Effect of Nitrogen and Potash on Top Borer
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www.nature.com/scientificreports OPEN Towards a global DNA barcode reference library for quarantine identifcations of lepidopteran Received: 28 November 2018 Accepted: 5 April 2019 stemborers, with an emphasis on Published: xx xx xxxx sugarcane pests Timothy R. C. Lee 1, Stacey J. Anderson2, Lucy T. T. Tran-Nguyen3, Nader Sallam4, Bruno P. Le Ru5,6, Desmond Conlong7,8, Kevin Powell 9, Andrew Ward10 & Andrew Mitchell1 Lepidopteran stemborers are among the most damaging agricultural pests worldwide, able to reduce crop yields by up to 40%. Sugarcane is the world’s most prolifc crop, and several stemborer species from the families Noctuidae, Tortricidae, Crambidae and Pyralidae attack sugarcane. Australia is currently free of the most damaging stemborers, but biosecurity eforts are hampered by the difculty in morphologically distinguishing stemborer species. Here we assess the utility of DNA barcoding in identifying stemborer pest species. We review the current state of the COI barcode sequence library for sugarcane stemborers, assembling a dataset of 1297 sequences from 64 species. Sequences were from specimens collected and identifed in this study, downloaded from BOLD or requested from other authors. We performed species delimitation analyses to assess species diversity and the efectiveness of barcoding in this group. Seven species exhibited <0.03 K2P interspecifc diversity, indicating that diagnostic barcoding will work well in most of the studied taxa. We identifed 24 instances of identifcation errors in the online database, which has hampered unambiguous stemborer identifcation using barcodes. Instances of very high within-species diversity indicate that nuclear markers (e.g. 18S, 28S) and additional morphological data (genitalia dissection of all lineages) are needed to confrm species boundaries. -
RNA-Seq of Rice Yellow Stem Borer Scirpophaga Incertulas Reveals Molecular Insights During Four Larval Developmental Stages
INVESTIGATION RNA-seq of Rice Yellow Stem Borer Scirpophaga incertulas Reveals Molecular Insights During Four Larval Developmental Stages Pichili Renuka,* Maganti S. Madhav,*,1 Ayyagari Phani Padmakumari,† Kalyani M. Barbadikar,* Satendra K. Mangrauthia,* Kola Vijaya Sudhakara Rao,* Soma S. Marla,‡ and Vemuri Ravindra Babu§ † § *Department of Biotechnology, Department of Entomology, and Department of Plant Breeding, ICAR-Indian Institute of Rice Research, Hyderabad, Telangana 500030, India and ‡Division of Genomic Resources, ICAR-National Bureau of Plant Genomic Resources, New Delhi, India ABSTRACT The yellow stem borer (YSB), Scirpophaga incertulas, is a prominent pest in rice cultivation KEYWORDS causing serious yield losses. The larval stage is an important stage in YSB, responsible for maximum in- insect festation. However, limited knowledge exists on the biology and mechanisms underlying the growth and Scirpophaga differentiation of YSB. To understand and identify the genes involved in YSB development and infestation, incertulas so as to design pest control strategies, we performed de novo transcriptome analysis at the first, third, fifth, de novo and seventh larval developmental stages employing Illumina Hi-seq. High-quality reads (HQR) of 229 Mb transcriptome were assembled into 24,775 transcripts with an average size of 1485 bp. Genes associated with various RNAi metabolic processes, i.e., detoxification mechanism [CYP450, GSTs, and carboxylesterases (CarEs)], RNA growth and interference (RNAi) machinery (Dcr-1, Dcr-2, Ago-1, Ago-2, Sid-1, Sid-2, Sid-3, and Sid-1-related gene), development chemoreception (CSPs, GRs, OBPs, and ORs), and regulators [transcription factors (TFs) and hormones] were detoxification differentially regulated during the developmental stages. Identification of stage-specific transcripts made it mechanism possible to determine the essential processes of larval development. -
