bioRxiv preprint doi: https://doi.org/10.1101/2020.12.08.414581; this version posted December 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE Takayuki Nagaea, Masashi Unnob, Taiki Koizumic, Yohei Miyanoirid, Tomotsumi Fujisawab, Kento Masuif, Takanari Kamof, Kei Wadae, Toshihiko Ekif, Yutaka ItoC, Yuu Hirosef and Masaki Mishimac aSynchrotron Radiation Research Center, Nagoya University, Chikusa, Nagoya 464-8603, Japan; bDepartment of Chemistry and Applied Chemistry, Faculty of Science and Engineering, Saga University, Saga 840-8502, Japan; cDepartment of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji 192-0397, Japan; dInstitute for Protein Research , Osaka University , 3-2 Yamadaoka , Suita , Osaka 565-0871 , Japan; eDepartment of Medical Sciences, University of Miyazaki, Miyazaki 889-1692, Japan; fDepartment of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan 1To whom correspondence should be addressed 1Masaki Mishima Email:
[email protected] 1Yuu Hirose Email:
[email protected] 1 Masashi Unno Email:
[email protected] Takayuki Nagae: 0000-0001-7016-5183 Tomotsumi Fujisawa: 0000-0002-3282-6814 Masashi Unno: 0000-0002-5016-6274 Yohei Miyanoiri: 0000-0001-6889-5160 Yuu Hirose: 0000-0003-1116-8979 Kei Wada: 0000-0001-7631-4151 Yutaka Ito: 0000-0002-1030-4660 Masaki Mishima: 0000-0001-7626-7287 Classification Biological Sciences Keywords cyanobacteriochrome, phytochrome, bilin, NMR, crystallography Author Contributions MM, YH, and MU designed the research and wrote the manuscript.