DOCSLIB.ORG

SCP1, a Major Protein Component of Synaptonemal Complexes of the Rat

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
SCP1, a Major Protein Component of Synaptonemal Complexes of the Rat SCP1,a majo rprotei n componento f synaptonemal complexes ofth era t SCPl, eenbelangrijk e eiwit-component van synaptonemale complexenva nd era t Promotor: dr.C .Heytin g hoogleraar ind emoleculair ee n celgenetica Ralph Leo Johan Meuwissen SCP1,a majo rprotei n componento f synaptonemal complexes ofth era t Proefschrift terverkrijgin g van degraa d van doctor op gezagva n derecto r magnificus van deLandbouwuniversitei t Wageningen, dr.CM . Karssen, inhe t openbaar te verdedigen opdinsda g4 februari 1997 desnamiddag s tehal ftwe e ind eAula . CIP-DATAKONINKLIJK E BIBLIOTHEEK,DE NHAA G Meuwissen, Ralph LeoJoha n SCP1, amajo r protein component of synaptonemal complexes ofth era t/ R.L.J.Meuwissen . - [S.l. :s.n.] .- 111. Thesis Wageningen. -Wit href . With summary inDutc h and Spanish. ISBN 90-5485-649-1 Subject headings:meiosi s / SCP1/ synaptonemal complex/ Rattus rattus norvegicus Theresearc h described inthi sthesi swa sfunde d byth eMedica l Science Section (GMW)whic h issubsidise d byth eNetherland s Organization for Scientific Research (NWO). NMoS^Ol.fc^S Stellingen 1. SCPl isee nhoofdcomponen t vand etransversal e filamenten vanhe t synaptonemale complex. dit proefschrift 2. Onderzoek waarbij polyklonale antisera,opgewek ttege noverlappend eS C eiwitfragmenten, wordengebruik tvoo rimmunogou d labeling experimenten metS C preparaten van gespreide spermatocyten, leverenonbetrouwbar eresultate n opdi enie t zonder meergebruik t kunnen wordenvoo rd elokalisati e bepaling vandi teiwi t inhe tSC . Dobsone tal .J. Cell Sci. 107 :2749-276 0 dit proefschrift 3. Deafzonderlijk e SCeiwitte n vanS. cerevisaee nzoogdiere nkunne nee ngroter e structurelee nfunctionel e overeenkomstvertone nda nme no pgron dva nnu ngelijkeni s ind e primaire aminozuurvolgorde zouverwachten . 4. Devraa g ofee nintact e SCstructuu r verantwoordelijk isvoo rhe tverschijnse l van "crossover interferentie" kanbete ri nee nredl danee nziplredl achtergrond inS. cerevisae worden bestudeerd. Storlazzi etal .Proc. Natl. Acad. Sci.USA 93: 9043-9048 5. Hetafdwinge n van sluitingstijden doorhe t gebruikva n semi-professionele bewakingsapparatuur inee nuniversitai r laboratorium leidta fva nd eprimair edoelstellin g als faciliteit voorhe t optimaal kunnenuitvoere nva nwetenschappelij k onderzoek. Deze maatregel werkthierdoo r volledigcontra-productief . 6. Machtswellust isvaa kgebaseer d oppur eangst . 7. Nietallee nJezu sdroe gzij n eigenkruis . 8. Deopmerkin gva nwesters esociaal-historic i datAfrik a een "verlorencontinent "zo uzij n methe too g opstabiel ewelvaartsgroei ,gaa tvoorbi j aanhe t feit datd edeplorabel e economischetoestan d inmeni gA&ikaans estaa tjuis t ind ehan dword tgewerk tdoo rhe t actiefsteune n vanincorapetent ee ncorrupt eregime sdoo rwesters e regeringen. 9. Eent ehoo gaandee iva nspelletje s enloterij-programma' s inhe taanbo dva nd e commercieletelevisi eontaard ta lgau wi nplee-TV . 10. DNAhebje voor 't leven. 11. Eenslapp ehaa nkraai tnie tmeer . 12. Eendispuu t brengtweini gvrienden . 13. Intellectuelen zijn vaak tesli m voorhe topiosse n vaneenvoudig eproblemen . Steilingenbehoren d bijhe tproefschrif t 'SCP1,a majo r componento fsynaptonema l complexes ofth erat ' vanRalp hMeuwisse n Wageningen,4 februari 1997 Voormij n ouders Contents Chapter1 Introduction, 3 Chapter2 Acoiled-coi lrelate dprotei nspecifi c for synapsed regions of meioticprophas e chromosomes,2 5 Chapter3 Human synaptonemal complexprotei n 1 (SCP1): isolation and chracterization of the cDNAan d chromosomal localization of thegene ,3 7 Chapter4 Bindingo fDN Ab y SCP1,a majo r componento f synaptonemal complexes, 53 Chapter5 Organization of SCP1protei n moleculeswithi n synaptonemal complexes of