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12th International Symposium on Biogeochemistry of

April 23-26, 2018 Coral Springs, Florida, USA

www.conference.ifas.ufl.edu/biogeo2018 12th International Symposium on Biogeochemistry of Wetlands

About the Symposium

Wetland biogeochemistry is dynamic and involves cycling or exchange or flux of materials between living and non-living components of an . Biogeochemistry is an interdisciplinary science which includes the study of interactive biological, geological and chemical processes regulating the fate and transport of nutrients and contaminants in soil, water and atmospheric components of an ecosystem. Biogeochemistry also provides a framework to integrate physical, chemical and biological processes functioning in an ecosystem at various spatial and temporal scales. This international symposium provides a framework for to share technical information on various topics related to coupled biogeochemical cycling of macro-elements and associated organic and inorganic contaminants. The goal is to improve our understanding of the role wetlands perform in regulating and mitigating impacts of global and sea level rise.

2 April 23-26, 2018 | Coral Springs, Florida, USA

Table of Contents

About the Symposium...... 2 Welcome Letter from Conference Chairs...... 5 Committee & Organizer Recognition...... 6 Sponsor Recognition...... 7 Sponsor Descriptions...... 8 Plenary Sessions...... 10 Biographies...... 12 Post-Conference Field Trip...... 15 Agenda-at-a-Glance...... 16 Detailed Agenda...... 17 Poster Display Information...... 26 Poster Directory...... 27 PDHs for Engineers and Continuing Education...... 30 Additional Information...... 31 Abstract Compilation...... 33 Notes...... 192

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April 23-26, 2018 | Coral Springs, Florida, USA Welcome Letter Greetings and Welcome to Coral Springs!

The Biogeochemistry Laboratory, a research unit within the UF/IFAS Soil and Water Sciences Department, is very excited to host the 12th International Symposium on Biogeochemistry of Wetlands, April 23-26, 2018. We welcome your participation in this symposium.

The objective of this international symposium is to provide a framework for scientists to discuss new topics related to biogeochemistry of nutrients and other contaminants in freshwater and coastal wetlands. The focus of the symposium will be on “Protecting the Future of Water." Wetland systems serve as sinks, sources and transformers of nutrients and other chemical contaminants and therefore can have a significant impact on water quality and productivity of . The primary driver of these processes is ecosystem biogeochemistry, and includes the intersection of chemical, physical and biological processes in the soil and water column. These processes operate at various spatial and temporal scales. Our location here in Coral Springs, Florida can’t help but remind us of the fragile of our wetland systems. South Florida’s Everglades, recognized internationally, encompasses nearly 18,000 square miles of the southern tip of the Florida peninsula – from the Kissimmee River basin all the way south to the shores of Florida Bay and the Gulf of Mexico. Historically this vast, free-flowing, shallow river of grass provided clean water and pristine habitats, supported numerous wading and migratory birds, as well as Florida Panthers, manatees and deer. However, the system has been widely impacted by levees, canal-associated drainage projects and nutrient loading, primarily . We hope you will take an opportunity to see this amazing wetland in person while you are here.

While listening to the exciting research being presented, remember that the International Symposium on Biogeochemistry of Wetlands is an opportunity for all of us to meet new colleagues, reunite with old ones, and learn what research is ongoing around the globe. The Conference Planning and Program Committees worked diligently to develop a wide ranging and inclusive lineup of talks and posters focused on wetland biogeochemistry. We hope you enjoy the program of presentations and have an opportunity to teach others about what you do and where you work.

Finally, a few words of appreciation. We would like to first thank our generous Sponsors listed on page 7, whose financial support is critical to making this conference a reality. We are also grateful to those who gave of their time and expertise to organize and moderate sessions, and to share their expertise with us. Further, we would be remiss without thanking all of the many individuals recognized on page 6, who volunteered their personal time and energy to organize and plan this conference. And of course, we would also like to thank those who submitted abstracts, are giving talks and are presenting posters. Were it not for you and the work you do, we would not have had the privilege of organizing such a great symposium.

Have a great week, enjoy yourself, and when you leave, we trust you will take away new information, new connections, and new tools and knowledge you can use to advance wetland science in your respective corners of the globe.

Best regards,

Todd Z. Osborne John R. White Conference Chair Conference Co-Chair Whitney Laboratory for Marine Bioscience & Coastal Sciences Department University of Florida Louisiana State University

5 12th International Symposium on Biogeochemistry of Wetlands Committee & Organizer Recognition

Planning Committee

Dr. Todd Z. Osborne, Conference Chair, Assistant Professor, UF/IFAS Soil and Water Sciences Department, University of Florida, Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA

Dr. John R. White, Conference Co-Chair, Professor, Louisiana State University, Department of Oceanography and Coastal Sciences, Baton Rouge, LA, USA

Dr. K. Ramesh Reddy, Conference Advisor, Graduate Research Professor and Chair, UF/IFAS Soil and Water Sciences Department, Gainesville, FL, USA

Program Committee and Session Organizers

Professionals from the wetlands biogeochemistry science community organized sessions on topics relevant to their restoration programs. This process took several months and numerous volunteer hours. We would like to thank the following individuals for their time, effort and expertise on behalf of the symposium.

Session # Organizers

1, 4 Dr. Patrick Megonigal, Smithsonian Environmental Research Center, Edgewater, MD, USA

2 Dr. David Krabbenhoft, U.S. Geological Survey, Middleton, WI, USA

3 Dr. Ilka Feller, Smithsonian Environmental Research Center, Edgewater, MD, USA

5, 8 Dr. Patrick Inglett, University of Florida, Soil and Water Sciences Department, Gainesville, FL, USA

6 Dr. Lauren Kinsman-Costello, Kent State University, Kent, OH, USA

7 Dr. Sue Newman, South Florida Water Management District (SFWMD), West Palm Beach, FL, USA

9 Prof. Curtis Richardson, Duke University Wetland Center, Durham, NC, USA – and – Dr. Jeff Chanton, Florida State University, Tallahassee, FL, USA

10, 14, 18 Dr. Gail Chmura, McGill University, Montreal, Quebec, Canada

11 Dr. Todd Osborne, University of Florida, Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA

12 Ms. Kimberli Ponzio, St. Johns River Water Management District (SJRWMD), Palatka, FL, USA

16 Dr. John White, Louisiana State University, Baton Rouge, LA, USA

20 Dr. Odi Villapando, South Florida Water Management District (SFWMD), West Palm Beach, FL, USA

23 Dr. Anna Knox, Savannah River National Laboratory, Aiken, SC, USA

24 Dr. Jacob Berkowitz, U.S. Army Corps of Engineers, Vicksburg, MS, USA

6 April 23-26, 2018 | Coral Springs, Florida, USA Sponsor Recognition

Thank you to all of the organizations that supported this premier event. This symposium would not be possible with you.

Silver Sponsors

DB Environmental, Inc. Louisiana Sea Grant Louisiana State University | College of the Coast & Environment OTT Hydromet Picarro, Inc. University of Central Florida | National Center for Integrated Coastal Research University of Florida Office of Research UF/IFAS Research UF/IFAS Soil & Water Sciences Department

Bronze Sponsors

City of Orlando University of Florida, Whitney Laboratory for Marine Bioscience

7 12th International Symposium on Biogeochemistry of Wetlands Sponsor Descriptions

Please join us in thanking all of our sponsors for investing their time, resources and finances in support of the symposium. We could not have done it without you.

Silver Sponsors

DB Environmental, Inc. DB Environmental, Inc., (DBE) is a wetland research firm with substantial expertise in aquatic biogeochemistry, and modeling. For decades, DBE has been a key scientific consultant on some of the world’s largest and most prominent wetlands treatment. DBE’s problem-solving efforts for both government and private clients have included enhancement of vegetation communities, development of long-term soil management practices, and optimization of hydraulic and water quality characteristics.

Louisiana State University (LSU) College of the Coast & Environment www.lsu.edu/cce/ LSU is at the forefront of coastal environmental issues. Its coastal legacy dates back three-quarters of a century. Consulting and collaborating on coastal research on every continent, the College of the Coast & Environment equips graduates with multi-disciplinary tools to address real-world issues, and leads research to better understand the causes, impacts (unanticipated, forecasted, and actual) and restoration strategies for today’s most pressing coastal and environmental challenges/threats.

Louisiana Sea Grant www.laseagrant.org/ Louisiana Sea Grant, based at Louisiana State University, is part of the National Sea Grant Program, a network made up of 33 programs located in each of the coastal and Great Lakes states and Puerto Rico. Sea Grant Programs work individually and in partnership to address major marine and coastal challenges. LSU was designated the nation’s thirteenth Sea Grant College in 1978. Louisiana Sea Grant had its Program Review in October 2015, conducted by the National Sea Grant Office/NOAA, and LSG was rated as “exceeds expectations by a substantial margin in some areas/aspects.”

OTT Hydromet www.ott.com Designing answers by providing sustainable solutions that go beyond the expectations of Hydromet professionals to support them in making informed decisions for caring for the world’s water resources and forecasting surface weather. OTT Hydromet Group offers the combined strength and expertise of leaders in the fields of General Meteorology, Weather Critical Operations, as well as Water quality and Quantity.

Picarro, Inc. www.picarro.com Picarro is the world's leading provider of stable isotope and gas concentration measurement systems for many scientific applications. The ultra-precise and easy-to-use instruments are deployed across the globe offering unmatched performance and enabling scientists around the world to measure GHGs, trace gases and stable isotopes found in the air, water, and land.

8 April 23-26, 2018 | Coral Springs, Florida, USA

University of Central Florida (UCF) National Center for Integrated Coastal Research www.ucf.edu/faculty/cluster/sustainable-coastal-systems/ The mission of the National Center for Integrated Coastal Research is to assess natural and human-related impacts to the health, restoration, and of coastal systems; conduct long-term, integrated, multi-disciplinary research with strategic external partners; communicate findings in an effective and efficient way; communicate information to the general public; and train the next generation of scientists to enter the workforce as science emissaries, with interdisciplinary skills.

UF/IFAS Research www.research.ifas.ufl.edu/ The research mission of UF/IFAS, conducted under the auspices of the Florida Agricultural Experiment Station (FAES), is to discover new scientific knowledge, encourage innovative study and create applications based on sound science that address challenges facing agriculture, natural resources, and interrelated human systems in Florida, our country, and the world.

UF/IFAS Soil & Water Sciences Department www.soils.ifas.ufl.edu/ Soil and water are vital resources in urban, agricultural, and natural ecosystems. The Soil and Water Sciences Department (SWSD) provides highly visible leadership in teaching, research, and extension/outreach programs as related to improving the productivity of agriculture with environmentally sound management practices, improving water quality, and protection and conservation of natural resources. Our department is one of the few in the nation that offers comprehensive research and educational programs (molecular to landscape level) involving terrestrial, wetlands and aquatic ecosystems of the landscape. In addition to traditional on-campus educational programs, we use innovative e-technologies to offer educational programs to place-bound students. Our graduates and postdoctoral fellows are well placed at universities, state and federal agencies, and private industry.

UF Office of Research www.research.ufl.edu/ Research at UF plays a significant role in advancing our fundamental understanding of the universe, generating creative breakthroughs that lead to technologies with positive benefit, creating opportunities for economic growth in the state, and changing the trajectory of young people through education. The Office of Research is committed to facilitating the research and scholarship successes of our faculty and students by creating effective collisions between researchers and funding opportunities, marketing our research capabilities to collaborators and funding agencies, and forging institutional relationships with external stakeholders. Bronze Sponsors

City of Orlando www.cityoforlando.net/wetlands/ The City of Orlando’s Water Reclamation Division owns and operates the Orlando Easterly Wetlands, which is one of the first large scale constructed wetland treatment systems designed to polish reclaimed water. The City of Orlando and the Orlando Easterly Wetlands are proud to sponsor the 12th International Symposium on Biogeochemistry of Wetlands.

University of Florida Whitney Laboratory for Marine Bioscience www.whitney.ufl.edu/ The University of Florida Whitney Laboratory for Marine Bioscience, located in St. Augustine, FL, uses marine models to study fundamental problems in and then applies that knowledge to issues of human health, natural resources and the environment. We do this by performing research in 10 faculty led labs, training future scientists, promoting and providing lifelong learning and advancing conservation through our Sea Turtle Hospital and Oyster Restoration Initiative, and other environmental programs.

9 12th International Symposium on Biogeochemistry of Wetlands Plenary Sessions

Tuesday, April 24, 2018 8:30am – 10:00am | Royal Poinciana

Welcome Address Jack M. Payne, Ph.D. Senior Vice President for Agriculture and Natural Resources University of Florida Institute of Food and Agricultural Sciences (IFAS) Gainesville, FL, USA

Plenary Presentation Coastal Environmental Settings as a Model to Explain Global Controls of Storage in Mangroves Robert R. Twilley, Ph.D. Executive Director, Louisiana Sea Grant College Program Professor, Oceanography and Coastal Sciences, Louisiana State University Baton Rouge, LA, USA Presentation Description

Mangrove forests are found in dynamic coastal landforms controlled by physical forcings (e.g. river, tides and waves) and geomorphic evolutionary processes (e.g. rate and source of sediment input, and rate of relative sea-level change). Combined, these processes create a range of clastic coastal environments spanning from prograding to transgressive embayed shorelines. Dr. Twilley’s presentation will discuss how the environmental drivers - wave power, tidal and fluvial processes - along with regional climate, produce conspicuous coastal environmental settings, including deltas, estuaries, lagoons and karstic landforms. Built on the ecogeomorphogy framework, the coastal environmental setting approach recognizes hydrology, , and climate as main components to macrosystem-scale variation in coastal wetland’s ecological processes. Hence, each coastal setting has a conspicuous environmental signature that controls both the and rate of sediment supply to the coastline, nutrient load and limitation (e.g. -to-phosphorus stoichiometric ratio), organic matter diagenesis, and, ultimately, C storage in vegetation (above- and belowground biomass) and soil pools. This presentation will further discuss how considering nitrogen (N) and phosphorus (P) density in soils may represent conditions limiting macrosystem-scale C stocks, C-N-P stoichiometric interactions may enhance or weaken the carbon-climate feedback. Thus, reconciling site-specific mechanisms regulating C:N:P stoichiometry with macrosystem-scale processes in mangrove ecosystems is key to advance conceptual models that can improve our capacity to predict global C stocks and rates of sequestration in these and other coastal wetlands.

10 April 23-26, 2018 | Coral Springs, Florida, USA

Wednesday, April 25, 2018 8:30am – 10:00am | Royal Poinciana

Impacts of Hurricane Irma on the Everglades and South Florida Ecosystem Nick G. Aumen, Ph.D. Regional Science Advisor - South Florida Center for Collaborative Research, US Geological Survey Davie, FL, USA

Plenary Presentation Carbon Remineralization and Burial in the Coastal Margin: Linkages in the Thomas S. Bianchi, Ph.D. Jon and Beverly Thompson Endowed Chair of Geological Sciences Department of Geological Sciences, University of Florida Gainesville, FL, USA Presentation Description

Continental margin systems collectively receive and store vast amounts of organic carbon (OC) derived from primary productivity both on land and in the , thereby playing a central role in the global . The land-ocean interface is however extremely heterogeneous in terms of terrigenous inputs, marine primary productivity, sediment transport processes and, depositional conditions such as bottom water levels. Continental margins are also highly dynamic, with processes occurring over a broad range of spatial and temporal scales. The rates of organic carbon burial and oxidation are consequently variable over both space and time, hindering our ability to derive a global picture of OC cycling at the land-ocean interface. In this presentation, Dr. Bianchi will review the processes controlling the fate of organic matter in continental margin sediments with a special emphasis on “hot spots” and “hot moments” of OC burial and oxidation. In addition, he will present a compilation of compositional data from a set of illustrative settings, including fjords, small mountainous river margins, large deltaic systems and upwelling areas.

11 12th International Symposium on Biogeochemistry of Wetlands Biographies Symposium Chairs

Todd Z. Osborne, Ph.D., Conference Chair Assistant Professor of Coastal Biogeochemistry University of Florida, Whitney Laboratory for Marine Bioscience St. Augustine, FL, USA

My interest in was catalyzed by continual exposure to the wonders of the natural world at a young age which, in turn, led to degrees in biology, environmental engineering and biogeochemistry. As a faculty member at the Whitney Laboratory for Marine Bioscience in St. Augustine, FL, I seek to meld my scientific interests and training with a lifelong passion for the environment. Arguably, there is no better way to do this than to investigate the intricate ecological processes that define coastal ecosystems.

Estuaries lie at the nexus of freshwaters (rivers, streams, wetlands) and the ocean. These incredibly complex and ecologically important ecosystems are experiencing effects of global climate change in ways that we are only just beginning to understand. Defining the biogeochemical processes and the resulting cascade of ecological effects that climate change brings to our coastal ecosystems is at the center of my research focus. , mangrove range expansion and coastal are issues of great importance locally and globally and are thus the priority areas of interests for my research program. In addition to estuarine research, I have been very active in the Everglades Restoration arena in the last 15 years and maintain active research in that incredible ecosystem. A co-appointment in the Soil and Water Sciences Department at the University of Florida affords me the opportunity to continue research in many diverse aspects of the Florida Everglades, from agriculture and nutrient management to landscape biogeochemical cycles.

John R. White, Ph.D., Conference Co-Chair John and Catherine Day Professor of Oceanography & Coastal Sciences Wetland & Aquatic Biogeochemistry Laboratory, Louisiana State University Baton Rouge, LA, USA

My research interests in the environment were forged at an early age as I wandered along the beaches of New England collecting rocks and sand dollars, which eventually led to degrees in , Geological Oceanography and Soil and Water Science. At LSU for the past 14 years, I am engaged in research in a coastal setting that intersects the largest river discharge deltaic system in North America with the largest coastal marsh system in the lower 48 states. A high relative sea level rise rate, due to coupled global eustatic sea level and regional subsidence, has led to a dramatic loss of coastal wetland area. This dynamic setting, from freshwater to brackish to marine, allows us to investigate natural and anthropogenic stressors on biogeochemical functions including carbon sequestration, water quality function, greenhouse gas release rates, primary production and phosphorus cycling in the context of wetland creation, wetland restoration and coastal hypoxia.

The results of this research are used to inform policy makers as part of the Coastal Restoration Program in Louisiana on effective measures as the state seeks to overcome almost of century of coastal land loss. I currently serve as the Associate Director of the Coastal Studies Institute at LSU, on the Governor’s Steering Committee on the State Master Plan for Louisiana and on the US Environmental Protection Agency Board of Scientific Counselors for Safe and Sustainable Water Resources.

12 April 23-26, 2018 | Coral Springs, Florida, USA

Plenary Speakers

Nicholas G. Aumen, Ph.D. Regional Science Advisor - South Florida Center for Collaborative Research, U.S. Geological Survey Davie, FL, USA

Dr. Nick Aumen is Regional Science Advisor for the US Geological Survey (Southeast Region), overseeing the Greater Everglades Priority Ecosystem Sciences program. This program, involving USGS scientists nationwide, provides high quality science in support of Everglades restoration. Nick was an aquatic ecologist for 15 years with Everglades National Park, leading an interagency team of scientists tracking restoration progress. Prior to his National Park Service position, Nick was the Research Director at the South Florida Water Management District, directing a team of 120-plus scientists conducting research in support of ecosystem restoration. Nick received his B.S. and M.S. in biology at the University of West Florida, and his Ph.D. in microbial at Oregon State University. He was a faculty member in the Biology Department at the University of Mississippi, and was a tenured Associate Professor of Biology when he returned to Florida. Nick presently is an affiliate faculty member at Florida Atlantic University (Department of Geosciences), and at the University of Florida (Soil and Water Sciences Department). He also served five years on the national Board of Directors of the Sierra Club, a 120-yr-old environmental organization with more than 750,000 members, and served two terms as its Vice-President and one as Treasurer.

Thomas S. Bianchi, Ph.D. Jon and Beverly Thompson Endowed Chair of Geological Sciences Department of Geological Sciences, University of Florida Gainesville, FL, USA

Dr. Tom Bianchi is a full professor and holder of the Jon and Beverly Thompson Endowed Chair in Geological Sciences at the University of Florida (UF), Gainesville, Florida. Before joining UF I held full professor positions at Tulane University and Texas A&M University. My general areas of expertise are organic , chemical oceanography, and global carbon cycling in aquatic ecosystems. I have published over 210 articles in refereed journals and am sole and/or co-author of 7 books, with another book on Chemical Oceanography of the Gulf of Mexico - due out in 2018. I am currently Editor-in-Chief of the journal Marine , and have served as an Associate Editor for numerous other journals. I am the recipient of two Fulbright Research Awards, became a Fellow of the American Association for the Advancement of Science (AAAS) in 2012, and in 2017 was named Geochemical Fellow of the Geochemical Society (GS) and The European Association of Geochemistry (EAG), and Fellow of the Association for the Sciences of and Oceanography (ASLO).

Many of the central issues in research concerning global climate change involve understanding the exchange and transport of organic and inorganic pools of carbon – in the context of the global carbon budget. If we are to successfully balance and model global carbon fluxes, it is important to understand the dynamics of carbon cycling in the most productive environments. In general, the most productive environments are located in land-margin ecosystems such as watershed soils, freshwater, and marine coastal systems. During the past few years my research has centered on organic carbon cycling from source-to-sink with work focused on the transport of soils in watersheds of large river systems to coastal environments. Dr. Bianchi has used state-of-the-art techniques to determine the role of terrestrial versus aquatic carbon sources in the overall carbon cycles of these ecosystems.

13 12th International Symposium on Biogeochemistry of Wetlands

Jack M. Payne, Ph.D. Senior Vice President for Agriculture and Natural Resources University of Florida Institute of Food and Agricultural Sciences (IFAS) Gainesville, FL, USA

Jack Payne is the Senior Vice President for Agriculture and Natural Resources at the University of Florida and the Administrative Head for the Institute of Food and Agricultural Sciences. Prior to his current position he served as a Vice President at Iowa State University, and, previous to Iowa State, he was a Vice President and Dean at Utah State University. Jack also has experience at two other land-grant institutions: Pennsylvania State University, where he served on the faculty of the School of Forest Resources, and, later, at Texas A&M University, where he served as a faculty member in the Fisheries and Wildlife Department.

After leaving Texas A&M University, Payne had a long career with Ducks Unlimited (DU), as their National Director of Conservation. While at Ducks Unlimited, some of his successes included the development of DU’s private lands program with agriculture, the development of a national conservation easement program and the expansion of their Mexican program to Central and .

Payne received his M.S. in Aquatic Ecology and his Ph.D. in Wildlife Ecology from Utah State University and is a graduate of the Institute for Educational Management at Harvard University. He is a tenured professor in the Department of Wildlife Ecology and Conservation at the University of Florida.

Robert R. Twilley, Ph.D. Executive Director, Louisiana Sea Grant College Program Professor, Oceanography and Coastal Sciences, Louisiana State University President, Coastal and Estuarine Research Federation (CERF) Baton Rouge, LA, USA

Dr. Twilley is Executive Director of Louisiana Sea Grant College Program and professor in the Department of Oceanography and Coastal Science at LSU. For the past two years, Robert has served as President-of Coastal and Estuarine Research Federation, an international organization of scientists and managers that focus on coastal issues. In 2017, Dr. Twilley received the National Wetlands Award in Science Research, presented at the Botanic Gardens in Washington DC by the Environmental Law Institute. At LSU, Dr. Twilley has been a Distinguished Professor in Oceanography and Coastal Science, served as Associate Vice Chancellor of Research, Director of the Wetland Biogeochemistry Institute, and founder of the LSU Coastal Sustainability Studio. During his tenure at University of Louisiana at Lafayette, Robert served as Vice President of Research, was a Distinguished Professor in Biology Department, and developed the UL Lafayette Center for Ecology and .

Robert Twilley has developed a career balancing productive contributions in university research, leadership in university program development, and science communication associated with public policy concerning the value of ecosystem restoration. Robert also served the state of Louisiana in several capacities as science advisor to coastal restoration planning projects including Louisiana Coastal Area (LCA) plan, 2007 and 2012 Louisiana Coastal Master Plans, and regional planning following Hurricanes Katrina and Rita known at Louisiana Speaks. He initiated the development of the Coastal Emergency Risk Assessment (CERA) software to visualize storm surge forecasts of coastal flooding associated with tropical cyclones. Robert Twilley has been instrumental in developing strong communication and collaboration with several state, federal and non-governmental organizations (NGO) in supporting the role of science in large scale ecosystem restoration projects. He has testified in several US House and Senate subcommittee hearings and worked with staff from several congressional and state subcommittees, along with briefings to EPA, CEQ, OSTP, DOI, NOAA and OMB to develop policy for coastal restoration programs.

14 April 23-26, 2018 | Coral Springs, Florida, USA Post-Conference Field Trip Loxahatchee Impoundment Landscape Assessment (LILA) 10216 Lee Road Boynton Beach, FL 33437

Thursday, April 26, 2018 | 12:00 PM – 5:30 PM | Cost to Participate: $50.00 [Limited to 15 people]

Description The Loxahatchee Impoundment Landscape Assessment (LILA) is located on the grounds of the Arthur R. Marshall Loxahatchee National Wildlife Refuge in Boynton Beach, Florida. LILA is a working, 80-acre model of the Everglades ecosystem. This “living laboratory” gives experts an opportunity to research and apply restoration techniques on a small, controlled scale before taking them into the 1.7 million-acre Everglades ecosystem.

Field Trip Itinerary

Thursday, April 26, 2018

12:00 PM Immediately after closing plenary, go to the bus loading zone at Marriott [Conference Center Entrance]

12:15 PM Board Bus and Depart

1:00 PM Stop at Visitor Center for Overview of Refuge and LILA

1:15 PM Welcome Introduction and Overview to the Refuge

1:30 PM Begin Walking Tour around LILA

2:45 PM Arrive at canoe outpost - sign out canoes and jackets

3:00 PM Canoe into the Refuge

Bus picks group up at canoe outpost 4:15 PM Pit-stop at Visitor Center before departure

4:45 PM Depart for Hotel

5:30 PM Arrive at Coral Springs Marriott

We Provide: Transportation, lunch, refreshments and canoe rental with life jacket.

You Bring: Sunscreen, insect repellent, water bottle, old tennis shoes or water shoes that can get wet. Light clothing that dries quickly is ideal. Weather will be hot – dress accordingly. You may also want to bring a camera and binoculars. LILA tours entail about a 1.5 mile walk and include a tour of two tree islands and a walk around two macrocosms. Comfortable shoes are a must!

15 12th International Symposium on Biogeochemistry of Wetlands Agenda-at-a-Glance

Monday, April 23, 2018 4:00pm-6:00pm Poster Presenters and Sponsors Set Up Displays (Orchid Ballroom)

4:00pm-7:00pm Registration Open (Conference Center Foyer and Palm Room)

6:00pm-8:00pm Welcome Reception (Breeze’s Terrace) Tuesday, April 24, 2018 7:30am-8:30am Morning Refreshments (Orchid Ballroom)

7:30am-5:00pm Registration Open (Conference Center Foyer and Palm Room)

8:30am-10:00am Opening General Session (Royal Poinciana)

12noon Lunch Buffet (Orchid Ballroom and Breeze’s Terrace)

10:30am-5:00pm Three Concurrent Sessions (Royal Poinciana, Ibis and Egret)

5:00pm-7:00pm Poster Session Reception (Orchid Ballroom) Wednesday, April 25, 2018 7:30am-8:30am Morning Refreshments (Orchid Ballroom)

7:30am-5:00pm Registration Open (Conference Center Foyer and Palm Room)

8:30am-10:00am General Session (Royal Poinciana)

12noon Lunch Buffet (Orchid Ballroom and Breeze’s Terrace)

10:30am-5:00pm Four Concurrent Sessions (Royal Poinciana, Ibis, Egret & Sandpiper)

5:00pm-7:00pm Poster Session Reception (Orchid Ballroom) Thursday, April 26, 2018 7:30am-8:30am Morning Refreshments (Orchid Ballroom)

7:30am-12noon Registration Open (Conference Center Foyer and Palm Room)

8:30am-10:00am Three Concurrent Sessions (Royal Poinciana, Ibis & Egret)

10:00am-10:30am Poster Presenters and Sponsors Remove Displays

10:30am-12noon Closing General Session (Royal Poinciana)

12noon Symposium Concludes

Optional Post-Conference Field Trip to Loxahatchee Impoundment Landscape 12:00noon-5:30pm Assessment (LILA). Shuttle departs from Conference Center Entrance.

16 April 23-26, 2018 | Coral Springs, Florida, USA Detailed Agenda

Monday, April 23, 2018

Poster Presenters and Sponsors Set Up Displays 4:00pm-6:00pm [Conference Center - Orchid Ballroom] Symposium Registration Open 4:00pm-7:00pm [Conference Center - Palm Foyer] Welcome Reception on Breeze’s Terrace 6:00pm-8:00pm (Please plan to arrive in time to join us!)

Tuesday, April 24, 2018

Symposium Registration Open 7:30am-5:00pm [Conference Center - Palm Foyer] 7:30am-8:30am Morning Refreshments in Poster & Sponsor Display Area

Opening General Session [Royal Poinciana]

Introductory Remarks 8:30am Dr. Todd Osborne, Assistant Professor, University of Florida/IFAS Soil and Water Sciences Department, Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA -and- Dr. John White, Professor, Louisiana State University, Department of Oceanography and Coastal Sciences, Baton Rouge, LA, USA

Welcome Address 8:45am Dr. Jack Payne, Senior Vice President for Agriculture and Natural Resources, University of Florida/IFAS, Gainesville, FL, USA

Plenary Presentation Coastal Environmental Settings as a Model to Explain Global Controls of Carbon Storage in Mangroves 9:00am Dr. Robert Twilley, Executive Director, Louisiana Sea Grant College Program Professor, Oceanography and Coastal Sciences, Baton Rouge, LA, USA

AM Refreshment Break in Poster & Sponsor Display Area 10:00am-10:30am [Conference Center - Orchid Ballroom]

17 12th International Symposium on Biogeochemistry of Wetlands

Tuesday, April 24, 2018 (continued)

Concurrent Sessions [10:30am - 12:00noon] Session 1 Session 2 Session 3 Royal Poinciana Ibis Egret New and Emerging Tools and Nitrous and Techniques for the Shifts in Foundation Oxide Cycling in Wetlands Study of Biogeochemistry Species and Upland Forests (Part 1) in Wetlands Pat McGonigal David Krabbenhoft Ilka Feller 10:30am Introduction & Overview Introduction & Overview Introduction & Overview Sunitha Pangala Brian Bergamaschi Samantha Chapman Large Methane Emissions Tidal Wetland Warming Facilitates from Amazon Floodplain Biogeochemistry in High Mangrove Encroachment 10:35am Trees Definition: Using High- and Alters Belowground Frequency Measurements Processes to Estimate Biogeochemical Rates Kristofer Covey Collin Eagles-Smith Catherine Lovelock Methane in Upland Forest Flow Cytometry as a Fluctuating Sea Level and Trees Novel, Rapid, Screening Habitat Change in Western 10:50am and Research Tool For Australia Methylmercury Production Activity in Aquatic Ecosystems Mari Pihlatie Sarah Janssen Loraé Simpson -Mediated Methane From Cellular to Global: Effects of Nutrient and Canopy Exchange in a Using Mercury Stable Enrichment on the Carbon 11:05am Boreal Upland Forest Isotopes to Understand Dynamics in the Salt Marsh Mercury Cycling and - Mangrove Ecotone Sources Kazuhiko Terazawa Brett Poulin Anne Ola Vertical Patterns of CH4 Molecular- and Atomic- The Roots of Blue Carbon Emission along Tree Stems Level Approaches to in Mangrove Forests: The 11:20am of Alnus japonica and Characterize Dissolved Effects of Soil Properties on Fraxinus mandshurica Organic Matter: Insights for Stilt Root Development in Mercury Bioavailability in Rhizophora stylosa the Florida Everglades Joost van Haren William Orem Emily Dangremond Large Methane Emissions Methods of Extreme Events and 11:35am from Palm Stems in Analysis in Wetlands and Historical Regime Shifts in Amazonian and Flood Applications to Studies of the Mangrove-Salt Marsh Lands Mercury Biogeochemistry Ecotone 11:50am Q&A Q&A Q&A 12noon-1:30pm Group Luncheon Buffet

18 April 23-26, 2018 | Coral Springs, Florida, USA

Tuesday, April 24, 2018 (continued)

Concurrent Sessions [1:30pm - 3:00pm] Session 4 Session 5 Session 6 Royal Poinciana Ibis Egret Methane and Nitrous Wetland Enzymes in a The Novel Biogeochemistry Oxide Cycling in Wetlands Changing Environment of Ubiquitous Understudied and Upland Forests (Part 2) (Part 1) Urban Wetlands Sunitha Pangala Patrick Inglett Ashley Smyth 1:30pm Introduction & Overview Introduction & Overview Introduction & Overview Pat Megonigal Colin Jackson Alexander Reisinger Methane Emissions from What Controls Microbial Challenges of Connectivity 1:35pm both Wetland and Upland Enzyme Activity in Within Urban Landscapes: Trees across a Flooding Wetlands? Examples From The Gradient Baltimore Ecosystem Study Zhiping Wang Patrick Inglett Lauren Kinsman-Costello Methane Emissions from Warming Rate Drives Urban Stormwater 1:50pm the Stems of Living Trees in Microbial Nutrient Wetlands As Novel Upland Forests Limitation and Enzyme Biogeochemical Systems: Expression Elevated Salt and Sulfate Rodrigo Vargas Hojeong Kang Eban Bean Automated Measurements PH Controls Phenol Oxidase A Little Retro: Valuing 2:05pm of CO2, CH4, and N2O and DOC Leaching From an Undersized Urban Fluxes from Tree Stems and Global Peatland Stormwater Wetland Adjacent Soils Retrofit Paul Brewer Chris Freeman Ariane Peralta High-Frequency Tree Sequestering Carbon in How Do We Manage CH4 Flux Measurements Wetlands Through Enzyme Microbiomes to Promote 2:20pm Reveal Relationships Suppression Urban Wetland Functions? with Tree Physiology and Environmental Properties Christopher Schadt Monica Palta Methanogenic Accidental Urban Wetlands: 2:35pm Dominate Mature Populus Biogeochemical Processes deltoides Heartwood Q&A in Unexpected Places Habitats 2:50pm Q&A Q&A PM Refreshment Break in Poster & Sponsor Display Area 3:00pm-3:30pm [Conference Center - Orchid Ballroom]

19 12th International Symposium on Biogeochemistry of Wetlands

Tuesday, April 24, 2018 (continued)

Concurrent Sessions [3:30pm - 5:00pm] Session 7 Session 8 Session 9 Royal Poinciana Ibis Egret Agents and Causes of Methane and Nitrous Wetland Enzymes in a Peat Stability and GHG Oxide Cycling in Wetlands Changing Environment Ratios Across Moisture, and Upland Forests (Part 3) (Part 2) Temperature & Latitude Gradients Curtis Richardson and Jeff Dongqi Wang Kanika Inglett 3:30pm Chanton Introduction & Overview Introduction & Overview Introduction & Overview Ashley Smyth Stephanie T. Castle Jeff Chanton Time Series Soil Oxygen Linking Microbial A Global Latitudinal Data Help Identify Hot Extracellular Enzyme Gradient in Peatland 3:35pm Spots and Hot Moments of Activities with Rates of Organic Matter Chemistry Greenhouse Gas Emissions Plant Litter Decay From Wetlands Dongqi Wang Nic Vermeulen Curtis Richardson Effects of Temperature Use of Bacterial Chemical Controls on Increasing On the Nitrous Transformation in Carbon Sequestration and 3:50pm Oxide Emission from Processing Non- GHG Flux Along A Boreal To Intertidal Area along the Biodegradeable Plastics Tropical Gradient East China Coast Jeroen De Klein Zuhair AlQulaiti Maite Martinez-Eixarch Greenhouse Gas Emissions Hydrological Variation and Greenhouse Gas Emissions from Wetlands with Enzymic Decomposition in and Carbon Sequestration 4:05pm Different Vegetation Type Wetlands in Mediterranean Rice Fields and Wetlands: The Ebro Delta Case Iikka Haikarainen Kathleen Pietro Hongjun Wang Methane Fluxes of Trees Microbial Enzyme Activity Does An ‘Iron-Gate’ and Forest Floor under in a Stormwater Treatment Regulate Drought Effects On 4:20pm Two Different Water Level Ares in Response to Inflow Peat Decomposition? Condition in Forestry Flow Conditions Drained Peatland in Southern Finland Kanika Inglett Neal Flanagan Stoichiometric Controls of Thermal Alteration of Microbial Enzyme Activities Peat By Low-Severity Fire 4:35pm on Nutrient Cycling In Reduces Net Carbon Loss To Q&A Wetlands Microbial Respiration

4:50pm Q&A Q&A Poster Session Reception I 5:00pm-7:00pm (Poster presenters at ODD NUMBERED BOARDS to be available for questions and discussion from 6pm - 7pm.)

20 April 23-26, 2018 | Coral Springs, Florida, USA

Wednesday, April 25, 2018

Symposium Registration Open 7:30am-5:00pm [Conference Center - Palm Foyer] Morning Refreshments in Poster & Sponsor Display Area 7:30am-8:30am [Conference Center - Orchid Ballroom]

General Session [Royal Poinciana]

8:30am Introductory Remarks Dr. K. Ramesh Reddy, Graduate Research Professor and Chair, UF/IFAS Soil and Water Sciences Department, Gainesville, FL, USA

Impacts of Hurricane Irma on the Everglades and South Florida Ecosystem 8:40am Dr. Nick Aumen, Regional Science Advisor - South Florida, Center for Collaborative Research, U.S. Geological Survey, Davie, FL, USA

Plenary Presentation Carbon Remineralization and Burial in the Coastal Margin: Linkages in the Anthropocene 9:00am Dr. Thomas S. Bianchi, Jon and Beverly Thompson Endowed Chair of Geological Sciences, Department of Geological Sciences, University of Florida, Gainesville, FL, USA

10:00am-10:30am AM Refreshment Break in Poster & Sponsor Display Area

21 12th International Symposium on Biogeochemistry of Wetlands

Wednesday, April 25, 2018 (continued)

Concurrent Sessions [10:30am - 12noon] Session 10 Session 11 Session 12 Session 13 Royal Poinciana Ibis Egret Sandpiper Blue Carbon: Improving Biogeochemical Responses Wetland Management Data Applied to IPCC to Saltwater Transgression Effects on Carbon Coastal Processes Emission Factors and Events in the Marine Sequestration and (Part 1) Carbon Markets (Part 1) Environment Greenhouse Gasses Gail Chmura Todd Osborne Kimberli Ponzio Loraé Simpson 10:30am Introduction & Overview Introduction & Overview Introduction & Overview Introduction & Overview Junsung Noh Jayantha Obeysekera Janet Ho John Nyman Capacity Change in Organic An Overview of Global and Characterizing How Nutrients Interact With Carbon Storage in Intertidal Regional Sea Level Rise Biogeochemical Shifts in Stresses, such as Flooding Flat During Drainage After Projections: Means and Two Shrub Encroached and Salinity, to Affect 10:35am Reclamation: Case Study in Extremes Marshes under Different Wetland Plant Growth and Saemangeum, Korea Historical Disturbance Leaf Tissue Stoichiometry Regimes in the St. Johns River, FL Gail Chmura Benjamin Wilson Dave Sumner Shelby Servais Blue Carbon Losses with Drivers and Mechanisms Role of Hydroperiod and Effects of Increased Salinity Salt Marsh Drainage of Peat Collapse in Coastal Fire on Carbon Dynamics of on Microbial Processing of 10:50am Wetlands a Subtropical Peat Marsh Carbon and Nutrients in Brackish and Freshwater Wetland Soils Andre Rovai Lisa Chambers Brian Benscoter Katie Bowes Global Controls of Carbon Short-Term Response of Shifting Fire Regimes and Sediment Phosphorus Storage in Mangrove Soils Freshwater Wetland Soils to the Future of Peatland Speciation and Distribution 11:05am Saltwater Intrusion Carbon Storage in Coastal LA Sediments: Implications for Hypoxia and Food Web Dynamics Guangcheng Chen Scott Neubauer Angelique Bochnak Joshua Papacek Considerations in Blue Persistent Saltwater Subsidy Stress Gradient in Have We Reached a New Carbon Accounting with Intrusion Alters Ecosystem a Peat-based Floodplain Normal?: Nutrient Cycling Mangrove Restoration - Carbon Cycling In Tidal Marsh and Bloom Dynamics in 11:20am Case Studies from South Freshwater Marshes: the Northern Indian River Fujian, China Comparison of Results from Lagoon In Situ Manipulations in Virginia and South Carolina Havalend Steinmuller Todd Osborne Barclay Shoemaker Shaofeng Pei Fate of Translating the Effects of Carbon Cycling and Nutrient Dynamics and Following Sea Level Rise- Sea-Level Rise in Urban Potential Soil Accumulation Their Interaction with 11:35am Induced Coastal Wetland Systems to the Coastal within Coastal Forested Phytoplankton Growth in Submergence: a Microcosm Ecosystem Interface Wetlands the Aquatic Areas of Coastal Experiment Wetland in Liaohe Delta, China 11:50am Q&A Q&A Q&A Q&A 12noon-1:30pm Group Luncheon Buffet

22 April 23-26, 2018 | Coral Springs, Florida, USA

Wednesday, April 25, 2018 (continued)

Concurrent Sessions [1:30pm - 3:00pm] Session 14 Session 15 Session 16 Session 17 Royal Poinciana Ibis Egret Sandpiper Blue Carbon: Improving Nitrogen and Phosphorus Hydrological Restoration: Data Applied to IPCC Coastal Processes Dynamics in Aquatic Reconnection of Wetland Emission Factors and (Part 2) Systems Ecosystems Carbon Markets (Part 2) Gail Chmura Kevin Grace John White Lisa Chambers 1:30pm Introduction & Overview Introduction & Overview Introduction & Overview Introduction & Overview Derrick Vaughn Hilary Flower Greg Noe Tianna Picquet Blue Carbon Sequestration Shifting Ground: Landscape- Nutrient and Sediment Novel Interactions May Within a Northeastern Scale Modeling Of Soil Inputs Change Soil Structure Affect Range Expansions: Is 1:35pm Florida Intertidal Wetland Biogeochemistry under and Biogeochemistry in Heavy Ungulate Browsing - Response to Climate Climate Change in the Floodplain Ecosystems: A Restraining Mangrove Change and Holocene Florida Everglades Cross-Study Synthesis Advance On The South Climate Variability Texas Coast? Elise Morrison Paul Julian Sara McMillan Derek Detweiler The Role of Priming Effects One Of These Things Effects of Floodplain Phytosterols as Tracers of on the Conversion of Blue Is Not Like The Other. Restoration on Nitrogen and Terrestrial and Wetland

Carbon to CO2 in the Coastal Evaluation of Wetland Phosphorus Dynamics in Carbon to Ten Thousand 1:50pm Zone Nutrient Stoichiometry Agricultural Watersheds Islands, Florida, USA: and Homeostasis in a Implications for Trophic Subtropical Treatment Resource Usage in the Wetland Eastern Oyster, Crassostrea Virginica Amy Borde Sara Phelps Natalie Peyronnin Hayley Craig Measuring Continuous Reevaluating the Rebuilding Mississippi River Factors Controlling Diversity Greenhouse Gas Fluxes Consequences of Land Use: Delta: Operating a Sediment and Composition of Soil 2:05pm from Pacific Northwest Accelerated Dissolution Diversion to Balance Microbial Communities in Tidal Wetland Sediments of Geologic Ecosystem and Social Needs Mangroves Following Salt-Water Deposition in Humic Lakes Intrusion Thomas Mozdzer Alina Spera Nia Hurst Michael Wessel Nutrient Enrichment Alters Effect of Hydrologic Reducing Nitrogen Developing a Nutrient Blue Carbon Pools and Restoration on Coastal Removal Uncertainty for Management Strategy for Processes Wetland Soil Properties Operation of Mississippi Southwest Florida Tidal 2:20pm River Sediment Diversions: Creeks by Linking Source Nitrate Reduction Rates In Water Concentrations, Turbulent Flow Conditions Instream Processes, and Estuarine Dynamics Siyuan Ye William Mitsch John White Jessica Vaccare Carbon Sequestration and Sustainably Solving Legacy Evaluating Nitrate Reduction The Impacts of Corexit Its Controlling Factors in Phosphorus and Nitrogen in in a Hydrologically Restored EC9500A on Wetland the Temperate Wetland Landscapes with Wetlands Bottomland Hardwood Microbial Activity and 2:35pm Communities Along the and Wetlaculture Forest: Is Reconnection Community Structure in Bohai Sea, China Improving Water Quality Barataria Bay, LA., USA Function?

