140-004-488.02 1. The transient expression of key developmental regulators, regulators, developmental key of expression transient The 1.2 1.1 1. Description vitro

Contents 2. Components validated to ensure high level expression after transfection. after expression level high ensure to validated CCAAT/enhancer-binding beta (C/EBP-beta) is a member (C/EBP-beta) a member is beta protein CCAAT/enhancer-binding Quality controlQuality Formulation In pluripotent stem cell research overexpression of C/EBP-beta has has of C/EBP-beta overexpression research cell stem pluripotent In early the during induced transiently is C/EBP-beta addition, In cooperation with other adipogenic factors such as PPAR-gamma. PPAR-gamma. as such factors adipogenic other with cooperation C/EBP- as such members, family C/EBP other with complexes of transcription family containing (bZIP) zipper leucine basic of the recombinases or markers via mRNA transfection is a powerful tool tool apowerful is transfection mRNA via or markers recombinases that are important for immune and inflammatory responses. responses. inflammatory and for immune important are that genes factors. It can function as a homodimer or in heterodimeric heterodimeric or in ahomodimer as function It can factors. pure, highly are mRNAs StemMACS fate. cell for modulating macsde stages of adipocyte differentiation and promotes in adipogenesis and promotes differentiation of adipocyte stages www.miltenyibiotec.com www.miltenyibiotec.com alpha, -beta or -gamma. C/EBP-beta plays a role in the regulation of regulation the arole in plays C/EBP-beta or -gamma. -beta alpha, Storage Specifications been used to induce the differentiation into adipose tissue. intoadipose differentiation the induce to used been Phone Phone Friedrich-Ebert-Straße Friedrich-Ebert-Straße Miltenyi Biotec B.V. KG &Co. Biotec Miltenyi Description Background information Background Principle Applications Applications 1.2 1.2 1.1 Principle Protocol: Dissolving of lyophilizate Dissolving Protocol: -transcribed mRNAs that have been carefully optimized and and optimized carefully have been that mRNAs -transcribed +49 2204 8306-0, 2204 +49 @

miltenyi.com Background information Background

–70°C for up3month. to Store the lyophilized product at –20 °C. The The at –20 °C. product lyophilized Store the vitro In Water, Double-distilled 1 mL Lyophilized from a filtered (0.2 µm)solution. (0.2 afiltered from Lyophilized mRNA size has been verified on an Agilent Agilent an on verified been has size mRNA 20 RNA. expiration date is indicated on the label. After After label. on the indicated is date expiration with modified been has that mRNA capped Bioanalyzer System. Cebpb protein expression expression protein Cebpb System. Bioanalyzer 1051). ID TCF5; LAP, CRP2, reconstitution, the product can be stored at stored be can product the reconstitution, after transfection was confirmed by confirmed was transfection after the innate antiviral response to single-stranded single-stranded to response antiviral innate the reduce to 5-methyl-cytidine and pseudouridine (CEBPB, C/EBP-beta factor transcription the immunofluorescence. 68, 5142968, Fax Fax µg StemMACS™ Cebpb mRNA mRNA Cebpb StemMACS™ µg +49 2204 85197 2204 +49 Bergisch Gladbach, Germany Bergisch Gladbach, transcribed, polyadenylated and polyadenylated and transcribed, RNase-free encoding encoding in in ▲ 1. Applications 1.3 2. Wear gloves, use RNase-free reagents, tubes, and pipette tips. pipette and tubes, reagents, RNase-free use Wear gloves, 3. mRNA Cebpb StemMACS™ 2. ● Miltenyi Biotec provides technical support worldwide. Visit worldwide. support technical provides Biotec Miltenyi www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec Biotec Miltenyi nearest your find to www.miltenyibiotec.com/local For satisfactory transfection results, use a protocol that is is that aprotocol use results, transfection For satisfactory Refer to to Refer or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of evaluation easy allow mRNA eGFP Nuclear or StemMACS™ mRNA eGFP StemMACS™ type. cell specific for your optimized contact. transfection efficiency and are recommended as positive controls. positive as recommended are and efficiency transfection page 1/2

