DOCSLIB.ORG

Narcolepsy and the Orexin Neuropeptide System

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Narcolepsy and the Orexin Neuropeptide System LESSONS FROM SLEEPY MICE: NARCOLEPSY AND THE OREXIN NEUROPEPTIDE SYSTEM APPROVED BY SUPERVISORY COMMITTEE DEDICATION I would like to thank my mentors and colleagues Masashi Yanagisawa, M.D., Ph.D.; Howard Gershenfeld, M.D.,Ph.D.; Michael Brown, M.D.; Rama Ranganathan, M.D., Ph.D.; Richard Chemelli, M.D.; Christopher Sinton, Ph.D.; Paul Boldt, Ph.D.; Thomas Kodadek, Ph.D.; and Glen R. Willie, M.D. I also thank my program directors and administrators Dennis McKearin, Ph.D.; Rodney Ulane, Ph.D.; Jean Wilson, M.D.; Robin Downing; Stephanie Robertson; Mark Lehrman, Ph.D.; and Carla Childers. I am especially thankful to my wife Abigail, my son Sam, and all my family for their love and support. LESSONS FROM SLEEPY MICE: NARCOLEPSY AND THE OREXIN NEUROPEPTIDE SYSTEM by JON TIMOTHY WILLIE DISSERTATION Presented to the Faculty of the Graduate School of Biomedical Sciences The University of Texas Southwestern Medical Center at Dallas In Partial Fulfillment of the Requirements For the Degree of DOCTOR OF PHILOSOPHY The University of Texas Southwestern Medical Center at Dallas Dallas, Texas June, 2005 Copyright by JON TIMOTHY WILLIE, 2005 All Rights Reserved LESSONS FROM SLEEPY MICE: NARCOLEPSY AND THE OREXIN NEUROPEPTIDE SYSTEM Publication No. JON TIMOTHY WILLIE, M.D., Ph.D. The University of Texas Southwestern Medical Center at Dallas, 2005 Supervising Professor: MASASHI YANAGISAWA, M.D., Ph.D. The hypothalamic orexin neuropeptide system is a neuronal pathway regulating behavioral vigilance states and metabolic functions. Orexins activate the orexin receptors type 1 and type 2 (OX2R). Melanin-concentrating hormone (MCH) is an anatomically- related peptide that may have complementary functions. Orexin null (orexin-/-) mice exhibit a behavioral and electroencephalographic phenotype similar to narcolepsy-cataplexy, a neurological disorder caused by orexin deficiency. Narcolepsy-cataplexy consists of inability to maintain wakefulness, abnormal intrusions of rapid-eye-movement (REM) sleep and related phenomena (i.e. cataplexy) into wakefulness, and poorly characterized metabolic v vi abnormalities. I demonstrate that both OX2R-/- and orexin-/- mice are unable to maintain wakefulness normally. In contrast, OX2R-/- mice are only mildly affected with abnormalities of REM sleep, whereas orexin-/- mice are severely affected. Thus, the profound dysregulation of REM sleep control unique to the narcolepsy-cataplexy syndrome emerges from loss of signaling through both OX2R-dependent and OX2R-independent pathways. Transgenic mice, in which orexin neurons are ablated, fail to respond normally to fasting with increased wakefulness and activity indicating that orexin neurons provide a crucial link between energy balance and arousal. Orexin-/- and MCH-/- mice have increased and decreased adiposity and susceptibility to diet-induced obesity, respectively. While orexin-/- mice exhibit sleepiness and cataplexy, MCH-/- mice are more wakeful than wild-type mice. Orexin-/-;MCH-/- exhibit exacerbation of the narcolepsy phenotype, indicating that orexin and MCH complementarily regulate behavioral stabilty. Mice that ectopically overexpress orexin in the brain exhibit reduced body weight and resistance to diet-induced obesity, inability to maintain sleep, and reduced REM sleep with abnormal myoclonic activity relative to wild-type controls, providing further evidence that orexin alters homeostatic set-points of both energy metabolism and sleep/wakefulnuss. Modafinil, a stimulant used to treat narcoleptics, effectively increases wakefulness but does not suppress cataplexy in orexin-/- mice, indicating that orexin is not required for the wake-promoting action of the drug. By contrast, expression of an orexin transgene in the brain completely prevented cataplectic arrests and other abnormalities of REM sleep in the absence of endogenous orexin neurons. Thus, orexin neuron-ablated mice retain the ability to respond to orexin neuropeptides and orexin receptor agonists would likely contribute to treating human narcolepsy. TABLE OF CONTENTS DEDICATION ....................................................................................................................... ii ABSTRACT ........................................................................................................................... v TABLE OF CONTENTS ..................................................................................................... vii PRIOR PUBLICATIONS ................................................................................................... viii LIST OF FIGURES .............................................................................................................. xi LIST OF TABLES ............................................................................................................. xvii LIST OF APPENDICES ................................................................................................... xviii LIST OF DEFINITIONS .................................................................................................... xix CHAPTER 1 - Introduction and Historical Perspective ......................................................... 1 CHAPTER 2 – Distinct Narcolepsy Syndromes in Orexin Receptor Type 2 (OX2R) and Orexin Knockout Mice: Molecular Genetics of Non-REM and REM Sleep Regulation 26 CHAPTER 3 - Coordinating Energy Balance and Arousal.................................................. 72 CHAPTER 4 - Genetic Interaction of Two Hypothalamic Peptides, Orexin and MCH ...... 91 CHAPTER 5 - Effects of Unregulated Orexin Overexpression ......................................... 133 CHAPTER 6 - Treatment of Narcolepsy – Modafinil........................................................ 154 CHAPTER 7 - Treatment of Narcolepsy - Genetic and Pharmacologic Rescue by Orexin 186 CHAPTER 8 - Conclusion.................................................................................................. 215 APPENDICES .................................................................................................................... 216 BIBLIOGRAPHY............................................................................................................... 255 VITAE ................................................................................................................................ 275 vii PRIOR PUBLICATIONS Original Reports Willie, J. T., Scammell, T. E., Renthal, W., Chemelli, R. M., Miller, M. S., Yanagisawa, M., Sinton, C. M.. Modafinil More Effectively Induces Wakefulness in Orexin Null Mice than in Wild-Type Littermates. Neuroscience. In press. Mieda, M., Willie, J. T., Hara, J., Sakurai, T., Yanagisawa, M. (2004). Orexin Peptides Prevent Cataplexy and Improve Wakefulness in an Orexin Neuron-Ablated Model of Narcolepsy in Mice. PNAS 101: 4649-54. Willie, J. T., Chemelli, R. M., Sinton, C. M., Tokita, S., Williams, S. C., Kisanuki, Y. Y., Marcus, J. N., Lee, C. E., Elmquist, J. K., Kohlmeier, K. A., Leonard, C. S., Richardson, J. A., Hammer, R. E., Yanagisawa, M. (2003). Distinct Narcolepsy Syndromes in Orexin Receptor Type-2 and Orexin Null Mice: Genetic Dissection of Non-REM and REM Sleep Regulatory Processes. Neuron 38: 715-30. Yamanaka, A., Beuckmann, C. T., Willie, J. T., Hara, J., Tsujino, N., Mieda, M., Tominaga, M., Yagami, K., Sugiyama, F., Goto, K., Yanagisawa, M., Sakurai, T. (2003). Hypothalamic Orexin Neurons Regulate Arousal According to Energy Balance in Mice. Neuron 38: 701-13. Georgescu, D., Zachariou, V., Barrot, M., Mieda, M., Willie, J. T., Eisch, A. J., Yanagisawa, M., Nestler, E. J., DiLeone, R. J. (2003). Involvement of the lateral hypothalamic peptide orexin in morphine dependence and withdrawal. Journal of Neuroscience 23: 3106-11. Chou, T. C., Lee, C. E., Lu, J., Elmquist, J. K., Hara, J., Willie, J. T., Beuckmann, C. T., Chemelli, R. M., Sakurai, T., Yanagisawa, M., Saper, C. B., Scammell, T. E. (2001). Orexin (hypocretin) neurons contain dynorphin. Journal of Neuroscience 21: RC168. Hara, J., Beuckmann, C. T., Nambu, T., Willie, J. T., Chemelli, R. M., Sinton, C. M., Sugiyama, F., Yanagi, K., Goto, K., Yanagisawa, M., and Sakurai, T. (2001). Genetic ablation of orexin neurons in mice results in narcolepsy, hypophagia and obesity. Neuron 30: 345-54. Chemelli, R. M., Willie, J. T., Sinton, C. M., Elmquist, J. K., Scammell, T., Richardson, J. A., Williams, S. C., Xiong, Y., Kisanuki, Y., Fitch, T. E., Nakazato, M., Hammer, M., Saper, C. B., and Yanagisawa, M. (1999). Narcolepsy in orexin knockout mice: molecular genetics of sleep regulation. Cell 98: 437-51. viii ix Vashee, S., Willie, J. T., Kodadek, T. (1998). Synergistic activation of transcription by physiologically unrelated transcription factors through cooperative DNA-binding. Biochemical & Biophysical Research Communications 247(2):530-5. Invited Reviews Willie, J. T. and Yanagisawa, M. Lessons from Sleepy Mice. In Narcolepsy and Hypersomnia. Ed. C. Bassetti et al. Marcel Dekker, Inc. In press. Willie, J. T., Chemelli, R. M., Sinton, C. M., and Yanagisawa, M. (2001). To eat or sleep? Orexin in the regulation of feeding and wakefulness. Annual Review of Neuroscience 24:429-58. Peer-Reviewed, Published Abstracts Willie, J. T., Sinton, C. M., Mieda, M., Maratos-Flier, E., Yanagisawa, M. (2003). Orexin and melanin-concentrating hormone (MCH) double knockout mice: Compensatory role for MCH in narcolepsy-cataplexy. Sleep (Abstr). 