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Amphibolic Nature of Krebs Cycle
Amphibolic nature of Krebs Cycle How what we are is what we eat • In aerobic organisms, the citric acid cycle is an amphibolic pathway, one that serves in both catabolic and anabolic processes. • Since the citric acid does both synthesis (anabolic) and breakdown (catabolic) activities, it is called an amphibolic pathway • The citric acid cycle is amphibolic (i.e it is both anabolic and catabolic in its function). • It is said to be an AMPHIBOLIC pathway, because it functions in both degradative or catabolic and biosynthetic or anabolic reactions (amphi = both) A central metabolic pathway or amphibolic pathway is a set of reactions which permit the interconversion of several metabolites, and represents the end of the catabolism and the beginning of anabolism • The KREBS CYCLE or citric acid cycle is a series of reactions that degrades acetyl CoA to yield carbon dioxide, and energy, which is used to produce NADH, H+ and FADH. • The KREBS CYCLE connects the catabolic pathways that begin with the digestion and degradation of foods in stages 1 and 2 with the oxidation of substrates in stage 3 that generates most of the energy for ATP synthesis. • The citric acid cycle is the final common pathway in the oxidation of fuel molecules. In stage 3 of metabolism, citric acid is a final common catabolic intermediate in the form of acetylCoA. • This is why the citric acid cycle is called a central metabolic pathway. Anaplerosis and Cataplerosis Anaplerosis is a series of enzymatic reactions in which metabolic intermediates enter the citric acid cycle from the cytosol. Cataplerosis is the opposite, a process where intermediates leave the citric acid cycle and enter the cytosol. -
The Citric Acid Cycle the Catabolism of Acetyl-Coa
Al-Sham Private University Faculty Of Pharmacy The Citric Acid Cycle The Catabolism of Acetyl-CoA Lecturer Prof. Abboud Al-Saleh 1 Prof.Abboud AL-Saleh 10/1/2018 BIOMEDICAL IMPORTANCE • The citric acid cycle (Krebs cycle, tricarboxylic acid cycle) is a series of reactions in mitochondria that oxidize acetyl residues (as acetyl-CoA) and reduce coenzymes that upon reoxidation are linked to the formation of ATP. • TCA is the final common pathway for the aerobic oxidation of carbohydrate, lipid, and protein because glucose, fatty acids, and most amino acids are metabolized to acetyl-CoA or intermediates of the cycle. 2 Prof.Abboud AL-Saleh 10/1/2018 • TCA also has a central role in gluconeogenesis, lipogenesis, and interconversion of amino acids. Many of these processes occur in most tissues, but the liver is the only tissue in which all occur to a significant extent. • The repercussions are therefore profound when, for example, large numbers of hepatic cells are damaged as in acute hepatitis or as in cirrhosis. • Very few, if any, genetic abnormalities of TCA enzymes have been reported; such abnormalities would be incompatible with life or normal development 3 Prof.Abboud AL-Saleh 10/1/2018 Summary • The cycle starts with reaction between the acetyl moiety of acetyl-CoA and the four-carbon dicarboxylic acid oxaloacetate, forming a six-carbon tricarboxylic acid, citrate. • In the subsequent reactions, two molecules of CO2 are released and oxaloacetate is regenerated (Figure). • Only a small quantity of oxaloacetate is needed for the oxidation of a large quantity of acetyl-CoA. • oxaloacetate may be considered to play a catalytic role. -
The Electron Transport Chain in Anaerobically Functioning Eukaryotes
rl BIOCHIMICA ET BIOPHYSICA ACTA II BBt ELSEVIER Biochimica et Biophysica Acta, 1365 (1998) 71-78 The electron transport chain in anaerobically functioning eukaryotes Aloysius G.M. Tielens*, Jaap J. Van Hellemond Laboratory of Veterinary Biochemistry and Institute for Biomembranes, Utrecht University, P.O. Box 80176, 3508 TD Utrecht, The Netherlands Received 27 January 1998; received in revised form 26 February 1998; accepted 2 March 1998 Abstract Many lower eukaryotes can survive anaerobic conditions via a fermentation pathway that involves the use of the reduction of endogenously produced fumarate as electron sink. This fumarate reduction is linked to electron transport in an especially adapted, anaerobically functioning electron-transport chain. An aerobic energy metabolism with Krebs cycle