Azure Biosystems™ Product Catalog
Rev 20180613 www.azurebiosystems.com • [email protected] • +1.925.307.7217 1 2 www.azurebiosystems.com • [email protected] • +1.925.307.7217 About Azure Biosystems
At Azure Biosystems, we develop easy-to-use, high-performance imaging systems and high-quality reagents for life science research. By bringing a fresh approach to instrument design, technology, and user interface, we move past incremental improvements and go straight to innovations that substantially advance what a scientist can do. And in focusing on getting the highest quality data from these instruments—low backgrounds, sensitive detection, robust quantitation—we’ve created a line of reagents that consistently delivers reproducible results and streamlines workflows.
Providing scientists around the globe with high-caliber products for life science research, Azure Biosystems’ innovations open the door to boundless scientific insights.
Learn more at azurebiosystems.com.
cSeries Imagers Sapphire Ao Absorbance Reagents & Biomolecular Imager Microplate Reader Blotting Accessories
Download our free Western Blotting Guidebook at www.azurebiosystems.com/guidebook.
Corporate Headquarters
6747 Sierra Court Phone: (925) 307-7127 Please send purchase orders to: Suite A-B (9am–4pm Pacific time) [email protected] Dublin, CA 94568 To dial from outside of the US: For product inquiries, please email USA +1 925 307 7127 [email protected] FAX: (925) 905-1816
Copyright © 2019 Azure Biosystems. All rights reserved. The Azure Biosystems logo, Azure Biosystems™, cSeries™, Sapphire™ and Radiance™ are trademarks of Azure Biosystems, Inc. More information about Azure Biosystems intellectual property assets, including patents, trademarks and copyrights, is available at www.azurebiosystems.com or by contacting us by phone or email. All other trademarks are property of their respective owners.
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Quickly Find the Solution to Wash Buffers 35 Your Western Woes 4 Azure Blot Washing Buffer 36 IMAGING 5 Azure Fluorescent Blot Washing Buffer 37 Sapphire™ Biomolecular Imager 6 Chemiluminescent Detection 39 cSeries™ Imaging Systems 10 HRP-conjugated Secondary Antibodies 40 Azure c280 12 Azure ChemiWriter ECL 41 Azure c150 and c200 13 Radiance Substrates 42 Software 14 Radiance ECL 43 Accessories 16 Radiance Q 44 REAGENTS AND CONSUMABLES 18 Radiance Plus Substrate 45 Blotting Accessories 19 Azure Flash Western Incubation Trays 20 Chemiluminescence Kit 46 Background Quenching Sheets 21 HRP Stripping Buffer 47 Blot Development Folders 22 Fluorescent Detection 49 Transfer to Membrane 23 AzureSpectra Fluorescent Secondary Antibodies 50 Pre-cut Membranes 24 AzureSpectra Labeling Kits 52 Blotting Paper for Western Blotting 25 AzureSpectra Fluorescent Western Azure Transfer Buffer 26 Blotting Kits 53 Total Protein Detection 27 AzureSpectra Fluorescent Demo Kits 54 Azure Ponceau Stain 28 AzureRed Fluorescent Protein Stain 29 MICROPLATE READER 55 Membrane Blocking 31 Ao Absorbance Microplate Reader 56 Azure Chemi Blot Blocking Buffer 32 Ao Absorbance Microplate Reader Accessories and Filters 57 Azure Protein-Free Blot Blocking Buffer 33 Azure Fluorescent Blot Blocking Buffer 34
Quickly Find the Solution to Your Western Woes
Sensitivity Lower Save Time Quantitation Sample Reagents optimized Background Products designed Products designed Reagents that are to increase limit Reagents optimized to save time. to increase available to sample. of detection. to lower background quantitative for cleaner blots. accuracy.
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Sapphire™ Biomolecular Imager
The next generation of laser-based imaging
Breakthrough image capture and analysis
The Sapphire Biomolecular Imager is a next generation laser scanning system that provides you with exceptional data quality through extremely sensitive detection, ultra high resolution and broad linear dynamic range.
This system supports long and short wavelengths of near infrared fluorescence (NIR), red/green/blue (RGB) imaging, chemiluminescent imaging, phosphor imaging as well as optical densitometry (OD) of proteins in stained gels.*
• Improved multiplex fluorescent detection • Chemiluminescence imaging, surpassing film • Higher sensitivity for lower limits of detection (femtograms) • Broad linear dynamic range for accurate quantitation • Ease of use with intuitive control software *Patent pending
FLUORESCENCE IMAGING Improve Your Quantitation • Stable fluorescent signals enable reliable quantitation of weak and strong bands • Probe for multiple proteins on a single blot, and get more results from each sample
Part Number Name Description IS1024 Sapphire Biomolecular Imager – NIR Laser excitation wavelengths at 685nm and 784nm IS1025 Sapphire Biomolecular Imager – RGB Laser excitation wavelengths at 488nm, 520nm and 658nm IS1026 Sapphire Biomolecular Imager – RGBNIR Laser excitation wavelengths at 488nm, 520 nm, 658nm and 784 nm IS1027 Sapphire Biomolecular Imager – PI Sapphire Biomolecular Imager for phosphor imaging Additional Modules IS1003 Chemiluminescence Module Add chemiluminescence detection and white light imaging to Sapphire Biomolecular Imager IS1004 Phosphor Imaging Module Add phosphor imaging to Sapphire Biomolecular Imager IS1018 Q-Module – 520 Laser Module Add 520nm laser and detector to Sapphire Biomolecular Imager – NIR
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Sapphire™ Biomolecular Imager
CHEMILUMINESCENCE IMAGING PHOSPHOR IMAGING Enhance your Detection Improve Your Flexibility • Femtogram detection of protein • Scan storage phosphor screens for filmless autoradiography • The speed and sensitivity of traditional film with 4X the • Wide dynamic range for dynamic range sensitive detection
Specifications Sapphire NIR Sapphire RGB Sapphire RGBNIR Sapphire PI
Part number IS1024 IS1025 IS1026 IS1027 Laser excitation 685, 784 488, 520, 658 488, 520, 658, 784 658 wavelengths Bit depth 16 bit 16 bit 16 bit 16 bit
Scanning area 25 cm X 25 cm 25 cm X 25 cm 25 cm X 25 cm 25 cm X 25 cm
Scanning mode Simultaneous or Simultaneous or Simultaneous or Phosphor Imaging Sequential Sequential Sequential Resolution 10 micron – 1000 micron 10 micron – 1000 micron 10 micron – 1000 micron 10 micron – 1000 micron Detectors Avalanche Photodiode Photomultiplier Tube, Photomultiplier Tube, Photomultiplier Tube Avalanche Photodiode Avalanche Photodiode Scanning speed 50 cm/s 50 cm/s 50 cm/s 50 cm/s Filters Red 725BP40, Blue 518BP22, Blue 518BP22, 390BP40 IR 832BP37 Green 565BP24, Green 565BP24, Red 710BP40 Red 710BP40, IR 832BP37 Dimensions 75 cm X 45 cm X 70 cm 75 cm X 45 cm X 70 cm 75 cm X 45 cm X 70 cm 75 cm X 45 cm X 70 cm (W X H X D) Weight 140 lbs 140 lbs 140 lbs 140 lbs Power requirements 100 – 240 VAC ± 10%, 100 – 240 VAC ± 10%, 100 – 240 VAC ± 10%, 100 – 240 VAC ± 10%, 50/60 Hz 50/60 Hz 50/60 Hz 50/60 Hz Computer Windows 10 laptop or Windows 10 laptop or Windows 10 laptop or Windows 10 laptop or Windows Pro desktop PC Windows Pro desktop PC Windows Pro desktop PC Windows Pro desktop PC and 4K monitor and 4K monitor and 4K monitor and 4K monitor Q-Module Optional N/A N/A N/A Laser excitation 520 wavelength Filters Green 565BP24 Chemiluminescent Optional Optional Optional Optional Upgrade Imaging area, 16 cm X 13 cm 16 cm X 13 cm 16 cm X 13 cm 16 cm X 13 cm chemiluminescent Bit depth 16 bit 16 bit 16 bit 16 bit Resolution 2688 X 2200 2688 X 2200 2688 X 2200 2688 X 2200
Imaging time 0-60 minutes 0-60 minutes 0-60 minutes 0-60 minutes Visible imaging Yes Yes Yes Yes Phosphor Imaging Optional Optional Optional N/A Module Sample types Storage phosphor screen Storage phosphor screen Storage phosphor screen (imaging plate) (imaging plate) (imaging plate)
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Sapphire™ Biomolecular Imager
Dragonfly Corn 488nm – 658nm – 784nm – 10micron 488nm – 658nm – 10micron
2D Gel Image Dragonfly (Zoom) Untreated HeLa lysate was labeled with Cy3. Treated HeLa 488nm – 658nm – 784nm – 10micron lysate was labeled with Cy5. Samples were simultaneously separated using IEF in the first dimension and SDS-PAGE in the second dimension.
