Recovery of Fermentation Products Engineering Foundation Conference Co-Chairs Charles L

Recovery of Fermentation Products Engineering Foundation Conference Co-chairs Charles L. Cooney, Massachusetts Institute of Technology, USA Kenneth G. Taksen, Pfizer, Inc., USA

The Cloister, Sea Island, Georgia, USA 29 January - 3 February, 1984

Sunday, 29 January Tuesday, 31 January

Plenary Lectures Purification Methods: Chromatography and Separations Technology: Achilles Heel of the Adsorption Bioprocess Industries Session Chairs E. Gaden, University of Virginia, USA Saxena, Bio Rad Laboratories, USA Innovation in Separations: Key to Success in M. Ladisch, Purdue University, USA Adapting Modern Biology to Industrial Practice Scale-Up Considerations in Process HPLC A. Michaels, USA V Saxena, BioRad Laboratories, USA Purification of Pharmaceutical Compounds by Monday, 30 January Process HPLC W. Skea, Millipore Corp., USA Cell Disruption and Harvesting Scale-up of Affinity Chromatography Session Chairs H. W. Blanch, University of California, USA J. Sinskey, Massachusetts Institute of Technology, USA Protein Recovery from Unclarified Cell Homogenates C.G. Rosen, Alfa Laval, Sweden P. Hedman, Pharmacia Fine Chemicals, Sweden Centrifugal Separation in Biotechnology Selective Recovery of Polar Organics by Solvent A. Larsson, Alfa Laval Separation, Sweden Regenerated Adsorptive Processes Genetic Solutions to Cell Disruption Problems C. J. King, University of California, USA A. J. Sinskey, Massachusetts Institute of Technology, Column Design Factors in Aqueous Liquid USA Chromatography of Fermentation Substrates and Production and Reaction Kinetics of Microbial Cell- Products Lytic Enzyme Systems M. R. Ladisch, Purdue University, USA J. Asenjo, B. A. Andrews and J. B. Hunter, Columbia University, USA Purification Methods: Extraction Mechanical Disruption of Cells Session chair C. Engler, Texas A & M University, USA J. King, University of California, USA Strategy, Tactics and Constraints in Applying Liquid- Purification Methods: Membrane Process Liquid Extraction to the Biotechnology Field Session chair R. Blumberg, Miles Laboratories, Israel R.Tutunjian, Amicon Corp., USA Continuous Extraction of Enzymes in Aqueous Phase Systems Evaluation of Current Ultrafiltration Membranes in M -R. Kula, Gesellschaft für Biotechnologische Biotechnology Forschung, Germany W. C. McGregor, Hoffman-La Roche, Inc., USA Ion-Pair Extraction of Penicillin Cross Flow Filtration for Protein Recovery K. Schugerl, University of Hannover, Germany K.H. Kroner, Gesellschaft für Biotechnologische Forschung, Germany Super Critical Fluid Extraction: State of the Art R. Reid, Massachusetts Institute of Technology, USA Recovery of an Extracellular Antibiotic by Ultrafiltration Super Critical Fluid Extraction of Lipids from D.P. Gravatt and T. E. Molnar, Merck & Co., USA Fermentation Products V. Krukonis, Phasex, USA

