DOCSLIB.ORG

(3Α-DIOL G) ELISA Absent

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(3Α-DIOL G) ELISA Absent Diagnostics Biochem Canada Inc. 2. Control materials or serum pools should be included in standards and controls has been tested and found to be non- *Listed below are approximate concentrations, please refer Manufacturing Innovative IVD for the World every run at a high and low level for assessing the reactive for Hepatitis B surface antigen and has also been to bottle labels for exact concentrations. Since 1973 reliability of results. tested for the presence of antibodies to HCV and Human 3. When the use of water is specified for dilution or Immunodeficiency Virus (HIV) and found to be negative. Calibrator Concentration Volume 5α-ANDROSTANE-3α, 17β DIOL reconstitution, use deionized or distilled water. No test method however, can offer complete assurance that Calibrator A 0 ng/mL 2.0 mL 4. In order to reduce exposure to potentially harmful HIV, HCV and Hepatitis B virus or any infectious agents are Calibrator B 0.25 ng/mL 0.6 mL GLUCURONIDE (3α-DIOL G) ELISA absent. The reagents should be considered a potential bio- substances, gloves should be worn when handling kit Calibrator C 1 ng/mL 0.6 mL hazard and handled with the same precautions as applied to For Research Use Only. Not for reagents and human specimens. Calibrator D 3 ng/mL 0.6 mL EU: CAN: USA: Use in Diagnostic Procedures any blood specimen. 5. All kit reagents and specimens should be brought to Calibrator E 10 ng/mL 0.6 mL room temperature and mixed gently but thoroughly before use. Avoid repeated freezing and thawing of reagents CHEMICAL HAZARDS Calibrator F 50 ng/mL 0.6 mL Version 6.0 : CAN-DG-460 and specimens. Avoid contact with reagents containing TMB, hydrogen peroxide Storage: Refrigerate at 2–8°C Effective: May 15, 2017 6. A calibrator curve must be established for every run. and sulfuric acid. If contacted with any of these reagents, Stability: 12 months in unopened vials or as indicated on 7. The controls should be included in every run and fall wash with plenty of water. TMB is a suspected carcinogen. label. Once opened, the standards should be INTENDED USE within established confi dence limits. used within 14 days or aliquoted and stored frozen. Avoid multiple freezing and thawing cycles. For the direct quantitative determination of 3α-Diol G by 8. Improper procedural techniques, imprecise pipetting, SPECIMEN COLLECTION AND STORAGE an enzyme immunoassay in human serum. incomplete washing as well as improper reagent storage Approximately 0.2 mL of serum is required per duplicate may be indicated when assay values for the controls do determination. Collect 4–5 mL of blood into an appropriately 4. Controls – Ready to Use not refl ect established ranges. PRINCIPLE OF THE TEST labelled tube and allow it to clot. Centrifuge and carefully Contents: Two vials containing 3α-Diol G in a protein-based 9. When reading the microplate, the presence of bubbles remove the serum layer. Store at 4°C for up to 24 hours or at buffer with a non-mercury preservative. Prepared The principle of the following enzyme immunoassay test in the microwells will affect the optical densities (ODs). -10°C or lower if the analyses are to be done at a later date. follows the typical competitive binding scenario. Competition by spiking bufer with defi ned quantities of 3α-Diol G. Carefully remove any bubbles before performing the Consider all human specimens as possible biohazardous Refer to vial labels for the acceptable range. occurs between an unlabelled antigen (present in standards, reading step. materials and take appropriate precautions when handling. controls and patient samples) and an enzyme-labelled antigen 10. The substrate solution (TMB) is sensitive to light and Volume: 0.6 mL/vial (conjugate) for a limited number of antibody binding sites on should remain colourless if properly stored. Instability or Storage: Refrigerate at 2–8°C. the microplate. The washing and decanting procedures remove contamination may be indicated by the development of a SPECIMEN PRETREATMENT Stability: 12 months in unopened vials or as indicated on unbound materials. After the washing step, the enzyme blue colour, in which case it should not be used. This assay is a direct system; no specimen pretreatment label. Once opened, the controls should be used substrate is added. The enzymatic reaction is terminated by 11. The assay buffer is sensitive to light and should be stored is necessary. within 14 days or aliquoted and stored frozen. addition of the stopping solution. The absorbance is measured in the original dark bottle away from direct sunlight. Avoid multiple freezing and thawing cycles. on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of 3α-Diol G in 12. When dispensing the substrate and stopping