BASIDIOMYCOTINA and ITS CLASSIFICATION Dr
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Bulk Isolation of Basidiospores from Wild Mushrooms by Electrostatic Attraction with Low Risk of Microbial Contaminations Kiran Lakkireddy1,2 and Ursula Kües1,2*
Lakkireddy and Kües AMB Expr (2017) 7:28 DOI 10.1186/s13568-017-0326-0 ORIGINAL ARTICLE Open Access Bulk isolation of basidiospores from wild mushrooms by electrostatic attraction with low risk of microbial contaminations Kiran Lakkireddy1,2 and Ursula Kües1,2* Abstract The basidiospores of most Agaricomycetes are ballistospores. They are propelled off from their basidia at maturity when Buller’s drop develops at high humidity at the hilar spore appendix and fuses with a liquid film formed on the adaxial side of the spore. Spores are catapulted into the free air space between hymenia and fall then out of the mushroom’s cap by gravity. Here we show for 66 different species that ballistospores from mushrooms can be attracted against gravity to electrostatic charged plastic surfaces. Charges on basidiospores can influence this effect. We used this feature to selectively collect basidiospores in sterile plastic Petri-dish lids from mushrooms which were positioned upside-down onto wet paper tissues for spore release into the air. Bulks of 104 to >107 spores were obtained overnight in the plastic lids above the reversed fruiting bodies, between 104 and 106 spores already after 2–4 h incubation. In plating tests on agar medium, we rarely observed in the harvested spore solutions contamina- tions by other fungi (mostly none to up to in 10% of samples in different test series) and infrequently by bacteria (in between 0 and 22% of samples of test series) which could mostly be suppressed by bactericides. We thus show that it is possible to obtain clean basidiospore samples from wild mushrooms. -
BIOL 1030 – TOPIC 3 LECTURE NOTES Topic 3: Fungi (Kingdom Fungi – Ch
BIOL 1030 – TOPIC 3 LECTURE NOTES Topic 3: Fungi (Kingdom Fungi – Ch. 31) KINGDOM FUNGI A. General characteristics • Fungi are diverse and widespread. • Ten thousand species of fungi have been described, but it is estimated that there are actually up to 1.5 million species of fungi. • Fungi play an important role in ecosystems, decomposing dead organisms, fallen leaves, feces, and other organic materials. °This decomposition recycles vital chemical elements back to the environment in forms other organisms can assimilate. • Most plants depend on mutualistic fungi to help their roots absorb minerals and water from the soil. • Humans have cultivated fungi for centuries for food, to produce antibiotics and other drugs, to make bread rise, and to ferment beer and wine • Fungi play ecological diverse roles - they are decomposers (saprobes), parasites, and mutualistic symbionts. °Saprobic fungi absorb nutrients from nonliving organisms. °Parasitic fungi absorb nutrients from the cells of living hosts. .Some parasitic fungi, including some that infect humans and plants, are pathogenic. .Fungi cause 80% of plant diseases. °Mutualistic fungi also absorb nutrients from a host organism, but they reciprocate with functions that benefit their partner in some way. • Fungi are a monophyletic group, and all fungi share certain key characteristics. B. Morphology of Fungi 1. heterotrophs - digest food with secreted enzymes “exoenzymes” (external digestion) 2. have cell walls made of chitin 3. most are multicellular, with slender filamentous units called hyphae (Label the diagram below – Use Textbook figure 31.3) 1 of 11 BIOL 1030 – TOPIC 3 LECTURE NOTES Septate hyphae Coenocytic hyphae hyphae may be divided into cells by crosswalls called septa; typically, cytoplasm flows through septa • hyphae can form specialized structures for things such as feeding, and even for food capture 4. -
Diversity of Fungi 10 Mar KB – Plant Function Chapters 38, 40, and 39 (Pp
