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Dysfunctional Mechanotransduction Through the YAP/TAZ/Hippo Pathway As a Feature of Chronic Disease
cells Review Dysfunctional Mechanotransduction through the YAP/TAZ/Hippo Pathway as a Feature of Chronic Disease 1, 2, 2,3, 4 Mathias Cobbaut y, Simge Karagil y, Lucrezia Bruno y, Maria Del Carmen Diaz de la Loza , Francesca E Mackenzie 3, Michael Stolinski 2 and Ahmed Elbediwy 2,* 1 Protein Phosphorylation Lab, Francis Crick Institute, London NW1 1AT, UK; [email protected] 2 Department of Biomolecular Sciences, Kingston University, Kingston-upon-Thames KT1 2EE, UK; [email protected] (S.K.); [email protected] (L.B.); [email protected] (M.S.) 3 Department of Chemical and Pharmaceutical Sciences, Kingston University, Kingston-upon-Thames KT1 2EE, UK; [email protected] 4 Epithelial Biology Lab, Francis Crick Institute, London NW1 1AT, UK; [email protected] * Correspondence: [email protected] These authors contribute equally to this work. y Received: 30 November 2019; Accepted: 4 January 2020; Published: 8 January 2020 Abstract: In order to ascertain their external environment, cells and tissues have the capability to sense and process a variety of stresses, including stretching and compression forces. These mechanical forces, as experienced by cells and tissues, are then converted into biochemical signals within the cell, leading to a number of cellular mechanisms being activated, including proliferation, differentiation and migration. If the conversion of mechanical cues into biochemical signals is perturbed in any way, then this can be potentially implicated in chronic disease development and processes such as neurological disorders, cancer and obesity. This review will focus on how the interplay between mechanotransduction, cellular structure, metabolism and signalling cascades led by the Hippo-YAP/TAZ axis can lead to a number of chronic diseases and suggest how we can target various pathways in order to design therapeutic targets for these debilitating diseases and conditions. -
Ryanodine Receptors Are Part of the Myospryn Complex in Cardiac Muscle Received: 10 March 2017 Matthew A
www.nature.com/scientificreports OPEN Ryanodine receptors are part of the myospryn complex in cardiac muscle Received: 10 March 2017 Matthew A. Benson1, Caroline L. Tinsley2, Adrian J. Waite 2, Francesca A. Carlisle2, Steve M. Accepted: 12 June 2017 M. Sweet3, Elisabeth Ehler4, Christopher H. George5, F. Anthony Lai5,6, Enca Martin-Rendon7 Published online: 24 July 2017 & Derek J. Blake 2 The Cardiomyopathy–associated gene 5 (Cmya5) encodes myospryn, a large tripartite motif (TRIM)- related protein found predominantly in cardiac and skeletal muscle. Cmya5 is an expression biomarker for a number of diseases afecting striated muscle and may also be a schizophrenia risk gene. To further understand the function of myospryn in striated muscle, we searched for additional myospryn paralogs. Here we identify a novel muscle-expressed TRIM-related protein minispryn, encoded by Fsd2, that has extensive sequence similarity with the C-terminus of myospryn. Cmya5 and Fsd2 appear to have originated by a chromosomal duplication and are found within evolutionarily-conserved gene clusters on diferent chromosomes. Using immunoafnity purifcation and mass spectrometry we show that minispryn co-purifes with myospryn and the major cardiac ryanodine receptor (RyR2) from heart. Accordingly, myospryn, minispryn and RyR2 co-localise at the junctional sarcoplasmic reticulum of isolated cardiomyocytes. Myospryn redistributes RyR2 into clusters when co-expressed in heterologous cells whereas minispryn lacks this activity. Together these data suggest a novel role for the myospryn complex in the assembly of ryanodine receptor clusters in striated muscle. Te unique cytoskeletal organisation of striated muscle is dependent upon the formation of specialised interac- tions between proteins that have both structural and signalling functions1. -
The Hippo Pathway Component Wwc2 Is a Key Regulator of Embryonic Development and Angiogenesis in Mice Anke Hermann1,Guangmingwu2, Pavel I