2:50pm Q&A Q&A Q&A Q&A 3:00pm-3:30pm PM Refreshment Break in Poster & Sponsor Display Area

23 12th International Symposium on Biogeochemistry of Wetlands

Wednesday, April 25, 2018 (continued)

Concurrent Sessions [3:30pm - 5:00pm] Session 18 Session 19 Session 20 Session 21 Royal Poinciana Ibis Egret Sandpiper Blue Carbon: Improving Biogeochemical Studies Data Applied to IPCC Influence of Large- Toward Improving Emission Factors and scale Restoration on Performance of the Treatment Wetlands Carbon Markets Biogeochemical Processes Everglades Stormwater Part 3 Treatment Areas Gail Chmura Fred Sklar Odi Villapando Mark Sees 3:30pm Introduction & Overview Introduction & Overview Introduction & Overview Introduction & Overview Joseph Smoak Sue Newman Len Scinto Carles Ibanez Coupled Soil Carbon Restoration of Settling and Entrainment Dynamics of Metals, Measurements and Remote Biogeochemical Properties of Particulates in Nutrients, Sediments and Sensing to Quantify Above Characteristics through the STAs Carbon in Mediterranean 3:35pm and Belowground Carbon Active Management of Constructed Wetlands Stocks in Mangrove Forest the Nutrient-Enriched Receiving Agricultural of the Ten Thousand Islands Everglades (CHIP) Runoff Region of Southwest Florida, USA Joshua Breithaupt Erik Tate-Boldt Patrick Inglett Li Zhang Are Carbon Burial Rates Biogeochemical Drivers Hydrologic Flow Effects on Hydrological Regime in the Coastal Everglades of Aquatic Ecosystem Microbial Stoichiometry Impacts on Macrophyte 3:50pm Higher Now Than They Metabolism under an and Enzyme Activity in the Communities of Urban Were a Century Ago? Altered Flow Regime in an Everglades Stormwater Stormwater Treatment Everglades Marsh Treatment Areas Wetlands in Southwest Florida Jeffrey Kelleway Colin Saunders Lauren Griffiths Taylor Nesbit What is the Carbon Flow Impacts on P and OM Nutrient Retention via Seasonal and Hurricane Sequestration Potential Cycling across Everglades Vegetative Uptake and Irma Effects on the of Australia’s Coastal Ridge and Slough: Lessons Sedimentation in Created Hydrologic Regime of 4:05pm Floodplain Forests? from Landscape Budgets Wetlands in Subtropical A Constructed Urban in the DECOMP Physical Florida Stormwater Treatment Model and Shark Slough, Wetland Complex in ENP Southwestern Florida Bong-Oh Kwon Christa Zweig Mike Jerauld R. Thomas James Carbon Storage Capacity Active Management Phosphorus Flux in the Effects of Hydrology, Time of Estuarine Tidal Flat and Influences on Everglades Stormwater and Inflow Concentration 4:20pm Salt Marsh in the West and Biogeochemistry in a Treatment Areas on Phosphorus Discharge South Coasts of Korea Nutrient-Poor Wetland from a Periphyton-Based Stormwater Treatment Area Stephen Crooks Mark Cook Odi Villapando Ronald Corstanje Inclusion of Coastal Faunal Contributions to P Biogeochemical Response Big Data Applied to the Wetlands in United States Cycling and their Influence of Selected STA Flow-ways Stormwater Treatment 4:35pm Inventory of Greenhouse on Restoration of the to Different Flow Scenarios Areas in The Everglades, Gas Emissions and Sinks Everglades Mapping Out System Resilience 4:50pm Q&A Q&A Q&A Q&A Poster Session Reception II 5:00pm-7:00pm (Poster presenters at EVEN NUMBERED BOARDS to be available for questions and discussion from 6pm - 7pm.)

24 April 23-26, 2018 | Coral Springs, Florida, USA

Thursday, April 26, 2018

Symposium Registration Open 7:30am-12noon [Conference Center - Palm Foyer] 7:30am-8:30am Morning Refreshments in Poster & Sponsor Display Area Concurrent Sessions [8:30am - 10:00am] Session 22 Session 23 Session 24 Royal Poinciana Ibis Egret Wetland Soil Biogeochemical Processes Contaminant Removal in Biogeochemistry in Created in South Florida Ecosystems Wetlands and Restored Environments Mike Jerauld Anna Sophia Knox Jacob Berkowitz 8:30am Introduction & Overview Introduction & Overview Introduction & Overview Kevin Grace Matt Huddleston Christine VanZomeren Nutrient Exchange Savannah River Site’s A-01 Biogeochemical Response Dynamics Following Constructed Wetland of Coastal Wetland Soil 8:35am Sediment Resuspension in System: A Model for to Thin Layer Sediment South Florida Wetlands Sustainable Aquatic Risk Application Mitigation Jennifer Cooper Anna Sophia Knox Jacob Berkowitz Influence of Metal Mobility and Rapid Formation of Precipitation and Aquatic Retention in Constructed Potential Acid Sulfide 8:50am Vegetation on Phosphorus Wetland Sediment Soils Following Wetland Removal in Canal Water Restoration – A Cautionary from the Everglades Tale Agricultural Area Barry Rosen Michael H. Paller Kim Oldenborg The Role of Cyanobacteria Using DGT to Measure Impacts of Sediment in Nucleating the Bioavailable Metals in Dredging on Phosphorus 9:05am Precipitation of Calcium A Constructed Wetland Dynamics of a Restored Carbonate in the Treatment System Riparian Wetland Everglades: Vaterite and Aragonite Hanh Nguyen Xiaoyu Xu Hongjun Chen Microbial Composition of Do Constructed Wetlands Dissolved Oxygen Sag 9:20am Everglades Stormwater Remove Metals or Increase Events in the Phase I Area Treatment Areas is Linked to Metal Bioavailability? of The Kissimmee River Restoration Project Richard Baker Sanjana Banerjee Masanori Fujimoto Biogeochemistry of Trace Turning a Liability into Spatial Variability in Elements in Card Sound, an Asset: Can We Use Microbial-Mediated Florida, Inventory and the Invasive Apple Snail Biogeochemical Processes 9:35am Annual Turnover Circa 1973 Pomacea Maculata in Everglades Stormwater In Biomonitoring Of Treatment Areas Metal Contamination In Freshwater Marshes? 9:50am Q&A Q&A Q&A AM Refreshment Break 10:00am-10:30am Poster Presenters and Sponsors Remove Displays 10:30am-12noon Closing General Session 12noon Symposium Concludes Optional Post-Conference Field Trip – Loxahatchee Impoundment Landscape Assessment (LILA) 12noon-5:30pm Participants to gather in lobby next to registration to prepare for departure.

25 12th International Symposium on Biogeochemistry of Wetlands Poster Display Information

Poster presentations play a key role in the exchange of information at BioGeo 2018. Considerable time will be dedicated for viewing them. Posters will be on display throughout the conference in the Poster & Sponsor Display Room (Orchid Ballroom). This is the primary gathering spot where morning, mid-day and afternoon refreshments are served. The agenda also features an evening Poster Session Reception on Tuesday & Wednesday. These networking functions maximize opportunities for discussion amongst poster presenters, attendees and sponsors.

• Formal poster sessions are scheduled Tuesday (5pm–7pm; session 1) and Wednesday (5pm–7pm; session 2). • Poster presenters are to stand at their poster during the assigned poster session. Please do not stand at your poster during the poster session in which you are not assigned.

Monday, April 23 | 4:00PM – 6:00PM Posters may be set-up upon arrival at the hotel any time during these hours, but POSTER SET UP no later than 10:30am Tuesday, April 24. All posters are on display throughout the conference. Posters are to be removed Thursday during the mid-morning break.

Tuesday, April 24 | 5:00PM – 7:00PM POSTER SESSION 1 (Presenters at ODD NUMBERED BOARDS are to stand at their posters 6pm-7pm.)

Wednesday, April 25 | 5:00PM – 7:00PM POSTER SESSION 2 (Presenters at EVEN NUMBERED BOARDS are to stand at their posters 6pm-7pm.)

POSTER REMOVAL Thursday, April 26 | 10:00AM – 10:30AM

* Organizers are not responsible for lost posters not removed from the boards, and discarded by the board vendor.

26 April 23-26, 2018 | Coral Springs, Florida, USA Poster Directory

(Presenters listed in alphabetical order by last name)

Poster First Name Last Name Organization City ST Country Abstract Title No. Soil Nutrient Enrichment Post University of United 5 Kaylee August Gainesville FL Hydrologic Management: A Temporal Florida States Analysis Of Taylor Slough East Carolina United Greenhouse Gas Potential of a 6 Regina Bledsoe Greenville NC University States Constructed Stormwater Wetland Louisiana Understandings Mechanisms For Baton United 32 Ashley Booth State LA Marsh Sustainability in the Face of Sea Rouge States University Level Rise South Florida Restoration Benefits Observed From Water West Palm United 35 Bahram Charkhian FL the Biscayne Bay Coastal Wetlands Management Beach States Project District South Florida Long-Term Treatment Performance Water West Palm United 7 Michael Chimney FL of Constructed Wetlands Built for Management Beach States Everglades Restoration District PBL Neth. Environmental Towards a Global Model for Wetlands 30 Jeroen de Klein The Hague Netherlands Assessment Ecosystem Services Agency Comparison of Heat Flux Reduction in Florida United a Wetland Modular Rooftop Garden 8 Brittany Dolan Gulf Coast Fort Myers FL States System and a Xeric Rooftop Garden University System in Southwest Florida Phosphorous Analyses Including Florida United P-31 Nuclear Magnetic Resonance 19 Bobby Duersch Atlantic Boca Raton FL States in the C51 Basin and University Northern Everglades Investigation of Ecosystem Services Provided by Seasonally Emergent College of United 25 Rebecca Flock Nampa ID Wetland Between an Agricultural Western Idaho States Drain and Irrigation Catchment in Southwest Idaho Loxahatchee United Enterococci in Wrack Sediments and 27 Rachel Harris Jupiter FL River District States Surface Water What is the Metal Content of Gravel Missouri State United 20 Jordan Heiman Springfield MO Bar Vegetation in a Contaminated University States River? Hurricane Irma: Biogeochemical University of United 33 Nia Hurst Orlando FL Responses in a Mangrove Encroached Central Florida States Florida Salt Marsh

27 12th International Symposium on Biogeochemistry of Wetlands

Poster First Name Last Name Organization City ST Country Abstract Title No. Temporal Patterns in Enzyme Activity University of United and Bacterial Community Structure 23 Colin Jackson University MS Mississippi States of the Phyllosphere of the Wetland Macrophyte Typha Latifolia Effects of Floodplain Restoration Purdue West United 31 Alex Johnson IN in Agricultural Watersheds on University Lafayette States Phosphorus Dynamics River Runs Through It: Evaluation University of United of Groundwater and Surface Water 24 Paul Julian Fort Pierce FL Florida States Connectivity and its Implication on Riparian Biogeochemistry and Ecology National Park United Effects of Hurricane Irma on Coastal 36 Christopher Kavanagh Key Largo FL Service States Everglades Waters in Florida Bay Carbon Budget of an Eastern North East Carolina United 16 Sydni Law Greenville NC Carolina Coastal Freshwater Wetland University States Following Phosphorus Addition Assessing Significant of Different Florida United Environmental Drivers Impact Coastal 34 Jing Liu Atlantic Boca Raton FL States Wetlands Elevation Change in University Louisiana Institute of Subtropical Dynamics of Soil Nitrogen Agriculture, Accumulation Following Vegetation 14 Xin Liu Changsha Hunan China Chinese Restoration in a Typical Karst Academy of Catchment Sciences Impacts of Restoration on University of United Biogeochemistry of Intertidal Oyster 3 Bryan Locher Orlando FL Central Florida States Reefs in the Indian River Lagoon, Florida Constructed Wetlands for Saltwater Florida Aquaculture Wastewater Treatment United 9 Cristina Lopardo Gulf Coast Fort Myers FL Using a Floating Treatment Wetland States University Bioreactor System and Wetlands Modified with Biodegradable Plastics Florida Microscale Hydrogen Peroxide United 29 Luka Ndungu Gulf Coast Fort Myers FL Measurements of Cyanobacterial States University Blooms in Southwest Florida Lakes School Of Mangrove Forest Biomass Estimates, Geosciences, United Community Structure and 15 Chukwuebuka Nwobi Edinburgh University Of Kingdom Classification in the Nigerian Niger Edinburgh Delta Florida Variation in Carbon Storage and United 13 Danielle Ogurcak International Miami FL Nutrients in Mangrove Across States University Puerto Rico Ecological Consequences of Invasive University of United Plant Species Introduction to Volcanic 2 Eliska Rejmankova California, Davis CA States Lakes in Central America: An Example Davis of Lake Atitlan, Guatemala

28 April 23-26, 2018 | Coral Springs, Florida, USA

Poster First Name Last Name Organization City ST Country Abstract Title No. Changes in Carbon and Nitrogen in University of United a Drained Subtropical Wetland. The 12 Andres Rodriguez Gainesville FL Florida States Case of the Everglades Agricultural Area Effects Of Hurricane Irma on Dissolved University of United 4 Tracey Schafer Gainesville FL Organic Carbon Fluxes Along a Salinity Florida States Gradient City of United The Orlando Easterly Wetlands – 10 Mark Sees Christmas FL Orlando States 30 Years of Constructed Success How Can the Invasive Submersed Macrophytes Stay So Highly Productive Biology Centre Ceske Czech 1 Dagmara Sirova in a Nitrogen Limited Lake? Molecular CAS Budejovice Republic Analysis of Associated Diazotrophs May Provide the Answer Bioaccumulation and Effects of University of Cadmium in Three Phytoplankton Tabassum- United 21 Sabrina Louisiana at Lafayette LA Species: Akashiwo Sanguinea, Tackett States Lafayette Heterosigma Akashiwo, & Coscinodiscus Sp Evaluating Different Salts as Proxies University of United 11 Mohsen Tootoonchi Davie FL For Brackish Water in Sea Level Rise Florida States and Climate Change Research Complete Genome Sequencing Florida United Revealed the , Adaptation 28 Hidetoshi Urakawa Gulf Coast Fort Myers FL States and Cosmopolitan Distribution of University Nitrosospira Lacus Species Influence on Methane University of United 26 Joost van Haren Tucson AZ Emissions from Tree Stems from Arizona States Amazonian Peat and Flood Lands Anton de Kom University of Mercury Deposition in Rivers of 22 Arioene Vreedzaam Suriname/ Wanica Suriname Suriname Tulane University Institute of Methane Emissions from the Stems of 17 Zhiping Wang Beijing China , CAS Living Trees in Upland Forests Florida United Extreme Events Alter C Dynamics 18 Junbin Zhao International Miami FL States Across the Florida Everglades University

29 12th International Symposium on Biogeochemistry of Wetlands PDHs for Engineers and

Continuing Education

CEUs & PDHs for Attendees with Professional Certifications If you are a licensed engineer or maintain a professional license issued by a society, an association, an occupational licensing board or a department of professional regulation within your state, you may be eligible to earn Continuing Education Units (CEUs) for your participation in this conference. You will need to contact the appropriate authority who manages your professional certification to verify your organization or individual state's licensing requirements, and to confirm what documentation is required. While we are not approved as an official CEU provider, your state may recognize this event as a qualified program, and you may be eligible to earn CEUs for your participation.

Record of Attendance Log at Registration Desk A Record of Attendance Log will be maintained at the Conference Registration Desk where you can sign in and sign out each day and create a record verifying your daily attendance. Within 30 days upon conclusion of the conference, we will email you a "Certificate of Attendance" indicating the actual number of contact hours you accrued based on your participation hours recorded in the attendance log. It is your responsibility to compile all necessary paperwork and provide it to the appropriate licensing board or professional organization with whom you are certified, and to confirm this program content is acceptable based on their individual standards.

IMPORTANT NOTE: In general, one Continuing Education Unit (CEU) is defined as 10 hours of instruction. One hour is calculated as 50 minutes of face-to-face instruction. If you have any questions regarding CEU requirements as they pertain to your professional certification or license, please directly contact the appropriate licensing board within your state. The University of Florida, Office of Conferences & Institutes and its employees are not authorized to act on your behalf or to provide consult regarding CEUs.

30 April 23-26, 2018 | Coral Springs, Florida, USA Additional Information

Free Internet Access Complimentary wireless Internet access will be available to BioGeo 2018 attendees in the meeting space, hotel lobby, and public & lounge areas. The passcode is different for access in your guest room. Be sure to obtain the most current complimentary access code from the front desk when you check in. To access Internet in the conference center, follow these instructions on your device:

1. Connect to the network “Marriott_CONF” 2. You will be directed to the splash page (Attending Meeting) where you will enter: Conference Code: BioGeo2018 3. Click on "I agree to the terms of use" 4. Click on log in

Name Badge Your name badge will serve as your pass while attending the symposium, so please be sure to wear it while attending all functions. If arriving Monday, you should pick up your name badge and materials that evening to avoid the Tuesday morning rush. Conference Registration is open 4:00pm-7:00pm Monday, April 23 in the Conference Center building. It reopens Tuesday morning at 7:30am. Please be sure to register all guests and pay the applicable registration fees. Guests must also wear their name badges for entry into functions. The guest fee allows guests 18 years of age and older to attend the Welcome Social on Monday evening and the Poster Session Networking Receptions on Tuesday and Wednesday. The guest fee does not include meeting attendance.

Lost & Found When an article is lost, please first check the conference registration area. If the lost article(s) cannot be found, check with the hotel’s front desk staff.

Remember to Join Us on Social Media! Follow us on Twitter @biogeo2018 and like us on Facebook @biogeo2018 to see our posts. Use the hashtag #biogeo2018 to stay connected with your fellow conference attendees. www.conference.ifas.ufl.edu/biogeo2018

31

April 23-26, 2018 | Coral Springs, Florida, USA

Abstract Compilation

The following abstracts are listed alphabetically by presenting author’s last name. Presenting author names appear in bold.

33

April 23-26, 2018 | Coral Springs, Florida, USA

HYDROLOGICAL VARIATION AND ENZYMIC DECOMPOSITION IN WETLANDS Zuhair AlQulaiti1, Christian Dunn1, Tim Jones1 Sandrine Hugron2, Line Rochefort2, Chris Freeman1 1Bangor University, Wales, UK 2PERG, Laval University, Quebec, Canada

Despite covering only 3% of the Earth’s land surface, boreal and subarctic peatlands store about 15-30% of the world’s soil carbon as peat. Historically, peatlands have contributed to global cooling on a millennium timescale. Whether peatlands will continue their function as net C sinks remains uncertain, and depends on the impact of environmental factors, not least of which is hydrological conditions. This issue is particularly important in Canada, where more than 12% of the land area is covered by peatlands, which means around 56% of organic carbon stored in that soil, and thus Canada was selected as the focus of this study. Hydrological conditions can radically influence decomposition in peat, and such conditions are anticipated to become more extreme under changing climate. Moreover, the impairment of enzyme activities (through the enzymic latch) in wetlands has an important role in increasing carbon sequestration by reducing the decomposition of organic matter. The main objective of this study is to determine the effect of the fluctuation water table level on the decomposition under the following conditions:

1) Stable water table between 0 and -5 cm (Control) 2) Stable water table between -10 and -15 cm 3) Stable water table between -20 and -25 cm 4) Fluctuating water table between +5 and -35 cm over 10 days cycle period, 5) Fluctuating water table between +5 and -35 cm over 30 days cycle period.

These treatments created substantial changes in the enzyme activities associated with decomposition, with the greatest impacts seen in the systems experiencing the greatest hydrological variability. Our findings suggest that climate change towards a climate system with more hydrological variability threatens to destabilize our peatland carbon stores. The interdependency of respiration rates, transport, and air filled porosity, changes in water content near saturation will most likely have a strong impact on rates of respiration in the unsaturated zone of many peat soils.

BIO: Zuhair AlQulaiti, is a PhD student with 3 years of experience of wetland enzyme research. He is developing experience in studying carbon cycling, the influence of decomposition on greenhouse gas emissions.

Contact Information: Zuhair AlQulaiti, Wetland Research Group, School of Biological Sciences, Bangor University, Bangor, Wales, LL57 2UW, UK

35 12th International Symposium on Biogeochemistry of Wetlands

SOIL NUTRIENT ENRICHMENT POST HYDROLOGIC MANAGEMENT: A TEMPORAL ANALYSIS OF TAYLOR SLOUGH Kaylee August1,2, Paul Julian1, and Todd Osborne1,2 1oil and ater ciences epartment, niversity of lorida, ainesville, , hitney aboratory for arine ioscience, niversity of lorida, t. ugustine, ,

aylor lough, a dominant drainage feature in northeastern Everglades ational ar, is a landscape with predominantly marl and organic soils. he slough receives flow from rainfall and water control structures directly north. Water management practices varied in intensity and scope from the 1960’s to the early 2000’s, resulting in variable phosphorus loading to the slough which has contributed to vegetation shifts from sawgrass to cattail. ew best management practices s lead to highly reduced concentrations in the water column, however, recent studies have identified variability of topography, hydrology, and ecology to be significant factors driving enrichment. his study aims to compare two sampling periods to further assess enrichment and cattail epansion by evaluating soil properties. oil nutrient properties total carbon , total nitrogen , total phosphorus , inorganic , loss on ignition , and bul density were collected on two separate occasions and 1 in the wet season. he study observed a consistent range of values for all soil nutrient properties in and 1, while almost all ecept bul density had lower overall mean concentrations for 1. he later sampling ehibited greater inorganic 1 mg g1 spread in the soil as compared to 5 mg g1. ul density displayed increased spread in the 1 sampling period as compared to , with ranges from .11. g cm and ..1 g cm respectively. he rainfall and stage data show lower averages in 1 as well. he lower stage and rainfall averages in 1 lead to greater variability of inorganic , but decreased the overall average of in the soil. hough stagerainfall was lower, inorganic phosphorus fractions were greater in spread in 1 and increased. n conclusion, lower stage and rainfall lead to increased oidation of organic materials and greater variability of inorganic fractions within the system. he loss of the historic organic soils could lead to the everincreasing enrichment of the Everglades, potentially epanding cattail presence in aylor lough.

aylee ugust is a graduate student at the niversity of lorida oil and ater ciences epartment and the hitney aboratory for arine iosciences under r. odd sborne. Her research involves phosphorus enrichment and cattail epansion in aylor lough of the Everglades ational ar.

ontact nformation aylee ugust, niversity of lorida, 1 carty r., ainesville,

36 April 23-26, 2018 | Coral Springs, Florida, USA

BIOGEOCHEMISTRY OF TRACE ELEMENTS IN CARD SOUND, FLORIDA INVENTORY AND ANNUAL TURNOVER CIRCA 1973 Joseph L. Gilio Presented by: Richard Baker oseph . ilio, independent researcher, alm ity, ,

n inventory of trace transition elements anadium , opper u, admium d, ead as developed for the ard ound ecosystem circa 19. his or determined metal concentrations of ater and organisms collected and analyed in 19 this study ith simultaneous studies of geology, sediment and plant and animal populations and productivies using the same ard ound grid y respective eperts during 190. his sutropical calcareous ased, moderately flushed emayment as then on the fringes of rapid development from the iami metrople and neary nuclear poer plant. rganisms and ater samples this study and sediment analysis y ellenarg,19 ere analyed y reservoir atomic asorption spectrophotometry. he procedure eliminated matri interference. ccuracy compared to rchid eaves as e 9, u 92, n96, d100 and 9 n 19, no iological standard for eisted. ignificant differences ithin given species trace metal concentrations eisted ased on location ithin ard ound. or mass model development and iological flues, such aerial differences ere comined to produce a threeo model of organisms, ater, and total sediments of ard ound, lorida.

his model incorporated the entire sediment volume masses of mean depth of 6 cm. uch a small total sediment volume of calcareous sediment was expected to magnify the influence of the ecosystem’s biota in the interactions between sediment, water and iota and their . n agreement ith other studies, the total ecosystem mass of , e, u, n, d, and ere highest in sediment hereas ater mass for and e ere greater than the total iota standing stoc. iota mass alance for u n, d and ere greater than ater column ut less than sediment. halassia testudinum turtle grass had trace element standing stoc highest for all elements, folloed closely y sponges, ith large rooted calcareous algae approimately an order of magnitude loer. orals ere lacing. hytoplanton, epiphytes, and inverterates constituted minor components of the iological trace element inventory. race elements sins in ard ound ca. 19 ere sediment and iota.

maor forcing function for trace transition movement from sediment and ater as net primary production of turtle grass and its epiphytes, respectively, through 90 g dry t.m2yr or 2 of the iological dry t. matter production of the sound horhaug, 19. nnual net primary and secondary production incorporated 2.1 more e and .9 more than all other iota. et photosynthesis production across all primary produces –seagrasses, epiphytes, calcareous algae incorporated g yr e 1. 10 n . 10 1. 10 u . 10 2 . l 0 1 and d 1.0 l0 1. urtle grass lade multiplication and groth accounted for maor movement of e, , and d from sediments into this species and its seasonal lade death and decay return to the sediment. aonomic iota groups concentrated trace elements in decreasing order of e, n, , u, , and d. urtle grass sediment uptae yielded residence times of 1 days for n and 20 days for d. ll others e, , u and sediment residence times ranged from centuries to millennia. n nutrient limitation is proaly avoided especially if turtle grass dynamic” pumping” from water also exists, then implications for releases from nuclear poer into such caronate rich thin sediment areas indicate high moility of n 6 from sediment into the iological food chain. idal ater dynamics transported 1.2 e and copper into and out of ard ound than iological incorporation. idal echange dynamics indicate oceanic echanges directly ith a small part of ard ound, so that iological cycling of trace elements appears more important than tidal flushing. idal echange netted outflos of n and d and a net inflo of e, u, and . ard ound ater concentrations influenced y tidal echange mass alance dynamics compared oceanic flushing ith iological turnover daysgday –e 09, n 29., u 11.0, 0.096, d 600.02.

his research originally represented dissertation reuirements for the octor of hilosophy degree in arine cience at the osenstiel chool of arine and tmospheric cience, niversity of iami, lorida and as performed eteen 191 . ssistance of rs. ouglas egar and oert ellenarg are gratefully acnoledged. his paper originally as prepared for the ea rant symposium on iscayne ay, iami, lorida pril 196. eer revie delay prevented its inclusion in the original ound symposium.

r. ilio, an active retired W meritus, taught at –ensen each, founded, ran and sold Wetlands anagement nc. e designs and researches ater uality improvement for laes and etlands. ,00 ac. ousing proects achieved for . supplemental to at .oegilio.com

ontact nformation oseph ilio, 0 W t. ucie hores r., alm ity, 990,

37 12th International Symposium on Biogeochemistry of Wetlands

TURNING A LIABILITY INTO AN ASSET: CAN WE USE THE INVASIVE APPLE SNAIL POMACEA MACULATA IN BIOMONITORING OF METAL CONTAMINATION IN FRESHWATER MARSHES? Sanjana Banerjee1, Paul L. Klerks1, Harry J. Whitlow1,2 and Naresh T. Deoli1,2 niersity of ouisiana at afayette, afayette, , ouisiana ccelerator enter, afayette, ,

he apple snail Pomacea maculata is an inasie species that has recently been introduced and is now present in seeral ouisiana marsh areas he introduction and range expansion of this species are bound to hae a ariety of ecological conseuences ne potential effect is that the presence of this species will modify the biogeochemical cycling of metals oweer, the introduction may also hae a faorable aspect by proiding us with a species that may be well suited for biomonitoring for enironmental contaminants such as heay metals

We are currently conducting research that will provide insights into both aspects of the snail’s introduction. In the laboratory, snails were maintained in water at a range of leels of a metal such as copper u and the metal leels are being uantified in their gills, lung, idney, gut, digestie and reproductie glands esults of these analyses will proide an indication whether the snails are efficient metal bioaccumulators and what tissues are best suited for biomonitoring n order to determine the relationship between metal bioaccumulation in the apple snail and metal leels and distribution in the enironment, we are comparing metal leels in snail tissues, plants, sediment and water collected from two sites that differ in physicochemical parameters

eels of the metal lead b are also being uantified in shells and operculum, with a noel method employing article nduced ay mission which will proide information on patterns of accumulation of lead he research is ongoing results will be presented at the meeting

anana aneree is a h student woring in nironmental oxicology under the guidance of r aul lers riginally from ndia, she graduated with and degrees in oology he is interested in the ecotoxicological conseuences of the introduction of the inasie snail Pomacea maculata and their use as biomonitors

ontact nformation anana aneree, h student a fellow, epartment of iology and nstitute for oastal ater esearch, niersity of ouisiana at afayette, afayette, ,

38 April 23-26, 2018 | Coral Springs, Florida, USA

A LITTLE RETRO: VALUING AN UNDERSIZED URBAN STORMWATER WETLAND RETROFIT Eban Z. Bean1, Ariane Peralta2, Gina Bledsoe2, Charles Humphrey3, and Michael A. O’Driscoll4 gricultural and iological ngineering epartent niversity of lorida ainesville iological ciences epartent ast arolina niversity reenville ealth ducation and rootion epartent ast arolina niversity reenville eological ciences epartent ast arolina niversity reenville

egacy storwater systes priarily designed for efficient drainage have led to channel erosion and sedientation as well as nutrients and pathogen eports that have degraded and ipaired urban streas. urrent regulations for ost unicipalities reuire storwater control easures to address the ipacts of increased ipervious cover. owever developed landscapes can constrain retrofit options as eisting infrastructure and available space prevent certain options while liiting the sie of other options. ndersiing storwater control easures is a possible solution for retrofitting urban watersheds if the scalability of functions can be understood better. reens ill un is a coastal plain strea ipaired by ecess storwater runoff fro the urban core of Greenville, North Carolina. At nearly 50% impervious, East Carolina University’s (ECU) main capus is a significant contributor of storwater volues and nutrient loads. In a dry detention basin was regraded to construct the ar rinson torwater Wetland on ECU’s campus. Due to topographic and infrastructure liitations the wetland was only of the typical full design sie for the contributing watershed. low onitoring and water uality sapling of inflows and discharges were perfored between ugust and ugust . Water uality treatent lagged behind conventional credits however flood itigation and other ecosyste services provide typically unuantified value as well. he low residence tie liited water uality treatent. owever other functions flood reduction habitat education etc. have provided ecosyste services and value that can coonly be overlooed.

I r. ean is an assistant professor and etension specialist of urban water resources engineering at the niversity of lorida. uch of his wor focuses on urban storwater anageent. rior to his current position he spent four years at ast arolina niversity as an assistant professor in the ngineering epartent.

ontact Inforation ban . ean gricultural and iological ngineering epartent niversity of lorida owry d uilding o ainesville

39 12th International Symposium on Biogeochemistry of Wetlands

SHIFTING FIRE REGIMES AND THE FUTURE OF PEATLAND CARBON STORAGE Brian W Benscoter lorida Atlantic University, Davie, , UA

Disturance is o critical ecological importance rom populations to ecosystems. Natural systems have an inherent capacity to respond to natural disturances, oten adapting their core attriutes to include disturance in their ecology. ut hat happens hen the disturance itsel ecomes distured Where do the ounds o this capacity lie and hat are the conseuences o eceeding these thresholds n etlands, the interplay o ire and drought eert consideraly inluence on their ecology and caron cycling, particularly peatlands o oreal and tropical iomes. heir thic, caronrich organic soils have adaptive protection mechanisms that decrease soil comustion ris during ildire, even hile in some cases promoting intense surace or canopy ire ehavior. oever, the dense organic soils can ecome vulnerale uels during severe drought, leading to scenarios o more severe ire eects and potentially compromised postire ecosystem resilience. lant invasions, particularly y shrus, urther compromise ecosystem resilience y altering ire ehavior and ecosystem recovery. Caron storage in peatlands is the result o a delicate alance eteen storage and loss ith a ine margin sensitive to etremes. hereore, identiying tipping points in regime change or disturance and its underlying drivers is critical or protecting the resilience o these valuale ecosystems.

Dr. enscoter is Associate roessor o Wetland lant Ecology at lorida Atlantic University and iceChair o the outh Atlantic Chapter o the ociety o Wetland cientists. e has over 5 years o eperience researching etland ire ecology and caron cycling rom suarctic to suoreal iomes.

Contact normation rian W enscoter, lorida Atlantic University, Department o iological ciences, 00 College Ave, Davie,

40 April 23-26, 2018 | Coral Springs, Florida, USA

TIDAL WETLAND BIOGEOCHEMISTRY IN HIGH DEFINITION: USING HIGH-FREQUENCY MEASUREMENTS TO ESTIMATE BIOGEOCHEMICAL RATES Brian A. Bergamaschi r D. Doi r .. rs University U.. Geological urvey, acramento, CA, UA

idal etlands are hydrodynamically comple, varying over temporal scales rom minutes to decades and spatial scales rom centimeters to ilometers. ur understanding o undamental iogeochemical processes in these environments is oten iased y sampling strategies that alias the intrinsic signal y sampling at spatial and temporal intervals ar too coarse to capture true variaility, to accurately uantiy lu or to adeuately relate process rates to spatial and temporal scale. We have een using measurement techniues aimed at improving our understanding o iogeochemical processes, oth to provide insight into mechanisms driving changes as ell as to improve society’s ability to manage and improve our aquatic resources. oever, many challenges remain or improving our understanding o iogeochemical processes in these comple environments.

ecent developments in ield instrumentation have improved our aility to resolve chemical, isotopic and compositional dierences directly in the environment, maing measurements rapidly enough to avoid aliasing the signals o physical and iological processes. easurements using such instrumentation undamentally alter our understanding o iogeochemical processes, and call into uestion the accuracy o monthly, eely, and even daily sampling to assess lu, as ell as the aility o laoratory mesocosms to characterie environmental process rates adeuately. We ill present three eamples o using such instrumentation to determine iogeochemical rates.

We ill sho ho e used insitu optical instrumentation in comination ith acoustic measurements to uantiy constituent lues (g, eg, DC) as ell as eamine changes in sources and processes over daily to seasonal scales in a tidal marsh. We ill also sho ho e used data rom paired insitu, high reuency (5 minute) sensors to evaluate rates o change in nitrate concentration, and then to estimate ater column nitriication rates. inally, e ill sho ho e used the relationship eteen spatial variation o ater residence time and concentration to assess environmental rates, using transects o hydrogen and oygen isotopes in ater (, ) to estimate residence time. hese isotopes are uniue tracers or evaporation and thus may e used in conunction ith independently determined evaporation rates to evaluate ater residence times. he relationship eteen residence time and change in concentration provides a direct measurement o rate.

Although these and other methods represent provide improved means o understanding and uantiying iogochemical rates and processes, sustantial challenges remain. or eample, methods or estalishing the spatial scale o interactions, or longterm assessment and or comparing and etrapolating measured values across spatial scales are needed. We ill discuss opportunities or improvements in eisting methods and directions in the incorporation o high reuency measurements into etland iogeochemical studies.

Dr. ergamaschi is a iogeochemist ith the UG, and adunct aculty ith CU in acramento. e graduated rom University o Washington, here he studied the cycling o natural organic material, eteen siing and climing trips. is interests mainly lie in developing ne methods to uantiy iogeochemical rates and in messing around on oats.

Contact normation rian ergamaschi, UG Caliornia Water cience Center, Caliornia tate University lacer all, 000 treet, acramento, CA 5

41 12th International Symposium on Biogeochemistry of Wetlands

RAPID FORMATION OF POTENTIAL ACID SULFIDE SOILS FOLLOWING WETLAND RESTORATION – A CAUTIONARY TALE Jacob F. Berkowitz risi or rmy orps o ngineers ngineer esearc and evelopment enter icsburg

any coastal etlands contain iron sulide e ic may generate substantial acidity soil p during drougt or disturbance due to oidation and ormation o suluric acid e ormation o iron sulides occurs olloing microbially driven reduction o e and under anaerobic conditions it subsequent cemical compleation o e and lobally increasing numbers o salt marses so signs o degradation due to ragmentation lac o sediment inputs and storm damage ic may be eacerbated by sea level rise and increasing storm requencyintensity s a result resource managers see to restore marses via introduction o sediment to increase mars elevation and oset degradation oever te rapid ormation monts o e materials as been documented in several marses olloing restoration activities tat introduced sediment onto te mars surace leading to concerns regarding restoration outcomes and potential soil acidiication o investigate tese reports a laboratory microcosm study monitored te ormation o e olloing simulated restoration activities esults indicate tat e layers developed itin as e as days encompassing up to o te soil proile itin monts actors inluencing e ormation included ydropattern soil oidation reduction potential and concentration driven diusion gradients o e and ermanently inundated conditions resulted in te igest e content observed compared to drained and simulated tidal eperimental treatments e study igligts te need to consider biogeocemical actors resulting in potential e ormation and soil acidiication during restoration planning implementation and monitoring

r eroit is a researc soil and team leader or etlands researc at te rmy orps ngineer esearc and evelopment enter is or supports etland biogeocemistry assessment and restoration across te nited tates resulting in te publication o ournal articles boo capters and tecnical reports

ontact normation acob eroit ngineer esearc and evelopment enter alls erry d icsburg

42 April 23-26, 2018 | Coral Springs, Florida, USA

Thomas S. Bianchi epto eological ciniv o lorida ainesville lorida

e maority o organic carbon in te global ocean is buried in te coastal margin n particular river delta and nondeltaic sel regions bury an estimated g year− and g year− respectively it only ca g year− buried in te open ocean ile tere as long standing general agreement tat continental selves represent te largest sin o bot terrestrial terr and marine mari in te global ocean our understanding o te spatial and temporal compleity o tis region continues to evolve or eample ords are now more recognized as “hotspots”o carbon burial it recent estimates suggesting ord surace area normalied burial rates are at least ive times greater tan oter marine systems and one undred times greater tan te entire ocean average ere ill compare and contrast some o te ey molecular biomarers tat ave been used to date to trac across dierent depositional environments eg large river deltas and ords and eplore o margintype residence time o transport reservoir dams redo priming eects and molecular stability impact te utility o using dierent biomarers in coastal cycling inally ill ocus on important critical ones itin te aquatic continuum rom landtosea and eamine o more attention is needed better understand cycling in tese ne dynamic interaces in te ntropocene

r ianci is currently a ull proessor and older o te on and everly ompson ndoed air in eological ciences at te niversity o lorida ainesville lorida eore oining eld ull proessor positions at ulane niversity and eas niversity y general areas o epertise are organic geocemistry cemical oceanograpy and global carbon cycling in aquatic ecosystems ave publised over articles in reereed ournals and am sole andor coautor o boos it anoter boo on icl Ocor o l o ico due out in am currently ditorinie o te ournal ri isr and ave served as an ssociate ditor or numerous oter ournals am te recipient o to ulbrigt esearc ards became a llo o Aric Associio or Ac o cic AAA in and in as named ocicl llo o ocicl oci ro Associio o ocisr A and llo o Associio or cics o iolo Ocor AO.

ontact normation omas ianci on and everly ompson ndoed air o eological ciences ept o eological ciences o niversity o lorida ainesville ebsite ttpbiancilabordpresscom ditorinie arine emistry ournalselseviercommarinecemistry

43 12th International Symposium on Biogeochemistry of Wetlands

Regina B. Bledsoe1 . Ari . rl ast aroina niersit reenie niersit o orida ainesie

mperios sraces in ran areas increase stormwater rno eading to nisance ooding onstrcted stormwater wetands s are designed to captre stormwater rno and mitigate ooding in ran areas s can aso proide the additiona eneit o remoing ntrients and potants ie ecosstem serice oweer a negatie conseence o ntrient ccing can occr when enironmenta conditions spport aternatie microia nctions sch as greenhose gas prodction ie ecosstem disserice ew stdies hae addressed this tradeo etween ecosstem nctions in constrcted wetands he oectie o this std is to characterize seasona rates and denitriication potentia in permanent inndated compared to shaow areas within an ran e hpothesized that permanent inndated areas wi hae higher rates than shaow and areas t that shaow areas wi hae simiar denitriication potentias to deep poos r std site is a ha that is adjacent to a 2.3 ha parking lot on East Carolina University’s campus in Greenville, NC. The is diided into ie main zones srace area shaow and shaow water deep poos orea and otet poo e measred es or caron dioide methane and nitros oide rom the inet to the otet in permanent satrated poos and shaow areas throghot the wetand e coected water month or ntrient anasis eg ammonim nitrate phosphate and soi seasona or moistre p and inorganic concentrations e aso determined denitriication potentias o sois sing aased denitriication enzme assa acetene oc method reiminar rests indicate permanent inndated poos hae the highest prodction o s whie shaow and areas prodced the owest rates o prodction haow and sois and permanent satrated sediments were simiar in potentia denitriication rates his indicates that when shaow and is satrated then anaeroic denitriication can proceed to spport compete nitrate remoa eep poos are aorae in s to redce sediment oads and spport denitriication processes howeer these deep poos are maor sorces o methane hs increasing shaow and area within s ma redce tradeos in ecosstem nctions increasing compete nitrate remoa ia denitriication t imiting methane prodction

egina edsoe is a h stdent in the nterdiscipinar rogram o ioogica ciences at ast aroina niersit he is a ationa cience ondation radate esearch eow and a orth aroina ea rant and ater esorce esearch nstitte eow rrent proects inoe nderstanding how microia commnities participate in wetand ntrient ccing

ontact normation egina edsoe epartment o ioog owe cience ompe ast aroina niersit reenie

44 April 23-26, 2018 | Coral Springs, Florida, USA

Angelique M.K. Bochnak1, r illr irli oio Environmental Consulting Technology, nc., Gainesville, lorida, U hitney aoratory o arine ioscience, oil and ater cience epartment, University o lorida, Gainesville, lorida, U ater esources ivision, ureau o Environmental cience, t ohns iver ater anagement istrict, alatka, lorida, U

lood control projects are oten aced ith conlicting management ojectives. etland restoration ased on environmental hydrologic criteria can e at odds ith stage elevations and reuencies resulting rom lood control strategies in the atershed. ncreased hydroperiods have een shon to decrease cover o emergent species and create a gradient that alters vegetative community structure hile decreased hydroperiods result in upland species encroachment and peat loss y susidence. rior to lood control levee construction in , ort rum arsh Conservation rea C as ordered y an incomplete agricultural levee that alloed unregulated drainage o the C to the surrounding loay. ter levee construction, inundation reuencies in several vegetative communities ere increased y up to rom estimated historical averages. These altered hydroperiods ere anticipated to result in heraceous marsh vegetative community dieo ith a shit to an open slough community, as ell as increased soil accretion rates. To determine the implications o lood control management activities in a peatased loodplain marsh, e monitored the vegetative community structure, soil characteristics, and elevation in the C eore and ater levee construction. esults rom these data indicate that the pertured hydrologic regime in C elicited a vegetation response that maimied eloground productivity resulting in a rise in surace elevation o approimately . centimeters per year. This rate is approimately greater than accumulation rates measured eore management action in C. dditionally, total acreage o vegetative communities oserved in postmanagement surveys as ithin o the acreage observed before levee construction. Considering Odum’s perturbation theory on stressgradients, the altered hydroperiods ith the C did not result in a stress to vegetative communities rather, it provided a susidy that resulted in increased ater storage ithout a loss in unction. Thereore, alloing a system suicient time to respond to an input is an important consideration hen measuring restoration success. s such, e suggest that postproject monitoring is vital to successully understanding ecosystem response. s natural resource management strategies adapt to changing climate conditions, longterm monitoring ill e necessary to evaluate the success o management actions and provide data or incorporation into uture approaches.

