PRODUCT INFORMATION NSD1 (human recombinant) Item No. 10757
Overview and Properties
Synonyms: AR267, KMT3B, Nuclear Receptor SET domain-containing protein 1, WHSC1 Source: Recombinant N-terminal GST-tagged protein expressed in E. coli Amino Acids: 1,700-1,986 (N- and C-terminal truncations) Uniprot No.: Q96L73 Molecular Weight: 60.2 kDa Storage: -80°C (as supplied); avoid freeze/thaw cycles by aliquoting protein. Stability: ≥1 year Purity: batch specific (≥60% estimated by SDS-PAGE) Supplied in: batch specific Protein Concentration: batch specific mg/ml Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis.
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1 2 3 4
250 kDa · · · · · · · 150 kDa · · · · · · · 100 kDa · · · · · · · 75 kDa · · · · · · ·
50 kDa · · · · · · ·
37 kDa · · · · · · ·
25 kDa · · · · · · ·
Lane 1: MW Markers Lane 2: NSD1 (1 µg) Lane 3: NSD1 (2 µg) Lane 4: NSD1 (5 µg)
Representaõve gel image shown; actual purity may vary between each batch.
WARNING CAYMAN CHEMICAL THIS PRODUCT IS FOR RESEARCH ONLY - NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE. 1180 EAST ELLSWORTH RD SAFETY DATA ANN ARBOR, MI 48108 · USA This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution. PHONE: [800] 364-9897
WARRANTY AND LIMITATION OF REMEDY [734] 971-3335 Buyer agrees to purchase the material subject to Cayman’s Terms and Conditions. Complete Terms and Conditions including Warranty and Limitation of Liability information can be found on our website. FAX: [734] 971-3640 [email protected] Copyright Cayman Chemical Company, 07/03/2018 WWW.CAYMANCHEM.COM PRODUCT INFORMATION Description
The nuclear receptor SET domain-containing (NSD) family of histone lysine methyltransferases are important enzymes responsible for maintaining chromatin integrity. The NSD family consists of three members NSD1, NSD2, and NSD3.1 Perturbations in the NSD family can lead to several diseases such as Sotos syndrome, Wolf-Hirschorn syndrome, and cancers such as acute myeloid leukemia (AML) and breast cancer.2 NSD1, which can methylate histone H3 at lysine 36 (H3K36) and H4K20, is a large protein with 2,696 residues.3 It has several motifs near the C-terminal region that have been shown to be essential for chromatin regulation. Furthermore, NSD1 contains a catalytic lysine methyltransferase SET domain and four zinc-binding PHD fingers.4 NSD1 gene translocations, which promote expression of oncogenic NSD1 fusion proteins, are frequently found in patients with AML and breast cancer.2,3,5 In contrast, inactivation of NSD1 in neuroblastomas and glioblastomas has been shown to result in tumor suppression.2,5
References
1. Li, Y., Trojer, P., Xu, C.F., et al. The target of the NSD family of histone lysine methyltransferases depends on the nature of the substrate. J. Biol. Chem. 284(49), 34283-34295 (2009). 2. Lucio-Eterovic, A.K., Singh, M.M., Gardner, J.E., et al. Role for the nuclear receptor-binding SET domain protein 1 (NSD1) methyltransferase in coordinating lysine 36 methylation at histone 3 with RNA polymerase II function. Proceedings of the National Academy of Sciences of the United States of America 107(39), 16952-16957 (2010). 3. Berdasco, M., Ropero, S., Setien, F., et al. Epigenetic inactivation of the Sotos overgrowth syndrome gene histone methyltransferase NSD1 in human neuroblastoma and glioma. Proceedings of the National Academy of Sciences of the United States of America 106(51), 21830-21835 (2009). 4. Huang, N., vom Baur, E., Garnier, J.M., et al. Two distinct nuclear receptor interaction domains in NSD1, a novel SET protein that exhibits characteristics of both corepressors and coactivators. EMBO Journal 17(12), 3398-3412 (1998). 5. Qiao, Q., Li, Y., Chen, Z., et al. The structure of NSD1 reveals an autoregulatory mechanism underlying histone H3K36 methylation. J. Biol. Chem. 286(10), 8361-8368 (2011).
CAYMAN CHEMICAL 1180 EAST ELLSWORTH RD ANN ARBOR, MI 48108 · USA PHONE: [800] 364-9897 [734] 971-3335 FAX: [734] 971-3640 [email protected] WWW.CAYMANCHEM.COM