therat , 79 Chapter6 GeneralDiscussion , 101 Summary 115 Resumen 119 Samenvatting 123 Dankwoord 127 CurriculumVita e 129 CHAPTER 1 Introduction INTRODUCTION Meiosis isa nessentia l process inth e sexual reproduction cycle ofeukaryotes . Itbring s about the transition from diploid to haploid generations of cells. At fertilization, the diploid state isrestore d whentw ohaploi d gametes fuse to form adiploi d zygote. Meiosis consists of two successive nuclear divisions, meiosis I and II, which follow a single round of DNA replication. After premeiotic S-phase, during the prophase of meiosis I, a series of characteristic chromatin rearrangements take place by which homologous chromosomes condense and pair, and the non-sister chromatids of homologous chromosomes (homologues) recombine with each other (von Wettstein et al, 1984; Loidl, 1994; Griffiths et al, 1996). These events are followed by segregation ofth ehomologue s (Figure 1). The segregation of the homologues causes the reduction of the chromosome complement from diploid to haploid: meiosis I is therefore called a reductional division. Meiosis II, in contrast, is an equational division because the ploidy level is maintained: the two sister chromatids of each chromosome are then separated, as in a mitotic division (Fig.l). The characteristic aspects of chromosome behaviour in meiosis are summarized in Table I, which shows that many meiosis-specific events take place during meiotic prophase. These events, namely the pairing and recombination of homologous chromosomes, are accompanied by the assembly and disassembly of meiosis-specific structures, the synaptonemal complexes (SCs) (Moses, 1969). TableI Chromosome behaviour during eukaryotic cell divisions Meiosis 1 Meiosis II Mitosis Pairing of homologous Usually no pairing between chromosomes homologous chromosomes High frequency of recombination, Low frequency of recombination, preferably between non-sister preferably between sister chromatids chromatids Sister chromatid cohesion until no cohesion cohesion until anaphase anaphaseI The two sister-centromeres act as a two sister-centromeres act two sister-centromeres act unit independently from each other and independently from each other and are separated are separated 1)n.a. , not applicable DNA replication DNA replication X pairingo f homologous chromosomes duplicated chromosomes line up individually on thespindl e homologous pairso f duplicatedchromosome s line upo nth espindl e ivision >^\. Figure 1. The process of meiosis compared to mitosis. Only one set of homologous chromosomes is shown. The pairing of the duplicated homologous chromosomes isuniqu e to meiosis. During meiosis, two nuclear divisions are needed to produce haploid gametes. Each diploid cell entering meiosis thus produces four haploid cells (from Alberts et al., 1989). Synaptonemal complexes (SCs) The SC is aproteinaceous , zipper-like structure which holds the homologues in close apposition along their entire length during meiotic prophase (von Wettstein et al, 1984) (Figure 2a).A t the beginning ofmeioti c prophase, after premeiotic S-phase, the two sister chromatids develop a single proteinaceous axis, called axial element. As meiotic prophase proceeds, these axial elements are connected along their entire length by numerous thin, transverse filaments (TFs). Between the axial elements resides a third longitudinal structure, referred to as the central element (CE). The two axial elements together with the CE make up the so-called tripartite SC structure. Within the context of the SC,th e axial elements are also called lateral elements (LEs) of the SC. Homologues are called synapsed if they are connected by the tripartite structure. The tripartite SC structure is conserved among sexually reproducing eukaryotes (Dresser and Giroux, 1988;Schmeke l etal, 1993;Schmeke l and Daneholt, 1995), although there is much variation in both CE structure