activity is accompanied by electron transfer from succinate to ubiquinone via complex II of the respiratory chain. On the other hand, in an anaerobic metabolism, where fumarate functions as terminal electron acceptor, electrons are transferred from rhodoquinone to fumarate, which is the reversed direction. Ubiquinone cannot replace rhodoquinone in the process of fumarate reduction in vivo, as ubiquinone can only accept electrons from complex II and cannot donate them to fumarate. Rhodoquinone, with its lower redox potential than ubiquinone, is capable of donating electrons to fumarate. Eukaryotic fumarate reductases were shown to interact with rhodoquinone (a benzoquinone), whereas most prokaryotic fumarate reductases interact with the naphtoquinones mena- quinone and demethylmenaquinone. Fumarate reductase, the enzyme essential for the anaerobic functioning of many eukaryotes, is structurally very similar to succinate dehydrogenase, the Krebs cycle enzyme catalysing the reverse reaction. In prokaryotes these enzymes are differentially expressed depending on the external conditions. -
Catabolism Iii
Nitrogen Catabolism Glycogenolysis Protein Fat catabolism Catabolism Fatty Acid Amino-acid GlycolysisDegradation catabolism Pyruvate Oxidation Krebs' Cycle Phosphorylation Oxidative CATABOLISM III: Digestion and Utilization of Proteins • Protein degradation • Protein turnover – The ubiquitin pathway – Protein turnover is tightly regulated • Elimination of nitrogen – By fish, flesh and fowl – How is the N of amino acids liberated and eliminated? • How are amino acids oxidized for energy 1 Protein Catabolism Sources of AMINO ACIDS: •Dietary amino acids that exceed body’s protein synthesis needs •Excess amino acids from protein turnover (e.g., proteolysis and regeneration of proteins) •Proteins in the body can be broken down (muscle wasting) to supply amino acids for energy when carbohydrates are scarce (starvation, diabetes mellitus). •Carnivores use amino acids for energy more than herbivores, plants, and most microorganisms Protein Catabolism The Digestion Pathway • Pro-enzymes are secreted (zymogens) and the environment activates them by specific proteolysis. • Pepsin hydrolyzes protein into peptides in the stomach. • Trypsin and chymotrypsin hydrolyze proteins and larger peptides into smaller peptides in the small intestine. • Aminopeptidase and carboxypeptidases A and B degrade peptides into amino acids in the small intestine. 2 Protein Catabolism The Lysosomal Pathway • Endocytosis, either receptor-mediated, phagocytosis, or pinocytosis engulfs extra- cellular proteins into vesicles. • These internal vesicles fuse as an early endosome. • This early endosome is acidified by the KFERQ Substrates vATPase (“v” for vesicular). • Components that are recycled, like receptors, HSPA8 are sequestered in smaller vesicles to create Co-chaperones the multivesicular body (MVB), sometimes called a late endosome. • If set for degradation, it will fuse with a KFERQ primary lysosome (red) which contains many cathepsin-type proteases. -
Uncoupling Proteins: Functional Characteristics and Role in the Pathogenesis of Obesity and Type II Diabetes
Diabetologia 2001) 44: 946±965 Ó Springer-Verlag 2001 Uncoupling proteins: functional characteristics and role in the pathogenesis of obesity and Type II diabetes L. T.Dalgaard, O.Pedersen Steno Diabetes Center, Gentofte, Denmark Abstract obesity. The three uncoupling protein homologue genes UCP1, UCP2, and UCP3 have been investigat- Uncoupling proteins are mitochondrial carrier pro- ed for polymorphisms and mutations and their impact teins which are able to dissipate the proton gradient on Type II diabetes mellitus, obesity, and body weight of the inner mitochondrial membrane. This uncou- gain or BMI. The main conclusion is that variation in pling process reduces the amount of ATP generated the UCP1, UCP2 or UCP3 genes is not associated through an oxidation of fuels. The hypothesis that un- with major alterations of body weight gain. The con- coupling proteins UCPs) are candidate genes for hu- tribution of UCP genes towards polygenic obesity man obesity or Type II non-insulin-dependent) dia- and Type II diabetes is evaluated and discussed. [Dia- betes mellitus is based on the finding that a chemical betologia 2001) 44: 946±965] uncoupling of the mitochondrial membrane reduces body adiposity, and that lower metabolic rates predict Keywords Uncoupling proteins, Type II diabetes weight gain. It is straightforward to hypothesize that mellitus, obesity, genetics, body weight regulation, common polymorphisms of UCP1, UCP2 and UCP3 energy expenditure, metabolic rate, brown adipose genes lower metabolic rate by a more efficient energy tissue, white adipose tissue, reactive oxygen species, coupling in the mitochondria. Furthermore, geneti- polymorphism, mutation, transgenics, gene knock- cally engineered mice over expressing different UCP out. -