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Sapphire™ Biomolecular Imager
Grasshopper (Zoom) Sea Star 488nm – 658nm – 784nm – 10micron 488nm – 520nm – 658nm – 784nm – 10micron
Multiplex Fluorescence Mouse Heart (Black & White) Up to 4 different fluorescent probes can be used and imaged 658nm – 10micron at once.
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c600, c500, c400 and c300
Big performance, small footprint, incomparable value
Great science starts with high-quality data, and when it comes to imaging gels, blots, plates, and even intact tissues and small animal models, high-quality data starts with the cSeries.
Leveraging Azure Biosystems’ deep expertise in imaging system engineering, the cSeries delivers best-in-class sensitivity, dynamic range, and signal-to-noise ratio in an easy-to-use, compact instrument.
• Get high-quality data from an expertly-engineered system • Perform a wide range of imaging applications with a single, versatile instrument • Choose a system for today’s needs and upgrade as your detection methodologies change • Seamlessly integrate the cSeries into your studies with easy-to-use image acquisition and analysis workflows • Save space with our compact design • Rest easy with a dedicated team ready to answer questions, troubleshoot, and provide on-site support
IR FLUORESCENCE, VISIBLE FLUORESCENCE, UV FLUORESCENCE, CHEMILUMINESCENCE VISIBLE IMAGING and BLUE LIGHT EXCITATION Gels | Blots | Plates | Tissues | Small Animal Models | Plants
In-gel Fluorescence Stain-Free™ Gels Western Blot with Cy3 Escherichia coli with GFP and TAMRA and Cy5 Dyes
Chemiluminescent Mouse brain sections Mouse with RFP-labeled Arabidopsis thaliana Western blot subcutaneous tumor
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Choose Your System
c600 c500 c400 c300 The All-around The Ultimate IR The Visible The Darkroom Application Ace Imaging System Fluorescence Virtuoso Eliminator
NIR RGB CHEMI NIR CHEMI RGB CHEMI CHEMI
UV WHITE BLUE UV WHITE BLUE UV WHITE BLUE UV WHITE BLUE LIGHT LIGHT LIGHT LIGHT LIGHT LIGHT LIGHT LIGHT
Upgradable to c400, Upgradable to c600 Upgradable to c600 c500, and c600
Specifications c600 c500 c400 c300
Part number AC6001 AC5001 AC4001 AC3001
Excitation sources Dual wavelength UV Dual wavelength UV Dual wavelength UV Dual wavelength UV transilluminator at transilluminator at transilluminator at transilluminator at 302 nm and 365 nm; EPI 302 nm and 365 nm; EPI 302 nm and 365 nm; EPI 302 nm and 365 nm; EPI blue LEDs at 470 nm; EPI blue LEDs at 470 nm; blue LEDs at 470 nm; EPI blue LEDs at 470 nm LEDs at 628 nm, 530 nm laser diodes at 660 nm LEDs at 628 nm, 530 nm and 457 nm; laser diodes and 785 nm and 457 nm at 660 nm and 785 nm
Filters 7 position motorized 7 position motorized 7 position motorized 7 position motorized filter wheel with 6 filters filter wheel with 3 filters filter wheel with 4 filters filter wheel with 1 filters standard standard standard standard
Detector 8.3 MP CCD camera 8.3 MP CCD camera 8.3 MP CCD camera 8.3 MP CCD camera cooled -50°C below cooled -50°C below cooled -50°C below cooled -50°C below ambient, f/0.95 motorized ambient, f/0.95 motorized ambient, f/0.95 motorized ambient, f/0.95 motorized fixed lens fixed lens fixed lens fixed lens
Bit depth 16-bit 16-bit 16-bit 16-bit
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Azure c280
Powerful and Economical Chemiluminescent Imaging
Application Flexibility
• Picogram Detection of Proteins with Chemiluminescent Westerns—The Azure cSeries provides accurate and fast chemiluminescent detection, as well as the sensitivity, dynamic range, and linearity needed for quantitative blot analysis. Get the best picture every time, and stop wasting money in the darkroom.
• “Safe” Dye Detection—An alternative to ethidium bromide, less- harmful ‘Safe’ dyes can be imaged with the EPI Blue LEDs standard in the system.
• Protein Analysis—Protein gels stained with Coomassie blue or silver stain can easily be imaged using the visible light application.
• Fluorescent Detection for UV—With a dual wavelength 302nm and 365nm UV transilluminator, images of ethidium bromide-stained DNA gels or Stain-free gels can be captured. An interlock switch prevents the accidental exposure of UV.
Chemiluminescence Blue Light Imaging White Light Imaging UV Imaging SYBR® Safe, SYBR® Gold, Coomassie Blue, Silver Stain Ethidium Bromide SYBR® Green
Specifications c280
Part number AC2801
Excitation sources Dual wavelength UV transilluminator at 302 and 365 nm, Orange Tray, White Blot Tray, EPI blue LEDs (470 nm) for gel applications
Filters 7 position motorized filter wheel with 1 filter standard
Detector 6.1 MP CCD camera cooled -50°C below ambient, motorized fixed lens
Bit depth 16-bit
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Azure c150 and c200
Gel Imaging Workstations
Application Flexibility
• “Safe” Dye Detection—An alternative to ethidium bromide, less- harmful ‘Safe’ dyes can be imaged with the EPI Blue LEDs standard in the system.