and LE shape (von Wettstein etal, 1984). A schematic overview of the (dis)assembly of SCs and the concurrent chromosome rearrangements is shown in Figure 2a and 2b. The successive stages of meiotic prophase are defined by the stages of SC (dis)assembly (Figure 2a): during leptotene, a single axial element is formed between the sister chromatids; during zygotene, the axial elements of homologues are connected by TFs, and the CE appears in synapsed regions. During pachytene, the homologues are synapsed from telomere to telomere. In diplotene, the tripartite structure starts to disassemble; in mammals the TFs disappear first (von Wettstein et al, 1984). At the end of diplotene and the onset of diakinesis, the LEs have disappeared and the two scaffolds of the individual sister chromatids (shown in Figure 2b) become discernible as the now recombined chromosomes condense further. The chiasmata become visible as the results of reciprocal exchange between twonon-siste r chromatids ofhomologues . In metaphase I, the nuclear envelope disappears and the chromosomes condense further while the centromeres are attached to the spindle and chromosome pairs (bivalents) orientthemselve s inpreparatio n ofchromosom e disjunction. Recombination nodules During the assembly of SCs, electron-dense spherical or ellipsoidal structures with a diameter of 100-200n mar e formed, the so-called recombination nodules (RNs) a) Leptotene Pachytene I I I I 14 ^Mii, I Diplotene Interphase Zygotene followed by diakenesis > b) Leptotene/zygotene MetaphaseI Nuclear envelope Figure 2. a) A schematic figure showing the successive stages of meiotic prophase as defined on the basis of morphological changes of the SC: leptotene (proteinaceous axes start to form along the homologous chromosomes), zygotene (start of synapsis of the homologues), pachytene (synapsis is completed and the SC extends from telomere to telomere), diplotene (SC disassembles) and
Load more

Recommended publications
  • The Centenary of Janssens's Chiasmatype Theory
    PERSPECTIVES The Centenary of Janssens’s Chiasmatype Theory Romain Koszul,*,†,1 Matthew Meselson,‡ Karine Van Doninck,§ Jean Vandenhaute,** and Denise Zickler††,1 *Institut Pasteur, Group Spatial Regulation of Genomes, Department of Genomes and Genetics, F-75015 Paris, France, †2 Centre National de la Recherche Scientifique, Unité Mixte de Recherche (UMR) 3525, F-75015 Paris, France, ‡Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, §Université de Namur, Facultés universitaires Notre-Dame de la Paix (FUNDP), Unité de Recherche en Biologie des Organismes, B5000 Namur, Belgium, **Université de Namur, FUNDP, Unité de Recherche en Biologie Moléculaire et Département Sciences, Philosophies et Sociétés, B5000 Namur, Belgium, and ††Institut de Génétique et Microbiologie, UMR 8621, Université Paris-Sud, 91405 Orsay, France ABSTRACT The segregation and random assortment of characters observed by Mendel have their basis in the behavior of chromosomes in meiosis. But showing this actually to be the case requires a correct understanding of the meiotic behavior of chromosomes. This was achieved only gradually, over several decades, with much dispute and confusion along the way. One crucial step in the understanding of meiosis was provided in 1909 by Frans Alfons Janssens who published in La Cellule an article entitled “La théorie de la Chiasmatypie. Nouvelle interprétation des cinèses de maturation,” which contains the first description of the chiasma structure. He observed that, of the four chromatids present at the connection sites (chiasmata sites) at diplotene or anaphase of the first meiotic division, two crossed each other and two did not. He therefore postulated that the maternal and paternal chromatids that crossed penetrated the other until they broke and rejoined in maternal and paternal segments new ways; the other two chromatids remained free and thus intact.