Biol 1020: Photosynthesis
Chapter 10: Photosynthesis Energy and Carbon Sources Electromagnetic Spectrum and Light Chloroplasts Photosynthesis Overview Light Reactions C3 Cycle Photorespiration Supplemental Carbon Fixation: C4 and CAM pathways . • List and differentiate the 4 possible groups of organisms based on how they obtain energy and useful carbon. Classification by Energy and Carbon Sources energy source chemotrophs can only get energy directly from chemical compounds phototrophs can get energy directly from light (these organisms can use chemical compounds as energy sources as well) . Classification by Energy and Carbon Sources carbon source autotrophs can fix carbon dioxide, thus they can use CO2 as a carbon source heterotrophs cannot fix CO2; they use organic molecules from other organisms as a carbon source . Classification by Energy and Carbon Sources combined, these leads to 4 possible groups: photoautotrophs – carry out photosynthesis use light energy to fix CO2 store energy in chemical bonds of organic molecules includes green plants, algae, and some bacteria photoheterotrophs – use light energy but cannot fix CO2; some nonsulfur purple bacteria chemoautotrophs – obtain energy from reduced inorganic molecules and use some of it to fix CO2; some bacteria chemoheterotrophs – use organic molecules as both carbon and energy sources dependent completely on other organisms for energy capture and carbon fixation includes all animals, all fungi, most protists, and most bacteria . • List and differentiate the 4 possible groups of -
Identification of Alternative Mitochondrial Electron Transport
International Journal of Molecular Sciences Article Identification of Alternative Mitochondrial Electron Transport Pathway Components in Chickpea Indicates a Differential Response to Salinity Stress between Cultivars Crystal Sweetman * , Troy K. Miller, Nicholas J. Booth, Yuri Shavrukov , Colin L.D. Jenkins, Kathleen L. Soole and David A. Day College of Science & Engineering, Flinders University, GPO Box 5100, Adelaide SA 5001, Australia; troy.miller@flinders.edu.au (T.K.M.); nick.booth@flinders.edu.au (N.J.B.); yuri.shavrukov@flinders.edu.au (Y.S.); colin.jenkins@flinders.edu.au (C.L.D.J.); kathleen.soole@flinders.edu.au (K.L.S.); david.day@flinders.edu.au (D.A.D.) * Correspondence: crystal.sweetman@flinders.edu.au; Tel.: +61-8-82012790 Received: 25 April 2020; Accepted: 27 May 2020; Published: 28 May 2020 Abstract: All plants contain an alternative electron transport pathway (AP) in their mitochondria, consisting of the alternative oxidase (AOX) and type 2 NAD(P)H dehydrogenase (ND) families, that are thought to play a role in controlling oxidative stress responses at the cellular level. These alternative electron transport components have been extensively studied in plants like Arabidopsis and stress inducible isoforms identified, but we know very little about them in the important crop plant chickpea. Here we identify AP components in chickpea (Cicer arietinum) and explore their response to stress at the transcript level. Based on sequence similarity with the functionally characterized proteins of Arabidopsis thaliana, five putative internal (matrix)-facing NAD(P)H dehydrogenases (CaNDA1-4 and CaNDC1) and four putative external (inter-membrane space)-facing NAD(P)H dehydrogenases (CaNDB1-4) were identified in chickpea. -
Fatty Acid Biosynthesis