26:A50. Willie, J. T., Beuckmann, C. T., Hara, J., Sakurai, T., Yanagisawa, M. ( 2002). Ectopic Expression of an Orexin Transgene Rescues
Load more

Recommended publications
  • 826 Original Article Postprandial Somnolence and Its Awareness
    Bangladesh Journal of Medical Science Vol. 20 No. 04 October’21 Original article Postprandial Somnolence and its awareness among the Medical Undergraduate Students: A cross- sectional study Abhishek Chaturvedi1, Anitha Guru2, Naveen Kumar3, Ling Yi Lin4, Daniel YeapTze Wei5, Lai Kah Sheng6, Leow Hjun Yee7 Abstract Introduction: Postprandial somnolence or commonly referred to as food coma is generally experienced after the ingestion of afternoon meals. The performance of an individual gets affected after the ingestion of a heavy meal and this is more pertinent in a college setup where students have to attend a lecture right after the meal. The objective of this study was to assess the awareness of medical students about the factors responsible for postprandial somnolence, to identify the methods used to counteract it and to ascertain lecturers’ perception on responsiveness and participation of the students in a post lunch lecture. Methods: Total 330 students (first year to third year MBBS students) aged between 18-21 years and 40 lecturers teaching first and second year MBBS students were involved in this study. Two separate questionnaires (Part A: students’ perception, and B: lecturers’ perception) were prepared, peer-reviewed, validated and administered to the respective participants. All the responses were compiled and expressed in frequency percentage. Statistical analysis was performed using Statistical Package for Social Sciences, version 15.0 for a level of statistical significance of 5%. Pearson correlation was used to get the association between the variables. Results: About 55.75% students were aware about the role of serotonin and melatonin in drowsiness but 45.75% students did not know that food rich in tryptophan relaxes the brain and results in sleepiness.
    [Show full text]
  • Descriptive Psychopathology of the Acute Effects of Intravenous Delta-9-Tetrahydrocannabinol Administration in Humans
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by King's Research Portal King’s Research Portal DOI: 10.3390/brainsci9040093 Document Version Publisher's PDF, also known as Version of record Link to publication record in King's Research Portal Citation for published version (APA): Colizzi, M., Weltens, N., McGuire, P., Van Oudenhove, L., & Bhattacharyya, S. (2019). Descriptive psychopathology of the acute effects of intravenous delta-9-tetrahydrocannabinol administration in humans. Brain Sciences, 9(4), [93]. https://doi.org/10.3390/brainsci9040093 Citing this paper Please note that where the full-text provided on King's Research Portal is the Author Accepted Manuscript or Post-Print version this may differ from the final Published version. If citing, it is advised that you check and use the publisher's definitive version for pagination, volume/issue, and date of publication details. And where the final published version is provided on the Research Portal, if citing you are again advised to check the publisher's website for any subsequent corrections. General rights Copyright and moral rights for the publications made accessible in the Research Portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognize and abide by the legal requirements associated with these rights. •Users may download and print one copy of any publication from the Research Portal for the purpose of private study or research. •You may not further distribute the material or use it for any profit-making activity or commercial gain •You may freely distribute the URL identifying the publication in the Research Portal Take down policy If you believe that this document breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim.