Contact normation ngeliue .. ochnak, h.., Environmental Consulting Technology, nc., Gainesville,

45 12th International Symposium on Biogeochemistry of Wetlands

Ashley R. Booth1 . i . Ar ouisiana tate niversity chool of eneable atural esources aton ouge .. eological urvey ouisiana Cooperative ish and ildlife esearch nit chool of eneable atural esources aton ouge

Coastal etland loss is prevalent in the Chenier lain of eas and ouisiana a region influenced by altered hydrology subsidence and saltater intrusion. hese etland losses are proected to become more dramatic ith increasing sea level rise associated ith climate change. istorically marshes ere able to eep pace ith sea level rise by gaining elevation through negative feedbac systems that promoted accretion during periods of inundation. o altered hydrology and reduced sedimentation are common in Chenier lain marshes and may be affecting rates of marsh accretion. anagement practices designed to improve ildlife habitat such as ater level manipulation prescribed burning and herbicide usage also affect processes governing marsh elevation though the mechanism of ho this occurs is only partially understood. ltimately any changes to the balance beteen elevation gains and losses ithin these systems ill influence sustainability in relation to sea level rise.

anaged marsh impoundments are particularly vulnerable as ater level dradons designed to increase annual plant production have led to significant elevation losses at multiple sites. hese losses are initially due to increased organic decomposition as the marsh surface is eposed to air. s dradons continue to occur and elevation decreases drainage becomes more difficult and vegetation becomes stressed. tressed plants decrease their beloground productivity and accretion and inadeuate accretion combined ith increasing decomposition rates contribute to eventual marsh loss. n impoundments that can no longer drain plant communities often initially transition to floodtolerant perennial emergents that gro in monodominant stands and produce little food for aterfol. espite their apparent lac of ildlife value there is some interest in managing impounded marshes for perennial emergent vegetation in the hope of increasing soil elevation and longterm marsh sustainability.

o that end our study ill eamine the effects of perennial plant community type ris srlis cirs cliorics and spp. and ater level on changes in processes that govern marsh surface elevation. e ill also evaluate the interplay of these processes on the feedbac systems that influence elevation and ultimately marsh sustainability. t .. urphree ildlife anagement rea in ort rthur eas in a permanently flooded impoundment rodsurface elevation tables ill be used to assess changes in surface elevation. eldspar marer horions root and rhiome decomposition bags and ingroth cores ill be used to measure accretion nutrient dynamics and decomposition rates and beloground productivity respectively. n a nearby impoundment that is periodically dran don soil carbon flu ill be measured in each of the three perennial plant community types. y eamining the relationships beteen the processes that drive marsh sustainability and evaluating the influence of plant community and ater level on marsh surface elevation e ill gain a better understanding of ho marshes behave under permanently inundated conditions. ltimately e hope to inform alternative management strategies that ill increase soil elevation gains in coastal marshes in the Chenier lain and globally thereby bolstering these dynamic ecosystems against increasing sea level rise.

O shley . ooth is a h tudent studying coastal etland ecology at ouisiana tate niversity. he has a bacground in veterinary medicine and completed a Master’s thesis on endocrine disruption in blue crabs in 2016. Her research interests include coastal land loss ethnobotany and science communication.

Contact nformation shley . ooth ouisiana tate niversity chool of eneable atural esources aton ouge

46 April 23-26, 2018 | Coral Springs, Florida, USA

i . Dirr Amy B. Borde lri . lli il Marine ciences aborator aciic orthest ational aborator euim

oastal and luial etland ecosstems near the landard limits o salt ater can unction as depositional re or terrestrial nitroen and carbon bound or the ocean. onterm trends indicate that salinit is miratin substantiall landard in man reions and is predicted to do so in comin decades in the aciic orthestern . hanin hdroloic and salinit reimes at the intersection o rierbasin and coastalnearshore processes in turn can produce shits in soil biochemistr. he impacts o these shits on and cclin depend in lare part on microbial communit unction. n this stud e souht to discern the sensitiit o coastal etland soils to altered hdroloic and salinit reimes. ur oerarchin oal as to uanti shits in H 2 and 2 lues rom a tidal reshater etland communit in response to salt ater intrusion. nderstandin the eects o larescale disturbances on the unction o coastal etlands as reenhouse as sources or sins is critical to improin processrich models o climate chane eects. hanes in poreater salinit are epected to oerhelm the salt tolerance o some plant and microbial communities. Hoeer the manitude and time rame o responses to saltater intrusion b soil microbial communities in these ecosstems as measured b reenhouse as lu are not ell studied. e aimed to address this nolede ap throuh hpothesisdrien eperiments on intact soil cores in the ambient coastal enironment.

ased on the ariable tolerance o microbial taa to salinit e hpothesied that sudden chanes in salinit reimes ould aect communit unctions includin the emission o reenhouse ases. e used a coupled cait rindon spectrometer and automated chamber sstem suitable or samplin rapidl chanin conditions. e conducted a reenhouse as lu eperiment outdoors at a coastal laborator in three month phases. ach phase continuousl sampled si 1 cmdeep b 1 cmdiameter tidal reshater etland soil cores on an hourl basis. e compared reshater and ppt salinit treatments under saturated and tidal periodic saturation conditions in dierent combinations durin the phases.

e ound that the contribution rom 2 as suppressed b the saturated treatment compared to the tidal treatment and the tidein condition as suppressed compared to the tideout condition in both the ppt treatment and reshater treatment. hen the tide as out the ppt treatment suppressed the eolution o as 2compared to the reshater treatment. he ppt condition suppressed as H compared to reshater under saturated conditions. urther tidal inundation suppressed lu as H compared to saturation and both neatie and positie lues occurred. he contribution o rom 2 spied as the tide shited in the ppt and the reshater treatments hoeer the manitude as loer in the reshater treatment.

hese results indicate that under a sea leel rise scenario saltater inlu at a ppt leel ould suppress eoled as H and to a lesser deree 2. aturation hen achieed ould also suppress eoled as 2. Hoeer the contribution o rom 2 ould increase ith salinit spiin durin tidal luctuation.

m orde is a senior research scientist ith more than 20 ears o eperience in etland ecolo. he has etensie eperience ith etland research particularl ealuatin the eects o arin enironmental conditions on etland response.

ontact normation m . orde Marine ciences aborator 12 . euim a d. euim 2

47 12th International Symposium on Biogeochemistry of Wetlands

Katie Bowes c ii o . i epartment o ceanoraph and oastal ciences ouisiana tate niersit aton oue

cess loadin o phosphorus and nitroen into auatic sstems leads to deradation o ater ualit and diminished important ecosstem serices. n the orthern ul o Meico M ecess and loadin has led to a seasonall present hpoic area ith less than 2 m 2 in bottom aters approimatin 26000 m2 in sie in 201. seuential etraction method as perormed on suricial sediments rom ie dierent coastal and shel sites as a unction o distance rom the Mississippi ier mouth in the M. o better uanti temporal ariabilit in distribution and speciation samples ere collected durin both lo uust and hih Ma rier lo reimes. euential etraction techniues hae been successul in separatin pools o into echaneable or loosel sorbed e uthienic etrital and ranic. reliminar analses suest that Ma concentrations are siniicantl hiher than uust concentrations. here as no consistent trend in concentration ith sediment depth. he 010 cm sediment interal as characteried b a mean moisture content o . 12.1 and a mean bul densit o 0.2 0.2 cm. ontinued monitorin o sediment speciation and cclin is critical or understandin coastal eutrophication and inormin eectie nutrient manaement strateies to combat hpoia.

atie oes is a raduate esearch ssistant in the etland and uatic ioeochemistr ab at ouisiana tate niersity. Katie’s research ocuses on phosphorus speciation and distribution in coastal ouisiana as a unction o seasonal hpoia.

ontact normation atie oes ouisiana tate niersit est arer ld. aton oue 00

48 April 23-26, 2018 | Coral Springs, Florida, USA

Joshua L. Breithaupt1 os . o is . rs1, D risi . rs iesity eta ia a iesity t ia t. etes te ss iesity s a e t aes staia

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49 12th International Symposium on Biogeochemistry of Wetlands

Paul E. Brewer . ric oil

. liir . rioli –

50 April 23-26, 2018 | Coral Springs, Florida, USA

Stephanie T. Castle, Eliška Rejmánková

other enzymes. Using Spearman’s Rank correlation coefficients, we found a significant positive correla

51 12th International Symposium on Biogeochemistry of Wetlands

Lisa G. Chambers, avalen einmlle, n an, an on, o one, Rame Re University of entral lorida, rlando, , US hinese cademy of Sciences, hangchun, hina uian ormal University, uzhou, hina University of lorida, ainesville, US

Sea level rise eposes many freshwater coastal wetland soils to gradual increases in salinity, as well as more freuent salinity pulsing events. he uniue chemistry of seawater has the potential to significantly alter soil microial communities, physiochemical properties, and iogeochemical cycles. f particular concerns is the effect of saltwater intrusion on soil organic caron cycling, ecause the alance etween inputs and losses are a key factor in determining the likelihood a coastal wetland will e ale to accrete vertically at a pace sufficient to persist with rising sea levels. dditionally, the soil organic matter also contains vast stores of nutrients e.g., nitrogen and phosphorus that may undergo accelerated mineralization following saltwater introduction. Several laoratoryscale studies have een conducted on freshwater wetland soils from varying locations in attempt to etter understand the shortterm mechanistic response of soil microial community function and soil physiochemical properties to seawater additions. ey findings indicate that the intrusion of lowsalinity seawater . to ppt has the potential to significantly accelerate microial activity, resulting in increased losses of total mainly as and nitrogen mainly as , without any suppression of production. n contrast, the effects of lowsalinity seawater on phosphorus and dissolved organic production in freshwater wetland soils are variale and sitedependent.

r. hamers is an ssistant rofessor in the iology epartment at the University of entral lorida and the rinciple nvestigator of the uatic iogeochemistry a. er research focuses on iogeochemical cycling in wetlands and coastal ecosystems with a particular interest in responses to perturations e.g., sea level rise, storm surge, restoration, vegetation community shifts.

ontact nformation isa hamers, University of entral lorida, ira r., rlando, ., US

52 April 23-26, 2018 | Coral Springs, Florida, USA

ian eeke, anne okin, iael aon, oi ami, Eik illekov, an Jeffrey P. Chanton1 epartment of arth, cean, and tmospheric Science, lorida State University, allahassee, , US, epartment of hemistry and iochemistry, lorida State University, allahassee, , US epartment of iology and the ale iving with akes entre, aurentian University, Sudury, , anada School of orest Resources and nvironmental Science, ichigan echnological University, oughton, , US U.S. orest Service, orthern Research Station, oughton, , US

eatlands contain around one third of the gloal soil caron, with the greatest concentration occurring at high latitudes, and a warming climate is predicted to impact the caron alance within these systems. hile high latitude peatlands seem to e the most sensitive to climate change, a gloal picture of peat organic matter chemistry is reuired to improve models of greenhouse gas emissions fueled y peatland decomposition. s a part of the loal eatland icroiome roect , around samples of peat were measured with ourier transform R to eamine the organic matter functional groups of peat. arohydrate and aromatic content, as determined y R, are useful proies of decomposition potential and recalcitrance, respectively.

ur research uestions were as follows ill carohydrate content of peatlands near the euator e lower than those at high latitudes ill aromatic content of peatlands near the euator e higher than those at high latitudes hat does the distriution in peat chemistry mean in terms of a warming climate

Relative to higher latitudes, lower latitude peat had less carohydrate content and more aromatic content ased on R spectra peak heights. he larger carohydrate content of high latitude peatlands indicates greater potential for laility and resultant mineralization to form greenhouse gases, whereas the composition of low latitude peatlands is consistent with their apparent staility. ther variales such as elevation and p can more directly influence carohydrate and aromatic content, ut the overall trend shows peat at lower latitudes is more decomposed than peat at higher latitudes. he comination of low carohydrates and high aromatics at warmer locations near the euator could foreshadow the organic matter composition of high latitude peat transitioning to a more recalcitrant form with a warming climate.

eff hanton is on the faculty at lorida State University in the epartment of arth cean and tmospheric Science. e is interested in the controls on organic matter preservation and laility.

ontact nformation r. effrey hanton, lorida State University, allahassee , US

53 12th International Symposium on Biogeochemistry of Wetlands

Samantha K. Chapman, lenn olen, eae an, elea aeo epartment of iology, illanova University illanova, , US epartment of iological Sciences, ndian River State ollege, , US

limate change alters coastal wetland ecosystems oth directly through chronic warming and indirectly via influences on plant community structure. n terrestrial ecosystems, oth warming and climateinduced plant changes have een shown to alter root dynamics, decomposition and microial functioning. hough we are beginning to understand the implications of mangrove encroachment for marsh ecosystem processes, we don’t yet understand how processes such as elowground caron storage and soil elevation maintenance will respond to direct climatic warming. hough drivers of oth mangrove encroachment and soil elevation differ across the gloe, eamining the effects of warming on plant and microial processes can potentially advance our mechanistic understanding of the coastal elowground environment.

Using two passive warming eperiments, we measured aoveground plant growth, root dynamics, decomposition rates, caron stocks, and microial communities with the goal of eamining future wetland soil elevation maintenance and caron storage. arming chamers, placed at the ecotone etween mangroves and salt marshes in eastern lorida, warmed the air temperature y an average of . armed plots produced two times more salt marsh iomass and taller mangroves with more leaves. angroves also rapidly took over the marsh in plots that were warmed. e were surprised to find that warming seems to decrease salt marsh root iomass, particularly in deeper sediments. onversely, warming dramatically increased root iomass in areas where shruy mangroves had invaded marshes. hese warminginduced increases in root iomass resulted in increased relative surface elevation in less than two years. espite these increases in organic matter due to the interaction of warming and mangrove encroachment, mangrove roots can decompose more rapidly than marsh roots after one year. urther, mangrove encroachment also changes the soil microial community structure y decreasing the amount of oligate anaeroic microes, potentially indicating that soil oygen availaility is higher, which may also alter organic matter decomposition rates. s oth mangrove encroachment and warming continue to take hold in some coastal ecosystems, we need a etter understanding of the alance of root iomass accumulation and organic matter decomposition, which will e important for maintaining wetland resilience to inundation.

Samantha hapman is an ecosystem ecologist and the nne uinn elsh onors faculty fellow at illanova University. Sam uses gloal change eperiments and mechanisms la techniues to investigate coastal and terrestrial forest ecosystems.

ontact nformation r. Samantha hapman, illanova University, illanova,

54 April 23-26, 2018 | Coral Springs, Florida, USA

Bahram Charkhian outh lorida ater anagement istrict, est alm each, ,

he purpose of the iscayne ay oastal etlands proect is to contribute to the restoration of iscayne ay and adacent coastal wetlands as part of a comprehensive plan for restoring the south lorida ecosystem he proect redistributes freshwater from eisting point source canal discharges to coastal wetlands adacent to iscayne ay providing for a more natural and historic overland flow to remnant tidal crees he proect will improve the ecological function of saltwater wetlands and the nearshore bay environment by improving salinity conditions for fish and shellfish nursery habitat

he ater esources eform and evelopment ct of authoried the iscayne ay oastal etlands roect lements of these proects have been constructed and operated over the last years hase is composed of three components eering state, utler etlands and omponent n advance of congressional authoriation, the outh lorida ater anagement istrict constructed the eering state omponent and installed culverts for the omponent y epediting the completion of these proect features, hydrologic improvements and environmental benefits are already being realied

omparison of ecological monitoring data collected during the last si years with previous baseline data, indicates that the proect is trending towards success here is improved water uality to the bay as fresh water is redirected from canals to wetlands via the ulverts and the eering state omponent dditionally, point source fresh water discharges have been reduced onitoring results clearly demonstrate improved hydrologic conditions in response to operation of the eering state pump station he nterim electric pump was installed in arch , and became operational in ugust here were no eceedances of lass marine water criteria for eering state omponent and ulverts

egetation within the vicinity of eering state lowway is responding to improved hydrology demonstrated by dieoff of upland vegetation, emergence of wetland species and epansion of sawgrass urface water salinity decreased to in response to the pumping of fresh water from the eering state omponent ump tation into the historic remnant wetlands in the vicinity of utler ree roundwater salinity near the eering state low way also responded to the input of fresh water from into the historic remnant wetlands, salinity decreased to less than awgrass mapping of acres in the iami adeounty reserve wetlands was performed in and uring that period of time there was a acre increase he istrict recently, recommended changes in operation of eering state ump station using a cfs pumping and regime that will improve the condition for developing natural wetland hydroperiods and other recommendations that would improve sheet flow across wetlands

his presentation will focus on recent restoration benefits in the iscayne ay oastal etland roect

ontact nformation ahram harhian, outh lorida ater anagement istrict, un lub oad, est alm each,

55 12th International Symposium on Biogeochemistry of Wetlands

– Guangcheng Chen1, on e, nan en, an , aii en,, in an an in en hird nstitute of ceanography, tate ceanic dministration, iamen, uian, hina iamen niversity, iamen, uian, hina

angrove rehabilitations have been widely carried out to compensate the loss of mangrove wetlands around the world he restoration practices also contribute to substantial benefit for climate change mitigation through blue carbon seuestration by the created mangrove ecosystem uitable habitat conditions, eg tidal elevation, substrates and salinity, and dense plantation as well would improve the biomass carbon seuestration n hina, tree mangrove species have been widely used for plantation due to considerations lie availability of propagules, cost efficiency and rapid canopy coverage lthough the treemangrove forest has faster accumulation in biomass organic carbon stoc, a recent study in outh uian, hina showed that the dwarf eiea onilam shrub has more a substantial effect on the soil accumulation than that of a anelia oovaa forest with similar age years and plantation density, and resulted in higher soil stoc hese suggest that the variability of mangrove species in carbonseuestration benefit should be considered in the mangrove restorations

n another study in in outh uian, hina, we also recorded increases in soil content and ecosystem stoc including the biomass and top meter soil stocs with forest age in the restored oovaa forests he ecosystem stoc was ha at the nonvegetated mudflat as beginning stage of the restoration, and increased to ha at the year site and to ha at the year site owever, accumulation of in the soils leaded to increases in carbon gas emissions from the soil to the atmosphere he warming effects of two trace gases, nitrous oide and methane , would partially reduce the carbon seuestrated by the ecosystem in the older forest

oreover, anthropogenic nutrient inputs would enhance the greenhouse gas emissions from mangrove soils or instance, discharge of washout wastewater from adacent shrimp ponds rich in organic matter and nutrient into mangrove wetland had no significant effect on soil carbon dioide flu however, the discharge immediately leaded to intensive increase in soil flues of and , which could be respectively up to ~100 μmolN m h and ~100 μmolCH m h , while the flues at the nondischarge site were <1 μmolN m h and <20 μmolCH m h , respectively lthough the and flues faded with sampling time, the enhancement effect lasted for days e therefore suggested that warming effect of greenhouse gas emissions form mangrove soils, especially those subected to eogenous nutrient input would reduce the benefit of mangrove restorations in carbon seuestration and it is therefore essential to reduce discharges from various anthropogenic activities, particularly nutrientrich wastewater

r hen is specialied in mangrove ecology and his research interests mainly focus on the understanding the development of ecosystem structure and functions of restored mangrove wetlands, and the potential of mangrove wetlands to climate change mitigation

ontact nformation uangcheng hen, hird nstitute of ceanography, tate ceanic dministration aue oad, iamen, uian, , hina

56 April 23-26, 2018 | Coral Springs, Florida, USA

Hongjun Chen, een oin, avi neon outh lorida ater anagement istrict, est alm each, ,

issolved oygen availability in streams influences the growth, distribution, and structural organiation of auatic communities and thereby impacts the metabolism and productivity of auatic ecosystems s an indicator of water quality and the ecosystem’s response to hydrologic and physical restoration, DO is one of the important performance measures being used to evaluate the status and success of the issimmee iver estoration roect ince completion of hase construction in , declines in concentration in the hase area resulting in hypoic – mg and anoic mg levels have occurred at least annually in the issimmee iver sag events are defined as starting when declines below mg and ending when rises above mg our sag events observed in 2016−2017 were anoxic events in which the concentration of DO declined below 1 mg/L. hey were therefore potentially lethal to entrarchids bass and other sunfish and would be epected to have the potential to inhibit recovery of entrarchid populations hree of the anoic events were associated with increases in flow from the river’s headwaters lakes caused by heavy rainfall in the Upper Kissimmee Basin; the fourth event occurred in early wet season and may have been initiated by preevent rainfall associated with runoff from the floodplain he effects of rapid increases in upstream discharge on photosynthesis and respiration, both alone and in concert with local rainfall, can drive and sustain hypoicanoic conditions in the issimmee iver e hypothesie that most declines occur when increases in flow and rainfall reduce light penetration by increases in water depth and turbidity physically disturb photosynthetic auatic biota and mobilie nutrients from soils and organic debris as dissolved organic , , and , both on the inundated floodplain and in runoff from the floodplain to the river channel when flow is within channel ban such influes of nutrients can increase the rate of biochemical oygen demand due to higher respiration aily data from monitoring stations in the issimmee iver are analyed in relation to upstream discharge and local rainfall otential mechanisms for these sag events are discussed, as are adaptive water management actions that may reduce the severity andor duration of sag events

r hen is a senior scientist with more than years of eperience designing and implementing wetland research proects e has etensive eperience with the verglades restoration, stormwater treatment wetlands, and issimmee iver estoration roect

ontact nformation ongun hen, pplied cience ureau, un lub d, est alm each, ,

57 12th International Symposium on Biogeochemistry of Wetlands

Michael J. Chimney outh lorida ater anagement District, est alm Beach, , U

he lorida verglades is a vast oligotrophic freshwater wetland that contains a variety of habitat types eg, openwater slough, wet prairie, sawgrass marsh, tree island and dominates the landscape of south lorida his unique ecosystem is of immense ecological importance on both a national and international level gricultural and urban development over the last years has reduced the presentday size of the Everglades (≈ 960,000 ha ac to only percent of its original etent n addition, portions of the verglades have eperienced eutrophication primarily due to the inflow of phosphorus rich runoff coming chiefly from the verglades gricultural rea he verglades tormwater reatment reas s are a comple of five large constructed treatment wetlands operated by the outh lorida ater anagement District District that are integral components of tate and ederal efforts to protect what remains of the historic verglades he s have been in operation for the past years, over which time their treatment area has increased from an initial single wetland in 1994 (≈ 1,500 ha [3,800 ac]) to today’s wetland complex that encompasses ≈ 23,000 ha (57,000 ac tormwater runoff is routed into the s through a system of canals, pump stations and other water control structures that are part of the District’s floodcontrol infrastructure Because the verglades is limited, the primary function of the s is to reduce the total concentration in runoff to levels that are protective of the downstream verglades ecosystem

reatment performance requirements mandated by the operating permit are based solely on reducing outflow concentrations owever, these wetlands process other constituents to varying degrees or eample, the periodofrecord O removal of and total nitrogen for all combined is and , respectively he s routinely reduce outflow concentrations of inorganic and species OO, , O to method detection levels while they are much less effective at sequestering dissolved organic and fractions he s also process other water quality parameters, such as iron, calcium and sulfate he primary longterm storage sink in the s is the accretion of algal, microbial and plant biomass into the wetland sediments oncern has been voiced that sediments may become saturated over time and gradually lose their ability to act as a storage sink leading to a reduction in treatment performance Data presented at indicated no longterm decline in removal in the s his presentation will build on this analysis and evaluate whether there is any evidence that the s over their O have eperienced loss of treatment for other constituents based on an eamination of cumulative inflow mass versus cumulative retained mass curves and annual areal loading rates

BO Dr himney is an applied limnologist with years of research and monitoring eperience in a variety of freshwater habitats e holds professional certifications from , and mployed at the District since , Dr himney has participated in numerous proects involving the s and other water treatment technologies

ontact nformation Dr ichael himney, outh lorida ater anagement District, un lub d, est alm Beach, , U

58 April 23-26, 2018 | Coral Springs, Florida, USA

Gail L. Chmura, ee vanenne, an ollene, oma oe, im ia cill University, ontreal, , anada Bryn awr ollege, Bryn awr, , U University of uelph, uelph, O, anada

alt marshes are recognied for their eceptional rates of storage of organic carbon termed “blue carbon”. They have served as blue carbon sinks for millennia, in some locations accumulating meters of carbonrich soil On many coasts around the world marshes have been subected to drainage, primarily for agriculture and presumably causing significant losses of stored organic carbon Despite the geographically etensive nature of this practice the rate or etent of blue carbon loss with marsh drainage has been documented in few areas e are aware of research quantifying rates of loss from salt marsh drainage at only three sites enice agoon, taly; the acramento Delta, alifornia, U; and the unter stuary, ew outh ales, ustralia n fact these were the only studies available to calculate default emission factors prepared for the upplement to the uidelines on ational reenhouse as nventories etlands

he sites where marsh drainage was eamined all have mild winter temperatures thus are likely to have more rapid annual decomposition rates and not be representative of losses from marshes on colder coastlines, such as the east coast of anada reeing winter temperatures on the anadian coast should reduce carbon losses from its drained marshes o assess carbon loss of marshes in this region, we eamined short and longterm carbon loss in drained marshes along the t awrence iver, uebec ong term carbon loss was calculated by comparing the carbon inventory of flooded and drained marshes e simulated short term carbon loss that would occur immediately upon drainage, by deploying decomposition bags over a growing season e found that short term decomposition of belowground biomass in the drained marsh soil was nearly twice that of the undrained soil ong term average rates of loss in drained soils was nearly g m yr lower than the default emission factor available to estimate greenhouse gas emissions with land use change

BIO: Dr. Chmura is a professor in McGill’s Department of Geography. She has greater than 25 years’ experience researching tidal wetlands. er research has been focused on greenhouse gas flu, carbon storage, sea level change and coastal squeee, as well as paleo environmental reconstruction of wetlands

ontact nformation Department of eography, cill University, herbrooke t , ontreal, , B, anada

59 12th International Symposium on Biogeochemistry of Wetlands

Mark I. Cook, an eman, iael anna, ia ei South lorida ater Management District est alm Beach S

utrient cycles and loads are important determinants of the ecological character of wetland ecosystems and hae been greatly affected by human actions. Despite the importance of nutrients in wetlands the aried linages between nutrient cycles and wetland fauna hae receied scant attention. ere we explore some of these linages in the context of the ecology and restoration of the lorida erglades an oligotrophic wetland ecosystem that has suffered half a century of anthropogenic phosphorus inputs extensie alterations to its hydrology and declines in its populations of wildlife.

irst we examine how leels indirectly affect fauna through impacts on habitat structure. inputs to the oligotrophic erglades hae caused a regime shift from the original sawgrass laim jamaiene ridge and slough matrix to ha of dense emergent egetation dominated by cattail a spp. ey constraint on ecosystem function is the high density of the emergent egetation which results in net heterotrophic production and limits access to wildlife. e assessed aian and auatic faunal responses to a multiyear ecosystemscale experiment that tested the effectieness of chemical treatment and controlled burning as a management strategy to accelerate the recoery of a eutrophic erglades marsh. The management strategy resulted in a significant change in the proportion of openwater habitat dissoled oxygen concentrations primary productiity and auatic metabolism. The aerage combined biomass of small fish and crustaceans did not differ significantly between dense emergent egetation and created openings but community composition did differ between treatments with crayfish dominating in emergent egetation and small fish and grass shrimp dominating in open water. ish had greater whole body C and contents low C: compared with the two crustaceans. lthough the management action did not alter the uantity mg carbon m2 or energy cal m2 of fauna the resultant shift in composition did correspond with an increase in the nutrient uality mg m2 and low C: of fauna. The faunal community composition of the created openwater habitats in the eutrophic erglades was similar to that of oligotrophic erglades howeer density and biomass were significantly lower for the oligotrophic region. Moreoer fish and decapod crustaceans from the oligotrophic region were smaller and had lower whole body contents than organisms from eutrophic regions. This suggests that consumers are limit in the oligotrophic erglades. These difference in the production and uality of prey fauna between the eutrophic openings and oligotrophic erglades led to changes in the composition and abundances of waterbird communities between the areas as well as important differences in the mechanisms driing prey aailability.

Second we explore some of the mechanisms by which wildlife impact nutrient cycling in the erglades. or example fish and wading birds play a ey role in fertiliing arious wetland habitats by transporting and concentrating nutrients ia their feces nesting and decomposing bodies. e discuss the implications of the dramatic loss of wildlife populations to erglades nutrient cycling as well as the potential impacts of recoering these populations through hydrologic restoration.

BIO: Dr. Coo is an aian ecologist with 22 years of experience studying waterbirds. is research focuses on how wetland processes such as hydrology and nutrient runoff affect wading bird foraging and reproductie success.

Contact Information: Mar I. Coo erglades Systems ssessment Section pplied Sciences Bureau South lorida ater Management District est alm Beach S

60 April 23-26, 2018 | Coral Springs, Florida, USA

Jennifer Cooper, imo an an amia ao niersity of lorida erglades esearch and ducation Center Belle Glade S

hosphorus enrichment has been identified as a contributor to the diminished water uality and negatie ecological changes occurring in the erglades ational ar in southern lorida. Discharges from the erglades gricultural rea a former sawgrass marsh drained for agricultural use in the early s hae been identified as a source of eleated leels in the erglades. Discharges from the contain approximately 5 particulate predominantly from plant detrital material with the remaining in the more reactie dissoled form. Calcium has been assumed to be the predominant element that to precipitate with phosphorus and remoe from the mobile phase howeer direct comparatie experiments of the potential for other metals Mg e l Mn to precipitate under conditions hae not been tested. dditionally floating auatic egetation which can alter p and oxidationreduction potential has not been examined for its potential impact on different mineral precipitations with . To uantify the different contributions of egetation and minerals to phosphorus remoal we performed a greenhouse leel study comparing the remoal of from canal water in the presence of Ca Mg e l and Mn minerals in 25 drums. Three replicates of each mineral were seeded with while another three replicates were grown without . ew canal water was introduced to the drums eery two wees with weely or biweely collection of phosphorus species p oxidationreduction potential mass of accumulated detrital matter mass loss of mineral and analysis of water Ca Mg e l and Mn concentrations. Calcium precipitation with total was highly influenced by the presence of with mean total remoal increasing from . with coerage to in the absence of . The magnesium treatment was not significantly affected by coerage with mean total remoal of with and without . The iron was also not significantly affected by the presence of aeraging 2 with and 2 without . The presence of significantly reduced total remoal in the manganese treatment from without to . emoal of total under aluminum treatment was also affected by the presence of with total remoal increasing from with to 2 without . Our results indicate that Mn may play an important role in remoal in the along with Ca and warrants further research.

BIO: Dr. Cooper is a postdoctoral research associate woring on reduction of phosphorus discharges and soil subsidence in the erglades gricultural rea of Southern lorida.

Contact Information: Dr. ennifer . Cooper esearch group of Dr. Samira Daroub 2 Canal St Belle Glade

61 12th International Symposium on Biogeochemistry of Wetlands

R. Corstanje1, aaka, vano, an ieo Cranfield niersity Cranfield nited ingdom 2South lorida ater Management District est alm Beach S

Data analytics of large datasets are increasingly being used in the enironmental studies to explore underlying factors structures and driers which are not immediately obious. The combination of remote sensing large scale sampling and sensors has resulted in larger datasets becoming more prealent in enironmental studies including wetlands. ere we apply these methods to the Stormwater Treatment reas STs. The STs were constructed along the northern boundary of the erglades as engineered ecological systems designed to retain from water flowing into the erglades. This research focused on a large data set from 22 to 2 from STs using a pattern seeing b confirmatory modelling approaches and c spectral decomposition methods to determine the stability or resilience of the systems. The spatial analysis found common spatial patterns forming onal patterns of distribution that may increasingly align with the predominant flow path oer time. indings also indicate that the primary driers of the spatial distribution of in many of these systems relate to soil characteristics. The results also suggest that coupled cycles may be a ey component of these systems i.e. the moement and transformation of is coupled to that of nitrogen . The spectral decomposition of outflow data was then used to determine the stability and resilience of these systems illustrating how these methods can be used describe underlying data structures and their stability oer time.

Contact Information: rof. onald Corstane Cranfield niersity Cranfield nited ingdom

62 April 23-26, 2018 | Coral Springs, Florida, USA

Kristofer Covey ale School of orestry and nironmental Studies ew aen CT S

orests seuester .. g C yr an ecosystem serice worth hundreds of billions of dollars annually. ollowing the CO2 meeting in aris an international consensus emerged: the protection and expansion of forests is necessary to limit global warming to less than 2C . The fact that trees emit methane C howeer is underappreciated despite the potential for these emissions to significantly offset the climate benefit of forest CO2 seuestration. ere we determined C concentrations in broadleaed and coniferous trees in paired young and mature stands across 2 sites in the eastern .S. finding C concentrations as high as . i.e. 5times atmospheric concentrations. The highest concentrations were found in older broadleaed trees during the growing season 22.2. ppm with eergreen species showing only modestly eleated concentrations 2.. ppm. Diffusion modelling suggests that these mature broadleaed trees in particular are a source of C to the atmosphere a result confirmed using chamber methods to directly measure C efflux through the bar of liing and newlyfelled trees. sing the year global warming potential G for C we estimate that this Cproduction pathway may reduce the collectie climate mitigation alue of the eastern forest by . oweer because concentrations ary with species identity and stand age the climate serice benefit of some forest stands could be reduced by 25 or more.

BIO: Dr. Coey is the ead Scientist at the cross igh lains Stewardship Initiatie and a ecturer in orest Dynamics at the ale School of orestry and nironmental Studies. is research focuses on aniin e l o meane om livin ee ia in i microbial methanogenesis.

Contact Information: Dr. ristofer Coey ale niersity School of orestry and nironmental Studies ew aen CT

63 12th International Symposium on Biogeochemistry of Wetlands

Hayley Craig1, lka elle, Ria ae an ennie Ronee School of arth and nironmental Sciences The niersity of Manchester Manchester nited ingdom 2Smithsonian nironmental esearch Center dgewater MD S School of Science and the nironment Manchester Metropolitan niersity Manchester nited ingdom

Mangroe soils contain a dierse community of microorganisms that perform a ast array of ecological functions. s mangroes are naturally subect to freuent changes in enironmental conditions it is thought that high bacterial diersity in mangroe soils could ameliorate detrimental impacts associated with enironmental disturbance. In mangroe forests of the ew orld where plant species diersity is low intraspecific genetic diersity of plants could play an important role in controlling the diersity of mangroe associate species including soil microorganisms. A loss of plant genetic diversity can reduce a habitat’s ability to cope with disturbance and if this also leads to a reduction in microbial diersity the problem could be compounded. Changes in microbial community composition may also hae implications for nutrient cycling in these systems. ew studies hae used methods to assess microorganism diersity and composition in mangroe soils and none that we now of hae looed at whether this is lined to plant genetic diersity. This study aims to assess how diersity and composition of soil microbial communities are affected by plant genetic diersity plant species identity and habitat one.

eaf samples were collected from Riooa manle and viennia eminan across different habitat ones at two sites in lorida for microsatellite analysis using marers deeloped for this geographic region. D was extracted from soil collected beneath each of the plants sampled and terminal restriction fragment length polymorphisms T and Illumina seuencing were used to assess soil microbial community diersity and composition. dditional soil ariables including p temperature moisture and nutrient content were also assessed. This study provides much needed information on the composition of Florida’s mangrove soil communities and the factors that influence them.

BIO: ayley Craig is a hD student inestigating how plant genetic diersity and nutrient influence composition and function of soil microbial communities in mangroe ecosystems.

Contact Information: ayley Craig School of arth and nironmental Sciences The niersity of Manchester Michael Smith Building Doer Street Manchester nited ingdom M T

64 April 23-26, 2018 | Coral Springs, Florida, USA

Stephen Crooks1, om i, ae eol, lana enal, ame olmi, ian ole, aik eonial, en , iana onie, an eei ilvestrum limate Associates ill alley A A nvironmental rotection Agency ashington ashington A AA harleston ffice for oastal anagement harleston A mithsonian nstitute mithsonian nvironmental esearch enter dgewater A Florida nternational niversity nvironmental esearch tr. ept. iological ciences iami F A The ature onservancy ethesda A AA limate rogram ffice ilver pring A

The Inventory of U.S. GHG Emissions and Sinks’ (nveno chapter on and se and se hange and Forestry F reports stoc changes and emissions of and from forest management and other land uselanduse change activities. ith the release of the upplement to the uidelines for ational nventories etlands elan lemen the nited tates has begun woring to include emissions and removals from management activities on coastal wetlands

To support the A AA formed an interagency and science community group i.e. oastal etland arbon oring roup tased with conducting an initial Tier baseline assessment of emissions and removals associated with coastal wetlands using the methodologies described in the etlands upplement.

The .. process of building the inventory reporting years has draws on freely available andsat data countryspecific soil carbon stocs data derived from peerreview literature and approaches for calculating carbon stoc change. Activity data in the form of land cover and landuse change is derived from a series of surveys collated under the AA oastal hange Analysis rogram A. istribution of organic soils was derived from national soil survey databases. uantification of etent of remaining coastal wetlands and former wetlands now converted to other land use categories are ey elements of the analysis. These results were derived from AA oastal hange Analysis rogram A a national A dataset to provide topography coupled with analysis of tide station data to map tidal water incursion and etent of organic soils derived from oil urvey mapping. Tier i.e. countryspecific level subnational climate one estimates of carbon stocs including soils along with carbon seuestration rates and methane emissions rates have been developed from literature.

Future opportunities to improve the coastal wetland estimates include refined analysis of reference soil carbon stocs and uncertainty analysis refined uantification of methane emissions from wetlands across the salinity gradient including mapping of this gradient and from impounded waters uantification of impacts of forestry activities on wetland soils emissions and removals on forested tidally influenced and palustrine wetlands on coastal land areas the fate of carbon released from eroded wetlands and the etent of seagrass along with the emissions and removals associated with anthropogenic impacts to them.

r. roos is a wetland scientist with more than years of eperience planning designing and implementing wetlands restoration proects. e serves as the ource ead to the A for oastal etlands.

ontact nformation tephen roos eminary rive ill alley A

65 12th International Symposium on Biogeochemistry of Wetlands

Emily M. Dangremond1, lka elle oosevet University Shamr I US Smithsonian Environmenta esearh enter Edeater US

Coastal wetlands have experienced regime shifts through major changes to their foundational species. In Florida’s temeratetroia eotone here manroves and satmarshes meet manroves have inreased in andane sine . Hoever eteen and manrove haitat eanded and ontrated indiatin that manrove eansion may not e soey attritae to reent imate hane t may e an onoin dynami roess of eansion and ontration. In this stdy e investiate the drivers of reime shifts eteen manrove and satmarsh in the temeratetroia eotone. e omined historia reords and imaery ith fied oservations and revios remote sensin anaysis to evaate the hyothesis that are infreent distranes namey freee events and hrrianes are the main drivers of this reime shift.

In the asene of freee events manroves soy oy the eotone y shortdistane disersa as roaes diserse short distanes from the arent ant and otomete satmarsh ants. ied data on roae disersa fooin Hrriane Irma in shoed that roae density an inreased from roaesm in the asene of a hrriane to . roaesm after a hrriane. This sorts the idea that hrrianes an inrease the seed of a reime shift y ondistane disersa artiary hen they hit in ate Setemer to eary toer and trak soth to north aon the oast of orida. anroves had a raid eansion fooin hrrianes in and and a rane ontration fooin freees in and . rin the s there ere for severe freees that revented manrove roth. Severe freees and hrrianes are are infreent distranes that shifted the fondationa seies in the manrovesatmarsh eotone eteen and . These distranes ikey ased simiar reime shifts in earier time eriods hih is sorted y historia aonts of manrove resene in the eotone and aonts of manrove dieoffs fooin freee events. Historia reords datin to the s indiate that manroves may have orred in their resent oations at revios eriods. Under the rrent reditions for imate hane e eet a hane to this natra osiation de to armin temeratres and hanes to hrriane freenyintensity oth of hih favor manrove eansion.

I Emiy anremond has een an assistant rofessor at oosevet University sine st . She started stdyin manroves as a h stdent at the University of aifornia erkeey here she stdied elliiea iooae in anama. s a ostdo she stdied reoios rerodtion in manroves at their northern rane ede in orida.

ontat Information Emiy anremond oosevet University . oosevet vd Shamr I

66 April 23-26, 2018 | Coral Springs, Florida, USA

Jeroen de Klein1, i illien, in , ene aee oaa, nnelie eaa ageningen niversit ageningen he etherlands stacin iolgica de oana evilla pain adoud niversit ijmegen he etherlands

atural and constructed wetlands can emit sustantial amounts of greenhouse gasses . owever the also seuester caron in iomass and thus wetlands can e either sins or sources of e lein vd erf . he aundance and tpe of auatic vegetation in wetlands is an important factor for fluxes and the distriution among the various gasses. ere we studied the effect of floating and sumerged vegetation in a natural wetland and in an experimental setting with microcosms. Floating vegetation can affect emissions through multiple mechanisms. Firstl a dense cover of floating vegetation e.g. olla emna will generall reduce oxgen concentrations in the water column and the sediment eraart et al. thus promoting methane production. owever floating iomass can hamper gas exchange with the atmosphere which reduces the actual fluxes osten et al . speciall methane ules might e trapped in the floating roots and can e oxidised to C again efore eing emitted to the atmosphere. It is still unclear which of the contrasting mechanisms will prevail under different conditions.

e uantified emissions in different wetlands of the oana ational ar in the south of pain. as fluxes were measured in a single field campaign pril with a floating chamer and a field gas analser. ine locations were selected in separate shallow laes and wetlands with different tpes and densities of auatic vegetation. econdl laexperiments were performed in the climatic rooms of ageningen niversit. In total microcosms were prepared with different vegetation olla floating emna floating lodea sumerged or no plants all treatments in triplicate. uring wees gas fluxes of all microcosms were measured oth in light and dar times per wee.

as fluxes from the field measurements indicate that with aundant vegetation there is an uptae of C and with medium or low vegetation cover C is emitted even in datime with the highest emission in the more open waters with few vegetation. For C it showed that the highest fluxes are measured from relative open water sstems with vegetation cover. he lowest Cfluxes are oserved in shallow sstems with almost complete cover of floating and sumergedemergent vegetation. From the microcosms experiment we conclude that vegetation promotes emission of . here fluxes are almost asent in sstems without vegetation clear fluxes are measured in vegetated cosms. ighest fluxes are found in olla sstems oth for C and C. s expected C fluxes are contrasting during dar and light conditions. owever C fluxes generall do not differ etween dar and light. he level of C fluxes in the microcosms is well in line with fluxes in field conditions. verall the research suggests that the dissolved oxgen reduction floating vegetation is a stronger effect on emission than the ostructed gas exchange.

I r. de lein is associate professor at ageningen niversit. is research topics involve nutrient and caron ccling in auatic sstems including emissions. In addition he has an extensive experience in applied water ualit modelling.

Contact Information eroen de lein uatic colog and ater ualit anagement roup ageningen niversit .. ox ageningen he etherlands

67 12th International Symposium on Biogeochemistry of Wetlands

an ane, Jeroen J.M. de Klein2, nne van am etherlands nvironmental ssessment genc he ague he etherlands ageningen niversit ageningen he etherlands I Institute for ater ducation elft he etherlands

here is a growing interest in etlands for ecosstem services and iodiversit. ince two thirds of the world’s wetlands have disappeared and the loss is continuing at the same pace. The remaining wetlands are threatened hdrological changes eutrophication pollution and climate change. olic maers at oth gloal such as the amsar Convention and the C and national levels are concerned aout the resulting loss of iodiversit and ecosstem services of wetlands.

o what extent are on the gloal scale these services impaired and what solutions would e optimal to solve these prolems and to improve wetlands functioning gloal model of wetland functioning could provide insights from the iophsicalecological perspective to sustain this decision maing.

ere we present the outlines of a generic model descriing the effects of climate and landuse changes on the functioning of freshwater wetlands worldwide expressed in terms of iomass production and accumulation water resources water ualit and emissions. Core variales are water level nutrients caron and vegetation. he main processes lining these are descried in a generic wa accounting for climate ones and wetland tpes as minimall necessar for a gloal picture. he model is emedded in and receives forcings from an existing gridased gloal hdrological climate and landuse model. In this wa the impacts of climate change landuse changes and water use on wetlands functioning can e modelled. eversel the model allows to simulate the contriution of wetlands to important ecosstem services. he model is now apt for validation on datasets from varing tpes of wetlands for which we see cooperation with colleagues from different parts of the world.

I r. anse is senior auatic ecologist at and guest scientist at the eth. Inst. of colog in ageningen with extensive experience in ecological modelling of standing waters e.g. ake and woring on gloal iodiversit modelling in integrated assessment projects.