solution, do REAGENTS AND EQUIPMENT NEEDED BUT NOT the sample. A set of standards is used to plot a standard not use pipettes in which these liquids will come into contact PROVIDED 5. Wash Buffer Concentrate – Requires Preparation X10 with any metal parts. curve from which the amount of 3α-Diol G in patient samples 1. Precision pipettes to dispense 50, 100, 150 and 300 μL Contents: One bottle containing buffer with a non-ionic and controls can be directly read. 13. To prevent contamination of reagents, use a new disposable 2. Disposable pipette tips detergent and a non-mercury preservative. pipette tip for dispensing each reagent, sample, standard 3. Distilled or deionized water and control. Volume: 50 mL/bottle CLINICAL APPLICATIONS 4. Plate shaker 14. Do not mix various lot numbers of kit components within Storage: Refrigerate at 2–8°C 5α-Androstane-3α, 17β-diol glucuronide is a C19 steroid and 5. Microplate reader with a fi lter set at 450 nm and an upper a test and do not use any component beyond the expiration Stability: 12 months or as indicated on label. is either abbreviated as 3α-Diol G, 5α-Diol G or simply, α-Diol G. date printed on the label. OD limit of 3.0 or greater* (see assay procedure step 10) It is produced mainly as a metabolite of testosterone and Preparation: Dilute 1:10 in distilled or deionized water before 15. Kit reagents must be regarded as hazardous waste and use. If the whole plate is to be used dilute 50 mL dihydrotestosterone (DHT). It is largely produced in target REAGENTS PROVIDED peripheral tissues such as the skin, especially around hair disposed of according to national regulations. of the wash buffer concentrate in 450 mL of water. follicles. The stimulation by large amounts of 3α-Diol G leads 1. Rabbit Anti-3α-Diol G Antibody-Coated Break-Apart Well Microplate – Ready To Use to excessive hair formation, notably where hair is not normally LIMITATIONS 6. Assay Buffer – Ready to Use* present in women. 1. All the reagents within the kit are calibrated for the direct Contents: One 96-well (12x8) polyclonal antibody-coated Contents: One bottle containing a protein-based buffer with determination of 3α-Diol G in human serum. The kit is microplate in a resealable pouch with desiccant. In recent years the interest in the measurement of this steroid a non-mercury preservative. has increased among clinical investigators studying women not calibrated for the determination of 3α-Diol G in saliva, Storage: Refrigerate at 2–8°C. Volume: 15 mL/bottle suffering from idiopathic hirsutism. plasma or other specimens of human or animal origin. Stability: 12 months or as indicated on label. Among the steroids known to be precursors for 3α-Diol G 2. Do not use grossly hemolyzed, grossly lipemic, icteric or Storage: Refrigerate at 2–8°C improperly stored serum. are dehydroepiandrosterone (DHEA), dehydroepiandrosterone 2. 3α-Diol G-Horseradish Peroxidase (HRP) Conjugate Stability: 12 months or as indicated on label. sulphate (DHEAS), dihydrotestosterone (DHT), androstenedione 3. Any samples or control sera containing azide or thimerosal Concentrate — Requires Preparation X50 *Warm to completely dissolve before use. and testosterone. Only 3α-Diol G has been shown to increase are not compatible with this kit, as they may lead to false with hirsutism and decrease with treatment. This correlation results. Contents: 3α-Diol G-HRP conjugate in a protein-based buffer with a non-mercury preservative. has also been demonstrated in patients with polycystic 4. Only calibrator A may be used to dilute any high serum 7. TMB Substrate – Ready To Use ovarian syndrome (PCO). 3α-Diol G determinations have samples. The use of any other reagent may lead to false Volume: 300 μL/vial Contents: One bottle containing tetramethylbenzidine and therefore proved to be a useful indicator in a variety of ways results. Storage: Refrigerate at 2–8°C hydrogen peroxide in a non-DMF or DMSO including monitoring the progress of treatment of idiopathic 5. The results obtained with this kit should never be used as Stability: 12 months or as indicated on label. containing buffer. hirsutism and women with PCO. the sole basis for a clinical diagnosis. For example, the Preparation: Dilute 1:50 in assay buffer before use (eg. Volume: 16 mL/bottle Furthermore, diabetic patients (both men and women) under occurrence of heterophilic antibodies in patients regularly 40 μL of HRP in 2 mL of assay buffer). If the Storage: Refrigerate at 2–8°C cyclosporine A therapy have shown increased 3α-Diol G exposed to animals or animal products has the potential of whole plate is to be used dilute 240 μL of HRP levels, a side effect resulting in the appearance of hair in Stability: 12 months or as indicated on label. causing interferences in immunological tests. Consequently, in 12 mL of assay buffer.