    [Show full text]
  • Hengist, A., Edinburgh, R. M., Davies, R. G., Walhin, J-P., Buniam, J., James, L
    Hengist, A., Edinburgh, R. M., Davies, R. G., Walhin, J-P., Buniam, J., James, L. J., Rogers, P. J., Gonzalez, J. T., & Betts, J. A. (2020). The physiological responses to maximal eating in men. British Journal of Nutrition, 1-32. https://doi.org/10.1017/S0007114520001270 Peer reviewed version Link to published version (if available): 10.1017/S0007114520001270 Link to publication record in Explore Bristol Research PDF-document This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Cambridge University Press at https://www.cambridge.org/core/journals/british-journal-of- nutrition/article/physiological-responses-to-maximal-eating-in-men/25C29D75CB1553B9D3D23E276295A4D8 . Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ Downloaded from Accepted manuscript https://www.cambridge.org/core The physiological responses to maximal eating in men Aaron Hengist1, Robert M. Edinburgh1, Russell G. Davies1, Jean-Philippe Walhin1, Jariya Buniam1,2, Lewis J. James3, Peter J. Rogers4,5, Javier T. Gonzalez1, James A. Betts1* . IP address: 86.31.142.105 1Department for Health, University of Bath, BA2 7AY , on 2Department of Physiology, Faculty of Science, Mahidol University, Bangkok,
    [Show full text]
  • Music and Its Effect on Typing Speed for Clerical Workers During Postprandial Somnolence
    MUSIC AND ITS EFFECT ON TYPING SPEED FOR CLERICAL WORKERS DURING POSTPRANDIAL SOMNOLENCE LIN YU TONG CULTURAL CENTRE UNIVERSITY OF MALAYA KUALA LUMPUR University of Malaya 2016 MUSIC AND ITS EFFECT ON TYPING SPEED FOR CLERICAL WORKER DURING POSTPRANDIAL SOMNOLENCE STATE LIN YU TONG DESSERTATION SUBMITTED IN FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER IN PERFORMING ARTS CULTURAL CENTRE UNIVERSITY OF MALAYA KUALA LUMPUR University2016 of Malaya UNIVERSITY OF MALAYA ORIGINAL LITERARY WORK DECLARATION Name of Candidate: Lin Yu Tong Registration/Matric No: RGI140012 Name of Degree: Master of Performing Arts (Music) Title of Project Paper/Research Report/Dissertation/Thesis (“this Work”): Music and Effect on Typing Speed For Clerical Worker During Postprandial Somnolence State Field of Study: Musicology I do solemnly and sincerely declare that: (1) I am the sole author/writer of this Work; (2) This Work is original; (3) Any use of any work in which copyright exists was done by way of fair dealing and for permitted purposes and any excerpt or extract from, or reference to or reproduction of any copyright work has been disclosed expressly and sufficiently and the title of the Work and its authorship have been acknowledged in this Work; (4) I do not have any actual knowledge nor do I ought reasonably to know that the making of this work constitutes an infringement of any copyright work; (5) I hereby assign all and every rights in the copyright to this Work to the University of Malaya (“UM”), who henceforth shall be owner of the copyright in this Work and that any reproduction or use in any form or by any means whatsoever is prohibited without the written consent of UM having been first had and obtained; (6) I am fully aware that if in the course of making this Work I have infringed any copyright whether intentionally or otherwise, I may be subject to legal action or any other action as may be determined by UM.
    [Show full text]
  • An Assessment Guide Kingman P. Strohl Case Western Reserve University Cleveland , OH , USA
    Kingman P. Strohl Competencies in Sleep Medicine An Assessment Guide Competencies in Sleep Medicine Kingman P. Strohl Competencies in Sleep Medicine An Assessment Guide Kingman P. Strohl Case Western Reserve University Cleveland , OH , USA ISBN 978-1-4614-9064-7 ISBN 978-1-4614-9065-4 (eBook) DOI 10.1007/978-1-4614-9065-4 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2013956618 © Springer Science+Business Media New York 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifi cally for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.