    [Show full text]
  • Meiosis in Haploid Rye: Extensive Synapsis and Low Chiasma Frequency
    Heredity 73 (1994 580—588 Received 8 February 1994 Genetical Society of Great Britain Meiosis inhaploidrye: extensive synapsis and low chiasma frequency J. L. SANTOS*, M. M. JIMENEZ & M. D1EZ Department of Genetics, Faculty of Biology, Universidad Comp/utense de Madrid, 28040 Madrid, Spain Extensivesynaptonemal complex formation was found at prophase I in whole mount spread preparations of a spontaneous haploid rye, Secale cereale, with values of up to 87.8 per cent of the chromosome complement synapsed. Pairing-partner switches were frequent, giving rise to multiple associations in which all or most of the chromosomes were involved. However, the distribution of synaptonemal complex stretches suggests that synapsis does not occur at random. The frequency of multivalents and the mean frequency of bonded arms at metaphase I were 0.03 and 0.39, respectively. Associations between chromosome arms without heterochromatin were more frequent than between the remaining arms. The observation of recombinant chromosomes for telomeric C-bands at anaphase I indicates that metaphase I bonds are true chiasmata. The correspondence between the location of pairing initiation sites and chiasmata indicates that early synapsis could be confined to homologous regions. Keywords:C-bands,haploid rye, metaphase I bonds, rye, Secale cereale, synapsis. Introduction the case in different monoploids of rye (Levan, 1942; Neijzing, 1982; deJong eta!., 1991). Individualswith chromosome numbers corresponding Pachytene observations by light microscopy to those of the gametes of their species are designated revealed occasional interchromosomal and intra- by the general term 'haploids'. An individual with the chromosomal pairing in monoploids of rice (Chu, gametic chromosome number derived from a diploid 1967), tomato (Ecochard et al., 1969), maize (Ting, species is called 'monoploid' or simply 'haploid', 1966; Ford, 1970; Weber & Alexander, 1972) and whereas the term 'polyhaploid' is used when it is pro- barley (Sadasivaiah & Kasha, 1971, 1973).
    [Show full text]
  • Meiosis, Recombination, and Interference
    Meiosis, recombination, and interference Karl W Broman Department of Biostatistics Johns Hopkins University Baltimore, Maryland, USA www.biostat.jhsph.edu/˜kbroman Outline Mitosis and meiosis Chiasmata, crossovers Genetic distance Genetic markers, recombination Chromatid and chiasma interference Mather’s formula The count-location model ¢ The gamma model and the ¡ model Data: humans and mice Mitosis: ordinary cell division Chromosomes Chr's pull apart duplicate and cell divides Chromosomes line up Meiosis: production of sex cells Chr's pull apart and cells divide Chr's exchange material and cell divides Chromosomes duplicate Chromosomes pair up The exchange process Vocabulary Four-strand bundle Meiotic products Sister chromatids Non-sister chromatids Chiasma, chiasmata Crossovers Obligate chiasma Genetic distance Two points are d Morgans apart if the average number of crossovers per meiotic product in the intervening interval is d. Usual units: centiMorgan (cM); 100 cM = 1 Morgan ¡ Genetic distance Physical distance The intensity of the crossover process varies by Sex Individual Chromosome Position on chromosome Temperature But we don’t observe crossovers Crossover Crossovers generally not process observeable Marker We instead observe the origin data of DNA at marker loci. odd no. crossovers = recombination event even no. crossovers = no recombination Recombination fraction = Pr(recombination event in interval) Microsatellite markers aka Short Tandem Repeat Polymorphisms (STRPs) GA T AGA T A GA T A CT A TCT A T CT A T Tandem repeat of something like GATA at a specific position in the genome. − Number of repeats varies Use PCR to “amplify” region Use gel electrophoresis to determine length of region + Map functions Connect genetic distance (average no.