Load more

Recommended publications
  • Dehydroepiandrosterone – Is the Fountain of Youth Drying Out?
    Physiol. Res. 52: 397-407, 2003 MINIREVIEW Dehydroepiandrosterone – Is the Fountain of Youth Drying Out? P. CELEC 1,2, L. STÁRKA3 1Faculty of Medicine, 2Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia and 3Institute of Endocrinology, Prague, Czech Republic Received September 15, 2002 Accepted October 7, 2002 Summary Dehydroepiandrosterone (DHEA) and its sulphate-bound form (DHEAS) are important steroids mainly of adrenal origin. Their physiological and pathophysiological functions are not yet fully identified, although a number of various possible features have been hypothesized. Most popular is the description of the “hormone of youth” as the long-term dynamics of DHEA levels are characterized by a sharp age-related decline in the late adulthood and later. Low levels of DHEA are, however, associated not only with the ageing process but also with diabetes mellitus, cardiovascular diseases and some neurological or immunological entities. In the past decade, a number of brief studies have concentrated on these relationships and also on the role of exogenous DHEA in health, disease and human well-being. This article tries to summarize some of the most important facts achieved recently. Key words Dehydroepiandrosterone • Intracrinology • Hormone replacement therapy • Steroids Introduction functions: 1) DHEA is an endogenous metabolite that cannot be patented so that pharmaceutical companies are In 1934 Butenandt and Dannenbaum isolated not interested in supporting research in this field. dehydroepiandrosterone (DHEA) from urine and in 1944 2) DHEA can be described as a “human molecule” Munson and colleagues identified its 3β-sulphate because other investigated species have much lower (DHEAS). Even now, nearly 70 years later, we still do concentrations.
    [Show full text]
  • Dihydrotestosterone (Adractim®) 2.5% Gel for Topical Application
    d g t squeezin l aa StSt gelgel Star gel from this en 0mg0m0mg 2.2.55 5mg5mg 7.7.55 10m1 mg l l 12.12.55 ge g ge ) 15m15mg f 17.17.55 ® 20m20mg g g o 22.22.55 25 25m25mg 1.25 g of g 1.25 1 1.25 1 1 1.25 g of g 1.25 27.5 30mg300mm 32.32.5.5 35m35mg drotestosterone hy 37.5 Put on a pair of gloves Gently squeeze the gel onto the mark on the ruler illustrated below – as instructed on the dispensing label. Leave for five minutes Wipe this laminated information sheet with a damp piece of kitchen paper ready for the next dose. Remove the gloves and wash them in warm soapy water ready for the next dose. of di 40m40mg © GOSH NHS Foundation Trust January 2016 © GOSH NHS Foundation Trust How is it used? Dihydrotestosterone 2.5% gel is for external It should be applied over the use only. required area of skin after washing. The gel should be left to dry for five minutes or so before putting on clothes. 1. 2. 3. Spread over the required area evenly 4. 5. Note: Dihydrotestosterone 2.5% gel should not be applied to any broken areas of skin. 42.42.55 l l 45mg45m45m ge ge 47.47.55 , ® 50mg50mm 52.52.55 55mg55mm 57.57.55 2.5 g of g 2 2.5 2 2.5 g of g 2.5 60mg600m0m 62.62.5.5 Dihydrotestosterone is a synthetic version of a hormone called testosterone.