    [Show full text]
  • Feeding Habits and Its Impact on Concentration and Attentiveness
    Research Article iMedPub Journals ARCHIVES OF MEDICINE 2016 http://www.imedpub.com/ Vol.8 No.5:9 ISSN 1989-5216 DOI: 10.21767/1989-5216.1000167 Feeding Habits and its Impact on Concentration and Attentiveness among Medical Students in Dominica Alice Solomon1, Eucharia Mbat1, Srinivas Medavarapu3*, Olajumoke Faleti1 and David Otohinoyi2 1Department of Basic Sciences, All Saints University School of Medicine, Roseau, Dominica 2Department of Clinical Sciences, All Saints University School of Medicine, Roseau, Dominica 3All Saints University School of Medicine, Roseau, Dominica *Corresponding author: Srinivas Medavarapu, Assistant Professor, All Saints University School of Medicine, Roseau, Dominica, Tel: 18483915130; E- mail: [email protected] Received date: August 30, 2016; Accepted date: October 13, 2016; Published date: October 20, 2016 Citation: Solomon A, Mbat E, Medavarapu S, Faleti O, Otohinoyi D. Feeding Habits and its Impact on Concentration and Attentiveness among Medical Students in Dominica. Arch Med. 2016, 8:5 Copyright: © 2016 Solomon A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. broken down into simple sugars [2]. The brain needs at least 130 grams of carbohydrates (about 520 calories per day) to function Abstract adequately [1,2]. It is usually recommended that 45-65% of total daily calories should come from carbohydrates, 12-20% from Title: Feeding habits and its impact on concentration and proteins, and 20-35% from fats [1]. attentiveness among medical students in Dominica. Food fortifies the body with energy, vitamins, and minerals Background: Food is essential for good health.
    [Show full text]
  • Buckleyrusselljphd2021.Pdf (8.065Mb)
    Is Brain Function Impaired in Moderate Sleep Apnoea: Before and After Six Months of APAP. by Russ J. Buckley A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy (Medicine) in the University of Otago, Christchurch, New Zealand July 2021 Abstract The physiological impact of severe levels of obstructive sleep apnoea and the benefits of treatment are well documented. Yet more of the population suffer sleep apnoea of moderate severity. However, we still have substantial gaps in our understanding on the physiological impact and treatment of people with sleep apnoea of moderate severity. Our study addressed whether moderate obstructive sleep apnoea (OSA) had an impact on cerebral blood flow, cognition, or microsleeps. It also explored the effect of 6–7 months of auto-titrating positive airway pressure (APAP) therapy on physiological measurements and quality-of-life in persons with moderate OSA. Twenty-four patients aged 32–79 years (median age 59, 10 female), who had previously presented to the Christchurch Hospital Sleep Unit and subsequently diagnosed with sleep apnoea of moderate severity consented to our study. Following a Level II polysomnography to confirm their suitability to participate, thirteen of these participants, aged 43–79 years (median age 59, 4 female), were placed on APAP therapy, with instructions to use it for at least 4 hr per night for 70% or more of the nights. The remaining 11 participants aged 32–79 years (median age 62, 6 female), were placed in an untreated cohort. Placement in the treated cohort was dependent on order of presentation with those first recruited offered a place in the treated cohort.