    [Show full text]
  • Mycelial Fungi Are Typically Multinucleate (Many Nuclei in a Common
    Biology of Fungi Lecture 9: Fungal Genetics, Molecular Genetics, and Genomics Q Mycelial fungi are typically multinucleate (many nuclei in a common cytoplasm) and reap both the benefits of being haploid and a “functional diploid” Q Not so for solely diploid organisms (e.g., Oomycota) and it also different for organisms like S. cerevisiae which can have either a persistent haploid or diploid state u Nonsexual variation: heterokaryosis Q Heterokaryons have two or more genetically different nuclei in a common cytoplasm (as opposed to homokaryons) Q Heterokaryons produced in two ways: l Mutation of a wild-type nucleus l Fusion of two genetically different strains Q Ratio of genetically different nuclei can vary depending upon selection pressures, e.g., Jinks test involving Penicillium cyclopium (see Table 9.3 in Deacon) Q Heterokaryons do break down or segregate into homokaryons by one of two mechanisms l Uninucleate spore production l Branch contains a single nuclear type u Nonsexual variation: parasexuality Q Discovered by Pontecorvo in studying heterokaryosis Q He observed that a heterokaryon having nuclear condition Ab and aB, produced not only parental types (Ab and aB), but also recombinant types, AB and ab. Q Further study of this phenomenon led Pontecorvo to propose a parasexual cycle consisting of three stages: l Diploidization l Mitotic chiasma formation l Haploidization Q Parasexuality in relatively rare and is not a regular cycle as is sexual reproduction u Sexual variation Q Major mechanism for producing recombinants Q Different
    [Show full text]
  • The Biological Bulletin
    THE BIOLOGICAL BULLETIN PUBLISHED BY THE MARINE BIOLOGICAL LABORATORY Editorial Board E. G. CONKLIN, Princeton University CARL R. MOORE, University of Chicago E. N. HARVEY, Princeton University GEORGE T. MOORE, Missouri Botanical Garden SELIG HECHT, Columbia University T jj MORGAN, California Institute of Technology LEIGH HOADLEY, Harvard University Q H pARKER Harvard University L. IRVING, Swarthmore College A ' C ' REDFffiLD, Harvard University M. H. JACOBS, University of Pennsylvania Columbia H. S. JENNINGS, Johns Hopkins University F. SCHRADER, University FRANK R. LILLIE, University of Chicago DOUGLAS WHITAKER, Stanford University H. B. STEINBACH, Washington University Managing Editor VOLUME 89 AUGUST TO DECEMBER, 1945 Printed and Issued by LANCASTER PRESS, Inc. PRINCE & LEMON STS. LANCASTER, PA. 11 THE BIOLOGICAL BULLETIN is issued six times a year at the Lancaster Press, Inc., Prince and Lemon Streets, Lancaster, Penn- sylvania. Subscriptions and similar matter should be addressed to The Biological Bulletin, Marine Biological Laboratory, Woods Hole, Massachusetts. Agent for Great Britain: Wheldon and Wesley, Limited, 2, 3 and 4 Arthur Street, New Oxford Street, London, W. C. 2. Single numbers, $1.75. Subscription per volume (three issues), $4.50. Communications relative to manuscripts should be sent to the Managing Editor, Marine Biological Laboratory, Woods Hole, Massachusetts, between July 1 and October 1, and to the Depart- ment of Zoology, Washington University, St. Louis, Missouri, during the remainder of the year. Entered as second-class matter May 17, 1930, at the post office at Lancaster, Pa., under the Act of August 24, 1912. LANCASTER PRESS, INC., LANCASTER, PA. CONTENTS No. 1. AUGUST, 1945 PAGE ANNUAL REPORT OF THE MARINE BIOLOGICAL LABORATORY 1 WHITING, ANNA Dominant lethality and correlated chromosome effects in Habrobracon eggs x-rayed in diplotene and in late metaphase I 61 HARVEY, ETHEL BROWNE Stratification and breaking of the Arbacia punctulata egg when cen- trifuged in single salt solutions 72 PACE, D.