Upcoming Syllabus (middle third) 24 Feb KB – Fungi Chapter 31 26 Feb KB – Prokaryotes, Protists, Photoautotrophy, Endosymbioses Chapters 28, 29 3 Mar KB – Plant Diversity Chapter 30 5 Mar KB – Plant Form and Function Chapters 36, 37 Diversity of Fungi 10 Mar KB – Plant Function Chapters 38, 40, and 39 (pp. 857-866, 873-882, 887-888) (Freeman Ch31) 12 Mar WS - Population Growth and Regulation Chapter 52 17&19 Mar Spring Recess 24 Mar KB – Plant Community Ecology, Disturbance, Succession Chapters 30, 53 26 Mar KB – Galapagos Case Study Wikelski 2000 and www.darwinfoundation.org/en/galapagos/marine www.darwinfoundation.org/en/galapagos/land 24 February 2009 31 Mar Part 2. Discussion and Review. ECOL 182R UofA 1 2 Thanks to Joanna Masel 02 Apr EXAM 2 K. E. Bonine VIDEOS Kevin Bonine Tree of Life 182 Office Hours 3.8 bya 10-noon Tuesdays BSE 113 2+ bya -also M 1-2 and W 11-noon- -206 and 437 students have priority- Orange 3 4 Opisthokonts (Fungi and Animals are closely related) 5 6 1 Chitin How fungi live (tough but flexible nitrogen-containing polysaccharide) • All use absorptive nutrition, secreting •Production of chitin is a shared derived digestive enzymes and absorbing the trait for breakdown products –fungi •Most are saprobes (feed on dead matter) – choanoflagellates – Earth’s main decomposers (with bacteria) –animals – principal decomposers of cellulose & lignin • Evidence that fungi are closer to – nutrient (re)cyclers animals than plants •Some are parasites •A few are mutualists 7 8 Cell structure of multicellular fungi Incomplete -
Fungus Populations M Marine Waters and Coastal Sands of the Hawaiian, Line, and Phoenix Islands' CAROL WRIGHT STEE LE2
Fungus Populations m Marine Waters and Coastal Sands of the Hawaiian, Line, and Phoenix Islands' CAROL WRIGHT STEE LE2 ABSTRACT: Saprophytic and facultative parasitic fungi present in the coastal waters and adjacent pelagic areas of the Hawaiian Islands, and in coastal sands of the H awaiian, Line, and Phoenix islands, were isolated by plating methods. Isolates from all areas sampled indicate that abundant and varied fungus popu lations do exist in these environments. The number of fungi obtained from the inshore neritic zone was seven times that obtained from the oceanic zone. The fungus Anreobasidium pltllttla11S ( De Bary) Arnaud was isolated repeatedly from oceanic waters. A comparison is made between the genera and the average number of isolates per liter of water known from the Atlantic Ocean with those found in this study of the Pacific Ocean. The number of fungi isolated from sand samples of the different islands ranged from 2 to 1,600 per gram. Species diversity was evident throughout the samples. The leeward Hawaiian islands had a higher aver age number of isolates per gram than any other island group. In conclusion the problems of defining a marine fungus are discussed. OCEA NIC AREAS in different parts of the world also are very limited. These include a few rec have been shown to be habitats for marine ords of higher fungi collected in the Marshall fungi (Johnson and Sparrow, 1961) . Investi Islands (Rogers, 1947) , the Society Islands gators, however, have usually concentrated on (Olive, 1957, 1958) , and Raroia in the Tua particular groups of fungi by use of selective motu Archipelago (Cooke, 1961); Phyco isolation methods ( Barghoorn and Linder, mycetes recovered by plating soils of the atolls 1944; Moore and Meyers, 1959; Jones, 1962) . -
Yeasts in Pucciniomycotina
Mycol Progress DOI 10.1007/s11557-017-1327-8 REVIEW Yeasts in Pucciniomycotina Franz Oberwinkler1 Received: 12 May 2017 /Revised: 12 July 2017 /Accepted: 14 July 2017 # German Mycological Society and Springer-Verlag GmbH Germany 2017 Abstract Recent results in taxonomic, phylogenetic and eco- to conjugation, and eventually fructificaction (Brefeld 1881, logical studies of basidiomycetous yeast research are remark- 1888, 1895a, b, 1912), including mating experiments (Bauch able. Here, Pucciniomycotina with yeast stages are reviewed. 1925; Kniep 1928). After an interval, yeast culture collections The phylogenetic origin of single-cell basidiomycetes still re- were established in various institutions and countries, and mains unsolved. But the massive occurrence of yeasts in basal yeast manuals (Lodder and Kreger-van Rij 1952;Lodder basidiomycetous taxa indicates their early evolutionary pres- 1970;Kreger-vanRij1984; Kurtzman and Fell 1998; ence. Yeasts in Cryptomycocolacomycetes, Mixiomycetes, Kurtzman et al. 2011) were published, leading not only to Agaricostilbomycetes, Cystobasidiomycetes, Septobasidiales, the impression, but also to the practical consequence, that, Heterogastridiomycetes, and Microbotryomycetes will be most often, researchers studying yeasts were different from discussed. The apparent loss of yeast stages in mycologists and vice versa. Though it was well-known that