The Smell Test
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Synthesis of Low Molecular Weight Flavor Esters Using Plant Seedling Lipases in Organic Media M
JFS: Food Chemistry and Toxicology Synthesis of Low Molecular Weight Flavor Esters Using Plant Seedling Lipases in Organic Media M. LIAQUAT AND R.K.OWUSU APENTEN ABSTRACT: Powders from germinated seedlings of wheat, barley, rapeseed, maize, and linola synthesized low molecular weight flavor esters in an organic medium (hexane). Direct esterification of acetic, butyric, and caproic acids, with ethanol, butanol, isopentanol, or (Z)-3- hexen-l-ol was achieved. Of the systems examined, germinated rapeseed showed the highest degree of flavor synthesis. (Z)-3-hexen-1-yl butyrate and (Z)-3-hexen-1-yl caproate were produced with yields of about 96%. Butyl butyrate, isopentyl butyrate, butyl caproate and isopentyl caproate were produced at 80% yield. Linola seedling powder gave yields of Յ63% for ethyl acetate and butyl acetate. More moderate (40%) yields were obtained with barley and maize seedling powders. Rapeseed seedling powder is a convenient and inexpensive catalyst for preparing low molecular weight esters in organic media. Key Words: plant lipases, seedling, flavor, synthesis, organic phase biocatalysis Introduction There appear to be no reports describing the use of plant-de- OW MOLECULAR WEIGHT ESTERS (LMWE) ARE COMMON FLA- rived lipases or acetone powders for LMWE synthesis. Seed li- Lvoring agents for fruit-based products and dairy products pase or acetone powders from castor bean, rape, and Nigella sati- (Schultz and others 1967). Flavor losses during food manufactur- va seeds were used for lipid hydrolysis, glycerolysis, and esterifi- ing processes must be compensated for by additions. Production cation of glycerols or oleic acids (Hassanien and Mukherjee 1986; of LMWE is of commercial interest. -
(CA) Stored 'Golden Delicious' Apples to the Treatments with Alcohols and Aldehydes As
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/287512682 Response of controlled atmosphere (CA) stored 'Golden Delicious' apples to the treatments with alcohols and aldehydes as... Article · July 2000 CITATIONS READS 13 8 3 authors, including: Jamil Harb Birzeit University 40 PUBLICATIONS 151 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Organic Farming in Palestine View project All content following this page was uploaded by Jamil Harb on 30 December 2015. The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately. Gartenbauwissenschaft, 65 (4). S. 154–161, 2000, ISSN 0016–478X. © Verlag Eugen Ulmer GmbH & Co., Stuttgart Response of Controlled Atmosphere (CA) stored “Golden Delicious” Apples to the Treatments with Alcohols and Aldehydes as Aroma Precursors Reaktion von CA-gelagerten „Golden Delicious” Äpfeln auf die Behandlung mit Alkoholen und Aldehyden als Aromavorstufen J. Harb, J. Streif and F. Bangerth (Institut für Obst-, Gemüse- und Weinbau, Universität Hohenheim, Stuttgart, Germany) Summary Aromastoffe. Sowohl nach ULO-Lagerung wie auch „Golden Delicious” apples were stored under ultra low nach AVG-Behandlung war durch die Behandlung mit oxygen (ULO-storage) and treated both at harvest Aromavorstufen nur eine vorübergehende Wirkung, time and after 5 months of storage with several aroma im allgemeinen von nicht mehr als 2 Tagen, zu beob- precursors. Another plot of fruits was sprayed on the achten. tree with an ethylene inhibitor (AVG) to study the ef- fect of ethylene biosynthesis on volatile production. -
Expanding the Modular Ester Fermentative Pathways for Combinatorial Biosynthesis of Esters from Volatile Organic Acids
