Environmental Genomics: Mechanisms and Approaches for Genomic Integrity

SEPTEMBER 12-16, 2020 VIRTUAL MEETING

ENVIRONMENTAL GENOMICS: MECHANISMS AND APPROACHES FOR GENOMIC INTEGRITY

Applied Genetic Toxicology Gentoxicity Risk and Public Health DNA Repair & Mutagenic Mechanisms (GRAPH) Epigenomics Germ Cell and Heritable Effects Genomics and Data Sciences In Vivo Mutagenesis

William Kaufmann, PhD EMGS President Joann B. Sweasy, PhD EMGS Program Chair Aishwarya Prakash, PhD New Investigator Co-Chair

KEYNOTE SPEAKERS Kari Stefansson, M.D. Joan A. Steitz, PhD Dr. Med. CEO of deCODE Genetics Sterling Professor of Molecular Biophysics and Biochemistry Yale School of Medicine Serena Nik-Zainal, PhD University of Cambridge www.emgs-us.org/p/cm/ld/fid=501

SPONSORS OF THE 51ST VIRTUAL ANNUAL MEETING Diamond Escher Family Fund US Food and Drug Administration Grant No. 1R13FD006702-02

Platinum Merck DNA Repair Fundamental and Molecular Mechanisms of Mutagenesis Reviews in Mutation Research Genetic Toxicology and Environmental Mutagenesis

Silver Bristol-Myers Squibb Gilead Sciences Research Institute for Fragrance Materials, Inc. (RIFM) Teijin Pharma Limited ILS, Inc.

Bronze New England Biolabs Nucleic Acids Research NAR Cancer

General National Institute of Environmental Health Sciences

3 51ST ANNUAL MEETING

Synergistic Interactions for a Better World

Environmental Genomics: Mechanisms and Approaches for Genomic Integrity

September 12-16, 2020

VIRTUAL MEETING

EMGS President: William Kaufmann, PhD EMGS Program Chair: Joann B. Sweasy, PhD New Investigator Co-Chair: Aishwarya Prakash, PhD

Environmental Mutagenesis and Genomics Society 12627 San Jose Blvd., Suite 202 | Jacksonville, Florida 32223 Telephone: 904.289.3410 | Fax: 904.513.2666 Email: [email protected] | EMGS Website: https://www.emgs-us.org

EMGS Program Abstracts printed in Environmental and Molecular Mutagenesis Volume 61, Number S1

The Environmental Mutagenesis and Genomics Society was founded in 1969 and is incorporated under the laws of the District of Columbia. Its purpose is to encourage the study of mutagens in the human environment, particularly as they may affect public health, and to engage in and sponsor research and the dissemination of information related to mutagens. Membership is open to all interested scientists. https://www.emgs-us.org/page/emgsmembership

4 Table of Contents

EMGS Sponsor List...... 3 Title Page...... 4 How to Access EMGS 2020...... 6 EMGS Leadership...... 7-9 Welcome, General Information...... 10 Sponsor Recognition...... 12-13 Keynote Speakers...... 14-15 Special Interest Groups & Committees at a Glance...... 16 EMGS Program Schedule...... 17-37 EMGS Posters...... 38-44

EMGS Annual Event Site

On the Annual Meeting Event site you can view the abstracts for all sessions, build your agenda, see a list of attendees, and more.

To access the event site, use the following links:

EMGS Annual Meeting Site: https://www.emgs-us.org/p/cm/ld/fid=501

Accessing Live Virtual Sessions: Presenters and registered attendees will receive an email with an invitation to the EMGS 2020 Virtual Annual Meeting. This will enable all users to access our Virtual Annual Meeting page that will have links to all the meetings as well as showcase our poster presentations. (See the planned Program for a thor- ough overview of the meeting.)

For other questions about the meeting, please contact the EMGS business office at [email protected] or call 904-289-3410.

The Program for the 2020 Meeting may be updated throughout the meeting. To see the Program, go to https://www.emgs-us.org/p/cm/ld/fid=520

5 How To Access EMGS 2020

The Environmental Mutagenesis and Genomics Society’s Virtual Meeting kicks off September 12. Ev- ery presenter and registrant should be familiar with 3 KEY SITES for attending the meeting. Please read carefully and refer to this page as you prepare for and attend EMGS 2020. 3 KEY SITES

1) EMGS Website https://www.emgs-us.org/p/cm/ld/fid=501

This is the site for finding an overview of EMGS 2020 and its offerings including the official Program. https://www.emgs-us.org/p/cm/ld/fid=501

2) EMGS 2020 Official Event Site https://www.emgs-us.org/event/15/

Ready to plan your week? Start here. Registered attendees can browse scheduled programs and sessions, and review information about the presenters and abstracts. You can explore what is on the schedule, day by day, and then save specific presentations to a personalized agenda (to be found under your “My Profile” tab.) NOTE: This site requires a login, so have access to the email you used to register for the meeting. Your MEETING REGISTRATION CODE (which you can find in your registration confirmation email) will serve as your password for your initial login.Y ou can update that later.

3) VIRTUAL SESSION SITE https://www.emgs2020conference.com

This is where you will access live and recorded sessions scheduled for EMGS 2020. The Virtual An- nual Meeting Site — https://www.emgs2020conference.com/ — is now open and accessible to meeting registrants. This site is where you will access all of the meeting’s live and recorded content.

Please take the opportunity to log on and explore the Virtual Annual Meeting Site prior to the Septem- ber 12 kickoff. Once you log on, you will have access to an agenda for each day of the meeting.As scheduled presentations begin, you will have the option of clicking a “Join Here” button that will link you to a corresponding Zoom session.

For questions about the meeting, please contact the EMGS business office at [email protected] or call 904-289-3410.

6 EMGS Leadership EMGS Executive Board EMGS Council

President: William Kaufmann, PhD Bret Freudenthal (2021) Vice President: Carol Yauk, PhD Brian Chorley, PhD (2021) Treasurer: Christi A. Walter, PhD Caren Weinhouse, PhD (2021) Secretary: Janice M. Pluth, PhD Carol Swartz, PhD (2020) Past President: Miriam C. Poirier Chris Farabaugh, PhD (2020) Council Representative: Les Hanakahi Jill Escher, PhD (2022) Council Representative: Christopher Faulk Jackie Goodrich, PhD (2021) Chris Faulk, PhD (2022) Joel N. Meyer, PhD (2020) Les Hanakahi, PhD (2022) Nan Mei, PhD (2020) Natalie Gassman, PhD (2021) Robert Heflich, PhD (2022) Patricia L. Opresko, PhD (2020) Isabelle R. Miousse, PhD (2022)

EMGS Past Presidents Alexander Hollaender, PhD (1970) R. Julian Preston, PhD (1989) Peter J. Stambrook, PhD (2005) Ernst Freese, PhD (1972) George R. Hoffmann, PhD (1990) Martina L. Veigl, PhD (2006) Frederick J. de Serres, PhD (1974) Michael D. Waters, PhD (1991) Andrew J. Wyrobek, PhD (2007) Jan Drake, PhD (1976) James T. MacGregor, PhD, DABT (1992) Priscilla K. Cooper, PhD (2008) Seymour Abrahamson, PhD (1977) Philip C. Hanawalt, PhD (1993) Michael J. Plewa, PhD (2009) David J. Brusick, PhD, ATS (1978) James M. Gentile, PhD (1994) Jeffrey L. Schwartz, PhD (2010) Mortimer L. Mendelsohn, MD, PhD (1979) Raymond R. Tice, PhD (1995) Catherine B. Klein, PhD (2011) Sheldon Wolff, PhD (1980) Raymond W. Tennant, PhD (1996) Mats Ljungman, PhD (2012) Verne A. Ray, PhD (1981) Sid Aaron, PhD, DABT (1997) Ofelia A. Olivero, PhD, ATS (2013) Gordon W. Newell, PhD (1982) Rosalie K. Elespuru, PhD (1998) Suzanne M. Morris, PhD (2014) Richard J. Albertini, MD, PhD (1983) James S. Felton, PhD (1999) Bevin P. Engelward, ScD (2015) Liane B. Russell, PhD (1984) James D. Tucker, PhD (2000) Thomas E. Wilson, MD, PhD (2016) Michael D. Shelby, PhD (1985) David M. DeMarini, PhD (2001) Robert W. Sobol, PhD (2017) John A. Heddle, PhD (1986) Lawrence A. Loeb, MD, PhD (2002) Miriam C. Poirier, PhD (2018) Anthony V. Carrano, PhD (1987) David A. Eastmond, PhD (2003) William Kaufmann, PhD (2019) Sheila M. Galloway, PhD (1988) Leona D. Samson, PhD (2004)

EMGS Staff Robert Bevans-Kerr, Executive Director Janece Bevans-Kerr, Director of Member Services Nathan Bevans-Kerr, Executive Assistant Diana Kerr, Executive Assistant Craig Richardson, Assistant Manager Channah Pool, Executive Assistant

7 EMGS Committees Alexander Hollaender Outreach Finance and Fundraising Patricia A. Escobar, PhD, Chair Barbara S. Shane, PhD, DABT, Chair Emilio Rojas, PhD, Co-Chair Laura L. Custer, PhD, Treasurer Brian N Chorley, PhD, Council Liaison Robert A. Bevans-Kerr, Executive Director Graciela Spivak, PhD, Past Chair Patricia L. Opresko, PhD, Council Liaison Priscilla K. Cooper, PhD Malathi Banda, PhD Philip C. Hanawalt, PhD Thomas W. Glover, PhD Mats Ljungman, PhD Guo-Min Li Patricia Ramos-Morales, DSc Jeffrey L. Schwartz, PhD Javier Revollo, PhD Robert W. Sobol Hilde E. van Gijssel, PhD Robert R. Young, MS Paul A. White, PhD Luoping Zhang, PhD Membership Professional Development Stephanie L. Smith-Roe, PhD, Chair Awards & Honors Malathi Banda, PhD, Co-Chair Michael George Kemp, PhD, Chair Nan Mei, PhD, Council Liaison Nan Mei, PhD, Co-Chair Julie Cox Isabelle R. Miousse, PhD, Board Liaison Hilde E. van Gijssel, PhD William M. Baird, PhD Xiaoqing Guo Nikolai Chepelev, PhD Catherine B. Klein, PhD Shobhan Gaddameedhi, PhD Maxwell C.K. Leung, PhD Kathleen A. Hill, PhD John J. Nicolette, MS, DABT Mugimane G. Manjanatha, PhD Jonathan B. Ward, Jr., PhD Hilde E. van Gijssel, PhD Shan Yan, PhD Nominating Miriam C. Poirier, PhD, Chair Annual Meeting Publicity Bevin P. Engleward, ScD XXX Charles Farabaugh, PhD Daniel J. Roberts, MS Diversity & Inclusion Hilde E. van Gijssel, PhD Kelly Harris, PhD, Chair Caren Weinhouse, PhD, MPH Jackie M. Goodrich, PhD, Council Liaison David Michael DeMarini, PhD Program Bevin P. Engelward, ScD Joann B. Sweasy, PhD, Program Chair Les Hanakahi, PhD Aishwarya Prakash, PhD, Program Young Investigator Zachary D. Nagel, PhD Chris Farabaugh, PhD, Council Liaison Ofelia Ana Olivero, PhD, ATS William Kaufmann, PhD, EMGS President/Past Program Chair Hilde E. van Gijssel, PhD Carole L. Yauk, PhD, EMGS Vice President Robert R. Young, MS Miriam C. Poirier, PhD, EMGS Past President Jonatan Axelsson, MD, PhD Education, Student and New Investigator Affairs Jeffrey Craig Bemis, PhD Jeffrey Craig Bemis, PhD, Co-Chair Robert A. Bevans-Kerr, EMGS Executive Director Elyse Bolterstein, PhD, Co-Chair Brian N Chorley, PhD Amy Michelle Whitaker, PhD, New Investigator Co-Chair Rosalie K. Elespuru, PhD Nan Mei, PhD, Council Liaison Zachary D. Nagel, PhD Malathi Banda, PhD Barbara L. Parsons, PhD Guosheng Chen, PhD Mary Margaret Pratt, PhD Les Hanakahi, PhD Mitchell Turker, PhD Catherine B. Klein, PhD Mark Wilson, PhD Alexandra S. Long, PhD Robert R. Young, MS Isabelle R. Miousse, PhD Suzanne M. Morris, PhD Meagan Myers, PhD Janice M. Pluth, PhD Stephanie L. Smith-Roe, PhD Carole L. Yauk, PhD 8 EMGS News Page B. McKinzie, PhD, Chair Special Interest Groups Caren Weinhouse, PhD, MPH, Council Liaison Arijit Dutta, PhD Applied Genetic Toxicology Meagan Myers, PhD Alexandra S. Long, PhD, Chair Carlos A. Torres-Ramos, PhD Daniel J. Roberts, MS, Co-Chair Shan Yan, PhD Eunnara Cho, Young Investigator Co-Chair

White Paper & Positions DNA Repair & Mutagenic Mechanisms Natalie Rose Gassman, PhD, Council Liaison Bret David Freudenthal, PhD, Co-Chair Peter Sykora Zachary D. Nagel, PhD, Co-Chair Kristine L. Witt, MS Errol Zeiger, PhD, JD, ATS Epigenomics Jackie M. Goodrich, PhD, Chair Publication Policy Isabelle R. Miousse, PhD, Co-Chair Kristine L. Witt, MS, Chair Sumira Phatak, Student Co-Chair E. Maria Donner, PhD, Co-Chair Errol Zeiger, PhD, JD, ATS, Past Chair Genomics SIG Natalie Rose Gassman, PhD, Council Liaison Thomas E. Wilson, MD, PhD, Chair B. Bhaskar Gollapudi, PhD, EMM Editor in Chief Christopher Faulk, PhD, Co-Chair David M. Wilson, III, PhD, EMM Associate Editor Tess C. Leuthner, New Investigator Co-Chair Ulla Birgitte Vogel, PhD, EMM Associate Editor Stephen D. Dertinger, PhD Gentoxicity Risk Assessment and Public Health (GRAPH) David A. Eastmond, PhD Catherine Gibbons, PhD, Co-Chair Robert H. Heflich, PhD Mary Margaret Pratt, PhD, Co-Chair George R. Hoffmann, PhD Francesco Marchetti, PhD Germ Cell and Heritable Effects Joel N. Meyer, PhD Jill Escher, MA, JD, Chair Barbara L. Parsons, PhD Jonatan Axelsson, MD, PhD, Co-Chair Paul A. White, PhD Mathia L. Colwell, Young Investigator Co-Chair David M. Wilson III, PhD, Journal Editor Shan Yan, PhD In Vivo Mutagenesis Francesco Marchetti, PhD, Co-Chair Women in EMGS Mark Fielden, PhD, Co-Chair Meagan Myers, PhD, Chair Charles C. Valentine, III, M.Sc., Young Investigator Aishwarya Prakash, PhD, Co-Chair Special Interest Group Leaders Retired/Emeritus Group Catherine B. Klein, PhD, Co-Chair Jonathan B. Ward, Jr., PhD, Chair Meagan Myers, PhD, Co-Chair

New Directions Task Force Miriam C. Poirier, PhD, Chair EMGS Endowment Board David Michael DeMarini, PhD Stephen D. Dertinger, PhD Catherine B. Klein, PhD, Chair Bevin P. Engelward, ScD Jack B. Bishop, PhD Patricia A. Escobar, PhD John J. Nicolette, MS, DABT William Kaufmann, PhD Robert H. Schiestl, PhD Aishwarya Prakash, PhD Robert W. Sobol, PhD Stephanie L. Smith-Roe, PhD Michael D. Waters, PhD Leon F. Stankowski, Jr., PhD Joann B. Sweasy, PhD Jonathan B. Ward, Jr., PhD Paul A. White, PhD Carole L. Yauk, PhD Errol Zeiger, PhD, JD, ATS

9 Welcome to the 2020 Annual Meeting

Welcome to the 51st annual meeting of the Environmental Mutagenesis and Genomics Society. We hope you enjoy the meeting, which is designed to provide access to cutting-edge science as well as opportunities for socializing with friends and colleagues.