• Protein Analysis—Protein gels stained with Coomassie blue or silver stain can easily be imaged using the visible light application.
• Protein Analysis—Protein gels stained with Coomassie blue or silver stain can easily be imaged using the visible light application.
• DNA Detection with Ethidium Bromide—With the a dual- wavelength 302nm and 365nm UV transilluminator, images of ethidium bromide-stained DNA gels can be captured in a fraction of a second. An interlock switch prevents accidental exposure to UV. For band excision, the switch can be overridden with the press of a button, and the UV transilluminator can be pulled out.
Blue Light Imaging White Light Imaging UV Imaging SYBR® Safe, SYBR® Gold, Coomassie Blue, Silver Stain Ethidium Bromide SYBR® Green
Specifications c200 c150
Part number AC2001 AC1501
Excitation sources Dual wavelength UV transilluminator at 302 and Dual wavelength UV transilluminator at 302 and 365 nm, Orange Tray, Black Tray, EPI blue LEDs 365 nm, Orange Tray, Black Tray, EPI blue LEDs (470 nm) for gel applications (470 nm) for gel applications
Filters 7 position filter wheel with 1 filter standard 3 position filter slider with 1 filter standard
Detector 5.4 MP image resolution, f/1.8 fixed lens 5.4 MP image resolution, f/1.8 fixed lens
Bit depth 16-bit 16-bit
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Software
AzureSpot Analysis Software
Advanced Analysis for 1D Gels and Blots
AzureSpot helps you interpret your data easily and accurately. Analyze your gels and blots in either manual or automatic modes.
AzureSpot can automatically detect lanes and bands, even on distorted gels, and apply your selected method of background correction.
Visualize intensity plots by lane (sample) and channel (probe) to get detailed information about your proteins of interest.
The integrated tutorial helps you get the most information out of your analyses. Generate a PDF report of your experiment when you are done. You can also export your data in many formats to other applications (e.g. Excel) for further analysis.
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Software
AzureSpot can Product Features • Perform 1D densitometry • Integrated tutorial • Automatically detect lanes and bands • PDF report generator • Correct for background • Lane creation tools • Calculate molecular weights • Molecular size/pI calibration • Quantify fluorescent signals • Quantity calibration toolbox • Annotate your results • Module for easy shape selection/deselection • Visualize multiplex analyses • Wide range of data fields to display • Count colonies in measurements • Normalize signals on blots • Annotation tools for comments and highlighting of image • Analyze arrays and plate-based assays • Various normalization options including • Export reports housekeeping protein and total protein normalization
Hardware and Software Requirements • Operating Systems: Windows 10, Windows 8, Windows 7 and Windows Vista • Processor 1.4 GHz • Memory Minimum 256MB, recommended 512MB • Free Hard Disk Space Recommended 5GB
Compatible with both the cSeries and the Sapphire Biomolecular Imager.
Part Number Name Description AC1061 AzureSpot Single AzureSpot license AC1062 AzureSpot N1 Unlimited AzureSpot Network license with 1 user at any time AC1063 AzureSpot N3 Unlimited AzureSpot Network license with up to 3 simultaneous users at any time AC1064 AzureSpot N5 Unlimited AzureSpot Network license with up to 5 simultaneous users at any time AC1065 AzureSpot N20 Unlimited AzureSpot Network license with up to 20 simultaneous users at any time
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Accessories
Thermal Printer
Digital thermal grayscale USB printer.
Part Number Name Description AC1011 Thermal Printer Includes USB cable, driver, and one roll of thermal paper
Thermal Paper
One box (4 rolls) High Density Thermal Paper.
Part Number Name Description AC1012 Thermal Paper Each roll measures 110mm x 21m
UV Safety Glasses/Goggles
For use with UV light or Blue light.
Part Number Name Description AC1022 UV Safety Recommended accessory for Glasses/Goggles band excision
Screens
Part Number Name Description AC1024 Gel Cutting Screen UV transmitting plastic that protects the transilluminator glass during band excision from gels AC1025 Blue Light For trans illumination of blue light. Conversion Screen Converts UV light to blue light
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Accessories
Trays
Part Number Name Description AC1023 Gel Carrying Tray UV Transmitting Tray with raised edges designed for transporting your sample into the imager (size 9x7 inches) AC1016 Orange Tray For setting under Coomassie blue, silver stained, and other colorimetric gels during imaging AC1017 Black Tray Black tray designed for optimal blot performance
Computer/Tablet Accessories
Part Number Name Description AC1013 USB Keyboard USB wireless keyboard compatible with cSeries imager AC1014 USB Mouse USB wireless mouse compatible with cSeries imager AC1015 Stylus Pointer tip for touch screen compatible with cSeries imager
Sapphire Imaging Accessories
Part Number Name Description IS1010 Sapphire Mat Designed for flattening membranes against the glass surface IS1011 Phosphor Screen Multipurpose screen for ionizing radiation (20 x 25 cm) IS1012 Cassette for Cassette for the multipurpose screen Phosphor Screen (20 x 25 cm)
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection REAGENTS AND CONSUMABLES
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Blotting Accessories
Incubation Trays
For staining and washing gels and membranes These specially designed incubation trays are ideal for staining and washing electrophoresis gels and membranes. Available in a range of sizes in both clear and opaque.
Features • Lidded —to protect experiments from dust or debris • Non-binding—surfaces treated with silicone to prevent antibody loss • Convenient—multiple sizes available for different applications • Opaque or clear—opaque trays are ideal for dealing with light sensitive fluorescent blots
Part Number Name Description Size AC2120 Incubation Tray – 7x5cm For staining and washing light sensitive gels 5 trays (opaque black top and bottom) and membranes AC2121 Incubation Tray – 9x6cm For staining and washing light sensitive gels 4 trays (opaque black top and bottom) and membranes AC2122 Incubation Tray – 11x9cm For staining and washing light sensitive gels 3 trays (opaque black top and bottom) and membranes AC2123 Incubation Tray – 15.5x10.5cm For staining and washing light sensitive gels 2 trays (opaque black top and bottom) and membranes AC2150 Incubation Tray – 7x5cm (clear) For staining and washing gels and 5 trays membranes AC2151 Incubation Tray – 9x6cm (clear) For staining and washing gels and 4 trays membranes AC2152 Incubation Tray – 11x9cm (clear) For staining and washing gels and 3 trays membranes AC2153 Incubation Tray – 15.5x10.5cm (clear) For staining and washing gels and 2 trays membranes
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Blotting Accessories
Background Quenching Sheets
Background quenching sheets for use with fluorescent or chemiluminescent samples Use these quenching sheets under your blot when imaging chemiluminescent or fluorescent Westerns. The sheets reduce background due to contamination or auto-fluorescence, improving the quality of your image.
