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Krosflo Hollow Fibers Ultra/Filtration Modules

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Krosflo Hollow Fibers Ultra/Filtration Modules KrosFlo Hollow Fibers Ultra/Filtration Modules Tangential Flow Filtration (TFF) with hollow fiber modules facilitate the direct scalability of ultrafiltration and microfiltration applications (desalting, concentration, filtration, clarifications) for R&D to productions with volumes ranging from a few milliliters to thousands of liters. Watch a video high quality hollow fiber membranes encased in a symmetrical polysulfone housing designed to tangential flow filtration available in a variety for size, for scaling for R&D to Pilot and Production levels operated by hand or a peristaltic pump for small units, or by larger systems (KrosFlo Research to Pilot and Pharma) Here is a selection guide by applications, and an overview of hollow fiber modules series, followed by Basics of Filtration/UltraFiltration, Advantages of our Fiber technology, versus Others, hollow microfiber material information and Chemical Compatibility. Selection guide Application Membrane MWCO Membrane Material Concentrate proteins, peptides, enzymes, recombinant 1, 3, 5, 10, 30, 50, (mPES) Modified Polyethersulfone proteins, and/or diafiltration (Buffer Exchange) of 70,100kD or (PS) Polysulfone these products Depyrogenation Water / Buffers 3, 10kD (mPES) Modified Polyethersulfone or (PS) Polysulfone Nano-Particle Coating, Buffer Exchange, Fractionating 70,100,300,500,750kD, (mPES) Modified Polyethersulfone and/or Dewatering 0.2µm or (PS) Polysulfone Concentrate and diafilter albumin (nominal MW 67kd) 10kD (mPES) Modified Polyethersulfone Concentration of monoclonal antibodies (155kd) and 30kD (mPES) Modified Polyethersulfone Buffer Exchange Envelope and Non-Envelope Virus Concentration and 50, 70, 100, 300, 500, (mPES) Modified Polyethersulfone Diafiltration 750kD, 0.05µm or (PS) Polysulfone Clarification of cell lysates, viral expressed cultures to 500, 750kD, 0.05, (mPES) Modified Polyethersulfone, recover product in permeate 0.1µm (PS) Polysulfone, or (ME) Mixed Ester Concentration and washing of Insect, Bacterial and/or 750kD, 0.1, 0.2, (mPES) Modified Polyethersulfone mammalian cells 0.65µm or (ME) Mixed Ester Continuous Bioreactor Perfusion of cell culture 30, 50, 70, 100, 300, (mPES) Modified Polyethersulfone 500, 750kD, 0.2, or (PS) Polysulfone 0.65µm SS (single stranded) or DS (double stranded) Nucleic 1, 3, 5, 10, 30, 50, (mPES) Modified Polyethersulfone Acids 70,100kD Washing Latex, Polystyrene and other diagnostic 500, 750kD, 0.05, 0.1, (mPES) Modified Polyethersulfone, particles to remove un-reactives / unbound drug subst. 0.2, 0.65µm (PS) Polysulfone, or (ME) Mixed Ester Overview of KrosFlow modules series Hollow Fiber modules Process scale MWCO Membrane type Inlet/outlet Volumes Fiber ID Connection Surface area Packaginf Systems (overview) Lenght of use MicroKros R&D 10kD, PS, PES, ME MLL 50kD, FLL 100kD, 2ml - 50ml 400kD, 1,0mm, 0.6mm, 0.5mm 500kD*, Research II System 8 - 20 cm2 0.05µm*, Dry, Irradiated, Pre-wetted 0.1µm, 0.2µm, Disposable 0.5µm More information MidiKros R&D 10kD, PS, PES, ME 1/4" HB 50kD, FLL 100kD, 20ml - 200ml 400kD, 1,0mm, 0.6mm, 0.5mm 500kD*, Research II System 25 - 145 cm2 0.05µm*, Dry, Irradiated, Pre-wetted 0.1µm, 0.2µm, Disposable 0.5µm More information MiniKros Sampler R&D 10kD, PS, PES, ME 1/4" HB 50kD, FLL 100kD, 50ml - 3L 400kD, 1,0mm, 0.6mm, 0.5mm 500kD*, Research II System 95 - 615 cm2 0.05µm*, Dry, Irradiated, Pre-wetted 0.1µm, 0.2µm, Disposable 0.5µm More information MiniKros Sampler Plus " " " 1,5" TC, 3/4 TC, 3/8" HB 3/8" HB Research II System MiniKros Laboratory 10kD, PS, PES, ME 1/2" HB 50kD, FLL 100kD, 300ml - 100L 400kD, 0.6mm, 0.5mm 500kD*, Research II System, MiniKros Plus 165 – 6 600 cm2 0.05µm*, Dry, Irradiated, Pre-wetted System, MiniKros Pilot System 0.1µm, More information 0.2µm, Disposable 0.5µm MiniKros Plus 600 - 9900 cm2 " " 1,5" TC, ¾" TC, 1/2" HB, 3/8" HB 3/8" HB Research II System, MiniKros Plus System, MiniKros Pilot System KrosFlo Pilot 10kD, PS, PES, ME 3" TC 50kD, 1.5" TC 100kD, 3L – 3 000L 400kD, 0.6mm, 0.5mm 500kD*, MiniKros Pilot System, KrosFlo Pilot 0.32 - 5,1 m2 0.05µm*, Dry, Irradiated, Pre-wetted System, KrosFlo Pharma System, 0.1µm, Production Skid 0.2µm, Disposable 0.5µm Operating manual KrosFlo Plus " " " 1,5" TC, 1/2" HB, 1/4" HB 1,5" TC, 1/2 STR, 3/8" HB KrosFlo Max Production 10kD, PS, PES, ME 2" TC 50kD, 1,5" TC 100kD, 1 000L-50 000L 400kD, 0.6mm, 0.5mm 500kD*, KrosFlo® Pharma System (50-5 000L) 6.3 - 22 m2 0.05µm*, Dry, Irradiated, Pre-wetted & custom designed skids (>50 000L) 0.1µm, 0.2µm, 0.5µm More information KrosFlo Steam In Place Production 10kD, PS, PES N/A 50kD, 100kD, 1 000L-50 000L 400kD, 1,0mm, 0.5mm KrosFlo Pharma System, Production 500kD*, Skid 0.98 - 9,2 m2 0.05µm*, Dry 0.2µm, 0.5µm Disposable & More information Re-usable CellFlo Laboratory & 500kD*, PS, PES, ME 3" TC, 1/2" HB, 1/4" HB Pilot 0.2µm, 1,5" TC, FLL 300ml – 3 000L 0.5µm PS, PES, ME 