Sahar Abd el-mogheth Sahar Abd EL-mogheth Abd El-ghaffar Abu tesht, 83661, qena, Egypt. +02-01063718038 – 00310653167565 [email protected] Nationality Egyptian. Objective I aim in this phase of my life to invest all my experiences in a PhD program that could supply me with all the educational and academic virtues I need to pursue a career in medical Biotechnology, my science passion, for which I believe I have huge potentials. Conducted Researches. Exploring the antimicrobial, antioxidant and hepatoprotective effects of kefir; (A probiotic fermented milk). our work was about The efficacy of kefir fermented milk was investigated as a hepato-protective against CCl4 inducing liver toxicity and as a protective agent against kidney and spleen damages. WORK EXPERIENCE Nov. 2011 – Jan.2018 Molecular Biology Specialist Royal Lab (for Clinical human analyses) Performing qPCR experiments related to all virological diseases Performing qPCR experiments related to bacterial diseases genetic diseases examinations using PCR qPCR data analyst. http://royal-lab.net/en.html. Dec. 2015 – Dec.2018 Teaching, Advanced Academy Training Center in Cairo, Egypt . Supervision most of teaching & research related labs of undergraduate & postgraduate courses. teaching course of Laboratory medical analysis . Lab preparation; compounding reagents & solutions and preparing handouts required for lab experiments Assisted in explaining and demonstrating the lab experiments to the students during the lab session, Assignment and quiz grading. Sep. 2016 – Feb. 2017 Marketing Representative. Pcr kits import, technical support (Molecular Biology / betalab) Marketing 1 Sahar Abd el-mogheth Sales promotion Customer support Www.betalab-eg.com December 2013 – 2015 Molecular Biology Analysit. Generation Lab (for human genetic disorders) genetic diseases examinations using PCR, Hybridization, and qPCR. www.generations-labs.com EDUCATION AND TRAINING Sep 2017 Master's degree in medical Biotechnology , Faculty of Post Graduate Studies for Advanced Sciences, Beni Suef University. http://www.psas.bsu.edu.eg/Sector_Home.aspx?cat_id=%2718%27&lang=en May 2014 Pre-Master in medical Biotechnology Faculty of Post Graduate Studies for Advanced Sciences, Beni Suef University Successfully completed the preparatory courses for Master’s degree. http://www.psas.bsu.edu.eg/Sector_Home.aspx?cat_id=%2718%27&lang=en Sep 2010 Graduate Diploma in physiology and biochemistry - Faculty of Science - Suez Canal University - Ismailia- egypt . http://scuegypt.edu.eg/en/. May 2009 Bachelor of Science, : Faculty of Science - SouthValleyUniversity. http://www.svu.edu.eg/. May 2017 training course on JANUS G3 BioTx Pro Plus Workstation and ChemagicTM 360-D instrument for PerkinElmer’s. perkinelmer.com. Mar,2016 Roche Molecular Diagnostics training course on COBAS® AmpliPrep Instrument to automate sample processing and COBAS® TaqMan® 48 Analyzer for automated amplification and detection. https://usdiagnostics.roche.com/en/index.html. May 2015 " DTlite and DT prime" Real time PCR (DNA-technology,Russia).training including The following topics: - Installation , operation and troubleshooting. - single tube kits - magnetic beeds and vucuum columm technology - HTS , manual kits and robotics. - Diagnostics of infectionus diseases kits. 2 Sahar Abd el-mogheth - human genetics and salivagene product line. - Bioinformatics and interpertations. http://dna-technology.ru/eng/. May 2014 " lab turbo"( tiagen , Taiwan ) neuclic acid workstation . training including The following topics: - Installation , operation and troubleshooting. - single tube kits - magnetic beeds and vucuum columm technology - HTS , manual kits and robotics. - Diagnostics of infectionus diseases kits. - human genetics and salivagene product line. - Bioinformatics and interpertations. http://dna-technology.ru/eng/. Computer skills ICDL,Excellent command of Windows, Microsoft-Office, Internet. & Languages Mother tongue(s) Arabic. English language Fluent. Publications sahar abd el mogheith abd el ghaffar, ahmed osama el-gendy, serageldeen sultan, khalid ali el-nesr. Exploring the Antimicrobial and Hepatoprotective Effects of Kefir; A Probiotic Fermented Milk JOURNAL OF PURE AND APPLIED MICROBIOLOGY, June 2017. Vol. 11 (2), p. 759-772. http://dx.doi.org/10.22207/JPAM.11.2.15. Conf. & Posters 28th Annual conference of the Egyptian society of laboratory medicine Crucial partner in healthcare "April 8 – 10,2017 cairo international convention Center (CICC), cairo,Egypt. 27th Annual conference of the Egyptian society of laboratory medicine Crucial partner in healthcare "April 8 – 10.,2016 cairo international convention Center (CICC), cairo,Egypt. 1st International scientific conference for syndicate of medical laboratory specialists " Thefutur of Medical Analysis in Egypt " 10,may 2013 . Bibliotheca Alexandria . Techniques employed. Molecular Biology techniques: o DNA and RNA extraction, cDNA synthesis. 3 Sahar Abd el-mogheth o RT-PCR (real time pcr) o Pcr in diagnostics of infectious diseases. o Human Genetics, o Molecular Cloning. o Site Directed Mutagenesis. o Expression. o Yeast Cell Cultures. Microbiology techniques: o Bacterial cultivation and characterization, viable count, antibiotic susceptibility testing, minimum inhibitory concentration (MIC) etc… Basic Cell culture techniques. Proteomic techniques: o Polyacrylamide gel electrophoresis (PAGE). o 2D PAGE. o AKTA protein purification. o ELISA qPCR. Library construction (genomic, fosmid and Next generation sequencing libraries) Sequencing o Sanger Sequencing o Next Generation sequencers (Roche 454, Illumina MiSeq). 4 .