Part Number Name Description Size AC2144 Quenching Sheets – 9x12.5cm Background quenching sheets for use with 10 sheets fluorescent or chemiluminescent samples
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Blotting Accessories
Blot Development Folders
Transparent plastic sheets to hold blots for imaging A wrinkle-free alternative to plastic wrap, these transparent plastic folders hold your blot during handling and imaging and help maximize the signal longevity of Radiance and Radiance Plus Substrates.
Part Number Name Description Size AC2126 Blot Development Folders Transparent plastic sheets to hold blots for 100 sheets imaging – alternative to plastic wraps
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection
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Transfer to Membrane
Pre-cut Membranes
Pre-cut PVDF membranes for a variety of Western blotting applications save you time in the lab.
Features • Performance—quality-controlled for background-free Westerns • Convenience—save time, and avoid concerns about membrane contamination • Flexibility—available in PVDF and nitrocellulose, choose the right membrane for your application
PVDF pre-cut membranes for Western blotting applications Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. Available in three different pre-cut sizes.
Nitrocelluose pre-cut membranes for Western blotting applications Low-fluorescence Nitrocellulose membranes are available in both 0.45 µm and 0.22 µm pore sizes to accommodate larger and smaller proteins, respectively.
PVDF Nitrocellulose • High binding capacity is great for • Low molecular weight proteins low expressing proteins • Typically used for chemi • Typically used for fluorescence • Not ideal for stripping • High protein retention for stripping and reprobing
Part Number Name Description Size AC2105 Low Fluorescence Western Membrane Pre-cut 9x7 cm 10 sheets (PVDF) 0.45 µm AC2108 Low Fluorescence Western Membrane Pre-cut 10x15 cm 10 sheets (PVDF) 0.45 µm AC2109 Low Fluorescence Western Membrane Pre-cut 13x18 cm vertical gels 10 sheets (PVDF) 0.45 µm AC2106 Nitrocellulose Transfer Membrane Pre-cut 8x10 cm 10 sheets 0.45 µm AC2107 Nitrocellulose Transfer Membrane Pre-cut 8x10 cm 10 sheets 0.22 µm
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Transfer to Membrane
Blotting Paper for Western Blotting
Pre-cut blotting paper for Western blot transfer Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”.
Blotting papers are cut to completely cover the gels and membranes. • For 8x10cm gels—blotting papers are 9x13cm • For 7x9cm gels—blotting papers are 8x10cm
Part Number Name Description Size AC2110 Blotting Paper for Western Blotting Pre-cut blotting paper for Western blot 60 sheets transfer—7x9 cm vertical gels AC2111 Blotting Paper for Western Blotting Pre-cut blotting paper for Western blot 60 sheets transfer—8x10 cm vertical gels
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Transfer to Membrane
Azure Transfer Buffer
Enhanced protein transfer for improved sensitivity Azure’s proprietary transfer buffer provides improved detection of low- abundance and post-translationally modified proteins. High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.
Features • High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low- abundance and post-translationally modified proteins • Efficient—enhanced transfer efficiency for proteins of all sizes • Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers • Convenient—use your existing wet transfer electrophoresis apparatus
CEA 180 kDa GAPDH 37 kDa 20 10 5 2.5 ng 100 50 25 12 ng Azure Transfer Buffer
Towbin Buffer Azure Transfer Buffer – 15 min transfer
0 100 200 300 400 500 600 Transfer Time (minutes)
Towbin Buffer – 60 min transfer Shorter run times. Azure Transfer Buffer achieves complete protein transfer in less Complete transfer in less than 20 minutes! Transfer of CEA than 20 minutes. (high MW) and GAPDH (low MW) proteins was compared using Azure Transfer Buffer and Towbin transfer buffers. Following Western blot analysis, the data show equivalent protein transfer in 15 minutes with Azure Transfer Buffer vs. 60 minutes with Towbin buffer.
Part Number Name Description Size AC2127 Azure Transfer Buffer 50x concentrated solution sufficient for 500 ml 50-100 blots
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection
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Total Protein Detection
Azure Ponceau Stain
Quick total protein stain for membranes Azure Ponceau detects total protein on nitrocellulose and PVDF membranes, allowing you to check the quality of protein transfer before proceeding with Western blotting. Use Azure Ponceau Stain to quickly make sure that protein transfer as been even across the entire blot, and that no bubbles or other transfer artifacts are present. The staining is reversible and after destaining, membranes can immediately be used for Western blotting.
Features • Quick—protein staining is complete in 5 minutes or less • Reversible—easily destain membrane with water • Compatible—after destaining, PVDF and nitrocellulose membranes are ready for Western blotting
Part Number Name Description Size AC2125 Azure Ponceau Stain Visible reversible total protein stain for 500 ml proteins transferred to membrane— sufficient for staining 50 blots 9x7 cm
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Total Protein Detection
AzureRed Fluorescent Protein Stain
Quantitative fluorescent total protein stain
Features • Sensitive —detect less than a nanogram of protein per spot • Convenient—simple 30 minute protocol for blots or 3 hour protocol for gels • Flexible—stain gels or membranes • Reversible—destain for downstream Western blotting or mass spectrometry
A membrane was stained with • No speckling—clean background and no speckling for better data AzureRed and probed for three • Safe—biodegradable and contains no heavy metals for increased proteins. The resulting fluorescent Western blot was scanned on a safety and simple disposal Sapphire Biomolecular Imager to detect all three proteins and the total • Compatible—image stained blots or gels with laser or CCD imaging protein stain simultaneously. systems. Multiplex stained membranes with fluorescent Western blots for simultaneous detection of total protein and proteins of interest.
Total protein normalization Use AzureRed as a fluorescent total protein normalization stain. The 30 minute protocol produces superior linearity, sensitivity and reproducibility and is compatible with multiplex fluorescent Western blotting. 1.2 pg 20 ng Stain 1D and 2D gels AzureRed stains 1D and 2D gels in less than 3 hours, with high sensitivity, low background, and no speckling. R2 = 0.9958
BSA
AzureRed – 3 hour protocol Log (integrated intensity) (integrated Log
Log [Transferrin (pg)] Coomassie blue stain – overnight protocol Quantitative staining with AzureRed. Gel High sensitivity, fast protocol. AzureRed outperforms both containing a serial dilution of transferrin was stained Coomassie blue and SYPRO® Ruby in time and sensitivity. Gels with AzureRed and imaged with the 520nm channel were stained with AzureRed, SYPRO Ruby, or Coomassie blue of the Sapphire Biomolecular Imager. Less than 2pg stain, according to the manufacturers’ protocols and imaged on of protein per band can be detected, and the signal a CCD imager. is linear over more than 4 orders of magnitude.
