Adipocytes As a New Source of Catecholamine Production
CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - Publisher Connector FEBS Letters 585 (2011) 2279–2284 journal homepage: www.FEBSLetters.org Adipocytes as a new source of catecholamine production ⇑ Peter Vargovic a, , Jozef Ukropec b, Marcela Laukova a, Susannah Cleary d, Bernhard Manz c, Karel Pacak d, Richard Kvetnansky a a Laboratory of Stress Research, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 83306 Bratislava, Slovakia b Diabetes and Metabolic Research Laboratory, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 83306 Bratislava, Slovakia c LDN Labor Diagnostika Nord, D-48531 Nordhorn, Germany d Section on Medical Neuroendocrinology, NICHD, NIH, Bethesda, MD, USA article info abstract Article history: Catecholamines are an important regulator of lipolysis in adipose tissue. Here we show that rat adi- Received 2 February 2011 pocytes, isolated from mesenteric adipose tissue, express genes of catecholamine biosynthetic Revised 28 April 2011 enzymes and produce catecholamines de novo. Administration of tyrosine hydroxylase inhibitor, Accepted 1 June 2011 alpha-methyl-p-tyrosine, in vitro significantly reduced concentration of catecholamines in isolated Available online 16 June 2011 adipocytes. We hypothesize that the sympathetic innervation of adipose tissues is not the only Edited by Laszlo Nagy source of catecholamines, since adipocytes also have the capacity to produce both norepinephrine and epinephrine. Ó 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Keywords: Adipocyte Endogenous catecholamine Catecholamine biosynthetic enzyme Gene expression 1. Introduction catecholamine biosynthesis. PNMT mRNA and immunoreactive protein were found in the adipose tissue in neuron-like cells of Adipose tissue is a specialized organ serving several functions PNMT-overexpressing transgenic mice but not in wild types [10].
[Show full text]