RNA is susceptible to degradation by exogenous ribonucleases. ribonucleases. by exogenous degradation to susceptible is RNA Protocol: Reconstitution of lyophilizate of Reconstitution Protocol: Prepare aliquots and store at –70 store and aliquots Prepare the of bottom the contentthe at collect to centrifuge Briefly Differentiation of pluripotent stem cells into adipose tissue. intoadipose cells stem of pluripotent Differentiation Dissolve StemMACS Cebpb mRNA in 200 µL of Double- µL 200 in mRNA Cebpb StemMACS Dissolve distilled Water. The final concentration Vortexwill thoroughly. distilled aliquots to more than two freeze-thaw cycles. cycles. freeze-thaw two more to than aliquots tube. be 0.1 be www.miltenyibiotec.com µg/µL. for all data sheets and protocols. protocols. and sheets data for all

–80 to °C Order no. 130-104-377 °C. Do not subject not subject Do °C.

Order No. 130-104-377

Legal notices Limited product warranty Miltenyi Biotec B.V. & Co. KG and/or its affiliate(s) warrant this product to be free from material defects in workmanship and materials and to conform substantially with Miltenyi Biotec’s published specifications for the product at the time of order, under normal use and conditions in accordance with its applicable documentation, for a period beginning on the date of delivery of the product by Miltenyi Biotec or its authorized distributor and ending on the expiration date of the product’s applicable shelf life stated on the product label, packaging or documentation (as applicable) or, in the absence thereof, ONE (1) YEAR from date of delivery (“Product Warranty”). Miltenyi Biotec’s Product Warranty is provided subject to the warranty terms as set forth in Miltenyi Biotec’s General Terms and Conditions for the Sale of Products and Services available on Miltenyi Biotec’s website at www.miltenyibiotec.com, as in effect at the time of order (“Product Warranty”). Additional terms may apply. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS. THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING IF A PRODUCT IS SUITABLE FOR CUSTOMER’S PARTICULAR PURPOSE AND APPLICATION METHODS.

Technical information The technical information, data, protocols, and other statements provided by Miltenyi Biotec in this document are based on information, tests, or experience which Miltenyi Biotec believes to be reliable, but the accuracy or completeness of such information is not guaranteed. Such technical information and data are intended for persons with knowledge and technical skills sufficient to assess and apply their own informed judgment to the information. Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein. All information and specifications are subject to change without prior notice. Please contact Miltenyi Biotec Technical Support or visit www.miltenyibiotec.com for the most up-to-date information on Miltenyi Biotec products.

Licenses This product and/or its use may be covered by one or more pending or issued patents and/or may have certain limitations. Certain uses may be excluded by separate terms and conditions. Please contact your local Miltenyi Biotec representative or visit Miltenyi Biotec’s website at www.miltenyibiotec.com for more information. The purchase of this product conveys to the customer the non-transferable right to use the purchased amount of the product in research conducted by the customer (whether the customer is an academic or for-profit entity). This product may not be further sold. Additional terms and conditions (including the terms of a Limited Use Label License) may apply. CUSTOMER’S USE OF THIS PRODUCT MAY REQUIRE ADDITIONAL LICENSES DEPENDING ON THE SPECIFIC APPLICATION. THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING FOR ITSELF WHETHER IT HAS ALL APPROPRIATE LICENSES IN PLACE. Miltenyi Biotec provides no warranty that customer’s use of this product does not and will not infringe intellectual property rights owned by a third party. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.

Trademarks The Miltenyi Biotec logo and StemMACS are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.

Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.

Unless otherwise specifically indicated, all Miltenyi Biotec products and services page 2/2 are for research use only and not for diagnostic or therapeutic use. 140-004-488.02