    [Show full text]
  • The Relation Between Chiasmata and Crossing-Over in Diploid and Triploid
    THE I%ELATION BETWEEN CHIASMATA AND CROSSING-OVER IN DIPLOID AND TRIPLOID DROSOPHILA MELAI¥OGA~TER. BY K. MATHEI%. (John Innes Horticultu~'aZ Institution, Me,rton.) (With Three Text-figures.) I. INTI%O])UOTION. T1~E genetical observations on Drosol)hila melanogaste~" have, in the past, demonstrated severat principles of paramount importance to the cytolo- gist, some indeed being fundamental to our present theory of meiosis. Note recently work has been done on triptoid forms of D. melanogaste'r, which for its interpretation needs a fuller knowledge of the meiotic be- haviour of chromosomes than it has hitherto been possible to derive from cytological observations on Drosophila itself. At the same time, the study of meiosis in more favourable material, notably in monocotyledonous plants, has led to the phenomenon being placed on a sound theoretical basis. It is the object of this paper to apply cytological findings to the fuller interpretation and lmderstanding of some of the recent genetical work on D. melanogaste'r. For the purpose of exposition Darlington's modification of Janssens' chiasmatype theory has been adopted. On this theory ehiasma formation is conditioned by crossing-over, but similar deduc- tions, in the majority of cases, would follow from some other theories of chiasma formation and its relationship with crossing-over. Where this is not the case, special note is made of it. II. THE DIPLOID--INTERFERENCE. 3/Iost of the work on crossing-ovor in diploid D. ,melanoyaste'r has boon in connection with linkage maps, and the study of coincidence values. ]Recently, however, several experiments involving a mlmber of points scattered along a chromosome have beo~k performed, mainly in order to provide controls for a study of crossing-over in some relatively abnormal forms.
    [Show full text]
  • Genomic Consequences of Hybridization Between Rainbow and Cutthroat Trout
    Genomic Consequences of Hybridization between Rainbow and Cutthroat Trout Carl O. Ostberg A dissertation Submitted in partial fulfillment of the Requirements for the degree of Doctor of Philosophy University of Washington 2015 Reading Committee Lorenz Hauser, Chair Kerry-Ann Naish Steven Roberts Program Authorized to Offer Degree: School of Aquatic and Fishery Sciences i ©Copyright 2015 Carl O. Ostberg ii University of Washington Abstract Genomic Consequences of Hybridization between Rainbow and Cutthroat Trout Carl Olof Ostberg Chairperson of the Supervisory Committee: Lorenz Hauser, Associate Professor School of Aquatic and Fishery Sciences Introgressive hybridization creates novel gene combinations that may generate important evolutionary novelty and thus contribute to biological complexity and diversification. On the other hand, hybridization with introduced species can threaten native species, such as cutthroat trout ( Oncorhynchus clarkii ) following the introduction of rainbow trout ( O. mykiss ). While rainbow trout introgression in cutthroat trout is well documented, neither the evolutionary consequences nor conservation implications are well understood. Hybridization between rainbow and cutthroat trout occurs in the context of substantial chromosomal rearrangement, as well incompletely re-diploidized genomes. Rainbow and cutthroat trout are descended from an autopolyploid ancestor, and extensive chromosome arm rearrangements have occurred between the species following their divergence from the last common ancestor. Evidence for incomplete re- diploidization includes the occasional formation of multivalents and duplicated loci occasionally exhibit a mixture of disomic and tetrasomic inheritance. Thus, transmission genetics may be complicated by recombination between homeologs. Here, I evaluated the phenotypic and genetic consequences of introgression between rainbow trout and Yellowstone cutthroat trout ( O. clarkii bouvieri ) to provide insights into genome processes that may help iii explain how introgression affects hybrid genome evolution.