Your Program Committee has assembled a large cast of Keynote Speakers, Symposia, Platform Pre- sentations and Poster Sessions to inform, challenge and educate.

To be successful in the future EMGS needs your participation, so please sign up for a Committee and attend Special Interest Group (SIG) meetings.

Enjoy the meeting and please join us to help impact global health!

Joann B. Sweasy, EMGS 2020 Program Chair

William Kaufmann, EMGS 2020 President General Information

The EMGS Annual Meeting uniquely brings together leading scientists from academia, industry, and government to discuss cutting-edge research aimed at understanding and mitigating environmental threats to the genome and to the epigenome. Environmental exposures pose a complex and constantly evolving threat to genomic integrity, putting our children and ourselves at increased risk of disease.

Our dynamic and interactive meetings are designed to promote the Society’s mission to foster scientific research, education, and collaboration with a focus on issues pertinent to genomic stability. Emphasis areas include DNA damage, DNA repair, mutagenesis, heritable effects, and epigenetic alterations in genome function.

Additionally, the EMGS aims to promote the application and communication of knowledge relevant to genetic toxicology testing, risk assessment, and regulatory policy-making to protect human health and the environment.

For decades, the EMGS Annual Meeting has been creating opportunities for new investigators to join a welcoming community of scientists working in research institutions, regulatory agencies, and industry, all with a shared commitment toward public health. The Society puts a major focus on supporting the next generation of scientists through both formal and informal activities throughout the meeting.

The EMGS Welcomes You

The EMGS recognizes the scientific achievements of our diverse membership, and fosters career development and advancement for everyone. We are committed to supporting all members of the EMGS community, irrespective of age, culture, ability, race, ethnicity, nationality, gender identity and expression, sexual orientation, marital status, religious affiliation or socioeconomic status. For questions, comments, or concerns, please contact the Committee for Diversity and Inclusion. https://www.emgs-us.org/page/diversity

10 Meetings & Poster Hours

EMGS Committee Meetings

The Environmental Mutagenesis and Genomics Society has a number of committees which are pop- ulated by EMGS members. Participation at the committee level is a great opportunity to get involved with EMGS and engage with the leadership. Committees have contributed significantly to the growth of EMGS through their oversight and development of new programs. The EMGS committees are active throughout the year and meet during the conference. The committee meeting dates and times are noted in the agenda. Members are encouraged to be involved in a committee. Contact the committee chair if you are interested in serving on a committee.

EMGS Special Interest Groups

The Special Interest Groups represent the scientific diversity of EMGS, and SIG meetings are a time-tested favorite of the Annual Meeting. The format provides free-form discussions and short presentations of the key challenges and research needs of the interest area. The SIGs provide a casual way for young investigators and seasoned researchers to interact.

Business Meetings

The Business Meeting will take place on Wednesday, September 16, from Noon to 1:30 p.m. CDT. All EMGS Members and interested EMGS Members are encouraged to attend.

Author-Attended Poster Sessions

Poster Session Q&As will be held Saturday, September 12. Presentations are categorized by topic into one of nine 30-minute blocks that will begin at 8:00 a.m. CDT and conclude at 2:30 p.m. CDT. To see scheduled presentation times, go to https://www.emgs-us.org/p/cm/ld/fid=535.

of the Environmental Mutagenesis and Genomics Society will Program Overview provide a venue for stimulating cross-disciplinary research and discovery. We will learn how environmental agents impact genom- Environmental toxins and toxicants impact human physiology and ic, proteomic and metabolic signatures, and how we can use this biochemistry by altering or modifying DNA, RNA, proteins and knowledge base to develop informed biomarkers and improved metabolites. Across the spectrum of scientific inquiry, we are chal- regulatory policies that address human health concerns related to lenged with understanding the mechanisms which respond to or environmental exposures. modulate these genotoxic effects. Furthermore, the development of tools for analysis, as well as, for evaluation of these biological Join us as we cross this bridge, find common ground in our ad- endpoints are critical for the promotion and protection of human vances in the study of Environmental Health Sciences, and bring health. By forging a bridge to bring scientists studying exposures, together our efforts on exposure, mechanism and its impact on mechanisms, and regulatory sciences, the 2020 Annual Meeting public health.

11 EMGS 2020 SPONSORS

12 EMGS 2020 SPONSORS

NAR Cancer

13 KEYNOTE

Joan A. Steitz, PhD Kári Stefánsson, PhD Serena Nik-Zainal, PhD Yale School of Medicine Dr. Med. CEO of deCODE Genetics University of Cambridge

JOAN A. STEITZ, PhD YALE SCHOOL OF MEDICINE

As a college student in the 1960s, Joan Steitz never imagined herself as a top-flight scientist. Certainly, she was fascinated by science. She even assisted senior scientists in laboratories at the Massachusetts Institute of Technology, where she was befriended by James D. Watson, co-discoverer of the DNA double helix, and at the Max Planck Institute in Germany. But when it came time to choose a career path, she had never seen a female professor or head of lab, so she never aspired to such goals. Today, Prof. Joan Steitz is one of leading scientists in her field. Steitz is best known for her pioneer- ing work in RNA. She and her student, Michael Lerner, discovered and defined the function of small ri- bonucleoproteins (snRNPs) in pre-messenger RNA — the earliest product of DNA transcription — and was the first to learn that these cellular complexes (snRNPs) play a key role in processing messenger RNA by excising noncoding regions and splicing together the resulting segments. Her breakthroughs into the previously mysterious splicing process have clarified the science behind the formation of proteins and other biological processes, including the intricate changes that occur as the immune system and brain develop. Steitz earned her PhD from Harvard in 1967. After completing postdoctoral work in Cambridge, England, she joined the Department of Molecular Biophysics and Biochemistry at Yale as an assistant professor and later became an associate and full professor, as well as chair of the department.

14 KÁRI STEFÁNSSON, PhD DR. MED. CEO OF DECODE GENETICS

Kári Stefánsson, M.D., Dr. Med., founded deCODE in August 1996. Dr. Stefánsson was previously a professor of Neurology, Neuropathology and Neuroscience at Harvard University and Director of Neuropathology at Beth Israel Hospital in Boston, Massachusetts. From 1983 to 1993, he held faculty positions in Neurology, Neuropathology and Neurosciences at the University of Chicago. Dr. Stefánsson received his M.D. and Dr. Med. from the University of Iceland and is board-certified in neurology and neuropathology in the United States. Dr. Stefansson is recognized as a leading figure in human genetics. He has shaped deCODE’s scientific approach and been actively engaged in leading its gene discovery work, serving as senior author on most of the company’s publications in major scientific journals.

SERENA NIK-ZAINAL, PhD UNIVERSITY OF CAMBRIDGE

Dr. Serena Nik-Zainal is a CRUK Advanced Clinician Scientist and an Honorary Consultant in Clinical Genetics. Nik-Zainal qualified in medicine from the University of Cambridge in 2000 on a scholarship from Petronas, Malaysia. She undertook a PhD at the Wellcome Sanger Institute (WSI) in 2009 exploring breast cancer using whole genome sequencing (WGS). She demonstrated how detailed downstream analyses of all mutations present in WGS breast cancers could reveal mutation signatures, imprints left by mutagenic processes that have occurred through cancer development. She also identified a novel phenomenon of localized hypermutation termed “kataegis”. Nik-Zainal was awarded a Wellcome Trust Intermediate Clinical Fellowship in 2013. She joined the Sanger Institute faculty team in 2014 and continued to develop particular expertise in the analysis and interpretation of WGS tumors. Apart from using computational approaches, she also studies mutational signatures experimentally using cell-based model systems. She was awarded a CRUK Advanced Clinician Scientist Fellowship in 2017 and moved to the University of Cambridge in order to accelerate the translation of her genomics expertise towards clinical applications and to further her work into the physiological mechanisms underpinning mutagenesis.

KEYNOTE 01 Sunday, September 13, 2020 | 11:00 AM - 12:00 PM CDT A Compendium of Mutational Signatures of Environmental Agents in Human Cancers and Normal Cells Serena Nik-Zainal, CRUK Advanced Clinician Scientist and Honorary Consultant in Clinical Genetics, University of Cambridge

KEYNOTE 02 Monday, September 14, 2020 | 11:00 AM - 12:00 PM CDT The Generation of New Diversity in the Sequence of the Human Genome Kári Stefánsson, M.D., Dr Med, deCODE Genetics, Iceland

KEYNOTE 03 Tuesday, September 15, 2020 | 11:00 AM - 12:00 PM CDT The Enigma of Viral Noncoding RNAs Joan A. Steitz, PhD, Sterling Professor of Molecular Biophysics and Biochemistry, Yale School of Medicine SPEAKERS 15 SpecialSPECIAL Interest INTEREST Group/Committee GROUP/COMMITTEE MEETINGS Meetings AtAT A A GlanceGLANCE

Thursday, September 10, 2020 3:00 PM - 4:30 PM EDT | EMGS Executive Board Meeting

Sunday, September 13, 2020 12:00 PM - 1:30 PM CDT | EMM Editorial Board 7:00 PM - 8:30 PM CDT | In Vivo Mutagenesis Special Interest Group 7:00 PM - 8:30 PM CDT | DNA Repair Special Interest Group 7:00 PM - 8:30 PM CDT | Epigenomics Special Interest Group

Monday, September 14, 2020 7:00 PM - 8:30 PM CDT | Genomics and Data Science Special Interest Group 7:00 PM - 8:30 PM CDT | GRAPH Special Interest Group

Tuesday, September 15, 2020 7:00 PM - 8:30 PM CDT | Applied Genetic Toxicology Special Interest Group 7:00 PM - 8:30 PM CDT | Germ Cell and Heritable Effects Special Interest Group

Wednesday, September 16, 2020 Committee Meetings 11:00 AM - 12:00 PM CDT | Awards and Honors Committee 11:00 AM - 12:00 PM CDT | Membership/PD Committee 11:00 AM - 12:00 PM CDT | Finance/Fundraising Committee 11:00 AM - 12:00 PM CDT | Hollaender Outreach Committee

12:00 PM - 1:30 PM CDT | EMGS Business Meeting Committee Meetings 4:00 PM - 5:00 PM CDT | Publication Policy Committee 4:00 PM - 5:00 PM CDT | Program Committee 4:00 PM - 5:00 PM CDT | Public Relations & Communications Committee 4:00 PM - 5:00 PM CDT | SIG Chairs Committee

Thursday, September 24, 2020 2:00 PM - 5:00 PM CDT | EMGS Council Meeting

16 EMGS Virtual Program

Thursday, September 10, 2020 3:00 PM - 4:30 PM EDT | EMGS Executive Board Meeting

Saturday, September 12, 2020 Poster Sessions 8:00 AM - 2:30 PM CDT | Poster Sessions Q&A will be held Saturday, September 12. Presentations are categorized by topic into one of nine 30-minute blocks that will begin at 8:00 a.m. CDT and conclude at 2:30 p.m. CDT. Click "EMGS Poster Sessions" Button to see presentation times.

EMGSEMGS POSTERPoster SESSIONS Sessions

Sunday, September 13, 2020 Symposium 01 8:00 AM - 10:00 AM CDT | To the Stars and Back Again- Studies in Space Radiation Research Chair: Janice Pluth, Co-Chair: Susan Bailey, Young Investigator: TBD The recently reported findings from NASA’s Twin Study and first One Year Mission brought much attention to the exciting research being done to better understand human health effects of long duration space flight. Certainly, induction of DNA damage and genomic instability are of concern, however little is known in regard to such responses resulting from chronic exposure to space radiations and the space environment.