Part Number Name Description Size AC2124 AzureRed Fluorescent Protein Stain Fluorescent total protein stain for 1D and 2D 5ml gels – sufficient for 20 minigels
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection
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Membrane Blocking
Azure Chemi Blot Blocking Buffer
Ready-to-use block that enhances chemiluminescent signal Azure Chemi Blot Blocking Buffer was formulated specifically for chemiluminescent Western blots to enhance signal and lower background. The 1X formula comes ready-to-use with no mixing or dilutions required.
Azure Chemi Blot Milk Blocking Buffer
Part Number Name Description Size AC2148 Azure Chemi Blot Blocking Buffer Provided as a 1x Ready-to-Use Solution 500mL
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Membrane Blocking
Azure Protein-Free Blot Blocking Buffer
Protein-Free Solution for Fluorescent Immunoblots Azure Protein-Free Blot Blocking Buffer is a protein-free blocking solution optimized for fluorescent Western blots. It also serves as an excellent blocking reagent for chemiluminescent Western blotting.
Azure Protein-Free Blot Blocking Buffer can reduce background when used with primary antibodies that have a high degree of cross-reactivity with protein blockers such as BSA, casein or milk protein.
Part Number Name Description Size AC2112 Azure Protein-Free Blot Blocking Buffer Provided as a 5x solution 200mL
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Membrane Blocking
Azure Fluorescent Blot Blocking Buffer
Ready-to-use block that stabilizes fluorescent signal Azure Fluorescent Blot Blocking Buffer is optimized for use with Azure’s fluorescent Western blotting systems. The 1x solution is ready-to-use and formulated to lower background noise and stabilize fluorescent signal.
A B
Dramatic effect of blocking buffer on background and signal- to-noise ratio. Duplicate dot blots were processed and imaged identically except for the blocking step which was either 5% nonfat dry milk in TBST (A) or Azure Protein-Free Blot Blocking Buffer (B).
Part Number Name Description Size AC2190 Azure Fluorescent Blot Blocking Buffer Provided as a 1x Ready-to-Use Solution 500mL
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection
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Wash Buffers
Azure Blot Washing Buffer
One wash for any Western blotting system Azure Blot Washing Buffer can be used with any Western blotting system, including all chemiluminescent and visible fluorescent Western blotting kits. Provided as a 10x concentrate.
Good wash Insufficient wash
Part Number Name Description Size AC2113 Azure Blot Washing Buffer Provided as a 10x concentrated solution 500mL
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Wash Buffers
Azure Fluorescent Blot Washing Buffer
Washing buffer optimized for use with fluorescent secondary antibodies Optimized for use with both visible and near-infrared fluorescent Western blots, Azure’s Fluorescent Blot Washing Buffer is specially formulated to reduce non-specific binding of fluorescent secondary antibodies to produce clean blots with high signal-to-noise ratios.
Insufficient Washing Azure Fluorescent Blot Washing Buffer
Part Number Name Description Size AC2145 Azure Fluorescent Blot Washing Buffer Provided as a 10x solution 500mL
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection
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Chemiluminescent Detection
HRP-conjugated Secondary Antibodies
For chemiluminescent immunodetection Azure’s high-quality HRP-conjugated secondary antibodies offer unparalleled sensitivity and performance for immunoblotting and ELISA applications. These secondary HRP conjugated antibodies are optimized to work with Azure’s Radiance and Radiance Plus protein detection systems.
Part Number Name Size AC2114 Goat-anti-rabbit HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns AC2115 Goat-anti-mouse HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns AC2116 Goat-anti-human HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns AC2117 Goat-anti-chicken HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns AC2118 Goat-anti-rat HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns AC2119 Goat-anti-guinea pig HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns AC2149 Donkey-anti-goat HRP HRP conjugated secondary antibody for 500 μl secondary antibody chemiluminescent Westerns
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Chemiluminescent Detection
Azure ChemiWriter ECL
Chemiluminescent pen for blot annotation A chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added.
Tips • The ChemiWriter ink contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the blot. • There are two heads to the pen, a fine point tip and a thicker chisel point tip. We recommend using the fine point tip to annotate the blot and mark protein ladders for detection. • The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal. • For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol. • Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature. The Azure ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least 3 months. • The ChemiWriter should be stored tightly closed at room temperature.
Part Number Name Description Size AC2146 Azure ChemiWriter ECL Chemiluminescent pen for blot annotation 1 pen
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Chemiluminescent Detection
Radiance Substrates
Specialized chemiluminescent HRP substrates for CCD imaging Radiance chemiluminescent horseradish peroxidase (HRP) substrates are specially formulated for CCD imaging and to provide excellent performance with Azure’s cSeries imaging systems. Radiance substrates produce strong, long-lasting signal that is linear with respect to protein load.
Which substrate is right for you?
Radiance ECL Radiance Q Radiance Plus
• Economical • Higher linear dynamic range • Ultra-high sensitivity for low • Linear signal for quantitative for more reliable quantitation abundance proteins Westerns • Increased sensitivity • Extended signal longevity
µg HeLa Radiance Radiance Radiance Lysate ECL Q Plus
10
5
2.5
1.25
0.625
0.313
0.156
0.078
A serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the Radiance Substrate indicated.
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Chemiluminescent Detection
Radiance ECL Substrate
Chemiluminescent HRP substrate Radiance ECL is an enhanced chemiluminescent substrate specially developed for routine Western blotting. The strong signal and low background produces cleaner blots, allowing for more meaningful quantitative comparisons.
Features HeLa Lysate • Economical – well-suited for routine 10 µg Western blotting • Linear signal for more meaningful Radiance ECL quantitative comparisons • Long lasting signal SS-Pico
Pierce ECL
Substrate Decay Radiance ECL Signal Linearity 1.00
0.80
0.60
0.40 • Radiance ECL
Relative to T=0 Relative SuperSignal Pico 0.20 • • Peirce ECL Signal Intensity 0.00 0 20 40 60 Time (Min) µg HeLa Lysate The blots pictured to the right were imaged at regular intervals up to sixty minutes, and A 1:1 serial dilution of HeLa lysate was spotted on a membrane the signal of the second spot is plotted above. and identical dot blots were probed for GAPDH and detected Radiance ECL shows significantly less signal using the substrate indicated. The linearity of the Radiance ECL decay than other substrates. signal is shown on the bottom.
Part Number Name Name Size AC2204 Radiance ECL Enhanced Chemiluminescent HRP Substrate 500mL
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Chemiluminescent Detection
Radiance Q Substrate
Chemiluminescent substrate for quantitative chemiluminescent Westerns Radiance chemiluminescent substrate is specially designed for sensitive detection on CCD based systems. It provides the fastest, most sensitive, most quantitative chemiluminescent Western blots.
Radiance chemiluminescent substrate is specially formulated for CCD imaging. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.
