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Mechanoregulation and Pathology of YAP/TAZ Via Hippo and Non-Hippo

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Mechanoregulation and Pathology of YAP/TAZ Via Hippo and Non-Hippo Dobrokhotov et al. Clin Trans Med (2018) 7:23 https://doi.org/10.1186/s40169-018-0202-9 REVIEW Open Access Mechanoregulation and pathology of YAP/ TAZ via Hippo and non‑Hippo mechanisms Oleg Dobrokhotov1,2, Mikhail Samsonov3, Masahiro Sokabe2 and Hiroaki Hirata1,2* Abstract Yes-associated protein (YAP) and its paralog WW domain containing transcription regulator 1 (TAZ) are important regulators of multiple cellular functions such as proliferation, diferentiation, and survival. On the tissue level, YAP/ TAZ are essential for embryonic development, organ size control and regeneration, while their deregulation leads to carcinogenesis or other diseases. As an underlying principle for YAP/TAZ-mediated regulation of biological func- tions, a growing body of research reveals that YAP/TAZ play a central role in delivering information of mechanical environments surrounding cells to the nucleus transcriptional machinery. In this review, we discuss mechanical cue- dependent regulatory mechanisms for YAP/TAZ functions, as well as their clinical signifcance in cancer progression and treatment. Keywords: YAP, TAZ, Hippo pathway, Mechanotransduction, Contact inhibition of proliferation, Actomyosin, Cancer progression, Cancer therapy resistance Introduction gene expression (reviewed in [12]; and [13–16]). While Te transcriptional coactivators YAP and TAZ (Fig. 1) the Hippo pathway is the most widely known regulator have compelled researchers’ attention over the past dec- of YAP/TAZ activity, it is not the sole one. Since the frst ades as important mediators of multiple biological func- identifcation of YAP/TAZ as a mechanotransducer [17], tions. YAP/TAZ play essential roles in development, many groups have shown that YAP/TAZ are regulated homeostasis and regeneration of tissues and organs [1– in response to a number of diferent types of mechanical 6], and their dysfunctions cause several diseases [1, 4, 5, stimuli through Hippo-independent mechanisms. 7]. Both YAP and TAZ are expressed in most of human In this review, we frst discuss regulatory mechanisms tissues with some exceptions, for instance TAZ is not of YAP/TAZ focusing on their regulation by mechanical expressed in thymus and peripheral blood leukocytes, cues from cell microenvironments such as adjacent cells while YAP is not expressed in hippocampus and parathy- and the extracellular matrix (ECM). We further discuss roid gland [8–10]. the roles of YAP/TAZ in tissue homeostasis and pathol- As a canonical mechanism for YAP/TAZ regulation, ogy with particular interest in their roles in cancer pro- a phosphorylation cascade of the Hippo pathway has gression and resistance against therapeutic treatments. been identifed [11]. Tere are two major steps in Hippo Importantly, while YAP and TAZ have a lot of similari- pathway-mediated regulation of YAP/TAZ: YAP/TAZ ties in their structures, regulations and functions, they phosphorylation and nuclear translocation of YAP/TAZ. are not identical (Fig. 1; [8, 18, 19]). Terefore, in this Nuclear YAP/TAZ mainly interact with transcription review we use the term “YAP/TAZ” when there is evi- factors of the TEA domain (TEAD) family members, as dence that YAP and TAZ share the described functions well as several other transcriptional factors to regulate or regulations, whilst we specify the single protein name when evidence is available only for either YAP or TAZ. *Correspondence: [email protected]‑u.ac.jp 2 Mechanobiology Laboratory, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa‑ku, Nagoya 466‑8550, Japan Full list of author information is available at the end of the article © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat​iveco​mmons​.org/licen​ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Dobrokhotov et al. Clin Trans Med (2018) 7:23 Page 2 of 14 S127 N C YAP P-rich TEAD binding WW WW CC TAD SH3-BM PDZ-BM S89 N C TAZ TEAD binding WW CC TAD PDZ-BM Fig. 1 Domain structures of YAP and TAZ. YAP is a 65-kDa protein containing a proline-rich region (P-rich) in the N-terminal region and a PDZ-binding motif (PDZ-BM) in the C-terminal region that are separated by two WW domains, a TEAD binding domain, a Src homology domain 3 binding motif (SH3-BM), and a coiled–coil domain (CC) within the transactivation domain (TAD). There are 8 isoforms of YAP exist which difer by loss of one WW domain and alterations in TAD. TAZ, a 43-kDa paralog of YAP, has similar domain organization but lacks the proline-rich region, the SH3-BM and one WW domain. YAP S127 and TAZ S89 are the main phosphorylation targets of LATS1/2. Upon phosphorylation YAP/TAZ binds with 14-3-3 and thus