    [Show full text]
  • The Relationship Between Chiasmata and Crossing Over in Triticum Aestivumi
    THE RELATIONSHIP BETWEEN CHIASMATA AND CROSSING OVER IN TRITICUM AESTIVUMI T. K. FU* AND E. R. SEARSS Department of Agronomy, University of Missouri, Columbia, Missouri 65201 Manuscript received March 19, 1973 Revised copy received June 8, 1973 ABSTRACT Telocentrics for the p arm of chromosome 4A and the long arm of 6B were used as cytological markers for the determination of chiasma frequency. In concomitant studies of recombination, terminal segments of rye and T. um- bellulatum chromatin carrying Hp (Hairy peduncle) and Lr9 (Leaf-rust re- sistance), respectively, marked 4A and 6B. Two temperatures, 21" and 32", were used for bgth the 4A and 6B experiments.--Only one chiasma was observed in each heteromorphic bivalent. Because there was a substantial re- duction in pairing between diakinesis and metaphase I, all determinations of chiasma frequency were made at diakinesis. In the 21" experiments, agree- ment was good between genetic recombination and cytological prediction on the basis of the partial chiasmatypy hypothesis that each chiasma represents a crossover. At 32" both chiasma frequency and crossing over, but particu- larly the latter, were strongly reduced. The fewer crossovers than expected are explained in part hy stickiness of chromosomes at the high temperature, some- times resultkg in adjacent chromosomes being wrongly scored as having a chiasma, and in part by premetaphase disjunction of some recombined bivalents and subsequent independent behavior of the two resulting univalents.-Male transmission of the 4A telocentric
    [Show full text]
  • CDKG1 Protein Kinase Is Essential for Synapsis and Male Meiosis at High Ambient Temperature in Arabidopsis Thaliana
    CDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana Tao Zhenga,1,2, Candida Nibaub,1, Dylan W. Phillipsb, Glyn Jenkinsb, Susan J. Armstrongc, and John H. Doonanb,3 aJohn Innes Centre, Norwich NR4 7UH, United Kingdom; bInstitute of Biological, Environmental, and Rural Sciences, Aberystwyth University, Aberystwyth SY23 3EB, United Kingdom; and cSchool of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom Edited by David C. Baulcombe, University of Cambridge, Cambridge, United Kingdom, and approved January 3, 2014 (received for review October 9, 2013) The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines mitotic and meiotic cell cycles. Cdc28 in budding yeast has a role a clade of cyclin-dependent protein kinases related to CDK10 and in Spo11-dependent DSB formation and is necessary for SC as- CDK11, as well as to the enigmatic Ph1-related kinases that are sembly (2). CDK2 is required to prevent nonhomologous chro- implicated in controlling homeologous chromosome pairing in mosome pairing in mice (3), and the Arabidopsis homolog CDKA;1 wheat. Here we demonstrate that the CDKG1/CYCLINL complex is necessary for the meiotic process (4), perhaps through regulation is essential for synapsis and recombination during male meiosis. of retinoblastoma-related protein (Rbr) activity (5). Ph1,acomplex A transfer-DNA insertional mutation in the cdkg1 gene leads to locus containing multiple copies of CDK-like genes (6), enhances a temperature-sensitive failure of meiosis in late Zygotene/Pachy- the fidelity of chromosome pairing in hexaploid wheat (7), such tene that is associated with defective formation of the synapto- that homologous chromosomes cross over but homeologs do not nemal complex, reduced bivalent formation and crossing over, and (8).
    [Show full text]
  • Segregation in Yeast LYLE 0
    Proc. Natl. Acad. Sci. USA Vol. 93, pp. 4979-4983, May 1996 Genetics Exchanges are not equally able to enhance meiotic chromosome segregation in yeast LYLE 0. Ross*, REBECCA MAXFIELD, AND DEAN DAWSONt Department of Molecular Biology and Microbiology, Tufts University, 136 Harrison Avenue, Boston, MA 02111 Communicated by Nancy Kleckner, Harvard University, Cambridge, MA, January 18, 1996 (received for review July 1, 1995) ABSTRACT Homologous chromosomes pair, and then Crossovers do not guarantee disjunction, rather they en- migrate to opposite poles of the spindle at meiosis I. In most hance the chances that it will occur. The observation of eukaryotic organisms, reciprocal recombinations (cross- nondisjoined homologs that have experienced crossovers raises overs) between the homologs are critical to the success of this the possibility that some crossovers may have properties that process. Individuals with defects in meiotic recombination cause them to form less effective chiasmata than other cross- typically produce high levels of aneuploid gametes and exhibit overs. The experiments described here were designed to test low fertility or are sterile. The experiments described here systematically whether different crossovers were equally able were designed to test whether different crossovers are equally to enhance the fidelity of meiotic chromosome segregation. To able to contribute to the fidelity of meiotic chromosome test the ability of a chromosomal exchange to influence segregation in yeast. These experiments were performed with disjunction, it is necessary that the exchange be the only one model chromosomes with which it was possible to control and between the homologs whose segregation is being monitored. measure the distributions of meiotic crossovers in wild-type This situation is rare in yeast, because even the smallest cells.