    [Show full text]
  • Insula and Its Dual Function in Sleep and Wakefulness
    ORIGINAL RESEARCH published: 11 February 2021 doi: 10.3389/fnsys.2020.592660 The Mysterious Island: Insula and Its Dual Function in Sleep and Wakefulness Ekaterina V. Levichkina 1,2, Irina I. Busygina 3, Marina L. Pigareva 4 and Ivan N. Pigarev 1* 1 Institute for Information Transmission Problems (Kharkevich Institute), Russian Academy of Sciences, Moscow, Russia, 2 Department of Optometry and Vision Sciences, The University of Melbourne, Parkville, VIC, Australia, 3 Pavlov Institute of Physiology, Russian Academy of Sciences, Saint Petersburg, Russia, 4 Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia In the recent sleep studies, it was shown that afferentation of many cortical areas switches during sleep to the interoceptive one. However, it was unclear whether the insular cortex, which is often considered as the main cortical visceral representation, maintains the same effective connectivity in both states of vigilance, or processes interoceptive information predominantly in one state. We investigated neuronal responses of the cat insular cortex to electrical stimulations of the intestinal wall delivered during wakefulness and natural sleep. Marked increase was observed in the number of insular neurons responding to this stimulation in sleep comparing to wakefulness, and enlarged amplitudes of evoked local field potentials were found as well. Moreover, most of the cells responding to intestinal stimulation in wakefulness never responded to identical stimuli during sleep and vice versa. It was also shown that applied low intensity intestinal stimulations had never compromised sleep quality. In addition, experiments with microstimulation of the insular cortex and recording of intestinal myoelectric activity demonstrated that effective Edited by: James W.
    [Show full text]
  • Effects of Dissolved Potassium on Growth Performance, Body Composition, and Welfare of Juvenile African Catfish (Clarias Gariepinus)
    fishes Article Effects of Dissolved Potassium on Growth Performance, Body Composition, and Welfare of Juvenile African Catfish (Clarias gariepinus) Lisa Carolina Wenzel 1,*,†, Sebastian Marcus Strauch 1,*,† , Ep Eding 2, Francisco Xose Presas-Basalo 1,2, Berit Wasenitz 1 and Harry Wilhelm Palm 1 1 Professorship Aquaculture and Sea-Ranching, Faculty of Agricultural and Environmental Sciences, University of Rostock, Justus-von-Liebig-Weg 6, 18059 Rostock, Germany; [email protected] (F.X.P.-B.); [email protected] (B.W.); [email protected] (H.W.P.) 2 Aquaculture & Fisheries, Wageningen University and Research, Droevendaalsesteeg 2, 6708 PB Wageningen, The Netherlands; [email protected] * Correspondence: [email protected] (L.C.W.); [email protected] (S.M.S.) † These authors contributed equally to this study. Abstract: Optimal crop production in aquaponics is influenced by water pH and potassium concen- trations. The addition of potassium hydroxide (KOH) into the recirculating aquaculture system (RAS) may benefit aquaponics by increasing the water pH for better biofilter activity and supplementing K for better plant growth and quality. We investigated the growth, feed conversion, body composition and welfare indicators of juvenile African catfish (Clarias gariepinus) treated with four concentrations −1 Citation: Wenzel, L.C.; Strauch, S.M.; of K (K0 = 2, K200 = 218, K400 = 418, and K600 = 671 mg L ). While growth, feed conversion Eding, E.; Presas-Basalo, F.X.; and final body composition were unaffected, the feeding time and individual resting significantly Wasenitz, B.; Palm, H.W. Effects of increased with increasing K+. The swimming activity and agonistic behavior were reduced signifi- Dissolved Potassium on Growth cantly under increased concentrations of K+.
    [Show full text]
  • Hypothalamic Orexin Neurons Regulate Arousal According to Energy Balance in Mice
    Neuron, Vol. 38, 701–713, June 5, 2003, Copyright 2003 by Cell Press Hypothalamic Orexin Neurons Regulate Arousal According to Energy Balance in Mice Akihiro Yamanaka,1,2,5,7 Carsten T. Beuckmann,6,7 The molecular and physiological basis of this evolution- Jon T. Willie,6,7 Junko Hara,1,2,5 Natsuko Tsujino,1,2,5 arily conserved phenomenon remains poorly under- Michihiro Mieda,6 Makoto Tominaga,1,3 stood. Ken-ichi Yagami,1,4 Fumihiro Sugiyama,1,4 Orexins, also called hypocretins, are a pair of neuro- Katsutoshi Goto,1,2 Masashi Yanagisawa,1,5,6,* peptides expressed by a specific population of neurons and Takeshi Sakurai1,2,5,* in the lateral hypothalamic area (LHA), a region of the 1Institute of Basic Medical Science brain implicated in feeding, arousal, and motivated be- 2 Department of Pharmacology havior (de Lecea et al., 1998; Sakurai et al., 1998; Chemelli 3 Department of Molecular Neurobiology et al., 1999; Willie et al., 2001). Orexin-A (hypocretin-1) 4 Laboratory Animal Research Center and orexin-B (hypocretin-2) are derived from a common University of Tsukuba precursor peptide, the product of the prepro-orexin Ibaraki 305-8575 gene (Sakurai et al., 1998, 1999). The actions of orexins Japan are mediated by two G protein-coupled receptors 5 ERATO Yanagisawa Orphan Receptor Project termed orexin receptor type 1 and orexin receptor type 2 Japan Science and Technology Corporation (Sakurai et al., 1998). Orexin-containing neurons project Tokyo 135-0064 from the LHA to numerous brain regions, with the limbic Japan system, hypothalamus, and monoaminergic and cholin- 6 Howard Hughes Medical Institute ergic nuclei of brainstem receiving particularly strong Department of Molecular Genetics innervations (Peyron et al., 1998; Date et al., 1999; University of Texas Southwestern Medical Center Nambu et al., 1999).