Hermann et al. Cell Death and Disease (2021) 12:117 https://doi.org/10.1038/s41419-021-03409-0 Cell Death & Disease ARTICLE Open Access The Hippo pathway component Wwc2 is a key regulator of embryonic development and angiogenesis in mice Anke Hermann1,GuangmingWu2, Pavel I. Nedvetsky1,ViktoriaC.Brücher3, Charlotte Egbring3, Jakob Bonse1, Verena Höffken1, Dirk Oliver Wennmann1, Matthias Marks4,MichaelP.Krahn 1,HansSchöler5,PeterHeiduschka3, Hermann Pavenstädt1 and Joachim Kremerskothen1 Abstract The WW-and-C2-domain-containing (WWC) protein family is involved in the regulation of cell differentiation, cell proliferation, and organ growth control. As upstream components of the Hippo signaling pathway, WWC proteins activate the Large tumor suppressor (LATS) kinase that in turn phosphorylates Yes-associated protein (YAP) and its paralog Transcriptional coactivator-with-PDZ-binding motif (TAZ) preventing their nuclear import and transcriptional activity. Inhibition of WWC expression leads to downregulation of the Hippo pathway, increased expression of YAP/ TAZ target genes and enhanced organ growth. In mice, a ubiquitous Wwc1 knockout (KO) induces a mild neurological phenotype with no impact on embryogenesis or organ growth. In contrast, we could show here that ubiquitous deletion of Wwc2 in mice leads to early embryonic lethality. Wwc2 KO embryos display growth retardation, a disturbed placenta development, impaired vascularization, and finally embryonic death. A whole-transcriptome analysis of embryos lacking Wwc2 revealed a massive deregulation of gene expression with impact on cell fate determination, 1234567890():,; 1234567890():,; 1234567890():,; 1234567890():,; cell metabolism, and angiogenesis. Consequently, a perinatal, endothelial-specific Wwc2 KO in mice led to disturbed vessel formation and vascular hypersprouting in the retina. -
Hippo Signaling Regulates Drosophila Intestine Stem Cell Proliferation Through Multiple Pathways
Hippo signaling regulates Drosophila intestine stem cell proliferation through multiple pathways Fangfang Rena, Bing Wanga, Tao Yuea, Eun-Young Yunb,1, Y. Tony Ipb, and Jin Jianga,c,2 aDepartment of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390; bProgram in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605; and cDepartment of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390 Edited* by Michael Levine, University of California, Berkeley, CA, and approved October 6, 2010 (received for review August 27, 2010) Intestinal stem cells (ISCs) in the Drosophila adult midgut are essen- investigated. In this study, we demonstrate that the Hpo pathway tial for maintaining tissue homeostasis and replenishing lost cells in restricts ISC proliferation in the adult midgut. We uncover response to tissue damage. Here we demonstrate that the Hippo a non–cell-autonomous mechanism by which the Hpo pathway (Hpo) signaling pathway, an evolutionarily conserved pathway im- negatively regulates stem cell proliferation by restricting the plicated in organ size control and tumorigenesis, plays an essential production of cytokines and mitogens that activate the JAK- role in regulating ISC proliferation. Loss of Hpo signaling in either STAT and EGFR pathways. In addition, we demonstrate that midgut precursor cells or epithelial cells stimulates ISC proliferation. Yki is required in the precursor cells for dextran sulfate sodium We provide evidence that loss of Hpo signaling in epithelial cells (DSS)-induced ISC proliferation. increases the production of cytokines of the Upd family and multiple EGFR ligands that activate JAK-STAT and EGFR signaling pathways Results in ISCs to stimulate their proliferation, thus revealing a unique non– Loss of Hpo Signaling in Precursor Cells Stimulates ISC Proliferation. -
Dystrophin Complex Functions As a Scaffold for Signalling Proteins☆
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Biochimica et Biophysica Acta 1838 (2014) 635–642 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journal homepage: www.elsevier.com/locate/bbamem Review Dystrophin complex functions as a scaffold for signalling proteins☆ Bruno Constantin IPBC, CNRS/Université de Poitiers, FRE 3511, 1 rue Georges Bonnet, PBS, 86022 Poitiers, France article info abstract Article history: Dystrophin is a 427 kDa sub-membrane cytoskeletal protein, associated with the inner surface membrane and Received 27 May 2013 incorporated in a large macromolecular complex of proteins, the dystrophin-associated protein complex Received in revised form 22 August 2013 (DAPC). In addition to dystrophin the DAPC is composed of dystroglycans, sarcoglycans, sarcospan, dystrobrevins Accepted 28 August 