On the Genus Ditylenchus Filipjev, 1936 (Nematoda : Tylenchida)
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On the genus Dilylenchus Filipjev, 1936 (Nernatoda : Tylenchida) Renaud FORTUNER* Laboratoire de Nématologie, ORSTOM, BP V-51, Abidjan, Côte d’Ivoire. SUMMARY. Several populations of Ditylenchus myceliophagus from Ivory Coast and Upper-Volta were studied for variability of generic and specific taxonomie characters, which was found to be greater than generally accepted.Ditylenchus is redefined ; Chitinotylenchus is considered genus inquirendum; Diptenchus and Safianema are synonymized with Ditylenchus ; Pseudhalenchus is considered a valid genus under Tylenchinae; Pseudhalenchinae is synonymized with Xylenchinae. Ditylenchus geraerti is synonymized with D. myceliophagus. The following new combinations are pro- posed : Ditylenchus indicus for Pseudhalenchus indicus; Ditylenchus acutus for Pseudhalenchus acutus; Ditylenchus khani for Diptenchus indicus; Ditylenchus lutonensis for Safianema lutonense; D. anchilisposomus for S. anchilispo- somum; D. damnatusfor S. damnatum; S. hylobii is transferred backto Pseudhalenchus. Four species are considered species inquirendae :Ditylenchus bacillifer, D. intermedius,D. humuli and D. karakalpakensis. Chitinotylenchus para- gracilis, is considered species incertae sedis. A tabular keyto the validspecies of Ditylenchus is presented. RÉSUMÉ Le genre Ditylenchus Filipjev, 1936 (Nematoda : Tylenchida) Plusieurs populations deDitylenchus myceliophagus de Côte d’Ivoire et de Haute-Volta ont été étudiées; la varia- bilité des caractkres taxonomiques utilisés aux niveaux générique etspécifique s’est montrée plus grande quegéné- ralement admis. Ditylenchus est redéfini ; Chitinotylenchus est considéré comme genus inquirendum; Diptenchus et Safianema sont synonymisés avecDitylenchus; Pseudhalenchus est considéré comme un genre valideappartenant aux Tylenchinae ; la sous-famille des Pseudhalenchinae est synonymisée avec celle des Tylenchinae.Ditylenchus geraerti est synonymisé avec D. myceliophagus. Les combinaisons nouvelles suivantes sont proposées : Ditylenchus indicus pour Pseudhalenchus indicus; Ditylenchus acutus pour Pseudhalenchus acutus; Ditylenchus khani pour Diptenchus indicus; Ditylenchus lutonensis pour Sa fianema lutonense; D.anchilisposomus pour S. anchilisposomum; D. damnatus for S.darnnatum; S. hylobii est retransféré à Pseudhalenchus. Quatre espèces sont considérées comme species inqui- rendae : Ditylenchus bacillifer, D. intermedius, D. humuli et D. karakalpakensis. Chitinotylenchus paragracilis est considéré species incertae sedis. Une clé tabulaireest présentée pour la détermination desesphces valides de Ditylenchus. Nematodes of the genus Ditylenchus were variable and only a few were constant enough observedfrom diseased ricepanicles inthe to be used for taxonomic purposes. A search of Ivory Coast and it was thought, at first, that the literature revealed that similar variability they might be responsiblefor the poor condition exists in atleast several other species of the of the rice. However, a study of their morphol- genus. ogypermitted their identification as D. Inyce- This led the ahthor to question the validity Ziiphagus Goodey, 1958, a well knownfungus of the species described under Ditylenchus and feeder. also of the genera closely related to this taxon. Duringthese studies, it was observed that As norecent review of thegenus has been manymorphometrical characters were highly published, a tabular Bey to the valid species in * Present Address : Department of Food and Agriculture, Laboratory Services/Nema Lab., 1220 N Street, Sacra- mento, California 95814, U.S.A. Revue Nématol. 5 (1) : 17-38 (1982) 17 R. Fortuner Ditylenchus was constructed,using only the Theobservations were made using a Leitz reliablecharacters. Brzeski (1981) recently Ortholux II microscope at 1 O00 x magnifica- proposeda revised classification of thefamily tion,in bright field microscopyor with an Anguinidae(including Ditylenchus) and his interferencecontrast device of Nomarski. For article was used as a framework forthe present measurement purposes, drawings were made of study. al1 specimens using a drawing attachment or a AnguininaeParamonov, 1962was raised to cameralucida, both systems set to give an familyrank by Siddiqi (1971) andto super- additional enlargement factor of two. family rank by Siddiqi(1980b). The status of thisgroup will not bediscussed here andits lowest rank (Anguininae) 'will be used. Results OBSERVATIONSMADE ON THE SPECIMENS Materials and Methods Table 1 (females) presents,for the four samples observed,the body length, stylet length, dis- Thestudied specimens were obtainedfrom tancefrom anterior end to hemizonid and to the localitiesfollowing : , excretorypore, length of oesophagus (tothe Sample 1 - Thirty