Contact Information an anse etherlands nvironmental ssessment genc he ague he etherlands .. ox he ague he etherlands

68 April 23-26, 2018 | Coral Springs, Florida, USA

CRASSOSTREA VIRGINICA Derek J. Detweiler1, i in o, ovi emaeiee, ilie oan, aiia ooman, an ani ole epartment o arth and cean ciences and enter or arine cience niversit o orth arolina ilmington ilmington nstitut niversitaire uropen de la er niversit de retagne ccidentale rest ester arine ield tation and oastal atershed nstitute lorida ul oast niversit ort ers epartment o iolog and arine iolog and enter or arine cience niversit o orth arolina ilmington ilmington

The Ten Thousand slands TT is a mangrovedominated wetland ecosstem in the southwest lorida verglades. istoricall impacted drainage and channeliation due to rampant agriculture and uran development eorts under the ederal omprehensive verglades Restoration Plan (CERP) have aimed to restore, repair, and preserve the quality of TTI’s freshwater resources. This is achieved via canal levee and roadwa removal coupled with pump station and reservoir installation or eective lood control and reshwater distriution. n order to properl guide and optimie these eorts outlines that impacts o pre and postrestoration environmental conditions on e ecologic and economic species must e determined.

n TT the eastern oster aoea viinia is a valuale resource or coastal protection haitat provision water ualit improvement and mangrove seedling propagation. The preerred diet o this iltereeding ivalve tpicall includes high ualit laile particulate organic matter such as phtoplanton rather than reractor material that ma comprise allochthonous inputs o ecess nutrients and pollutants. n this stud molecular iomarer analses using phtosterols and stale isotopes were used to trace sources o terrestriall and auaticall derived organic matter rom plant material enthic microalgae sediments and oster digestive tissue. This approach allows or the assessment o trophic resource use in viinia prior to restoration. htosterols in each o these sample tpes were used to identi possile ood sources and ood ualit to viinia diet within three hdrologicall distinct as etween wet and dr seasons.

reliminar results indicate an input o common mangrovederived phytosterols such as amyrin, βsitosterol germanicol etulin and lupeol as determined lea and sediment analses. n the wet season data rom oster digestive tissue revealed that cholesterol comprised nearl hal o all neutral lipid etracts ollowed by brassicasterol, campesterol, β sitosterol and stigmasterol. n phtol and cholesterol comprised the maorit o all neutral lipid etracts ollowed the aovementioned compounds. These results indicate that during the wet season viinia eeds mainl on autochthonous phtoplanton rather than terrestrial . This is evidenced the presence o phtoplantonderived sterols ound in diatoms prmnesiophtes and green algae.

terols present in and all other sample tpes rom the dr season will e urther discussed to predict how a restored hdrologic low regime ma impact viinia diet. ased on seasonal salinit changes it is hpothesied that the dr season will ehiit an increased aundance o marinederived phtoplanton such as dinolagellates in and viinia tissue. dditionall increased reshwater low under wetter conditions will e shown to increase primar production phtoplanton growth and enhanced utiliation o viinia hsiologicall the ailit o viinia to selectivel ilter higher ualit material even under the stress o altered hdrolog highlights the importance o inorming eorts aimed at improving overall water ualit. ence while viinia distriution has decreased in recent decades due to multiple stresses eorts continue to restore the hdrologic conditions in which the thrived.

: Derek J. Detweiler is a graduate student at the University of North Carolina Wilmington pursuing a Master’s degree in Marine cience. is research eperience and interests include iogeochemical ccling in wetland ecosstems and associated impacts o altered hdrolog on organic matter ualit and trophic resource availailit.

ontact normation ere etweiler epartment o arth and cean ciences niversit o orth arolina ilmington outh ollege oad ilmington

69 12th International Symposium on Biogeochemistry of Wetlands

Brittany Dolan1, ian ova, an Ei o lorida ulf Coast University, ort Myers, , U Naples otanial arden, Naples, , U

Rooftop gardens have een used in many ultures throughout history and while they are urrently popular in many areas of the world, they are relatively new in the United tates and rare in outhwest lorida. This study utilies two types of native rooftop gardens in Naples, lorida, a modular wetland garden system whih is not used in many situations and a eri garden system whih is more widely used. Rooftop gardens an provide many environmental enefits inluding regulating uilding temperatures, reduing uran heat islands, reduing uran storm water runoff, inreasing lifespans of roofs, lowering energy osts, inreasing loal iodiversity, and providing uran wildlife haitat. This study ompares the ailities of a modular wetland rooftop garden system, a eri rooftop garden system, and a traditional (are) roof with no rooftop garden to redue the heat flu into a residential uilding. ir temperature, rooftop surfae temperature, and heat flu were measured for eah of the threerooftop systems and then ompared. Results from this study indiate the wetland system has the greatest heat flu redution apaility ompared to the eri system and the are roof. The wetland system redued the daily movements of energy into and out of the house y ompared to the eri system and y ompared to the are roof. The results also indiate oth the wetland system and the eri system signifiantly redue the average surfae temperature of the rooftop, and redue the variation in the surfae temperatures when ompared to the are roof system. The redution in surfae temperature in the wetland and eri systems eplains the redution seen in the heat flues of oth systems. This study provides evidene that rooftop gardens have great potential to derease heat flues into uildings and redue ooling osts in sutropial limates like outhwest lorida.

Bio: Brittany Dolan is pursuing a Master’s of Environmental Science degree at Florida Gulf Coast University with research currently fousing on rooftop gardens in Southwest Florida. In 2015, she graduated with a Bachelor’s of Science in Marine Biology and Environmental Science from University of New aven, CT.

Contat Information: rittany Dolan, lorida ulf Coast University, CU lvd , ort Myers, , U

70 April 23-26, 2018 | Coral Springs, Florida, USA

Bobby G. Duersch an illiam oa Department of Chemistry and , Florida tlantic University, Boca aton, F US

hosphorus is nown to e a maor cause, in addition to nitrogen of eutrophication in water systems. can enter the environment through a variety of forms such as orthophosphate, D, sedimentrocs apatites, manure, and many other anthropogenic sources. For eample, ecessive amounts of horse manure in the C51 asin leads to high soil concentrations. high amount of leaching into water sources can then occur, leading to eutrophic conditions and large algal looms. hrough nutrient analyses and phosphorus speciation studies, we hope to determine how moves through the ecosystems that feed the Everglades, ae orth agoon and adacent water odies.

his research includes nutrient , , Fe etc. analyses of horse manure and edding and the water and soils from and adacent to canals that feed into the alm Beach Canal C51 as well as the Stormwater reatment reas Ss 1E and 1. ot only will the various forms of phosphorus DI, D, , , S e uantified through a modified edley Fractionation ut the use of 1 M uclear Magnetic esonance will detail the speciation of free orthophosphate, pyrophosphate, poly , esters etc. in order to trace and determine sources and movement. ICES Inductively Coupled lasma ptical Emission Spectrometry will e used to determine total concentration for a variety of elements, notaly metals, for uantitative support of spectroscopic techniues. ovel metal removal procedures for the cleanup of phosphorous containing samples without affecting the native forms are planned. Further research is underway investigating the ioavailaility of organic phosphorus compounds on cyanoacteria. opefully, the results of this research will aid in having stronger pollution prevention legislation enacted for restoring and protecting the Everglades. his may include locally monitored otal Maimum Daily oads MDs, new Best Management ractices BMs and thelie.

cnowledgement: his research is eing funded y an award to the senior author BD from the Everglades Foundation and that support is gratefully appreciated.

BI: Boy Duersch is a hD candidate at the Department of Chemistry and Biochemistry at Florida tlantic University. ecently, he was ust awarded a scholarship of 10,000 from the Everglades Foundation to e used in implementing plans in restoring the Everglades.

Contact Information: Boy Duersch, Dr. illiam esearch Group, Glades d, Boca aton, F, US 1

71 12th International Symposium on Biogeochemistry of Wetlands

Collin A. Eagles-Smith1, ame illake, avi aeno 1US Geological Survey Forest and angeland Ecosystem Science Center, Corvallis, , US 2US Geological Survey isconsin ater Science Center, Middleton, I, US

he health riss from mercury g contamination can e difficult to characterie ecause g accumulation in food wes and associated toicological ris is largely determined y the comple, microiallymediated methylation process that converts inorganic mercury g II to methylmercury Meg the most iologically active and toic form of g. Current techniues for uantifying the influence of environmental conditions and microial community dynamics on Meg production rates in i within natural systems are limited. raditional techniues either depend on highly manipulated eperiments, are limited to culturale taa a small portion of the microial community, or are etremely laorious, challenging measurements at scales that represent ecosystem or landscape processes.

Flow cytometry is a powerful technology for rapidly and accurately enumerating individual cells in environmental samples. It can also characterie physical and physiological traits of cells using innate cell fluorescence. riginally developed for medical applications, applications of flow cytometry for environmental research have grown sustantially over the past two decades. e eamined the potential utility of flow cytometry for etter understanding the dynamics and drivers of Meg in freshwater ecosystems through paired measurements of speciated aueous g concentrations coupled with analysis of cell density and type using flow cytometry. e collected water samples from the ells Canyon eservoir Comple along the Snae iver, ordering regon and Idaho. his is a site of current intensive research on limnological and iogeochemical processes associated with Meg dynamics. ater samples were collected from 515 discrete depths at 5 uniue locations in the comple representing a gradient in water column stratification from completely unstratified to strongly stratified. e eamined patterns in microial and algal cell counts, oth along the etent of the reservoir comple, and with depth at each location. e also assessed the relationship etween microial indices and speciated g concentrations.

reliminary results indicate that a ey inde of microial activity the ratio of highnucleic acid:lownucleic acid cells : varied sustantially among locations and with depth. Specifically, microial activity was highest at stratified sites, and especially in the turidity maimum one near the thermocline, where water density changes facilitate the accumulation of decaying algal particles. he vertical profile in : closely matched that of aueous Meg concentrations in stratified sites, ut not unstratified sites. Consistent with these preliminary findings, : ratios were strongly correlated with aueous Meg concentration, oth across and within stratified sites. hese preliminary results indicate that flow cytometry may provide a rapid, inepensive tool for understanding in i microial processes associated with Meg dynamics in auatic ecosystems.

BI: Dr. EaglesSmith is a research ecologist approimately 20 years of eperience researching factors influencing mercury dynamics through food wes and across ecosystems. e wors at the intersection of iogeochemistry, food we ecology, and toicology to provide science support for resource management decisions.

Contact Information: Collin EaglesSmith, USGS Forest and angeland Ecosystem Science Center, 200 S efferson ay, Corvallis, , 0

72 April 23-26, 2018 | Coral Springs, Florida, USA

Neal Flanagan1, Riaon, onjn an Due University, Durham, C, US

Many peatlands across the gloe are suect to regimes of regularly recurring wildfires with return intervals ranging from to 100 years. hese peatlands are typically dominated y fireadapted plant communities suggesting that wildfire is an integral part of peatland ecology rather than an anomaly. eatland wildfires are typically lowseverity events that occur in winter and spring when vegetation is desiccated, and soil moisture content is high. s a result, most peatland wildfires rapidly consume aoveground fuels without igniting the nearly saturated peat. In such fires, surface soil layers are suected to flash heating with a rapid loss of soil moisture ut little loss of soil organic matter SM. Such fires alter the chemical structure of SM, even in the absence of combustion, through Maillard’s Reaction and similar chemical processes, and through structural changes that protect SM from decomposition. his study eamines the effects of lowintensity surface fires on the recalcitrance of SM from fireadapted communities located in sutropical, temperate and suoreal peatlands. In addition, soil from a nonfireadapted eruvian palm peatland was eamined for response to thermal alteration. he timing and temperatures of lowintensity fires were measured in the field during prescried urns and replicated in simulated fires. he effects of fire on the chemical structure of SM was eamined with FI, SEM and S. Burned and unurned peat replicates were incuated at three temperatures 5oC, 15oC, 25oC for more than si months. Burned replicates initially showed higher C2, C and 2 emissions. et, within four wees emissions from the urned replicates dropped elow those of unurned replicates and remained significantly lower 1050 for the duration of the eperiment. In addition, thermal alteration significantly reduced the temperature sensitivity 10 of thermally altered peat. hermal alteration of SM resulted in a net longterm reduction in caron losses to microial respiration. Such thermal alteration of SM might e an underestimated factor influencing caron accretion freuently urned peatlands and could e gloally relevant if climate change increases fire freuency in oreal peatlands.

BI: Dr. Flanagan is a esearch rofessor with more than 20 years of eperience in researching the hydrology and iogeochemistry of wetlands.

Contact Information: eal Flanagan, Due University etland Center, Bo 02 Durham, C 20

73 12th International Symposium on Biogeochemistry of Wetlands

Rebecca J. Flock, aina iee an in aen ollege of estern daho, ampa, ,

onpoint source nutrient loading in irrigation returns contribute to ater ualit impairments in freshater sstems e inestigated a seasonall emergent etland in the transitional one beteen the ernard rain and ae oell in the oer oise Rier atershed, outhest daho e sought to characterie the ecosstem serices the etland one proides as a means of improing the ualit of the ater entering the reseroir ia runoff he ernard rain returns agricultural irrigation aste ater and has been reported to hae the highest oerall detection of contaminants of the canals and drains entering the catchment ae oell is an , acre irrigation catchment and reseroir constructed in and designated as part of eer lat ational ildlife Refuge he reseroir receies drainage from approimatel , acres and proides multiple secondar serices to the surrounding communit as an actie recreation area, breeding and migrator habitat for birds and mammals, and historic landmar

stablished oer a centur ago, the aging reseroir has eperienced increasingl eutrophic conditions, phosphate and dissoled ogen impairment, as ell as the appearance of harmful algal blooms reliminar ariations in soil and ater ualit parameters indicate that the seasonall emergent etland reduces the transport of nitrate, orthophosphate, and sediment during the irrigation season dditionall, the etlands one proides a cooling effect for the margins of the reseroir, thereb helping to mitigate some aspects of ater ualit impairment hese findings illustrate the alue of identifing management practices that support the abilit of the etlands to continue proiding its ecosstem serice, especiall in transitional ones beteen agricultural drains and the reseroir

Rebecca loc is an ssistant rofessor of hemistr and mentors undergraduate students in applied phsical and chemical inestigations of land, air, and ater resources in their communit and beond he has a bacground in gricultural and nironmental hemistr and is inested in holistic amelioration of nutrientimpaired ateras

ontact nformation Rebecca loc, ollege of estern daho, M , ast pportunit rie, ampa,

74 April 23-26, 2018 | Coral Springs, Florida, USA

Hilary Flower, ak Rain, al i,, illiam em, an eman, o one,, Rame Re, aana eekea, enie ane niersit of outh lorida, ampa, , coandMod, nc, ort ierce, , outh lorida ater Management istrict, est alm each, , hitne aborator for Marine ioscience, niersit of lorida, t ugustine, , etland iogeochemistr aborator, oil and ater cience epartment, niersit of lorida, ainesille, ,

o support erglades restoration planning, e proide screeningleel simulations of plausible soil biogeochemical responses in the freshater part of the reater erglades cosstem b to macroclimate change e used three climate change scenarios a baseline scenario of climate, and to climate change scenarios that both included arming and increase in eapotranspiration, and differed onl b rainfall either increase or decrease b ith these climate conditions as input, e used the erglades andscape Model to simulate changes to eight interactie hdroecological metrics important for biogeochemical ealuation

n the increased rainfall scenario, little changed compared to the aseline scenario, because the ere hdrologicall similar ie, much of the increased rainfall as cancelled out b increased loss from eapotranspiration he decreased rainfall scenario produced mared changes across the sstem eere ater scarcit led to diminished surface ater depths that ere detrimental in regions that are alread too dr, and beneficial in regions that are too et, here both ertical soil accumulation rate and soil accumulation rate accelerated reas near structural ater inflos ehibited loadinduced declines in surface ater sulfate concentration as ell as phosphorus content of surface ater, pore ater, and soil he area corresponding to eleated methlmercur production ris shran in areal etent and migrated closer to structural inflo points urface ater flo elocit sloed drasticall across most of the sstem

e conclude that future macroclimate changes hae the potential to produce significantl different ecological responses in the freshater remnant of the erglades, particularl if arming temperatures and increased eapotranspiration coincide ith decreased rainfall Restoration planning must tae future climate change into account

r loer is a facult researcher at the chool of eosciences ith a specialiation in ecohdrolog he studies coastal etland responses to climate change and sea leel rise using a ariet of methods including field obserations, laborator eperiments, and computer modeling

ontact nformation ilar loer, h, niersit of outh lorida, oler e, ampa, ,

75 12th International Symposium on Biogeochemistry of Wetlands

Chris Freeman angor niersit, ales,

n an inestigation of the potential strategies for enhancing carbon seuestration in natural etlands, e sho that a ariet of noel approaches hold potential as modifiers of seuestration capacit errestrial and oceanic ecosstems contribute almost euall to an estimated seuestration of about of anthropogenic carbon dioide emissions, and thus alread pla a role in minimiing our impact on the global climate f the seuestered terrestrially, the majority becomes part of the Earth’s soil carbon stores. Northern peatlands coer ust 3% of the Earth’s landmass and yet sequester 455Pg of C, or almost 1/3 of that soil carbon. eatland ecosstems are thus ell established as poerful agents of carbon capture and storage he remarable preseration of ancient organic archaeological artefacts, such as bog bodies, further attest to their eceptional preseratie properties eatlands, hae higher carbon storage densities per unit ecosstem area than either the or dr terrestrial sstems ast studies demonstrate that peatlands seuester carbon due to the inhibitor effects of phenolic compounds which create an ‘enzymic latch’ on decomposition (Freeman et al 2001, Fenner & reeman ere e sho the potential for harnessing that mechanism in a series of peatlandengineering strategies reeman et al in hich molecular, biogeochemical, agronomical and afforestation approaches, increase carbon capture and longterm seuestration in peatforming etland ecosstems ur studies consider the significance of the seuestrationenhancement approach in both natural and restored ecosstems, and in the preention of destabilisation of ancient carbon stores eg Moore et al folloing human impacts

References reeman , stle , ang n enmic latch on a global carbon store ature , enner , reeman roughtinduced carbon loss in peatlands ature eoscience reeman, , enner, , hirsat eatland geoengineering an alternatie approach to terrestrial carbon seuestration hilosophical ransactions of the Roal ociet , Moore, , ans, , age, , arnett, M, ones, , reeman, , ooier , iltshire , imin and auci, , eep instabilit of deforested tropical peatlands reealed b fluial organic carbon flues ature –

hris reeman, is ead of the chool of iological ciences at angor niersit, ith more than ears of eperience of etland enme research e has etensie eperience in studing carbon ccling, the influence of etlands on drining ater ualit, and greenhouse gas emissions

ontact nformation hris reeman, etland Research roup, chool of iological ciences, angor niersit, angor, ales, ,

76 April 23-26, 2018 | Coral Springs, Florida, USA

Masanori Fujimoto, an en, Re omia an Rame Re niersity of Florida, ainesille, F,

ater quality and quantity are global issues we now face with increasing urgency. Freshwater seres as a source of drining water, irrigation, and as habitat for aquatic fauna and flora. Nonpoint source nutrients from agriculture, specifically phosphorus, hae caused myriad issues in aquatic ecosystems, including the Eerglades. Eerglades tormwater reatment reas (s are largescale constructed wetlands designed to remoe nutrients from the water column. ngoing research has demonstrated that total phosphorus concentration in water has been reduced from approimately 100 g/ in the influent to 20 g/ in the effluent, which still eceeds the current EP recommendation of 13 g / in the effluent. ccumulation of phosphorus has been obsered in the sediments, specifically Floc and recently accreted sediment ( fractions. he sedimentdeposited total phosphorus concentration declines from the inlet to outlet, creating a phosphorus concentration gradient along the flow in the wetlands. egetation type, submerged aquatic egetation (, and emergent aquatic egetation (E hae also been found to affect phosphorus sequestration. icrobes play fundamental roles in biogeochemical cycles in aquatic ecosystems such as the Eerglades s by mediating transformation of nutrients. hile microbes directly uptae nutrients from the water column, they mae nutrients aailable for plants by decomposing and solubilizing organic matter. arge datasets hae been collected oer the preious years in the s, including nutrient concentrations and ey enironmental parameters (flow rate, water and sediment depth, p, dissoled oygen, and temperature from multiple locations and times. oweer, information about microbial populations in the wetlands that goern nutrient cycles is limited. n this study, we eamined microbial diersity (taa richness and microbial community compositions (CC in the sediment core and the water column along transects (from inlet to outlet of cell 1 (E and cell 3 ( using 1 rN amplicon sequencing. icrobial diersity aried between enironments (water or sediments, egetation types, and across sediment fractions. n general, microbial diersity in water column was lower than that in sediments. he E cell had higher diersity than the cell at each sediment fraction. Floc and sediment fractions had a higher microbial diersity than other fractions in each cell. istance from inlet did not affect microbial diersity ecept in the water column of the cell, in which substantially higher microbial diersity was obsered in inlet samples. Principal coordinate analysis suggests that CC in water column differ from those in sediments. ithin sediment CC, egetation types, sediment fractions and distance from the inlet had effects on CC. Phosphate accumulating organisms (P, taa which potentially play a significant role in P sequestration, were detected in the sediments, and had a distinct spatial distribution pattern across the cells and along the transects. he findings from this study help us to elucidate mechanistic processes that tae place in the wetlands. his study also has implications for water resource management that sees to balance food production and ecosystem conseration.

r. Fujimoto is a esearch ssistant Professor in the oil and ater ciences epartment at the niersity of Florida. e obtained dual Ph.. degree in icrobiology / Ecology, Eolutionary iology & ehaior at ichigan tate niersity. is current research inoles nutrient cycles in Florida’s inland lakes and Everglades wetland systems.

Contact nformation asanori Fujimoto, 15 cCarty all , ainesille, F, 3211

77 12th International Symposium on Biogeochemistry of Wetlands

Kevin A. Grace, oma ek Environmental nc. ockledge F

trient l caracteristics ollowing sediment ressension were investigated or two smerged aatic vegetation secies ara s. a macroalga and aas gadalensis a vasclar macroyte tat are common and native to sallow reswater environments in ot Florida. ese secies occy mc o te otlow region o Everglades tormwater reatment reas s wic are constrcted wetlands wit ltralow target otlow concentrations g or less.

ntact sediment cores were collected rom ara and aas eds in several s as well as rom te nearsore region o ake keecoee. e cores were relooded in te laoratory wit low ntrient water rom te otlow o ell a wellerorming low way. ricial sediments were ressended into te overlying water and tridity osors and ammonia concentrations were reeatedly measred in te water colmn ater and mintes r and days. second ssension event was accomanied y an amendment o into te overlying water tat increased concentrations y g.

itot a amendment sediment ressension reslted in increased water colmn total concentrations and tridity at te minte samling interval comared to te relood water rior to sediment ssension. evels o tese constitents ten decreased ater mintes and remained relatively low in all treatments over te net days. Following te amended ssension event water colmn concentrations also eaked mintes ater sediment ssension ten declined t stayed well aove reamendment vales. o dierence in tridity or water colmn was oserved etween te ara and aas sediments rom eiter ake or sites. owever ammonia concentrations were consistently iger ollowing ressension o sediments rom ara eds relative to aas eds. ring ot amended and namended trials water colmn in all sediment treatments remained iger tan ressension concentrations. y contrast sediments collected rom te otlow region o an witot ell a wellerorming low way dominated y emergent vegetation ya s. and ladim amaicense sowed te lowest water concentrations min. to d ater ssension wit vales eal to or lower tan control cores witot sediment. verall or reslts sowed tat te greatest eects o sediment ssension on water colmn were transient t a small eect o ressension ersisted over time and cold e signiicant in te low environment o otlow regions.

r. race is an ssociate cientist wit Environmental and as over years o eerience in osors cycling in aatic ecosystems treatment wetland otimiation water ality assessments and iomonitoring. e earned a in oil and ater cience rom te niversity o Florida and in ainesville F.

ontact normation evin race Environmental nc. t rive ite ainesville F

78 April 23-26, 2018 | Coral Springs, Florida, USA

Lauren N. Griffiths1,2, illiam i,, an alo ei cool o eosciences niversity o ot Florida ama F Everglades etland esearc ark Florida l oast niversity ales F

trient removal y a . a ran stormwater treatment wetland system in a a waternatre ark in sotwest Florida as een stdied or over two years sggesting tat te wetlands are signiicant sinks o ot osors and nitrogen. e irst two years o water ality stdies ave indicated a sligtly decreasing aility or total osors concentration redction wit average total osors redctions o wereas nitrogen retention as remained consistent at aot redction. is stdy is investigating te imortance o vegetative and sedimentation intrasystem rocesses in aecting ntrient concentrations and les trog tese wetlands. egetation samles are collected every si monts in te dry and wet seasons to estimate rodctivity iomass and ntrient storageretention in te vegetative tisses. dditionally gross sedimentation measrements along wit sediment ntrient analyses every si monts estimate te role o sedimentation on ntrient retention. ngoing water ality researc sggests tat nitrogen and osors loading rates are . g m yr and . g m yr resectively. reliminary reslts sggest tat sedimentation is a larger actor in ntrient retention tan vegetative take. ter one year data sggests gross sedimentation rates o . . mm day and ntrient retention o aroimately . g m yr and . g m yr and. it sedimentation o ntrients iger tan ntrient loading into te system we teorie tat ressension is a maor inlence on te system. ngoing researc ocses on te etent to wic ressension lays a role in te ntrient storage and reintrodction to te system to attemt to determine te eected liesan o te wetlands’ nutrient retention caailities and allow land managers to etter nderstand ow te wetland system is working to otimie ntrient retention.

io aren riits is in te eology .. rogram at te niversity o ot Florida. e is a gradate assistant at te Everglades etland esearc ark in ales Florida wit researc ocsing on water ality and ntrient cycling in created reswater wetlands as well as caron seestration in mangrove systems.

ontact normation aren riits Everglades etland esearc ark aysore r. ales F

79 12th International Symposium on Biogeochemistry of Wetlands

Iikka Haikarainen1,2, nliina kinen,, eei kk,, ika okiakoki, Elia almeenmki,, akk okinen,, nnalea oila an ai ilaie, tion nstitute or nterte toseri n rt ste iene eser niersit o esini esini inn ertent o riutur ienes niersit o esini esini inn innis eteorooi nstitute esini inn

etne is n iortnt reenouse s it inresin toseri iin rtio ore orests re trition onsiere net sin o toseri rini euse o oiiin teri itin erte soi er rees re eonstrte to e e o eittin ro teir stes ossi trnsortin neroi roue ro eeer soi ers urter trees t s ineenent soures o neroi roue itin tree stes or eroi in te tree eroi roesses e inestition o tis reent introue soure o it ree nee o ressessin nes o te ore orests e esure tree ste n orest oor ues on esotroi rine etn orest in e soutern inn urin roin sesons in n ree tree seies on ir ela een or srue iea aie n ots ine in lvei ere seete to e stuie s te reresent te ost oon seies t te ore eettion one etne ues ere esure ro in tot se trees on to ierent ots rti reste ot ere te ines ere reoe to eerient rise te ter te ee n ontro ot treesseiesot e seete tree ires ro te rti reste ot to esure so u rition in te erti roie o te trees o in insit to te nis e rterie te iroi ounities n untiie etnoeni n etnotroi untion enes m n mo resetie in te et n ition e esure te otenti o roution n onsution ro et roie n oss ses

e t te rti reste ot s ener – oser to te soi sure ore to te ontro ot ere te et s on ere t et o orest oor s sin o t ot o te ots tou ute s oer t te rti reste ot e oun s eissions ro te stes t ot ots ie ier n ore oinnt eissions ourre t te rti reste ot etne eissions ro ires in t te rti reste ot ere ier ore to te eission rtes o te se trees in e ste eissions erese eonenti oin u t te ste erti roie iroi eerients soe tt neroi roution oition otenti uner n te ount o etnoens ere ier in te et o te rti reste ot

e ierene in ues o trees eteen te ots suest tt is or reutor o tree ues on orestr rine etn is toeter it te osertion o ier roution otenti in te et t te rti reste site suort te otesis tt tree eitte oriintes ro te soi

i irinen ors s stuent in roet ousin on orest nis is eertise is in ie n ortor esureents o ues in trees n tors etin trnsort n ene roesses

ontt nortion i irinen ertent o riutur ienes tortnonri niersit o esini esini inn

80 April 23-26, 2018 | Coral Springs, Florida, USA

Rachel Joy Harris1, an oel, Kenneth ‘Bud’ Howard, Eliae nne ell,,, elena oloaiele,, an le inon

ts (≥ 71 MPN/100 mL per EPA) in some sections of the Loxahatchee River. From initial (sediments, wrack and water). We collected surface (≤ 2cm) sediment composites along with surfa 3 meters). Our results support previous studies of microbial presence on substrates, suggesting that in Florida’s

BIO: The Loxahatchee River District (LRD)’s mission is to protect and preserve the Loxahatchee River watershed. LRD’s WildPin

81 12th International Symposium on Biogeochemistry of Wetlands

Jordan Heiman, ndo ran, Bo aow, and a oa Koonhare

82 April 23-26, 2018 | Coral Springs, Florida, USA

Janet Ho and a haer

a aronana hau

Janet Ho is a master’s student studying how wetland disturbances alter biogeochemical processes. Janet has worked in freshwat

83 12th International Symposium on Biogeochemistry of Wetlands

RIVER SITE’S A George M. Huddleston III1, hae H aer and nna Kno ynerra orporation reenille aannah ier ational aboratory iken

n ctober of a constructed wetland treatment system began receiing a combination of stormwater and wastewater from the 01 outfall located at the U.S. Department of Energy’s Savannah River Site (SRS) in South arolina. he constructed wetland treatment system was designed to treat approimately one million gallons per day of stormwater from a acre watershed of total flow and effluent from research laboratories of total flow at . he outfall is an permitted discharge and prior to construction of the wetlands contained copper at leels toic to auatic organisms.

he conceptual design of the wetland treatment system was deeloped from pilot mesocosm studies to identify key aspects of wetland function and performance. he pilot studies determined specific design parameters such as physicalchemical characteristics of hydrosoil appropriate hydraulic retention time and selection of wetland egetation effectie for copper attenuation. he fullscale constructed wetland system consisted of an upstream retention basin that proided consistent flow ia graity to eight oneacre wetland cells planted with giant bulrush hoenoetu aornu.

he constructed wetland system has been operational and compliant since . ueous copper concentrations near the wetland outfall aerage approimately g. ediment is the primary sink for copper in the wetland system with copper concentrations greater in the organicrich surface layer. opper is generally more mobile in the floc layer than the inorganic sediment layer. educed mobility in inorganic sediment is associated with higher copper concentrations in residual organic and oide fractions.

he constructed wetland system receied recognition from the .. epartment of nergy and .. nironmental rotection gency egion as a model application of sustainable technology haing saed oer million oer the life of the system.

r. eorge att Huddleston is a enior echnical eader at ynerra orporation a sciences and engineering firm headuartered in Greenville, South Carolina, USA, and an adjunct professor of at Clemson University. Matt’s interests include assessment and rehabilitation of auatic ecosystems ecological risk assessment and constructed treatment wetlands.

ontact nformation att Huddleston ynerra ier treet uite reenille

84 April 23-26, 2018 | Coral Springs, Florida, USA

RRIAE IRA IEEIA RESSES I A ARVE ERAE RIA SAT ARS Haaend tenuer, Nia R. Hurst, ohua Brethaut, and a haer University of Central lorida, rlando, ., USA

urricanes and their associated storm surges, tidal inundation, and rainfall can have a significant impact on coastal marsh systems y delivering allochthonous sediment, nutrients, and seaater to coastal areas. Due to the rapid response of iogeochemical cycling to these disturances, the impact is often difficult to capture and necessitates otaining timely data oth efore and after storm occurrence. Several studies have examined the effect of hurricanes on coastal areas y using data measured months and years prior to a hurricane occurring, ut few have data within only weeks of a storm’s landfall. In the Merritt sland ational ildlife Refuge in revard County, ., UC researchers have een studying the impact of mangrove encroachment on iogeochemical cycling. The consistent monitoring of transects in Merritt Island, located within Hurricane Irma’s cone of influence, provided an excellent opportunity to examine Irma’s impact on nutrient cycling in a Florida salt marsh.

urricane rma made landfall in the state of lorida Septemer 1011, 01. Soil cores and ater samples ere collected along a point vegetative transect, spanning the transition from marsh (th ata) to mangrove (enna ernan) dominated vegetation in the ndian River agoon (southeast lorida). Samples ere collected less than two weeks after Irma’s landfall and compared to data collected less than to ees efore landfall. hysiochemical (extractaleioavailale nutrients, poreater nutrients, p, and salinity) and microial (iomass) properties ere analyed. Soil ioavailale nitrate, phosphorus, and microial iomass generally decreased in all plots after the hurricane, hile soil ammonium, p, and ater salinity increased. oreater phosphorus and ammonium increased in the upper 1cm of soil in the th and transitional plots (000), ut decreased in the mangrove plots (0100). Results suggest that hurricanes have the potential to significantly alter soil and poreater iogeochemistry immediately after occurrence y increasing availale nutrients, particularly ammonium, and decreasing microial iomass, liely due to salinity stress. hese results also suggest that hurricane impacts differ ased on vegetation as trends ere not alays similar eteen the marsh and mangrove plots. Assessing the impact of hurricanes on iogeochemical cycling in etlands can help to understand ho storms affect shortterm nutrient cycling and the potential for nutrient release.

ia urst is a a Manager and Research Assistant in the Auatic iogeochemistry a at the University of Central lorida. Nia’s research focuses on etland iogeochemical responses to restoration ith the emphasis of assessing and improving etland aility to mitigate auatic pollution.

Contact nformation ia urst, University of Central lorida, 110 ira Dr., rlando, ., USA 1

85 12th International Symposium on Biogeochemistry of Wetlands

REI ITRE REVA ERTAIT R ERATI ISSISSII RIVER SEIET IVERSIS ITRATE RETI RATES I TRET ITIS Nia R. Hurst1, ohn hte, Kehu u, and rano en niversity of entral Florida, rlando, F., ouisiana tate niversity, aton ouge, ., niversity of New Hampshire, urham, NH.,

ediment diversions along the lower Mississippi iver have een proposed to help slow the rate of wetland loss in coastal ouisiana y transferring river sediment to coastal marshes, aiding in wetland accretion. ediment diversions can deliver a sediment susidy to eroding ouisiana coastal wetlands, ut they will also deliver significant amounts of nutrients primarily N as N to receiving asins with potential for environmental impact. There is a paucity of data on nitrate reduction rates in the “nearfield” area of diversions, where turbulent conditions impart significant shear stress on the sediment surface, suspending fine grained sediments. It is critical to determine nitrate reduction rates in this nearfield environment to understand the total impact of N on diversion receiving asins and to reduce the uncertainty of nutrients delivered y river sediment diversions. Triplicate sediment cores were collected from three sites within a mudflat in an active deltaic setting in coastal ouisiana and suected to shear stress using a flowthrough erosional microcosm system for hrs. Nitrate reduction rates were determined under high, medium, and ero shear stress conditions of ., ., and a, respectively. Nitrate reduction rates under these conditions were ., ., and . . mg N m day, respectively, with rates increasing as shear stress application increased. These results indicate that turulent flow conditions, indicative of large river diversions, can significantly increase nitrate reduction rates, even in a welloxygenated water column. esults from this research can help inform diversion models and assist natural resource managers in predicting potential nutrient impacts of river diversions on coastal receiving asins.

I Nia Hurst is a a Manager and esearch ssistant in the uatic iogeochemistry a at the niversity of entral Florida. Nia’s research focuses on wetland iogeochemical responses to restoration with the emphasis of assessing and improving wetland aility to mitigate auatic N pollution.

ontact Information Nia Hurst, niversity of entral Florida, ira r., rlando, F.,

86 April 23-26, 2018 | Coral Springs, Florida, USA

AIS ETAS TRIETS SEIETS A AR I EITERRAEA STRTE ETAS REEIVI ARITRA R Carles Ibáñez, uno aoa, er urda, tora oae and ert ora T, nstitute for rifood esearch Technoloy, uatic cosystes rora, atalonia, pain

arsh restoration is as an effective tool to reove nutrients and etals via plant uptae, icrobial activity and soil accuulation owever, few studies have attepted to uantify both nutrient and etal accuulation and reoval in editerranean restored arshes, includin also accretion rates and carbon seuestration This research ais to assess chanes in water and soil etals, nutrients, sedients and carbon in oliohalinerestored arshes set in abandoned rice fields, includin a first yr study in sall eperiental wetlands and a second yr study in a larescale constructed wetland

n the eperiental wetlands two freshwater type treatents were tested river irriation water and rice field drainae water , as well as three different water levels ependent variables as a function of water type and water level were tested by a partlynested eperiental desin usin eperiental units each ifferences in water level did not cause sinificant differences in etal reoval and accuulation in soil arshes in both water types owever, results showed that sinificantly hiher n, b and n input fro caused hiher ean of reoval iher u concentration fro also caused hiher u reduction ean values of o, r, u, Ni, b, and n in soil were hiher in the treatent characteried by hiher plant bioass, whereas ean accuulation rates of s, a, r, u, Ni, b, and n were hiher in the treatent because of hiher accretion rates iher nutrient dischare fro rice field runoff caused sinificantly hiher seasonal N and reduction and respectively There was a seasonal eport in T and and , respectively in arshes receivin river irriation water inificantly lower soil redo and hiher total aiu aboveround bioass in arshes receivin river irriation water were associated with lower N reduction and hiher i reduction and respectively iher sedient dischare fro rice field runoff was associated with hiher seuestration rates yr copared with arshes receivin river irriation water yr iher water levels also increased sinificantly and i reoval and seuestration rates within both water type treatents

n the larescale constructed wetland ha two different turnover rates and water levels are bein tested The onoin study ends in , so we present preliinary results of the first yr , onthly saplin reardin reoval of etals and nutrients in water ean etal reoval rates were positive for l , e , n and r and neative for and s ean nutrient reoval rates were all positive N , N , N , i and owever, , N and i showed net eport in soe periods

esults suest that wetland plants liely favored soil etal adsorption throuh soil oyenation and hihliht the utility of restored arshes as pollution filters in coastal wetlands with sinificant soil accretion This study indicates that editerranean oliohaline restored arshes are very efficient reovin N and soe etals l, e, n, u usin both river irriation and rice field drainae waters and also provide seuestration and sedient accretion in a contet of relative sea level rise

r be is enior esearcher at the uatic cosystes rora of T and forer ead in the period e has years of research eperience in the field of auatic and wetland ecoloy, water resources and coastal anaeent, with ore than papers published in peerreviewed ournals

ontact nforation arles be, uatic cosystes rora, T, ant arles de la pita, atalonia, pain

87 12th International Symposium on Biogeochemistry of Wetlands

STIIETRI TRS IRIA EE ATIVITIES A TRIET I I ETAS Kanika S. Inglett niversity of lorida, ainesville, ,

Nutrient availability and cyclin in wetlands is dependent on activities of etracellular enyes that for the bottle nec in oranic atter decoposition icrobes epress enyes in response to their eleental deand, therefore bioass nutrient and enye stoichioetry is believed to be relatively stable as icrobes follow the principle of hoeostasis owever, in anaerobic wetlands, carbon processin efficiency and nutrient reuireents are ore diverse than aerobic systes leadin to altered stoichioetries and enye ratios ew studies have eained these relationships in wetlands with the oal of predictin ineraliation in a variety of carbon and nutrient conditions n this study we investiate the effect of veetation and eternal nutrients on icrobial bioass and enyatic stoichioetry in two contrastin wetlands systes one doinated by subered or eerent auatic veetation, and both receivin nutrient runoff fro the adoinin ariculture icrobial bioass and enye ratios were fleible and varied in the two systes and did not follow predictable patterns fro nutrient stoichioetry Typical patterns were observed for icrobial bioass abundances and relative enye activities as oranic coponents proressed fro litter to floc, and soil ifferences were observed between the two veetation types with hiher overall nutrient ratios and ore nutrient liitation in the esults confir the eneral hypothesis of increased liitation in and reater overall icrobial activity in The differences between the two veetation types is larely based on abundances of available in bioass structural carbohydrates and potentially secondary cheical reactions e, with a leadin to difference in icrobial counities plications of these findins with respect to other wetlands systes and lobal storae odels will be discussed

r nlett is a es ssistant rofessor in the oil and ater ciences epartent at the niversity of lorida er research focuses on the echanistic understandin of icrobial processes and icrobial ecoloy to predict their responses to environental chane

ontact nforation ania hara nlett, nvironental icrobioloy, oil and ater ciences epartent, carty , niversity of lorida, ainesville, ,

88 April 23-26, 2018 | Coral Springs, Florida, USA

RI EETS IRIA STIIETR A EE ATIVIT I TE EVERAES STRATER TREATET ETAS ara Baer, Patrick W. Inglett1, Kana nett, Kn niversity of lorida, ainesville, , outh lorida ater anaeent istrict, est al each,

n constructed wetlands lie the torwater Treatent reas Ts, icrobial processes play a ey role in nutrient reoval pecifically, etracellular enye production reulates oranic decoposition, nutrient ineraliation, and indicate overall nutrient liitation ydroloic flow and its anaeent directly effects nutrient distribution and loadin, which liely alters icrobial nutrient deand and functions such as enyatic activity however, there are few studies that asses the effect of flow on icrobial stoichioetry and enye activities n this study, icrobial bioass , N, and and etracellular enyes for phosphoonoesterase, APA and phosphodiesterase, BisP), C (βglucosidase, BG), and N (Leucine aminopeptidase, LAP and βN acetyllucosainidase, N were analyed at inflow, idflow, and outflow stations of T ell durin stanant, low, oderate, and hih flow conditions n eneral, enye activity increased fro inflow to outflow, with larest increases observed in related enyes nder flowin conditions, overall enye activity was stiulated and accopanied by hiher icrobial bioass, especially at the outflow ncreased icrobial bioass and related enye activity indicates an effect of flow on icrobial abundance and a potential role of flow in liitation at the outflow oparin these results of icrobial enyes with water uality patterns and decoposition ay better predict the role of icrobial activity on nutrient liitation under stanant and flow conditions

r nlett is an associate professor of wetland bioeocheistry in the oil and ater ciences epartent at the niversity of lorida e has ore than years of eperience worin with nutrient and ecoloical relationships in laes, sprins, salt arshes and wetlands

ontact nforation atric nlett, epartent of oil and ater cience, niversity of lorida, carty all, ainesville, ,

89 12th International Symposium on Biogeochemistry of Wetlands

ARI RATE RIVES IRIA TRIET IITATI A EE ERESSI Patrick W. Inglett1, ean h, and Kana hara nett niversity of lorida, ainesville, , niversity of aryland, rostbur, ,

ecent developents of enyebased decoposition odels hihliht the iportance of enye inetics with warin, but ost odelin eercises are based on studies with a stepwise warin This approach ay as the effect of teperature in controllin insitu activities as in ost ecosystes soil teperature chane ore radually than air teperature e conducted an eperient to test the effects of contrastin warin rates on the inetics of , N, and deradation enyes in subtropical peat soils e also wanted to evaluate if the stoichioetry of enye inetics shifts under contrastin warin rates and if so, how does it relate to the stoichioetry in icrobial bioass ontrastin warin rates altered icrobial bioass stoichioetry leadin to differin patterns of enye epression and icrobial nutrient liitation ctivity hiher a and efficiency lower of acuisition enyes were reater in the step treatent however, epressions of nutrient N and acuirin enyes were enhanced in the rap treatent at the end of the eperient n the step treatent, there was a typical pattern of an initial pea in the a and drop in the for all enye roups followed by later adustents n the other hand, a consistent increase in a and decline in of all enye roups were observed in the slow warin treatent These chanes were sufficient to alter icrobial identity as indicated by enye and bioass stoichioetry with two apparently stable endpoints under contrastin warin rates This observation resebles the concept of alternate stable states and hihlihts a need for iproved representation of warin in odels

r nlett is an associate professor of wetland bioeocheistry in the oil and ater ciences epartent at the niversity of lorida e has ore than years of eperience worin with nutrient and ecoloical relationships in laes, sprins, salt arshes and wetlands

ontact nforation atric nlett, epartent of oil and ater cience, niversity of lorida, carty all, ainesville, ,