Tritirachiomycetes, Atractiellomycetes, Helicobasidiales, a yeast, derived from a fungus, represents the same species, Platygloeales, Pucciniales, Pachnocybales, and most scientists kept to the historical tradition, and, even at the Classiculomycetes will be mentioned briefly for comparative same time, the superfluous ana- and teleomorph terminology purposes with dimorphic sister taxa. Since most phylogenetic was introduced. papers suffer considerably from the lack of adequate illustra- In contrast, biologically meaningful academic teaching re- tions, plates for representative species of orders have been ar- quired rethinking of the facts and terminology, which very ranged. -
Basidiomycotina
BASIDIOMYCOTINA General Characters • Most advanced group of all fungal classes • Comprises of about 500 genera and 15,000 species • Includes both saprophytic and parasitic species (Mushrooms, Puffballs, Toad stools, Rusts, Smuts etc.) • Parasitic genera spread on stem, leaves, wood or inflorescence (Ustilago, Puccinia, Polyporus, Ganoderma etc.) • Saprophytic species live in decaying wood, logs, dung, dead leaves and humus rich soil (Agaricus, Lycoperdon, Pleurotus, Cyathus, Geastrum etc.) • Characteristics spores are basidiospores, produced on basidia Mycelium • Mycelium is branched, well developed and perennial • Spreading in a fan shaped manner – forming fairy rings in mushrooms • Mycelium spread on the substratum and absorb food • In few genera mycelium form rhizomorphs • Hyphae are septate, septal pore is surrounded by a swollen rim or crescent shaped cap – parenthesome – such septa are called dolipore septum • Not seen in rusts and smuts • Cell wall is made up of chitin • Mycelium occur in three stages • Primary mycelium: Monokaryotic, short lived, formed by germination of basidiospores, represent haplophase, does not bear any sex organs • Secondary mycelium: Dikaryotic, perennial, formed by the fusion (dikaryotisation) of two dissimilar primary mycelium. Represent dikaryotic phase, produce fruiting bodies and show clamp connections • Tertiary mycelium: In higher members, secondary mycelium get organised into specialized tissues forming fruiting bodies. Dikaryotic in nature Clamp Connections • Dikaryotic secondary mycelium grow by -
Studies in the Stypella Vermiformis Group (Auriculariales, Basidiomycota)
Studies in the Stypella vermiformis group (Auriculariales, Basidiomycota) Viacheslav Spirin, Vera Malysheva, Danny Haelewaters & Karl-Henrik Larsson Antonie van Leeuwenhoek Journal of Microbiology ISSN 0003-6072 Volume 112 Number 5 Antonie van Leeuwenhoek (2019) 112:753-764 DOI 10.1007/s10482-018-01209-9 1 23 Your article is published under the Creative Commons Attribution license which allows users to read, copy, distribute and make derivative works, as long as the author of the original work is cited. You may self- archive this article on your own website, an institutional repository or funder’s repository and make it publicly available immediately. 1 23 Antonie van Leeuwenhoek (2019) 112:753–764 https://doi.org/10.1007/s10482-018-01209-9 (0123456789().,-volV)( 0123456789().,-volV) ORIGINAL PAPER Studies in the Stypella vermiformis group (Auriculariales, Basidiomycota) Viacheslav Spirin . Vera Malysheva . Danny Haelewaters . Karl-Henrik Larsson Received: 27 June 2018 / Accepted: 30 November 2018 / Published online: 8 December 2018 Ó The Author(s) 2018 Abstract Stypella vermiformis is a heterobasid- three species from two newly described genera. S. iomycete producing minute gelatinous basidiocarps vermiformis s.str. is distributed in temperate Europe on rotten wood of conifers in the Northern Hemi- and has small-sized basidia and basidiospores, and it is sphere. In the current literature, Stypella papillata, the placed in a new genus, Mycostilla. Another genus, genus type of Stypella (described from Brazil), is Stypellopsis, is created for two other species, the North treated as a taxonomic synonym of S. vermiformis.In American Stypellopsis farlowii, comb. nov., and the the present paper, we revise the type material of S. -
Mycology Guidebook. INSTITUTICN Mycological Society of America, San Francisco, Calif