Radiance
Amersham Amersham SuperSignal Radiance ECL ECL Plus West Dura
Low background for broad useful linear dynamic range. Extremely low background with Radiance. Signal that endures. Radiance produces Replicate Western blots were developed using the most stable chemiluminescent signal. Radiance or one of three other chemiluminescent Blots detected using Radiance or one of substrates. After a simultaneous 20 minute exposure three other chemiluminescent substrates to the same piece of film, Radiance displays the were re-imaged at several times over a best combination of sensitivity and signal with low 10 hour period. The intensity of one band background. All display parameters are identical is plotted. 60 minutes after substrate across all images shown in this figure. incubation, Radiance retains 70% of its initial signal strength, while the competition decays to 5% or less. Enjoy more flexibility in imaging blots, knowing the signal will not decay substantially over several hours. Also, Long-lasting signal. Radiance allows long exposures can be conducted if needed blots to be imaged several hours after to detect very low abundance bands. substrate incubation. Blots can be re- imaged to obtain the perfect exposure, without worrying about signal decay. A 5 min 60 min blot was imaged with 2 min exposures 2 min exposure after 2 min exposure after at 5 min and 60 min after substrate substrate incubation substrate incubation incubation.
Part Number Name Description Size AC2101 Radiance Q HRP Substrate for CCD Imaging – contains 150ml substrate for 1500 cm2 membrane
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Chemiluminescent Detection
Radiance Plus Substrate
Chemiluminescent substrate for the most sensitive Western blotting experiments Radiance Plus is designed for sensitive detection and low antibody concentrations. With low background and a strong, long-lasting chemiluminescent and chemifluorescent signal, Radiance Plus is the best choice for quantifying low-abundance bands.
Radiance Plus is the most sensitive HRP substrate available from Azure for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.
Features 64 32 16 8 4 2 1 0.5 pg • Detect low-abundance proteins • Low background for high signal-to-noise ratio
• Long-lasting signal The highest sensitivity. A slot blot containing serial dilutions of an HRP-conjugated antibody was incubated with Radiance Plus and detected with a 2-minute exposure time.
Signal:Noise ratio Time after substrate incubation Supersignal West 5 min 30 min 3 hr 8 hr Radiance Plus Femto
625 pg
312 pg 35.8 17.1 7.4 3.5
Low background for better band quantification. 156 pg Radiance Plus provides sensitive detection combined with extremely low background. Duplicate Western blots were 8 sec 8 sec 32 sec 128 sec conducted to analyze samples of plasma, diluted either 1:100 (left lanes) or 1:600 (right lanes) in PBS. The blots Exposure length were probed with a goat antisera primary antibody, and Image blots hours after substrate incubation. A slot blot an HRP-conjugated secondary antibody. After detection containing a serial dilution of an HRP-conjugated antibody was ® with either Radiance Plus or SuperSignal West Femto incubated with Radiance Plus and imaged at the times indicated. (Thermo Scientific), the blots were imaged for 8.3 minutes. After 8 hours, the bands are still easily detected with only a The signal-to-noise ratio is shown for each band. 2-minute exposure.
Part Number Name Description Size AC2103 Radiance Plus Femtogram-sensitivity HRP Substrate – 150ml contains substrate for 1500 cm2 membrane
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Chemiluminescent Detection
Azure Flash Western Chemiluminescence Kit
Run an entire Western Blot from gel through imaging in just 2 hours The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. Combine with Azure Transfer Buffer (AC2127) to cut wet transfer time down to 15- to 30-minutes for a total assay time of two hours from gel to imaging.
Features Primary and HRP-Conjugate • Fast protocol with few Buffer C Buffer E hands-on steps Block Wash • Antibody stabilization for Flash Western enhanced chemi signal Detection • Low background and Gel Transfer increased signal-to-noise Traditional ratios Block Wash 1 Wash 2 • Increased detection Primary Secondary sensitivity and linear 0 50 100 150 200 250 300 350 dynamic range Time (minutes) Time savings. Note: Flash Western graph includes use of Azure Transfer Buffer in light blue.
Sample Azure Flash Traditional Antibody Rapid protocol with low background and high sensitivity. Type Western (1 hr) Western (6 hrs) Replicate Western blots were performed using the Azure Flash Untreated Western Chemiluminescence Kit and a traditional Western blot STAT-3 HeLa protocol. Each blot shows a dilution series of the same sample. The Azure Flash Western Kit produced more sensitive detection than pSTAT-3 IFNa HeLa the traditional protocol with over 90% of antibodies (while STAT-3 and pSTAT-3 demonstrate the improved data quality possible with Untreated the Azure Flash Western Kit, the HSP60 antibody is an example of HSP60 HeLa an antibody with similar performance).
Part Number Name Size AC2220 Azure Flash Western Chemiluminescence Kit, Rabbit 1 Kit AC2221 Azure Flash Western Chemiluminescence Kit, Mouse 1 Kit
Each Flash Western Chemiluminescence Kit contains enough materials to perform ten chemiluminescent Western blots and includes PVDF membranes, Blocking Buffer, Flash HRP-conjugate, Flash Wash Buffer, and Radiance ECL Substrate.
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Chemiluminescent Detection
HRP Stripping Buffer
Stripping Buffer for chemiluminescent Western blots Uniformly removes antibodies from developed HRP Western blots so the blot can be reprobed with another set of antibodies.
1 2 3 4 1 2 3 4
Phospho-STAT1 STAT 1
Blot was probed for phospho-STAT1 then stripped and reprobed for STAT1. Lanes: Ladder, 1) 10 μg untreated HeLa lysate, 2) 10 μg IFNα-treated, 3) 20 μg untreated, 4) 20 μg IFNα-treated.
Part Number Name Size AC2154 HRP Stripping Buffer Provided as a 1x Ready-to-Use Solution 500mL
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Blotting Accessories
Transfer to Membrane
Total Protein Detection
Membrane Blocking
Wash Buffers
Chemiluminescent Detection
Fluorescent Detection
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Fluorescent Detection
AzureSpectra Fluorescent Secondary Antibodies
Visible and Near-IR Fluorescently Labeled Secondary Antibodies Azure offers secondary antibodies labeled with fluorophores that emit light in visible and near-infrared wavelengths. AzureSpectra 490, 550, 650, 700 and 800 secondary antibodies offer unparalleled sensitivity and performance for immunoblotting applications when used in conjunction with Azure’s Western blotting systems.