8:00 AM – 8:25 AM CDT | Twins & Telomeres in Space Susan M. Bailey, Colorado State University 8:25 AM – 8:50 AM CDT | The Landscape of DNA and RNA Methylation Before, During, and After Human Space Travel Christopher E. Mason, Ph.D, Weill Cornell Medicine, Department of Physiology and Biophysics 8:50 AM – 9:25 AM CDT | Big Data Profiling of Health and Stress Periods Michael Snyder, Ph.D, Stanford University School of Medicine 9:25 AM – 9:30 AM CDT | Questions

9:30 AM – 9:45 AM CDT | Genetics and Radiation Exposure Regimen During Puberty Influence Mammary Organ Defects Janice M. Pluth, Ph.D, University of Nevada, Las Vegas 9:45 AM – 10:00 AM CDT | Side Effects of Scattered Versus Scanned Proton Beams on Normal Tissues In Total Body Irradiated Mice: Preliminary Results Samia Chaouni, ABTE Laboratory / University of Caen

17 Symposium 02 8:00 AM - 10:00 AM CDT | Modernizing the Genotoxicity Testing Paradigm: How Adverse Outcome Pathways, Integrated Approaches to Testing and Assessment (IATA), and Quantitative Analyses in Genetic Toxicology are Impacting Modern Risk Assessment Practices Chair: Alexandra Long, Co-Chair: Stefan Pfuhler, Young Investigator: Eunnara Cho The genotoxicity testing paradigm is transitioning from in vivo testing towards predictive in vitro and in silico methods, and quantitative genetic toxicology applications. AOPs provide a framework for documenting the biological knowledge and empirical data to describe the key events leading to adverse outcomes. Quantitative modeling is required to understand the relationship between key events and build predictive toxicology models from AOPs, and identify points of departure. AOPs can be leveraged to build test paradigms that implement the newest (non-test guideline) test methods for regulatory decision-making. One approach to efficiently increase application of these novel concepts in risk assessment is through the development of Integrated Approaches to Testing and Assessment (IATA). This symposium will provide: 1) an introduction to AOPs and IATAs, recent advances in these programs, and examples of how they are impacting risk assessment practices; 2) a synthesis of the AOP projects ongoing within the Health and Environmental Sciences Institute’s (HESI) Genetic Toxicology Technical Committee (GTTC) and their expected applications; 3) advances in the use of genetic toxicity dose-response data including discussions on critical effects sizes and uncertainty factors applied to points of departure; 4) application of the IATA concept in integrated genotoxicity testing by Health Canada in their GeneTox21 program; 5) the use of AOPs in the development of predictive toxicology models. Collectively, this symposium will provide an overview of different collaborative efforts to modernize genotoxicity assessment through the use of AOPs, IATA and genotoxicity dose-response analysis.

8:00 AM – 8:25 AM CDT | AOPs and IATA: What They Are and Why They Should Matter to the EMGS Jason O’Brien, Environment and Climate Change Canada 8:25 AM – 8:50 AM CDT | The Health and Environmental Institutes Genetic Toxicology Technical Committee AOP initiative: The Growing Genotoxicity AOP Network and Practical Applications Stefan Pfuhler, Procter & Gamble 8:50 AM – 9:15 AM CDT | Harnessing Genomics and AOPs for Predictive Toxicology Edward Perkins, US Army Corps of Engineers 9:15 AM – 9:40 AM CDT | GeneTox21: An Integrated In Vitro Genetic Toxicity Assessment Platform for the 21st Century Joleen Hanna, Health Canada 9:40 AM – 10:00 AM CDT | Quantitative Analysis of In Vivo Mutagenicity Dose-Response Data for Risk Assessment and Regulatory Decision-making: A Case Study of Alkylnitrosamines George Johnson, Swansea University Medical School (UK)

18 Symposium 03 8:00 AM - 10:00 AM CDT | The Toxicology of E-Cigarettes Co-Chairs: Catherine Gibbons, Dan Roberts Young Investigator: Esther Omaiye Moderator: Les Recio The widespread use of e-cigarettes, particularly among young people, and growing public health concerns are accompanied by an increasingly discordant message regarding the evidence of toxicity presented by academia and industry. A discussion of the existing toxicity and genotoxicity data, and recommendations for future testing needs, will help clarify actual risk and help inform regulatory decision-making. Speakers will present current evidence of the toxic and genotoxic effects of e-cigarettes; the end of the session will provide time for an open and objective facilitated discussion on what is known regarding the toxicity of e-cigarettes.

8:00 AM – 8:25 AM CDT | Chemical Elements and Metals in Aerosols from Three Generations of Electronic Cigarettes Monique Williams, University of California, Riverside 8:25 AM – 8:50 AM CDT | Technical Issues Involved with In Vitro Genotoxicity Testing of e- Cigarette Formulations Leon Stankowski, Charles River Laboratories 8:50 AM – 9:15 AM CDT | Toxicology of e-cigarettes Daniel Smart, Philip Morris International 9:15 AM – 9:40 AM CDT | E-cigarettes: The FDA perspective Priscilla Callahan-Lyon, US FDA, Center for Tobacco Products 9:40 AM – 10:00 AM CDT | E-cigarettes and Vaping: Toxicities, Misconceptions, and Health Effects Ilona Jaspers, University of North Carolina

10:00 AM - 11:00 AM CDT | Break

Keynote 01 11:00 AM - 12:00 PM CDT | A Compendium of Mutational Signatures of Environmental Agents in Human Cancers and Normal Cells Serena Nik-Zainal, CRUK Advanced Clinician Scientist and Honorary Consultant in Clinical Genetics, University of Cambridge

12:00 PM - 1:30 PM CDT | Break

12:00 PM - 1:30 PM CDT | EMM Editorial Board

19 Symposium 04 1:30 PM - 3:30 PM CDT | Nucleosome Dynamics and Environmental Stress Chair: Kara Bernstein, Co-Chair: TBD, Young Investigator: Heather O’Hagan Our cells are constantly responding the onslaught of environmental toxicants that threaten the integrity of our genome. This symposium entitled “Nucleosome Dynamics and Environmental Stress” will address current topics of how our environmental exposures influence nucleosome dynamics leading to different repair outcomes. We will cover different types of DNA damage and how individual DNA repair pathways are engaged to ensure genomic integrity focusing on oxidative damage, mismatch repair, and chromatin remodeling complexes.

1:30 PM – 2:00 PM CDT | Mismatch Repair Proteins Initiate Epigenetic Alterations during Inflammation-Driven Tumorigenesis Heather O’Hagan, Indiana University School of Medicine 2:00 PM – 2:30 PM CDT | Epigenetic Regulation of Carcinogen Susceptibility Ashby Morrison, PhD, Stanford University 2:30 PM – 3:00 PM CDT | Global Repair Fingerprinting of Glycosylase Activity on Nucleosomes Sarah Delaney, PhD, Brown University 3:00 PM – 3:15 PM CDT | Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A is Mediated by RPA Shruthi Sriramkumar, Indiana University School of Medicine 3:15 PM – 3:30 PM CDT | Cohesin SA1 and SA2 are RNA Binding Proteins That Localize to RNA Containing Regions on DNA Hong Wang, North Carolina State University

Symposium 05 1:30 PM - 3:30 PM CDT | Mitochondria: Environment, Epigenetics, and Disease Chair: Aishwarya Prakash, Co-Chair: TBD, Young Investigator: Laurie H. Andolina Over the past few decades mitochondria have emerged as more than just the powerhouse of the cell. Mitochondrial DNA, like its nuclear counterpart is damaged by both environmental and endogenous agents. Aberrant mitochondrial DNA metabolism leads to a myriad of diseases including aging related neurological disorders. This symposium entitled “Mitochondria: Environment, Epigenetics, and Disease” will address current topics of how onslaught to mtDNA influences epigenetic changes and mitochondrial disease. 1:30 PM – 2:00 PM CDT | Mitchondrial DNA Stress Protects the Nuclear Genome Gerald S. Shadel, Salk Institute for Biological Studies 2:00 PM – 2:30 PM CDT | Ultrasensitive Deletion Detection Links Mitochondrial Replication, Disease And Aging Bill Copeland, National Institute of Environmental Health Sciences 2:30 PM – 3:00 PM CDT | (Epi)genomics Effects of Developmental Mitochondrial Dysfunction Janine Santos, National Institute of Environmental Health Sciences 3:00 PM – 3:15 PM CDT | Mitochondria: Environment, Epigenetics, and Disease Parminder Kaur, North Carolina State University

20 3:15 PM – 3:00 PM CDT | Analysis of Illumina 450K DNA Methylation in NEST Cord Blood Reveals Sex Differences at Mitochondrial Genes in the Nuclear Genome Dillon King, Duke University

Symposium 06 1:30 PM - 3:30 PM CDT | Personalized Cancer Risk and Prevention: Are We Ready? Chair: Rosalie Elespuru, Co-Chair: Miriam Poirier, Young Investigator: TBD It has been known for some time that both genes and environment are factors in human cancer development. In the era of personal genomics, what is the likelihood that we can begin to assess personalized cancer risk, instead of overall cancer risk? A default cancer risk assessment as practiced today includes “uncertainty factors” to account for differential susceptibility, sensitivity, and other unknowns and variables within the human population. Assessment of cancer risk in general is a common goal, but there is information currently available to address cancer risk for specific cancers. Cancers for which genetic, environmental, viral and other associations are well established and could be used in personalized cancer risk assessments include colon, liver, head and neck, and lung cancer. This symposium includes a general overview of the potential for personalized cancer risk and presentations exploring the chemical, genetic, epigenetic, viral, dietary, exercise, and other environmental factors known for specific involvement in the development of colon, liver, and other cancers in humans. 1:30 PM – 2:00 PM CDT | Introduction: Evolution of Cancer Risk Assessment from Percival Pott to Personalized Cancer Prevention Rosalie Elespuru, US Food and Drug Agency 2:00 PM – 2:30 PM CDT | Contribution of Lifestyle and Environmental Exposures to Cancer: The Example Of Hepatocellular Carcinoma Farhad Islami, American Cancer Society 2:30 PM – 3:00 PM CDT | Cancer Prevention & Screening: The Next Step in the Era of Precision Medicine Asad Umar, National Cancer Institute 3:00 PM – 3:15 PM CDT | Effect of Exercise on DNA Damage and Repair Capacity Among African American Breast Cancer Survivors Rabindra Roy, Georgetown University 3:15 PM – 3:30 PM CDT | The Burden and Signatures of Somatic Mutations in Healthy Individuals Natalie Saini, NIEHS, NC

3:30 PM - 4:00 PM CDT | Break

Holleander Award Lecture 4:00 PM - 5:00 PM CDT | Source and Hazards of Environmental Mutagens: From Complex Mixture Analyses to Quantitative Dose-response Interpretation Paul A. White, Ph.D., Environmental Health Science and Research Bureau, Mechanistic Studies Division

21 5:00 PM - 7:00 PM CDT | Break

Special Interest Groups 7:00 PM - 8:30 PM CDT | In Vivo Mutagenesis 7:00 PM - 8:30 PM CDT | DNA Repair 7:00 PM - 8:30 PM CDT | Epigenomics

Monday, September 14, 2020 Symposium 07 8:00 AM - 10:00 AM CDT | Inflammation and Genomic Instability Chair: Joann Sweasy, Co-Chair: Bevin Engelward, Young Investigator: TBD Recent findings have emerged that suggest an interplay between genomic instability and inflammation. Genomic instability drives inflammation but inflammation is also suspected to induce genomic instability that leads to cancer. The presentations in this symposium by leading experts in the field will present cutting edge research findings that explore the complex relationships between genomic instability and inflammation.

8:00 AM – 8:30 AM CDT | Consequences of Genomic Instability During Embryonic Development John Schimenti, Cornell University 8:30 AM – 9:00 AM CDT | cGAS Suppresses Genomic Instability as a Decelerator of Replication Forks Li Lan, MD, PhD, Massachusetts General Hospital Cancer Center 9:00 AM – 9:25 AM CDT | Innate immune sensing at the crossroads of carcinogen-induced inflammation and DNA damage Justin Wilson, University of Arizona Cancer Center 9:25 AM – 9:50 AM CDT | DNA Damage as a Consequence and Driver of inflammation Jennifer Kay, MD, PhD, Department of Biological Engineering, Massachusetts Institute of Technology 9:50 AM – 10:00 AM CDT | Q&A Session

22 Symposium 08 8:00 AM - 10:00 AM CDT | Application of Computational Approaches and Machine Learning to Risk Assessment and Genetic Toxicology Chair: Jeffrey C. Bemis, Co-Chair: TBD, Young Investigator: TBD The application of computational toxicology to product safety testing is increasing at a vary fast pace. Based on the opportunities that these new methodologies are providing for data visualization and interpretation, the EMGS membership will benefit from learning about these approaches now so they can prepare for the eventual adoption that is likely inevitable. This session serves as a complement to the EMGS Bioinformatics Challenge that will be occurring at the virtual meetings. Those who are already working in this area will gain additional knowledge from the peer’s case examples and those new to the field will get an introduction to data science and how it is being used.

8:00 AM – 8:30 AM CDT | Application of New Approach Methodologies (NAMs) to Chemical Risk Assessment and Regulatory Decision-Making Maureen Gwinn, Division Director, Biomolecular and Computational Toxicology Division, Center for Computational Toxicology and Exposure, Office of Research and Development, US Environmental Protection Agency 8:30 AM – 9:00 AM CDT | Trends and Application of Computational Toxicology Approaches Agnes Karmaus, Senior Staff Toxicologist at Integrated Laboratory Systems 9:00 AM – 9:30 AM CDT | Implementation of Machine Learning and Robust Dose-Response Modeling In Genotoxicity Screening Leads to Robust Mode of Action Determination and Potency Ranking of Pharmaceuticals Elias Ozolior, Computational Toxicology in Global Pathology and Investigative Tox, Pfizer, Inc. 9:30 AM – 9:45 AM CDT | Machine Learning and Mechanistic Information from a Multi Endpoint Genotoxicity Screen Amy Wilson, AstraZeneca 9:45 AM – 10:00 AM CDT | Machine Learning Applied to Mouse Genotypes Accurately Classifies Genetic Background: Relevance to Human Genotype Classification For Detection of Environmental Mutagen Exposures Hailie Pavanel, University of Western Ontario

23 Symposium 09 8:00 AM - 10:00 AM CDT | Genomic Alterations and Environmental Factors Involved in Neurodevelopmental/Neuropsychiatric Disorders Chair: Christi Walter, Co-Chair: Noboru Hiroi, Young Investigator: TBD A variety of genome alterations are associated with neuropsychiatric disorders ranging from point mutations, to epigenetic changes, trinucleotide repeat expansions, and CNVs. Environmental factors, such as pesticides and air pollution, and maternal physiological conditions such as diabetes and immune disorders, are also associated with neuropsychiatric/neurodevelopmental disorders. De novo mutations related to the paternal age effect are linked with many of these diseases, such as autism spectrum disorders. This symposium will cover various genomic alterations and environmental factors that appear to impose on many neuropsychiatric/neurodevelopmental disorders and that impinge on environmental mutagenesis and genomics.