females and twenty males oesophage-intestinal junction and to the end of fromupland ricepanicles (cv.Iguape Cateto) glands),length of tail,anal and vulval body sent in1976 by A. Pouzet (ORSTOM agronomist) from A.V.B. (Autorité pour l'Aménagement de diameters, distance from head to vulva, length of anterior genital branch and of post uterine la Vallée duBandama) fields atFitabro 1 sac(P.U.S.), and distance between vulva and (bloc C, plot 7.04), in the Ivory Coast. anus. Table 2 presents the same characters for Sample 2 - Sixindividuals from sample 1 were inoculated into a test tube containing the males in samples 1, 2 and 4, with bursa, testis, spiculeand gubernaculum lengths in place of fungus Colletotrichum gloeosporioides Penz. on the female genital characters. For every charac- potato-dextroseagar. After three weeks, the ter, the meanis given with itsconfidence interval tube wasfound ta contain 120specimens of Ditylenchus of whichfifteen females and eight (i = $ ) therange and the coeRcient of males were selected for morphological studies. Sample 3 - Six females from an A.V.B. field variability. at Assakra 1, a village close to Fitabro 1. Figure 1 presents graphically the correlations Sample 4 - Nine females and five males from betweenthe constituents of the usualratios soil aroundroots of sugarmne at Banfora (a, b, c, c', and V) and post-uterine sac length (Upper-Volt,a) in the SOSUHV (Société Sucrière in relation to vulva-anus distancefor the females de Haute-Volta) fieldscollected by P. Cadet. in samples 1 + 2. Some morphological features Twofemales and one male were also observed are illustrated in Figures 2 and 3. fromforest soilfrom Taïforest, in the Ivory Coast. DISCUSSIONON THE VARIABILITY OF SOME, The specimens wereBilled by FP 4:l (Netscher MORPHOMETRICAL CHARACTERS & Seinhorst, 1969), fixed in 4% formaldehyde and mounted in glycerin on Cobb slides. Orcein General morphology wasadded during the mounting process for coloring the nematodes. A few individuals from Habitus. In the present specimens, the body sample 2 were observed in pure water, just after was almost straight or slighly C-shaped, usually beingkilled bygentle heat, ta observesome wit.h a bend located in the vicinity of the vulva inconspicuousfeatures (median oesophageal (Fig. 2 A, B). bulb valve for instance). Some females were also Body length. Thebody length was highly observed in cross-section in glycerin. variable (Tab.1) ; it ranged from 600to 1 200 prn -. -. - . - __ .- . - .~. - - ~ - . - _.. _- 18 Revue Nématol. 5 (1) : 17-38 (1982) Genus Ditylenchus . ... 1001 I.1 500 y = 13 X +41 r = 0.7730 - 5'0 9'0 T c VA1 1OOC 100 500 50 Db 20 r = 0.7833 Fig. 1. Ditylenchus myceliophagus. Graphic representation of ratios a, b, c, CI, V, and post-uterine sac in relation to vulva-anus distance andto body diameter. L : body length ; Dv : body diameter at levelof vulva ; œ : length of oesophagus ; T : tail length ; HV : head to vulva distance ; VAN : vulva to anus distance; Da : body diameter at level of anus ; PUS : length of post-uterine sac ; y = f(x): equation of the regression lines ; r = coescient of correlation (n = 45 ; r significant above 0.380 at 1y0 level). in sample 1, 700 to 1 400 pm in sample 4, with size might also be caused by different ecological meanvalues of 800-850 pm. Sample 3 was environment,individuals fromrainforest (Taï) smaller,with a mean value of only 546 Pm. being slnaller (400 and 575 pm) than those from Brzeski (1967) andEvans and Fisher (1970) have savanna regions (A.V.B. fields and Upper-Volta, shown that the body lengtb of D.rnyceliophagus samples 1 to 4). depends on the food supply and the nature of Bodydiameter. Thebody diameter also had thefungus on wbich it feeds.Hesling (1974) ahigh variability in the present specimens gave a range for body length inD. myceliophagus (Tab. 1).Brzeski (1967) observed no statistical of 600-1 380 Pm.Goodey (1952), Thorneand difference in width in specimens of D. mycelio- Allen (1959), and Wu (1960b) observed that in plzagus but itshould be noted that he measured D. destructor thebody length varies greatly this character only at pharynx and anus levels. depending on the plant host. Differences in body Goodey (1952), whileobserving thatin, D. Revue Nématol. Revue 5 (1) : 17-38 (1982) 19 R. Fortuner Revue Ném.atol. 5 (1) : 17-38 (1992) Genus Ditylenchus destrucfor generallythe longer the worm, the specimens, almost invisible in most of the body, fatter it is, reported some variation related to more marked just below the lip region. thehost. A population from mint was of the Phasmids. Phasmids were not observed in the same length but much slimmer than a popula- present specimens asis generally the case in tion from potato. Difylenchus and the Anguininae. Phasmids were Ratio“a”. Thecorrelation of thetwo con- reportedin D. nortorzi andin the