Pathogen of Chilo Infliscatelllls Snellen P.SIVASANKARAN, S.EASWARAMOORTHY and H.DAVID Sugarcane Breeding Institute Coimbatore - 641 007
J.Biol.Control, 4 (I), 48-51, 1990 Pathogenicity and Host Range of Beauveria nr. bassiana, a Fungal Pathogen of Chilo infliscatelllls Snellen P.SIVASANKARAN, S.EASWARAMOORTHY and H.DAVID Sugarcane Breeding Institute Coimbatore - 641 007 ABSTRACT Second and third instar larvae of the shoot borer Chilo infuseatellus Snell., were more susceptible (51.47 to 65.2%) to the fungus, Beauveria nr. bassiana even at a low dosage (105 or 106 spores/ml). At 107 spores/ml, the mortality observed was 68.53-75.93 %. Mortality of the larvae decreased with increase In larval age or decrease in the concentration of tbe fungus. The fungus took less time to cause mortality in second Instar larvae and the incubation period Increased with increase in the age of the larvae or decrease in the dosage. All the six species of phytophagous insects tested Viz., Chilo sacehariphagus in die us (Kapur), C. partellus Swinhoc, SCirpophaga exeerptalis Walker, Sesamia inferens Walker, Hello/his arnilgera Hubner and Spodoptera litura Fabricius were susceptible to the fungal infection. The mean mortality of third Instar larvae varied from 50.0 to 65.0 per cent in the different species. The fungus was not infective to Sturmiopsls inferens Tns. tbe principal larval parasite of the shoot borer. Key Words: Beauveria nr. bassiana, Chilo inJuscare/lus, pathogenicity, host range The shoot borer Chilo infuscatellus Snellen sporulated abundantly. Fully sporulated fungal is cosmopolitan in distribution (Avasthy and mat along with carrot pieces was taken out. Tiwari. 1986) and poscs serious problems to thoroughly washed in water, filtered and used cane cultivation under drought and hot summer at thc required spore concentration. -
Crambidae Biosecurity Occurrence Background Subfamilies Short Description Diagnosis
Diaphania nitidalis Chilo infuscatellus Crambidae Webworms, Grass Moths, Shoot Borers Biosecurity BIOSECURITY ALERT This Family is of Biosecurity Concern Occurrence This family occurs in Australia. Background The Crambidae is a large, diverse and ubiquitous family of moths that currently comprises 11,500 species globally, with at least half that number again undescribed. The Crambidae and the Pyralidae constitute the superfamily Pyraloidea. Crambid larvae are concealed feeders with a great diversity in feeding habits, shelter building and hosts, such as: leaf rollers, shoot borers, grass borers, leaf webbers, moss feeders, root feeders that shelter in soil tunnels, and solely aquatic life habits. Many species are economically important pests in crops and stored food products. Subfamilies Until recently, the Crambidae was treated as a subfamily under the Pyralidae (snout moths or grass moths). Now they form the superfamily Pyraloidea with the Pyralidae. The Crambidae currently consists of the following 14 subfamilies: Acentropinae Crambinae Cybalomiinae Glaphyriinae Heliothelinae Lathrotelinae Linostinae Midilinae Musotiminae Odontiinae Pyraustinae Schoenobiinae Scopariinae Spilomelinae Short Description Crambid caterpillars are generally cylindrical, with a semiprognathous head and only primary setae (Fig 1). They are often plainly coloured (Fig. 16, Fig. 19), but can be patterned with longitudinal stripes and pinacula that may give them a spotted appearance (Fig. 10, Fig. 11, Fig. 14, Fig. 22). Prolegs may be reduced in borers (Fig. 16). More detailed descriptions are provided below. This factsheet presents, firstly, diagnostic features for the Pyraloidea (Pyralidae and Crambidae) and then the Crambidae. Information and diagnostic features are then provided for crambids listed as priority biosecurity threats for northern Australia. -
