Sahar Abd EL-Mogheth Abd El-Ghaffar

Sahar Abd el-mogheth

Sahar Abd EL-mogheth Abd El-ghaffar

Abu tesht, 83661, qena, Egypt. +02-01063718038 – 00310653167565 [email protected]

Nationality Egyptian.

Objective

I aim in this phase of my life to invest all my experiences in a PhD program that could supply me with all the educational and academic virtues I need to pursue a career in medical Biotechnology, my science passion, for which I believe I have huge potentials. Conducted Researches.

 Exploring the antimicrobial, antioxidant and hepatoprotective effects of kefir; (A probiotic fermented milk). our work was about The efficacy of kefir fermented milk was investigated as a hepato-protective against CCl4 inducing liver toxicity and as a protective agent against kidney and spleen damages.

WORK EXPERIENCE

Nov. 2011 – Jan.2018 Molecular Biology Specialist Royal Lab (for Clinical human analyses)  Performing qPCR experiments related to all virological diseases  Performing qPCR experiments related to bacterial diseases  genetic diseases examinations using PCR  qPCR data analyst. http://royal-lab.net/en.html.

Dec. 2015 – Dec.2018 Teaching, Advanced Academy Training Center in Cairo, Egypt .  Supervision most of teaching & research related labs of undergraduate & postgraduate courses.  teaching course of Laboratory medical analysis .  Lab preparation; compounding reagents & solutions and preparing handouts required for lab experiments  Assisted in explaining and demonstrating the lab experiments to the students during the lab session, Assignment and quiz grading.

Sep. 2016 – Feb. 2017 Marketing Representative. Pcr kits import, technical support (Molecular Biology / betalab)  Marketing

Sahar Abd el-mogheth

 Sales promotion  Customer support Www.betalab-eg.com

December 2013 – 2015 Molecular Biology Analysit. Generation Lab (for human genetic disorders)  genetic diseases examinations using PCR, Hybridization, and qPCR. www.generations-labs.com

EDUCATION AND TRAINING

Sep 2017 Master's degree in medical Biotechnology , Faculty of Post Graduate Studies for Advanced Sciences, Beni Suef University. http://www.psas.bsu.edu.eg/Sector_Home.aspx?cat_id=%2718%27&lang=en

May 2014 Pre-Master in medical Biotechnology Faculty of Post Graduate Studies for Advanced Sciences, Beni Suef University Successfully completed the preparatory courses for Master’s degree. http://www.psas.bsu.edu.eg/Sector_Home.aspx?cat_id=%2718%27&lang=en

Sep 2010 Graduate Diploma in physiology and biochemistry - Faculty of Science - Suez Canal University - Ismailia- egypt . http://scuegypt.edu.eg/en/.

May 2009 Bachelor of Science, : Faculty of Science - SouthValleyUniversity. http://www.svu.edu.eg/.

May 2017 training course on JANUS G3 BioTx Pro Plus Workstation and ChemagicTM 360-D instrument for PerkinElmer’s. perkinelmer.com.

Mar,2016 Roche Molecular Diagnostics training course on COBAS® AmpliPrep Instrument to automate sample processing and COBAS® TaqMan® 48 Analyzer for automated amplification and detection. https://usdiagnostics.roche.com/en/index.html.

May 2015 " DTlite and DT prime" Real time PCR (DNA-technology,Russia).training including The following topics: - Installation , operation and troubleshooting. - single tube kits - magnetic beeds and vucuum columm technology - HTS , manual kits and robotics. - Diagnostics of infectionus diseases kits.

Sahar Abd el-mogheth

- human genetics and salivagene product line. - Bioinformatics and interpertations. http://dna-technology.ru/eng/.

May 2014 " lab turbo"( tiagen , Taiwan ) neuclic acid workstation . training including The following topics: - Installation , operation and troubleshooting. - single tube kits - magnetic beeds and vucuum columm technology - HTS , manual kits and robotics. - Diagnostics of infectionus diseases kits. - human genetics and salivagene product line. - Bioinformatics and interpertations. http://dna-technology.ru/eng/.

Computer skills ICDL,Excellent command of Windows, Microsoft-Office, Internet. & Languages Mother tongue(s) Arabic.

English language Fluent.

Publications sahar abd el mogheith abd el ghaffar, ahmed osama el-gendy, serageldeen sultan, khalid ali el-nesr. Exploring the Antimicrobial and Hepatoprotective Effects of Kefir; A Probiotic Fermented Milk JOURNAL OF PURE AND APPLIED MICROBIOLOGY, June 2017. Vol. 11 (2), p. 759-772. http://dx.doi.org/10.22207/JPAM.11.2.15.

Conf. & Posters  28th Annual conference of the Egyptian society of laboratory medicine Crucial partner in healthcare "April 8 – 10,2017 cairo international convention Center (CICC), cairo,Egypt.

 27th Annual conference of the Egyptian society of laboratory medicine Crucial partner in healthcare "April 8 – 10.,2016 cairo international convention Center (CICC), cairo,Egypt.

 1st International scientific conference for syndicate of medical laboratory specialists " Thefutur of Medical Analysis in Egypt " 10,may 2013 . Bibliotheca Alexandria .

Techniques employed.

 Molecular Biology techniques: o DNA and RNA extraction, cDNA synthesis.

Sahar Abd el-mogheth

o RT-PCR (real time pcr) o Pcr in diagnostics of infectious diseases. o Human Genetics, o Molecular Cloning. o Site Directed Mutagenesis. o Expression. o Yeast Cell Cultures.  Microbiology techniques: o Bacterial cultivation and characterization, viable count, antibiotic susceptibility testing, minimum inhibitory concentration (MIC) etc…  Basic Cell culture techniques.  Proteomic techniques: o Polyacrylamide gel electrophoresis (PAGE). o 2D PAGE. o AKTA protein purification. o ELISA  qPCR.  Library construction (genomic, fosmid and Next generation sequencing libraries)  Sequencing o Sanger Sequencing o Next Generation sequencers (Roche 454, Illumina MiSeq).