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Universidade Federal De Goiás Doutorado Em Biotecnologia E Biodiversidade Programa De Pós-Graduação Da Rede Pró Centro- Oeste UNIVERSIDADE FEDERAL DE GOIÁS DOUTORADO EM BIOTECNOLOGIA E BIODIVERSIDADE PROGRAMA DE PÓS-GRADUAÇÃO DA REDE PRÓ CENTRO- OESTE BIOPROSPECÇÃO DE BACTÉRIAS A PARTIR DA COMUNIDADE ENDOFÍTICA RADICULAR CULTIVÁVEL DE Aloe vera (L.) BURM. F. (Xanthorrhoeaceae) Cintia Faria da Silva Doctor Scientiae RIO VERDE GOIÁS – BRASIL 2019 1 TERMO DE CIÊNCIA E DE AUTORIZAÇÃO PARA DISPONIBILIZAR VERSÕES ELETRÔNICAS DE TESES E DISSERTAÇÕES NA BIBLIOTECA DIGITAL DA UFG Na qualidade de titular dos direitos de autor, autorizo a Universidade Federal de Goiás (UFG) a disponibilizar, gratuitamente, por meio da Biblioteca Digital de Te- ses e Dissertações (BDTD/UFG), regulamentada pela Resolução CEPEC nº 832/2007, sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o documento conforme permissões assinaladas abaixo, para fins de leitura, impres- são e/ou download, a título de divulgação da produção científica brasileira, a partir desta data. 1. Identificação do material bibliográfico: [ ] Dissertação [ X ] Tese 2. Identificação da Tese ou Dissertação: Nome completo do autor: Cintia Faria da Silva Título do trabalho: Bioprospecção de bactérias à partir da comunidade endofítica ra- dicular cultivável de Aloe vera (L.) Burm. F. (Xanthorrhoeaceae) 3. Informações de acesso ao documento: 1 Concorda com a liberação total do documento [ X ] SIM [ ] NÃO Havendo concordância com a disponibilização eletrônica, torna-se imprescin- dível o envio do(s) arquivo(s) em formato digital PDF da tese ou dissertação. 2 Assinatura do(a) autor(a) Ciente e de acordo: Assinatura do(a) orientador(a)² Data: 24/10/2019 1 Neste caso o documento será embargado por até um ano a partir da data de defesa. A extensão deste prazo suscita justificativa junto à coordenação do curso. Os dados do documento não serão disponibilizados durante o período de embargo. Casos de embargo: - Solicitação de registro de patente; - Submissão de artigo em revista científica; - Publicação como capítulo de livro; - Publicação da dissertação/tese em livro. 2 A assinatura deve ser escaneada. Versão atualizada em setembro de 2017. CINTIA FARIA DA SILVA BIOPROSPECÇÃO DE BACTÉRIAS A PARTIR DA COMUNIDADE ENDOFÍTICA RADICULAR CULTIVÁVEL DE Aloe vera (L.) BURM. F. (Xanthorrhoeaceae) Orientador: Prof. Edson Luiz Souchie, PhD Co-orientador: Prof. Marcos Antônio Soares, PhD Tese apresentada à Universidade Federal de Goiás como parte das exigências do Programa de Pós-Graduação em Biotecnologia e Biodiversidade, para obtenção do título de Doctor Scientiae. Rio Verde Goiás – Brasil 2019 Ficha de identificação da obra elaborada pelo autor, através do Programa de Geração Automática do Sistema de Bibliotecas da UFG. Faria da Silva, Cintia Bioprospecção de bactérias à partir da comunidade endofítica radicular cultivável de Aloe vera (L.) Burm. F. (Xanthorrhoeaceae) [manuscrito] / Cintia Faria da Silva. - 2019. xi, 104 f.: il. Orientador: Prof. Dr. Edson Luiz Souchie; co-orientador Dr. Marcos Antônio Soares. Tese (Doutorado) - Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública (IPTSP), Programa de Pós graduação em Biotecnologia e Biodiversidade, Cidade de Goiás, 2019. Bibliografia. Inclui lista de figuras, lista de tabelas. 1. Babosa. 2. Micro-organismos. 3. Traços funcionais. 4. Promoção de crescimento vegetal. 5. Biotecnologia. I. Luiz Souchie, Edson, orient. II. Título. CDU 60 AGRADECIMENTOS A Deus, por me amparar nos momentos difíceis e refúgio nas horas de cansaço. A minha família, meus pais, Severino e Maria Aparecida, e minhas irmãs Lessi, Silvia e Nádia, pelo apoio, incentivo e paciência no decorrer desta jornada. Aos meus sobrinhos Ana Lara, Humberto e Catarina. Ao professor Dr. Edson Luiz Souchie, pela orientação, amizade, confiança e incentivo, com palavras de apoio, acreditando na minha capacidade, fazendo com que eu sempre seguisse em frente. Ao professor Dr. Marcos Antônio Soares, pela disponibilidade em minha co- orientação. As professoras Dras. Maria Andréia Corrêa Mendonça e Luciana Cristina Vitorino por toda dedicação, ensinamentos e contribuições para o meu crescimento profissional. A professora Dra. Paula Fabiane Martins, que pelo pouco tempo em que passou em Rio Verde, pode dividir seu conhecimento e paixão pela microbiologia. Aos professores Dr. Paulo Sérgio Pereira e Dra. Erika Crispim Resende pela ajuda na parte química do trabalho. Ao professor da Universidade de São Paulo, Dr. Welington Luiz Araujo e a pós doutoranda, Dra. Manuella Nóbrega Dourado Ribeiro, por me receberem de portas abertas no Laboratório de Biologia Molecular e Ecologia Microbiana para contribuição em parte das análises moleculares. A minha amiga Andréia, que conheci no início da seleção do doutorado e dividiu comigo diversos momentos, em disciplinas