90 April 23-26, 2018 | Coral Springs, Florida, USA

TERA ATTERS I EE ATIVIT A ATERIA IT STRTRE TE SERE TE ETA ARTE TYPHA LATIFOLIA Bra tone, Colin R. Jackson niversity of ississippi, niversity, ,

n iportant but often overlooed aspect in wetland bioeocheical cyclin is the decoposition of standin aerial dead plant atter ritical to the decoposition of this aterial is the establishent of a icrobial decoposer counity on the leaf surface, or phyllosphere, and the concoitant release of etracellular enyes associated with carbon, nitroen, and phosphorus ineraliation

e deterined bacterial diversity and counity structure on the leaf surface of ha atoa broadleaf cattail over the course of a year, and assayed the activity of lucosidase, Nacetyllucosainidase, and phosphatase, enyes involved in the cyclin of carbon, nitroen, and phosphorus, respectively aples were taen bionthly and also before and after rain events that could stiulate bioeocheical processes in the phyllosphere

e found a stron seasonal pattern in etracellular enye activity with low activity in the sprin and suer on livin leaves and hiher activity soon after leaves bean to senesce ver saller tie scales days, precipitation and solar radiation were the ost consistent drivers of icrobial etracellular enye activity hile hiher enye activity occurred on day of rain events, continuous rainfall across day periods was associated with lowered enye activity n contrast, enye activity was hiher across dry periods with hih direct and diffuse radiation

acterial diversity in the phyllosphere declined followin leaf senescence, while etracellular enye activity increased, reflectin a shift fro predoinantly bacterial counities on the livin acrophyte surface to predoinantly funal counities on standin dead acrophytes hile the decoposition of standin dead T latifolia is liely driven by funal counities, cliatic factors such as rainfall and solar radiation influence both the structure and bioeocheical function of bacterial asseblaes in the phyllosphere of livin wetland acrophytes

r acson is a icrobial ecoloist with years of eperience eainin the structure and function of wetland icrobial asseblaes e has conducted proects in wetlands ranin fro the ulf oast to tropical peatlands, with current research includin assays of enye activity and neteneration seuencin of wetland icrobial counities

ontact nforation olin acson, epartent of ioloy, The niversity of ississippi, niversity, ,

91 12th International Symposium on Biogeochemistry of Wetlands

AT TRS IRIA EE ATIVIT I ETAS Colin R. Jackson niversity of ississippi, niversity, ,

ioeocheical cyclin and oranic atter decoposition in wetlands is driven by the activity of icrobial enyes espite decades of research on icrobial enye activity in wetlands we still have a poor understandin of how environental factors influence the activity of these enyes edient enye activity has been related to paraeters such as wetland veetation type, salinity, sedient particle sie, and sedient depth, as well as to icrobial bioass and cell nubers owever, these relationships are often syste or habitatspecific, ain eneraliations difficult

n studies of saltarshes alon the ulf oast, site appears to have a reater ipact on sedient enye activity than veetation type, suestin that broadscale patterns ay be as, or ore, iportant than finerscale spatial variation Thus, sedient enye activity ay be ecosystespecific and patterns of enye activity seen in one wetland ay not be applicable to siilar systes, even within the sae eoraphic re

hile enyes in wetland sedients have received ost attention, other icroenvironents, such as the surface phyllosphere of wetland acrophytes can show appreciable enye activity This phyllosphere enye activity shows seasonal and teporal variation, with pulses of enye activity bein triered by precipitation events and a shift fro livin plants to those underoin senescence and decoposition uch chanes liely correspond to shifts fro a bacteriadoinated icrobial counity on livin veetation to a funaldoinated counity on decayin acrophytes

espite the advent of net eneration seuencin technoloies that now allow indepth deterination of wetland icrobial counity coposition, lins between icrobial counity structure and enye activity are often unclear n onoin challene is to connect the enoic coposition of wetland icrobial counities to the “phenome”, or functional manifestation of the communities’ genetic capability. Assays of enzyme activity provide insiht into this phenoe and how it connects to wetland bioeocheistry

r acson is a icrobial ecoloist with years of eperience eainin the structure and function of wetland icrobial asseblaes e has conducted proects in wetlands ranin fro the ulf oast to tropical peatlands, with current research includin assays of enye activity and neteneration seuencin of wetland icrobial counities

ontact nforation olin acson, epartent of ioloy, The niversity of ississippi, niversity, ,

92 April 23-26, 2018 | Coral Springs, Florida, USA

EETS R TIE A I ETRATI SRS ISARE R A ERITASE STRATER TREATET AREA R. Thomas James outh lorida ater anagement istrict, est alm each, , A

he tormater reatment Areas As are critical component of the verglades estoration. he eriphyton based tormater reatment Area A as constructed at the donstream end of A as a pilot demonstration of the A technology to achieve ultralo level total phosphorus at the outflo. he acre A cell as scraped to remove muc don to the limestone bedroc. ince it began operation in the A cell has achieved an average annual flo eighted mean concentration of g , the current regulatory longterm limits for the A discharge. An etensive study as conducted to determine the ey factors that contribute to the PSTA cell’s performance. Part of the investigation is the use of Remote Phosphorus Analyzers A at inflo and outflo sites, hich alloed freuent measurements of , i.e. every three hours for . years. This analysis evaluates factors that may contribute to the PSTA Cell’s ability to produce very low TP concentrations at the A cell discharge. he factors evaluated include flo, to different ater level operation periods, inflo concentration, season, and timeofday. reuent measurements using the As should capture short time related events that may not be captured in daily or eely sampling.

ater levels in the cell ere maintained at . feet beteen arch and April and . feet beteen April and eptember . utflo concentration as less during the .foot as compared to the . foot operation. oever, the result as complicated by higher inflo concentrations during the .foot operation period, hich occurred due to replanting activities in the upstream areas of A . he A results indicate that the outflo concentration as not influenced by the time of day.

o compare inflo and outflo concentrations, eely floeighted average concentrations ere used. his time period as selected because the average hydraulic residence time in the A cell is approimately one ee.

ignificant relationships ere observed beteen the inflo and outflo concentrations. An analysis of covariance shoed no significant difference beteen the to operation periods. he nearly identical relationships suggested that ater level differences did not directly influence removal. ptimal flo conditions, here the maimum number of samples less than g occurred, as beteen and cfs for the to operational periods. his result suggested an additional proect, hich as recently completed, to determine if these flo regimes can maintain lo concentrations for etended periods.

r. ames is a rincipal cientist ith over years of eperience ith lae and etland research. e has etensive eperience ith ae eechobee. e recently oined the ater uality reatment echnologies ection as the coordinator for implementing the estoration trategies cience lan for the verglades tormater reatment Areas.

ontact nformation . homas ames, outh lorida ater anagement istrict, un lub oad, est alm each , A

93 12th International Symposium on Biogeochemistry of Wetlands

R EAR T A SI ERR STAE ISTES T ERSTA ERR I A SRES Sarah E. Janssen, an ea, ad Kraenhot .S. eological Survey iddleton SA

ver the past decade measuring the natural abundance of mercury g stable isotopes has become the forefront in methodology for source and process tracing of both bioavailable methylmercury eg and the precursor inorganic divalent g. This approach has gained traction primarily due to the fractionation patterns g undergoes naturally in the environment and allows us to assign fractionation signatures to different chemical and micro biotic transformations. everaging the composite signal of these transformations provides us with an isotopic “fingerprint”. These “fingerprint” compositions allow for the environmental application of Hg stable isotopes for purposes such as the source tracing and even eamining the impact of dietary shifts on g accumulation such as those evident with invasive species. The information gained from mercury stable isotopes improves the understanding of g cycling and provides a diagnostic tool for resource managers interested in the mitigation of g contamination. This presentation will focus on the wideranging applications of stable g isotope research including mechanistic approaches as well as largescale environmental surveys.

The strength of stable g isotope measurements comes from the understanding that different sources of mercury often ehibit uniue signatures due to chemical physical and biological processes that impact them. ne ey process in the g cycle responsible for the manipulation of a source signature is microbial methylation. Results from lab scale studies show that different methylating microorganisms e.g. sulfate and iron reducing bacteria fractionate g to the same etent but can selectively methylate different bioavailable pools of inorganic g. These microscale differences can lead to a wide range of isotope compositions observed in eg in sediments (e.g., δ202Hg = 0.4 to +0.4 ‰). Utilizing these “fingerprints” produced by both the methylation reaction and the original isotopic composition of inorganic g entering the system we can develop source profiles to trac eg transport into the food web. n a regional study of the Florida Everglades, composite signatures of δ202Hg have established three isotopically distinct g sources in fish tissue coastal marsh and terrestriallyderived g. panding the verglades systems to a tertiary tracer approach we can simultaneously trace the impact of photochemical processes on source MeHg (using Δ199Hg) as well as independently trace one component of the source portfolio, atmospherically derived Hg (using Δ200Hg). These differences in isotopic composition can also be used to further eplore variation in g sources in different fish species such as lower photochemical signals (Δ199Hg) in jewelfish compared to mosquitofish, which indicate different food sources. Lastly, the further establishment of isotopic signatures on a regional scale such as the verglades can be used to epand upon national and global isotope studies that define the role of largescale g sources e.g. precipitation dry deposition on bioavailability and eg accumulation in auatic systems.

r. anssen is a post doc at the .S. eological Survey in isconsin. er research focuses on microbial methylation of mercury and the application of g isotopes to understand g accumulation in the food web.

Contact nformation S.. anssen isconsin ater Science Center Research ay iddleton SA

94 April 23-26, 2018 | Coral Springs, Florida, USA

SRS I TE EVERAES STRATER TREATET AREAS Mike Jerauld1, ohn uton, o eBu, Kn nvironmental nc. oclege oth loria ater anagement istrict est alm each

emoval of phosphors from rnoff b the tormwater Treatment reas Ts protects the historicall oligotrophic verglaes from anthropogenic etrophication. The otflow concentrations in the Ts contine to improve with total phosphors T concentrations reaching below g in some of the Ts. ore efforts are neee to achieve the stringent reglator T limits of g longterm an g annal. The concerte estoration trategies cience lan sees to fill gaps in the nerstaning of T internal processes to facilitate the achievement of target ischarge concentrations. ne ongoing component of the science plan is the ientification an antification of seimenttowater colmn fles in the Ts particlarl in the bacen regions where fles col have the greatest inflence on otflow concentrations.

T ell is a wellperforming wellstie T flowwa ominate b sbmerge aatic vegetation. sing in sit batch chambers an porewater eilibrators we measre the net internal loa an the porewater iffsive fl respectivel into the water colmn at inflow mi an otflow regions of ell on for occasions. The internal loaing rate isplae an internal graient from abot g mr at inflow to abot . g mr at otflow comparable to the magnite of the loa avecte b water flows. iffsive fles were low at inflow p to . g mr ecreasing to at otflow therefore iffsion was essentiall negligible as a fraction of the total internal load. These “blackbox” internal load estimates provide a useful context for evaluating potential fl mechanisms an alrea show promise for refining moels of T performance. rrent an ftre research to ientif an antif the inivial biogeochemical mechanisms that contribte to the internal loa ma illminate management strategies to frther rece T otflow concentrations.

BIO: Mr. Jerauld’s research concerns the biogeochemical processes affecting water quality in treatment an natral wetlans. He has wore in wetlans aron the state of loria an has been involve with verglaes stormwater treatment area research for nine ears.

ontact nformation ie eral nvironmental nc. s Hipp lv oclege

95 12th International Symposium on Biogeochemistry of Wetlands

EETS AI RESTRATI I ARITRA ATERSES SRS AIS Alex Johnson1,ara an,ar a urdue niversity est afayette I oil rosion ab est afayette I

xcess phosphorus runoff from agricultural watersheds is a maor water quality concern for many aquatic ecosystems in the Midwestern . One management strategy that has been proposed to buffer high phosphorus loads in impacted watersheds is floodplain restoration in headwater streams often referred to as a twostage ditch. hen inundated floodplains can retain phosphorus through a variety of mechanisms namely physical adsorption biological uptake and sediment deposition during flooding events. It is not known whether there is a fundament change in these mechanisms when a twostage ditch is constructed and develops over time.

To assess changes in phosphorus retention upon creation of inset floodplains we evaluated chemical and physical sediment properties in twostage ditches and nearby unmodified streams in central and northern Indiana during the summer of . oil cores were collected to a depth of cm in the floodplain benches and streambed sediments and water chemistry was evaluated at both the upstream and downstream ends of each reach. ores were homogenied and evaluated for waterextractable equilibrium phosphorus concentrations at ero net sorption sorption index I oxalateextractable percent organic matter and particle sie distribution. In order to assess removal potential during storm flow a separate paired experiment was carried out where intact soil cores were collected to a depth of cm seasonally from three of these streams during the same summer. These cores were incubated in a flowthrough system using stream water and fluxes were calculated using the difference in inlet and outlet dissolved reactive phosphorus concentrations.

esults of these experiments show no significant difference in any soil physical or chemical properties between the twostage ditch and its control counterpart for either floodplain or stream sediments. dditionally phosphorus fluxes from intact cores showed no significant differences between the twostage and control reaches. owever phosphorus sorption index an indication of phosphorus sorption capacity was significantly correlated with organic matter suggesting that sorption to organic matter directly and indirectly through humic el complexes can significantly affect sorption dynamics. urthermore organic matter content was largely driven by storm events with antecedent dry days being the most significant predictor of organic matter on both floodplain and streambed sediments. This could indicate that sorption to biofilms or other more temporary pools of organic matter in the soil are being flushed periodically. These results challenge the assumption that soil phosphorus sorption parameters are static in time and suggest that when floodplains are inundated soil properties will strongly influence dynamics. This is particularly important in agricultural landscapes where past management practices affect both quality and quantity of organic matter and .

Bio: Alex Johnson is a second year master’s student at Purdue niversity. is research focuses on coupled biotic and abiotic system in a variety of ecological engineering management strategies with a particular focus on phosphorus cycling.

ontact Information: lex Johnson Mcmillan esearch roup niversity treet est afayette I

96 April 23-26, 2018 | Coral Springs, Florida, USA

E TESE TIS IS T IE TE TER EVAATI ETA TRIET STIIETR A ESTASIS I A STRIA TREATET ETA Paul Julian II1, an rht, tean erer, ueh K Bhoa, Kn, d aando, odd orne niersity o lorida oil and ater ciences eartment t Pierce A niersity o lorida oil and ater ciences eartment ainesille A outh lorida ater anaement istrict ater uality reatment echnoloies est Palm Beach A

coloical nutrient stoichiometry is the result o chanes to elemental mass alances due to ioloical and ecoloical rocesses aluatin the relatie chanes in caron nitroen and hoshorus P ratios in auatic and terrestrial ecosystems can adance our understandin o ioloical rocesses nutrient cyclin and decomosition o oranic matter utrient stoichiometric homeostasis relates amient stoichiometric conditions to a secies stoichiometric comosition Prior studies suest that lant secies deree o homeostasis ranes rom ea to nonhomeostatic he deree o homeostasis o a lant secies could indicates its sin strenth n etland ecosystems eetation is a sieale hihly ariale and dynamic sin o nutrients

his study inestiated etland nutrient stoichiometry across seeral ecosystem comartments ie ater soil eetation in to treatment cells o the erlades tormater reatment Area south lorida A hese cells include an emerent auatic eetation system dominated y ha cattail and a sumered auatic eetation system comosed o secies such as hara s musrass and otaeton s ondeed his study demonstrated that and P stoichiometry can e hihly ariale ithin and eteen etland ecosystem comartments in a Plimited ecosystem enerally total P declined alon the lo ath in all ecosystem comartments hereas trends in total and ere not consistent eanhile :P relationshis increased as : and :P aried in arious comartments alon the treatment lo aths utrient homeostasis o dominant emerent and sumered auatic eetation A and A resectiely ithin to treatment cells as also inestiated his study conirmed the hyothesis that etland eetation is nonhomeostatic alon dierent eetation communities ith homeostasis coeicients o and or A and A resectiely

Assessment o etland nutrient stoichiometry eteen and ithin ecosystem comartments suest decoulin o oranic matter decomosition rom nutrient mineraliation hich may hae siniicant inluences on nutrient remoal rates and contrastin dominant eetation communities urthermore eetation ithin the As roides a lare nutrient sin ith relatiely constant utae ressure suestin that eetation alon ith other actors inluence amient nutrient conditions ithin a ien treatment cell

B: r Julian is a Ph student ith the niersity o lorida oil and ater ciences eartment in the etland Bioeochemistry aoratory studyin etland ioeochemical rocesses seciic to the erlades ecosystem and stormater treatment areas etland nutrient siralin stoichiometry and auatic roductiity

ontact normation: Paul Julian etland Bioeochemistry aoratory oil and ater ciences et niersity o lorida ort Pierce

97 12th International Symposium on Biogeochemistry of Wetlands

RIVER RS TR IT EVAATI RATER A SRAE ATER ETIVIT A ITS IIATI RIARIA IEEISTRA E Paul Julian II1, re ether, an rht niersity o lorida oil and ater ciences eartment t Pierce A immera atchment anaement Authority orsham ic A

Alteration to the hydroloic alance o a rier ecosystem can hae roound eects on its ioeochemistry and suseuent ecoloy and use conersion chanes in ater demand or aricultural and domestic uses and alteration o natie eetation are contriutin actors to stream and rier hydroloy and can resultin in chanes to reional roundater eleations his altered hydroloy can result in surace ater saliniation throuh the transort o saline roundater accumulation o salts in soils assie runo and direct dischare o saline roundater to surace aters tratiied ools o saline ater can occur due to reduced surace ater lo in comination ith roundater dischare o saline ater resultin in neatie ecoloical imacts aliniation o reshater streams and riers hae een osered across the loe hoeer most notaly saltaect riers and streams occur throuhout lare areas o southeastern Australia esecially in the loer lains in northern and estern ictoria Australia

his study ealuated the chane o ater manaement and reional hydroloy relatie to surace ater saliniation and its eect on iota and ioeochemistry ithin the loer immera ier he loer immera ier has a hih enironmental alue ith lare stretches o intact riarian and instream eetation and many threatened auna secies o concern he rainallruno relationshi alon dierent reaches o the loer immera ier hae chaned oer time due to chanes in climate and lo reulation y dams and eirs hanes in ater manaement throuh the roision o enironmental los has siniicantly reduced the extent and duration o hih salinity eents at ey locations alon the loer reaches o the rier urthermore concurrent ith reduced salinity eents nutrient concentrations ithin the rier hae eun to decline n addition to imroe ater uality ish and macroinerterate communities hae reounded

on term ecoloical monitorin has demonstrated that lo and ater uality issues are critical actors that inluence the oerall ecoloical interity o the immera ier hile some arue that enironmental los in loland riers are insuicient to adeuately reduce harmul ecoloical imacts enironmental lo manaement is the central tenant to restorin ater uality and ecoloical health in the ier ince the creation o ater savings to be provided as environmental flows in the early 2000’s environmental conditions have improved. ithin a decade the ier has imroed rom a hihly imacted ecosystem to a hihly alued ecosystem and enironmental asset ithin a decade as a direct result o the imlementation o enironmental lo restoration

B: r Julian is a Ph student ith the niersity o lorida oil and ater ciences eartment in the etland Bioeochemistry aoratory studyin auatic ioeochemical rocesses etland nutrient siralin stoichiometry and auatic roductiity

ontact normation: Paul Julian etland Bioeochemistry aoratory oil and ater ciences et niersity o lorida ort Pierce

98 April 23-26, 2018 | Coral Springs, Florida, USA

TRS E IASE A EAI R A EATA Hojeong Kang, hr reean and unhun K onsei niversity eol orea 2angor niversity angor mithsonian nvironmental esearch enter dgewater

ncreases in dissolved organic carbon concentrations in streams have been recorded at locations all over the world inclding rope orth merica and ast sia over the past few decades. large proportion of originates from watersheds with organic soils sch as peatlands which store over a third of global soil organic carbon. recent report based on a field maniplation eperiment sggested that changes in p cased by a decline in acid deposition may be a ey driver for the large release of from peatlanddominated watersheds. owever the stdy failed to reveal a biological linage between p and releases even thogh microbial processes may be responsible for sch changes in addition to chemical reactions and physical leaching from the peat matri nder elevated p conditions. he present stdy aimed to investigate biogeochemical linages between p increase and prodction in peatland by analying chemical properties enyme activities and microbial commnity strctre in global peatland.

e collected soil and water samples from peatland in orea apan and ndonesia. henol oidase and hydrolases activities were measred by sing artificial model sbstrates. acterial commnity strctres were assessed by seencing and t and the abndances of microbes and fnctional genes were estimated by real time . n addition to the field srvey two p maniplation eperiments were condcted both in the field and lab conditions. inally we either nocot phenol oidase with an inhibitor or added phenol oidase in peat slrry samples to reveal the role of phenol oidase in leaching from peat matri.

he p ehibited a positive correlation with phenol oidase across the sites. hen all data were plotted in a single graph phenol oidase activity appeared to be eponentially associated with p. owever neither temperatre nor water level ehibited significant correlations with phenol oidase indicating that p is the main controlling variable for phenol oidase. he maniplation eperiment showed the same trend where phenol oidase activity was significantly associated with p changes. n addition phenol oidase activity was positively correlated with phenolics in porewater in each site indicating that phenol oidase activity indeed is associated with the release of phenolic material from peat soils. ddition of phenol oidase sbstantially increased phenolic concentration in peat slrry while the inhibition of the enyme decreased phenolics in the sample. t was also fond that p effect on phenol oidase is both direct and indirect throgh changes in microbial commnity as well as abndances. n smmary or reslts showed that p increases in peatland by redced atmospheric deposition activate phenol oidase in peat which then accelerates leaching to adacent aatic ecosystems.

r. ang is rofessor at chool of ivil and nvironmental ngineering onsei niversity in orea. e has pblished over 00 international ornal papers focsing on of wetland ecosystems. e is crrently serving as an ssociate ditor for ‘Ecological Engineering’ and editorial board members for ‘Microbial Ecology’, ‘’ and ‘Ecosystem Services’.

ontact nformation oeong ang chool of ivil and nvironmental ngineering onsei niversity onseio 0 eol 022 orea

99 12th International Symposium on Biogeochemistry of Wetlands

EETS RRIAE IRA ASTA EVERAES ATERS I RIA A Christopher Kavanagh, ah ratto, ten ational Par Service, Everglades ational Par, lorida ay nteragency Science enter, , S

ater ality along northern coastal lorida ay is mediated by the everglades etland processes and ater volme and residence times in the atershed. ategory hrricane rma in September prodced a large fl of srface ater and marsh ntrients affecting ater conditions in coastal aters of Soth lorida. Monitoring efforts targeted the dissolved ntrients and phytoplanton response to the ind distrbance and increased fl from everglades marsh and mangrove coastline. Physical, chemical and biological parameters ere traced in lorida ay aters in the aftermath of the hrricane inclding dissolved oygen, nitrogen, phosphoros, organic carbon, and chlorophylla. eslts of the magnitde and dynamics of ater conditions in the months folloing the storm event are described.

Mr. hristopher avanagh is a marine ecologist ith more than years of eperience in coastal and estarine research and management. e has eperience ith ater ality and coastal processes, and is crrently engaged in proects dedicated to restoration of the Everglades and lorida ay.

ontact nformation hristopher avanagh, Everglades ational Par, lorida ay nteragency Science enter, verseas ighay, ey argo, , S

100 April 23-26, 2018 | Coral Springs, Florida, USA

AT IS TE AR SEESTRATI TETIAL OF AUSTRALIA’S COASTA AI RESTS Jeffrey Kelleway1, e antan, Ken Krau, ar errano, ara ernanda dae, onnor orha and au aer Macarie niversity, Sydney, S, stralia ational etland esearch enter, afayette, , S School of Science, entre for Marine Ecosystems esearch, Edith oan niversity, oondalp, , stralia stralian ivers nstitte, riffith niversity, athan, , stralia

oastal floodplain forests are ecological commnities on aterlogged or periodically inndated flats, drainage lines, lae margins and estarine fringes associated ith coastal floodplains, composed of auarna and eaeua species. n estarine settings, they may be inndated dring large spring tides, introdcing slphates and salinity to the soils. he contemporary biogeochemistry of coastal floodplain forests may also be inflenced by sealevel history. or eample, a sea level highstand in the early to midHolocene along Australia’s coastlines has reslted in elevated slphate concentrations and potential acid slphate soils in coastal floodplains. ogether, these contemporary and historical factors may generate biogeochemical conditions similar to those of presently defined ‘blue carbon’ ecosystems (mangrove, tidal marsh, seagrass) – that is, aterlogged, slphaterich soils here decomposition of organic matter is sloed and methanogenesis is sppressed.

e present first data from mltiple stralian coastlines on carbon stocs above and belogrond, soil carbon accmlation rates based on radiometric Pb dating and decomposition rates shortterm litterbag eperiment in coastal floodplain forests. e compare or findings ith data from more comprehensively stdied mangrove, tidal marsh and seagrass ecosystems. r antifications of the carbon and srface accmlation dynamics of coastal floodplain forests have implications for carbon acconting and mitigation in stralia, ftre management of coastal floodplain forests, and global directions of ble carbon research and policy.

r. elleay is a Postdoctoral esearch ello ith over years of eperience condcting etland research and management oriented proects. is research focsses on the ecosystem processes associated ith etland change and the science and implementation needs of ble carbon proects.

ontact nformation effrey elleay, epartment of Environmental Science, , Macarie niversity, S stralia

101 12th International Symposium on Biogeochemistry of Wetlands

URA STORATR TLAS AS OL IOOCICAL SSTS LAT SALT A SULFAT Lauren Kinsman-Costello1, nne eeron, edro eaneda, Hae Buena, aura uano and aor hae ent tate niversity, ent H, A ndiana niversity, , A

vidence is emerging that freshater ecosystems embedded in an urban landscae are distinct from those associated ith rural land use and land covers ecifically, urban auatic systems bear a uniue geochemistry characteried by high salt and high concentrations n snoaffected regions, rivers, streams, and etlands are eeriencing increasing ionic concentrations, as indicated by chloride (l) concentrations, due to effects of the use of salt deicers on imervious surfaces hloride concentrations remain elevated yearround, indicating accumulation of salt in storage ones, and often eceed criteria for the rotection of auatic life A trend of elevated concentrations in urban auatic ecosystems is a lessernon attern, but has been detected in urban streams in multile regions omared to streams, less urban etland ater uality data is available

e assessed the ater uality functions of to urban stormater etlands in northeast hio by monitoring flos and solute concentrations at inflos, outflos, and etland interiors, and airing these data ith indicators of sediment biogeochemistry urface aters in these and other urban etlands routinely contained l and concentrations greater than mg, and sometimes l concentrations eceed toicity criteria for auatic life ur data suggest that trends of elevated l and concentrations in urban etlands may be more ronounced than in urban streams hrough these to case studies, e demonstrate that, in addition to novel urban geochemical stressors, etland design and construction, etland management, and climate conditions all contribute to the ater uality functions of urban etlands

r insmanostello is an Assistant rofessor in the eartment of iological ciences at ent tate niversity he is an auatic ecosystem ecologist interested in the effects of hydrology on nutrient biogeochemistry Her research aims to inform larger uestions about the ability of humans to manage, restore, and create ecosystems

ontact nformation auren insmanostello, eartment of iological ciences, ent tate niversity, o , ent, H, A

102 April 23-26, 2018 | Coral Springs, Florida, USA

TAL OILIT A RTTIO I COSTRUCT TLA SIT Anna S. Knox and hae H aer avannah iver ational aboratory, Aien, , A

he A etland treatment system () as designed to remove metals from the effluent at the A outfall at the avannah iver ite, Aien, he A effluent flos from a retention basin to a slitter bo, here it is distributed to four airs of cells in series before discharge to a stream ach treatment cell is a one acre etland that contains hoenoetu aornu (giant bulrush) and has a nominal hour retention time he urose of this research as to evaluate retention of the removed contaminants in etland sediment, and the otential remobiliation of these contaminants from the sediment into the ater column

enerally, the highest concentrations of u, b, and n ere found in the sediment from the first cell in each air of cells suggesting that most of the u, b, and n in the A effluent as bound to the sediment uicly euential etraction analysis as used to evaluate remobiliation and retention of u, b, n, n, and e in the etland sediment from the A emobiliation of metals as determined by the otentially obile raction () and metal retention by the ecalcitrant actor () he otentially obile raction () is an indicator of the contaminant fraction that has the otential to enter the mobile aueous hase due to changes in environmental factors such as H, h, temerature and others etals associated ith the are metals found in ater, echangeable, acid soluble, organic, and amorhous n and e oide fractions enerally, the values for u, n and b ere about hese lo values indicate that most of the removed metals ere in strongly bound hases and not easily etractable he values for e and n shoed an oosite trend – the values ere very high ie, about or more for e and for n hese high values indicate high desortion of e and n from the sediment and high solubility of their solid hases

he is a ratio of strongly bound fractions to the total concentration of elements in sediment (or the sum of all fractions) he is oosite to the ie, the indicates the virtually irreversible retention of metals by the solid hase his construct rovides an estimate of the ercentage of a contaminant resistant to remobiliation that is retained in the contaminated sediment he values ere about for u, n and b, indicating high retention of these elements in the sediment he values for e and n ranged from to above , much loer than for u, n, and b

r Anna ohia no is a ello cientist at the avannah iver ational aboratory () he has researched the biogeochemistry and geochemistry of metals and radionuclides in natural and contaminated soilssediments for years he has ublished more than scientific aers, boo chaters, and one boo

ontact nformation Anna ohia no, avannah iver ational aboratory, avannah iver uclear olutions, , Aien,

103 12th International Symposium on Biogeochemistry of Wetlands

RI LU CARO CARO STORA OF STUARI TIAL FLAT A SITS I ST COAST OF ORA Bong-Oh Kwon, unun oh, onn ee, Beo K, on eon Kh

rganic carbon and carbon stable isotopic ratio (δ

Contact Information: Jong Seong Khim, BENTHOS Lab., School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea

104 April 23-26, 2018 | Coral Springs, Florida, USA

CARO UT OF A ASTR ORT CAROLIA COASTAL FRSATR TLA FOLLOI OSORUS AITIO Sydni L. Law and nrue ee East Carolina University, epartment of Biology, reenville, NC, US

etlands store 30% of the earth’s soil pool of carbon. Fourteen percent of the world’s wetlands are found in the United States with 17% of those found in North Carolina (NC). Seventy percent of North Carolina’s freshwater etlands are pocosin peatlands. ocosins are non for their thic peat layers that store substantial amounts of carbon and other nutrients, particularly nitrogen. Since the 1920s, 50% of NC’s pocosins have been degraded for agriculture. This research is carried out in a pocosin peatland located on East Carolina University’s West Research Campus that drains into the Neuse and Tar Rivers, contributing to donstream river and estuary health by retaining large amounts of nutrients. The aim of this research is to determine ho these freshater ombrotrophic peatlands budget and store carbon hen the availability of phosphorus, the limiting nutrient, increases folloing different fertiliation treatments. CO concentrations ere collected from static greenhouse gas chambers at set time intervals to calculate CO flu, an indicator or net ecosystem metabolism and overall carbon storage in plant tissue and soil. bove and beloground biomass as also collected and burned to determine carbon lost on ignition and respective biomass allocation. Comparisons of biomass allocation and CO flu beteen treatments ere used to determine ho increasing phosphorus availability in the soil changes the overall carbon budget of this etland. Results sho that increasing the limiting nutrient in pocosins initially decreases the flu of carbon as CO out of the system under lo O fertiliation, but as O concentrations increase, so does the CO flu. emonstrating changes in carbon budgeting ithin a pocosin folloing nutrient application can provide insight into the fate of degraded peatlands and their potential influence on rivers donstream from them, such as the ones that occur along the southeastern Coastal lain of the U.S.

BIO: Sydni is currently finishing her Master’s of Biology research thesis at East Carolina University oring ith r. Enriue Reyes here she focused on ecology and biogeochemistry of etlands.

Contact information: Sydni L. La epartment of Biology, East Carolina University , N Hoell Science Comple, reenville, NC

105 12th International Symposium on Biogeochemistry of Wetlands

ASSSSI SIIFICAC OF IFFRT IROTAL RIRS IACT COASTAL LATIO CA I LOUISIAA Jing Liu Florida tlantic University, Boca Raton, F, US

n coastal ouisiana, it is certain that tidal wetlands already eperiencing subergence by sea level rise and associated high rate of land subsidence. With 12 per year Relative Sea evel Rise (RSR), 5,000 2 of wetlands has lost over the past century. When coastal arshes aintain their elevation relative to RSR, they could survive. n the other hand, if arshes vertical develop rate slower than RSR, they will gradually becoe suberged and convert to intertidal udflat or to open water. t is necessary to assess current and predict future rates of vertical buildup of coastal surfaces and wetland sustainability during the net century.

he processes of the capacity of coastal wetlands aintain a stable relationship with sea level is cople, any factors ust be considered in such an assessent. his study using epirical coastal surface elevation change data derived fro a networ in ouisiana, fro 2007 to 2017 to odel and assess the significance of different environental driver ipact on surface elevation change. Factorial analysis of variance was undertaen to develop a model that provided the best fit to the empirical data using surface elevation change and vertical accretion change as dependent variables. Water teperature, water level distance fro channel, percentage of vegetation and salinity were tested as independent variables. he aie nforation Criterion Corrected (Cc) score was used to select for the ost parsionious odel of independent variable interactions. Using tie series analysis to describe the iportant features of tie series pattern and eplain how the past affects the future to forecast the future values of the series. pplying the wetlands accretion odel to RSR to develop a landscape elevation odel that increase the integrity of sea level rise vulnerability assessent for coastal wetlands.

B ing iu is a second year h.. student in epartent of eosciences, FU. er ultidisciplinary research integrates environental science, S, reote sensing, statistical odeling, and achine learning techniues to better understand ipacts of accelerated sealevel rise on coastal systes.

Contact nforation ing iu, 777 lades Road, Boca Raton, F, US, 3332

106 April 23-26, 2018 | Coral Springs, Florida, USA

AICS OF SOIL ITRO ACCUULATIO FOLLOI TATIO RSTORATIO I A TICAL ARST CATCT Xin Liu1,2,3, han e,, an Ken, 1ey aboratory of groecological rocesses in Subtropical Region, nstitute of Subtropical griculture, Chinese cadey of Sciences, Changsha 10125, China 2uaniang bservation and Research Station for arst Ecosystes, uaniang 57100, China 3University of Chinese cadey of Science, Beiing 10009, China

he etreely fragile arst ecosyste in southwest China is one of the largest eposed carbonated roc areas (ore than 0.5 illion 2) in the world. Since the rain for reen and other ecological restoration proects have been ipleented in the late 20th century, uch tillage and pasture lands have been abandoned or changed fro crop to forest or other secondary vegetation states, which are accopanied by changes in ecosyste structure, processes and functions. owever, the dynaics of soil nitrogen (N) after vegetation restoration have not been thoroughly evaluated on the longter fiedpoint research in a arst catchent in southwest China.

n order to epand the scientific understanding of soil N accuulation after vegetation restoration, we analyed the data, which was recorded 10 years fro fiedpoint field eperient, to evaluate the dynaic of soil N seuestration after vegetation restoration in a typical arst catchent. We used 52 soil saples to easure the dynaics of soil nitrogen content (NC) and soil nitrogen density (N) on different vegetation restoration types (tillage converted to pasture, planted forest and ied plantation of pasture and forest, abandoned tillage, pasture converted to ied plantation of pasture and forest, abandoned pasture land, and unchanged tillage and pasture).

We observed that the overall soil N content across the catchent was increased significantly, but the overall soil N density had no obvious change in the topsoil after vegetation restoration. he overall soil N density only increased 0.3% with a lower rate of soil N seuestration after short ter vegetation restoration. Meanwhile, the ratio of soil carbon to nitrogen (C N ratio) in the overall catchent was increased significantly. ur study indicated that the recovery of soil N was slow, thus it reuires long ter vegetation restoration to recover the soil N in arst area.

B r. hang is an ssociate rofessor of . is research focuses on understanding the effects of huan disturbance and land use change on carbon and nitrogen seuestration and cycling of nutrients in terrestrial ecosystes.

Contact nforation Wei hang, ey aboratory of groecological rocesses in Subtropical Region, nstitute of Subtropical griculture, Chinese cadey of Sciences, Changsha 10125, China

107 12th International Symposium on Biogeochemistry of Wetlands

ARL IACTS OF RSTORATIO O IOOCISTR OF ITRTIAL OSTR RFS I T IIA RIR LAOO FLORIA Bryan Locher, a Hurtand, a haer uatic Biogeocheistry aboratory, epartent of Biology, University of Central Florida, rlando, F

lobally, oyster reefs have declined in nubers due to factors such as habitat loss, overharvesting, and disease. Within the northern ndian River agoon, F there are ongoing efforts to restore degraded reefs by providing substrate for oyster spat. lthough prior studies on restoration have focused on the effects of oysters on water clarity and nitrogen pollution in coastal water bodies, few have assessed the ipact of restoration on iproving biogeocheical cycling. his study ais to analye the effects of restoring an oyster reef on sedient and surface water nutrient cycling within the first year of recruitent and growth. Using a beforeaftercontrolipact design, four sedient cores and one surface water saple were taen on each of four dead, restored, and 3 naturalreference reefs to observe changes in physiocheical (bioavailable nutrients N3 , N , , and C total carbon, nitrogen and phosphorous pools bul density and sedient p) and icrobial (etracellular enyes) properties at tie points before restoration, and one wee, one onth, si onths and nine onths post restoration. reliinary results indicate that before restoration, naturalreference reefs display the highest aount of both bioavailable and longter nutrient pools as well as the lowest sedient p. ead reef sedients, with the lowest organic atter content, consistently had the highest bul density. Before restoration, the restored reef sedients show easureents siilar to the levels of dead reefs because they are both functionally dead – a pile of loose, disarticulated shells. Continuous onitoring over the net several onths will iprove the understanding of nutrient storage and cycling on oyster reefs and the tie after restoration reuired to restore biogeocheical function.

B Bryan ocher is a Master of Science student at the University of Central Florida in r. isa Chambers’ Aquatic Biogeochemistry Lab. His research interests include the biogeocheistry of coastal ecosystes and how to create cleaner, healthier coastal waters.

Contact nforation Bryan ocher, epartent of Biology, University of Central Florida, 110 ibra r., rlando, F 321

108 April 23-26, 2018 | Coral Springs, Florida, USA

COSTRUCT TLAS FOR SALTATR AUACULTUR ASTATR TRATT USI A FLOATI TRATT TLA IORACTOR SST A TLAS OIFI IT IORAAL LASTICS Cristina R. Lopardo, han, a th, and Hdetoh raawa eartmet o arie a coogica cieces oria u Coast iersity ort yers L A ergaes eta esearch ar oria u Coast iersity aes L A

Costructe etas are o to be eectie or euet treatmet o reshater ruo rom urba a agricutura areas hoeer itte is o about the eiciecy o utrietrich satater treatmet. o comare the utriet remoa eiciecy o costructe etas to tyes o mesocosms ere esige a oatig treatmet eta ith the haohytes embee i a oatig mat a a tyica costructe eta C ith sa a grae substrate. e set the goa o this stuy to eamie the eectieess o these costructe etas or euet treatmet o satater aquacuture asteater. e ate satater corgrass artna aternora i the etas a reare iish oodon rhoode i a recircuate aquacuture system. As a oe aroach bioegraabe astics ere itrouce i these etas as a carbo source to ehace a microbia utriet remoa rocess. oycaroactoe CL as icororate i the esig o the C through buria ithi the uer cm o sa hie CL as use as the reactor meium i a cose recircuatig bioreactor system ithi the . A tota o eight mesocosms gao oyethyee stoc ta . m . m . m ere reare our mesocosms a our C ith to o each haig bioegraabe astics a to cotros ithout. he eerimets ere coucte i eary summer i haig to eeig eets or the a our eeig eets or the C. A soe as use or moitorig o H oyge a saiity ithi the . Aboegrou at biomass as measure rior to eerimetatio a oce agai ater the cometio o eerimet. oot soi a astic sames ere coecte uo cometio o the eerimeta erio or highthroughut sequecig aaysis o microbia commuities. orgaic utriet cocetratios ere etermie through staar caorimetric methos or ammoia itrite itrate a hoshate. ur resuts shoe that the C ha a aerage itrate remoa rate o . . mg L ay or cotro a . . mg L ay or reicates ith a embee CL ayer oer the eerimeta erio etermie rom the ierece betee io a outo cocetratios. shoe that itrate remoa i ot occur or cotro mesocosms ith a aerage remoa rate o . . mg L ay or reicates ith the CLbioreactor equie . ur itrate measuremets shoe that C ha a arger remoa rate tha .

B Cristia Loaro is a irometa ciece .. stuet at oria u Coast iersity couctig research i the ie o eeoig utriet remoa techoogies through microbia remeiatio a costructe etas as art o her thesis or. he receie the orothy . ygh eoshi a Bair ouatio choarshi or her or.

Cotact ormatio Cristia Loaro oria u Coast iersity C B. outh ort yers L A

109 12th International Symposium on Biogeochemistry of Wetlands

FLUCTUATI SA LL A AITAT CA I STR AUSTRALIA Catherine E. Lovelock, a eer2, arn Ba, harn He4 and uth ee he iersity o ueesa t Lucia L Austraia. mithsoia irometa esearch Ceter geater A Austraia atioa iersity Caberra AC Austraia he iersity o ester Austraia Craey A Austraia. oash iersity eboure C Austraia.

ecet eiece iicates cimate chage a itesiicatio o the io outher sciatio has icrease ariatio i sea ee. Athough iesrea imacts are aticiate to arise rom the sea ee seesa associate ith cimate chage oe hae yet bee emostrate. tertia ecosystems o the ari oe icuig magroes a associate ecosystems o the high itertia sat ats are amog those ecosystems that are highy iuece by sea ee a thus they may be uerabe to sea ee ariabiity a etreme o sea ee eets. urig years o moitorig o a magroe orest i orth ester Austraia e ocumete to magroe iebac eets the most recet oe beig coiciet ith the argescae iebac o magroes i orther Austraia . iebacs i ester Austraia ere coiciet ith erios o ery o sea ee hich ere associate ith icrease soi saiiatio o aboe reeet ees eaig to caoy oss reuce magroe recruitmet a eeomet o coitios aorabe or cyaobacteria mats. ur stuy iicates that itesiicatio o a uctuatig sea ees aog ith other actors may iuece the baace betee magroe a high itertia sat ats ithi itertia ascaes o the ari oe.

BIO: Cath Lovelock’s research focuses on climate change and coastal plant commuities. he is a member o the teratioa Bue Carbo cietiic orig rou a is ea author o the etas chater or the ucomig reisio o the CC uieies or atioa reehouse as etories.