8:00 AM – 8:30 AM CDT | Genomic Alterations and Environmental Factors Involved in Neurodevelopmental/Neuropsychiatric Disorders Noboru Hiroi, UT Health San Antonio 8:30 AM – 9:00 AM CDT | Heritable Impacts of General Anesthesia: An Urgent Question for Genetic Toxicology and Autism Research Jill Escher, MA, JD, Escher Fund for Autism 9:00 AM – 9:30 AM CDT | Development of a High Content Imaging Assay for Population Variability in Developmental Neurotoxicity Dahea You, PharmD, PhD, NIEHS 9:30 AM – 10:00 AM CDT | Genome-Wide Analysis of Cadmium-Induced Germline Mutations in Adapted and Nonadapted Genotypes Nathan Keith, Lawrence Berkeley National Laboratory

10:00 AM - 11:00 AM CDT | Break

Keynote 02 11:00 AM - 12:00 PM CDT | The Generation of New Diversity in the Sequence of the Human Genome Kári Stefánsson, M.D., Dr Med, deCODE Genetics, Iceland

12:00 PM - 1:30 PM CDT | Break

24 1:30 PM - 3:30 PM CDT | Platform 1: Gentoxicity, Risk Assessment and Public Health Chair: Barbara L. Parsons, PhD, US Food & Drug Administration, National Center for Toxicological Research 1:30 PM – 1:45 PM CDT | Measuring PIG-A Mutation in Erythrocytes from Healthy Individuals: Effects of Diet and Lifestyle Rachel Lawrence, Swansea University 1:45 PM – 2:00 PM CDT |Assessment of Systemic Genetic Damage in Pediatric Inflammatory Bowel Disease Stephen D. Dertinger, PhD, Litron Laboratories 2:00 PM – 2:15 PM CDT | DNA Damage and Induction of Epithelial Mesenchymal Transition in Oral Epithelial Cells Exposed to Electronic Nicotine Delivery System Aerosols Christa Wright, Georgia State University 2:15 PM – 2:30 CDT | DNA Damage and Gene Expression in Human Gastric and Pancreatic Tissue Organoids Treated with Benzo[a]pyrene Angela Lorena Caipa Garcia, King’s College London 2:30 PM – 2:45 PM CDT | Adapting Next-Generation Genetic Toxicology Endpoints to the Human In Vitro Air-Liquid-Interface Airway Tissue Model Yiying Wang, FDA/NCTR 2:45 PM – 3:00 PM CDT | COSMIC Signatures Robustly Explain Mutational Patterns From the lacZ Gene of Transgenic Rodents Exposed to Environmental Mutagens Matthew Meier, Health Canada 3:00 PM – 3:15 PM CDT | Ultra-Deep Sequencing of Blood and Urine to Test for Exposure to Aristolochic Acids Arnoud Boot, Duke-NUS Medical School 3:15 PM – 3:30 PM CDT | Elucidating the Cellular Mechanism of Doxorubicin Induced Cardiotoxicity in the Nucleus and Mitochondria Meghan E Davis, Massachusetts Institute of Technology

1:30 PM - 3:30 PM CDT | Platform 2: DNA Repair (2A) Chair: Patricia L. Opresko, PhD, University of Pittsburgh. New Investigator Co-Chair: Elise Fouquerel , PhD, Thomas Jefferson University 1:30 PM – 1:45 PM CDT | Precision Targeting of Cancer with CRISPR-induced DSBs Mats Ljungman, PhD, The University of Michigan 1:45 PM – 2:00 PM CDT | Proficient Oxidized Ribonucleotide Insertion by a Double Strand Break Repair Polymerase Joonas Jaemsen, NIEHS/NIH 2:00 PM – 2:15 PM CDT | Flap Endonuclease 1 Cleaves RNAs to Resolve R-loops Through DNA Base Excision Repair Yuan Liu, MD, PhD, Florida International University 2:15 PM – 2:30 PM CDT | Molecular and Structural Characterization of Mutagenic and Non- mutagenic Bypass and Extension of Natural Fapy•dG by a Mammalian DNA Polymerase Benjamin James Ryan, University of Kansas Medical Center

25 2:30 PM – 2:45 PM CDT | FiSHing for OG: Recognition and Repair of OG:A Mispairs by MutY Merve Demir, University of California Davis 2:45 PM – 3:00 PM CDT | Investigating the Role of Repair Enzyme MUTYH in Oxidative Damage at Telomeres Mariarosaria De Rosa, University of Pittsburgh - UPMC Hillman Cancer Center 3:00 PM – 3:15 PM CDT | Apurinic Endonuclease (APE1) Diffusion Along DNA is Altered in the Presence of Mg2+ Ions Andrea Lee, University of Vermont 3:15 PM – 3:30 PM CDT | Cohesin SA1 and SA2 are RNA Binding Proteins That Localize to RNA Containing Regions on DNA Hong Wang, North Carolina State University

3:30 PM - 4:00 PM CDT | Break

EMGS Award 4:00 PM - 5:00 PM CDT | Precision Medicine of Cancer: Environment, Molecular Epidemiology and p53 Curtis Harris, MD, NIH, NCI, CCR

5:00 PM - 7:00 PM CDT | Break

Special Interest Groups 7:00 PM - 8:30 PM CDT | Genomics and Data Science 7:00 PM - 8:30 PM CDT | GRAPH

Tuesday, September 15, 2020 Symposium 10 8:00 AM - 10:00 AM CDT | Non-coding RNA: Shedding Light on the Dark Matter of the Genome Chair: Isabelle Miousse, Co-Chair: TBD, Young Investigator: Sumira Phatak Although less than 1.5% of the human genome codes for proteins, as much as 80% is transcribed. Haphazardly deemed junk DNA for decades, we now refer to these resulting transcripts as non- coding RNA (ncRNA) and know that they serve a number of essential regulatory functions. Circular RNA (circRNA), long non-coding RNA (lncRNA), and microRNA (miRNA) are examples of ncRNA species involved with maintenance of homeostasis, and their roles in environmentally- induced disease are only starting to be discovered. Circular RNA (circRNA) are more abundant in most cells than mRNA and serve as miRNA sponges, transcription factors, and alternative splicing guides, functions that ultimately regulate gene expression. These circRNA are aberrantly expressed in a number of cancers, including colorectal cancer, and may be developed to serve as diagnostic or therapeutic tools in the future. Long non-coding RNA (lncRNA) make up the majority of the non-coding transcriptome in humans, with more than 30,000 identified to date. Although most lncRNA are yet to be characterized, this diverse group

26 of molecules is well known to regulate gene transcription, post-transcriptional modifications, and epigenetic regulation. Evidence suggests that both imprinting and X-chromosome inactivation are lncRNA directed processes. Dysregulation of several lncRNAs has been identified in neurological diseases and cancers. MicroRNA have been shown to be responsive to various environmental factors including methyl-deficient diet, bioactive food molecules, and toxicants such as heavy metals. miRNA may play an important role in pathogenesis of cancers, appearing to function as tumor suppressors or oncogenes. Ultimately, miRNA may even serve as a cancer diagnostic biomarker or therapeutic tool for prevention and treatment. This symposium will discuss the latest discoveries on function of circRNA, lncRNA, and miRNA, with an emphasis on their potential roles as mechanisms of disease pathogenesis and toxicity.

8:00 AM – 8:20 AM CDT | Single Cell Profiling of Total RNA Using Smart-seq-total Alina Isakova, Stanford University 8:20 AM – 8:40 AM CDT | Non coding RNA: Shedding Light on the Dark Matter of the Genome Ahmad Khalil, Case Western Reserve University School of Medicine 8:40 AM – 9:00 AM CDT | Emerging Role of Long non-coding RNAs in Cancer Precision Medicine R. Stephanie Huang, University of Minnesota 9:00 AM – 9:20 AM CDT | At the Intersection of Neurotoxins, microRNAs (miRNAs) and Inflammatory Neurodegenerative Disease Walter Lukiw, Louisiana State University 9:20 AM – 9:32 AM CDT | Identification of piRNA and PIWIL Machinery in Human Somatic Tissues Rachel Morgan, MPH, University of Michigan School of Public Health 9:32 AM – 9:44 AM CDT | Cannabidiol (CBD) Administration Modifies Genome-Wide Hippocampal DNA Methylation In Adult Mice Nicole Wanner, DVM, University of Minnesota Twin Cities 9:45 AM – 10:00 AM CDT | Q&A

27 Symposium 11 8:00 AM - 10:00 AM CDT | Advancements in Mutagenicity Assessment Chairs: Dan Roberts (Charles River Laboratories) and Bob Young (MilliporeSigma). Young Investigator: Shaofei Zhang (MilliporeSigma) Current genetic toxicity testing strategies identify mutagenic properties of novel products via phenotypic changes in a variety of in vitro and in vivo test systems. For example, the gold standard for detecting mutagenesis in vitro is the bacterial reverse mutation assay that uses highly engineered strains of Salmonella and E.coli to detect restoration of essential amino acid synthesis via reversion to wild-type. The biological relevance of in vitro mutagenic events can be further explored in vivo using transgenic rodent models, where phenotypic change in target/reporter genes is used to assess tissue-specific mutagenesis. To date, assessment of mutagenesis has mostly been limited to indirect measurements of phenotypic change due to mutation of reporter genes. For the first time, transformative new sequencing technologies and bioinformatics permit direct measurement of a variety of endpoints including mutant frequency and mutant spectrum directly in genomic DNA. This session aims to explore how these new technologies can be applied to these existing nonclinical assays and new applications as well as how these nonclinical applications translate to human cancer risk.

8:00 AM – 8:30 AM CDT | Determining Mutational Spectra from Existing Genetox Assays: Ames Test Shoji Matsumura, Kao Corporation 8:30 AM – 8:50 AM CDT | Interspecies Comparison of Chemical-induced Mutagenesis by Duplex Sequencing™ Bob Young, Millipore-Sigma 8:50 AM – 9:20 AM CDT | Integration of Next Generation Sequencing Approaches With Transgenic Rodent Mutation Reporter Models to Assess Somatic and Germ Cell Mutagenesis Francesco Marchetti, Health Canada 9:20 AM – 9:40 AM CDT | Error-Corrected Duplex Sequencing; Bioinformatic Challenges Clint Valentine, TwinStrand 9:40 AM – 10:00 AM CDT | SomaticSiMu: A Mutational Signature Simulator for Benchmarking Alignment-Free Machine Learning Classification of Genomic Signatures David Chen, University of Waterloo

28 Symposium 12 8:00 AM - 10:00 AM CDT | New Discoveries on the Role of the Epigenome in the Relationship between the Environment and Human Health Chair: Karen Huen, Co-Chair: Bambarendage Pinithi Perera (Revised) Links between environmental exposures, diet and human health receive increasing attention. Accumulating evidence indicates that associations between environmental exposures and disease can be driven by changes in the epigenome. For example, many environmental factors have been linked to cancer, with much of the research focused on mutations in cancer genomes. However, widespread epigenetic changes have also been reported for many cancers. This symposium will provide links between specific exposures and epigenetic and other molecular changes in disease processes. The goals of this session are to present novel mechanistic relationships between environmental exposures, epigenomics and disease processes, as well as the possibility of manipulating the epigenome to reduce disease risk.

8:00 AM – 8:30 AM CDT | Cancer Chemoprevention Via Dietary Targeting of Epigenetic Pathways Emily Ho, College of Public Health and Human Sciences, Oregon State University 8:30 AM – 9:00 AM CDT | Applying functional genomics and epigenetics to assess differential susceptibility to pesticide exposure: The PON1 Story Karen Huen, University of California Berkeley 9:00 AM – 9:30 AM CDT | Chromatin Structure in the Healthy and Failing Heart Thomas Vondriska, Departments of Anesthesiology, Medicine, and Physiology, David Geffen School of Medicine, UCLA 9:30 AM – 9:45 AM CDT | Prenatal Lead (Pb) Exposure’s Effect on DNA Methylation (5mC) and Hydroxymethylation (5hmC) in Adolescent Whole Blood Christine A Rygiel, University of Michigan 9:45 AM – 10:00 AM CDT | Evaluation of Circulating miRNAs as Biomarkers for Genotoxicity and Carcinogenicity of Aristolochic Acid Page B. McKinzie, PhD, National Center for Toxicological Research

10:00 AM - 11:00 AM CDT | Break

Keynote 03 11:00 AM - 12:00 PM CDT | The Enigma of Viral Noncoding RNAs Joan A. Steitz, PhD, Sterling Professor of Molecular Biophysics and Biochemistry, Yale School of Medicine

12:00 PM - 1:00 PM CDT | Education, Student and New Investigators Affairs (ESNIA) Committee Meeting

12:00 PM - 1:30 PM CDT | Break

1:30 PM - 3:30 PM CDT | Platform 3: Epigenomics Chair: Mitchell Turker, PhD, Oregon Health & Science University 1:30 PM – 1:45 PM CDT | Towards Detection of the Methylation Status in a Prostate Cancer Gene Using Raman Spectroscopy and Machine Learning Ari Forman, Western University 1:45 PM – 2:00 PM CDT | Intergenerational Effects of In Utero Arsenic Exposure on Mouse Physiology Mathia L Colwell, University of Minnesota 2:00 PM – 2:15 PM CDT | Toxicogenomic Analyses of Histone Deacetylase Inhibitors in TK6 Cells Eunnara Cho, Health Canada 2:15 PM – 2:30 PM CDT | Divergent Effects of Methionine Versus Cysteine Restriction In Vitro Isabelle R. Miousse, PhD, University of Arkansas for Medical Sciences