DOCUMENT BEMIRE ED 174 459 SE 028 530 AUTHOR Stevens, Russell B., Ed. TITLE Mycology Guidebook. INSTITUTICN Mycological Society of America, San Francisco, Calif. SPCNS AGENCY National Science Foundation, Washington, D.C. PUB DATE 74 GRANT NSF-GE-2547 NOTE 719p. EDPS PRICE MF04/PC29 Plus Postage. DESCRIPSCRS *Biological Sciences; College Science; *Culturing Techniques; Ecology; *Higher Education; *Laboratory Procedures; *Resource Guides; Science Education; Science Laboratories; Sciences; *Taxonomy IDENTIFIERS *National Science Foundation ABSTT.RACT This guidebook provides information related to developing laboratories for an introductory college-level course in mycology. This information will enable mycology instructors to include information on less-familiar organisms, to diversify their courses by introducing aspects of fungi other than the more strictly taxcncnic and morphologic, and to receive guidance on fungi as experimental organisms. The text is organized into four parts: (1) general information; (2) taxonomic groups;(3) ecological groups; and (4) fungi as biological tools. Data and suggestions are given for using fungi in discussing genetics, ecology, physiology, and other areas of biology. A list of mycological-films is included. (Author/SA) *********************************************************************** * Reproductions supplied by EDRS are the best that can be made * * from the original document. * *********************************************************************** GE e75% Mycology Guidebook Mycology Guidebook Committee, -
Cytology of Teliospore Germination and Basidiospore Formation in Uromyces Appendiculatus Var
erotoplasma 119, 150- 155 (1984) I~O'['OPt~ by Springer-Verlag 1984 Cytology of Teliospore Germination and Basidiospore Formation in Uromyces appendiculatus var. appendiculatus 1 R. E. GOLD* and K. MZNDGEN** Lehrstuhl fiir Phytopathologie, Fakultiit fiir Biologie, Universit~it Konstanz Received May 11, 1983 Accepted in revised form June I I, 1983 Summary (e.g., AI.I~ZN 1933, PAVGI 1975, AN~KSTZR et al. 1980). The cytology of teliospore germination and basidiospore formation These studies have revealed the extremely variable in Urornyces appendiculatus vat. appendieulatus was characterized pattern of meiotic development in the rusts. with light and fluoroscence microscopy. Meiosis of the diploid The purpose of the present study was to provide, for the nucleus occurred in the metabasidium. The four haploid daughter first time in Uromyces appendiculatus var. nuclei migrated into the basidiospore initials where they underwent a appendiculatus, qualitative and quantitative cytological post meiotic mitosis. Each basidiospore was delimited from the meatabasidium by a septum at the apex of the sterigrna. Seventy-five information on the processes of teliospore germination percent of mature basidiospores were binucleate, 24.5% uulnucleate, and basidiospore formation. and 0.5% trinucleate. Mature, released basidiospores measured 16 x 9 gin, were smooth-surfaced, and reniform to ovate-elliptical 2. Materials and Methods in shape. The isolate of Uromyces appendiculatus (Pers.) Unger vat. Keywords: Basidiospore; Cytology; Meiosis; Teliospore germination; appendiculatusz used in this study was collected in the field as Uromyces appendiculatus. urediniospores from infected leaves of Phaseolus vulgaris L. The collection was made in the Black Forest (Lahr Valley) in August, 1978. Teliospores were produced, stored, and activated to germinate according to methods described earlier (GOLD 1983, GOLD and 1. -
Mlab 1331: Mycology Lecture Guide
MLAB 1331: MYCOLOGY LECTURE GUIDE I. OVERVIEW OF MYCOLOGY A. Importance of mycology 1. Introduction Mycology - the study of fungi Fungi - molds and yeasts Molds - exhibit filamentous type of growth Yeasts - pasty or mucoid form of fungal growth 50,000 + valid species; some have more than one name due to minor variations in size, color, host relationship, or geographic distribution 2. General considerations Fungi stain gram positive, and require oxygen to survive Fungi are eukaryotic, containing a nucleus bound by a membrane, endoplasmic reticulum, and mitochondria. (Bacteria are prokaryotes and do not contain these structures.) Fungi are heterotrophic like animals and most bacteria; they require organic nutrients as a source of energy. (Plants are autotrophic.) Fungi are dependent upon enzymes systems to derive energy from organic substrates - saprophytes - live on dead organic matter - parasites - live on living organisms Fungi are essential in recycling of elements, especially carbon. 