2013 and syntrophin. This complex is thought to play a structural role in ensuring membrane stability and force trans- Available online 7 September 2013 duction during muscle contraction. The multiple binding sites and domains present in the DAPC confer the scaf- fold of various signalling and channel proteins, which may implicate the DAPC in regulation of signalling Keywords: Dystrophin-associated protein complex (DAPC) processes. The DAPC is thought for instance to anchor a variety of signalling molecules near their sites of action. syntrophin The dystroglycan complex may participate in the transduction of extracellular-mediated signals to the muscle Sodium channel cytoskeleton, and β-dystroglycan was shown to be involved in MAPK and Rac1 small GTPase signalling. More TRPC channel generally, dystroglycan is view as a cell surface receptor for extracellular matrix proteins. -
Hippo Signaling Pathway As a New Potential Target in Non-Melanoma Skin Cancers: a Narrative Review
life Review Hippo Signaling Pathway as a New Potential Target in Non-Melanoma Skin Cancers: A Narrative Review Igor Aleksander Bednarski 1,*, Magdalena Ci ˛azy˙ ´nska 2 , Karolina Wódz 3, Izabela Drózd˙ z˙ 4 , Małgorzata Skibi ´nska 1, Joanna Narbutt 1 and Aleksandra Lesiak 1 1 Department of Dermatology, Pediatric Dermatology and Dermatological Oncology, Medical University of Lodz, 91-347 Lodz, Poland; [email protected] (M.S.); [email protected] (J.N.); [email protected] (A.L.) 2 Department of Proliferative Diseases, Nicolaus Copernicus Multidisciplinary Centre for Oncology and Traumatology, 93-513 Lodz, Poland; [email protected] 3 Laboratory of Molecular Biology, VET-LAB Brudzew, 62-720 Brudzew, Poland; [email protected] 4 Department of Clinical Genetics, Medical University of Lodz, Pomorska 251, 92-213 Lodz, Poland; [email protected] * Correspondence: [email protected] Abstract: Non-melanoma skin cancers (NMSCs), including basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (cSCC), are the most frequently diagnosed cancers in humans, however, their exact pathogenesis is not fully understood. In recent years, it has been hypothesized that the recently discovered Hippo pathway could play a detrimental role in cutaneous carcinogenesis, but no direct connections have been made. The Hippo pathway and its effector, YAP, are responsible for tissue growth by accelerating cell proliferation, however, YAP upregulation and overexpression have also been reported in numerous types of tumors. There is also evidence that disrupted YAP/Hippo Citation: Bednarski, I.A.; Ci ˛azy´nska,˙ signaling is responsible for cancer growth, invasion, and metastasis. -
Type I and II Interferon Are Associated with High Expression of the Hippo Pathway Family Members
Cel al & lul ic ar in I l m C m f o u n l a o l n o Journal of Clinical & Cellular r g u y o J ISSN: 2155-9899 Immunology Research Article Type I and II Interferon are Associated with High Expression of the Hippo Pathway Family Members Bianca Sciescia1*, Raquel Tognon2, Natalia de Souza Nunes1, Tathiane Maistro Malta1, Fabiani Gai Frantz1, Fabiola Attie de Castro1, Maira da Costa Cacemiro1 1Department of Clinical, Toxicological and Bromatological Analysis, University of Sao Paulo - USP, Ribeirao Preto - SP, Brazil; 2Department of Pharmacy, Federal University of Juiz de Fora, Campus Governador Valadares, Governador Valadares - MG, Brazil ABSTRACT The Hippo pathway plays a regulatory role on inflammation and cell death and proliferation. Here we described a relationship between Hippo pathway components and inflammation in healthy subjects. The plasma levels of cytokines and chemokines were used to define their inflammatory profile and classify them as normal, high and low producers of cytokines. Leukocytes from healthy subjects with inflammatory profile expressed the highest levels of MSTS1/MST2, SAV1, LATS1/LATS2, MOB1A/MOB1B and YAP genes. The group that overexpressed Hippo pathway-related genes secreted more IFN-ϒ and IFN-α2. Keywords: Hippo pathway; Inflammatory process; Healthy subjects; Cytokines; Chemokines ABBREVATIONS: LATS1/LATS2 kinases (large tumor suppressor kinase 1/2) and MOB kinase activator IL: Interleukin; IFN: Interferon; LATS1/LATS2: Large tumor their adapter proteins MOB1A/MOB1B ( 1A/1B yes-associated suppressor kinase 1/2; MCP-1: Monocyte chemoattractant ), and the transcription coactivators YAP ( protein tafazzin protein protein-1; MIP: Macrophage inflammatory protein; MOB1A/ ) and TAZ ( ). -