1:30 PM - 3:30 PM CDT | Platform 4: Genomics and Data Science Chair: Brian Chorley, PhD, US Environmental Protection Agency, Research Triangle Park, NC 1:30 PM – 1:35 PM CDT | Introduction to Session Brian Chorley, PhD, US Environmental Protection Agency, Research Triangle Park, NC 1:35 PM – 2:05 PM CDT | Extension of Error-Corrected Duplex Sequencing to Structural Variant Detection Thomas E. Wilson, MD, PhD, University of Michigan Medical School 2:05 PM – 2:35 PM CDT | The Role of Asbestos Exposure in Ovarian Carcinogenesis: An Integrative Molecular Epidemiological Study Jiri Zavadil, PhD, International Agency for Research on Cancer 2:35 PM – 2:50 PM CDT | Alignment-Free Genome Analysis of SARS-CoV-2 Using Machine Learning Gurjit Singh Randhawa, Western University 2:50 PM – 3:05 PM CDT | Associations of Prenatal Metal Mixtures With Mitochondria DNA and Telomere Length in Mothers and Children Anna R Smith, MPH, UC Berkeley School of Public Health 3:05 PM – 3:20 PM CDT | Impact of Copper Oxide Particle Solubility on Lung Epithelial Cell Toxicity: Response Characterization Using Global Transcriptional Analysis Andrew Boyadzhiev, Health Canada 3:20 PM – 3:30 PM CDT | Wrap-up questions

30 1:30 PM - 3:30 PM CDT | Platform 5: DNA Repair (2B) Chair: Patricia L. Opresko, PhD, University of Pittsburgh. New Investigator Co-Chair: Elise Fouquerel , PhD, Thomas Jefferson University 1:30 PM – 1:45 PM CDT | Human RAD54B Promotes Sensitivity to Prolonged Replication Stress Jennifer Mason, Clemson University 1:45 PM – 2:00 PM CDT | The Yeast Shu Complex Functions in Error-Free Bypass of DNA Abasic Sites Braulio Bonilla, University of Pittsburgh 2:00 PM – 2:15 PM CDT | Impact of N-Nitrosodimethylamine on DNA Damage and Gene Expression in Mice with Varied DNA Repair Capacities Norah Auma Owiti, MIT 2:15 PM – 2:30 PM CDT | UVA Light Induced Mutagenesis in Xeroderma Pigmentosum Variant Cells After Whole Exome Sequencing Carlos Frederico Martins Menck, University of São Paulo 2:30 PM – 2:45 PM CDT | UV-Induced Replication Blockage and ATR Activation Are Mediated by 6-4 Photoproducts Masaoki Kawasumi, MD, PhD, University of Washington 2:45 PM – 3:00 PM CDT | Translesion Synthesis Pathway Activation in UVB-Irradiated Human Skin is Impacted by Age and IGF-1 Receptor Status Michael George Kemp, PhD, Wright State University 3:00 PM – 3:15 PM CDT | Exploring the Mechanisms of Cruciform DNA-Induced Genetic Instability in Eukaryotic Systems Pooja P Mandke, University of Texas at Austin 3:15 PM – 3:30 PM CDT | Pervasive Hemin-Induced Genome Toxicity in Experimental Intracerebral Hemorrhage (ICH): Implications and Therapeutic Interventions Joy Mitra, PhD, Houston Methodist Research Institute

3:30 PM - 4:00 PM CDT | Break

4:00 PM – 5:00 PM CDT | Young Scientist Award: Genome Integrity in Parkinson's Disease: From Biomarker to Mechanism Laurie Sanders, Duke University Medical Center

5:00 PM - 7:00 PM CDT | Break

Special Interest Groups 7:00 PM - 8:30 PM CDT | Applied Genetic Toxicology (DOWNLOAD MEETING AGENDA) 7:00 PM - 8:30 PM CDT | Germ Cell and Heritable Effects (DOWNLOAD MEETING AGENDA)

31 Wednesday, September 16, 2020 Symposium 13 8:00 AM - 10:00 AM CDT | The Landscape of Human Germline Mutation (Sponsored by: Elsevier) Chair: Jonatan Axelsson, Co-Chair: Carole Yauk, Young Investigator: Mathia Colwell With increasing amounts of information emerging from global genome sequencing analyses we’re beginning to more deeply understand the processes, landscape and impacts of de novo mutations mediated through the male and female germline. In this session we will hear about the most recent discoveries about the role of de novo mutations as a cause of diseases such as intellectual disability and male infertility, including the impact of non-coding mutations. Speakers will present their findings on baseline rates of de novo mutations, how mutation rates differ between populations and families, and how methylation can influence mutation locations. Finally, we will hear about work exploring the contributions of “gonosomal”, “post-primordial germ cell specification” and “single gamete” mutations to overall mutation burden and impact on the next generation.

8:00 AM – 8:30 AM CDT | Causes and Consequences of de novo Mutations in Health and Disease Joris Veltman, Newcastle University, UK 8:30 AM – 9:00 AM CDT | Germline Mutation Rates in Young Adults Predict Longevity and Reproductive Lifespan Aaron Quinlan, University of Utah 9:00 AM – 9:30 AM CDT | Germline Mutation variation in Mice and Humans Sarah Lindsay, Wellcome Sanger Institute 9:30 AM – 9:45 AM CDT | Development of a High-Throughput Assay System to Study Recurrent Copy Number Variation in Saccharomyces cerevisiae Ruthie Watson, Colorado State University 9:45 AM – 10:00 AM CDT | Heterozygosity: A Meiosis-linked Intrinsic Mutagen in Mice Nicholas Alexander Boehler, Western University, Canada

32 Symposium 14 8:00 AM - 10:00 AM CDT | Dynamic RNA Modifications: Roles in Environmental Response and Disease Chair: Frederick L. Tyson, Co-Chair: Daniel Shaughnessy, Young Investigator: Jaclyn Goodrich Dynamic RNA modifications can interpret environmental challenges and respond by altering gene regulation, biological pathways and disease outcomes. Emerging evidence suggests that chemical modifications to RNA have critical roles in basic biological processes including embryonic stem cell differentiation, excitotoxic cell death, development, intergenerational inheritance of acquired traits, regulation of RNA stability, temperature adaptation, meiotic progression, alternative splicing, gene expression and regulation of RNA-RNA and RNA-protein binding interactions. The impact of the environment on chemical modifications of RNA molecules (the epitranscriptome) in the development of adverse human health outcomes is relatively unexplored. Technology advances in recent years have accelerated the detection of RNA modifications, and the RNA Modification Database currently lists approximately 100 RNA modifications identified in eukaryotic cells. This database also reveals transfer and ribosomal RNA are heavily modified, and many of these same modifications occur in messenger RNA and non-coding RNAs. The function of most of these modifications remains a mystery, despite their potential to influence RNA properties and functions and interactions with other molecules. This session will bring together leading investigators in the emerging area of functional RNA modifications to discuss the state of the science including how environmental exposures impact this layer of cellular regulation.

8:00 AM – 8:25 AM CDT | Interactions Between mRNA Methylation and Histone Modifications Crystal Zhao, Sanford Burnham Prebys Medical Discovery Institute 8:25 AM – 8:50 AM CDT | Tracking RNA Modifications to Understand Early Health Effects of Oxidative-Prone Air Lydia Contreras, University of Texas at Austin 8:50 AM – 9:15 AM CDT | Epitranscriptomic Marks Translationally Regulate Stress Response Programs to Protect Against Environmental Insults Thomas J. Begley, The State University of New York at Albany 9:15 AM – 9:35 AM CDT | Epigenetic Inheritance of Acquired Traits Through Sperm RNAs and Modifications Qi Chen, University of California, Riverside 9:35 AM – 10:00 AM CDT | The Epitranscriptome at the Crossroads of Diet and Environmental Exposure in Liver Diseases Juliane Beier, University of Pittsburgh

33 Symposium 15 8:00 AM - 10:00 AM CDT | Alternative DNA Structures: Naturally Occurring Impediments to Transcription and Replication, and Sources of Genomic Instability Chair: Karen Vasquez, Co-Chair: Kristin Eckert, Young Investigator: Guliang Wang Repetitive sequences are abundant in the human genome, and have the capacity to adopt alternative (i.e. non-B) DNA structures. More than a dozen non-B DNA structures have been characterized to date, e.g., Z-DNA, H-DNA, G4-DNA, hairpins, and cruciforms. These alternative DNA structure-forming sequences have been found to impact DNA metabolic processes, as they can act as impediments to DNA and RNA polymerases, helicases, DNA repair enzymes, etc. Moreover, these structure-forming sequences have been identified as a naturally occurring source of genetic instability, and have been implicated in disease etiology and evolution. For example, non-B DNA-forming sequences have been found to be substantially enriched at translocation breakpoints in human cancer genomes. Because certain sequence elements are required for the formation of a particular non-B DNA structure (e.g. inverted repeats can adopt cruciform structures, alternating purine-pyrimidine dinucleotide repeats can adopt Z-DNA structures, etc.), algorithms have been designed to search databases for potential non-B DNA- forming sequences. Such information can inform on their potential roles in modulating transcription, replication, repair, and disease etiology. In this session, a new technology for genome-wide mapping of non-B DNA structures will be presented. In addition, their roles in disease etiology, and the mechanisms involved in their error-generating and error-free processing will be discussed.

8:00 AM – 8:30 AM CDT | RNA Export and Processing Act Cooperatively to Prevent RNA Polymerase Stalling and Fragility of CAG Repeats Catherine Freudenreich, Tufts University 8:30 AM – 9:00 AM CDT | Alternative DNA Structures: Naturally Occurring Impediments to Transcription and Replication, and Sources of Genomic Instability Andres Aguilera, Universidad de Sevilla 9:00 AM – 9:15 AM CDT | Distinct Mechanisms of DNA Structure-Induced Genetic Instability Guliang Wang, University of Texas at Austin 9:15 AM – 9:30 AM CDT | G-Quadruplex Sequences are Barriers to Replicative DNA Polymerases and Hotspots of Mutagenesis Kristin A. Eckert, PhD, Pennsylvania State University College of Medicine 9:30 AM – 10:00 AM CDT | Alternative DNA Structures and Genomic Instability David L. Levens, Laboratory of Pathology, National Cancer Institute

10:00 AM - 11:00 AM CDT | Break

Committee Meetings 11:00 AM - 12:00 PM CDT | Awards and Honors Committee 11:00 AM - 12:00 PM CDT | Membership/PD Committee 11:00 AM - 12:00 PM CDT | Finance/Fundraising Committee 11:00 AM - 12:00 PM CDT | Hollaender Outreach Committee

12:00 PM - 1:30 PM CDT | Break

12:00 PM - 1:30 PM CDT | EMGS Business Meeting

1:30 PM - 3:30 PM CDT | Platform 6: Applied Genetic Toxicology Chair: Rosalie K. Elespuru, PhD, US Food & Drug Administration 1:30 PM – 1:45 PM CDT | TubulinTracker, a Novel In Vitro Reporter Assay to Study Intracellular Microtubule Dynamics, Cell Cycle Progression and Aneugenicity Giel Hendriks, PhD, Toxys 1:45 PM – 2:00 PM CDT | Quantitative Comparison of DNA Damage Responses of Three Human Hepatocytes for in Vitro Genotoxicity Assessment Xiaoqing Guo, NCTR, US FDA 2:00 PM – 2:15 PM CDT | Analysis of Genetic Susceptibility Factors for N-nitrosamine-Induced Toxicity, Genomic Instability, and Cancer Jennifer Kay, Massachusetts Institute of Technology 2:15 PM – 2:30 PM CDT | Mutagenicity of a New Phenylbenzotriazol, the Non-Chlorinated Pbta-9, in Bacteria and Mice Gisela de Aragão Umbuzeiro, UNICAMP 2:30 PM – 2:45 PM CDT | Unexpected 3-Butene-1,2-Diol-Mediated Genotoxicity Implies Alternative Mechanism of 1,3-Butadiene Carcinogenesis Jun Nakamura, Osaka Prefecture University 2:45 PM – 3:00 PM CDT | Application of Multiple in Vitro Genetic Toxicology Endpoints Enables Comprehensive Characterization of Test Compounds’ Mode/Mechanism of Action Steven M. Bryce, Litron Laboratories 3:00 PM – 3:15 PM CDT | Innovative Approach To Predict DNA Adduct Formation of Environmental Contaminants Conan Maël, University of Rennes 3:15 PM – 3:30 PM CDT | Developing in Vitro Alternatives to In Vivo Genetic Toxicology Tests: HepaRG™ CometChip as an In Vitro Alternative to the Comet Assay Leslie Recio, PhD, DABT, Integrated Laboratory Systems, Inc.

1:30 PM - 3:30 PM CDT | Platform 7: Detecting Rare Mutations, Gene and Germ Cell Toxicity Chair: Jonatan Axelsson, MD, PhD, Lund University and Skåne University Hospital | Co- chair: Abigail Bline, UCLA Fielding School of Public Health 1:30 PM – 1:45 PM CDT | PFASs Impair Meiotic Progression and Alter Ribonucleoprotein Granule Dynamics in C. elegans Abigail Bline, University of California Los Angeles

35 1:45 PM – 2:00 PM CDT | Comparison of Duplex Sequencing and CarcSeq Error-Corrected NGS methods for Detection of Preneoplastic Clonal Expansions in Normal, Healthy Human Tissues Meagan Myers, PhD, DHHS/FDA/NCTR 2:00 PM – 2:15 PM CDT | Microsatellite Mutation Frequencies in River Otters (Lontra canadensis) from the Athabasca Oil Sands Region are Correlated to Polycyclic Aromatic Compound Tissue Burden Helina Gyasi, University of Ottawa 2:15 PM – 2:30 PM CDT | Adopting Duplex Sequencing Technology for Genetic Toxicity Testing: A Proof-of-Concept Mutagenesis Experiment with N-Ethyl-N-Nitrosourea (ENU)- Exposed Rats Stephanie Smith-Roe, National Toxicology Program 2:30 PM – 2:45 PM CDT | Indigenous Voices Needed: EMGS Calls for Diversity in Microplastic Genotoxicity Research Amanda Louise Morin, University of Western Ontario

1:30 PM - 3:30 PM CDT | EMGS Bioinformatics Challenge Chair: Maxwell C. K. Leung, Ph.D., Arizona State University, Jeffrey Bemis, Ph.D., Litron Laboratories 1:35 PM – 1:50 PM CDT | SomaticSim: A Somatic Mutational Signature Simulator for Benchmarking Alignment-free Machine Learning Analyses David Chen, Western University 1:50 PM – 2:05 PM CDT | Integrating Knowledge of Carcinogenicity Adverse Outcome Pathways (AOPs) with Experimental Data Steven Kane, Lhasa limited 2:05 PM – 2:20 PM CDT | Weighted Coregulation Network Analysis to Identify Genotoxic and Cardiotoxic Potential of Chemotherapy Jui-Hua Hsieh, National Institute of Environmental Health Sciences 2:20 PM – 2:35 PM CDT | Machine Learning-based Analysis of Genome Sequences for Studying the Impact of Extreme Environments on Genomic Signatures Maximillian Soltysiak, Western University 2:35 PM – 2:50 PM CDT | Genotoxicity Data Visualization Tool for Fragrance Materials Houlger Moustakas, Research Institute for Fragrance Materials, Inc.

