toxins Article Cyanobacterial Toxins and Peptides in Lake Vegoritis, Greece Sevasti-Kiriaki Zervou 1 , Kimon Moschandreou 2, Aikaterina Paraskevopoulou 1, Christophoros Christophoridis 1 , Elpida Grigoriadou 3, Triantafyllos Kaloudis 1, Theodoros M. Triantis 1 , Vasiliki Tsiaoussi 2 and Anastasia Hiskia 1,* 1 Laboratory of Photo-Catalytic Processes and Environmental Chemistry, Institute of Nanoscience & Nanotechnology, National Center for Scientific Research “Demokritos”, Patriarchou Grigoriou E & 27 Neapoleos Str, 15310 Agia Paraskevi, Athens, Greece;
[email protected] (S.-K.Z.);
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[email protected] (T.M.T.) 2 The Goulandris Natural History Museum—Greek Biotope/Wetland Centre, 14th km Thessaloniki-Mihaniona, Thermi P.O. Box 60394, 57001 Thessaloniki, Greece;
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[email protected] Abstract: Cyanotoxins (CTs) produced by cyanobacteria in surface freshwater are a major threat for public health and aquatic ecosystems. Cyanobacteria can also produce a wide variety of other understudied bioactive metabolites such as oligopeptides microginins (MGs), aeruginosins (AERs), aeruginosamides (AEGs) and anabaenopeptins (APs). This study reports on the co-occurrence of CTs and cyanopeptides (CPs) in Lake Vegoritis, Greece and presents their variant-specific profiles obtained during 3-years of monitoring (2018–2020). Fifteen CTs (cylindrospermopsin (CYN), anatoxin (ATX), nodularin (NOD), and 12 microcystins (MCs)) and ten CPs (3 APs, 4 MGs, 2 AERs and aeruginosamide Citation: Zervou, S.-K.; (AEG A)) were targeted using an extended and validated LC-MS/MS protocol for the simultaneous Moschandreou, K.; Paraskevopoulou, determination of multi-class CTs and CPs.