    [Show full text]
  • Spironolactone 2021 Newborn Use Only
    Spironolactone 2021 Newborn use only Alert Spironolactone is a potassium-sparing diuretic and concomitant intake of potassium or ACE inhibitors may lead to hyperkalemia. Indication Diuretic primarily prescribed for its potassium-sparing effect. For heart failure, in conjunction with furosemide. For chronic lung disease, in conjunction with a thiazide diuretic. Bartter syndrome and Gitelman Syndrome. Action Spironolactone is a synthetic steroid that acts as a competitive aldosterone receptor antagonist, so inhibits sodium reabsorption and spares potassium. It is a weak diuretic. It also inhibits the interaction between dihydrotestosterone and the intracellular androgen receptor resulting in moderate antiandrogenic activity. Drug type Non-selective mineralocorticoid receptor antagonist. Trade name Aldactone; Spiractin Presentation Oral suspension prepared in pharmacy: 1 mg/mL; 2.5 mg/mL; 5 mg/mL. Dose 1–3 mg/kg/dose 24 hourly. Dose can be divided into different intervals. Dose adjustment Therapeutic hypothermia – Not applicable. Renal impairment – Refer to precautions section. Hepatic impairment – Refer to precautions section. Maximum dose 3 mg/kg/day Total cumulative dose Route Oral Preparation Oral suspension. Administration Administer undiluted with feeds. Monitoring Serum potassium at regular intervals. Contraindications Hyperkalaemia. Significant renal impairment. Anuria. Adrenal insufficiency. Precautions Use with caution in infants with renal or hepatic impairment. Monitor more frequently if infant is also given potassium. Drug interactions Spironolactone increases the effects of ACE inhibitors (leading to hyperkalemia), digoxin and sotalol. Adverse reactions Hyperkalaemia and metabolic acidosis. Antiandrogenic effects include reduced hirsutism and gynecomastia. There is one case report of an ovarian cyst in a neonate on spironolactone. Spironolactone interferes with 17-hydroxyprogesterone measurement, which is used to screen neonates for congenital adrenal hyperplasia.
    [Show full text]
  • DHEA Sulfate, and Aging: Contribution of the Dheage Study to a Sociobiomedical Issue
    Dehydroepiandrosterone (DHEA), DHEA sulfate, and aging: Contribution of the DHEAge Study to a sociobiomedical issue Etienne-Emile Baulieua,b, Guy Thomasc, Sylvie Legraind, Najiba Lahloue, Marc Rogere, Brigitte Debuiref, Veronique Faucounaug, Laurence Girardh, Marie-Pierre Hervyi, Florence Latourj, Marie-Ce´ line Leaudk, Amina Mokranel, He´ le` ne Pitti-Ferrandim, Christophe Trivallef, Olivier de Lacharrie` ren, Stephanie Nouveaun, Brigitte Rakoto-Arisono, Jean-Claude Souberbiellep, Jocelyne Raisonq, Yves Le Boucr, Agathe Raynaudr, Xavier Girerdq, and Franc¸oise Foretteg,j aInstitut National de la Sante´et de la Recherche Me´dicale Unit 488 and Colle`ge de France, 94276 Le Kremlin-Biceˆtre, France; cInstitut National de la Sante´et de la Recherche Me´dicale Unit 444, Hoˆpital Saint-Antoine, 75012 Paris, France; dHoˆpital Bichat, 75877 Paris, France; eHoˆpital Saint-Vincent de Paul, 75014 Paris, France; fHoˆpital Paul Brousse, 94804 Villejuif, France; gFondation Nationale de Ge´rontologie, 