ARTICLE Expanding the Modular Ester Fermentative Pathways for Combinatorial Biosynthesis of Esters From Volatile Organic Acids Donovan S. Layton,1,2 Cong T. Trinh1,2,3 1 Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 2 BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 3 Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, Tennessee; telephone: þ865-974-8121; fax: 865-974-7076; e-mail: [email protected] Biotechnol. Bioeng. 2016;113: 1764–1776. ABSTRACT: Volatile organic acids are byproducts of fermentative ß 2016 Wiley Periodicals, Inc. metabolism, for example, anaerobic digestion of lignocellulosic KEYWORDS: modular chassis cell; carboxylate; ester; acyl acetate; biomass or organic wastes, and are often times undesired inhibiting acyl acylate; ester fermentative pathway cell growth and reducing directed formation of the desired products. Here, we devised a general framework for upgrading these volatile organic acids to high-value esters that can be used as flavors, fragrances, solvents, and biofuels. This framework employs the acid-to-ester modules, consisting of an AAT (alcohol Introduction acyltransferase) plus ACT (acyl CoA transferase) submodule and an alcohol submodule, for co-fermentation of sugars and organic Harnessing renewable or waste feedstocks (e.g., switchgrass, corn acids to acyl CoAs and alcohols to form a combinatorial library of stover, agricultural residue, or municipal solid waste) -
Download Author Version (PDF)
Analytical Methods Accepted Manuscript This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication. Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available. You can find more information about Accepted Manuscripts in the Information for Authors. Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content. The journal’s standard Terms & Conditions and the Ethical guidelines still apply. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains. www.rsc.org/methods Page 1 of 23 Analytical Methods 1 2 3 1 Analysis of volatile compounds in Capsicum spp. by headspace solid-phase 4 5 6 2 microextraction and GC×GC-TOFMS 7 8 3 Stanislau Bogusz Junior a, d *, Paulo Henrique Março b, Patrícia Valderrama b, Flaviana 9 10 c c c 11 4 Cardoso Damasceno , Maria Silvana Aranda , Cláudia Alcaraz Zini , Arlete Marchi 12 d e 13 5 Tavares Melo , Helena Teixeira Godoy 14 15 6 16 17 18 7 a Federal University of the Jequitinhonha and Mucuri (UFVJM), Institute of Science and 19 20 8 Technology, Diamantina, MG, Brazil. -
Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida Rugosa Lipase Immobilized in Microemulsion Based Organogels: Effect of Parameters and Reusability
Hindawi Publishing Corporation BioMed Research International Volume 2014, Article ID 353845, 14 pages http://dx.doi.org/10.1155/2014/353845 Research Article Biocatalytic Synthesis of Flavor Ester ‘‘Pentyl Valerate’’ Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels: Effect of Parameters and Reusability Tripti Raghavendra,1 Nilam Panchal,1 Jyoti Divecha,2 Amita Shah,1 and Datta Madamwar1 1 BRD School of Biosciences, Sardar Patel Maidan, Sardar Patel University, Satellite Campus, Vadtal Road, P.O. Box 39, Vallabh Vidyanagar, Gujarat 388120, India 2 Department of Statistics, Sardar Patel University, Vallabh Vidyanagar, Gujarat 388120, India Correspondence should be addressed to Datta Madamwar; datta [email protected] Received 28 February 2014; Revised 5 May 2014; Accepted 19 May 2014; Published 1 July 2014 Academic Editor: Yunjun Yan Copyright © 2014 Tripti Raghavendra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Pentyl valerate was synthesized biocatalytically using Candida rugosa lipase (CRL) immobilized in microemulsion based organogels ∘ (MBGs). The optimum conditions were found to be pH 7.0, temperature of 37 C, ratio of concentration of water to surfactant (Wo) of 60, and the surfactant sodium bis-2-(ethylhexyl)sulfosuccinate (AOT) for MBG preparation. Although kinetic studies revealed that the enzyme in free form had high affinity towards substrates ( = 23.2 mM for pentanol and 76.92 mM for valeric acid) whereas, after immobilization, the values increased considerably (74.07 mM for pentanol and 83.3 mM for valeric acid) resulting in a slower reaction rate, the maximum conversion was much higher in case of immobilized enzyme (∼99%) as compared to free enzyme (∼19%). -
