länge Hoja:longitud (*) (longestleaf) (feuillela pluslongue) (längstesBlatt) (hojamáslarga) short courte kurz corta Jardinor 3 medium moyenne mittel media Moraluz,Morasur 5 long longue lang larga Sultop 7 TG/162/4 Garlic/Ail/Knoblauch/Ajo,2001 -04 -04 -6- ExampleVarieties English français deutsch español Exemples Note/ Beispielssorten Nota Variedadesejemplo 6. Leaf:width Feuille:largeur Blatt:Breite Hoja:anchura (*) (asfor5) (commepour 5) (wieunter5) (comopara5) narrow étroite schmal estrecha Ramses 3 medium moyenne mittel media Printanor 5 broad large breit ancha Germidour 7 7. Leaf:shapeincross Feuille:formeen Blatt:Formim Hoja:formadela (*) section sectiontransver sale Querschnitt seccióntransversal stronglyconcave fortementconcave starkkonkav fuertementecóncava 1 slightlyconcave légèrementconcave leichtkonkav ligeramentecóncava Ramses 2 flat plane flach plana Germi dour 3 8. Pseudostem:intensity Pseudotige:intensité Pseudostamm: Pseudotallo: (*) ofanthocyanin delapigmentation Intensitätder intensidaddela colorationatbase anthocyaniqueàla Anthocyanfärbungan pigmentación base derBasis antociánicaenlab ase absentorveryweak absenteoutrèsfaible fehlendodersehrgering ausenteomuydébil Printanor 1 weak faible gering débil Messidrome 3 medium moyenne mittel media 5 strong forte stark fuerte Germidour 7 verystrong trèsforte sehrstar k muyfuerte 9 9. Pseudostem:widthof Pseudotige:largeurde Pseudostamm:Breite Pseudotallo:anchura (*) thebase labase derBasis delabase narrow étroite schmal estrecha Ramses 3 medium moyenne mittel media Printanor 5 broad large breit anc ha Germidour 7 10. Pseudostem:flowering Pseudotige:tige Pseudostamm: Pseudotallo:tallo (*) stem florifère Blütenstandstiel floral absent absente fehlend ausente Germidour 1 present présente vorhanden presente RosedeLautrec 9 TG/162/4 Garlic/Ail/Knoblauch/Ajo,2001 -04 -04 -7- ExampleVarieties English français deutsch español Exemples Note/ Beispielssorten Nota Variedadesejemplo 11. Flower ingstem: Tigeflorale: Blütenstandstiel: Tallofloral: (*) curvature courbure Krümmung curvatura (+) absent absente fehlend ausente Morasur,Sultop 1 present présente vorhanden presente
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