Hippo Signaling Antibody Sampler
Hippo Signaling Antibody Sampler Kit Store at -20°C 3 n 1 Kit Orders n 877-616-CELL (2355) (9 x 20 µl) [email protected] Support n 877-678-TECH (8324) [email protected] Web n www.cellsignal.com rev. 06/16 #8579 For Research Use Only. Not For Use In Diagnostic Procedures. Products Included Product # Quantity Mol. Wt. Isotype Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb 13619 20 µl 75 kDa Rabbit IgG sodium azide. Store at –20°C. Do not aliquot the antibodies. LATS1 (C66B5) Rabbit mAb 3477 20 µl 140 kDa Rabbit IgG Recommended Antibody Dilutions: Western blotting 1:1000 Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb 8699 20 µl 24 kDa Rabbit IgG Please visit www.cellsignal.com for validation data MOB1 (E1N9D) Rabbit mAb 13730 20 µl 25 kDa Rabbit IgG and a complete listing of recommended companion Mst1 Antibody 3682 20 µl 59 kDa Rabbit IgG products. Mst2 Antibody 3952 20 µl 60 kDa Rabbit IgG SAV1 (D6M6X) Rabbit mAb 13301 20 µl 45 kDa Rabbit IgG Phospho-YAP (Ser127) (D9W2I) Rabbit mAb 13008 20 µl 65-75 kDa Rabbit IgG YAP/TAZ (D24E4) Rabbit mAb 8418 20 µl 50,70 kDa Rabbit IgG Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat See www.cellsignal.com for individual component applications, species cross-reactivity, dilutions and additional application protocols. Description: The Hippo Signaling Antibody Sampler Kit MOB1 (Thr35) (D2F10) Rabbit mAb recognizes endogenous Background References: provides an economical means of detecting target proteins levels of MOB1 protein only when phosphorylated at Thr35. -
Modulation of Hippo Pathway by Alternative Splicing Diwas Srivastava
Modulation of hippo pathway by alternative splicing Diwas Srivastava To cite this version: Diwas Srivastava. Modulation of hippo pathway by alternative splicing. Agricultural sciences. Uni- versité Montpellier, 2019. English. NNT : 2019MONTT015. tel-02387314 HAL Id: tel-02387314 https://tel.archives-ouvertes.fr/tel-02387314 Submitted on 29 Nov 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE POUR OBTENIR LE GRADE DE DOCTEUR DE L’UNIVERSITÉ DE M ONTPELLIER En BIOLOGIE - SANTE École doctorale- Biologiques pour la Santé (CBS2) Unité de recherche- Institut de Génétique Moléculaire de Montpellier Modulation of Hippo Pathway by Alternative Splicing Présentée par Diwas SRIVASTAVA Le 25 Juin 2019 Sous la direction de Dr. François JUGE et Dr. Jamal TAZI Devant le jury composé de Frédérique Peronnet , DR, HDR Institut de Biologie- Paris Seine RAPPORTRICE Julien Colombani, CR, HDR Université de Copenhague- Danemark RAPPORTEUR Florence Besse, DR, HDR Institute of Biology Valrose EXAMINATRICE Anne-Marie Martinez, Pr, HDR Université de Montpellier PRESIDENTE, EXAMINATRICE Francois Juge, CR,HDR Institut de Génétique Moléculaire de Montpellier DIRECTEUR DE THESE Jamal Tazi, Pr, HDR Université de Montpellier CO-DIRECTEUR DE THESE Acknowledgements First, I would like to express my deepest gratitude to my supervisor, Dr. -
Hippo Signaling Interactions with Wnt/Β-Catenin and Notch Signaling Repress Liver Tumorigenesis
The Journal of Clinical Investigation RESEARCH ARTICLE Hippo signaling interactions with Wnt/β-catenin and Notch signaling repress liver tumorigenesis Wantae Kim,1,2 Sanjoy Kumar Khan,1,2 Jelena Gvozdenovic-Jeremic,1 Youngeun Kim,3 Jason Dahlman,1 Hanjun Kim,1,2 Ogyi Park,4 Tohru Ishitani,5 Eek-hoon Jho,3 Bin Gao,4 and Yingzi Yang1,2 1Genetic Disease Research Branch, National Human Genome Research Institute (NHGRI), NIH, Bethesda, Maryland, USA. 2Department of Developmental Biology, Harvard School of Dental Medicine (HSDM), Boston, Massachusetts, USA. 3Department of Life Sciences, University of Seoul, Seoul, South Korea. 4Section on Liver Biology, National Institute on Alcohol Abuse and Alcoholism (NIAAA), NIH, Bethesda, Maryland, USA. 5Division of Cell Regulation Systems, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. Malignant tumors develop through multiple steps of initiation and progression, and tumor initiation is of singular importance in tumor prevention, diagnosis, and treatment. However, the molecular mechanism whereby a signaling network of interacting pathways restrains proliferation in normal cells and prevents tumor initiation is still poorly understood. Here, we have reported that the Hippo, Wnt/β-catenin, and Notch pathways form an interacting network to maintain liver size and suppress hepatocellular carcinoma (HCC). Ablation of the mammalian Hippo kinases Mst1 and Mst2 in liver led to rapid HCC formation and activated Yes-associated protein/WW domain containing transcription regulator 1 (YAP/TAZ), STAT3, Wnt/β-catenin, and Notch signaling. Previous work has shown that abnormal activation of these downstream pathways can lead to HCC. Rigorous genetic experiments revealed that Notch signaling forms a positive feedback loop with the Hippo signaling effector YAP/TAZ to promote severe hepatomegaly and rapid HCC initiation and progression. -