Cotact ormatio ro. Catherie Loeoc choo o Bioogica cieces he iersity o ueesa t Lucia L Austraia

110 April 23-26, 2018 | Coral Springs, Florida, USA

ITROUS OI ISSIO FRO ORL ATLAS Ülo Mander, aan rn,, ener, eee , He a, r reoa,, Kuno Kaa, artn addon, ara ruu, aa ruu niversit of artu artu stonia niversit of Birmingham Birmingham edish niversit of gricultural esearch me eden

itrous oide O is a poerful greenhouse gas and the main driver of stratospheric oone depletion e conducted a gloal soil and gas sampling campaign eteen ugust and ecemer folloing a standard protocol e sampled organic soil sites in regions across the tropical temperate and oreal climates ithin each region at least one site as estalished in a natural peatland and another one in an adacent drained area he hdrolog and trophic status of the natural peatlands ranged from groundaterfed samps and fens to omrotrophic peat ogs Land use varied eteen natural fen og samp or og forest and agricultural pasture ha field or arale ithin the sites locations folloed a groundaterdepth gradient as flues ere determined using the static chamer method at least three times ithin three das per location oil p O Ca and g and organic matter content ere determined from the collected samples In rench uiana fen samples the acterial and archaeal r genes and functional genes involved in nitrogen ccling nr nrK no no acterial and archaeal ao nH nr O acteria genes in soil ere uantified using uantitative C method

In all areas O emissions ere significantl higher in the affected sites than in the natural sites tatistical analses shoed a strong positive correlation eteen the O emissions and soil O content p hile soil moisture and ater tale level shoed a negative correlation ith O emission p in all sites – e found that the emissions can e predicted models incorporating soil nitrate concentration O ater – content C and temperature O emission increases asmptoticall ith O and follos a ellshaped distriution ith C Comining the to functions eplains of log O emission from all organic soils – ccordingl ecess soil O provided either through draining et soils or irrigating elldrained soils can – increase O emission orders of magnitude Comining soil O ith temperature eplains of log O emission cross the gloal sites tropical organic soils ehiited the highest O emission and thus should e a research and conservation priorit for reducing O emissions

rainage had a clear impact on the communities of nr nrK no archaeal ao and nH gene possessing microorganisms he structure of the communities as mainl related to different forms he acterial communit as more aundant p in the natural site hile the O production potential aundance of the nr genes as not different eteen the drained and nondrained sites O reduction potential aundance of no genes as higher p in the natural area here significantl loer mineral content and high groundater level ere detected

BIO: r ander is rofessor of hsical eograph and Landscape colog and ead of the epartment of eograph Institute of colog and arth ciences at the niversit of artu stonia is research and teaching are focused on landscape ecolog caron and nutrient ccling in etlands and catchment levels and ecological engineering constructed etlands: design and performance

Contact Information: lo ander epartment of eograph niversit of artu anemuise t artu stonia

111 12th International Symposium on Biogeochemistry of Wetlands

ROUS AS ISSIOS A CARO SUSTRATIO I ITRRAA RIC FILS A TLAS T RO LTA CAS Maite Martínez –Eixarch, are e, ntono adaa and ohan enne

30% of earth’s carbon but may emit 20

a

112 April 23-26, 2018 | Coral Springs, Florida, USA

FFCTS OF FLOOLAI RSTORATIO O ITRO A OSORUS AICS I ARICULTURAL ATRSS , eena ord, e ohnon, re oe, ddharth aena, and enateh erwade urue niersity est afayette 2 eooica urey eston

cess nitroen an hoshorus in receiin aters is associate ith hih nutrient inuts e fertiier foo an anima aste seae in atershes that are unsustainaby an intensiey manae for aricuture uses he maority of eort occurs urin hih fos that mobiie issoe nitroen an hoshorus as e as seiment an associate outants n uneeoe atershes fooains intercet this foo use creatin otima conitions for hysica an biooica retention to occur noic conitions in saturate sois or soi areates romote nitroen remoa ia enitrification but can aso cause reease of hoshorus from sortion sites that are maintaine uner oic conitions estoration or creation of rierine fooains has the otentia to imroe ater uaity hoeer unerstanin the cumuatie effects on nitroen an hoshorus ynamics is criticay neee ur oa is to etermine the functiona reationshis that eist beteen sources an sins of nutrients e enitrification hoshorus reease an hyroynamic an enironmenta riers e are usin the abashiecanoe ier confuence as a moe ecosystem to uantify fooain hyroynamics seimentation an carbon an nutrient fues yroynamic moein of the an anaysis of aiy fo freuency shos that urin the meian fo at east art of the fooain is inunate for 0% of the year 3 ays an the entire fooain is inunate 2% ays of the year his satia istribution of fooin is aso refecte in structura e soi nutrient content bu ensity an functiona e enitrification atterns he coariance beteen inunation soi roerties an enitrification suests that structura connectiity ays a stron roe in controin the istribution of fooain function his etaie unerstanin of the contros on bioeochemistry is criticay neee to uie manaement ecisions rearin acement an imact of restoration roects

r cian is an ssistant rofessor ith oer 0 years eerience in research esin an assessment of stream an etan restoration roects he interates fie eeriments an numerica methos to ientify contros on bioeochemica rocesses an otimie acement of ecooicay base manaement ractices in aricutura an urban atershes

ontact nformation ara cian ricutura an iooica nineerin urue niersity 22 niersity treet est afayette 0

113 12th International Symposium on Biogeochemistry of Wetlands

TA ISSIOS FRO OT TLA A ULA TRS ACROSS A FLOOI RAIT , ott t, and Katherne ae mithsonian nironmenta esearch enter eater 2ohns oins niersity atimore

tuies of fues in forests hae traitionay focuse on soi surfaces ith etan forests actin as net sources an uan freey raine forests as net sins t is increasiny cear that is aso echane across eaes branches truns an ooy ebris but it is not cear hether these surfaces are uantitatiey imortant to forest buets because of a ac of emirica ata e inestiate the reatie imortance of echane across sois an tree stems as a function of secies time an ater tabe eth easurements of an 2 fu ere mae from trees 0 m aboe roun an sois oer to roin season on 32 iniiuas an secies arrane across a 0 m uantoetan forest transect at the mithsonian nironmenta esearch enter aryan s eecte uan sois ere net methane sins hie transitiona an etan sois ere net sources meanse 65±6, 7±25, and 190±123 μg m2 soi h resectiey y comarison 2 tree stems ere neary aays net sources, with emissions of 69 ±13, 181 ± 55 and 568 ± 175 μg m stem h in uan transitiona an etan habitats resectiey ethane fues ere not consistenty obsere from 20 of the tree stems but eery iniiua reease fu at some oint urin the stuy e concue that tree stems hae a meaninfu effect on the buets of forests – both etan an uan – that has been arey oerooe

at eonia is enior cientist at the mithsonian nironmenta esearch enter an an ecosystem ecooist ith eertise in carbon cycin an reenhouse as fues in etans an forests as they reate to oba chane e is a eo of the cooica ociety of merica an the oi cience ociety of merica

ontact nformation at eonia mithsonian nironmenta esearch enter ontees harf oa eater 203

114 April 23-26, 2018 | Coral Springs, Florida, USA

SUSTAIAL SOLI LAC OSORUS A ITRO I LASCAS IT TLAS A TLACULTUR , and BnBn an,

— —

ooa nneern

115 12th International Symposium on Biogeochemistry of Wetlands

T ROL OF RII FFCTS O T CORSIO OF LU CARO TO CO I T COASTAL O , ard, reano, u, aah, ra, orne, auhn, Banh

systems. She received her PhD from the University of Florida’s Soil and Water Sciences Department in soil microbial ecology.

116 April 23-26, 2018 | Coral Springs, Florida, USA

UTRIT RICT ALTRS LU CARO OOLS A ROCSSS , on Barer, ea or, ohua aan, atn Bauer, eohean, ranne eeh, aa a, ae eer ryn ar ollege ryn ar P US Smithsonian nvironmental esearch enter dgeater D US emple University mbler P US

lobal change factors are non to indce feedbacs on primary prodction that drive ecosystem processes inclding carbon seestration in tidal salt marshes. lthogh there has been an emphasis on the inventory of ble carbon pools and processes little attention has been given to the potential role of changing fondation species genetic diversity and genetic identity on carbon cycle processes. n a longterm nitrogen enrichment eperiment in assachsetts D e have fond that ntrient polltion acted as an environmental filter that redced intraspecific genetic diversity altered genetic identity and altered plant traits e.g. floering phenology rootshoot ratio of the fondation species artna aternora.

o evalate ho these changes in fondation species alter ble carbon pools and processes e measred carbon pools biomass allocation and carbon cycle processes for to years in paired nitrogenenriched and reference salt marshes. We fond that genetic changes in ntrient enriched ecosystems ere associated ith redctions in belogrond biomass altered phenology and increased ecosystem respiration in comparison to paired reference ecosystem. edced belogrond biomass allocation and enhanced ecosystem respiration have reslted in redced soil carbon pools at depth. r data sggest that genetic changes in fondation species from factors sch as ntrient enrichment may create cascades that accelerate changes in ecosystem processes to alter the ble carbon fnction of etlands.

BIO: Dr. Mozdzer is an assistant professor in Bryn Mawr’s Biology Department. He has nearly 20 years’ experience researching coastal etlands. is research has been focsed on the effects of global change on greenhose gas fles carbon storage plant ecophysiology genetic diversity of fondation species and invasive species.

ontact nformation homas oder ryn ar ollege . erion ve ryn ar P

117 12th International Symposium on Biogeochemistry of Wetlands

ICROSCAL RO ROI ASURTS OF CAOACTRIAL LOOS I SOUTST FLORIA LAS , hard Barteon , ean eene, awe B ettt, Harua raawa, r randt, hae aron and Hdetoh raawa lorida lf oast niersity ort Myers 2Marine aoratory anielaptia onseration ondation aniel

yanoacterial looms are a gloal prolem ecase of the increased flx of nitrogen and phosphors and other limiting ntrients into laes and coastal waters. yanoacterial looms are of special concern to hman health as stdies hae shown that they can prodce toxins or other sstances that lead to illness. long with ntrient management chemical treatments to control harmfl algal looms hae een sed inclding copper slfate iological treatment y se of and recently the se of hydrogen peroxide. he tilization of hydrogen peroxide to control cyanoacterial looms is promising ecase hydrogen peroxide does not last long and is degraded into water and oxygen. In high concentrations howeer hydrogen peroxide is toxic to aatic organisms and an nderstanding of natral hydrogen peroxide concentrations is necessary for ftre water management. he aim of this proect was to collect aseline data of hydrogen peroxide in sothwest lorida laes. e also examined iological responses of cyanoacteria to hydrogen peroxide exposre.

Hydrogen peroxide was antified y ferros ion oxidation in the presence of xylenol orange and y sing hydrogen peroxide microsensors. e compared these two methods in terms of detection limits and linearity and fond that oth methods correlated with each other and are interchangeale. Or initial sensor wor showed that the concentration of hydrogen peroxide changes icly in cyanoacterial cltres it was prodced nder the light condition while it declined nder the no light condition. Based on this oseration we hypothesized that preios measrements of hydrogen peroxide in literatre constantly nderestimated the concentration of hydrogen peroxide ecase it wold e degraded dring the transportation of water from the field to the laoratory. o confirm this hypothesis we compared two srface water samples one was collected from the field and transferred to the laoratory with le ice and the other was treated with 0.2m poresize filters at the field and transported in the same manner. he reslts spported or hypothesis and showed that the concentration of hydrogen peroxide was consistently higher in the filtered water samples than the normal nonfiltered condition water samples. iteratre data sggested that the concentration of hydrogen peroxide is higher in srface water than the deeper water in laes. Howeer no sch measrements hae een taen in sothwest lorida. In addition nothing is nown aot microscale distritions of hydrogen peroxide in srface lae water. hs we sed the hydrogen peroxide microsensor to examine the millimeter scale distrition of hydrogen peroxide in thin srface lae water. hese measrements were applied nder cyanoacteria loom conditions and compared with normal conditions. he lae srface microlayer water was collected and sed for standard ntrient and chlorophyll analyses along with iron measrements. e isited two cyanoacterial looming sites Deitt ond in aniel Island and ae an arlos in ort Myers and determined ertical hydrogen peroxide profiles in these laes approximately 2 m deep. In Deitt ond the hydrogen peroxide concentration ranged etween 0. to . M and the maximm concentration was osered at the srface. imilar profiles were otained from ae an arlos t the concentration was higher and ranged from 2. to . M. he microprofiles 00 mm showed steeper hydrogen peroxide gradients and ranged from .2 to 2. M in Deitt ond and . to 20. M in ae an arlos. he concentration of hydrogen peroxide was higher at the loom sites than at the control sites and higher at the location in the sn than the location in the shade. ll together we fond a high natral acgrond leel of hydrogen peroxide in sothwest lorida laes and a significant contrition y cyanoacteria in hydrogen peroxide dynamics.

BIO: Luka Ndungu, is pursuing master’s degree in Environmental Science at Florida Gulf Coast University. He is studying the interaction etween cyanoacteria and hydrogen peroxide in aatic ecosystem.

ontact Information: a dng lorida lf oast niersity 00 Bld. oth ort Myers

118 April 23-26, 2018 | Coral Springs, Florida, USA

SASOAL A URRICA IRA FFCTS O T ROLOIC RI OF A COSTRUCT URA STORATR TRATT TLA COL I SOUTSTR FLORIA , auren rth, and a th Everglades etland esearch ark, Bayshore rive, Florida Gulf Coast University, Naples, FL, US

Freedom ark, a ha park constructed in Naples, Florida in , includes a .ha stormater etland treatment system for improving the ater uality of the donstream impaired Gordon iver and estuaries that flo into the Gulf of eico. he atershed includes , ha of a highly uranied Naples metro area. he goals of the stormater etlands are to improve the ater uality, reduce peak flos discharging into Gordon iver and further to Naples Bay, and provide an aesthetic ater park for the pulic. Understanding the hydrology of the system is a critical component in understanding the overall functioning of its design, and in determining if the original goals of the system are eing met. hydrologic udget as summaried into monthly udgets eteen ugust , and Octoer , , hich included oth et and dry seasons. For the month study period, average surface ater pumped into the system as cm eek the average surface outflo as cm eek. uring this period, average precipitation inflo as . cm eek, hile the average evapotranspiration rate as . cm eek. he average ater level at the outflo asin decreased gradually throughout the dry season from Novemer , to ay , , then rapidly spiked . m during the eek of une , hen the et season egan. he system also received an additional pulse of cm during Hurricane Irma on Septemer , . revious studies have found that the etland system acted as a net sink of nutrients, as as intended y its original design. Our stormater pulsing study during Hurricane Irma shoed that after an initial inflo flush due primarily to rainfall, total phosphorus and total nitrogen concentrations ere higher at the outflo than at the inflo for several days efore returning to normal conditions. Similarly, the mass of nutrients leaving the system as and higher for N and , respectively, than the mass entering the system during this day period during and folloing the hurricane. he design of the inflo ith pumps rather than natural ditch overflo also meant that the rainfalldominated inflo as much less than it ould have een during such a dramatic hydrologic pulse. e also found that the dissolved oygen in these etlands, hich as normally hypoic elo mgL, riefly increased for days to mgL efore returning to the typically lo oygen conditions. Samples taken at the inflo and outflo of the system for several other storm events throughout the et season ere also analyed for their effects on ater uality.

BIO: aylor Nesit is a secondyear graduate student in the asters of Environmental Science program at Florida Gulf Coast University. She orks as a Graduate esearch ssistant at the Everglades etland esearch ark in Naples, Florida. ost of her research is focused on the ecosystemfunctioning of created stormater treatment etlands at Freedom ark.

Contact Information: aylor Nesit, Everglades etland esearch ark, Bayshore rive, Naples, FL,

119 12th International Symposium on Biogeochemistry of Wetlands

RSISTT SALTATR ITRUSIO ALTRS COSST CARO CCLI I TIAL FRSATR ARSS COARISO OF RSULTS FRO AIULATIOS I IRIIA A SOUT CAROLIA , oe Hoten, on oon ee, irginia Commonealth University, ichmond, , US Current address: Florida International University, iami, FL US

Saltater intrusion, hich is driven y a variety of mechanisms including sea level rise, changes in precipitation, and anthropogenic hydrological modifications, can cause saliniation of tidal freshater etlands. his saliniation alters one of the defining properties of tidal freshater etlands, namely their lo salinity, and leads to changes in the soil physicochemical environment, microial communities, and plant processes. Collectively, these changes to the ecosystem may cause alterations to important ecosystem services including primary production and caron seuestration.

e have conducted longterm multiyear n tu salinity manipulation eperiments in tidal freshater marshes in irginia and South Carolina. hese sites have similar ackground salinity levels, typically ., ut differ in their soil characteristics, hydrology, and plant communities. t each site, additions of rackish ater to the surface of the marsh have increased poreater salinities from freshater to oligohaline levels. e have made a suite of measurements at each site, including poreater chemistry, marshatmosphere flues of CO and CH, and soil CO and CH production potentials. uring the first year of manipulation at the irginia site, e found that saltater intrusion decreased gross primary production and ecosystem respiration y ithin .–. months of eginning the eperiment. In contrast, at the South Carolina site there as only a transient decrease in CH emissions and no change in CO flues eteen the marsh and atmosphere during the entire first groing season of manipulation. uring the second and third years of manipulation, oth sites ehiited sustained and sustantial reductions in rates of gross primary production, respiratory CO emissions, and CH flues to the atmosphere. espite the eteensite consistency in ho these CO and CH echanges respond to saltater intrusion, there as sustantial interannual and sitetosite variaility in ho net ecosystem production a proy for the rate of ecosystem caron storage responded to saltater intrusion. Sometimes, saltater intrusion did not affect rates of net ecosystem production ut other times net ecosystem production as reduced y due to saliniation.

lthough each of our n tu eperiments has lasted for multiple years, the responses e have captured still represent the initial disturance stages of saliniation. It remains to e seen ho these ecosystems ill respond over even longer time scales as salttolerant plants move into today’s tidal freshwater wetlands and microbial communities shift in response to saliniation. Because caron accumulation is a key contriutor to soil accretion in tidal freshater etlands, these saliniationdriven alterations to the etland caron cycle have implications for future etland sustainaility in the face of rising sea levels and increasing salinity at the head of estuaries.

BIO: r. Neuauer is an associate professor in the epartment of Biology at irginia Commonealth University. His research focuses on etland iogeochemistry and microial ecology, ith an emphasis on ecosystem responses to environmental change. He has etensive eperience in tidal freshater marshes, having orked in these fascinating ecosystems for years.

Contact Information: Scott C. Neuauer, epartment of Biology, irginia Commonealth University, ichmond, , US

120 April 23-26, 2018 | Coral Springs, Florida, USA

RSTORATIO OF IOOCICAL CARACTRISTICS TROU ACTI AAT OF T UTRITRIC RLAS , ar oo, hae anna, and hrta we oth lorida ater anaement istrict est alm each

or decades of elevated phosphors loads to the historically oliotrophic verlades cased a reime shift from the ride adu aaene rantandsloh landscape to lare areal epanses of cattail ha donen ers o accelerate the recovery of these impacted areas we condcted a larescale n tu stdy comprised of fifteen ha plots to test or ability to rehabilitate cattail areas by creatin an alternative sbmered aatic veetation open reime e hypothesied the open environment wold be more oyenated with hiher wildlife vale bt there was a concern that a larescale veetation manaement action wold eacerbate conditions in this already enriched landscape rin the first for years floc levels were sinificantly elevated in the created open verss cattail dominated plots owever more recently years post creation floc concentrations have decreased in the westernmost plots while remained somewhat elevated in the easternmost plots here is also a dramatic increase in ash content from to in open compared to cattail plots indicative of hiher mineral enrichmentprecipitation in the westernmost plots here are several mechanisms that liely eplain this differential response incldin location of the plots relative to a northsoth airboat trail ths inflencin how srface water flows from inflow clverts affect landscape bioeochemistry the enerally reater flows from the westernmost inflow strctre contribtin to hiher mineral loads in the western plots and the sbtly different hydroperiods and water depths istin hydroperiods and depths contribted to more rapid rerowth of emerent plants sch as cattail and more oranic conditions in the plots east of the northsoth airboat trail n contrast the combination of hiher minerals and shallower depthsshorter hydroperiods at the western sites spported reater metabolism which in trn reslts in reater calcim carbonate precipitation and potentially cappin of the leacy his stdy hihlihts the importance of linin chemistry hydroloy and bioloy and nderstandin their feedbacs when desinin restoration proects n addition the importance of eaminin lonterm as well as shortterm responses when considerin whether an active manaement treatment is sccessfl and sstainable

r ewman is a enior cientist and ection eader of the arsh coloy esearch rop with over years of eperience condctin research and restoration proects in the verlades

ontact nformation e ewman verlades ystems ssessment ection oth lorida ater anaement istrict elvedere oad est alm each

121 12th International Symposium on Biogeochemistry of Wetlands

ICROIAL COOSITIO OF RLAS STORATR TRATT ARAS IS LI TO SULFUR CCL , Bhoa, uoto, eer, and K edd oil and ater ciences epartment niversity of lorida ainesville

he verlades stormwater treatment area flowways s were desined to remove ntrients especially phosphors from aricltral rnoff before it enters the verlades rotected rea n the years since the constrction of the s many stdies have focsed on ways to optimie the efficiency of this system owever little wor has focsed on the microbial composition of the s and the role of microbes in ntrient cyclin amplicon srvey was sed to stdy the slfr cycle in the e are able to detect enera of slfateredcin bacteria mae p to of total relatively abndance belon to families eslfarclaceae and eslfohalobiaceae eslfovibrionaceae eslfobacteraceae yntrophaceae and yntrophobacteraceae all belon to eltaproteobacteria eslfarclaceae and eslfococcs are the most abndant families made p abot of all slfateredcin bacteria lfateredcin bacteria are hihly abndant in floc ras and pre lfroidiin bacteria are most abndant in water and floc sample mae p to of the relative abndance ammaproteobacteria is the most abndant class mae p to of all slfroidiin and is ite diverse with enera with heotrichaceae and alothiobacillaceae are the most abndant families he second most abndant rop is psilonproteobacteria and the third one is etaproteobacteria lfateredcin and lfr oidiin bacteria mae p a sbstantial portion of the microbial commnity in both and s s sch these two rops may eert control over phosphate availability in aatic systems and deserve indepth investiation

ontact nformation rs anh yen niversity of lorida ainesville

122 April 23-26, 2018 | Coral Springs, Florida, USA

UTRIT A SIT IUTS CA SOIL STRUCTUR A IOOCISTR I FLOOLAI COSSTS A CROSSSTU STSIS , Hu, d hen, Ken Krau, ara an, an Kroe, ott nn, ae ee, Krtn o, and hanwoo hn eoloical rvey ydroloicalcoloical nteractions ranch eston irinia ational ar ervice lastaff riona eoloical rvey etland and atic esearch enter afayette oisiana rde niversity est afayette ndiana eoloical rvey aton oe oisiana atic nalysis and onsltin orehead ity orth arolina eoloical rvey roect aboratories ranch eston irinia hamplain ollee aterbry ermont eore ason niversity airfa irinia

e synthesied measrements of inpts and internal cyclin of sediment and ntrients made across mltiple stdies of floodplains in the eastern sotheastern and soth central nited tates e specifically tested if floodplain inpts of sediment and ntrients and soil bioeochemical process rates differ amon landscape settins and amon natral restored and created floodplains e also tested if inpts chane floodplain soil strctre and ntrient mineraliation rates

ates of sedimentation and associated particlate and inpts varied amon physioraphic provinces with enerally increasin rates from hiher to lower radient landscapes from montains to the sea oil and net mineraliation rates and trnover rates also varied across landscape settins trient mineraliation rates were eplained by a combination of material inpt rates and soil characteristics with ntrient inpts havin the most inflence on soil and mineraliation and trnover whereas soil characteristics were more important for trnover nitrification and ammonification he ntrient concentrations in deposited sediment and nderlyin soil widely divered at locations with little sedimentation and convered where there was reater sedimentation inally spatial covariation in oranic and mineral sedimentation amon measrement locations identified averae allochthonos and atochthonos sedimentation rates of and m yr respectively n smmary this mltistdy synthesis of floodplain ecosystem ecoloy fond that reater inpts of sediment and particlate and dissolved and associated with increasin hydroloic connectivity chaned soil characteristics enhanced trappin and stimlated bioeochemical cyclin in floodplains

r re oe has been a esearch coloist with the eoloical rveys ater ission rea in eston irinia since where he leads the etland cosystem Ecology & Biogeochemistry Laboratory (WEEBL). Dr. Noe’s research centers on wetland ecosystem ecoloy focsin on the interactive inflences of eomorpholoy hydroloy climate and bioloy on nitroen phosphors and carbon bioeochemistry in flvial ecosystems as well as plant commnity ecoloy and restoration ecoloy

ontact nformation re oe eoloical rvey ational enter eston irinia

123 12th International Symposium on Biogeochemistry of Wetlands

RI LU CARO ILOT STU OF ORAIC CARO A ITRO STORA I STUARI TIAL FLAT SIT ORA , Bonh Kwon, onn ee, Beo K, on eon Kh chool o Earth and Enironmental ciences & esearch nstitte o ceanograhy eol National niersity eol eblic o orea

ecently orean goernment has highlighted the alable role that coastal and marine ecosystems lay in seestering organic carbon and nitrogen. oweer the ariation in rates o carbon and nitrogen storage and the actors controlling storage hae oorly nderstood in orean tidal lat. his stdy aimed to ) ealate the carbon and nitrogen storage in worldwide scoe o coastal and marine ecosystems ) assess the carbon and nitrogen storage caacity in the orean mdlat by analying organic matter bl density total nitrogen (N) sediment organic carbon (SOC), and carbon stable isotopic ratio (δ) in the sediment. ediments were cored rom arios tyes o tidal lat sch as anghwa Boryeong aemangem coast emrier Estary and Nadong delta. eslts showed that reios global stdies generally reorted that mangroes had the highest carbon and nitrogen storage caacity ollowed by salt marshes and mdlats. n ield stdy o orea more egetated sediments in anghwa hae higher and N densities (. g ha and . g N ha) while recent reclaimed sediments in aemangem hae the lowest and N densities (. g ha and . g N ha). he and N stocs tended to decrease rom the egetation mdlat to the bared mdlat in the order more egetated mdlat ewer egetated mdlat bared mdlat. arbon storage caacity in mdlats generally showed smaller stocs comared with mangroes howeer it was also a alable sin or carbon storage becase the amont was estimated aroimately as . note reslts indicated that the area where lant detrits was slied as carbon and nitrogen sorce showed more and N stocs comared to dried area by reclamation. erall the mdlats layed an imortant role as an organic carbon and nitrogen reseroir.

B r. Noh is a h.D stdent o eol national niersity orea. is maor is marine benthic ecology and he is a roect researcher or the irst BLE BN roect ocsing on organic carbon storage and seestration o costal area orea. e is also stdying mdlat restoration and coastal ecology and has articiated in more than roects dedicated to resering and restoring mdlats.

ontact normation ong eong him BEN Lab. chool o Earth and Enironmental ciences & esearch nstitte o ceanograhy eol National niersity eol eblic o orea

124 April 23-26, 2018 | Coral Springs, Florida, USA

IOO ARO FORST IOASS STIATS COUIT STRUCTUR A CLASSIFICATIO I T IRIA IR LTA chool o eosciences niersity o Edinbrgh nited ingdom

angroe orests are imortant coastal ecosystems becase they roide cltral roisional and reglatory serices bt they are threatened by eloitation rbaniation and sea leel rise. Nigerian mangroes are hotsots o oil olltion deeloment deorestation and inasie secies esecially in the Niger Delta. ence mangroes in Nigeria shold be stdied to nderstand the eects o these land se changes on the biomass desite research challenges.

assessed Niger Delta mangroe orests in terms o aboegrond biomass commnity strctre and how arios economic actiities in these regions aect them. lassiication o my stdy region was also done to dierentiate mangroes rom the inasie secies Nia alm and the relationshi between biomass and radar bacscatter was established. sare lots in sites were samled in states o the Niger Delta grond control oints were also collected across the Niger Delta or classiication sing otical and radar data rodcts rom L L and entinel.

nitial reslts show that a total o . ha o mangroe area was samled with mean B o . . t ha−. n oerall accracy o and aa coeicient o . was deried rom the classiication o the stdy area sing maimm lielihood method. oweer the commission o Nia alm was abot andcanbeimroed. he relationshi between radar bacscatter and lot estimates o B showed the highest sare ale o and ale . rom a combination o the entinel radar and L L data. he site with the highest biomass was ond in the rotected site lots and the lowest was ond on the site were rbaniation was actiely taing lace. DB sie distribtion and biomass contribtion also gae eidence to this also. he reslts show that the eects o eloitation in the Niger Delta mangroe orests are nderestimated and nderreorted hence the need or an dated mangroe biomass monitoring the Niger Delta. new mangroe biomass ma is ossible in the Niger Delta based on these reslts which will be imortant to staeholders in lanning mangroe conseration.

B hweba Nwobi is a yong scientist secialiing in mangroe carbon stoc ecosystem nction and restoration o mangroe orests in Nigeria.

ontact normation hweba Nwobi. niersity o Edinbrgh lobal hange esearch nstitte chool o eosciences oom rew Bilding ings Bilding Edinbrgh E nited ingdom

125 12th International Symposium on Biogeochemistry of Wetlands

O UTRITS ITRACT IT STRSSS SUC AS FLOOI A SALIIT TO AFFCT TLA LAT ROT A LAF TISSU STOICIOTR Loisiana tate niersity gricltral enter Baton oge L

Ntrients oten limit lant deeloment and rodctiity inlence the otcome o cometition among lant secies and inlence herbiore behaior throgh their eects on lant tisse chemistry. Liewise stresses sch as looding and salinity oten limit lant rodctiity and inlence the otcome o cometition among lant secies. he relationshi among lant growth ntrients and a stress can be classiied according to whether growth is dominated by the stress or by the ntrient and whether the stress and the ntrient act indeendently or interact. here are or ossible otcomes a the ntrient and stress act indeendently with the stress dominating growth the ntrient and stress act indeendently with the ntrient dominating growth the ntrient and the stress interact with the interaction dominated by the stress and d the ntrient and the stress interact with the interaction dominated by the ntrient. Eeriments sing ntrient aailability looding stress or salinity stress and a wetland grass (artna aten (iton) hl.) or a wetland tree (aodu dthu (L.) ich.) indicated that a limiting ntrient did not increase growth when the stress was high. his otcome relected the third ossible otcome aboe ntrients and stresses interacted with the interaction dominated by the stress. his otcome also relected rinciles o ecology and toicology increasing aailability o a limiting ntrient increases growth and eerything inclding ntrients is a toin the dose determines whether or not something is toic. When lant growth is limited by stress the only resonses to ntrients are noresonse or a toic resonse which can aear to be a tiing oint i one is looing or a tiing oint.

tress alone altered the N ratio o leaes. n aten the eect o salinity stress on lea tisse N was small enogh that it cold be acconted or when interreting N ratios. n dthu howeer lea tisse N ratio cold not be sed to comare ntrient aailability among trees becase looding cased a bigger dierence in N ratios than ntrient aailability. n dthu g in irstyear leaes was increased by looding stress and decreased by ntrient aailability resmably when roots were smaller and limited to the srace o ots. oweer g in secondyear leaes increased with ntrient aailability when ntrient aailability also increased root biomass and resmably allowed roots o een drained treatments to enetrate deeer to redced soil where methylation o g is more liely. ch comleity might comlicate redicting landscae hotsots o mercry methylation.

B ndy Nyman is a roessor o wetland wildlie with more than eerreiewed blications and years o eerience woring with wildlie managers and wetland restoration agencies.

ontact normation ohn ndrew Nyman chool o enewable Natral esorces L Baton oge L

126 April 23-26, 2018 | Coral Springs, Florida, USA

A ORI OF LOAL A RIOAL SA LL RIS ROCTIOS AS A TRS oth lorida Water anagement District (WD) West alm Beach L

nderstanding the imacts o tre sea leel rise on coastal wetlands and inrastrctre is imortant or lanning o restoration and resiliency eorts. While data rom tide gages and satellite altimetry roide alable inormation on historical rates o sea leel rise recent wor clearly indicates that these rates are dynamic and liely to change in the tre de to a ariety o actors. oled with eectations o an accelerated increase o global temeratre sggested by climate models a signiicant acceleration o global sea leel rise cannot be eclded rom consideration in the tre lanning o coastal inrastrctre. tre sea leels in coastal regions will be rther modiied by regional and local actors sch as ertical land moement dynamic eects o ocean circlation and wind atterns and the changes in graitational eects o melting ice rom reenland and ntarctica. Nmeros entities hae attemted to roect sea leels both globally and regionally. tre increase in mean sea leels along coastlines will also reslt in signiicant changes to the reency and magnitde o sea leel etremes. his resentation will roide an oeriew o nmeros global and regional sea leel rise roections modeling the magnitde and reency o sea leel etremes and the otential imlications o increasing sea leels on coastal roects located in both natral and bilt enironments.

B Dr. beyseera is the technical lead or climate change and sea leel rise at WD. e is a coathor o three national reorts on global and regional sea leel roections and is a reciient o the Norman edal rom the merican ociety o iil Engineers.

ontact normation ayantha beyseera oth lorida Water anagement District n lb oad West alm Beach L

127 12th International Symposium on Biogeochemistry of Wetlands

ARIATIO I CARO STORA A UTRITS I ARO ATS ACROSS URTO RICO , odd row,, hae o,, ohn eeder, oeh oa, ohn Kono,, ae oururean,, enne era,, ara anto,, and atana Barreto, nstitte o Water and Enironment lorida nternational niersity iami L Deartment o Biology lorida nternational niersity iami L Deartment o Earth and Enironment lorida nternational niersity iami L Deartment o Enironmental cience niersity o oth lorida t. etersbrg L niersity o erto ico io iedras erto ico E enter or atic hemistry and Enironment lorida nternational niersity iami L

angroe ecosystems lay a ital biogeochemical role at the continental – estarine bondary storing carbon and ntrients and seestering contaminants. et the ability o mangroes to maintain ecosystem nctions is ncertain gien accelerated sealeel rise and landse changes that alter rodctiity and storage. dditionally we lac an nderstanding o how hydrogeomorhic conditions and hysiograhic contet inlence biogeochemical cycling and carbon storage. cross the island o erto ico mangroe orests are ond across a wide range o climate (moisttroical to arid) and geomorhic (igneos to sedimentary) settings. hese ariables inlence orest rodctiity amont o terrigenos sediment inl and orewater salinity.

n we embared on a ilot roect to docment dierences and similarities in carbon storage and ntrient accmlation in three coastal locations in erto ico inones on the north coast obos Bay on the sotheast coast and nta itahaya on the sothwest coast. wo cm dee cores were collected in each location. otal nitrogen (N) hoshors () organic () and inorganic carbon () and ercent organic matter () were determined down core at cm interals in the to cm and cm thereater. adiometric dating sing b isotoes is being condcted to generate sediment accmlation rates.

Estimates o ercent organic matter rom loss on ignition were mch lower on aerage in the cores rom the north side o the island comared to those o the soth side ( to s. to ). imilar trends were obsered or and N. rends in were obsered or all cores with increasing oer time rom to gg to more than gg at the srace. oweer the timing and abrtness o increases in ary by location and are liely attribtable to mltile henomena inclding changes in stream agricltral intensity and climatic conditions that wold bring increasing amonts o terrigenos materials into the estarine enironment. dditionally eriodic distrbance rom hrricanes liely lays a role in obsered ariation down core. inally we comare or initial reslts to those o the wider aribbean region inclding soth lorida.

B Dr. grca is a ostdoctoral associate with a degree in Earth ystems cience rom lorida nternational niersity. he has oer years o eerience in ecological research in the Eerglades and the lorida eys.

ontact normation Danielle E. grca nstitte o Water and Enironment lorida nternational niersity .W. th treet iami L

128 April 23-26, 2018 | Coral Springs, Florida, USA

T ROOTS OF LU CARO I ARO FORSTS T FFCT OF SICAL SOIL RORTIS O STILT ROOT LOT I and atherne oeo

hohora toa

129 12th International Symposium on Biogeochemistry of Wetlands

IACTS OF SIT RI O OSORUS AICS OF A RSTOR RIARIA TLA and an tenan

130 April 23-26, 2018 | Coral Springs, Florida, USA

TOS OF SULFUR AALSIS I TLAS STUIS A ALICATIOS TO RCUR IOOCISTR , nne Bate, an he, nne ott, ar t, eore en, Brett oun and ad Kreenhot

Sulfur isotopes have been used to examine sources as well as redox cycling. For example, δ ction and methylmercury production in wetland soils. An approach using both δS and δ

131 12th International Symposium on Biogeochemistry of Wetlands

TRASATI T FFCTS OF SALL RIS I URA SSTS TO T COASTAL COSST ITRFAC ,, ar ar, au uan, oa ard,, oear on, dward h, aea ether

he oete o th tud were to haratere eooa reeant te o atwater etratae ontanant that are nora ound n uran o and erou urae and deterne the antude o oaton that an e eeted under hort ter nundaton u trenth eawater

132 April 23-26, 2018 | Coral Springs, Florida, USA

USI T TO ASUR IOAAILAL TALS I A COSTRUCT TLA TRATT SST and nna Kno

We used DGT probes to measure a contamination gradient and bioavailable metal fractions in the A‐01 wetland

aua horoo

133 12th International Symposium on Biogeochemistry of Wetlands

“ACCIDENTAL” URBAN WETLAND BIECEICAL RCEE IN UNEECTED LACE , an B r, eter roan 1ace niversit ew or A Ariona tate niversit Tempe A A it niversit of ew or ew or A

“Accidental” urban wetlands are environments formed not through deliberate restoration or management activit but as a result of land use and water infrastructure decisions b municipalities These wetlands are often formed in abandoned industrial residential or commercial areas that are low‐ling in the landscape where overland flows from storms and municipal water use can accumulate and support wetland soils and plant communities Their positioning within the urban matri means that accidental wetlands have the potential to both receive and modif materials from surrounding urban areas Abandoned industrial sites have been estimated to occup 000 m in cities but the function and capacit of pollutant removal in unrestored urban wetlands has been little studied esearch we have conducted in both the northeastern ew erse eadowlands and the southwestern alt iver in hoeni Ariona demonstrates that accidental wetlands tend to receive high volumes of nutrient‐enriched municipal storm and rainwater and demonstrate the capacit for high levels of nutrient removal

ittle is nown about how management and design intention or interventions influence most wetland functions as compared to no interventions or design cosstems such as created wetlands which are deliberatel constructed to support specific biogeochemical processes tpicall include structural characteristics that mimic those of natural sstems where contaminant processing is high Design and management of vegetation communit soils and hdrolog is meant to facilitate particular wetland functions eg nutrient removal microbial habitat We argue however that accidental wetlands ma in some cases provide a greater variet of functions than designed urban environments because the latter can be overdesigned for a limited set of specific functions Although their soils and hdrolog differ greatl from native and constructed wetland sstems in the same region accidental urban wetlands studied in ew erse and Ariona A appear to support redo conditions that facilitate high rates of microbial denitrification within a few decades following formation Accidental wetlands in demonstrated comparable or higher rates of denitrification than remnant wetlands surrounded b urban development or native wetland sstems and these rates often matched or eceeded nitrate ‐ loading from stormwater and the atmosphere Denitrification rates in these wetlands were mediated b ‐ ‐ soil pore structure and water dnamics and limited b availabilit n A percent removal of b accidental wetlands was generall high and matched or eceeded removal in native or constructed wetlands in ‐ the same region The wetlands were often a net source of dissolved but on average removed 1– of entering the wetlands These high levels of function suggest that structural elements soil structure hdroperiod mediating beneficial microbial processes in urban wetlands ma in some cases develop relativel uicl in the absence of design and management

Dr alta is an Assistant rofessor of nvironmental cience and a denitrification enthusiast he has spent the last 0 ears of her life mostl in waders eploring and studing wetlands in the outheastern ortheastern and outhwestern he is currentl involved in proects eamining urban wetlands in ew or it

ontact nformation onica alta Department of nvironmental tudies and cience 1 ar ow m 1D ew or A 11

134 April 23-26, 2018 | Coral Springs, Florida, USA

LARE ETANE EIIN R AAN LDLAIN TREE , ad Baten, dward Hornroo, e nrhrat, nent au ancaster nvironment entre ancaster niversit ancaster eartment of hematic tudies nvironmental hange ining niversit ining weden eartment of arth nvironmental and eograhic ciences he niversit of ritish olumbia ritish olumbia anada chool of nvironment arth and cosstems he en niversit ilton enes

etlands release methane a owerful greenhouse gas to the atmoshere via three main athwas diffusion bubbling and lantmediated transort owever there is growing evidence that emissions ma also occur via stem and leaf surfaces of wetland trees ur earlier wor in temerate troical and boreal forested wetlands revealed that trees emit ≤90% of CH in forested wetlands hese findings together with man other recent studies have highlighted the gross underestimation of emissions from wetlands b ecluding measurements from trees

ere we resent our recent stem emission measurements from over trees across locations in the central railian Amaon basin e found tree stems to emit – mg m− h− which are about times larger than emissions reorted for temerate wet forests and troical eatswam forests maing them the largest nonebullitive wetland flues recorded tem emissions eceeded emissions from leaf soil auatic and macrohte surfaces ess than of wood cores etracted from tree stems at and cm above the soil or water surface dislaed caacit for roduction which is indicative that the emissions measured at the stem surfaces are rinciall of soilmicrobial origin he  values of stem emissions ranged from to 59.1‰, averaging 66.2 ± 6.4‰, these values are typical for wetland soil CH

sing measured flues and their corresonding surface area we estimated the contribution of each transort athwa to total ecosstem flu missions from tree leaves and stems collectivel were the dominant source of evasion from Amaon floodlain soil onservative scaling the stem and leave emission data to the entire Amaon lowland basin ields an annual source strength of u to g r for tree mediated emissions ur findings from the Amaon basin highlight that treemediated emissions reresent a sieable source of to the regional budget and illustrates the imortance of considering treemediated emissions in large scale assessments and rocessbased wetland models

r angala is a esearch fellow and lecturer at ancaster niversit with research interests in lantsoil interactions and greenhouse gas echanges from carbondense forests ornean eatswam forests and railian Amaon feature heavil in her wor and in these ecosstems she has been studing the role of trees in transorting methane to the atmoshere

ontact nformation ancaster nvironment entre ancaster niversit ailrigg ancaster A

135 12th International Symposium on Biogeochemistry of Wetlands

AE WE REACED A NEW NRAL NUTRIENT CCLIN AND BL DNAIC IN TE INDIAN RIER LAN , dward h, araret a and atr nett

P) as the driver of blooms in coastal systems. We investigated potential ‘bottomup’ factors— — up to a “brown tide” event in 2015

Together, these internal nutrient cycling processes may help lead to a ‘new’ ecosystem state favoring widespre

136 April 23-26, 2018 | Coral Springs, Florida, USA

NUTRIENT DNAIC AND TEIR INTERACTIN WIT TLANTN RWT IN TE AUATIC AREA CATAL WETLAND IN LIAE DELTA CINA ,, dward aw and uan e, 1ey aboratory of oastal Wetland iogeosciences, hina eological urvey, 201 ingdao, hina 2aboratory for arine eology, ational ceanography aboratory, 201 ingdao, hina epartment of nvironmental ciences, chool of the oast and nvironment, ouisiana tate niversity, aton ouge, ,

luvial inputs of nutrients and efficient nutrient recycling mechanisms mae estuarine and coastal waters of wetlands highly productive. or the same reasons, they are susceptible to eutrophication problems. n hina, eutrophication problems along coastal wetlands are becoming serious because of discharges of domestic sewage and industrial wastewater and runoff of agricultural fertilier. ddressing these problems reuires an informed assessment of the factors that controlling wetland algal production. ur study aims at determining whether nutrients were limiting to phytoplanton growth, their interactions, the possible factors controlling phytoplanton biomass, and the effect of nutrient variations on the trophic status in the auatic areas of coastal wetland in iaohe elta, hina. ased on an investigation during the autumn of 201, we found no evidence of nutrient limitation for phytoplanton growth in our study. mmonium concentrations were high enough to suppress nitrate uptae, and therefore ammonium was the principal source of nitrogen for phytoplanton in the surface waters. itrate and silicate followed a conservative miing pattern versus salinity. The maor factors limiting phytoplanton growth in this study were light availability and low temperature. The degree of light limitation was most etreme in the iaohe iver mouth but weaer in offshore areas. This fact, combined with nutrient over enrichment stress, has caused serious eutrophication problems and freuent occurrences of dinoflagellate blooms in the auatic area of coastal wetlands of iaohe elta.

r. Pei is an associate research scientist from the ey aboratory of oastal Wetlands, hina eological urvey, with etensive interests in biogeochemistry, nutrient pollution and eutrophication problems of coastal wetlands. e is awarded the istinguished oung cientists Training Program in inistry of and and esources and has led 5 proects.

ontact nformation haofeng Pei, ey aboratory of oastal Wetlands, hina eological urvey, o.2, uhou oad, ingdao, hina, Post code 201

137 12th International Symposium on Biogeochemistry of Wetlands

W D WE ANAE ICRBIE T RTE URBAN WETLAND UNCTIN , ena Bedoe, and an Bean 1ast arolina niversity, reenville, , 2niversity of lorida, ainesville, ,

utrient runoff from urban development has led to elevated contaminant inputs into auatic ecosystems. To mitigate nutrient runoff, best management practices, such as stormwater wetlands are incorporated into urban landscapes to enhance flood control and improve water uality. owever, increased environmental stressors, such as drought and salinity can affect microbial communities, thus threatening wetland nitrogen removal functions. n most engineered and restored ecosystems, the contribution of microbial communities is often ignored even those these organisms determine the types and rates of ecosystem functions. To eamine how environmental features of urban wetlands influence microbial community structure and function, we ran potential denitrification assays acetylene bloc method) to uantify nitrogen removal rates and characteried bacterial community composition amplicon seuencing method) along riparian areas of an urbanied stream and constructed stormwater wetland. istinct bacterial communities were associated with the highest sediment denitrification rates in shady, vegetated, and wetter areas, compared to denitrification rates in unvegetated wetland sediment and base and stormflow water samples. To uantify the impacts of environmental stressors on nitrogen removal potential, we ran a laboratorybased manipulation of moisture and salinity on constructed stormwater wetland sediments. esults revealed that salinity significantly reduced denitrification rates under the most saturated moisture conditions. ifferences in potential denitrification rates are due in part to changes in bacterial community responses to environmental stressors such as moisture and salinity manipulation and organic carbon inputs. To promote the design and management of resilient constructed stormwater wetlands, we need to now how environmental change interacts with the built environment if we are to support urban wetland functions.

r. Peralta is an ssistant Professor of icrobial cology. er research eamines how human changes to the landscape impacts microbial community structure and ecosystem functions. ecent proects leverage socialecologicaltechnological approaches for targeting restoration of microbial functions in managed ecosystems.

ontact nformation riane . Peralta, epartment of iology, ast arolina niversity, 01 owell cience omple, ail top 551, reenville, , 25

138 April 23-26, 2018 | Coral Springs, Florida, USA

REBUILDIN IIII RIER DELTA ERATIN A EDIENT DIERIN T BALANCE ECTE AND CIAL NEED , e ae, ae owan r, urao ut, e Koer, hre aa, e oruodae, ar eanon r, ohn an, oert we, ennee er, ohn hte, and ae n 1nvironmental efense und, Washington , 2ouisiana tate niversity, aton ouge, , ouisiana niversities arine onsortium, hauvin, , niversity of ew rleans, ew rleans, , 5icholls tate niversity, Thibodau, , niversity of ouisiana at afayette, afayette,

Louisiana’s coastal future relies on reconnecting the Mississippi River to the delta and allowing the water, sediment and nutrients from the river to nourish and sustain eisting marshes and rebuild lost wetlands. Through a series of gates, a restoration infrastructure proect called a sediment diversion can allow discharge of up to 5,000 cfs from the river to the adacent marshes at various times and flows. The very nature of the sie of the flow, similar at times to the average flow of the issouri iver, can fundamentally alter the ecological and social landscape. The inherent fleibility of the structure and its operations reuire a dynamic balancing of multiple obectives and constraints of the ecosystem and the communities that rely on those resources.