3:30 PM - 4:00 PM CDT | Break

Committee Meetings 4:00 PM - 5:00 PM CDT | Publication Policy Committee 4:00 PM - 5:00 PM CDT | Program Committee 4:00 PM - 5:00 PM CDT | Public Relations & Communications Committee 4:00 PM - 5:00 PM CDT | SIG Chairs Committee

5:00 PM - 7:00 PM CDT | Break

Panel 7:00 PM – 8:30 PM CDT | EMGS Mentor/Mentee Panel, Sponsored by the WEMGS and SNI: "How do I get there? Career Panels to Guide Students, Postdocs, and New Investigators in Their Next Career Move"

Thursday, September 24, 2020 2:00 PM - 5:00 PM CDT | EMGS Council Meeting

37 Posters

Applied Genetic Toxicology P8 Dihydronicotinamide Riboside Promotes Cell-Specific P1 Cytotoxicity by Tipping the Balance Between Oxidative POSTER CANCELLED: Classification of In Vitro Stress and Metabolic Regulation. Sonavane M, Hayat F, Genotoxicants Requiring Metabolic Activation using a Makarov M, Migaud ME, Gassman NR. University of South Multiplexed Assay (MultiFlowTM). Smith R. Covance Alabama College of Medicine and Mitchell Cancer Institute, Laboratories, Harrogate, United Kingdom. Mobile, AL, United States.

P2 P9 A Novel Genome Sequence-based Mutagenicity Assay Dihydroxyacetone Exposure Induces Metabolic Imbalance using Salmonella Typhimurium TA100 Model Improve Induces Mitochondrial Dysfunction. Mehta R, Smith K, Mutagenicity and Carcinogenicity Evaluation of Chemicals. Seiger J, Sonavane M, Gassman NR. University of South Otsubo Y, Matsumura S, Ikeda N, Morita O. R&D, Safety Alabama College of Medicine and Mitchell Cancer Institute, Science Research, Kao Corporation, Tokyo, Japan. Mobile, AL, United States.

P3 P10 Adopting Duplex Sequencing Technology for Genetic Establishing A Hepatic Culture Model To Assess Altered Toxicity Testing: A Proof-of-Concept Mutagenesis Chemical Susceptibility Due To Pre-existing Liver Steatosis. Tucker NN1, Nelson GM2, Harrill JA2, Chorley BN2. Experiment with N-Ethyl-N-Nitrosourea (ENU)-Exposed 1 1 2 2 2 Oak Ridge Institute for Science Education, Oak Ridge, TN, Rats. Smith-Roe SL , Hobbs CA , Recio L , Streicker MA , 2 Rivas MV2, Pratt GA3, Higgins JE3, Schmidt EK3, Valentine CC3, United States US EPA Center for Computational Toxicology Williams LN3, Salk JJ3, Witt KL1. 1National Toxicology Program, and Exposure, Durham NC, United States. NIEHS, Research Triangle Park, NC, United States, 2Integrated Laboratory Systems, Inc., Research Triangle Park, NC, United P11 States, 3TwinStrand Biosciences, Inc., Seattle, WA, United Flow Cytometric Evaluation of Genotoxicity Pathways Induced by Non-Covalent DNA-Binders. Roberts DJ2, States. 1 2 2 2 Hendriks G , Miller, M , Soomer-James, J , Kaye, S , Stankowski Jr., LF2 and Welss, M2. 1Toxys BV, Leiden, P4 2 Analysis Of The Cytotoxicity And Genotoxicity of Digested Netherlands, Charles River Laboratories, Skokie, IL, United Titanium Dioxide Nanomaterials In Intestinal Cells. Louro States. H1,2, Vieira A1, Gramacho AC1, Rolo D1, Vital N1, Martins C1,3, Assunção R1,3, Alvito P1,3, Gonçalves L4, Bettencourt AF4, Silva P12 MJ1,2. 1National Institute of Health Dr. Ricardo Jorge (INSA), Genotoxicity Assessment Using In Vitro Micronucleus Lisbon, Portugal, 2ToxOmics – Centre for Toxicogenomics and Assay and a High-Throughput Transcriptomic Biomarker, TGx-DDI. Xie H1, Kulkarni R2, Li HH3, Chen R3, Fornace Jr. AJ3 Human Health, NOVA Medical School, NOVA University, 1 3 MilliporeSigma, BioReliance® Toxicology Testing Services, Lisbon, Portugal, CESAM - Centre for Environmental and 2 Marine Studies, University of Aveiro, Aveiro, Portugal, Rockville, Maryland, United States, EMD Serono Research & 4 Development Institute, Inc. Billerica, MA, United States, Research Institute for Medicines (iMed.ULisboa), Faculty of 3 Pharmacy, Universidade de Lisboa, Portugal. Georgetown University Medical Center, Washington, DC, United States. P5 Concentration- and Time-Dependent Metabolic Activation P13 and Genotoxicity of Benzo[a]pyrene in LMH 3D Spheroid. HepaRG™CometChip and HepaRG™ Micronucleus Assay Sharin T1,2, Gyasi H1,2, Jones SP1, Browning J1, Crump D1, as Non-Animal Alternatives to Follow Up Positive In Vitro O’Brien JM1. 1National Wildlife Research Centre, Environment Genotoxicity Findings: Case Study with Naphthalene. Recio and Climate Change Canada, Ottawa, Ontario, Canada, L, Christy N, Fowler J, Swartz C, Martin L, Sly J, Burke K. 2University of Ottawa, Ottawa, Ontario, Canada. Integrated Laboratory Systems, Inc., Research Triangle Park, NC, United States P6 Detection of DNA Damage in Sea Urchin Coelomocytes P14 With Fast Micromethod. Liu F1, Last K1, Henry T2, Reinardy HESI Drug Case Study: An Integrated Approach to 1,3 1 Pharmaceutical Safety Testing Using High-Throughput H . The Scottish Association for Marine Science, Oban, 1 2 CometChip and TGx-DDI Analyses. Buick JK , Rowan-Carroll United Kingdom, Heriot-Watt University, Edinburgh, United 1 1 1 2,3 2,3 2,3 Kingdom, 3The University Centre in Svalbard, Longyearbyen, A , Gagné R , Williams A , Chen R , Li HH , Fornace Jr. AJ , Chao C4, Engelward BP4, Frötschl R5, Ellinger-Zeigelbauer H6, Norway. 7 8 1 1 Pettit SD , Aubrecht J , Yauk CL . Environmental Health Science and Research Bureau, Health Canada, Ottawa, P7 2 Determining Genotoxic Potential and Mode of Action in a Ontario, Canada, Lombardi Comprehensive Cancer Center, Single Mammalian Cell Assay. Roberts DJ, Odia O, Moy M, Georgetown University Medical Center, Washington, District of Columbia, United States, 3Georgetown University, Washington Hurtado S, Wells M, Stankowski Jr. LF. Charles River 4 Laboratories, Skokie, IL, United States. District of Columbia, United States, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States, 5 Federal Institute for Drugs and Medical Devices (BfArM), Bonn, Germany, 6Bayer AG, Pharmaceuticals, Translational Sciences, 38

Wuppertal, Germany, 7Health and Environmental Sciences P22 Institute, Washington, DC, United States, 8Takeda APE1 Cleavage of Abasic Ribonucleotides Embedded in Pharmaceuticals USA Inc., Cambridge, Massachusetts, United DNA. Hoitsma NM, Freudenthal BD. University of Kansas States. Medical Center, Kansas City, KS, United States.

P15 P23 In vitro Mechanism of Oxidative Stress and DNA Damage Autophagy Contributes To Xeroderma Pigmentosum Induction by Insulin. Kodandaraman G, Bankoglu EE, Stopper Variant Cell Survival After UVA Light-Induced Oxidative H. University of Wuerzburg, Wuerzburg, Germany. Stress. Moreno NC, Gomes LR, Menck CFM, Souza-Pinto NC. University of São Paulo Institute of Chemistry, São Paulo, SP, P16 Brazil. Particle Size and Solubility Influence the Uptake and Genotoxicity of Nickel Compounds in Bronchial Epithelial P24 BEAS-2B cells – toxic Effect of Nickel Oxide Nanoparticles Carbon Metabolism Impacts the Efficiency of Double- Halted by Cytochalasin B. Siivola KM, Suhonen S, Norppa H. Strand Break Resection in Preparation for Homologous Finnish Institute of Occupational Health, Helsinki, Finland. Recombination. Lomonaco S, Wilson TE. University of Michigan Medical School, Ann Arbor, MI, United States. P17 Potency of per- and polyfluoroalkyl Substances (PFAS) in P25 Exposed Human Liver Cell Spheroids Using High- Characterizing Drosophila Mutagen Sensitive Alleles throughput Transcriptomics. Reardon AJF1, Rowan-Carroll Through a Collaborative Course-based Undergraduate A1, Ferguson SS2, Gagne R1, Kuo B1, Leingartner K1, Williams Research Experience (CURE). Bolterstein EB1, Kohl KP2, A1, Nong A1, Lorusso L3, Levicki C3, Bourdon-Lacombe JA4, Stoffregen EP3, and Swanson CI4. 1Northestern Illinois Carrier R4 Moffat I4, Yauk CL1, Atlas E1. 1Environmental Health University, Chicago, IL, United States, 2Winthrop University, Science & Research Bureau, Health Canada, Ottawa, Ontario, Rock Hill, SC, United States, 3Lewis-Clark State College, Canada, 2National Toxicology Program, NIEHS, Durham, NC, Lewiston, ID, United States, 4Arcadia University, Glenside, PA, United States 3Chemicals and Environmental Health United States. Management Bureau, Health Canada, Ottawa, Ontario, Canada, 4Water and Air Quality Bureau, Health Canada, P26 Ottawa, Ontario, Canada. CometChip Analysis of Human Primary Lymphocytes Enables Quantification of Inter-Individual Differences in the P18 Kinetics of Oxidative Damage Repair. Kaushal S, Ngo LP, Quantitative Concentration-response Analysis Using the in Chaim IA, Mazzucato P, Ricciardi C, Nagel ZD, Samson LD, vitro Micronucleus Test to Inform Genotoxicity Evaluation Engelward BP. Massachusetts Institute of Technology, for Risk Assessment of 292 Chemicals. Kuo B1, Beal MA2, Cambridge, MA, United States. Wills J1, White PA1, Marchetti F1, Nong A1, Barton-Maclaren T2, Houck K3, Yauk CL1. 1Environmental Health Science and P27 Research Bureau, Health Canada, Ottawa, Ontario, Canada, CRISPR/Cas9 Causes R-loops. Dzikowski, MM, Hanakahi, LA. 2Existing Substances and Risk Assessment Bureau, Health University of Illinois at Chicago, Rockford, IL, United States. Canada, Ottawa, Ontario, Canada, 3National Center for Computational Toxicology, US EPA, Research Triangle Park, P28 NC, United States. DNA Single-strand Break Repair and Signaling by APE1. Yan S, Lin Y, Raj J, Li J, Ha A, Hossain MA, Richardson C, P19 Mukherjee P. University of North Carolinat at Charlotte, S-Allylcysteine Induces Cytotoxicity and Tumoral Growth Charlotte, NC, United States. Reduction in 3D-Multicellular Spheroids Derived from HepG2 cells. Leão TK1, Machado ART1, Rodrigues KT2, Ribeiro P29 DL2, Antunes LMG1. 1University of São Paulo, Ribeirão Preto, Droplet Digital PCR-Based Detection Of Mutant mRNAs SP, Brazil, 2Ribeirão Preto Medical School, University of São Conferring Ciprofloxacin Resistance In P. aeruginosa. Paulo, Ribeirão Preto, SP, Brazil. Garcia-Villada L, Degtyareva NP, Doetsch PW. NIEHS, Durham, NC, United States. P20 Transgenerational Effect of N-nitrosodimethylamine in P30 Three Generations of Drosophila Melanogaster. Evangelista Excision of Oxidatively Damaged Bases in G-quadruplexes R1, Ramos-Morales P. Laboratorio de Genética y Toxicología by the DNA Glycosylases NEIL1 and mNeil3. Lotsof ER1, Ambiental – Banco de Moscas, UNAM, Coyoacan, Mexico. Conlon SG1, Anderson-Steele BM1, Bumgarner JD1, Mifflin K1, Fleming AA2, Burrows CJ2, David SS1. 1University of California, Davis, Davis, CA, United States, 2University of Utah, Salt Lake DNA Repair & Mutagenic Mechanisms City, UT, United States.

P21 P31 Nucleotide Excision Repair Hotspots and Coldspots of UV Faithful Ligation By DNA Ligase 1 And Its Role In Damage in Humans. Li W, Jiang Y, Lindsey-Boltz LA, Yang Y, Maintaining Genome Integrity. Arana ME, Williams JS, Li Y, Sancar A. University of North Carolina at Chapel Hill, Tumbale PP, Rana JA, Williams RS, Kunkel TA. NIEHS, Chapel Hill, NC, United States. Research Triangle Park, NC, United States.