RGB
n e n e Abs rbence ntensit
AzureSpectra 490 0 300 3 0 00 0 00 0 00 0 00 0 00 0 a elength (n ) anti-Transferrin (directly labeled) AzureSpectra 490 AzureSpectra 550 goat anti-rat, Tubulin AzureSpectra 700 n e 3 n e n e n e goat anti-rabbit, beta-Actin AzureSpectra 800 goat anti-chicken, GAPDH Abs rbence ntensit Abs rbence ntensit
0 300 3 0 00 0 00 0 00 0 00 0 00 0 0 300 3 0 00 0 00 0 00 0 00 0 00 0 Excitation Emission a elength (n ) a elength (n ) max. (nm) max. (nm) AzureSpectra 550 AzureSpectra 650 l Dye-490 491 515
l Dye-550 551 565 NIR l Dye-650 653 672
l Dye-IR7000 692 709 692 nm ex 783 nm ex 709 nm em 800 nm em l Dye-IR800 783 800 Absorbence/Intensity Absorbence/Intensity
250 300 350 400 450 500 550 600 650 700 750 800 850 250 300 350 400 450 500 550 600 650 700 750 800 850 Wavelength (nm) Wavelength (nm)
AzureSpectra 700 AzureSpectra 800
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Fluorescent Detection
Part Number Name Size AC2206 AzureSpectra 490 Goat-anti-rabbit secondary antibody 250mg AC2207 AzureSpectra 490 Goat-anti-mouse secondary antibody 250mg AC2208 AzureSpectra 490 Goat-anti-human secondary antibody 250mg AC2209 AzureSpectra 490 Goat-anti-chicken secondary antibody 250mg AC2210 AzureSpectra 490 Goat-anti-rat secondary antibody 250mg AC2211 AzureSpectra 490 Goat-anti-guinea pig secondary antibody 250mg AC2217 AzureSpectra 490 Donkey-anti-goat secondary antibody 250mg AC2158 AzureSpectra 550 Goat-anti-rabbit secondary antibody 250mg AC2159 AzureSpectra 550 Goat-anti-mouse secondary antibody 250mg AC2160 AzureSpectra 550 Goat-anti-human secondary antibody 250mg AC2161 AzureSpectra 550 Goat-anti-chicken secondary antibody 250mg AC2162 AzureSpectra 550 Goat-anti-rat secondary antibody 250mg AC2163 AzureSpectra 550 Goat-anti-guinea pig secondary antibody 250mg AC2164 AzureSpectra 550 Donkey-anti-goat secondary antibody 250mg AC2165 AzureSpectra 650 Goat-anti-rabbit secondary antibody 250mg AC2166 AzureSpectra 650 Goat-anti-mouse secondary antibody 250mg AC2167 AzureSpectra 650 Goat-anti-human secondary antibody 250mg AC2168 AzureSpectra 650 Goat-anti-chicken secondary antibody 250mg AC2169 AzureSpectra 650 Goat-anti-rat secondary antibody 250mg AC2170 AzureSpectra 650 Goat-anti-guinea pig secondary antibody 250mg AC2171 AzureSpectra 650 Donkey-anti-goat secondary antibody 250mg AC2128 AzureSpectra 700 Goat-anti-rabbit secondary antibody 250mg AC2129 AzureSpectra 700 Goat-anti-mouse secondary antibody 250mg AC2130 AzureSpectra 700 Goat-anti-human secondary antibody 250mg AC2131 AzureSpectra 700 Goat-anti-chicken secondary antibody 250mg AC2132 AzureSpectra 700 Goat-anti-rat secondary antibody 250mg AC2133 AzureSpectra 700 Goat-anti-guinea pig secondary antibody 250mg AC2156 AzureSpectra 700 Donkey-anti-goat secondary antibody 250mg AC2134 AzureSpectra 800 Goat-anti-rabbit secondary antibody 250mg AC2135 AzureSpectra 800 Goat-anti-mouse secondary antibody 250mg AC2136 AzureSpectra 800 Goat-anti-human secondary antibody 250mg AC2137 AzureSpectra 800 Goat-anti-chicken secondary antibody 250mg AC2138 AzureSpectra 800 Goat-anti-rat secondary antibody 250mg AC2139 AzureSpectra 800 Goat-anti-guinea pig secondary antibody 250mg AC2157 AzureSpectra 800 Donkey-anti-goat secondary antibody 250mg
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Fluorescent Detection
AzureSpectra Labeling Kits
Fast and simple antibody labeling • Easy and fast—The fluorescent antibody labeling kit provides the materials necessary to fluorescently label the antibody of your choice in less than 1 hour • Label primary antibodies—use antibodies that your lab has already validated and optimized. Labeled primary antibodies can then be used for direct Westerns. Excitation Emission • Label secondary antibodies—secondary antibodies can be labeled max. (nm) max. (nm) for traditional indirect Western blot applications l Dye-490 491 515 • Choose from 5 dyes—the non-overlapping emission spectra l Dye-550 551 565 of the fluorophores available for labeling means you can easily l Dye-650 653 672 perform multiplexing l Dye-IR7000 692 709
l Dye-IR800 783 800
Loading + Quenching Solution + Buffer All 3 channels Tubulin (green channel) + Neutralize Solution + Dye
Incubate Optional Step: Add Dye 30 Minutes Incubate 5 Minutes
Beta actin (red channel) GAPDH (blue channel) Simple labeling protocol.
Detector molecule Data
Secondary Detector Antibody molecule Data Perform multiplexing assays quick and easily. Fluorescent Western blot imaged with Azure Primary Primary c600. Secondary antibodies were labeled with Antibody Antibody the AzureSpectra labeling kit: anti-rabbit with AzureSpectra 700, anti-mouse with AzureSpectra Target Target 550, and anti-rat with AzureSpectra 800. Primary Membrane Membrane antibodies used were rat anti-tubulin, rabbit anti- Direct. Primary antibody is labeled. Indirect. Secondary antibody is beta actin, and mouse anti-GAPDH. labeled.
Part Number Name Description Size AC2185 AzureSpectra Labeling Kit – 490 Each kit includes sufficient reagents for 1 kit labeling of 2 x 50 μg of antibody. AC2186 AzureSpectra Labeling Kit – 550 1 kit • Labeling Buffer AC2187 AzureSpectra Labeling Kit – 650 • Dye solution 1 kit • Quenching solution AC2188 AzureSpectra Labeling Kit – 700 1 kit • Neutralization buffer AC2189 AzureSpectra Labeling Kit – 800 • Two G-25 columns 1 kit
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Fluorescent Detection
AzureSpectra Fluorescent Western Blotting Kits
Full kits for two-color fluorescent Western blots AzureSpectra Western Blotting Kits provide everything you need to perform two-color fluorescent Western blots. Each kit includes pre-cut membranes, wash buffer, protein-free blocking buffer, quenching sheets, and secondary antibodies to detect two proteins per experiment. Offered with Protein-Free Block or Fluorescent Block.
Two-color Western blotting. Using secondary antibody conjugate secondary antibody conjugate secondary antibodies labeled with different fluorophores, two proteins can Dye 1 Dye 2 be detected on a single blot by controlling excitation and detection channels (a). AzureSpectra conjugates incorporate primary antibody primary antibody (mouse) (rabbit) the phycobiliproteins allophycocyanin (APC) and R-phycoerythrin (RPE), extremely bright fluorescent proteins from cyanobacteria and aukaryotic algea (b,c). PVDF membrane AzureSpectra IR conjugates incorporate IR700 and IR800 fluorescent dyes with proteins transferred blocking compound near-infrared excitation and emission to the membrane wavelengths (d,e).