75016 Paris, France; hHoˆpital Charles Foix, 94205 Ivry, France; iHoˆpital de Biceˆtre, 94275 Biceˆtre, France; jHoˆpital Broca, 75013 Paris, France; kCentre Jack-Senet, 75015 Paris, France; lHoˆpital Sainte-Perine, 75016 Paris, France; mObservatoire de l’Age, 75017 Paris, France; nL’Ore´al, 92583 Clichy, France; oInstitut de Sexologie, 75116 Paris, France; pHoˆpital Necker, 75015 Paris, France; qHoˆpital Broussais, 75014 Paris, France; and rHoˆpital Trousseau, 75012 Paris, France Contributed by Etienne-Emile Baulieu, December 23, 1999 The secretion and the blood levels of the adrenal steroid dehydro- number of consumers. Extravagant publicity based on fantasy epiandrosterone (DHEA) and its sulfate ester (DHEAS) decrease pro- (‘‘fountain of youth,’’ ‘‘miracle pill’’) or pseudoscientific asser- foundly with age, and the question is posed whether administration tion (‘‘mother hormone,’’ ‘‘antidote for aging’’) has led to of the steroid to compensate for the decline counteracts defects unfounded radical assertions, from superactivity (‘‘keep young,’’ associated with aging.
    [Show full text]
  • UPLC-MS/MS Analysis of Dihydrotestosterone, Dehydroepiandrosterone, Testosterone, Androstenedione, 17-Hydroxyprogesterone
    [ APPLICATION NOTE ] UPLC-MS/MS Analysis of Dihydrotestosterone, Dehydroepiandrosterone, Testosterone, Androstenedione, 17-Hydroxyprogesterone, and Progesterone in Serum for Clinical Research Dominic Foley and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION BENEFITS INTRODUCTION ■■ Analytical selectivity of the Steroid hormones encompass a large class of small molecules that play a central chromatographic method provides role in metabolic processes, such as regulation of sexual characteristics, blood separation of isobaric species pressure, and inflammation. Measurement of these steroids by immunoassay can be prone to analytical interference as a result of cross reactivity of ■■ UPLC-MS/MS enables high sample-throughput using reagent antibodies with structurally related steroid hormones and synthetic multi-well plate automation derivatives. UltraPerformance LC™ - tandem mass spectrometry–tandem mass spectrometry (UPLC-MS/MS) can provide analytically sensitive, ■■ Excellent agreement to EQA accurate, and precise measurement of these steroid hormones. mean values for testosterone, androstenedione, 17-OHP, Here we describe a clinical research method utilizing Oasis MAX and DHT µElution Plate technology for the extraction of Dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), testosterone, androstenedione, 17-Hydroxyprogesterone (17-OHP), and progesterone from serum, which has been automated using the Tecan Freedom EVO 100/4 liquid handler. Chromatographic separation was performed on an ACQUITY UPLC I-Class System using a CORTECS UPLC C18 Column, followed by detection on WATERS SOLUTIONS a Xevo TQ-S micro Mass Spectrometer (Figure 1). In addition, we have Oasis™ MAX µElution Plate examined External Quality Assessment (EQA) samples for testosterone, androstenedione, 17-OHP, and DHT to evaluate the bias and therefore CORTECS™ UPLC™ C18 Column suitability of the method for analyzing these steroids for clinical research.
