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Iotest CD109-PE Conjugated Antibody

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Iotest CD109-PE Conjugated Antibody IOTest® CD109-PE PN A08933 – 100 tests –Liquid - 20 µL / test - Clone 8A3 For Research Use Only. Not For Use In Diagnostic Procedures. SPECIFICITY PN A08933 – 100 tests – Liquid - 20 µL / test STORAGE CONDITIONS AND STABILITY The CD109 antigen is a monomeric Clone 8A3 This reagent is stable up to the expiration glycosylphosphatidylinositol (GPI)-linked Isotype IgG2a, k, mouse date when stored at 2 – 8° C. Do not freeze. glycoprotein of 170 kDa under both reducing Immunogen KG1-a cell line Minimize exposure to light. and non-reducing conditions. It contains Hybridoma SP2/0 x Balb/c several N-linked endoglycosidase H-sensitive Source Ascites fluid EVIDENCE OF DETERIORATION hybrid-type glycans but no O-linked glycan Purification Ion exchange or affinity Any change in the physical appearance of (1). It has been reported as a novel member chromatography this PE-labeled reagent (clear, colorless to of the alpha 2 macroglobulin (alpha2M) / C3, Conjugation R-phycoerythrin (PE) pink liquid) or any major variation in values C4, C5 family of thioester-containing proteins Molar Ratio PE / Ig : 0.5 – 1.5 obtained for control samples may indicate (2). Fluorescence Excites at 488 nm deterioration and the reagent should not be The CD109 antigen is found on vascular Emits at 575 nm used. endothelial cells, some epithelial cells, activated, but not resting, T-cells, activated, Main emission color: Orange-red REAGENT PREPARATION but not resting, platelets, leukemic No reconstitution is necessary. This megakaryoblasts and a subset of bone REAGENT CONTENTS monoclonal antibody may be used directly marrow CD34+ cells (1, 3). This antigen is not This antibody is provided in phosphate from the vial. Bring reagent to 18 – 25° C expressed on fresh peripheral blood buffered saline pH 7.4, containing 0.1% prior to use. lymphocytes (PBL) (4, 1). Weak binding is sodium azide and 2 mg/mL bovine serum detectable in peripheral blood monocytes. albumin. Human umbilical vein endothelial cells PROCEDURE This reagent is designed for flow cytometry. (HUVEC) and some endothelial cell lines like 5 EA.hy 926 have a basal level of CD109 APPLICATION Assay volume: 20 µL per 5 x 10 cells in one antigen expression (5). Poorly differentiated This conjugated monoclonal antibody is test, or per 100 µL whole blood. (CD34+, TdT+, CD7+) T-acute leukemias and designed for flow cytometry studies of CD109 A wash is required to yield optimal results. rare cases of chronic myeloid leukemia in expressing cells (e.g. activated T cells, + megakaryoblast crisis expressed the CD109 endothelial cells, CD34 HPC and EXAMPLE DATA antigen. Furthermore, megakaryoblastoid cell megakaryocytic cells). The histogram below is a monoparametric lines (MO7e, MOLM-1) are CD109+ (4). representation (Count versus Fluorescence The CD109 antigen, that is strongly STATEMENT OF WARNINGS Intensity) of a fresh HUVEC cell line. Staining expressed on the KG1a cell line with 20 000 1. This reagent contains 0.1% sodium is with CD109-PE monoclonal antibody (PN binding sites per cell (6), may represent a azide. Sodium azide under acid A08933). The isotypic control labeling very early marker for hematopoietic cells conditions yields hydrazoic acid, an (IgG2a-PE; PN A09141) is shown in light. committed to the megakaryocyte lineage as extremely toxic compound. Azide demonstrated by studies on fetal bone compounds should be flushed with Acquisition is with a COULTER® EPICS® + + marrow where the CD34 CD109 subset running water while being discarded. XL™ flow cytometer. Analysis is with the identifies almost all myelo-erythroid and These precautions are recommended to CYTOMICS™ RXP™ software. megakaryocytic progenitors (7). By contrast, avoid deposits in metal piping in which + + the adult bone marrow CD34 CD109 explosive conditions can develop. If skin subset identifies the most primitive or eye contact occurs, wash excessively hematopoietic stem cells capable of long- with water. term culture and lymphoid progenitors of the 2. Specimens, samples and all material B-cell lineage (7, 3). coming in contact with them should be In human platelets, the tyrosine 703 serine handled as if capable of transmitting polymorphism that defines the Gov a/b infection and disposed of with proper alloantigens is localized on the CD109 precautions. molecule (8, 9). 