Articles Is an Important Step for the For- Mation and Transformation of Atmospheric Particles
Atmos. Chem. Phys., 15, 5585–5598, 2015 www.atmos-chem-phys.net/15/5585/2015/ doi:10.5194/acp-15-5585-2015 © Author(s) 2015. CC Attribution 3.0 License. Compilation and evaluation of gas phase diffusion coefficients of reactive trace gases in the atmosphere: Volume 2. Diffusivities of organic compounds, pressure-normalised mean free paths, and average Knudsen numbers for gas uptake calculations M. J. Tang1, M. Shiraiwa2, U. Pöschl2, R. A. Cox1, and M. Kalberer1 1Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK 2Multiphase Chemistry Department, Max Planck Institute for Chemistry, 55128, Mainz, Germany Correspondence to: M. J. Tang ([email protected]) and M. Kalberer ([email protected]) Received: 26 January 2015 – Published in Atmos. Chem. Phys. Discuss.: 25 February 2015 Revised: 24 April 2015 – Accepted: 3 May 2015 – Published: 21 May 2015 Abstract. Diffusion of organic vapours to the surface of 1 Introduction aerosol or cloud particles is an important step for the for- mation and transformation of atmospheric particles. So far, Organic aerosols are ubiquitous in the atmosphere and can however, a database of gas phase diffusion coefficients for or- account for a dominant fraction of submicron aerosol par- ganic compounds of atmospheric interest has not been avail- ticles (Jimenez et al., 2009). Organic aerosols affect climate able. In this work we have compiled and evaluated gas phase by scattering and adsorbing solar and terrestrial radiation and diffusivities (pressure-independent diffusion coefficients) of serving as cloud condensation nuclei and ice nuclei (Kanaki- organic compounds reported by previous experimental stud- dou et al., 2005; Hallquist et al., 2009). -
Microbial Synthesis of a Branched-Chain Ester Platform from Organic Waste Carboxylates
Metabolic Engineering Communications 3 (2016) 245–251 Contents lists available at ScienceDirect Metabolic Engineering Communications journal homepage: www.elsevier.com/locate/mec Microbial synthesis of a branched-chain ester platform from organic waste carboxylates Donovan S. Layton a,c, Cong T. Trinh a,b,c,n a Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, The United States of America b Bredesen Center for Interdisciplinary Research and Graduate Education, The University of Tennessee, Knoxville, The United States of America c Bioenergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, The United States of America article info abstract Article history: Processing of lignocellulosic biomass or organic wastes produces a plethora of chemicals such as short, Received 6 June 2016 linear carboxylic acids, known as carboxylates, derived from anaerobic digestion. While these carbox- Received in revised form ylates have low values and are inhibitory to microbes during fermentation, they can be biologically 15 July 2016 upgraded to high-value products. In this study, we expanded our general framework for biological up- Accepted 5 August 2016 grading of carboxylates to branched-chain esters by using three highly active alcohol acyltransferases Available online 6 August 2016 (AATs) for alcohol and acyl CoA condensation and modulating the alcohol moiety from ethanol to iso- Keywords: butanol in the modular chassis cell. With this framework, we demonstrated the production of an ester Carboxylate platform library comprised of 16 out of all 18 potential esters, including acetate, propionate, butanoate, pen- Ester platform tanoate, and hexanoate esters, from the 5 linear, saturated C -C carboxylic acids. -