    [Show full text]
  • Binding of Androgen- and Estrogen-Like Flavonoids to Their Cognate (Non)Nuclear Receptors: a Comparison by Computational Prediction
    molecules Article Binding of Androgen- and Estrogen-Like Flavonoids to Their Cognate (Non)Nuclear Receptors: A Comparison by Computational Prediction Giulia D’Arrigo 1, Eleonora Gianquinto 1, Giulia Rossetti 2,3,4 , Gabriele Cruciani 5, Stefano Lorenzetti 6,* and Francesca Spyrakis 1,* 1 Department of Drug Science and Technology, University of Turin, Via Giuria 9, 10125 Turin, Italy; [email protected] (G.D.); [email protected] (E.G.) 2 Institute for Neuroscience and Medicine (INM-9) and Institute for Advanced Simulations (IAS-5) “Computational Biomedicine”, Forschungszentrum Jülich, 52425 Jülich, Germany 3 Jülich Supercomputing Center (JSC), Forschungszentrum Jülich, 52425 Jülich, Germany 4 Department of Neurology, RWTH, Aachen University, 52074 Aachen, Germany; [email protected] 5 Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy; [email protected] 6 Istituto Superiore di Sanità (ISS), Department of Food Safety, Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161 Rome, Italy * Correspondence: [email protected] (S.L.); [email protected] (F.S.) Abstract: Flavonoids are plant bioactives that are recognized as hormone-like polyphenols because of Citation: D’Arrigo, G.; Gianquinto, their similarity to the endogenous sex steroids 17β-estradiol and testosterone, and to their estrogen- E.; Rossetti, G.; Cruciani, G.; and androgen-like activity. Most efforts to verify flavonoid binding to nuclear receptors (NRs) and Lorenzetti, S.; Spyrakis, F. Binding of explain their action have been focused on ERα, while less attention has been paid to other nuclear Androgen- and Estrogen-Like and non-nuclear membrane androgen and estrogen receptors. Here, we investigate six flavonoids Flavonoids to Their Cognate (apigenin, genistein, luteolin, naringenin, quercetin, and resveratrol) that are widely present in fruits (Non)Nuclear Receptors: A and vegetables, and often used as replacement therapy in menopause.
    [Show full text]
  • Don't Let Your Hair Down
    Hair ™ Patent N° WO 00/58347 PROCAPIL Don't let your Biotinyl-GHK, citrus and olive tree leaves hair down Function: Fights follicle ageing and protects against hair loss. Definition: Combination of a vitamin fortified matrikine™ (biotinyl-GHK) with apigenin (a flavonoid from citrus) and oleanolic acid from olive tree leaves. Properties: PROCAPILTM targets the main causes of alopecia: poor scalp micro-circulation, follicle ageing and follicle atrophy caused by dihydrotestosterone. Characteristics: Biotinyl-GHK stimulates cell metabolism, apigenin improves micro-circulation and oleanolic acid inhibits 5α-reductase. INCI name: Butylene Glycol – Water (Aqua) – PPG-26-Buteth-26 – PEG-40 Hydrogenated Castor Oil – Apigenin – Oleanolic Acid – Biotinoyl Tripeptide-1 Applications: Hair care products: shampoos, conditioners, leave-on, hair lotions, masks... Formulation: Fortifies Water soluble. Incorporate at 45°C in emulsions or at room temperature in gels. Rejuvenates Recommended use level: 1% to 2% Fights against hair loss Member of Croda International Plc Copyright© 2006 Sederma. All rights reserved. www.sederma.fr E-mail: [email protected] CLAIM SUBSTANTIATION April 2004 Stimulation of cell metabolism In vitro Examples of activated genes by PROCAPILTM Mitosis rate Gene Activity Activation Evaluation of root sheath keratinocytes after a Laminin binding protein Adhesion +146% 14-day culture of hair follicles. Biotiny-GHK (2 ppm) stimulates Ki-67 expression, indicating enhanced cell Acetyl CoA transferase Cell metabolism +137% proliferation. Cytokeratins 10 Differentiation +154% Gene expression Morphological observation PROCAPILTM promotes the expression of numerous control PROCAPILTM genes involved in tissue repair mechanisms (DNA-array on 3D SkinEthic® epidermis). Hair Root sheath Hair anchoring Hair follicles are incubated for 14 days with biotinyl-GHK Dermis / Root sheath (2 ppm).