Wednesday, 1 February Integrated Fermentation and Recovery in Membrane Entrapped Cell Cultures by M. L. Shuler, Cornell University, USA Purification Methods: Electrokinetic Plenary Lecture Session Chairs P. B. Reed, Ionics, Inc., USA Interactions between Fermentation and Downstream A. Ramel, Genentech, USA Protein Processing M. Lilly and M. M. Fish, University College, London, UK Electrodialysis in the Separation of Chemicals W. A. McRae, Ionics, Inc., USA The Use of Electrodialysis and Recycling Isoelectric Friday, 3 February Focusing for the Purification of Proteins including Integration of Fermentation and Recovery Interferon Systems T.L. Nagabhushan, Schering Corp., USA Session Chairs New Electrophoretic Methods that give High Capacity W. Wernau, Pfizer, USA Equilibrium Separation H. Blanch, University of California, Berkeley, USA P. O’Farrell, University of California, USA Fermentation Process Integration - A New Approach Electrical Purification of Glutathione and A.R. Thomson, Harwell, UK Electrochemical Reduction of Oxidized Glutathione Y. Yokoo and S. Noguchi, Kyowa Hakko, Japan Integration of Fermentation and Separation through Advances in Electrophoretic Separations Two-Phase Aqueous and Membrane Systems R.S. Snyder, NASA, USA B.Mattiason, University of Lund, Sweden Enzyme Production with Insoluble Substrate Recycle Workshop Sessions on Economics and J. Berke, Institute for Biotechnology, Germany Scale-up Continuous Hybridoma Growth and Monoclonal Antibody Production in a Hollow Fiber Reactor Cell Harvesting Georges Belfort, Rensselaer Polytechnic Institute, USA D.I. C. Wang, Massachusetts Institute of Technology, USA Selective Lactose Crystallization from Lactose, Fructose and Galactose Syrups in the Enzymatic Cell Disruption Hydrolysis of Whey M.-R. Kula, Gesellschaft für Biotechnologische J. Prenosil, ETH Zurich, Switzerland Forschung, Germany Membrane Processes Poster Session M. Keyes, Pfizer, Inc., USA Session Chair Chromatography W. -S. Hu, University of Minnesota, USA. S. Builder, Genentech, Inc., USA The Rapid Isolation of Protein from Complex Mixtures Gary J. Calton, Purification Engineering, Inc., USA Thursday, 2 February Continuous Flow Membrane Plasmapheresis Purification Methods: Theoretical Models for Flux & Hemolysis Prediction Clark K. Colton and Andrew L. Zydney, Massachusetts Precipitation/Crystallization Institute of Technology, USA Improvement in Process Design for Protein Protein Recovery from Lysates Using Tangential Flow Purification by Precipitation Filtration M. Hoare and P. Dunhill, University College, London, UK Raymond Gabler and Mary Ryan, Millipore Corporation, USA Integration of Fermentation and Recovery Primary Particle Formation in Protein Precipitation Systems Clark Nelson and Charles Glatz, Iowa State University, USA Session Chairs W. Wernau, Pfizer, USA Purification of Biosynthetic Human Insulin from E. H. Blanch, University of California, USA Coli P. Tocher, J. Cunningham, W. Bradley, S.A. Cockle and Membrane Bioreactors E.James, Connaught Research Institute, Canada J. Quinn, University of Pennsylvania, USA Extraction of Insulin-Related Material from Process Improvement through Integrating Prokaryotes Fermentation with Extraction Processes Chaim Rubinovitz and Joseph Shiloach, National H. Y. Wang, University of Michigan, USA Institutes of Health, USA By-Product Effects on Fermentation Recycle Systems B.L. Maiorella, University of California, USA

Purification Process Development Using HPLC and Mass Balance Computer Programs M. Thille, Rhône-Poulenc Inc., USA Preliminary Investigation on an Enzyme Recovery Process Based on the Principles of the Liquid Membrane Process K. Van T Riet, M. Dekker, Wageningen Agricultural University, The Netherlands Improvements in Process Design for Protein Purification by Precipitation M. Hoare and P. Dunnill, University College London, UK Membrane Bioreactors John A. Quinn, University of Pennsylvania, USA Process Improvement through Integrating Fermentation with Extraction Processes Henry Y. Wang, The University of Michigan, USA Inhibitor Accumulation and Toxic Effects in Integrated Ethanol Fermentation and Recovery Processes B. L. Maiorella, H.W. Blanch and C.R. Wilke, University of California, USA Integration of Fermentation and Recovery in Membrane Entrapped Cell Cultures M. L. Shuler, P.A. Schamel, J.A. Grzyb, and T. Cho, Cornell University, USA Adsorption Equilibrium of Lysine on the Ion Exchange Resin Tetsuya Kawakita, Hiromasa Maruyama and Tsutomu Matsuishi, Cornell University, USA Recovery of the Immunosuppressant Cyclosporin A from Trichoderma Polysporum Argyrios Margaritis, Martin Rieger, and Jeff Tucker, The University of Western Ontario, Canada Membrane Processes for Fermentation Product Recovery — Reverse Osmosis and Coupled Transport Stephen L. Matson, Bend Research, Inc., USA Modeling and Sizing of an Adsorption Column B. Siret, Rhône- Poulenc Inc., USA Ethanol Recovery by Solvent Extraction D.W. Tedder. A. J. Eckles, P. J. Ferster, W. Y. Tawfik and A. S. Myerson, Georgia Institute of Technology, USA Membrane Dehydration of Azeotropic Ethanol/Water Vapor Mary K. Tripodi, Monsanto Company, USA Survey of Isolation Methods of Antibiotics from Complex Media - As Illustrated Ceph C Marvin Wildfeuer, Eli Lilly and Company, USA