    [Show full text]
  • Meet Sign– up Procedures
    Volume 1, Edition 11 November 2014 Published by Lone Star Swim Team, 9597 Jones Road, PMB 197, Houston TX 77065 Lone Star swimmers had a great meet. We had lots of UPCOMING MEETS: personal best times that were achieved. October meet marked LSST’s best showing with over 50% 11/15-16: Gulf Open of swimmers participating in the meet with many being Meet—LSST first-timers. 12/3-6: AT&T SC National Highlight Swim: Heat 2 of the Men’s 500FREE Payton Silburn Championships @ Greensboro, NC dropped over 23 seconds even though Facility Lights accidentally turned completely off during the swim! 12/5-7: Gulf Senior Full Results posted on Gulf’s website at www.gulfswimming.org Champs—TWST 12/5-7: Gulf Fall Champs—LSST MEET SIGN– UP PROCEDURES 12/12-14: Gulf Age Group 1. Receive Meet Invitation Email from either Coach Eddie or Coach Judy. Champs—AGS 2. Use the link provided in the email to log on to LSST’s website. 3. Make sure you are logged in. 12/13: 8 & Under 4. Click the “Edit Commitment” button for the proper Event. Champs—T B D 5. Select the swimmer you want to enter in the meet by clicking on their name. 6. Read over the Entry Rules at top for important information and to know how many individual events per day you can choose for your swimmer. 7. Drop down the menu under “Declaration” and choose “YES, Please sign [name] up for this event. SAVE THE DATE: 8. Click the box before each event to select the events - do not exceed the maximum number of individual events listed in the Rules 12/20: Annual Christmas HINT: Look for Day and Session numbers to know when your swimmer’s events Party will be swum and to choose correct number of events on each day.
    [Show full text]
  • Helps Correct Anxiety & Poor Sleep Tryptophan
    Tryptophan (Amino Acid) Helps Correct Anxiety & Poor Sleep Chlorella Algae (RECOVERYbits) has the highest concentration of TRYPTOPHAN of any Food The body uses tryptophan to help make serotonin, the neurotransmitter that produces healthy sleep and a stable mood. Chlorella algae has the highest concentration of tryptophan in the world and is the most natural way to achieve a healthy body, happy mind and good sleep. Aging is often accompanied by a spectrum of mood disorders that include irritability, stress, and anxiety. These symptoms, along with more severe ones like sleep disorders, depression, aggressive behavior, reduced motivation, and suicidal thinking have all been traced to depletion of brain levels of serotonin, a neurotransmitter that has been called the “happiness hormone.” With the progression of age, chronic, low-grade inflammation sets the stage for degenerative disease in almost every area of the body. While this inflammation often leads to diabetes, cancer, and heart disease, it also affects the brain by interfering with the production and release of serotonin. In order for your body to manufacture serotonin, it needs a sufficient supply of the amino acid, tryptophan. Much of what is now known about the role of serotonin in psychiatric and behavioral disturbances comes from studies of tryptophan depletion. Lowering tryptophan levels triggers a corresponding drop in brain serotonin production and can impact mood, impair memory, and increase aggression. Although you can’t supplement with serotonin itself, tryptophan is found in protein and the highest concentration of tryptophan is found in algae. Chlorella algae has five times more tryptophan than turkey – the food that most people associate with tryptophan and sleepiness.
    [Show full text]