are sequestered in the cytoplasm Canonical YAP/TAZ regulation via Hippo pathway cytoplasm [28, 31, 32]. Second, α-catenin does not always Te core Hippo pathway starts with MST1/2—STE20 solely localize at AJs but shows signifcant cytoplasmic family protein kinases, which phosphorylate hydrophobic attendance [27, 33]. Last, α-catenin shows a protec- motifs of LATS1/2 [20]. Phosphorylated, i.e. activated, tive efect against PP2A-mediated dephosphorylation LATS1/2 then phosphorylate serine residues in YAP/ of YAP in an in vitro dephosphorylation assay without TAZ [11, 21], leading to cytoplasmic retention of YAP/ membrane supports [27], indicating its ability to act as a TAZ [21] (Fig. 2). In addition to direct phosphorylation soluble form. As such, the cytoplasmic complex forma- of LATS1/2 by MST1/2, MTS1/2-phosphorylated SAV1 tion with α-catenin would ensure retention of YAP in the and MOB1A/B facilitate phosphorylation of LATS1/2 cytoplasm. [22–24]. While the role of MOB1A/B is not fully under- Another mechanism for inhibiting nuclear localization stood (Meng et al. proposed a model in which MOB1A/B of YAP/TAZ is phosphorylation of YAP/TAZ by casein act as scafold proteins, but it has not been experimen- K1δ/ε following the LATS-dependent phosphorylation. tally justifed yet [25]), SAV1 binds to MST1/2, targeting Such polyphosphorylation induces YAP/TAZ ubiquitina- the whole complex to the plasma membrane [26]. For tion by the SCF E3 ubiquitin ligase and subsequent deg- quite a long time, MST1/2 have been deemed to be the radation [25]. primary kinases that phosphorylate LATS1/2. However, To date, several upstream regulators of the Hippo path- it has been shown that double-depletion of MST1/2 does way have been identifed: TAO1/2/3 kinases that phos- not alter YAP phosphorylation [27, 28]. Indeed, a recent phorylate the activation loop of MST1/2 [25, 34, 35], study has shown that MAP4K family kinases act in paral- G-protein coupled receptors [36], Kibra [37], PTPN14 lel to MST1/2 to activate LATS1/2 [29]. [37], AMPK in response to energy stress [38–40], and Upon phosphorylation by LATS1/2, YAP/TAZ inter- Ras-mitogen activated protein kinase (MAPK) signalling act with 14-3-3, which sequesters YAP/TAZ from trans- [36, 41]. Moreover, while cells sufer various mechani- location into the nucleus [21]. α-Catenin, a well-known cal stimuli of diferent origins, including cell–ECM and adaptor protein localizing at cadherin-mediated cell– cell–cell contacts, ECM stifness, fuid shear, cell geom- cell junctions, stabilizes the YAP complex with 14-3-3, etry, internal actomyosin tension and so on [1, 25, 42], thereby inhibiting YAP dephosphorylation by protein these mechanical stimuli also strongly afect YAP/TAZ phosphatase 2A (PP2A) [27, 30]. It has been suggested regulation. that α-catenin acts at adherens junctions (AJ) to sta- bilize the YAP complex [27]. However, in our opinion, Impact of cell–ECM interaction on YAP/TAZ α-catenin may interact with the YAP-14-3-3 complex regulation both at and outside AJs. While this idea requires further Cells adhere to ECM through the macromolecular adhe- justifcation, there are several observations supporting sion complex called focal adhesion (FA) that links the this idea. First, exclusion of YAP from the nucleus usu- actin cytoskeleton to ECM [43–45]. While cells sense ally does not lead to its plasma membrane recruitment, stifness of ECM and change their spreading and migra- rather it more or less evenly distributes throughout the tion in response to ECM stifness [17, 31, 46–52], YAP/ Dobrokhotov et al. Clin Trans Med (2018) 7:23 Page 3 of 14 Stiff and Fibronectin-rich ECM Fig. 2 Overview of signaling cascades for YAP/TAZ regulation. Hippo pathway- (blue), FA- (pink) and AJ-mediated (yellow) regulations of YAP/TAZ are shown. See the main text for details TAZ activity is regulated by ECM stifness and cell and Src–Rac1–PAK axes form a single cascade or act in spreading. Spread cells and cells grown on stif ECM parallel. show elevated YAP/TAZ activity which correlates with On the other hand, Dupont et al. reported that their nuclear localization [17, 31, 48, 53]. mechanical cue-induced regulation of YAP in human Mechanical environments characterized by cell mor- mammary epithelial cells and human mesenchymal stem phology and the cell–ECM contact area regulate YAP cells is independent of the Hippo pathway [17], which nuclear localization via afecting LATS1/2-dependent was later confrmed by other groups [31, 48, 58]. How- YAP phosphorylation [53]. As an underlying molecu- ever, for a long time, the mechanism that provides con- lar mechanism, involvement of integrin signalling has sistent explanation for the mechanical regulation of YAP/ been revealed (Figs. 2 and 3). Stif or fbronectin-rich TAZ has been unsolved.
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