Part Number Name Description Size AC2140 AzureSpectra rb700/ms800 Western Kit, Kit contains materials for 10 Western Blots: 1 kit with Protein Free Block PVDF membranes, wash buffer, protein free blocking buffer, secondary antibodies AzureSpectra rb700/ms800 AC2141 AzureSpectra ms700/rb800 Western Kit, Kit contains materials for 10 Western Blots: 1 kit with Protein Free Block PVDF membranes, wash buffer, protein free blocking buffer, secondary antibodies AzureSpectra ms700/rb800 AC2104 AzureSpectra rb700/ms800 Western Kit, Kit contains materials for 10 Western Blots: 1 kit with Protein Free Block PVDF membranes, wash buffer, protein free blocking buffer, secondary antibodies rb650/ms550 AC2191 AzureSpectra rb700/ms800 Western Kit, Kit contains materials for 10 Western Blots: 1 kit with Fluorescent Block PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra rb700/ms800 AC2192 AzureSpectra ms700/rb800 Western Kit, Kit contains materials for 10 Western Blots: 1 kit with Fluorescent Block PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra ms700/rb800 AC2193 AzureSpectra rb650/ms550 Western Kit, Kit contains materials for 10 Western Blots: 1 kit with Fluorescent Block PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra rb650/ms550
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Fluorescent Detection
AzureSpectra Fluorescent Demo Kits
New to fluorescent Western blotting? Azure’s Fluorescent Demo Kits contain everything you need to perform a single color fluorescent Western using your sample and primary antibodies.
Each kit contains enough material for 1 Western blot • 9x7cm Low Fluorescence PVDF membrane • 10x Fluorescent Blot Washing Buffer, 30mL • 1x Fluorescent Blot Blocking Buffer, 30mL • Secondary Antibody, 4μL • Background Quenching Sheets, 2 sheets
Part Number Name Description Size AC2172 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Mouse 700 buffer, goat anti-mouse 700 secondary antibody AC2173 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Rabbit 700 buffer, goat anti-rabbit 700 secondary antibody AC2174 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Mouse 800 buffer, goat anti-mouse 800 secondary antibody AC2175 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Rabbit 800 buffer, goat anti-rabbit 800 secondary antibody AC2176 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Mouse 650 buffer, goat anti-mouse 650 secondary antibody AC2177 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Rabbit 650 buffer, goat anti-rabbit 650 secondary antibody AC2178 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Mouse 550 buffer, goat anti-mouse 550 secondary antibody AC2179 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Rabbit 550 buffer, goat anti-rabbit 550 secondary antibody AC2218 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Mouse 490 buffer, goat anti-mouse 490 secondary antibody AC2219 Azure Spectra Demo Kit – Kit contains 1 PVDF membrane, blocking buffer, wash 1 kit Goat Anti Rabbit 490 buffer, goat anti-rabbit 490 secondary antibody
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Ao Absorbance Microplate Reader
Assaying your 96 well plates just got easier
A microplate reader is an essential part of a productive lab, with its application flexibility, sensitivity, and high-throughput capabilities. Its top-reading design is ideal for solution-based assays such as ELISAs, DNA and protein quantification, and enzyme kinetics. • Stand-alone, top absorbance reader • Large, easy-to-use 7-inch touch screen interface • Pre-set and custom protocols • Single and dual wavelength detection modes • 8 position filter wheel: 5 standard filters (405, 450, 492, 595, and 630) plus 3 options from 340 – 750 nm • Built-in shaker with speed selection • Motorized door for easy sample loading • 2 year warranty
Specifications Ao Absorbance Microplate Reader
Plate Type 96-well Plates
Wavelength Range 340-750 nm
Filters 8 Position Filter Wheel includes 8 filters, 5 standard: 405, 450, 492, 595, and 630, and 3 selected from the filter catalogue.
Light Source Quartz-halogen Lamp
Dynamic Range 0-4.000 Abs
Resolution 0.001 Abs
Linearity (@ 405 nm) 0-2.000 Abs ≤±1%, 2-4.000Abs ≤±2%
Accuracy (@ 405 nm) 0.005 ±1% (0-3 Abs), 0.005 ±2% (3-4 Abs)
Precision (@ 405 nm) CV≤0.2% (0-3 Abs), CV≤1.0% (3-4 Abs)
Read Modes End Point, Dual Mode, Kinetic
Read Speed <6s Single Mode, 12s Dual Mode
Shaker Shaker speeds: slow, medium, fast
Data Output 3 USB Ports, Optional Printer
Dimensions (W x D x H) 440 x 295 x 225 mm
Weight 10 kg
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Ao Absorbance Microplate Reader
Part Number Name Description AC3000 Azure Ao Microplate Reader This 96-well plate, absorbance microplate reader is a stand- alone system, wavelength range 340-750nm, including 8 position filter wheel with 8 filters: 405, 450, 492, 595, and 630 standard, plus 3 from the filter catalogue, built-in shaker with speed selection, single and dual-read modes, and analysis software
Accessories
Part Number Description AC3200 USB Thermal Printer AC3201 USB Thermal Printer Paper, single roll
Filters
Part Number Description Part Number Description AC3100 Ao 340 nm Filter AC3123 Ao 562 nm Filter AC3101 Ao 380nm Filter AC3107 Ao 570 nm Filter AC3102 Ao 405 nm Filter AC3108 Ao 578 nm Filter AC3103 Ao 415 nm Filter AC3109 Ao 590 nm Filter AC3104 Ao 450 nm Filter AC3110 Ao 595 nm Filter AC3115 Ao 470 nm Filter AC3124 Ao 600 nm Filter AC3116 Ao 490 nm Filter AC3125 Ao 620 nm Filter AC3105 Ao 492 nm Filter AC3111 Ao 630 nm Filter AC3117 Ao 510 nm Filter AC3126 Ao 646 nm Filter AC3118 Ao 520 nm Filter AC3112 Ao 650 nm Filter AC3119 Ao 525 nm Filter AC3127 Ao 663 nm Filter AC3120 Ao 532 nm Filter AC3113 Ao 690 nm Filter AC3106 Ao 540 nm Filter AC3128 Ao 700 nm Filter AC3121 Ao 545 nm Filter AC3114 Ao 750 nm Filter AC3122 Ao 560 nm Filter
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New to Western blotting? Need to troubleshoot your Western blot? Want to brush up on Western blotting best practices?
Take a look at Azure Biosystems FREE “2019 Guide to Western Blotting” for the latest on fluorescent and chemiluminescent Western blots.
GET THE GUIDE azurebiosystems.com/guidebook
Complete Technical Manual • Protocols • Tips and tricks • Troubleshooting • MORE
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www.azurebiosystems.com • [email protected]
Copyright © 2019 Azure Biosystems. All rights reserved. The Azure Biosystems logo, Azure Biosystems™, cSeries™, Sapphire™ and Radiance™ are trademarks of Azure Biosystems, Inc. More information about Azure Biosystems intellectual property assets, including patents, trademarks and copyrights, is available at www.azurebiosystems.com or by contacting us by phone or email. All other 60 www.azurebiosystems.comtrademarks are property • [email protected] of their respective owners. • +1.925.307.7217