3. Role of fungi in the economy a. Industrial uses of fungi (1) Mushrooms (Class Basidiomycetes) Truffles (Class Ascomycetes) (2) Natural food supply for wild animals (3) Yeast as food supplement, supplies vitamins (4) Penicillium - ripens cheese, adds flavor - Roquefort, etc. (5) Fungi used to alter texture, improve flavor of natural and processed foods b. Fermentation (1) Fruit juices (ethyl alcohol) (2) Saccharomyces cerevisiae - brewer's and baker's yeast. (3) Fermentation of industrial alcohol, fats, proteins, acids, etc. Mycology.doc 1 of 25 c. Antibiotics First observed by Fleming; noted suppression of bacteria by a contaminating fungus of a culture plate. d. Plant pathology Most plant diseases are caused by fungi e. -
Basidiomycota (Club Fungi) the Basidiomycota (Colloquially Basidiomycetes) Are a Large Group of Fungi with Over 30 000 Species
B.Sc Botany (Hons) Semester II Mycology and Phytopathology Basidiomycota (club fungi) The Basidiomycota (colloquially basidiomycetes) are a large group of fungi with over 30 000 species. They include many familiar mushrooms and toadstools, bracket fungi, puffballs, earth balls, earth stars, stinkhorns, false truffles, jelly fungi and some less familiar forms. Also classified here are the rust and smut fungi, which are pathogens of higher plants and may cause serious crop diseases. Most basidiomycetes are terrestrial with wind-dispersed spores, but some grow in freshwater or marine habitats. Many are saprotrophic and are involved in litter and wood decay, but there are also pathogens of trees. the honey fungus, Armillaria, which attacks numerous tree species, and Heterobasidion annosum, which can seriously damage conifer plantations. 1. The somatic phase consists of a well developed, septate, filamentous mycelium which passes chiefly through two stages: a) primary mycelium- it is formed by the germination of a basidiospore and contains a single haploid nucleus in each cell. It bears neither sex organs nor any basidia and basidiospores. It is short lived b) Secondary or dikaryotic mycelium It constitutes the main food absorbing phase and consists of cells each containing two haploid nuclie. It is long lived and plays prominent role in the life cycle. In Homobasidiomycetidae it may continue to grow for years producing fructifications every year by the interweaving of hyphae. The fructifications bear basidia and basidiospores In Heterobasidiomycetidae it forms teleutospores or brand spores which germinate to produce basidia bearing basidiospores. 2.Except in rusts and smuts the septal pore in the Basidiomycetes is complex, It is dolipore septa with parenthesome type. -
Fungi Topics in Biodiversity
Topics in Biodiversity The Encyclopedia of Life is an unprecedented effort to gather scientific knowledge about all life on earth- multimedia, information, facts, and more. Learn more at eol.org. Fungi Author: University of Guelph (Content) Editor: Sidney Draggan Source: Encyclopedia of Earth Photo credit: Russula by Vanessa Ryan, Flickr: EOL Images. CC BY-NC-SA Introduction The word fungus usually evokes images of mushrooms and toadstools. Although mushrooms are fungi, the forms that a fungus may take are varied. There are over 100,000 species of described fungi and probably over 200,000 undescribed. Most fungi are terrestrial, but they can be found in every habitat worldwide, including marine (500 spp.) and freshwater environments. Fungi are nonmotile, filamentous eukaryotes that lack plastids and photosynthetic pigments. The majority of fungi are saprophytes; they obtain nutrients from dead organic matter. Other fungi survive as parasitic decomposers, absorbing their food, in solution, through their cell walls. Most fungi live on the substrate upon which they feed. Numerous hyphae penetrate the wood, cheese, soil, or flesh in which they are growing. The hyphae secrete digestive enzymes that break down the substrate, enabling the fungus to absorb the nutrients contained within the substrate. There are four major groups of fungi: Zygomycota, Ascomycota (sac fungi), Basidiomycota (club fungi), and Deuteromycota (fungi imperfecti). 1 Zygomycota The fungal group Zygomycota is most frequently encountered as common bread molds, although both freshwater and marine species exist. Most of these live on decaying plant and animal matter found on the substrate. Aquatic species are primarily found in sediments or on algae, but some species are also free floating.