    [Show full text]
  • Chiasma Interference and Centromere Co-Orientation in a Spontaneous Translocation Heterozygote of Euchorthippus Pulvinatus Gallicus (Acrididae; Orthoptera)
    CHROMOSOMA Chromosoma (Berl.) 78, 327-340 (1980) by Springer-Verlag 1980 Chiasma Interference and Centromere Co-orientation in a Spontaneous Translocation Heterozygote of Euchorthippus pulvinatus gallicus (Acrididae; Orthoptera) P. Arana, J.L. Santos and R. Giraldez Departamento de Gen&ica, Facultad de Biologia, Universidad Complutense, Madrid, Spain Abstract. The meiosis of an individual of the species Euchorthippuspulvinatus gallicus heterozygous for a reciprocal translocation involving chromosomes 3 and 6 has been analysed using the Giemsa C-banding technique. It is concluded that: (i) Chiasma interference in the quadrivalent seems to act only at the arm level. There is no interference across the translocation break point. No interchromosomal chiasma interference could be demonstrated. (ii) The results concerning the co-orientation of the quadrivalent suggest that the length of the chromosomal segments between two adjacent centro- meres at metaphase I is related with their orientation behaviour. Introduction From the genetic point of view, crossing-over and centromere co-orientation are the two phenomena leading to the most important events taking place during the first meiotic division, namely, genetic recombination and chromosome number reduction. Translocation heterozygotes in grasshoppers are a very favourable material for the study of some aspects of both phenomena. In several instances the analysis of interchromosomal effects in chiasma frequency and of co-orientation has been carried out using these interchange heterozygotes (Sarkar, 1955; Kayano and Nakamura 1960; Lewis and John, 1963; John and Hewitt, 1963; Hewitt and John, 1965; Hewitt, 1967; Southern, 1967b; Wise and Rickards, 1977; Sannomiya, 1978). The Giemsa C-banding technique offers the possibility of recognizing the different multivalent arms as well as the location of centromeres of such multiva- lents and thus provides a basis for an accurate analysis.
    [Show full text]
  • Competition for Chiasma Formation Between Identical and Homologous (But Not Identical) Chromosomes in Synthetic Autotetraploids of Arabidopsis Thaliana
    fpls-09-01924 January 2, 2019 Time: 18:19 # 1 ORIGINAL RESEARCH published: 09 January 2019 doi: 10.3389/fpls.2018.01924 Competition for Chiasma Formation Between Identical and Homologous (But Not Identical) Chromosomes in Synthetic Autotetraploids of Arabidopsis thaliana Pablo Parra-Nunez, Mónica Pradillo* and Juan Luis Santos Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, Madrid, Spain Polyploid organisms provide additional opportunities to study meiosis in a more complex context since more than two potential homologous chromosomes are available. When the chromosome complement of a diploid individual is duplicated, each chromosome is accompanied by one identical and two homologous chromosomes within the same nucleus. In this situation, a competition in pairing/synapsis/chiasma formation between identical and homologous (but not necessarily identical) chromosomes can occur. Several studies have been conducted in different species to address whether there Edited by: are preferences in crossover formation between identical rather than homologous Simon Gilroy, University of Wisconsin–Madison, chromosomes. In this study, multivalent and chiasma frequencies were cytologically United States analyzed in synthetic autotetraploids of Arabidopsis thaliana including the accessions Reviewed by: Col, Ler, and the Col/Ler hybrid. Fluorescence in situ hybridization was conducted to Paul Fransz, University of Amsterdam, Netherlands identify each chromosome at metaphase I. The new Col and Ler tetraploids showed high James D. Higgins, multivalent frequencies, exceeding the theoretical 66.66% expected on a simple random University of Leicester, end-pairing model, thus indicating that there are more than two autonomous synaptic United Kingdom sites per chromosome despite their small size.
    [Show full text]