Genomics of Inherited Bone Marrow Failure and Myelodysplasia Michael
Genomics of inherited bone marrow failure and myelodysplasia Michael Yu Zhang A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy University of Washington 2015 Reading Committee: Mary-Claire King, Chair Akiko Shimamura Marshall Horwitz Program Authorized to Offer Degree: Molecular and Cellular Biology 1 ©Copyright 2015 Michael Yu Zhang 2 University of Washington ABSTRACT Genomics of inherited bone marrow failure and myelodysplasia Michael Yu Zhang Chair of the Supervisory Committee: Professor Mary-Claire King Department of Medicine (Medical Genetics) and Genome Sciences Bone marrow failure and myelodysplastic syndromes (BMF/MDS) are disorders of impaired blood cell production with increased leukemia risk. BMF/MDS may be acquired or inherited, a distinction critical for treatment selection. Currently, diagnosis of these inherited syndromes is based on clinical history, family history, and laboratory studies, which directs the ordering of genetic tests on a gene-by-gene basis. However, despite extensive clinical workup and serial genetic testing, many cases remain unexplained. We sought to define the genetic etiology and pathophysiology of unclassified bone marrow failure and myelodysplastic syndromes. First, to determine the extent to which patients remained undiagnosed due to atypical or cryptic presentations of known inherited BMF/MDS, we developed a massively-parallel, next- generation DNA sequencing assay to simultaneously screen for mutations in 85 BMF/MDS genes. Querying 71 pediatric and adult patients with unclassified BMF/MDS using this assay revealed 8 (11%) patients with constitutional, pathogenic mutations in GATA2 , RUNX1 , DKC1 , or LIG4 . All eight patients lacked classic features or laboratory findings for their syndromes. -
©Ferrata Storti Foundation
Original Articles T-cell/histiocyte-rich large B-cell lymphoma shows transcriptional features suggestive of a tolerogenic host immune response Peter Van Loo,1,2,3 Thomas Tousseyn,4 Vera Vanhentenrijk,4 Daan Dierickx,5 Agnieszka Malecka,6 Isabelle Vanden Bempt,4 Gregor Verhoef,5 Jan Delabie,6 Peter Marynen,1,2 Patrick Matthys,7 and Chris De Wolf-Peeters4 1Department of Molecular and Developmental Genetics, VIB, Leuven, Belgium; 2Department of Human Genetics, K.U.Leuven, Leuven, Belgium; 3Bioinformatics Group, Department of Electrical Engineering, K.U.Leuven, Leuven, Belgium; 4Department of Pathology, University Hospitals K.U.Leuven, Leuven, Belgium; 5Department of Hematology, University Hospitals K.U.Leuven, Leuven, Belgium; 6Department of Pathology, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway, and 7Department of Microbiology and Immunology, Rega Institute for Medical Research, K.U.Leuven, Leuven, Belgium Citation: Van Loo P, Tousseyn T, Vanhentenrijk V, Dierickx D, Malecka A, Vanden Bempt I, Verhoef G, Delabie J, Marynen P, Matthys P, and De Wolf-Peeters C. T-cell/histiocyte-rich large B-cell lymphoma shows transcriptional features suggestive of a tolero- genic host immune response. Haematologica. 2010;95:440-448. doi:10.3324/haematol.2009.009647 The Online Supplementary Tables S1-5 are in separate PDF files Supplementary Design and Methods One microgram of total RNA was reverse transcribed using random primers and SuperScript II (Invitrogen, Merelbeke, Validation of microarray results by real-time quantitative Belgium), as recommended by the manufacturer. Relative reverse transcriptase polymerase chain reaction quantification was subsequently performed using the compar- Ten genes measured by microarray gene expression profil- ative CT method (see User Bulletin #2: Relative Quantitation ing were validated by real-time quantitative reverse transcrip- of Gene Expression, Applied Biosystems).