P32 N1, Manjanatha MG1. 1National Center for Toxicological POSTER CANCELLED: Flap Endonuclease 1 Cleaves RNAs Research, Jefferson, AR, United States. 2National Toxicology to Resolve R-loops Through DNA Base Excision Repair. Liu Program, NIEHS, Research Triangle Park, Durham, NC, United Y1, Laverde EE1, Tsegay P1, Shaver M1, Hutcheson JD1, and States. Balakrishnan L2. 1Florida International University, Miami, FL, United States, 2Indiana-Purdue University, Indianapolis, IN, P42 United States. Metabolic Changes Caused by Endogenous Redox Stress Mirror Alterations Triggered by Exposure to Exogenous P33 Oxidative Agents. Placentra VC, Degtyareva NP, Mueller GA, POSTER CANCELLED: Genoprotective Effects of Chaga Doetsch PD. NIEHS, Durham, NC, United States. Mushroom (Inonotus obliquus) Polysaccharides in UVB- exposed Embryonic Zebrafish (Danio rerio) Through P43 Coordinated Expression of DNA Repair Genes. Ibrahim J1, Novel Roles for the RAD51 Paralogs in DNA Strand Specific Mohanty S2, Das B2. 1Cairo University, Cairo, Egypt, 2KIIT Damage Tolerance. Bernstein KA1, Bonilla B1, Mertz TM2, University, Bhubaneswar, India. Rapchak KS1, Zaher HS3, Mosammaparast N3, Roberts SA2. 1University of Pittsburgh, Pittsburgh, PA, United States, P34 2Washington State University, Pullman, WA, United States, Genotoxicity of Omega-3 Fatty Acids from the Ames Test 3Washington University, St. Louis, MO, United States. Viewpoint. Grúz P1, Shimizu M2, Yamada M3, Sugiyama K1, Honma M1. 1National Institute of Health Sciences, Kawasaki, P44 Japan, 2Tokyo Healthcare University, Tokyo, Japan, 3National Oxidative Damage Attenuates Non-B DNA-induced Genetic Defense Academy, Yokosuka, Japan. Instability. Zewail-Foote M1, del Mundo IMA2, Vasquez KM2. 1Southwestern University, Georgetown, TX, United States, P35 2University of Texas at Austin, Dell Pediatric Research Institute, Glycidamide-induced Hypermutation in Single Stranded Austin, TX, United States. DNA. Hudson K, Saini N, Sterling JF, Klimczak LJ, Gordenin DA. NIEHS, Durham, NC, United States. P45 Oxidative DNA Damage in Parkinson’s Disease-Associated P36 Genes. Marin C, Lai Y, Vazquez E, Fan RZ, Tieu K, Liu Y. High Resolution in vivo Mapping of Yeast Mitotic DSB Florida International University, Miami, FL, United States. Resection Supports a Combined Endo- and Exonucleolytic Mechanism. Bazzano DJ, Wilson TE. University of Michigan, P46 Ann Arbor, MI, United States. Probing the Functional Impact of Cancer-Associated Variations within the Interdomain Connector of the Adenine P37 Glycosylase MUTYH. Khuu C, David SS. University of High-throughput Screening for Resistance to Colon California Davis, Davis, CA, United States. Cancer-associated Carcinogen in Budding Yeast Identifies both DNA Repair and Ribosomal Protein Genes. Fasullo M, P47 Dolan M, St. John N, Zaidi F. SUNY Polytechnic Institute, Regulation of Budding Yeast Hrq1 Helicase During DNA Albany, NY, United States. Crosslink Repair. Luong TT, Bohm S, Bernstein KA. University of Pittsburgh Cancer Institute, Pittsburgh, PA, United States. P38 Impact Of G-quadruplexes On DNA Double-Strand Break P48 Repair By End Joining. Selvaraj S, Seiver JA, Hanakahi LA. POSTER CANCELLED: Single Molecule Visualization of University of Illinois at Chicago, Chicago, IL, United States. Base Excision Repair Coordination. Fairlamb MS1, Bain, FE2, Boehm, EM2, Spies, M2, Washington, MT2, Tomkinson, AE3, P39 Freudenthal, BD1. 1University of Kansas Medical Center, Insights from Disease and Tumor-related Mutations in TFIIH Kansas City, KS, United States, 2Carver College of Medicine and XPG. Tsutakawa SE1, Yan, C2, Sarker, AH1, Arvai, AS3, University of Iowa, Iowa City, IA United States, 3University of Dodd, T2, He, Y4, Lichtarge, O5, Griffith, JD6, Cooper, PK1, New Mexico, Albuquerque, NM, United States. Ivanov, I2, and Tainer JA1. 1Lawrence Berkeley National Laboratory, Berkeley, CA, United States, 2Georgia State, Atlanta, GA, United States, 3The Scripps Research Institute, La P49 Jolla, CA, United States, 4Northwestern, Evanston, IL, United Structural and Evolutionary Aspects of the Eukaryotic NER States, 5Baylor School of Medicine, Houston, TX, United States, Pathway. Feltrin RS1, Segatto ALA2, Souza TA3, Schuch AP1. 6UNC Chapel Hill, NC, United States, 7MD Anderson, Houston, 1Federal University of Santa Maria, Santa Maria, RS, Brazil, TX, United States. 2Federal Institute of Rio Grande do Sul, Caxias do Sul, RS, Brazil. 3TauGC Bioinformatics, Sao Paulo, SP, Brazil. P40 Mechanisms of Telomerase Inhibition by Oxidized and P50 Therapeutic dNTPs. Sanford S1, Welfer G2, Freudenthal BD2, POSTER CANCELLED: UV-Exposure And Endogenous Opresko PL1. 1University of Pittsburgh, Pittsburgh, PA, United DNA Damage Cause Genome Changes In Skin Of Healthy States, 2Kansas University Medical Center, Kansas City, KS, Humans Through Synthesis Across Lesions And Error United States. Prone Double-Strand Break Repair. Saini N, Giacobone C, Pappas B, Klimczak LJ, Li J-L, Fargo D, Bai R, Gerrish K, Innes P41 C, Schurman S, Gordenin DA. NIEHS, Durham, NC, United Mechanistic Genotoxicity Assessment of Black Cohosh States. Extract in TK6 cells. Seo J-E1, Guo X1, Petibone DM1, Shelton S1, Chen Y1, Li X1, Tryndyak V1, Smith-Roe SL2, Witt KL2, Mei

P51 P59 Telomeric 8oxoG Damage Induces Cellular Senescence by The Role of Error-Prone polymerases on Tumor Cell Disrupting Telomere Replication. Barnes RP1, De Rosa M1, Resistance to Temozolomide. Latancia M , Bastos AU, Thosar S1, Bruchez M2, Opresko PL1. 1University of Pittsburgh Moreno NC, Jardim D, Rocha CRR, Menck CFM. Institute of and UPMC Hillman Cancer Center, Pittsburgh, PA, United Biomedical Sciences, University of Sao Paulo (USP), Sao States, 2Carnegie Mellon University, PA, United States. Paulo, SP, Brazil.

P52 P60 POSTER CANCELLED: Temozolomide Attenuates Frataxin The Role of STEAP2 on Aggressive Prostate Cancer Traits Deficiency Through Contractions of GAA Repeats Via Base and Androgen Responses. Jones L1,2, Lee H-J2, Wang Y-L2, Excision Repair. Lai Y, Liu Y. Florida International University, Kang K2, Debasish B2, He B2, Nguyen-Chi A1, Burnell S1,4 Pan Miami, FL, United States. P-Y2, Chen S-H2, Jenkins GJ1, Doak S1. 1Swansea University, Swansea, Wales, United Kingdom, 2Houston Methodist P53 Research Institute, Houston, Texas, United States, 3Cardiff The Aging Immune System Drives Senescence and University, Cardiff, Wales, United Kingdom. Dysfunction of Peripheral Tissues. Yousefzadeh MJ1,2, Flores RR1,2, Cui Y3, Brooks RW1, Zhao J1, Angelini LA1,2, Wade P61 EA1, Trussoni CE4, Kato JI1, McGuckian CA1,2, Wang D4, Dong TIM-3 Polymorphism Regulates Gene Expression and is Q4, Patil P4, Li K4, Lu A5,6, O’Kelly RD1,2, Ladiges WL7, Burd CE8, Associated with the Susceptibility and Clinical Prognosis of LaRusso NF4, Pillai SPS9, Vo NV4, Kelley EE10, Wang Y3, Huard Esophageal Squamous Cell Carcinoma. Wang N, Cui S-J, Li J5,6, Robbins PD1,2, Niedernhofer LJ1,2. 1University of Minnesota, Y, Zhou R-M, Liu L, Cao S-R, Huang X, Huo X-R. The Fourth Minneapolis, MN, United States, 2The Scripps Research Hospital of Hebei Medical University, Cancer Institute of Hebei Institute, San Diego, CA, United States, 3University of California Province, Shijiazhuang, Hebei, PR China Riverside, Riverside, CA, United States, 4University of Pittsburgh, Pittsburgh, PA, United States, 5Steadman Philippon P62 Research Institute, Vail, CO, United States, 6University of Texas Topoisomerase 1 Mutants and G-quadruplex-induced Health Science Center at Houston, Houston, TX, United States, Genomic Instability. Berroyer A, Kim N. University of Texas 7University of Washington, Seattle, WA, United States, 8The Health Science Center, Houston, TX, United States. Ohio State University, Columbus, OH, United States, 9Fred Hutchinson Cancer Research Center, Seattle, WA, United P63 States, 10West Virginia University, Morgantown, WV, United Uncovering How RAD51C Mutations Contribute to Cancer States. Predisposition and Sensitize to Chemotherapeutics. Sullivan MR1, Prakash R2, Rawal Y3, Grundy MK1, Baird JM1, P54 Mihalevic MJ1, Rein H1, Garcin EB4, Wang R2, Modesti M4, Sung The BRCA2-interacting Protein, PDS5B, and SPIDR are P3, Swisher EM5, Jasin M2, Bernstein KA1. 1University of Novel Shu Complex Components. Hengel SR, Martino J, Pittsburgh, School of Medicine, Pittsburgh, PA, United States, Brunette G, Barroso-Gonzales J, Moiseeva T, Smith C, 2Memorial Sloan Kettering Cancer Center, New York, NY, Bakkenist C, O'Sullivan R, Bernstein KA. University of United States, 3University of Texas San Antonio Long School of Pittsburgh, Pittsburgh, PA, United States. Medicine, San Antonio, TX, United States, 4Cancer Research Center of Marseille, CNRS, Inserm, Institut Paoli-Calmettes, P55 Aix-Marseille Université, Marseille, France, 5University of The Central DNA Binding Domain of XRCC1 Stimulates the Washington School of Medicine, Seattle, WA, United States. Repair Activity of the DNA Glycosylase NTHL1. Maher RL, Averill AM, Pederson DS. University of Vermont, Burlington, VT, United States. Epigenomics

P56 P64 The Effects of Metabolic Stress on Werner Protein-deficient A Strategy to Identify Compound-induced DNA Methylation Drosophila. Bolterstein EB, Schirmer AE, Epiney DG, Changes and Potential Association to Genotoxicity. Beck Milutinovic R, Salameh C, Uddin S. Northeastern Illinois RSS1, Wilson A2, Doherty A2, Walmsley R3, 1King's College University, Chicago, IL, United States. London, London, United Kingdom, 2AstraZeneca, Cambridge, United Kingsom, 3University of Manchester, Manchester, United P57 Kingsom. The N-terminus of FBH1 is Critical for Anti-recombinase Activity in Human Cells. Hawks AL, Brown P, Wu P, Conway P65 K, Goodson S, Mason JM. Clemson University, Clemson, SC, Development and Validation of a Transcriptomic Biomarker United States. of Chemical-Induced Histone Deacetylase Inhibition in TK6 Cells. Cho E1,2, Rowan-Carroll A1, Williams A1, Corton JC3, Li P58 HH4, Fornace Jr. AJ4, Hobbs CA5, Yauk CL1,2. 1Environmental The NEIL1 DNA Glycosylase Interacts with Mitochondrial Health Science and Research Bureau, Health Canada, Ottawa, Transcription Factor A in Presence of DNA via its N-terminal 2 3 1 1 1 2 ON, Canada, Carleton University, Ottawa, ON, Canada, US Domain. Sharma N , Arrington JF , Thompson M , Terry MD , EPA, Research Triangle Park, NC, United States, 4Lombardi Prakash A1, 1University of South Alabama, Mitchell Cancer 2 Comprehensive Cancer Center, Georgetown University Medical Institute, Mobile, AL, United States, Springhill College, Mobile, Center, Washington, DC, United States, 5Integrated Laboratory AL, United States. Systems Inc., Research Triangle Park, NC, United States.