    [Show full text]
  • Metabolism of Androstenone, 17Β- Estradiol and Dihydrotestosterone
    Metabolism of Androstenone, 17β- Estradiol and Dihydrotestosterone in Primary Cultured Pig Hepatocytes and the Role of 3β-Hydroxysteroid Dehydrogenase in This Process Chen, G. , Zhu, D. , Li, Y. , Bai, Y. , Fang, M. , Ren, L. and Al- Kateb, H. Published PDF deposited in Curve February 2016 Original citation: Chen, G. , Zhu, D. , Li, Y. , Bai, Y. , Fang, M. , Ren, L. and Al-Kateb, H. (2015) Metabolism of Androstenone, 17β-Estradiol and Dihydrotestosterone in Primary Cultured Pig Hepatocytes and the Role of 3β-Hydroxysteroid Dehydrogenase in This Process. PLoS ONE, volume 10 (1): e113194 URL: http://dx.doi.org/10.1371/journal.pone.0113194 DOI: 10.1371/journal.pone.0113194 Publisher: Public Library of Science This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders. CURVE is the Institutional Repository for Coventry University http://curve.coventry.ac.uk/open Metabolism of Androstenone, 17b-Estradiol
    [Show full text]
  • View the Active Ingredients List
    Active Ingredients Anti-Aging is a complicated market; at Ethica we believe in sharing the ingredients work with, so our customers can understand the products they are using. All the marketing in the world means nothing without the correct formulation. We do not “fairy dust” our products to say something is in there, if it is not providing a result that is backed by clinical studies, we leave it out. We are open with our ingredients, why they are there and what they do. Anti-Aging and hair regrowth is extremely complicated and technical, there is no single ingredient that is the perfect solution, that is why using a precise combination of the correct ingredients will yield maximum results across multiple pathways for the broadest range of people. Lets take a look at many of our advanced ingredients: Adenosine Emu Oil Performs similar to 5% minoxidil for hair loss, recent scientif- A powerful anti-inflammatory, emu oil is also very highly ic studies have also show that Adenosine, which is native to absorbent. Emu Oil has also been shown to be a strong the human body, in comparison to Minoxidil and Finasteride, 5 alpha reductase inhibitor or DHT, in target tissues when has no side effects. A 12-month usage of Adenosine leads topically applied to an improvement of hair condition in 84% of all cases Extrapone Rooibos Anageline Extrapone® Rooibos GW (by Symrise) acts as an antioxi- ANAGELINE® is extracted from sweet white lupine. Rich dant. It is a plant extract derived from Aspalathus Linearis in glutaminated peptides, oligo-elements and vitamins, it and possesses anti-aging, anti-allergenic, antimicrobial, pro- controls the hair cycle by regulating the hormonal balance, tective and skin soothing properties.
    [Show full text]
  • Documentation, Codebook, and Frequencies
    Documentation, Codebook, and Frequencies Surplus Sera Laboratory Component: Racial/Ethnic Variation In Sex Steroid Hormone Concentrations Across Age In US Men Survey Years: 1988 to 1991 SAS Export File: SSHORMON.XPT First Published: October 2006 Last revised: N/A NHANES III Data Documentation Laboratory Assessment: Racial/Ethnic Variation in Sex Steroid Hormone Concentrations Across Age In US Men (NHANES III Surplus Sera) Years of Coverage: 1988-1991 First Published: October 2006 Last Revised: N/A Introduction It has been proposed that racial/ethnic variation in prostate cancer incidence may be, in part, due to racial/ethnic variation in sex steroid hormone levels. However, it remains unclear whether in the US population circulating concentrations of sex steroid hormones vary by race/ethnicity. To address this, concentrations of testosterone, sex hormone binding globulin, androstanediol glucuronide (a metabolite of dihydrotestosterone) and estradiol were measured in stored serum specimens from men examined in the morning sample of the first phase of NHANES III (1988-1991). This data file contains results of the testing of 1637 males age 12 or more years who participated in the morning examination of phase 1 of NHANES III and for whom serum was still available in the repository. Data Documentation for each of these four components is given in sections below. I. Testosterone Component Summary Description The androgen testosterone (17β -hydroxyandrostenone) has a molecular weight of 288 daltons. In men, testosterone is synthesized almost exclusively by the Leydig cells of the testes. The secretion of testosterone is regulated by luteinizing hormone (LH), and is subject to negative feedback via the pituitary and hypothalamus.