To access these interconnected relationships, we formed an interdisciplinary woring group of eperts to eplore, discuss, debate and document the state of the nowledge, data gaps, trigger points, monitoring and operational strategies. ver an eightmonth period, the woring group, along with 2 other invited guest eperts, evaluated how the operation of a sediment diversion could affect every maor aspect of the coast. This was accomplished by maimiing operations for each single obective, void of other constraints, and then evaluating the commonalities and dissimilarities in each strategy.

or instance, biogeochemistry plays an important role in the health of coastal wetlands, including nutrient loading, sulfates and sulfides, soil conditions, toins and carbon. The woring group evaluated uestions such as “how could operations maximize denitrification?” to develop operation strategies, including flow capacities, length of operations and seasonality. The woring group also evaluated ey thresholds that could lead to induced harm and monitoring needs to adaptively manage the diversion. This activity was repeated for each ey aspect of the landscape, including river and basin hydrodynamics, geomorphology and sediments, water uality, wetland health, fish and wildlife species, and communities and socioeconomics. The woring group also evaluated governance and staeholder issues.

The recently published results included a set of recommendations for operational strategies, both initially and over longterm operation of the diversion. The woring group is currently testing the recommendations through hydrodynamic, morphology and vegetation models to determine how the epert predictions of outcomes compare to the predictions of , processbased models. This presentation will summarie the interdisciplinary approach to an environmentally and socially comple problem, overview the recommendations of the woring group and provide some preliminary modeling results from the ongoing effort.

atalie is the irector of cience Policy at . Previously, she served as the technical and outreach lead for the 2012 oastal aster Plan. he has a .. in Wildlife and isheries, a .. in ceanography and oastal ciences and is a Ph candidate in arinestuarine nvironmental cience.

ontact nformation atalie Peyronnin, nvironmental efense und, 15 onnecticut venue, uite 00, Washington, , 2000

139 12th International Symposium on Biogeochemistry of Wetlands

REEALUATIN TE CNEUENCE LAND UE ACCELERATED DILUTIN ELIC ATE DEITIN IN UIC LAE , odd orne,, e Harr, neue K Bohna hitne Laorator for Marine iosciences, niversit of lorida, t ugustine, L, etland iogeochemistr Laorator, oil and ater ciences epartment, niversit of lorida, ainesville, L, oil Mineralog Laorator, oil and ater ciences epartment, niversit of lorida, ainesville, L, ater Resources epartment, nvironmental onsulting and echnolog, nc, ainesville, L,

ince the estalishment of the lean ater ct, managers have sought to reduce pollutant loads to impaired laes to reach total maximum dail loads owever, nonpoint source pollution continues to challenge pollutant management ne potential source of pollution is erosion and sediment transport from uran areas with large amounts of concentrated runoff hile erosion and sediment transport is not tpicall a maor pollutant for nutrients, Florida’s geology indicates a closer look. The U.S. Geological urve suggests that geological units with notale amounts of phosphate can e found at or near land surface across of lorida his estimate does not tae into account further exposure erosion, channelization, and earth moving activities herefore, erosion and sediment transport in this geologic setting ma e a significant source of phosphorus loading to drainage laes urthermore, changes in the intensit of storm events are liel to further exacerate this issue n this stud, we examined the factors that dictate geologic phosphate dissolution in eutrophic humic laes

eologic phosphate is often considered stale and relativel innocuous despite high total phosphorus concentrations owever, in humic drainage laes, iogeochemical conditions ma create an environment conducive to accelerated geologic phosphate dissolution, there increasing concentrations of iologicall availale phosphorus ur findings from dissolution experiments in several humic laes underlain the phosphaterich awthorn roup help to understand whether erosion and sediment transport of geologic phosphorus is meaningful to phosphorus loads in such laes hese findings ma indicate a new approach to phosphorus management, load estimates, and prioritization for humic drainage laes found in this geologic setting urthermore, we suggest risassessment techniues for certain land uses and associated activities to prevent new geologic phosphate exposure and transport in vulnerale watersheds

ara helps is a research fellow in the oil and ater ciences epartment etland iogeochemistr La at the hitne Laorator for Marine iosciences he has over ears of experience in environmental consulting er research focuses on land use impacts on soil weathering processes, auatic iogeochemistr, and water ualit

ontact nformation ara helps, oil and ater ciences epartment, Mcart all , ainesville, L,

140 April 23-26, 2018 | Coral Springs, Florida, USA

NEL INTERACTIN A AECT RANE EANIN I EA UNULATE BRWIN RETRAININ ANRE ADANCE N TE UT TEA CAT , eandro erroao, a eer Uniersity of Teas io Grande alley, ronsille, T, US Sithsonian nironental esearch enter, dgeater, , US

s gloal teperatures continue to increase, arious tropical and sutropical species are predicted to epand their range. ne ell kno case inoles the angroes oeent poleard, replacing saltarsh ecosystes. angroes are iportant for coastal systes ecause they proide haitat for any organiss, itigate erosion, and seuester caron and nutrients. s the angroes inade saltarsh systes, shifts in the oerall ecosyste structure and function occur and noel iotic interactions are osered.

long the Gulf of eico, at the southern tip of Teas, recent oserations hae shon that epanding lack angroe enna ernan stands are eing rosed intensiely on y inasie nilgai antelope Boeahu traoaeu and less freuently y natie hite tail deer dooeu rnanu. The antelope ere introduce to the south Texas region via the King Ranch in the 1930’s and have since significantly increased in population and distriution. Generally thought to e graers, B traoaeu hae een recurrently recorded on caera rosing on ernan. reliinary caron and nitrogen stale isotope analysis suggest that B traoaeu prefer angroes and other oody species oer the aundant, higher protein content grasses. This interaction has not een docuented and appears to arkedly alter angroe plant architecture, etaolis and reproductie output.

n order to assess the effects of heay rosing y B traoaeu, selected structural and functional characteristics of ernan ere copared eteen rosed and nonrosed trees. s epected, rosed trees hae a saller canopy and reduced eaf rea nde, ut also ehiit a change in resource allocation and hae loer resorption ratios of nitrogen and phosphorus. rosed trees tend to hae leaes ith higher content aking the a higher uality forage, and perhaps prooting recurrent rosing on affected trees. eaf chlorophyll content in rosed trees as loer, possily pointing at ore stressed or less healthy plants. rosing negatiely affected the aount of inflorescences and produced saller propagules. erterate heriory on ernan in south Teas is considered a noel interaction, and ay ell constitute a negatie feedack to the range epansion of this estuarine foundation species.

Tianna icuet is a graduate student at the Uniersity of Teas io Grande alley, pursuing her S in iology. er current research focuses on plantanial interactions ithin coastal etlands. She also has a S in iology fro leeland State Uniersity here she focused on iodiersity sureys ithin forests and etlands.

ontact nforation Tianna icuet, Uniersity of Teas io Grande alley, ne est Uniersity ld, ronsille, T US

141 12th International Symposium on Biogeochemistry of Wetlands

ICRBIAL ENE ACTIIT IN A TRWATER TREATENT AREA IN RENE T INLW LW CNDITIN outh lorida ater anageent istrict est al each

The verglades torater Treatent reas Ts have een constructed to reduce phosphorus fro the ater colun as a aor coponent of the verglades Restoration efforts The Ts are reuired to produce ultralo concentrations at the outflos to protect the receiving ater odies s part of a largescale scientific research to understand the internal processes ithin the T that produce these desired outflo concentrations the role of the icroial counities in nutrient cycling ithin the T is eing exained ince in the outflo ater in ellperforing Ts are priarily in particulate and dissolved organic fors nutrient cycling y the icroial counity provides critical pathays to readon of these fors of The level of enye activity is often used as an indicator of nutrient liitation of the syste or to evaluate shifts in the nutrient liitation ie fro liited to nitrogen liited The agnitude and distriution of enyes associated ith the release of and and the readon of caron copounds ay differ along the nutrient gradient and in response to flo conditions

urrently there is liited data availale regarding the influence of flos on the rates of exoenye activity in the Ts and the agnitude of icroial influence on cycling in the Ts This presentation is focused on enye activity easureents in T loay 3 a 3acre freshater etland located in southern lorida that is doinated y suerged auatic vegetation and has een operational since 1999 The enye activity easureents in the surface ater and periphyton counities as the floay is suected to variale flo conditions is discussed The enyes assayed are those associated ith acuisition alaline phosphatase phosphodiesterase caron acuisition glucosidase and acuisition enye leucine ainopeptidase apling as conducted prior to and during the flo events and during stagnant conditions

or periphyton higher activity as oserved during stagnant conditions than under flo conditions for all the enyes onversely there as greater enye activity easured in the surface ater during flo conditions n the periphyton, the activity of phosphatase, phosphodiesterase, and Βglucosidase increased along the nutrient gradient fro inflo to outflo hile the activity of leucine ainopeptidase as coparale aong all locations o consistent gradient in enye activities as oserved in the surface ater or oth the surface ater and periphyton enyatic activity as loest in the uer copared to the pring and all sapling events

Kathleen ietro is a senior environental scientist ith over 30 years of experience oring ith southern lorida freshater etlands er expertise is in evaluating the nutrient reoval perforance of the verglades torater Treatent reas and her current research is focused on the influence of the icroial counities ithin the etlands

ontact nforation Kathleen ietro outh lorida ater anageent istrict 3301 un lu Road est al each 330

142 April 23-26, 2018 | Coral Springs, Florida, USA

LANTEDIATED ETANE AND CAN ECANE IN A BREAL ULAND RET ,, uho ato, Hannu rte, a Haaranen,, a Haeen,, u Henonao,, Katerna ahaoa, etoa, nuna utnen,, ar ann and nna antaaht, 1ational nstitute for ntegrated tospheric and arth yste cience Research R niversity of elsini inland epartent of gricultural ciences oil and nvironental ciences niversity of elsini inland 3yytil forestry field station niversity of elsini inland atural Resources nstitute inland elsini inland epartent of orest cience niversity of elsini inland innish eteorological nstitute elsini inland loal hange Research nstitute rno ech Repulic epartent of ood and nvironental cience niversity of elsini inland

orest soils are generally considered as a sin of atospheric ethane hile trees and vegetation are not accounted in the alance of forests Recent studies sho that trees ay oth eit and consue fro the ste and fro the canopy and y doing so significantly influence the alance of forest ecosystes ur ai is to partition fluxes in oreal forests to different coponents forest floor ground vegetation and trees and to evaluate the role of trees in the alance of oreal forests

e have easured fluxes at forest floor tree ste and tree canopy levels y enclosure ethods during 01 01 in a oreal forest at yytil southern inland R station e have also easured fluxes aove the forest canopy using flux gradient and eddy covariance ethods conducted laoratory experients to partition fluxes eteen elo and aoveground parts of forest vegetation and studied the capacity of tree stes to transport in controlled laoratory conditions dditionally e have assessed the role of ethane oxidiing and ethane producing icroes in different coponents of the forest oining these easureents allo us to evaluate the roles of soil and trees as sources and sins of in the forest and assess the contriution of trees to the alance of oreal forests

e found that trees eit fro their ste and fro the canopy Treeste eissions have strong seasonality and the fluxes differ aredly eteen tree species and eteen groing haitats The leaflevel canopy exchange involves oth eission and consuption processes hile the drivers of the exchange are still poorly understood ased on our ultiyear easureents it is clear that oreal trees are a significant source of and e propose that trees should e accounted hen assessing the udgets of forests e also recognie that our understanding of the exchange processes is still incoplete and to solve that e need ore process studies coined ith stateoftheart field easureents

ssociate rofessor ari ihlatie is a group leader and a of soil research at R R ecosyste stations in inland he is an expert in plantsoil interactions and ethane and nitrous oxide exchange processes in forest ecosystes he as recently aarded a prestigious uropean Research ouncil R starting grant

ontact nforation ari ihlatie epartent of gricultural ciences ivision of oil and nvironental ciences atoartanonaari 9 0001 niversity of elsini inland

143 12th International Symposium on Biogeochemistry of Wetlands

LECULAR AND ATICLEEL ARACE T CARACTERIE DILED RANIC ATTER INIT R ERCUR BIAAILABILIT IN TE LRIDA EERLADE , ara Breteer, atthew one, oeh an, a re, ad Kraenhot 1 eological urvey oulder niversity of olorado oulder oulder 3 eological urvey Reston eological urvey iddleton

issolved organic atter in the lorida verglades controls a nuer of environental processes iportant for ecosyste function including light asorption ineral dissolution and precipitation and the transport and reactivity of etals ercury g speciation and ioavailaility in the lorida verglades in particular are intiately lined to sulfur cycling and interactions ith nder sulfate reducing conditions hich develop in response to agricultural sulfate inputs to verglades etlands sulfide reacts ith to for highly reduced sulfur species ie thiols in the these highly reduced sulfur groups strongly coplex divalent ercury and increase the ioavailaility of ercury in the for of nanocolloidal ercuric sulfide Recent advanceents in the understanding of cheistry on ercury cycling in the verglades has relied on the application of ne tools that provide atoic and olecularlevel inforation

This presentation ill highlight the application of eerging tools in the characteriation of including 1 atoiclevel characteriation of organic sulfur y ray asorption spectroscopy and stale isotope easureents and olecularlevel characteriation of y ultrahigh resolution ass spectroetry The aove entioned approaches ere used to characterie isolated fro surface and pore aters at a variety of locations in the lorida verglades over several field capaigns fro 0101 ray asorption spectroscopy hich uantifies the relative aundance of different organic sulfur functionalities as used to deonstrate a spatial dependence in sulfur content and speciation in verglades etlands that results fro sulfuriation of in sulfidic sedient pore aters ltrahigh resolution ass spectroetry hich uantifies olecular signatures of uniue containing olecules provided evidence that enriched in reduced sulfur in sedient pore aters exchanges ith in overlying surface aters The staility of reduced sulfur in verglades to oxidation as elucidated using oth ray asorption spectroscopy and ultrahigh resolution ass spectroetry on i field saples collected fro surface aters exposed to natural oxidation and ii laoratory saples suect to controlled light and dar oxidation astly e present results on the use of stale sulfur isotopes to differentiate eteen different sources of sulfur in verglades eg sulfate fro atospheric sources versus agricultural inputs nforation gained here on sulfur cheistry provides a fraeor to evaluate sulfur dynaics in response to teporal fluctuations in sulfate loading and uild on previous ors that define the factors influencing ercury ethylation in the verglades ecosyste

r oulin is a cheist at the eological urvey in oulder olorado is research focuses on understanding ho the cheical nature of dissolved organic atter influences the environental cycling of aor eleents eg caron sulfur and trace etal containants eg ercury

ontact nforation rett oulin ydrocological nteractions ranch eological urvey 31 arine t T 1 oulder 0303

144 April 23-26, 2018 | Coral Springs, Florida, USA

CALLENE CNNECTIIT WITIN URBAN LANDCAE EALE R TE BALTIRE ECTE TUD , a o, rnn hond, ua Kauha, and eter roan, 1oil and ater ciences epartent niversity of lorida ainesville ary nstitute of cosyste tudies illroo 3ater tudies entre chool of heistry onash niversity layton ictoria epartent of eology arth yste cience nterdisciplinary enter niversity of aryland ollege ar ity niversity of e or dvanced cience Research enter at the raduate enter and roolyn ollege epartent of arth and nvironental ciences e or

onnectivity of uran ater odies has een sustantially changed due to hydrological physicocheical and iological alterations throughout upland riparian and auatic environents or exaple drainage netors of ipervious surface areas often use stor drains to allo runoff to ypass infiltration or riparian ones causing flashier hydrographs and increases in containant loading n addition to direct effects on ater odies uraniation can also alter reciprocal connections fro auatic to terrestrial environents or exaple direct connections eteen asteater and uran ater odies causes increased pharaceutical loading in uran streas hile pharaceuticals have yriad direct effects on ecosyste structure and function ithin strea ecosystes they also ipact the eergence of auatic acroinverterates reducing the connection eteen auatic and terrestrial ecosystes n addition various strategies for rehailitating uran ecosyste functioning eg retention ponds floodplain restorations are often aied at enhancing connectivity either explicitly or iplicitly sing results fro the altiore cosyste tudy and other or focused on coon uran containants e ill suarie the ipacts of connectivity ipairents in uran auatic ecosystes n particular e ill focus on ho nuerous proles associated ith uraniation are driven priarily y altered connectivity and ho these proles ay e addressed y directly iproving connectivity issues across the uran landscape

r Reisinger is an assistant professor of uran soil and ater uality focusing on nutrient and energy dynaics of uran ecosystes e recently oved to lorida ut previous or has focused on uran strea restorations in altiore as ell as nutrient cycling in large rivers throughout the

ontact nforation lexander Reisinger oil and ater ciences epartent niversity of lorida 11 carty all ainesville 311

145 12th International Symposium on Biogeochemistry of Wetlands

ECLICAL CNEUENCE INAIE LANT ECIE INTRDUCTIN T LCANIC LAE IN CENTRAL AERICA AN EALE LAE ATITLAN UATEALA Eliška Rejmánková, ene nder, tehane ate 1niversity of alifornia avis rsuline ollege leveland

ifferent functional groups of acrophytes vary in their ipact on auatic ecosyste structure and processes Replaceent of one functional group y another often resulting fro an introduction of an invasive species can have serious and often irreversile conseuences

e ill docuent and explain physicocheical and etaolic changes in littoral ones of ae titln uateala folloing invasion y floatingleaved and suersed acrophytes hhorna rae and Hdra ertata and the replaceent of native eergent species hoenoetu aornu e utilied oservational data fro efore and after invasion of Hdra plant ioass and ater characteristics data sapled along transects through littoral ones and gas eission easureents in controlled esocoss and in the lae

While the three species don’t differ significantly in their standing crop biomass, there is a major difference in the depth distriution of Hdra and hoenoetu ith Hdra having axiu ioass in ater depths of hile hoenoetu reaches axiu ioass in aters 0 deep The to invaders have higher turnover rates than hoenoetu ignificant differences in nutrient coposition of the three species ith hhorna and Hdra containing less caron and thus exhiiting loer and ratios than hoenoetu lead to their faster decoposition rates and potential shift in nutrient cycling ithin the ecosyste

ater oxygen profiles differ aong the three species hoenoetu stands aintain a ell oxygenated ater colun issolved oxygen ithin Hdra stands is supersaturated in the upper 100 c roughly coinciding ith the densest part of upper canopy decreases ith depth ut does not drop elo 0 issolved oxygen in hhorna doinated areas is characteried y 00 saturation and it drops to aout 30 near the otto oth hoenoetu and hhorna patches exhiit coparale fluxes seuestering 30 and 300 g 1 1 h respectively during the day and eitting 0 and 00 g h respectively during the night ontrary to 1 these to species Hdra is seuestering during the day 3 g h as ell as at night g h1

ur description of changes to the littoral ecosyste in ae titln is representative of other entral erican volcanic laes exposed to rapid eutrophication and spread of invasive species

r Renov is a professor of auatic and etland ecology in the epartent of nvironental cience and olicy at avis ith research focus on structure and functions of etland ecosystes in entral erica

ontact nforation Eliška Rejmánov epartent of nvironental cience and olicy niversity of alifornia avis avis 91

146 April 23-26, 2018 | Coral Springs, Florida, USA

CEICAL CNTRL N CARBN EUETRATIN AND LU ALN A BREAL T TRICAL RADIENT i Riaon, anaan, H an , Ho, Hodn, nton, B ooer, and hanton ke niersity Wetland tr, icholas chool of the Enironment, rham, , epartment of hemistry and iogeochemistry, lorida tate niersity, allahassee, , epartment of Earth, cean and tmospheric cience, lorida tate niersity, allahassee, ,

iogeochemical controls reglating carbon storage and gas fl in peatlands are nderestimated compared to hydrologic and anaerobic reglators o eamine the role of carbon ality in accretion in northern compared to tropical peatlands e completed field and lab stdies on bog peats collected in innesota, orth arolina, lorida and er to anser three fndamental estions is tropical peat more recalcitrant than northern peat does the addition of aromatic and phenolic componds increase toards the tropics do differences in the chemical strctre of organic matter eplain ariances in carbon storage and fl in tropical erss northern peatlands r main hypothesie is that high concentrations of phenolics and aromatic componds prodced in shrb and tree plant commnities in peatlands copled ith the fire prodction of biochar aromatics in peatlands may proide a dal biogeochemical latch mechanism controlling microbial decomposition of peat een nder higher temperatres and seasonal droght y comparing the peat bog soil cores collected from the peat bogs, ocosins, Eerglades and er palm samps e find that the soils in the shrbdominant ocosin contain the highest phenolics, hich microbial stdies indicate hae the strongest resistance to microbial decomposition and a distinctie dominant fngal commnity chemical comparison of plant drien peat carbon ality along a north to soth latitdinal gradient indicates that tropical bog peatlands often hae higher aromatic componds, and enhanced phenolics, especially after light fires, hich enhances storage and affect fl

rofessor rtis Richardson is a Wetland Ecologist and irector of the ke Wetland enter e has orked on biogeochemical cycles of , and in freshater etlands for more than years e has pblished more than peerreieed papers in top jornals, inclding, cience and atre as ell as books on the Eerglades and the ocosins

ontact nformation rtis Richardson, ke niersity Wetland enter, icholas chool of the Enironment, o , ke niersity, rham, ,

147 12th International Symposium on Biogeochemistry of Wetlands

CANE IN CARBN AND NITREN IN A DRAINED UBTRICAL WETLAND TE CAE TE EERLADE ARICULTURAL AREA ne Roie, ara arou, and tean erer Eerglades Research and Edcation enter, nstitte of ood and gricltral ciences, niersity of lorida, elle lade, , oil and Water ciences epartment, niersity of lorida, ainesille, ,

rainage of the Eerglades gricltral rea E soils has reslted in soil sbsidence bsidence rates hae been redced compared to preiosly reported rates ncreases in mineral content, recalcitrant carbon, and higher ater tables attribted to best management practices, are considered possible cases for this redction he objecties of this research are to determine ho soil lability and ater management affect sbsidence rates, and ho and pools and fles hae changed as a reslt of the sbsidence process o establish this, oidation rates effl of shallo and deep E soils eposed to for ater management treatments ere ealated along ith measrements of , , solble organic nitrogen , and dissoled organic carbon performed on leachate dditionally, a fractionation procedre is being implemented to determine the sie of the labile and recalcitrant pools in these soils reliminary reslts indicate that soils eposed to days flooding – days draining cycles dring the smmer season hae the highest effl rates mg of m h he in leachates is highest in flooded soils to mg compared to drained soils, hereas is highest in drained soils ith concentrations as high as mg imilarly, the and hae higher concentrations in drained soils, in the case of , deep soils hae higher concentrations mg compared to shallo soils mg rganic matter and the hot ater etracted the most labile pool are higher in deep soils compared to shallo soils ll pools decrease at deeper increments in the soil profile he ratio in deep soils is higher compared to shallo soils reliminary reslts indicate that ater management is of great importance controlling and cycling in sbsiding histosols Reslts also sho that the distribtion of soil pools aries ith depth in these soils, hoeer these differences in fractions do not case differences in oidation rates

ndres Rodrige is a h candidate in the oil and Water ciences epartment at the niersity of lorida e has a c degree in enironmental stdies from lorida nternational niersity and a c in iology from the niersidad acional de olombia

ontact nformation ndres Rodrige niersity of lorida , oil and Water ciences epartment, carty all , o , ainesille,

148 April 23-26, 2018 | Coral Springs, Florida, USA

TE RLE CANBACTERIA IN NUCLEATIN TE RECIITATIN CALCIU CARBNATE IN TE EERLADE ATERITE AND ARANITE a Roen

tonea hothr aoa tonea hothr

tonea hothr tonea hothr hothr

149 12th International Symposium on Biogeochemistry of Wetlands

LBAL CNTRL CARBN TRAE IN ANRE IL ne Rovai,, oert we, dward ataedaoa, ao u, ue uenteara, arn anrowaoo, auo Horta,, o ona, eandra onea, auo aoa,

54% of the world’s carbonate settings occur in the neotropics,

150 April 23-26, 2018 | Coral Springs, Florida, USA

LEN R LANDCAE BUDET IN TE DEC ICAL DEL AND AR LU EERLADE NATINAL AR EN olin ane, ue ewan, r ateBodt, udo a, eter eer, Barr H oen, ud Hare, r ne, hrta we, a ho, hae anna, hr Hanen,, red ar outh lorida ater anageent istrict, est al each, , lorida nternational niersit, iai, , niersit of alifornia at erele, erele, , 4 eological ure, rlando, , 5 eological ure, eston, ,

critical coponent of future erglades restoration proects includes the restoration of sheetflow he ecop hsical odel , a landscapescale 5 field test, recentl deonstrated restored flows enhance sedient transport a e echanis to restore ridgeandslough topograph, confiring largescale odel predictions n addition, the , reealed biological responses that feedbac on flow, not anticipated b odels n three flow eents, floating periphton was cleared fro sloughs eperiencing high elocities 5 cs, an iportant echanis in which reduced egetatie resistance led to increased elocities with flow duration onger flow durations also corresponded with changes in floc source greater greenalgal deried and increased floc erodibilit e hpothesie these biological responses to flow a be as or ore iportant than phsical echaniss regulating sedients in sloughs and ridges e address this hpothesis b using an ecosste ass balance odel to identif the priar echaniss controlling stocs and flues of sedient and hosphorus under high and low flows he odel originall deeloped for har lough, erglades ational ar, was calibrated for the stud area and siulated for ultiple scenarios, under different cobinations of biological and phsical responses to flow coparing odel scenarios against obserations, our preliinar findings indicate that incorporating phsical and biologicallediated flow effects enabled the odel to fit obsered changes in water colun and suspended sedients, sedient transport, and floc standing stocs of howeer, additional flowediated echaniss were reuired he ost surprising obseration was that while floc olue, ass and carbon decreased under high flow, floc increased b 4% oer baseline lowediated echaniss reuired to fit these obserations include increasing auatic assiilation rate b slough egetation liel drien b increased bioass total and adding sedient capture and release postflow in suberged auatic egetation hile reduced floc carbon and ass stocs support the echanis of sedient redistribution in restoring ridgeslough topograph, concoitant increases in floc stocs, and organic atter ualit eg, as food for consuers a ipact longerter changes in priar and secondar production in sloughs, all of which a reuire seeral ore ears to full deelop ais, 4 ewan et al, lthough these changes were clearl eident in areas receiing highflows, challenges reain in restoring large areas, as high flows and increased sedient obiliation were liited in scope to 5 radius fro the control structure

eferences ais, , 4 hosphorus nputs and egetation ensitiit in the erglades, in ais, , gden, ds, erglades he cosste and its estoration, t ucie ress, elra each, pp 5

ewan et al, ewan, , chuette, , race, , utche, , ontaine, , edd, , ietrucha, , actors nfluencing attail bundance in the orthern erglades, uatic otan , 5

r aunders is a ead cientist in the erglades stes ssessent section of the and currentl seres as tea lead of the interagenc science tea for the ecop hsical odel, a field test ealuating sheetflow benefits in ater onseration rea

ontact nforation olin aunders, erglades stes ssessent ection, outh lorida ater anageent istrict, 4 eledere oad, est al each, , 4

151 12th International Symposium on Biogeochemistry of Wetlands

ETANENIC ARCAEA DINATE ATURE EE EARTWD ABITAT ane , on eah, an an, ea reer and ioe a iosciences iision, a idge ational aborator, a idge, , epartent of icrobiolog, niersit of ennessee, noille, , ual ontribution

ethane is a potent and ipactful greenhouse gas which has accuulated in the lower atosphere and accounts for % of radiatie forcing ecentl there hae been widespread reports fro dierse forest tpes deonstrating that the direct eission of ethane fro the truns of liing trees a be a significant source of terrestrial eissions ontrastingl, reports of ethanogens fro wood of ouu tree species were proinent in the literature 4 ears ago, but hae not been reisited with odern olecular icrobial ecolog approaches e eained the icrobial counities associated with the heartwood and sapwood of astern ottonwood ouu detode trees in ennessee using lluina r gene aplicon approaches t sapling trees often eitted flaable gasses, and analsis results showed both enironents were priaril coposed of icrobial taa associated with anaerobic enironents or eaple, ethanogens were proinent % oerall in heartwood enironents, aing up on aerage 4% relatie abundance, ersus % in sapwood enironents and priaril classified as acetoclastic ethanoateru spp ebers of the iricutes phlu oerall were % of seuences and in 4% greater abundance in heartwood oer sapwood enironents easures of wood oisture content, p of the wood, and tree were also significant predictors of taon abundance and oerall coposition patterns ur results show that unlie ore well studied plant associated counities in the soil, roots, rhiosphere and phlosphere, wood associated counities are shape b the uniue enironental conditions of the habitat and a be proinent and oerlooed sources of ethane eissions in teperate forest sstes

ontact nforation hristopher chadt, a idge ational aborator, a idge, ,

152 April 23-26, 2018 | Coral Springs, Florida, USA

EECT URRICANE IRA N DILED RANIC CARBN LUE ALN A ALINIT RADIENT ae ae , ard , au uan , K edd , and odd orne , niersit of lorida, oil and ater ciences epartent, ainesille, , niersit of lorida, hitne aborator for arine iosciences, t ugustine, , arine ciences aborator, eui, ,

isturbance eents, such as hurricanes, create copious aounts of runoff fro terrestrial uplands that flow into streas and riers and eentuall out into the ocean long with the runoff, dissoled organic atter also runoff the landscape and into these waterwas where the a react and undergo further transforation issoled organic carbon is one of the ost prolific and iportant coponents of as a whole o deterine how concentrations of were affected b urricane ra in t ugustine, lorida, water saples were collected at four sites along the gradient fro upstrea ellicer ree off of 4 to the doc behind the hitne aborator for arine ioscience near the ocean inlet oer a period of onths issoled organic carbon concentrations ranged fro alost g to 5 g at hitne oc and 45 g to 5 g at 4 concentrations increased at hitne oc during urricane ra and continued to increase for approiatel a wee after the stor hit oweer, sapling at 4 showed where concentrations increased before and as the hurricane hit and then decreased as it was flushed out of the area and towards the coast concentrations too onths to finall stabilie bac to prehurricane conditions

s chafer is a h student in the soil and water sciences departent at the niersit of lorida adised b r edd and r odd sborne he has been studing estuarine science for the past four ears with a current focus in estuarine water ualit and carbon ccling

ontact nforation race chafer, niersit of loridahitne aborator, 55 cean hore ld, t ugustine, ,

153 12th International Symposium on Biogeochemistry of Wetlands

ETTLIN AND ENTRAINENT RERTIE ARTICULATE IN TE TA eona ino, he B eu, ad uate, Kn, ad ere, en re, arre th, on aunder, and ere hoa lorida nternational niersit , iai, , lorida ulf oast niersit , ort ers, , r orps of ngineers, ngineer esearch eelopent enter , icsburg, , 4outh lorida ater anageent istrict , est al each, ,

erglades torwater reatent reas s are flowthrough constructed wetlands operated to reduce phosphorus fro canal water draining the erglades gricultural rea hosphorus is reoed in the s ia ultiple echaniss including i plant and icrobial uptae, ii biologicall ediated cheical changes enhancing seuestration eg coprecipitation with calcite, iii particulate settling and entrapent, and i b cheical echaniss eg sorption, all resulting in storage ia accretion espite reoal successes 5% reduction in loads additional efforts are necessar to achiee regulator liits, including was to reduce particulate discharge which is generall 5% of outflow total he focus of this stud was to inestigate the dnaics of particulate resuspension in s and 4 article dnaics were studied relatie to applied shear stresses in core eperients, under stagnant, low, and high flow conditions, and as affected b differential flow paths and hdrologic and eteorological conditions

ultiple replicate soil cores were collected at the inflow, idflow, and outflow areas along northtosouth flowpaths in cells doinated b suberged auatic egetation ne set of cores were sectioned into c increents and analed for selected phsicocheical properties ores generall showed recentl accreted soil ie soil accreted since operations began depths of approiatel 5 c, ineral contents of % ash b , and contents that generall decreased down core and downstrea ther sets of replicate cores were subected to erodibilit treatents to deterine the near bed critical shear stresses that would cause erosion and entrainent using two ethods edlue and chabers edian critical shear stress of surficial sedients generall aried between and a b both ethods, were generall lower in the edlue copared to the , and were estiated to be approiatel orders of agnitude greater than the shear stresses eerted b the water colun suggesting little erosion andor resuspension of preiousl deposited sedient under ost flow conditions oweer, flow elocities and direction and the aount of particulates oing through the water colun were shown to be influenced b cell topograph, location, and wind speed and direction igh elocit c s were periodicall obsered in renant agricultural ditches especiall in proiit to cell outflows ocalied flows and water colun particulates were shown to be effected b wind speed and direction here was generall greater water borne particulates in the afternoon corresponding to dail highs in wind speed s the critical shear stress of the water colun were shown to be uch less than reuired to resuspend sedients we suggest that water oeent is suspending flocperiphton fro the surfaces of the his conclusion is also supported b increasing particle sie fro inflow to outflow along the cells

r en cinto is an associate professor in the epartent of arth and nironent and outheast nironental esearch enter at oring in the erglades sste for 5 his research focuses largel on the effects of hdrologic and nutrient ariation on biogeocheical processes

ontact nforation eonard cinto, h, epartent of arth and nironent 5, lorida nternational niersit, th treet, iai, ,

154 April 23-26, 2018 | Coral Springs, Florida, USA

TE RLAND EATERL WETLAND – EAR CNTRUCTED UCCE ak ee, ar orean, Kathrn aton it of rlando – ater eclaation iision, hristas, lorida

he rlando asterl etlands , copleted in , is a 4 hectare free surface flow constructed wetland he sste is coprised of different flow pathwas, treatent cells and water control structures he wetlands are peritted to receie up to illion d annual aerage dail flow he polishes ecess nutrients fro reclaied water receied fro the ron ridge egional ater eclaation acilit ro through , the sste has reoed 55,55 g of nitrogen and , g of phosphorus he aerage and concentration discharged fro the sste are g and g, respectiel

o aintain the uptae capabilit, the sste is anaged to proote dierse auatic habitats erbicide applications, water leel anipulations and prescribed burns are routinel eploed to help proote beneficial natie egetation such as cattails ha hen detritus significantl accuulates, full scale uc reoal proects are initiated etween and , nearl hectares hae been renoated with approiatel 5 illion cubic eters of uc reoed hese proects reduce short circuiting within the wetlands and create conditions for iproed wildlife habitat and nutrient uptae

he continues to proide a site for arious undergraduate and graduate research urrentl, the lorida ish and ildlife onseration oission along with the niersit of lorida are studing the conditions that affect giant bulrush hoenoetu aornu suriorship in restoration areas niersit of entral lorida facult and students are researching arious topics such as uric acid concentrations and its possible correlation with wildlife how constructed wetlands and nutrient gradients affect greenhouse gas production and bacterial species identification and its influence on and reoal

he sste was also designed to proide wildlife habitat o help achiee this, oer illion natie auatic plants were installed iodiersit within the is high with ore than 4 species of birds being recorded within the constructed wetlands eeral ears after construction, the was opened to the general public as the rlando etlands ar nnuall, tens of thousands of isitors freuent the ar ird watching, photograph, hiing, ogging and horsebac riding are popular actiities olunteer group has been fored to help with wetlands education and assist with ar operations ro nutrient reoal to public awareness, the continues to sere as a odel for constructed wetlands

ar ees is etlands anager for the it of rlando’s Water Reclamation Division. Mr. Sees has more than 20 years of eperience anaging and aintaining the rlando asterl etlands

ontact nforation ar ees, it of rlando, 555 heeler oad, hristas, lorida, ,

155 12th International Symposium on Biogeochemistry of Wetlands

el evai, ohn Kono,, aro oronadoona, tehen a, en aer,, tee Ke, ana ae,, ad udn, aoa antaara, red ar, an roer,, Benan on, lorida nternational niversity Miami lorida S 2Soth lorida Water Manaement District West alm each lorida S verlades ondation almetto ay lorida S Sotheast nvironmental Research enter Miami lorida S verlades ational ar omestead lorida S

verlades coastal etlands are eosed to saltater intrsion from storms and sealevel rise SR leadin to ncertainties for the fate of elorond caron . Soil microial etracelllar enyme activities s drive oranic matter readon. r oectives ere to determine ho microial s and root readon in freshater and racish ater eat soils are affected y saltater intrsion. We simlated eisodic saltater intrsion y monthly n tu dosin of etland chamers n .2 m diameter ith nstant cean. ontrol etland chamers n ere dosed ith amient site ater. We deloyed root litteras in racish and freshater chamers for aroimately 2 years retrieved after 2 and 2 months. fter each collection e analyed the remainin root litter for microial s elemental stoichiometry nitroen hoshors and readon rate.

t the freshater site root deradation as 0 ithin the first 0 days for oth control and treatment chamers. fter 0 days the deradation rates sloed don ithin oth the controls and treatments ended at arond 0 remainin after 0 days. t the racish site root deradation as also 0 ithin the first 0 days for oth control and treatment chamers. fter 0 days the ercent dry matter remainin slihtly decreased at oth the controls and treatment chamer ith dry matter ended at arond 0 remainin after 0 days. he maority of mass loss occrred ithin the first 0 days. nterestinly the maority of mass loss occrred ithin the initial 0 days.

We imlemented a ath analysis aroach to antify the relative imortance of hyothesied factors contritin to root readon rate after short 0 days and lonterm 0 days drations at oth the freshater and racish site. We constrcted for ath models ith eleven redictor variales for root readon averae hoshoros slfr S and acirin enymes salinity litter litter oreater dissolved oranic D oreater total dissolved D oreater solle reactive SR and S . reliminary reslts from the ath model indicate redictors 0.0 of readon rates vary eteen shortterm and lonterm eosre and eteen sites. reshater shortterm readon rates ere est redicted standardied coefficient directly y laile acirin enyme activity 0. salinity . D 0.0 SR 0. and S .0. reshater lonterm readon rates ere est redicted y salinity .2 D 0. D 0. SR 0. and S 2.2. racish shortterm readon rates ere est redicted y 0. and S 0. acirin enymes content 0. D 0. D 0. and S 0.0. racish lonterm readon rates ere est redicted y salinity . D .2 SR 0. and S .2. verall enyme activities ere not redicted to have direct effects on readon rates lon term. oreater chemistry articlarly S aears to e a ood redictor of readon rates drin shortterm and lonterm incations and across sites. Site chemistry dominates readon rate liely ecase it oth directly inflences leachin and also may indirectly affect enyme activities. verae enyme activities across root liter collections may not e effective redictors of readon as they reresent instantaneos microial demand. Dosin eeriments rovide insihts into critical eat formation and deradation mechanisms and ill allo for etter lannin and ater manaement decisions.

Shely Servais is a h.D. candidate at lorida nternational niversity. Shely ill defend her dissertation in Smmer 20 and is interested to hear aot any o or ostdoc oortnities

ontact nformation Shely Servais Deartment of ioloical Sciences lorida nternational niversity 200 SW th Street Miami S

156 April 23-26, 2018 | Coral Springs, Florida, USA

ala oemake and ran nderon S eoloical Srvey Davie S 2S eoloical Srvey Sacramento S

n forested etlands caron cyclin overns eat soil accmlation driven rimarily y the difference eteen ecosystem hotosynthesis and resiration as ell as lateral advection of aterorne caron and methanoenesis. or coastal etlands facin sealevel rise a frameor as created for estimatin otential elevation chanes ased on for years of continos cyclin data ithin yress Sam Darf yress and ine land ecosystems in the reater verlades. Soil accmlation as estimated sin a todimensional massalance model of vertical net ecosystem caron echane vertical fl of methane and lateral flos of dissolved oranic D and inoranic caron D in srface ater that drained a stdy area.

he seestered rohly 20 metric tons of from the atmoshere over the for year stdy. his vale as comted as a smmation of daily yress Sam Darf yress and ine land rates eihted y the satial etent of each ithin the stdy. roimately 20 metric tons of as emitted from these ecosystems as methane drin decomosition. et lateral aterorne advection of D and D contrited aot 22 metric tons of into the stdy area as oranic and inoranic material. n accmlation of aot mm of soil as estimated y the massalance model over the for year stdy assmin an averae soil ldensity vale of 0.2 cm. ccmlation rates ere very sensitive to chanes soil l density vales. otential annal elevation ains from the caron cycle ere 0. mm er year or aot . mm er year less than the mean tenty year rate for sealevel rise 2. mm er year measred y the SS at aylor River in the verlades.

arclay Shoemaer is a research hydroloist for the SS. is interests inclde comtational hydroloy sstainale ater se climate chane and hydroloy and hydroloic characteriation for local and reional conflicts over ater. e has athored nmeros SS technical reorts and ornal articles and has served as revieer for several scientific ornals.

ontact nformation W. arclay Shoemaer S eoloical Srvey enter for ollaorative Research 2 ollee ve Dave S

157 12th International Symposium on Biogeochemistry of Wetlands

– oa imon, a eer, and odd orne, Whitney aoratory for the Marine iosciences St. stine S 2Wetland ioeochemistry aoratory ainesville S Smithsonian nvironmental Research enter deater MD S

loally coastal etland veetation distritions are chanin in resonse to climate chane. n the sotheastern nited States increased inter temeratres over the ast decades have reslted in oleard eansion of manroves at the eense of saltmarsh alterin manrove rodctivity caron fles and ecosystem storae. he manitde and direction of chane ill e deendent on environmental variales and ntrient enrichment is amon one of the ey drivers of rodctivity in manroves. lteration of caron dioide 2 effl and decomosition de to etrohication has the otential to alter dynamics of marine ecosystems crrently nderoin chane. We measred 2 effl elorond decomosition and soil in the manrove – salt marsh ecotone in St. stine lorida to estimate 2 fl rates and elorond decomosition in a nitroen limited ecotonal ecosystem and 2 elcidate the effects of ntrient enrichment on ecosystem storae. trient enrichment reslted in an increase in manrove iomass and soil 2 effl and a decrease in elorond decomosition of cotton stris. Soil as sinificantly different across treatments. onseently e assert that the net chane on ecosystem stoc and fl is deendent on ntrient stats of the system.