3. Never pipet by mouth and avoid contact Structural and serologic characteristics of the of samples with skin and mucous CD109 antigen indicate that it is different membranes from other leukocyte activation antigens 4. Do not use antibody beyond the including transferrin receptors, interleukin-2 expiration date on the label. receptors, HLA class II molecules and from 5. Do not expose reagents to strong light platelet activation-specific molecules like the during storage or incubation. activated form of GPIIb/IIIa and GMP140 (1). 6. Avoid microbial contamination of The 8A3 monoclonal antibody was first reagents or incorrect results might The histogram below is a monoparametric assigned to the CDw109 cluster of occur. representation (Count versus Fluorescence differentiation at the 5th International 7 Use good laboratory practises when Intensity) of KG1a cell line. Staining is with Workshop on Human Leukocyte handling this reagent. Differentiation Antigens (HLDA) in Boston, CD109-PE monoclonal antibody (PN USA (6) and reassigned to CD109 at the 6th A08933). The isotypic control (PN A09141) International HLDA Workshop in Kobe, Japan labeling is shown in light. (1996) (WS Code: E010, section E ) (4) . Acquisition is with a COULTER® EPICS® XL™ flow cytometer. Analysis is with the CYTOMICS™ RXP analysis software. REAGENT IOTest CD109-PE Conjugated Antibody 1/2 I-A08933 2008-03-04 IOTest® CD109-PE PN A08933 – 100 tests –Liquid - 20 µL / test - Clone 8A3 Alitalo, K., Ullrich, A., Kanz, L., Bühring, Sutherland, D., R., Metcalfe, P., H.J., "Functional and phenotypic Horsfall, W., Ouwehand, W., H., "A characterization of cord blood and bone tyrosine 703serine polymorphism of marrow subsets expressing FLT3 CD109 defines the Gov platelet (CD135) receptor tyrosine kinase", alloantigens", 2002, Blood, 5, 99, 1692- 1997, Blood, 1, 90, 111-125. 1698. 4. Sutherland, D., R., Yeo, E., L., "EC4 CD109 Workshop panel report", TRADEMARKS AND PATENTS Leucocyte Typing VI, 714-716. Beckman Coulter, the Beckman Coulter logo, 5. Mutin, M., Dignat-George, F., Sampol, COULTER, EPICS, XL, CYTOMICS, RXP J., "Immunologic phenotype of cultured and IOTest are registered trademarks of endothelial cells: Quantitative analysis Beckman Coulter, Inc. of cell surface molecules", 1997, Tissue Antigens, 5, 50, 449-458. 6. Sutherland, D., R., Yeo, E., L.,"CDw109 PE is licensed under patent 4,520,110 cluster report", 1995, Leucocyte Typing V, Ed. S. F. Schlossman et al., 1767- 1769. MANUFACTURED BY: 7. Murray, L., J., Bruno, E., Uchida, N., Immunotech SAS, SELECTED RESEARCH REFERENCES Hoffman, R., Nayar, R., Yeo, E., L., A Beckman Coulter Company 1. Sutherland, D., R., Yeo, E., Rian, A., Schuh, A., C., Sutherland, D., R., 130, avenue de Lattre de Tassigny, B.P. 177 Mills, G., B., Bailey, D., Baker, M., A., "CD109 is expressed on a 13276 Marseille Cedex 9, France "Identification of a cell-surface antigen subpopulation of CD34+ cells enriched associated with activated T in hematopoietic stem and progenitor For additional information in the USA, call lymphoblasts and activated platelets", cells", 1999, Exp. Hematol., 8, 27, 1282- 800-526-7694. 1991, Blood, 1, 77, 84-93. 1294. Outside the USA, contact your local 2. Lin, M., Sutherland, D., R., Horsfall, W., 8. Smith, J., W., Hayward, P., M., Beckman Coulter representative. Totty, N., Yeo, E., Nayar, R., Wu, X., F., Horsewood, P., Warkentin, T., E., Schuh, A., C., "Cell surface antigen Denomme, G., A., Kelton, J., G., www.beckmancoulter.com CD109 is a novel member of the alpha "Characterization and localization of the (2) macroglobulin/C3, C4, C5 family of Gov a/b alloantigens to the Printed in France thioester-containing proteins", 2002, glycosylphosphatidylinositol-anchored Blood, 5, 99, 1683-1691. protein CDw109 on human platelets", ©2008 Beckman Coulter, Inc. 3. Rappold, I., Ziegler, B.L., Köhler, I., 1995, Blood, 7, 86, 2807-2814. All Rights Reserved Marchetto, S., Rosnet, O., Birnbaum, D., 9. Schuh, A., C., Watkins, N., Simmons, P.J., Zannettino, A.C.W., Hill, A., Nguyen, Q., Harmer, N., J., Lin, M., B., Neu, S., Knapp, W., Alitalo, R., Prosper, J., Y., Campbell, K., 2/2 I-A08933 2008-03-04 .
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