BI/CH 422/622 ANABOLISM OUTLINE: Photosynthesis Carbon Assimilation – Calvin Cycle Carbohydrate Biosynthesis in Animals Gluconeogenesis Glycogen Synthesis Pentose-Phosphate Pathway Regulation of Carbohydrate Metabolism Anaplerotic reactions Biosynthesis of Fatty Acids and Lipids Fatty Acids contrasts Diversification of fatty acids location & transport Eicosanoids Synthesis Prostaglandins and Thromboxane acetyl-CoA carboxylase Triacylglycerides fatty acid synthase ACP priming Membrane lipids 4 steps Glycerophospholipids Control of fatty acid metabolism Sphingolipids Isoprene lipids: Cholesterol ANABOLISM II: Biosynthesis of Fatty Acids & Lipids 1 ANABOLISM II: Biosynthesis of Fatty Acids & Lipids 1. Biosynthesis of fatty acids 2. Regulation of fatty acid degradation and synthesis 3. Assembly of fatty acids into triacylglycerol and phospholipids 4. Metabolism of isoprenes a. Ketone bodies and Isoprene biosynthesis b. Isoprene polymerization i. Cholesterol ii. Steroids & other molecules iii. Regulation iv. Role of cholesterol in human disease ANABOLISM II: Biosynthesis of Fatty Acids & Lipids Lipid Fat Biosynthesis Catabolism Fatty Acid Fatty Acid Degradation Synthesis Ketone body Isoprene Utilization Biosynthesis 2 Catabolism Fatty Acid Biosynthesis Anabolism • Contrast with Sugars – Lipids have have hydro-carbons not carbo-hydrates – more reduced=more energy – Long-term storage vs short-term storage – Lipids are essential for structure in ALL organisms: membrane phospholipids • Catabolism of fatty acids –produces acetyl-CoA –produces reducing -
Nrg4 and Gpr120 Signalling in Brown Fat Anthony Chukunweike OKOLO
Nrg4 and Gpr120 Signalling in Brown Fat Anthony Chukunweike OKOLO Institute of Reproductive and Developmental Biology Department of Surgery and Cancer Faculty of Medicine Imperial College London A thesis submitted in fulfilment of the requirements for award of the degree of Doctor of Philosophy 1 Statement of Originality All experiments included in this thesis were performed by me unless otherwise stated in the text. 2 Copyright statement The copyright of this thesis rests with the author and is made available under a Creative Commons Attribution Non-Commercial No Directive Licence. Researchers are free to copy, distribute or transmit the thesis on the condition that they attribute it, and they do not use it for commercial purposes, and they do not alter, transform or build upon it. For any re-use or re-distribution, researchers must make clear to others the licence terms of this work. 3 Acknowledgments I would like to thank my supervisors Dr Aylin Hanyaloglu and Dr Mark Christian for giving me the great opportunity to work in their labs. Aylin put in a great deal of effort especially in area of Gpr120 signalling, including having to guide me through the critical imaging procedures. Aylin and Mark contributed a great deal towards the final edition of this thesis. I would also like to thank Dr Mark Christian for bringing me to Imperial College London to start off my PhD in his laboratory, and for being a great mentor and a continuous source of knowledge for me. I am grateful for your enduring patience in trying to bring out the best in me and ensuring that I develop the ‘critical thinking’ that is needed as a scientist. -
Sulphate-Reducing Bacteria's Response to Extreme Ph Environments and the Effect of Their Activities on Microbial Corrosion
applied sciences Review Sulphate-Reducing Bacteria’s Response to Extreme pH Environments and the Effect of Their Activities on Microbial Corrosion Thi Thuy Tien Tran 1 , Krishnan Kannoorpatti 1,* , Anna Padovan 2 and Suresh Thennadil 1 1 Energy and Resources Institute, College of Engineering, Information Technology and Environment, Charles Darwin University, Darwin, NT 0909, Australia; [email protected] (T.T.T.T.); [email protected] (S.T.) 2 Research Institute for the Environment and Livelihoods, College of Engineering, Information Technology and Environment, Charles Darwin University, Darwin, NT 0909, Australia; [email protected] * Correspondence: [email protected] Abstract: Sulphate-reducing bacteria (SRB) are dominant species causing corrosion of various types of materials. However, they also play a beneficial role in bioremediation due to their tolerance of extreme pH conditions. The application of sulphate-reducing bacteria (SRB) in bioremediation and control methods for microbiologically influenced corrosion (MIC) in extreme pH environments requires an understanding of the microbial activities in these conditions. Recent studies have found that in order to survive and grow in high alkaline/acidic condition, SRB have developed several strategies to combat the environmental challenges. The strategies mainly include maintaining pH homeostasis in the cytoplasm and adjusting metabolic activities leading to changes in environmental pH. The change in pH of the environment and microbial activities in such conditions can have a Citation: Tran, T.T.T.; Kannoorpatti, significant impact on the microbial corrosion of materials. These bacteria strategies to combat extreme K.; Padovan, A.; Thennadil, S. pH environments and their effect on microbial corrosion are presented and discussed. -