em outras cidades e diversas horas de conversas por telefone, meu imenso carinho. A Paula que esteve comigo desde a época do mestrado, com carinho e companheirismo e amor a microbiologia, foi parceira de viagens, disciplinas e experimentos, participando do meu aprendizado e crescimento. Aos colegas e amigos que conquistei ao longo do mestrado e doutorado, especialmente ao Thiago, Deborah, Priscila, Marília, Kelly e Paula pelo convívio, aprendizado e momentos divididos juntos, sejam eles bons ou ruins. Às colegas e amigas Ana Paula, Carine, Michelle, Daniele, Mariluza, Luciana, Elisvane, Leticia, Vanessa e Ana Cláudia, que deixaram vários momentos mais suaves, com palavras de apoio e risadas. iii Aos companheiros do Laboratório de Microbiologia Agrícola que passaram por lá e aos que chegaram a pouco tempo, Isabel, Moacir, Tenille, Juliana, Lorena Lara, Elizabeth, Tonhão, Thales, Yasmin, Matheus, Natasha, Tâmara, Leônidas, Bárbara, Ana Flávia, Kelly, Fellipe e Lidiane, pela colaboração e convívio. Em especial ao alunos de inciação científica que participaram diretamente deste trabalho Heitor, Lázara, Milene, Leonardo, Thayná e Isabelle, que me ajudaram nas diversas etapas do trabalho. Muito obrigada! Ao Programa de Pós-graduação em Biotecnologia e Biodiversidade da Rede Pró Centro - Oeste, juntamente com a Universidade Federal de Goiás e o Instituto Federal Goiano pela oportunidade de realização deste trabalho. Ao Instituto Federal Goiano, campi Rio Verde e Iporá pela estrutura disponibilizada para execução do projeto. Às agências de fomento CAPES, CNPq e FAPEG, pelo apoio financeiro. A todos que direta ou indiretamente colaboraram para a realização deste trabalho o meu sincero agradecimento! iv “A quem te pedir um peixe, dá uma vara de pescar.” Pensando bem, não só a vara de pescar, também a linhada, o anzol, a chumbada, a isca…” Cora Coralina v BIOGRAFIA DA AUTORA Cintia Faria da Silva, natural de Rio Verde – GO, filha de Severino Ferreira da Silva e Maria Aparecida Faria da Silva, graduada em Ciências – Licenciatura Plena Habilitação em Biologia pela Universidade de Rio Verde (2005), especialista em Microbiologia e Higiene de Alimentos pela Pontifícia Universidade Católica de Goiás (2009), mestra em Ciências Agrárias - Agronomia (2014) pelo Instituto Federal Goiano – Campus Rio Verde. Em agosto de 2014, ingressou no Programa de Pós-Graduação em Biotecnologia e Biodiversidade (Doutorado) da Rede Pró Centro-Oeste pela Universidade Federal de Goiás e Instituto Federal Goiano – Campus Rio Verde, concluindo em setembro de 2019. vi SUMÁRIO INDICE DE TABELAS ............................................................................................. viii ÍNDICE DE FIGURAS ................................................................................................ ix RESUMO ..................................................................................................................... 1 2. REVISÃO DE LITERATURA .................................................................................. 5 2.1. Aloe vera ............................................................................................................ 5 2.2. Aloína ................................................................................................................ 6 2.4. Micro-organismos endofíticos ............................................................................ 8 2.5. Traços funcionais ............................................................................................. 11 2.5.1. Síntese de fitormônios ................................................................................ 12 2.5.2. Solubilização de fosfatos ............................................................................ 13 2.5.3. Produção de sideróforos ............................................................................. 14 2.5.4. Controle biológico - Antibiose.................................................................... 15 3. REFERÊNCIAS BIBLIOGRÁFICAS ..................................................................... 17 3. JUSTIFICATIVA ................................................................................................... 31 4. REFERÊNCIAS BIBLIOGRÁFICAS ..................................................................... 32 OBJETIVO GERAL ................................................................................................... 34 OBJETIVOS ESPECÍFICOS ...................................................................................... 35 CAPÍTULO I .............................................................................................................. 36 Artigo - Screening of plant growth-promoting endophytic bacteria from the roots of the medicinal plant Aloe vera .................................................................................
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