Dr. Simson is an ecoloist hose research occrs at the intersection of ecosystem rocesses and the iotic and aiotic factors hich inflence them. She is crrently focsed on storae and fl in the manrove – salt marsh ecotone and ho natral ertrations alter the dynamics of the system.

ontact nformation ora Simosn 0 cean Shore lvd St stine 200

158 April 23-26, 2018 | Coral Springs, Florida, USA

amaa iová Eliška Rejmánková and aroaa Koro

Hdra ertata adohora hoenoetu aornu Hdra ertata hhorna rae

159 12th International Symposium on Biogeochemistry of Wetlands

oe moak Ran oe avi aomaino aa R Raaa a E Roeneim aoln ae ia ame aa an oa eia an iian ane

ard’s LiDAR Hyperspectral and Thermal (G

160 April 23-26, 2018 | Coral Springs, Florida, USA

le R m eane oeke an m in il and ater ciences Department niersity lrida Hmestead L A ansas ilical rey niersity ansas Larence A

Greenhse as (GHG emissins rm etlands are aected y sil yen ( temperatre and mistre r ater illed prespace ( rrent samplin methds ail t captre h shrtterm lctatins in these driers aect GHG emissins leadin t inadeate estimates annal emissins rm etlands Althh methane (H and nitrs ide ( les hae een measred r simlated at dierent spatial and tempral scales ln term GHG measrements and accmpanyin enirnmental data are needed t redce ncertainty and identiy ey driers as emissins e cpled eely methane (H and nitrs ide ( l measrements ith minte interal sil sensr data r aterilled pre space ( calclated rm sil mistre and temperatre t inestiate hich sensr est eplained GHG emissins hether cntins sensr data eplained emissins eynd measrements synchrnied ith eely les and hat ariales est eplained and predicted GHG les Data rm sites cllected eteen rm cnstrcted etland in Daytn H ere sed t deelp timela mdels ased n predeined time interals prir t the l measrement and instantanes mdels ased n sensr readins cncrrent ith GHG l measrements

The mst imprtant predictr r H l as the ariance in sil hrs prir t the l measrement Hih H les ccrred hen ariance as lare de t a transitin rm l t hih sil This indicates that shrt term lctatins in sil related t recnnectin the sils t the atmsphere typical sil drainae reslt in H emissins les ere est mdelled ith data rm the sil mistre sensr and the mst imprtant predictr as the days prir t the l measrement Dry cnditins lead t emissins as pea les ccrred days ater the lest minin data rm mltiple sensrs eplained mre ariance in les than tp sinle sensr mdel r each as ( r H and r nderscrin the relative importance of interactions among drivers. However, the most important variable (based on ∆AIC) did nt chane eteen the indiidal and mltiple sensr mdels les predicted sin linear interplatin ere siniicantly dierent rm mdel predictins The larest dierence eteen the t predictie methds ccrred ater perids rapid chane indicatin linear interplatin may nderestimate les r stdy demnstrates that lnterm cntins data hich accnts r shrtterm ariatin in sil prperties can impre r nderstandin etland ecsystem nctin

Dr myth is an Assistant ressr iechemistry at the niersity lrida he has a hD in arine cience rm hapel Hill and as a pstdc at the niersity ansas and a Daid H mith nseratin ell at the irinia nstitte arine cience

ntact nrmatin Ashley R myth il and ater ciences Department Trpical Research and dcatin enter niersity lridaA th t Hmestead L

161 12th International Symposium on Biogeochemistry of Wetlands

lina ea on R ie ili al Ron oanje an aio eonei Lisiana tate niersity atn Re LA A ranield niersity ranield

arsh lss is a mar cncern in the ississippi Rier Delta and is a reslt many actrs incldin sediment r ntrient depriatin and salt intrsin reshater diersins are desined t reintrdce reshater rm the ississippi Rier in rder t maintain reshater marsh eetatin and ntrient cyclin nctins ne aspect preentin marsh lss is accretin hich can e drien y mineral sedimentatin r the ildp ranic matter ranic matter accretin is drien y plant prdctiity hereas ecess decmpsitin can neate ains in marsh eleatin th prcesses are limited y aailale ntrients This stdy characteried sil prperties at a reshater diersin site ere ll reshater l t the site ean ith the intentin resamplin ater a decade diersin peratin e present a aseline ntrient stats and accretin prcesses this marsh ere reshater sediment and ntrient inpt ccrred There ere statins ( and cm depth t determine a aseline sil mistre l density (D pH ranic matter cntent ( ttal phsphrs (T nitren (T and carn (T aps sil prperties in the recently accreted t cm depth interal ere prdced sin riin rm the spatial crrelatin analysis e sered that T T and in the t cm depth interal had similar prperties and ere clsely inersely related t D These reslts alled s t iner the mechanism accretin acrss the etland hich aried ith distance rm the rier ntinin stdy Dais nd reshater diersin area is nin and a samplin team ill retrn in April and ay t sere the chanes in iechemical prcesses accretin plant ntrient ptae and cmmnity strctre Lnterm eects reshater restratin prects hae yet t e antiied and this research can reeal the ptential impacts reshater diersins n reshater marsh sil prperties related t resilience in castal Lisiana

BIO: Alina Spera is a master’s candidate in the Wetland and Aquatic Biogeochemistry Laboratory at Louisiana State University. Alina’s research cses n the impacts lare scale restratin prects n reshater etland iechemical prcesses

ntact nrmatin Alina pera Lisiana tate niersity ast nirnment ildin atn Re LA A

162 April 23-26, 2018 | Coral Springs, Florida, USA

avalen einmlle le ime on R ie ia ame Aquatic Biogeochemistry Laboratory epartment o Biology University o entral lorida Orlando L USA errestrial Biogeochemistry Lab ubenstein School o nvironment and atural esources University o ermont Burlington USA Wetland and Aquatic Biogeochemistry Laboratory epartment o Oceanography and oastal Sciences Louisiana State University Baton ouge LA USA

oastal etland submergence can occur hen rates o relative sea level rise eceed that o vertical accretion or landard transgression. Once collapsed and disarticulated by etland submergence these highly organic soils are eposed to oygenrich seaater hich may accelerate mineraliation rates. o determine ho inputs o oygenated seaater alter degradability o organic matter ith depth nine soil cores ere collected to a depth o m rom three bracish marshes ithin Barataria Bay LA USA hich is eperiencing high rates o etland loss from submergence. Field enzyme activity (βglucosidase B acetylbetaglucosaminidase A alaline phosphatase (AP), βxylosidase (XY), and βcellobiosidase B and microbial biomass carbon B did not change signiicantly ith depth until cm here activity increased dramatically and then sloly declined. otal carbon total nitrogen and percent organic matter ere highest beteen and cm contradicting the generally accepted paradigm o decreasing litter quality ith depth. olloing initial characteriation soil microcosms ere created or depth segments under anaerobic conditions mimicing an intact etland and aerobic conditions mimicing a submerging etland miing ith oygenated bay ater carbon dioide O production as measured ithin the bottles over days. arbon dioide production as greatest ithin the aerobic treatments. At cm the dierence in O production beteen aerobic and anaerobic treatments as greater than O production at the soil surace. hough O production increased ith depth ithin both treatments potentially mineraliable ammonium rates decreased ith increasing depth. esults rom this study challenge the assumption that both organic matter quality and degradability o organic matter decrease ith depth suggesting coastal etland submergence could signiicantly enhance O emissions.

BIO: avalend Steinmuller is a h andidate at the University of Central Florida in Dr. Lisa Chambers’ Aquatic Biogeochemistry Lab. Her research ocuses on biogeochemical responses o coastal etlands to sea level rise.

ontact Inormation: avalend Steinmuller epartment o Biology University o entral lorida Orlando L

163 12th International Symposium on Biogeochemistry of Wetlands

avi mne o aam an Ro inkle U.. eological urvey Florida ater cience Center Lut Florida UA anaai henua – Landcare esearch Lincoln e ealand University of Central Florida rlando Florida UA

reservation of eatland carbon stocs has global and local imlications. lobally loss of eatlands is coincident ith the release of greenhouse gases. Locally eatland loss can result in marsh subsidence and associated hydro ecological imacts as ell as the release of sequestered nutrients to donstream ater bodies. Atmosheric landscae carbon echange as monitored at a subtroical eat marsh in Florida UA from using eddy covariance methods. During the study eriod hydroeriod ranged from nine to telve months. et ecosystem roductivity as strongly affected by hydroeriod varying from relatively lo g CC m y in years ith eriodic drying events to much higher values g CC m during years ith constant marsh inundation. Loer rates ere rimarily the result of increased eat oidation during eriods hen marsh ater level as belo land surface. ethane emissions varied seasonally ith temerature and ater level and averaged g CHC m y . radeoffs eist beteen toograhic reservation of etlands and reduction in the otency of greenhouse gas emissions. nundation simultaneously reduces carbon mass loss through suression of eat oidation but increases the release of methane a highly otent greenhouse gas. A rescribed fire in consumed aroimately ercent of the aboveground biomass g C m raidly releasing carbon sequestered over a eriod of years.

B David umner is the Associate Director for tudies of the U CaribbeanFlorida ater cience Center he has years of hydrologic eerience ith the U in Florida orth Daota and ississii.

Contact nformation David umner U CaribbeanFlorida ater cience Center et Lane Lut FL UA

164 April 23-26, 2018 | Coral Springs, Florida, USA

E EER aina aamake e ae an al lek

eeoima akaio kaio aninea oinoi

165 12th International Symposium on Biogeochemistry of Wetlands

Eik aeol olin ane e eman i anen

166 April 23-26, 2018 | Coral Springs, Florida, USA

R R aiko eaaa enji amaa aai akaa ieio ika

ln jaonia ain mania

jaonia jaonia – mania mania –

jaonia – mania – –

jaonia mania jaonia mania

167 12th International Symposium on Biogeochemistry of Wetlands

oen ooooni n e le ae an an akovi

allineia ameiana illa veiillaa harvested to record wet and dry weights. Results showed that Peat and Sand did not substantially impact plant’s ass production. Vallisneria’s growth was not affected by different salt types in various salinity as it showed a similar effect on plant’s biomass as Sea Water.

Mohsen Tootoonchi has a Master’s degree in Soil and Water Science from the University of Florida. He is working Gettys’ Aquatic Plant Science Lab at the same institution. His dissertation research examines the effect of saltwater encroac

168 April 23-26, 2018 | Coral Springs, Florida, USA

Roe R ille epartment of ceanography and oastal Sciences ollege of the oast and the nvironment Louisiana State University aton Rouge Louisiana USA

Mangroves store larger amounts of carbon per area of coastal one than areal estimates in many terrestrial ecosystems and are suggested as a meaningful strategy to mitigate the effects of greenhouse gases. However analyses of how ecosystems may mitigate the global carbon cycle also require mapping ecosystem area with respective storages and fluxes across the earth surface to compare annual global carbon flux among ecosystem types. We provide a new framework based on diverse coastal environmental settings ie diverse estuarine morphology to explain global variation in mangrove carbon storages using soil organic carbon as a model to more accurately determine mangrove contribution to global carbon dynamics. We deliver the first global mangrove area estimate occupying distinct coastal environmental settings highlighting the role of carbonate settings as global blue carbon hotspots where S stocks have been underestimated by up to . arbon budgets suggest that a large fraction of mangrove organic matter is buried or consumed locally and the importance of mangrove detritus is related to the ratio of wetland area to open water. xport of mangrove with an approximate of is less than previously thought and much of the exchange via tides may actually be equally distributed among dissolved organic and inorganic carbon. Wood production is about g myear and the average sequestration in mangroves soils is about g m year. The sum of these two measures PPW ∆Sorg is then a mean approximation of mangrove P. Geographic location and regional climate of maor mangrove forests may explain carbon exchange with nearshore coastal waters e.g. the wet tropics compared to drier low latitude regions.

oastal environmental setting also affects the form e.g. and magnitude of carbon exchange between mangrove forests and nearshore coastal waters. T in the net tidal exchange from mangroves to the estuary becomes a depending on how much of this organic carbon is respired back to the atmosphere. Most estuaries are considered heterotrophic and thus much of this mangrove carbon is not buried in the coastal one. This is still a maor question in the carbon budget of coastal ecosystems but there have been some very interesting discoveries in the last decade. losing the gaps in these nutrient budgets is very important and need to be linked with estimates on how such exchanges vary with disturbances on ecosystems associated with land use and pulses such as floods fires and cyclones when estimating how the biogeochemistry of mangroves may mitigate global nutrient cycles.

r. Twilley is xecutive irector of the Louisiana Sea Grant ollege Program and professor in the epartment of ceanography and oastal Science at LSU. Most of r. Twilleys research has focused on coastal and ecosystems and more recently has been involved in developing ecosystem models coupled with engineering and landscape designs to formulate adaptation strategies for coastal communities. He received his S and MS from ast arolina University Ph from University of Florida and postdoctoral studies were at University of Maryland enter for nvironmental Studies.

ontact nformation Robert R. Twilley epartment of ceanography and oastal Sciences ollege of the oast and the nvironment Louisiana State University aton Rouge Louisiana USA

169 12th International Symposium on Biogeochemistry of Wetlands

RR ieoi akaa a leen ean E eene aell ei an ee ielen epartment of Marine and cological Sciences Florida Gulf oast University Fort Myers FL USA epartment of hemistry and ioscience Aalborg University Aalborg enmark

iooia la is a recently proposed new species of cluster iooia isolated from freshwater sediment. la and other iooia play a key role in the global nitrogen cycling in the first process of nitrification conversion of ammonia to nitrite. iooia and its sister ioomona belong to the family itrosomonadaceae within the class etaproteobacteria. The functional redundancy and niche separation created by iooia and ioomona species and later with ammoniaoxidiing archaea has become a globally popular subect in environmental microbiology. Although they have been concurrently found in various natural and manmade environments the dominance of either group in specific habitats may be used as a biological indicator of environmental health. cologically iooia dominate in various soil environments but minor in wastewater treatment plants. urrently recognied iooia species do not contain high ammonia adapted species and this oligotrophic nature may lead to an ecological advantage of this group of nitrifying bacteria in nature.

The obective of this study is to understand what is the inventory of genes necessary and unique for the habitat differentiation and sharing of la and other betaproteobacterial ammonia oxidiers through complete genome sequencing. The genomic data were exploited in examining the global distribution and habitat range of la.

The complete . Mb circular genome comprised proteincoding A sequences tRA genes and a single SSS rRA operon. The gene inventory supported chemolithotrophic metabolisms for function in wetland and soil environments. The APG genome encoded a large number of chemotaxis and flagellumassociated proteins suggesting the importance of chemotaxis for la. Many but not all ammoniaoxidiing bacteria use urea for chemolithotrophic growth. The ability to use urea as an energy source conveys an advantage to ammonia oxidiers including pH manipulation and as an alternate energy source. The presence of urea may also allow these microorganisms to survive at low pH conditions because the maority of ammonia is in the form of ammonium ion which is not directly used by ammonia oxidiers. A urease operon was present in the genome of la. Additionally a gene coding urea A transporter substratebinding protein was located in the genome. t is known that the disruption of this coding gene leads to the loss of highaffinity urea transport activity in cyanobacteria. This protein was found in all iooia genomes but not in the genomes of ioomona. The prominence of iooia in acidic soil can be partially explained by the presence and absence of this protein which assist in urea uptake and metabolism. Furthermore the gene for polyphosphate kinase k were present in the la genome. This enyme has been shown to be involved in stress tolerance biofilm formation and virulence through the utiliation of poly P to generate guanosinetriphosphate GTP. We found that k is widely present in other iooia genomes but not in the genomes of ioomona. Thus this is another example of genes that can potentially assist the environmental adaptations of iooia. espite a limited phenotypic diversity iooia has a wider variety of morphology. All iooia species are motile this is in contrast with ioomona species in which nearly half of species have no motility. Unique morphology along with chemotaxis and biofilm formation capability supported by PP may increase the ecological fitness of iooia in microenvironments. ur global search of lalike gene sequences in environmental clone and metagenomic libraries revealed the habitat range and global distribution patterns of this species indicating that la is a cosmopolitan species and widely distributed in temperate freshwater environments e.g. lake sediment rice fields wetlands. la represented the greatest numbers of environmental ammonia monooxygenase gene amo sequences among currently recognied iooia species and is regarded as the most cosmopolitan species.

r. Urakawa is an aquatic ecologist of FGU with more than years of experience culturing and studying nitrifying microorganisms. He is interested in environmental restoration and has engaged in various research proects including natural and constructed wetlands.

ontact nformation Hidetoshi Urakawa Florida Gulf oast University FGU lvd. S. Fort Myers FL USA

170 April 23-26, 2018 | Coral Springs, Florida, USA

eia aae on R ie Rajnee aal an vini aan Louisisana State University aton Rouge LA USA Florida AM University Tallahassee FL USA

n April the P eepwater Horion oil platform in the Gulf of Mexico experienced an explosion that resulted in the largest marine oil spill in U.S. history. As a remediation tactic . million liters of dispersant orexit A was sprayed into Gulf of Mexico waters. t has been shown in previous studies that exposure of coastal wetland soil to orexit can lead to the reduction in several important microbialmediated ecosystem services related to water quality denitrification and ecosystem primary productivity nitrogen mineraliation. To get a more complete picture of how orexit impacts coastal marshes it is vital to look at how the microbial communities in the soil react to different levels of orexit exposure. t is possible to find a link between shifts in the microbial community and changes in ecosystem services by analying changes in microbial activity and community structure over time with orexit exposure. Heterotrophic microbial respiration was measured for both wetland soil and estuarine sediment in arataria ay LA. USA. Triplicate cores for wetland soil and estuarine sediment were collected and analyed for microbial biomass carbon and nitrogen L moisture content TP and TT. There were four treatment levels control of orexitwet soil ratios under anaerobic conditions. Gas samples were analyed on a Gas hromatograph F to monitor microbial respiration over time. The samples were then analyed for microbial community structure using a metagenomics approach. These results will indicate how microbial activity and community structure in coastal wetland soils shift in response to orexit exposure. This research may provide more insight on how coastal wetlands will respondrecover over time after exposure to dispersant.

essica Vaccare is a graduate student in the Wetland and Aquatic iogeochemistry Laboratory at Louisiana State University. essica’s research focuses on ecosystem services and nutrient cycling in coastal wetlands.

ontact nformation essica Vaccare Louisiana State University nergy oast nvironment uilding aton Rouge LA USA

171 12th International Symposium on Biogeochemistry of Wetlands

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as i as a i a s ass a cs s s a as c ass a as ss a s a s issis ca c si issis i sia aas aaa a s is ca i s si asaaaa asi i s a asi cais s aas s a ia si aiia leoa as ic a as ia scis ii a asi a issis a asi c s a siiica as

a a si sac s as a sis s is a i sa iaa a aa sis a aa a is i ai s i s cas aa ss a si a c i ia cas sa i cs i a as ass ic as is a si i ai ai a ai as s cc cas as

a leoa s s cas i i a s a sii ca i si s i ass as s a s cas c acci a ciica isi i s ii a sa a aa aas ai s a a s s ai acci

ass i aas s a as a scia leoa ca i a aiis i a a ii leoa ss acss a asi as s s is a scis c s a a aii a a asi

a a is a assisa sac ss i is a isi ia a as sac icica asi iacis i ica is scia a asi as as

ac iai s a a is isi ia a cs

172 April 23-26, 2018 | Coral Springs, Florida, USA

oo van aen an in ailloio isi ia cs ia a isi

aci a assi as as s c s a siiica i css issis scia aas a ss as a assi as s siai s scis a aca iss ic ca as a a is si as iis sa as s ca as ssaia as iss si sis as a asi cais a scis ic scis s a a s sca s acss a asi sa a scis i a a acs i s issis

a a si sac s as a sis s is a i sa iaa a aa sis a aa a is i ai aas iii a scis i ss a a c i a s s scis sc s a s aa as s s as s s a a scis s i s cas aa ss a si a c i ia cas sa i cs i a as ass ic as is a si i ai ai a ai as s cc cas as s ii is c a si sac as i sa a scis aiia leoa a oam jaai a i a Eee oleaea iis i scis i iaca a aaca csis acss sis i s is ass as scis ii s aiis i i ssaia a

sc aca iss a as a s iica issis a scis i aca a as s ai aca is a ai a aas cs a ai

a a is a assisa sac ss i is a isi ia a as sac icica asi iacis i ica is scia a asi as as

ac iai s a a is isi ia a cs

173 12th International Symposium on Biogeochemistry of Wetlands

iine anomeen ao ekoi on R ie an anie ie i sac a ics isiaa a isi a

c is as cs a sai cs a ic a i a si ai a ai as sacs ca a ss asscia i isi sa ass cia sss a i casa siic sai c i a s ci c si a as aas a c si i a a as iss ii casa as i sai a a as cai i aca ai a iss aic ca a a as si ssi a ia si i i as ass a a c a i s a si ac sac siis icas a i as sis ai icia aci sai caaci i sis i ai i scs sc as ai i ai ci ai icica ss sai sss a i a si ac cis a sa as c aiaii ai ai s scs is a icia ciis i asac sis ca sai sais i icica is aai aa s i si ac assss c as cis i ss i a si aicais s icica ss si ia i s a i cas i ic sis i ai as i a saicai aciiis si icica csss i isi i sai ciss a s iciai css sics scia i s a ii is

a is a sac cis i i as ic i a sai a cai as si ic i a sac a as a i a sai cs cs a icis

ac ai isi a i sac a as ics

174 April 23-26, 2018 | Coral Springs, Florida, USA

Roio aa oe aa Raael oao isi aa a sac c a s icais isia aca aca ai

issis ai s ass a ss a si a cai c a aac s as issis a si sa cas is a saia as ssi a issis ss a ca iia i is s issis c a si siica csss ia ciis si a aa ss cis as a s ss a aac sis as s s a si i a i is a c i ic aa oiomi s a aac si cais i a s aa i i aic a si issis s siia sasa as i a aa si a s s a i a c s a ai a sasa scas a cass as ca c si is ss a ca sc i issis casi i i a s s a sci i aa sasa a a ca aisi i ia is a is cas si as a si i s s a is sasa a s is cas si si a s issis i s a sasa a aa c a is s issis i a cas i issis i s i si a s s issis cas i s i a siia as a a sci s sa si caaci sis c aia cac s issis s ss iica as a issis i si as a i is i ss acc a aac i s csss

aas is a sscia ss i sac ic isica acs a siaas iacis acss i ai s as ca i icais i a i ai as a ss i cis sa aia a iaia sciiic s a sciiic aiais

ac ai a a a i cics isi aa a

175 12th International Symposium on Biogeochemistry of Wetlands

– eik an oma iani o one iael iel an illiam enne a ica cics isi ia aisi i aa ai iscic isi ia si a s a ia a si isi ia aisi

Intertidal forests and salt marshes represent a major component of Florida’s coasts and are essential to the health and integrity of coastal Florida’s ecological and economic systems. I aii casa as a ci as i ici ca sis i i aii s ca i scas cis iia sss sa ass as a saass s aa a aic cs a ai i aii ac as ca sis a a cac as i sa ass a a ia icass i ca ssai as as s ca a sa ass

ia is cac as i sa ass is i a cass s a cici i a icas i a as sa cas i cs cc a as ia a i a s i c a cac as i ia sa ass as a icas i ca sa si cs ic a aia s i i as i is c ca sa i a ia a i c s cs i i s a ai iia ss c ic ss as as s ai ss ca is c i s ii asii as a a a i aas c c sa as ai as asiia as as a c a ss ca ii c ca a a as cs iias aai iics a i ia aa a as i a s i c is ss ca a a aai a c a a cas i ca scs

IO: Derrick Vaughn is a Ph.D. student within Thomas Bianchi’s lab at the University of Florida. His dissertation looks at dics i carbon storage along Florida’s northern coast in response to recent climate change and Holocene climate variability.

ac ai ic a iias a ai aisi

176 April 23-26, 2018 | Coral Springs, Florida, USA

i emelen aam ammon oe a H Bird esources Billings T U Billings entral atholic chool Billings T U

In today’s society, we are dependent on plastics. In 2015, we globally produced about 322 metric tons of plastic, and it is presumed that we could not cope with the removal of plastics from our society. However common plastics are nonbiodegradable and our use of plastics is polluting the world we live in. Polyethylene Terephthalate or PT is used in water and other drinking bottles synthetic clothing microwaveable trays insulation and more. hile PT is recyclable it is reduced to pollutants if littered. This is common considering PT is the most commonly used plastic. Many believe that this “plastic problem” cannot be helped.

eonella akaieni is a bacterium discovered outside a plastic recycling facility and it is capable of breaking down PT. It uses enymes called PTases to hydrolye PT into two monomers: ethylene glycol and terephthalic acid. t room temperature it can process a thin film of PT over the course of about days. This is a somewhat slow reaction time. However if the plastic is processed by means of essential cooking it it is more easily broken down. akaieni is easily grown and once it adheres itself to the surface of PT it can begin producing PTase and needs very little of other resources.

bacterial transformation was demonstrated as a fairly easy process in a lab using E oli to produce ampicillin resistance and many students were able induce insulin production in the culture with a second transformation. By isolation the PTase production gene E oli can be used to produce PTase and break down PT into environmentally friendly monomers more uickly and efficiently. large portion of our plastics can successfully be biodegraded and returned to the earth.

BIO: ic Vermeulen is a junior who currently volunteers at H bird esources. He studies bird behaviors as well as the microbial environment waterfowl ponds. His interest in this project was sparked by his aspirations in microbiology. ic has also been involved in several teaching groups giving presentations on microbial life.

ontact Information: icholas Vermeulen H Bird esources Billings T U

177 12th International Symposium on Biogeochemistry of Wetlands

i illaano ill in Re omia an al lian 1outh lorida ater Management istrict, est alm each, , 2niversity of lorida, ainesville, ,

he verglades tormwater reatment reas s are an important component of the verglades restoration efforts. he five s combined, have effectively reduced total phosphorus concentrations in the surface water, to as low as 20 g 1 and reduced loads, by as much as percent in the recent years. owever, the mandates to achieve lower concentrations at the outflow structures emphasie the need for a better understanding of the factors and mechanisms that might limit the s to achieve and sustain the current long term ater uality ased ffluent imit of 13 g 1.

his field investigation is a component of a comprehensive study to evaluate the sources, forms, flu, and transformation of phosphorus in the verglades s. pecifically, the investigation is being conducted along a transect in three different flowways with different vegetation types under stagnant, low flow, and high flow conditions. hosphorus cycling and movement within an is a function of various chemical, physical, and biological processes operating at different regions of the flowway. he concentration of observed at the outflow structure is a net result of all these processes.

reliminary results from flow events in 2 lowways s 1 and 3 show distinct concentration gradient from inflow to outflow at all phases of the flow event. verage reduction however, was higher for emergent auatic vegetationdominated 2 1 than for submerged auatic vegetationbased 2 3. In 2 3, concentrations were elevated under stagnant condition following a period of high loading but not after low loading with increases resulting from a large increase in particulate . eduction in accounted for most of the reduction in concentration along the treatment at all periods of the flow events. ompared to dissolved organic , soluble reactive was reduced much earlier in the , indicating that this fraction was consumed much more readily through biological and physicochemical processes operating along the front half of the treatment . esidual pool at the bac end of the was comprised mainly of and at all periods of the flow events. showed strong positive correlations with metal cations calcium, aluminum and iron along with total nitrogen, chlorophyll, total suspended solids, surface water p, and dissolved oygen during the low flow, high flow, and stagnant periods. he implications of initial results for operation and performance along with current efforts to identify the sources and composition of and especially at the lower reaches of the flowway will be discussed.

I r. illapando is currently a ead nvironmental cientist for the outh lorida ater Management istrict with almost 20 years of eperience managing water uality improvement proects in support of the ae eechobee and verglades estoration efforts.

ontact Information di illapando, outh lorida ater Management istrict, est alm each,

178 April 23-26, 2018 | Coral Springs, Florida, USA

ioen eeaam nton de om niversity of uriname, anica uriname and ulane niversity, ew rleans, ,

he study focusses on the occurrence of methyl mercury in sediments of ivers in the lowlands of entral and estern uriname. he use of mercury in the growing artisanal gold mining industry has caused an increase of mercury in the environment. his study tries to assess the accumulation of the mercury in ivers downstream and downwind of the gold mining areas. revious studies have shown that mercury accumulates in areas downwind of artisanal gold mining areas, but the process is not yet fully understood. his research has been ongoing for over si months, and up until now, assessed methyl mercury levels in sediments upstream, at and downstream of five villages. he study, which is part of a larger threeyear program, provides insight into the presence and possible accumulation of methyl mercury and its possible relations with seasonal variations temperature and water flow.

I I have a bacground in biological anthropology and ecology, with more than ten years of eperience in primate behavior and feeding ecology field studies. My current focus is on studying the impact of climate change on the distribution, availability, and methylation of mercury in the environment.

ontact Information rioen .. reedaam, h andidate nton de om niversity of uriname and ulane niversity, eeberg roet 3, anica uriname

179 12th International Symposium on Biogeochemistry of Wetlands

en oni an iain ie ie en anjie i an enlo en 1ey aboratory of eographic Information cience Ministry of ducation, ast hina ormal niversity, hanghai 20021, hina 2chool of eographical ciences, ast hina ormal niversity, hanghai 20021, hina 3resent address econd Institute of ceanography, tate ceanic dministration, anghou 310012, hina

stuarine and coastal area is significantly a contributor to the inventory of nitrous oide 2, but we now little about the effect of temperature increasing among different coasts. his study presents the results of incubation eperiments of the 2 flues along the ast hina stuarine and oastal line from south tropical site, 20 , to north temperate site, 035 , in which the temperature was controlled on three gradients 15,25 ,35 and the semidiurnal tide was simulated by episode flooding. uring the aeration, 2 flues from north sites showed an eponential positive correlation with temperature, with the mean 2 flues increased from .00. 2 1 2 1 nmolm h at 15 to 315.3355. nmolm h at 35 . owever, the outhern 2 flues decreased with temperature rising gradient, from the from the 233.221. nmolm2h1 at 15 to the 12.12.3 nmolm 2 1 2 1 h at 35 . In the period of inundation, northern 2 flues increased from 1.51.5 nmolm h at 15 2 1 to 1023.100.1 nmolm h at 35 with temperature gradient, and the outhern 2 flues decreased from the highest flues .0305.5 nmolm2h1 at 15 to .0.5 nmolm2h1 at 35 . oastal sediments from different climatic ones varied in responses to increasing temperature. nder global ongoing warming condition, high latitude coasts would act as important potential 2 sources. dversely, the flu of 2 from low latitude coastal sediments may decrease with the increasing temperature. In addition, the influence of the flooding ehibited negative correlation with increasing temperature among the mostly studying sites. emperature and tidal fluctuation have a combined impact on 2 budget at coastal wetland. nd, the balance of 2 production and consumption determine the varied 2 emission from different soils responding with increasing temperature.

I r. ang is a professor with 1 years of eperience on carbon and nitrogen biogeochemical cycling at angte delta. e has etensive eperience with greenhouse gases production and emission from river and wetland and the nitrogen removal by wetland, and has led proects.

ontact Information ongi ang, chool of eographical sciences, ast hina ormal niversity, 500 ongchuan oad, hanghai, hina 20021

180 April 23-26, 2018 | Coral Springs, Florida, USA

onjn an ak Rive i Riaon ue niversity etland enter, icholas chool of the nvironment, ue niversity, urham, ,

Iron is the fourthmost abundant element in the arths crust. It plays fundamental roles in regulating biological and physical processes in many ecosystems. tudies have showed that iron e can substantially stabilie organic carbon in mineralrich soils. Recently a study suggests that Fe even could protect carbon via an ‘iron gate’ mechanism during drought in organicrich wetland soil. Coupled with a “soilmoisture gate” theory that low soil moisture limits phenolic oidase activity and decomposition in unsaturated peatlands, it appears that 1 double gates against shortterm drought might eist in some organicrich soils, particularly peatlands and 2 minerotrophic fens with substantial e inputs liely present higher resilience to drought than oligotrophic bogs.

To test whether the ‘iron gate’ mechanism can protect carbon in organicrich wetlands, we 1 set up a manipulation eperiment by adding ferrous iron to a highlignin and lowe peatland soil, and 2 measured dissolved e in pore water from additional peatlands in orth and outh merica. ur results indicate that e actually stimulates carbon decomposition in peatlands during either flooding or drought, which is in line with other findings in humid tropical forest soil, but contrary to the ‘iron gate’ mechanism. In organicrich soils, phenolics principally control carbon decay. lthough droughtinduced e oidation reduces phenol oidase activity, thus decreasing the oidation of phenolics, simultaneously e oidation precipitates phenolics, which disables the carbon protection mechanism. he conflicting processes affecting the availability of phenolics in peatlands indicates that the ‘iron gate’ mechanism is probably not applicable in peatlands.

I r. ongun ang is a biogeochemist. e has been woring on biogeochemical cycles of , and in freshwater wetlands, especially in peatlands and riparianlittoral terrestrialauatic ecotone for more than 10 years. e has published more than 10 peerreviewed papers as first author in top ournals, including ature limate hange, tmospheric hemistry and hysics, tmospheric nvironment and ournal of eophysical esearch etc.

ontact Information ongun ang, ue niversity etland enter, icholas chool of the nvironment, o 0333, ue niversity, urham, 20,

181 12th International Symposium on Biogeochemistry of Wetlands

iin an tate ey aboratory of egetation and nvironmental Change, Institute of otany, Chinese cademy of ciences, eiing, China

ost forests worldwide are located in upland landscapes. pland forests are traditionally thought to be net sins for atmospheric methane C. revious studies have mainly focused on ground C flu in upland forests, and living tree stembased C processes and flues are thus relatively poorly understood. Recently, we measured in i C flues from the stems of living trees in upland forests using a static closedchamber method, and investigated the relationship between C concentration and water content in the heartwood of living trees. e found that approimately – of tree stems or approimately – of tree species had a substantial C concentration of ≥10000 μL L– in their heartwood across the midtemperate, warm temperate, and subtropical upland forests. The stems of ol aviiana emitted the large uantities of C, amounting to mean annual emissions of – μg m–h– during uly –uly on a stem surface area basis. The emission rates were similar in magnitude to those from tree stems in wetlands. The emitted C was derived from the heartwood of stems. n a aviiana dominated ecosystem scale, annual stem C emissions were euivalent to of the amount of C consumed by soils. The heartwood C concentration was related to water content by a power function. threshold of water content occurred beyond which C was produced at high levels in the heartwood. Temperature was not a limiting factor for C production during the summer and autumn seasons, and thus most of the C production may be eplained by water content in the heartwood of living trees in upland forests.

etailed results related to this abstract can be found in ang et al., ew hytologist –, doi .nph. ournal eophysical Research iogeosciences –, doi ..

I r. ang is a professor with etensive eperience in studying methane in terrestrial ecosystems, and has led more than proects dedicated to measuring and investigating the processes and flues of methane.

Contact Information hiing ang, tate ey aboratory of egetation and nvironmental Change, Institute of otany, Chinese cademy of ciences, anincun , iangshan, eiing , China

182 April 23-26, 2018 | Coral Springs, Florida, USA

iael R eel a eveone an ellie ion

enoom neimali

183 12th International Symposium on Biogeochemistry of Wetlands

on R ie ia R an oe aian

184 April 23-26, 2018 | Coral Springs, Florida, USA

enjamin ilon el evai ean ale iviana aei een E avi Eveln aie eve ell on ominoki ioe aen ennie Ria avi Rnik e kla oe aelek an ian ole

referred to as “peat collapse” and hypothesized to be the result of a rapid shift in soil C balance, leading to a net

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presence of live sawgrass “pedestals” in the brackish portions of the Everglades where it ap

185 12th International Symposium on Biogeochemistry of Wetlands

iao avannah iver Ecology aboratory niversity of eorgia, iken, C

he wetland syste was constructed to treat building process water and stor runoff water fro the ritiu rocessing acility on the avannah iver ite iken, C onthly onitoring of Cu and n concentrations, and water uality paraeters teperature, p, , alkalinity, C, sulfate, chloride in surface waters continued fro to ater saples were collected at the source pipes, retention basin, influent and effluent connected to wetland cells, and the discharge strea etal speciation was odeled by verall, the wetland syste functioned well otal Cu and n concentrations released to the effluent strea were below the E liit, and the average reoval efficiency was for Cu and for n ulfate copounds were consumed by the wetland during the “warm” season (Feb. to Aug.) since sulfate concentrations in the effluent were lower than the influent igh teperature, adeuate labile organic atters, and anaerobic conditions favored sulfate reduction that produced sulfide inerals to copletely reove etals fro the water column. However, sulfate compounds were produced during the “cold” season (Sept. to next Mar.) when the doinant reaction in sulfur cycling shifted to sulfide oidation elatively high concentrations of etalorganic coplees, especially etal and fulvic acid coplees were also eplored during this tie, deonstrating increasing reactions between etals and organic atters hus, adsorption to organic atter becae the priary process for reoval n suary, the etalreoval process in wetland was seasonal and regulated by sulfur cycling lthough the wetland continued to successfully reove etals during the sapling occasions, long ter sustainability still needs to be closely onitored n addition, the etal accuulation in the wetland syste ay cause negative effects to the surrounding environent as they are biologically reactive, highly bioavailable, and can be easily transferred to terrestrial ecosystes by trophic echange

Contact nforation r iaoyu u, niversity of eorgia, iken, C nited tates

186 April 23-26, 2018 | Coral Springs, Florida, USA

ian e Ea llen a kni in an ao an ei an i ey aboratory of oastal etlands iogeosciences, ingdao nstitute of Marine eology, hina eologic Survey, ingdao, . . hina aboratory for Marine eology, ingdao ational aboratory for Marine Science and echnology, ingdao, , . . hina School of the oast nvironment, epartment of nvironmental Sciences, ouisiana State niversity, aton ouge, ouisiana, SA Aarhus niversity, epartment of ioscience, lant iology, Aarhus, enmar

he carbon seuestration rate (S) in deltaic wetalands is associated with the nutrient balance, sediment (soil) accretion rate (SA), and geological and climatic conditions. o explore these relationships, micronutrients , , and concentrations and ages determined by either dating marpaleosol or radiometric dating with s and b from cores of both the ellow iver elta () and the iaohe elta (H) wetlands in and , repectively, were analyed. ith the exeption of a, the concentrations of , org, u, n, Fe, Mn, Mg, , Al, and hydrogen ions (H) were significantly higher in the wetland soils of the H than the , but the organic Ss were virtually identical at the two sets of sites, about g m– y– at sites above mean sea level (MS). SA and organic S at H sites below MS were about . times the corresponding rates at sites above MS. SA and total S were much higher in the than the H because of the much greater accumulation rate of a in the . he organic Ss were primary controlled by SA in both detaic wetand systems. However, organic Ss in the H wetlands were much more sensitive to the changes of SAs than in the .

io r. Siyuan e Senior Scientist and the irector of ey aboratory of oastal etland iogeosciences, hina eological Survey (S). She has been wored as a coastal wetland scientist with the nstitute of Marine eology, S for years. iogeochemistry and carbon cycle are her specialities certified by her more than ournal articles both in hinese and nglish.

ontact nformation Fuhou oad, ingdao, Shandong rovince (ip code ), . . hina

187 12th International Symposium on Biogeochemistry of Wetlands

ominik ak il Ro enik Ree oia olamme aalie enne i eeman ioia ne ele

ae aoinaa

er 15 years’ experience in freshwater and wetland

188 April 23-26, 2018 | Coral Springs, Florida, USA

i an an illiam i erlades etland esearch ar lrida lf ast niersity aples

acrphytes cnities play sinificant rles in the carn and ntrient dynaics f treatent wetlands and their deelpent is larely inflenced y the hydrlical reie his stdy inestiated seasnal ariatins f the acrphyte cnities f ha f ran rnff treatent wetlands at ha reed ar aples lrida y interatin realtie hydrlical data n the site with hihresltin aerial phtraphy and rnd trth field sreys eraphic infratin syste is sed t create acrphytes cnity plyns and t extract the plyns int ectrs fr the spatial analysis drin dry and wet seasns patial analysis shwed that the shallw areas were dinated y nine acrphyte cnities dinated y alia enilae Eleoai ellloe oneeia oaa and laim jamaiene which were planted in and anim een ikania anen and a spp which hae since clnied the wetlands he deepwater areas were dinated y maea ooaa a lea and anim een in the dry seasn acrphyte cnity strctre in the shallw areas chaned sinificantly in the dry seasn f arch 1 cpared t the wet seasn in cter 1 especially t re clniin y anim een ikania anen and a spp his stdy cntrites directly t nderstandin eetatin cnity dynaics drin dry and wet seasn shifts which will lead t eclically rirs anaeent f the treatent wetland eetatin t ptiie its als f water ality ipreent hydrlic anaeent and prtectin f aesthetics fr the par

cnwledent his prect was spprt y the llier nty he athrs sincerely appreciate the assistance y radate stdents and isitin scientists at erlades etland esearch ar in seeral field sreys f the reed ar wetland eetatin and hydrly

r han is ssistant irectr f erlades etland esearch ar at lrida lf ast niersity with re than 1 years f experience plannin and ipleentin wetlands cnstrctin prects he has extensie experience with wetland and rier restratin and has experience with watershed hydrly and dellin

ntact nfratin i han erlades etland esearch ar ayshre ire aples 11

189 12th International Symposium on Biogeochemistry of Wetlands

akle alone even eae alo liva nin ao eo a iina aamme 1lrida nternatinal niersity iai he niersity f laaa scalsa

e exained the relatinship etween extree eents and carn dixide exchane rates acrss ar ecsystes in erlades atinal ar hese plse eents are particlarly iprtant fr nderstandin hw freshwater arsh and anre dinated ecsystes will adapt t sealeelrise and cliate chane in this lw eleatin strpical wetland cplex rdctiity acrss the erlades peas near the cast anres where the spply f fr seawater is alanced y freshwater spplies rdctiity is reatest in anre frests 1 yr1 cpared t ln and shrthydrperid freshwater arsh ecsystes that 1 rane fr ein a sall sin t a sall srce f 11– 11 yr annally lthh cliate and hydrly are ar driers f ecsyste strctre and fnctin distrance reies ie drht extree fldin hrricanes lw teperatre eents and fire interact with hydrly t dify pplatins cnities and ecsystes acrss the castal radient ecent l i thern scillatin cycles 1 and lw teperatre eents 1 hae prided iprtant pprtnities t explre ariatin in ecsyste sensitiity t distrances and recery traectries in freshwater arsh and anre frests

n lrida flctatins in precipitatin patterns ccr with chanes in the l i sthern scillatin phases hile l i eents cccr with wetter than aerae cnditins a ia phases are assciated with drier than aerae cnditins nder nral cnditins shrthydrperid freshwater arshes hae hiher prdctiity rates drin the dry seasn while lnhydrperid freshwater arshes exhiit reater prdctiity rates drin the wet seasn his sitespecific seasnality in prdctiity is drien y chanes in hydrly and as a reslt phases either anify r te seasnal prdctiity rates a ia phases were cncrrent with increased prdctiity in shrthydrperid freshwater arshes cpared t l i and netral years he exceptinally wet year assciated with an l i phase led t reater prdctiity in the lnhydrperid freshwater arsh hen it ces t lwteperatre eents 5 th water leels and distance fr the cast inflenced ecsyste sensitiity hile the lnhydrperid freshwater arsh ained re carn drin lwteperatre eents de t the hiher liht leels anre frest had the reatest carn 1 lst 11 lwteperatre eent crss distrance types hydrly played a ar rle in deterinin the deree f expsre and therefre the sensitiity t distrance

r nin ha is a pstdctral assciate wrin n a prect reardin reenhse as eissin in the erlades wetlands e has years f experiences in stdyin wetland and exchane at ecsyste and plant scale

ntact nfratin nin ha epartent f ily lrida nternatinal niersity 11 th iai 1

190 April 23-26, 2018 | Coral Springs, Florida, USA

ia ei an eman olin ane iael anna Eik aeol i anen e kla

laim jamaiene

191 12th International Symposium on Biogeochemistry of Wetlands

Notes

192