Coupling of Energy to Active Transport of Amino Acids in Escherichia Coli (Mutants/Membrane Vesicles/Ca,Mg-Atpase/Electron Transport/D-Lactate Dehydrogenase) ROBERT D
Proc. Nat. Acad. Sci. USA Vol. 69, No. 9, pp. 2663-2667, September 1972 Coupling of Energy to Active Transport of Amino Acids in Escherichia coli (mutants/membrane vesicles/Ca,Mg-ATPase/electron transport/D-lactate dehydrogenase) ROBERT D. SIMONI AND MARY K. SHALLENBERGER Department of Biological Sciences, Stanford University, Stanford, California 94305 Communicated by Charles Yanofsky, July 6, 1972 ABSTRACT Active transport of amino acids in isolated *to the lack of oxygen (3). Streptococcus faecalis, an anaerobe membrane vesicles of E. coli ML 308-225 is stimulated by that contains no electron-transport system, can carry out oxidation of D-lactate, and this stimulation is dependent the on electron transport [Kaback, H. R. & Milner, L. S. active membrane transport, presumably using energy- (1970) Proc. Nat. Acad. Sci. USA 66, 1008]. In attempting to yielding reactions of glycolysis (7). It has become essential to relate these results to amino-acid transport in intact relate the observations obtained with isolated vesicles to cells, we isolated mutants of E. coli ML 308-225 that physiological realities of intact cells. We have isolated cells contain defects in D-lactate dehydrogenase (EC 1.1.2.4) in various components involved in and electron transport. Intact cells of these mutants are that contain mutations normal for transport of proline and alanine. We also aerobic metabolism and have tested the effects of these muta- isolated mutants defective in Ca,Mg-stimulated ATPase tions on the ability to perform active transport of amino acids. (EC 3.6.1.3), which is responsible for coupling electron transport to the synthesis of ATP. -
Redox Potential of the Terminal Quinone Electron Acceptor QB in Photosystem II Reveals the Mechanism of Electron Transfer Regulation
Redox potential of the terminal quinone electron acceptor QB in photosystem II reveals the mechanism of electron transfer regulation Yuki Kato1, Ryo Nagao, and Takumi Noguchi1 Division of Material Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan Edited by Pierre A. Joliot, Institut de Biologie Physico-Chimique, Paris, France, and approved December 4, 2015 (received for review October 12, 2015) Photosystem II (PSII) extracts electrons from water at a Mn4CaO5 (Em values). Both forward and backward electron transfers are cluster using light energy and then transfers them to two plas- important; backward electron transfers control charge recombina- toquinones, the primary quinone electron acceptor QA and the tion in PSII, and this serves as photoprotection for PSII proteins secondary quinone electron acceptor QB. This forward electron (5, 14–17). PSII involves specific mechanisms to regulate for- transfer is an essential process in light energy conversion. Mean- ward and backward electron transfer reactions in response to while, backward electron transfer is also significant in photopro- environmental changes. For instance, in strong light, some spe- tection of PSII proteins. Modulation of the redox potential (Em) gap cies of cyanobacteria increase the Em of Pheo to facilitate charge of QA and QB mainly regulates the forward and backward electron recombination. Specifically, they exchange D1 subunits origi- transfers in PSII. However, the full scheme of electron transfer nating from different psbA genes to change the hydrogen bond regulation remains unresolved due to the unknown Em value of interactions of Pheo (16–20). On the other hand, it was found QB. Here, for the first time (to our knowledge), the Em value of QB that impairment of the Mn4CaO5 cluster led to a significant in- reduction was measured directly using spectroelectrochemistry in crease in the Em of QA by ∼150 mV (21–27).