CURRICULUM VITAE Matthew P. Ayres
CURRICULUM VITAE Matthew P. Ayres Department of Biological Sciences, Dartmouth College, Hanover, NH 03755 (603) 646-2788, [email protected], http://www.dartmouth.edu/~mpayres APPOINTMENTS Professor of Biological Sciences, Dartmouth College, 2008 - Associate Director, Institute of Arctic Studies, Dartmouth College, 2014 - Associate Professor of Biological Sciences, Dartmouth College, 2000-2008 Assistant Professor of Biological Sciences, Dartmouth College, 1993 to 2000 Research Entomologist, USDA Forest Service, Research Entomologist, 1993 EDUCATION 1991 Ph.D. Entomology, Michigan State University 1986 Fulbright Fellowship, University of Turku, Finland 1985 M.S. Biology, University of Alaska Fairbanks 1983 B.S. Biology, University of Alaska Fairbanks PROFESSIONAL AFFILIATIONS Ecological Society of America Entomological Society of America PROFESSIONAL SERVICES Member, Board of Editors: Ecological Applications; Member, Editorial Board, Population Ecology Referee: (10-15 manuscripts / year) American Naturalist, Annales Zoologici Fennici, Bioscience, Canadian Entomologist, Canadian Journal of Botany, Canadian Journal of Forest Research, Climatic Change, Ecography, Ecology, Ecology Letters, Ecological Entomology, Ecological Modeling, Ecoscience, Environmental Entomology, Environmental & Experimental Botany, European Journal of Entomology, Field Crops Research, Forest Science, Functional Ecology, Global Change Biology, Journal of Applied Ecology, Journal of Biogeography, Journal of Geophysical Research - Biogeosciences, Journal of Economic -
Science and the Sustainable Intensification of Global Agriculture
Reaping the benefits Science and the sustainable intensification of global agriculture October 2009 Cover image: From an illustration of a push-pull system for pest control, courtesy of The Gatsby Charitable Foundation. The Quiet Revolution: Push-Pull Technology and the African Farmer. Gatsby Charitable Foundation 2005. Reaping the benefi ts: science and the sustainable intensifi cation of global agriculture RS Policy document 11/09 Issued: October 2009 RS1608 ISBN: 978-0-85403-784-1 © The Royal Society, 2009 Requests to reproduce all or part of this document should be submitted to: The Royal Society Science Policy 6–9 Carlton House Terrace London SW1Y 5AG Tel +44 (0)20 7451 2500 Email [email protected] Web royalsociety.org Design by Franziska Hinz, Royal Society, London Copyedited and Typeset by Techset Composition Limited Reaping the benefi ts: science and the sustainable intensifi cation of global agriculture Contents Foreword v Membership of working group vii Summary ix 1 Introduction 1 1.1 An urgent challenge 1 1.2 Trends in food crop production 2 1.3 Science in context 5 1.4 The need for sustainable intensifi cation 6 1.5 Agricultural sustainability 7 1.6 Agriculture and sustainable economic development 7 1.7 Other major studies 8 1.8 Further UK work 9 1.9 About this report 9 1.10 Conduct of the study 10 2 Constraints on future food crop production 11 2.1 Climate change 11 2.2 Water 11 2.3 Temperature 12 2.4 Ozone 13 2.5 Soil factors 13 2.6 Crop nutrition 15 2.7 Pests, diseases and weed competition 16 2.8 Energy and greenhouse -
Morphological Study of a Sugarcane Pest, Tryporyza (Scirpophaga) Nivella Fab (Lepidoptera: Pyralidae)