N-AMYL BUTYRATE (Code: NAB)
Balsamic Floral Fruity Green Minty Phenolic Powdery Spicy Woody n-AMYL BUTYRATE (Code: NAB) Olfactive Note: Ethereal-fruity, Banana, Pineapple, Tropical odor Extensively used in flavor compositions for imitation Apple, Apricot, Banana, Butter, Butterscotch, Cherry, Fruit Grape, Peach, Pineapple, Raspberry, Strawberry, Vanilla, etc. Chemical Formula C9H18O2 Up to 760 ppm in Molecular Weight (gm/Mol) 158.24 Flavor Use Log P (o/w) 3.320 Level Up to 8% in Solubility in Water @ 25 0C 60 mg/L pentyl butanoate Fragrance ✔ Synthetic substance Nature-Identical Artificial ✔ Food Grade Kosher PHYSICO-CHEMICAL PROPERTIES REGULATORY REFERENCES Appearance Clear colorless liquid CAS No. 540-18-1 Purity (by GLC) 98% min. FEMA 2059 Specific Gravity 0.863 - 0.866 @ 25 0C EINECS 208-739-2 Refractive Index 1.4090 - 1.4140 @ 20 0C CoE 270 Boiling Point 185 0C to 187 0C @ 760 mmHg FL No. 09.044 Flash Point (TCC) 67.78 0C JECFA 152 Tenacity 4 Hrs at 100% FDA Regulation 21 CFR 172.515 Solubility in Ethanol 1ml soluble in 1ml 95% Alcohol Food Chemical Codex Listed Acid Value 1 max. (mgKOH/gm) REACH Pre-Reg. No. --- Export Tariff Code 2915.60.5000 Vapour Pressure 0.569000 mmHg @ 25 0C Vapour Density 5.4 (Air=1) Anti-Oxidants/Stabilizers Yes ✔ No Heat of Vaporization (ΔvapH°) 44.78 kJ/mol Derived from GMO? Yes GMO as process aid? Synonyms: pentyl butyrate; Butyric acid, pentyl ester; 1-pentyl butyrate; n-pentyl butyrate; Amyl butanoate; N-amyl butanoate; amyl butyrate. Packing: As per Customer's requirement Shelf life of 24 months from the date of manufacturing. -
Third Supplement, FCC 11 Index / All-Trans-Lycopene / I-1
Third Supplement, FCC 11 Index / All-trans-Lycopene / I-1 Index Titles of monographs are shown in the boldface type. A 2-Acetylpyridine, 20 Alcohol, 80%, 1524 3-Acetylpyridine, 21 Alcohol, 90%, 1524 Abbreviations, 6, 1726, 1776, 1826 2-Acetylpyrrole, 21 Alcohol, Absolute, 1524 Absolute Alcohol (Reagent), 5, 1725, 2-Acetyl Thiazole, 18 Alcohol, Aldehyde-Free, 1524 1775, 1825 Acetyl Valeryl, 562 Alcohol C-6, 579 Acacia, 556 Acetyl Value, 1400 Alcohol C-8, 863 ªAccuracyº, Defined, 1538 Achilleic Acid, 24 Alcohol C-9, 854 Acesulfame K, 9 Acid (Reagent), 5, 1725, 1775, 1825 Alcohol C-10, 362 Acesulfame Potassium, 9 Acid-Hydrolyzed Milk Protein, 22 Alcohol C-11, 1231 Acetal, 10 Acid-Hydrolyzed Proteins, 22 Alcohol C-12, 681 Acetaldehyde, 10 Acid Calcium Phosphate, 219, 1838 Alcohol C-16, 569 Acetaldehyde Diethyl Acetal, 10 Acid Hydrolysates of Proteins, 22 Alcohol Content of Ethyl Oxyhydrate Acetaldehyde Test Paper, 1535 Acidic Sodium Aluminum Phosphate, Flavor Chemicals (Other than Acetals (Essential Oils and Flavors), 1065 Essential Oils), 1437 1395 Acidified Sodium Chlorite Alcohol, Diluted, 1524 Acetanisole, 11 Solutions, 23 Alcoholic Potassium Hydroxide TS, Acetate C-10, 361 Acidity Determination by Iodometric 1524 Acetate Identification Test, 1321 Method, 1437 Alcoholometric Table, 1644 Aceteugenol, 464 Acid Magnesium Phosphate, 730 Aldehyde C-6, 571 Acetic Acid Furfurylester, 504 Acid Number (Rosins and Related Aldehyde C-7, 561 Acetic Acid, Glacial, 12 Substances), 1418 Aldehyde C-8, 857 Acetic Acid TS, Diluted, 1524 Acid Phosphatase -
ENGLISH POSITIVE LIST MATERIAL (ENG).Pdf