Cilia C, Dure JE, Shupp MA, Urdapilleta NG, Segovia JA. P66 Universidad Católica Nuestra Señora de la Asunción. Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 P74 Days Exposure to MWCNT-7 or Asbestos. Ventura C1,2, Bisphenol S Induces Changes in C. elegans Maturation, Vieira L1,2, Silva C1,2, Silva MJ1,2. 1National Institute of Health Reproduction, and Genome. Chung NJ, Lu J. Trinity School, Doutor Ricardo Jorge (INSA), Lisbon, Portugal, 2Center for New York, NY, United States. Toxicogenomics and Human Health (ToxOmics), NOVA Medical School-FCM, University of Lisbon (UNL), Lisbon, Portugal. P75 Determining the Association Between Genomic Instability P67 and Prevalence of Frailty Syndrome in Portuguese Older Epigenetic Effects of Early Life Aflatoxin Exposure in Rats. Adults. Costa S1,2, Teixeira-Gomes A1,2, Lage B1,2, Esteves F1,2, Dewald A1, Rotimi O2, Rotimi S2, Adelani IB2, Onuzulu C2, Loureiro A3, Valdiglesias V4, Laffon B4,Teixeira JP1,2. 1EPIUnit – Goodrich JM1. 1University of Michigan School of Public Health, Instituto de Saúde Pública da Universidade do Porto, Porto, Ann Arbor, MI, United States, 2Covenant University, Ota, Portugal, 2National Institute of Health, Porto, Portugal, 3Unidade Nigeria. de Saúde Familiar Esposende Norte, Esposende, Portugal, 4DICOMOSA Group, University of A Coruña, A Coruña, Spain. P68 miRNAs Associated With DNA Repair Are Upregulated By P76 Environmental Toxicants. Tsegay P, Lai Y, Liu Y. Florida Genotoxicity of Microplastics: Local Aquatic Biomonitoring International University, Miami, FL, United States. Suggests Burden of Environmental Plastic Pollution. Haley CE, Wardlaw C, Neff BD, Corcoran PL, de Souza CPE, Hill KA. P69 University of Western Ontario, London, ON, Canada. Neonatal Pb Exposure and DNA Methylation Profiles in Dried Bloodspots. Montrose L1, Goodrich JM2, Morishita M3, P77 Kochmanski J3, Cavalcante R2, Lumeng JC2, Peterson KE2, In Vivo Genotoxicity Assessment Of Multi-Wall Carbon Dolinoy DC2. 1Boise State University, Boise, ID, United States, Nanotubes Using Lung Micronucleus Assay. Horibata K1, 2University of Michigan, Ann Arbor, MI, United States, 3Michigan Takasawa H2, Taquahashi Y1, Yokota S1, Hamada S2,3, Honma State University, East Lansing, MI, United States. M1. 1National Institute of Health Sciences, Kanagawa, Japan, 2LSI Medience Corporation, Ibaraki, Japan, 3Bozo Research P70 Center Inc., Tokyo, Japan. POSTER CANCELLED: rs2278176 Contributes to the Favorable Prognosis for Metastatic Colorectal Cancer P78 Patients Treated with FOLFOX. Fu Y1, Wu S2, Yang X1, Sun Incidence of UVA and UVB Radiation and its Genotoxic H1, Xiaobo Li X1,2, Chen R1,2. 1Southeast University, Nanjing, Potential During 2018 in Southern Brazil. Cogo Borin B, P.R. China, 2Capital Medical University, Beijing, P.R. China. Schuch AP, Londero JEL. Federal University of Santa Maria(UFSM), Santa Maria, Brazil

Genotoxity Risk Assessment and P79 Influence of Antioxidant Capacity on Micronucleus Public Health Induction by Cigarette Smoke in Various Mammalian Cell Lines. Hashizume T, Yamamoto H, Shibuya K, Fukushima T. P71 Japan Tobacco Inc., Yokohama, Kanagawa, Japan. A Simple Electrophoretic Assay for Quantification of Genomic DNA Damage. Londero JEL, Schavinski CR, Silva P80 FD, Piccoli BC, Schuch AP. Federal University of Santa Maria, Mode-of-action Analysis of the Induction of Micronuclei by Santa Maria, RS, Brazil. a Flavouring Compound in Vitro. Munakata S, Watanabe T, Ishii T, Saito J, Erami K, Hashizume T. Japan Tobacco Inc., P72 Yokohama, Kanagawa, Japan. Analytical Pipeline for High-throughput gene Expression Profiling of per-and poly-fluoroalkyl Substances (PFAS) in P81 Primary Human Liver Cells Spheroids to Inform Read- Mycotoxins and Carcinogenesis: Disentangling Casual across. Rowan-Carroll A1, Reardon AJF1, Leingartner K1, 1 1 1 2 Links Through Epidemiological and Mechanistic Gagné R , Williams A , Kuo B , Bourdon-Lacombe JA , Moffat 1,2,3 1,3 2,3 2 2 1 3 4 1 Investigations. Claeys L , Huybrechts I , De Boevre M , I , Carrier R , Nong A , Lorusso L , Ferguson SS , Atlas E , 1,4 1 1 1 1 1 Zhivagui M , Casagrande C , Nicolas G , Renard C , Gunter Yauk CL . Environmental Health Science and Research 1 2,3 1 1 1 2 MJ , De Saeger S , Korenjak M , Zavadil J . IARC, Lyon, Bureau, Health Canada, Ottawa, ON, Canada, Water and Air France, 2Ghent University, Ghent, Belgium, 3Cancer Research Quality Bureau, Health Canada, Ottawa, ON, Canada, Institute Ghent (CRIG), Ghent, Belgium, 4Moore’s Cancer 3Chemicals and Environmental Health Management Bureau, 4 Center, University of California San Diego, San Diego, CA, Health Canada, Ottawa, ON, Canada, National Toxicology United States. Program, NIEHS, Research Triangle Park, NC, United States. P82 P73 Procarbazine-treated F344 Rats have CD48-deficient Bone Antigenotoxic Protective Effect Of Vitamin E And Of - Marrow Granulocytes with Mutations in the Pig-a gene. Dad Carotene Evaluated By The Comet Assay And The A, Revollo JR, Pearce MG, Heflich RH, Dobrovolsky VN. US 𝞫𝞫𝞫𝞫 Micronucleus Test In Female Recyclers Exposed To FDA, National Center for Toxicological Research, Jefferson, AR, Contaminants Of The Cateura Landfill In Asunción United States. Paraguay. Franco de Diana DM, Castiglioni DP, Figueredo R,

Yauk CL1, Marchetti F1. 1Environmental Health Science and P83 Research Bureau, Health Canada, Ottawa, ON, Canada; The Ames II Assay as a Component of the GeneTox21 2TwinStrand Biosciences, Inc., Seattle, WA, United States. high(er) Throughput in vitro Genetic Toxicity Assessment Platform. Chepelev NL, White PA. Environmental Health P91 Science and Research Bureau, Health Canada, Ottawa, ON, High Precision Risk Assessment System with Large-Scale Canada. Control Experiments in the Mouse. Gondo Y. Tokai University School of Medicine, Isehara, Japan. P84 The Use of the in vitro Micronucleus Assay on the P92 Genotoxicity Assessment of Cellulose Nanofibrils in Temporal Analysis of Mutant Frequencies in the Bone Mammalian Cell Lines. Silva MJ1,2, Ventura C1,2, Teixeira S1, Marrow of MutaMouse Males Supports the 28+28 Design as Marques C1, Vilar M1, Pinto F1,2, Lourenço AF3, Sousa-Mendes a Single Sampling Time for in vivo Mutagenesis. Zhou G, A4, Ferreira PJT3, Louro H1,2. 1National Institute of Health Dr. LeBlanc D, Williams A, Yauk CL, Douglas GR, Marchetti F. Ricardo Jorge, Lisbon, Portugal, 2Center for Toxicogenomics Environmental Health Science and Research Bureau, Health and Human Health (ToxOmics), NOVA Medical School, New Canada, Ottawa, ON, Canada. University of Lisbon, Lisbon, Portugal, 3University of Coimbra, CIEPQPF, Coimbra, Portugal, 4RAIZ – Forest and Paper P93 Research Institute, Aveiro, Portugal. The in vivo Pig-a Mutation Assay is Feasible in Primary Murine Liver Cells. Lichtler RC, Wilson MJ, Wickliffe JK. Tulane University, New Orleans, LA, United States. P85 Validation of CACO-2/HT29-MTX Model to Assess the P94 Potential Risk of Ingested Titanium Dioxide Nanoparticles. The Rate and Spectrum of EMS-induced Mutations in the Rolo D1, Pereira JFS1,2, Matos P1,2, Vieira A1, Vital N1, Jordan Microcrustacean Daphnia: On the Prospect of Forward P1,2, Silva MJ1,3, Louro H1,3. 1National Institute of Health Dr. Genetics. Ricardo Jorge (INSA), Lisbon, Portugal, 2BioISI –Biosystems & Snyman M, Xu S. University of Texas at Arlington, Arlington, TX, Integrative Sciences Institute, Faculty of Sciences, University of United States. Lisbon, Lisbon, Portugal, 3ToxOmics – Centre for Toxicogenomics and Human Health, NOVA Medical School, NOVA University, Lisbon, Portugal. Other

P95 Germ Cell and Heritable Effects Characterization of Rat Testicular Organoids Exposed to Ionizing Radiation. Petibone DM, Chen Y, Shemansky JM, P86 Muskhelishvili L, and Elespuru RK. National Center for Effect of Repeated Exposure to Glyphosate on Drosophila Toxicological Research, US FDA, Silver Spring, MD, United Melanogaster for Fourteen Generations. Rivas-Martínez H, States. Ramos-Morales P. UNAM, México, DF, Mexico. P96 P87 Dysregulation of NTHL1 and Sensitivity to Exposure of Drosophila Melanogaster Females and Males Chemotherapeutic Agents in Human Cells. Kar A, to Mitomycin (MMC) Produces a Different Degtyareva NP, Doetsch PW. NIEHS, Durham, NC, United Transgenerational Effect. Arroyo Jilote E, Ramos-Morales P. States. UNAM, Mexico, DF, Mexico. P97 P88 Mechanisms of Activation and Detoxification of Scrutinizing Variants of Uncertain Significance in the PMS2 Aristolochic Acid and 3-Nitrobenzanthrone. Sidorenko VS, Gene. D'Arcy BM1, Lange CR1, Arrington JF1, Weissman J1, Moriya M. Stony Brook University, Stony Brook, NY, United Blount J2, Prakash A1. 1University of South Alabama, Mobile, States. AL, United States, 2Circulogene Theranostics, Birmingham, AL, United States. P98 Novel LRRK2 Transgenic Mouse Model to Understand Gene P89 x Environment Interactions. Goodson SD, Colton CA, Smoking and Mutations in Sperm DNA. Axelsson J1,2, Sanders LH. Duke University Medical Center, Durham, NC, Romerius P1, Rignell-Hydbom A2, Engström K2. 1Skåne United States. University Hospital, Lund, Sweden, 2Lund University, Lund, Sweden. P99 POSTER CANCELLED: Targeting Spike Glycoprotein of SARS-corona virus-2 with Chaga Mushroom: An in silico In Vivo Mutagenesis Study for Developing a Promising Natural Therapeutic. Ibrahim J1, Das B2, Basal WT1. 1Cairo University, Cairo, Egypt, 2 P90 KIIT University, Bhubaneswar, India Duplex Sequencing™ Demonstrates Dose-related Increases in Mutation Frequencies and C>A Transitions in the Bone Marrow of MutaMouse Males Exposed to Benzo(a)pyrene. LeBlanc D1, Meier M1, Buick J1, Schmidt EK2, Yin Lo F2, Norgaard Z2, Williams L2, Valentine CC2, Salk JJ2,

The Genomics and Data Sciences P104 Optimizing a Bacterial Laccase for Effective Oxidation of P100 Genotoxic Emerging Contaminants in Wastewater: An in silico Approach. Chen JH1, Varghese N1, McGregor L2, Jiang A Cross-platform and Inter-laboratory Evaluation of the 1 1 1 1 1 1 J , Karas B , Hill KA . University of Western Ontario, London, TGx-DDI Transcriptomic Biomarker. Williams A , Buick JK , 2 Cho E1,2, Chen R3, Li HH3, Fornace Jr AJ3,4, Allemang A5, ON, Canada, McMaster University, Hamilton, ON, Canada. Pfuhler S5, Frötschl R6, Ellinger-Ziegelbauer H7, Aubrecht J8, Pettit SD9, Yauk CL1,2. 1Environmental Health Science and P105 TCDD-inducible Poly-ADP-ribose Polymerase Regulates Research Bureau, Health Canada, Ottawa, ON, Canada, 1 1 2Carleton University, Ottawa, ON, Canada, 3Lombardi AHR Biology and Dioxin Toxicity in Rat. Long AS , Hutin D , Norell PN2, Sugamori KS1, Shao P1, Grant DM1, Matthews, J3. Comprehensive Cancer Center, Georgetown University Medical 1 2 Center, Washington, DC, United States 4Georgetown University University of Toronto, Toronto, ON, Canada, Institute of 5 Environmental Medicine, Karolinska Institute, Stockholm, Medical Center, Washington, DC, United States, Procter & 3 Gamble, Mason, OH, United States, 6Federal Institute for Drugs Sweden, Institute of Basic Medical Sciences, University of and Medical Devices, Bonn, Germany, 7Bayer AG, Wuppertal, Oslo, Norway. Germany, 8Takeda Pharmaceuticals USA Inc., Cambridge, MA, United States, 9Health and Environmental Sciences Institute, P106 Washington, DC, United States. POSTER CANCELLED: Variation in mRNA Expression Patterns Explains Phenotypic Variation in Whole Animal P101 Metabolism, Thermal Tolerance, and Cardiac Metabolic A Robust Analysis Pipeline for Lineage-based Detection of Rate of Fundulus Heteroclitus Populations Experiencing Mosaic Copy Number Variants in Single Cells. Wang K, Variable Local Temperatures. Drown MK, Crawford DL, Ahmed S, Sartor MA, Corfas G, Hammoud SS, Sekiguchi JM, Oleksiak MF. University of Miami, Miami, FL, United States. Kidd JM, Glover TW, Wilson TE. University of Michigan Medical School, Ann Arbor, MI, United States. Late-Breaking Abstract P102 P107 Chemogenomic Screening Identifies the Hsp70 co- chaperone DNAJA1 as a Hub for Anticancer Drug Long-term Effects Of Wildfire Exposure During Early . Nitika N1, Blackman JS1, Knighton LE1, Takakuwa Childhood On The Methylome In Rhesus Macaques. Resistance 1 1 1 2 1,3 JE1, Calderwood SK2, Truman AW1. 1University of North Brown AP , Cai L , Pham C , Laufer BI , Miller LA , Carolina Charlotte, Charlotte, NC, United States, 2Beth Israel LaSalle JM2, Ji H1,3. 1California National Primate Deaconess Medical Center, Harvard Medical School, Boston, Research Center, Davis, CA, United States, 2MIND MA, United States. Institute, Genome Center, Department of Medical Microbiology and Immunology, University of California, P103 Davis, CA United States, 3School of Veterinary Investigating Genomic and Mutational Signatures of Medicine, Department of Anatomy, Physiology and Cell Organisms Living in Extreme Environments. Soltysiak MPM1, Randhawa GS1, Osman A1, de Souza CPE1, Hill KA1, biology, University of California, Davis, CA United Kari L2. 1University of Western Ontario, London, ON, Canada, States. 2University of Waterloo, Waterloo, ON, Canada.

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