500cm2 – 4.1m2 MiniKros Plus System, MiniKros Dry, Irradiated, Pre-wetted Pilot System, KrosFlo Pilot System, Disposable KrosFlo Pharma System, Production More information Skid CellFlo Plus Labscale: same same 1,5" TC, ¾" TC, 1/2" HB, 3/8" HB, 500ml-100L 1/4" HB 2 500cm2 - 6,2m 1,5" TC, ½" STR, 3/8" HB Pilotscale: 3L-1 000L 0.31m2 - 4,1m2 Same above (but MiniKros Pilot ) CellFlo MAX 50L-50 000L More information KrosFlo® Pharma System (50-5 000L) & + & custom designed skids (>50 000L) *500kD (20nm), 0.05µm (50nm) Reference nomenclature See KrosFlo modules list[] Basics of Filtration/UltraFiltration Filtration is a technique that utilizes a porous barrier to separate suspended or dissolved materials in solution based on size or molecular weight, eliminating the need for centrifugation, solvent phase changes or other product damaging methods. Application of positive or negative pressure differential across the selectively permeable barrier drives the separation in a sieve-like manner. Smaller constituents pass through the barrier with the solvent as filtrate while the larger solutes are retained. The type of filtration is based on the retentate and pore size. Macrofiltration & Microfiltration Being the simplest and least expensive, macrofiltration is conventionally defined as the filtration of particles that are 1-5 µm or greater > KrosFlow Hollow Fiber Modules allow 0.1 to 5µm filtrations for clarification of cell cultures or lysates, virus or particules suspensions. See also Spectra Mesh® screens (SS, Nylon, PS) Originally described as the separation of particles or microbes that could be seen with the aid of a microscope (cells, macrophage, large virus particles, cellular debris), microfiltration (MF) pertains to separations with the membrane pore size ranging from 0.05 to 0.5 µm in diameter. > KrosFlow Hollow Fiber Modules allow microfiltration from 20 nm to 0.5 µm and surface areas ranging from 6 sq. cm to 13.9 sq. m. See also Dynaguard (hollow fiber containing tips for pipette) and MediaKap filters (hollow fiber filters for cell culture media). Ultrafiltration & Dialysis Ultrafiltration is defined to molecular level filtration, hence with MW below0.1µm (100nm) that correspond to 1-10 millions of Dalton (1 000-10 000kDa) . Since the "molecular shape" of dissolved solutes ranging from 1 to 1 000 kD significantly affects the retention characteristics, the pore rating for ultrafiltration (UF) membranes is indirectly determined as the nominal molecular weight cut off (MWCO) that is retained by approximately 95%. An ultrafiltration is typicall yperforme by applied a pressure to allow the solution to cross the filtration membrane. An ultrafiltration process that is "passive", driven (by the newtonian motion of molecules), is generally refered as dialysis, and is operated for the lower molecular range, e.g. < 500 kDa. > KrosFlow Hollow Fiber Modules allow ultrafiltration with low protein binding polysulfone hollow fiber membranes with 10 kD, 50 kD, 400 kD, 500 kD (20 nm) and 1 000 kD (eq.0.05 µm) and surface areas ranging from 8 sq. cm to 5.1 sq. m. Cellulose Ester (CE/ME) membrane fibers are also available. The Fiber with the Competitive Edge! Spectrum combines the advantages of a hollow fiber membrane geometry with the greater efficiency of Tangential Flow Filtration to offer the best disposable microfiltration and ultrafiltration products on the market. Much like the human circulatory system, our Hollow Fiber Tangential Flow Filtration (HF TFF) membranes and systems quickly and efficiently separate R & D to production scale volumes of valuable product with higher yields. Only Spectrum provides all this at such low pricing that disposability is not only possible, it's preferable. Tangential Flow vs. Dead-End Filtration ... for less Fouling Dead-end filtration results in a build up of product on the membrane surface that may damage product, lower recovery and "foul" the membrane. Fouling impedes the filtration rate until it eventually stops. Tangential Flow Filtration (TFF) involves the recirculation of the retentate across the surface of the membrane. This gentle "cross flow" feed acts to minimize membrane fouling, maintains a high filtration rate and provides higher product recovery since the sample remains safely in solution. Spectrum has taken microfiltration and ultrafiltration to a much more efficient level by offering a full line of disposable hollow fiber tangential flow filtration (TFF) membranes. Hollow Fiber vs. Flat Sheet ... for higher yield, direct scalability & greater SA Whether stacked or spiral wound, flat sheet membranes require the product stream to spread across the entire surface of the individual sheets prior to recirculation. This non-uniform flow path causes build up and loss of product in "membrane corners" that see slower flow rates. Consequently flat sheet TFF is not directly scalable. Hollow Fiber (HF) membranes offer the efficiency of uniform flow through the lumen without product loss and build up because there are no "corners".
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