Attachment of Decree Number: SK15/Dir/LPPOM MUI/XI/19 HALAL POSITIVE LIST OF MATERIALS Halal Positive List of Materials consists of non critical materials, in terms of their halalness status, commonly used in prosessing industries. This material list was made based on the assessment of LPPOM MUI refer to the literature, abundance in nature, and consideration of commercially production scales. Companies that using materials listed in Halal Positive List of Materials will get advantages as follows: 1. In selection process of new materials, materials listed in Halal Positive List of Materials already get material approval from LPPOM MUI automatically prior to use. 2. In process of incoming material checking, materials listed in Halal Positive List of Materials does not require the suitability checking of the material name, manufacture's name and country of origin. 3. In product registration process, materials listed in Halal Positive List of Materials does not require supporting document. If the materials use trade name which is different with material name, the material specification document is still required. During the audit process, the auditor may check the supporting documents of material when necessary. Halal Positive List of Materials is given in the following table: Prohibited for Foods (BPOM CAS Chemical Name Regulation) Foods Cosmetics 673-84-7 2,6-Dimethyl-2,4,6-octatriene 91-57-6 2-Methylnaphthalene 1576-78-9 [(E)-hept-3-enyl] acetate 72214-23-4 7-acetoxy-3,7-dimethyl-octa-1,3- diene Page 3 of 192 2216-45-7 (4-methylphenyl)methyl -
Door 2.0 - Comprehensive Mapping of Drosophila Melanogaster Odorant Responses
DoOR 2.0 - Comprehensive Mapping of Drosophila melanogaster Odorant Responses Daniel Münch1,* and C. Giovanni Galizia1 1Neurobiology, University of Konstanz, 78457 Konstanz, Germany *[email protected] Supplementary Figures & Tables Or47b overlap 5 Or47b overlap 3 Or47b Dweck LTK: 0.25; n: 178 LTK: 3.4; n: 218 LTK: 33.12; n: 43 0.5 0.5 0.5 0.0 0.0 0.0 DoOR response −0.5 −0.5 −0.5 odorants ab4B all ab4B geosmin LTK: −1.53; n: 182 LTK: 89.35; n: 181 0.5 0.5 0.0 0.0 DoOR response −0.5 −0.5 odorants (a) model: inv.sigmoid model: inv.asympOff model: sigmoid n = 12 n = 10 n = 16 MD = 0.093643 MD = 0.140333 MD = 0.052509 merged_data merged_data Hallem.2004.EN 0.0 0.4 0.8 0.0 0.4 0.8 0.0 0.4 0.8 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.4 0.8 Hallem.2006.EN Pelz.2005.Or47bnmr Hallem.2004.WT model: inv.linear model: inv.linear n = 44 n = 3 MD = 0.124972 MD = 0.027766 merged_data merged_data 0.0 0.5 1.0 0.0 0.5 1.0 1.5 0.0 0.2 0.4 0.6 0.8 1.0 0.0 0.4 0.8 Muench.2015.AntGC1 Dweck.2015b.WT (b) Figure S1: Related to Figure3. Merging two narrowly tuned responding units with different merge-specifications. -
X International Conference “Mechanisms of Catalytic Reactions”
Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia Lomonosov Moscow State University, Moscow, Russia 2016 X International Conference “Mechanisms of Catalytic Reactions” Svetlogorsk, Kaliningrad Region, Russia October 2 - 6, 2016 ABSTRACTS Novosibirsk-2016 Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia Lomonosov Moscow State University, Moscow, Russia X International Conference “Mechanisms of Catalytic Reactions” Svetlogorsk, Kaliningrad Region, Russia October 2 - 6, 2016 ABSTRACTS Novosibirsk-2016 УДК 544.47+66.09 ББК Г544 M45 Mechanisms of Catalytic Reactions. X International Conference (MCR-X). (October 2 - 6, 2016, Svetlogorsk, Kaliningrad Region, Russia) [Electronic resourse]: Book of abstracts / Boreskov Institute of Catalysis SB RAS ed.: prof. V.I. Bukhtiyarov, - Novosibirsk: BIC, 2016. p.328, – 1 electronic optical disc (CD-R). ISBN 978-5-906376-15-2 В надзаг.: Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia Zelinsky Institute of Organic Chemistry RAS, Moscow, Russia Lomonosov Moscow State University, Moscow, Russia Topics of book: – First-principles approach, theory and simulation in catalysis; – Advanced methods for studies of mechanisms of catalyzed reactions; – In-situ and operando studies of model and real catalysts; – Kinetics and reaction intermediates of catalyzed processes; – From mechanistic studies to design of advanced catalyst systems. The Conference is accompanied