bioRxiv preprint doi: https://doi.org/10.1101/035543; this version posted May 3, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Identification and qualification of 500 nuclear, single-copy, orthologous genes for the 2 Eupulmonata (Gastropoda) using transcriptome sequencing and exon-capture 3 Luisa C. Teasdale 1, 2, Frank Köhler3, Kevin D. Murray4, Tim O’Hara1, and Adnan Moussalli1 4 5 1 Sciences Department, Museum Victoria, 11 Nicholson St, Carlton, Vic, Australia 3053 6 2 School of BioSciences, The University of Melbourne, Parkville, Vic, Australia 3010 7 3 Australian Museum, 6 College Street, Sydney, NSW, Australia 2010 8 4 Division of Plant Sciences, Research School of Biology, Australian National University, 9 Australia 0200 10 11 Corresponding author: Luisa Teasdale, Mailing address: Sciences Department, Museum 12 Victoria, 11 Nicholson St, Carlton, Vic, Australia 3053, Fax Number: + 61 3 8341 7442, 13 Email:
[email protected] 14 15 Key words: orthology, single-copy, phylogenomics, Pulmonata, transcriptome, targeted 16 enrichment 17 Running header: Orthologous genes for Eupulmonata 18 19 20 ABSTRACT 1 bioRxiv preprint doi: https://doi.org/10.1101/035543; this version posted May 3, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 21 The qualification of orthology is a significant challenge when developing large, multi- 22 loci phylogenetic datasets from assembled transcripts.