bioRxiv preprint doi: https://doi.org/10.1101/2021.06.15.448481; this version posted June 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Complete metamorphosis and microbiota turnover in insects 2 3 Chrisitin Manthey1, Paul Johnston*2,3,4, Jens Rolff1,2,4 4 1. Freie Universität Berlin, Institut für Biologie, Evolutionary Biology, Königin-Luise-Strasse 1-3, 5 14195 Berlin, Germany. 6 2. Berlin Center for Genomics in Biodiversity Research, Berlin, Germany; 7 3. Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany; 8 4. Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB), Berlin, 9 Germany 10 *Paul Johnston, author for correspondence 11 *Jens Rolff, author for correspondence 12 13 14 Email:
[email protected], Jens.rolff@fu-berlin,de 15 Competing Interest Statement: The authors state that they have no competing interests. 16 Keywords: holometaboly, beta diversity, evolution of metamorphosis, gut microbiota 17 18 Abstract 19 The insects constitute the majority of animal diversity. Most insects are holometabolous: during 20 complete metamorphosis their bodies are radically re-organized. This re-organization poses a 21 significant challenge to the gut microbiota, as the gut is replaced during pupation, a process that 22 does not occur in hemimetabolous insects. In holometabolous hosts, it offers the opportunity to 23 decouple the gut microbiota between the larval and adult life stages resulting in high beta diversity 24 whilst limiting alpha diversity.