    [Show full text]
  • (SOGC) Clinical Practice Guideline
    Journal of Obstetrics and Gynaecology Canada The official voice of reproductive health care in Canada Le porte-parole officiel des soins génésiques au Canada Journal d’obstétrique et gynécologie du Canada Volume 36, Number 9 • volume 36, numéro 9 September • septembre 2014 Supplement 2 • supplément 2 Abstract . S1 Chapter 1: Assessment and Risk Management of Menopausal Women . S6 Chapter 2: Cardiovascular Disease . S16 Chapter 3: Menopausal Hormone Therapy and Breast Cancer . S23 Chapter 4: Vasomotor Symptoms . S31 Chapter 5: Urogenital Health . S35 Chapter 6: Managing Prescription Therapeutic Agents . S42 Chapter 7: Menopause Ongoing Management of Menopausal Women and Those With Special Considerations . S51 Chapter 8: Sexuality and Menopause . S59 Chapter 9: Complementary and Alternative Medicine (CAM) . S74 Publications mailing agreement #40026233. Return undeliverable Canadian copies and change of address notifications to SOGC Subscriptions Services, 780 Echo Dr. Ottawa, Ontario K1S 5R7. Editor-in-Chief / Rédacteur en chef Timothy Rowe CPL Editor / Rédactrice PPP Vyta Senikas Translator / Traducteur Martin Pothier Assistant Editor / Rédactrice adjointe Jane Fairbanks Editorial Assistant / Adjointe à la rédaction Daphne Sams Editorial Office / Bureau de la rédaction Journal of Obstetrics and Gynaecology Canada Room D 405A BC Women's Hospital 4500 Oak Street Vancouver BC V6H 3N1 [email protected] Tel: (604) 875-2424 ext. 5668 Fax: (604) 875-2590 http://www.jogc.com The Journal of Obstetrics and Gynaecology Canada (JOGC) is owned by the Society of Obstetricians and Gynaecologists of Canada (SOGC), published by the Canadian Psychiatric Association (CPA), and printed by The Lowe-Martin Group, Ottawa, ON. Le Journal d’obstétrique et gynécologie du Canada (JOGC), qui relève de la Société des obstétriciens et gynécologues du Canada (SOGC), est publié par l’Association des psychiatres du Canada (APC), et imprimé par The Lowe-Martin Group, Ottawa (Ontario).
    [Show full text]
  • Serum Levels of 3A-Androstanediol Glucuronide in Hirsute and Non Hirsute Women
    European Journal of Endocrinology (1998) 138 421–424 ISSN 0804-4643 Serum levels of 3a-androstanediol glucuronide in hirsute and non hirsute women L Falsetti, B Rosina and D De Fusco Department of Gynaecological Endocrinology, University of Brescia, Italy (Correspondence should be addressed to L Falsetti, Via Tirandi, 13-scala F, 25128 Brescia, Italy) Abstract This study has evaluated the behaviour of 3a-androstanediol glucuronide (3a-diol G) in 170 women of whom 85 had polycystic ovary syndrome (PCOS), 35 had idiopathic hirsutism (IH) and 50 had regular cycles (control group). Of the women with PCOS, 45 were hirsute (PCOS-H) and 40 were non hirsute (PCOS-NH). Women in the control group were not hirsute. Hirsutism was assessed by the same physician using the Ferriman-Gallway score. The body mass index (BMI) was estimated in all of the women. Plasma concentrations of 3a-diol G were elevated only in hirsute patients, both with PCOS and with IH. Even in PCOS-NH, concentrations of 3a-diol G were higher compared with controls (P<0.001), but significantly lower (P < 0.001) than those of the PCOS-H and of the IH groups. The behaviour of 3a- diol G was not affected by BMI. European Journal of Endocrinology 138 421–424 Introduction The aim of our study is to provide further contri- butions to the clinical activity of 3a-diol G by evaluat- Hirsutism, affecting 5–8% of the whole female popula- ing its behaviour in a broad series of normal and tion of fertile age, is an important clinical and hyperandrogenic women, with and without hirsutism.
    [Show full text]