Indian Journal of Fundamental and Applied Life Sciences ISSN: 2231-6345 (Online) An Online International Journal Available at http://www.cibtech.org/jls.htm 2013 Vol. 3 (1) January-March, pp.6-8/Kumar and Rana Research Article MORPHOLOGICAL STUDY OF A SUGARCANE PEST, TRYPORYZA (SCIRPOPHAGA) NIVELLA FAB (LEPIDOPTERA: PYRALIDAE) *Praveen Kumar and K.S. Rana Department of Zoology, Agra College, Agra (U.P.) India *Author for Correspondence ABSTRACT Sugarcane (Saccharum officinarum L.) is a major cash crop of India. Production of Sugarcane is heavily decreased by the attack of insect pests in India. T. nivella is one of the serious pest of sugarcane which reduces the cane production in great quantity as well as in quality. For the morphological study of selected pest in laboratory, adults were collected from all selected sugarcane fields during the experimental year from April, 2011 to March, 2012 and then, they were released in net house of selected field in village-Nagla Kalua of District Etah (UP) in natural conditions. All morphological observations were recorded closely in natural condition with the help of Hand lens (Steriomicroscope and camera Lucida in laboratory). Males were silvery white in colour, 2.54 to 3.14 mm in length while females were crimson white in colour, 2.24 mm to 3.09 in length. Antennae were filiform type, 11 segmented and 3.0 mm mm long. Eggs were oval in shape, white in colour, 1.2 mm long and 1.0 wide in size. The prothorax was evident but compressed and reduced. The mesothorax was the largest and most prominent segment of the thorax. -
Study on the Screening of Sugarcane Varieties Against the Different Broods of T. Nivella
© 2020 JETIR October 2020, Volume 7, Issue 10 www.jetir.org (ISSN-2349-5162) STUDY ON THE SCREENING OF SUGARCANE VARIETIES AGAINST THE DIFFERENT BROODS OF T. NIVELLA Dr. Anita Kumari, Assistant Professor Dept. of Zoology, L. S. College Muzaffarpur Abstract Sugarcane is an agro - industrial crop and cultivation spread over a wide range of agro – ecological zone of India. The mature stalk is typically composed of 11 – 16 % fiber, 12 – 16 % soluble sugars, 2 – 3 % non – sugars and 63 – 73 % water. A sugarcane crop is sensitive to the climate, soil type, irrigation, fertilizers, insects, diseases control, varieties and the harvest period. The average yield of cane stalk is 60 – 70 tons per hectare per year. It varies between 20 and 180 tons per hectare per year depending on crop management approach. The long duration crop is attracted by T. nivella F., a notorious pest, which causes dead – heart disease to the plant. 24 varieties of sugarcane were screened and reveal that third and fourth brood showed minimum infestation while CoP 7104 and CoP 9103 were tolerant to the third and forth brood of the top borer. In September all the varieties registered maximum infestation. Yield loss due to infestation by different broods also confirms the above finding in different varieties. Keywords: Agro – industrial, infestation, T. nivella, CoP 7104 and CoP 9103 Sugarcane is the world’s largest crop by production quantity. In 2012, FAQ estimates it was cultivated on about 160 million hectares in more than 90 countries, with a worldwide harvest of 18.3 billion tons. Brazil was the largest producer of sugarcane in the world. -
Amurian Zoological Journal
ISSN 1999-4079 Амурский зоологический журнал Amurian zoological journal Том IX. № 4 Декабрь 2017 Vol. IX. No 4 December 2017 Амурский зоологический журнал ISSN 1999-4079 Рег. свидетельство ПИ № ФС77-31529 Amurian zoological journal Том IX. № 4. www.bgpu.ru/azj/ Vol. IX. № 4. Декабрь 2017 December 2017 РЕДАКЦИОННАЯ КОЛЛЕГИЯ EDITORIAL BOARD Главный редактор Editor-in-chief Член-корреспондент РАН, д.б.н. Б.А. Воронов Corresponding Member of R A S, Dr. Sc. Boris A. Voronov к.б.н. А.А. Барбарич (отв. секретарь) Dr. Alexandr A. Barbarich (exec. secretary) к.б.н. Ю. Н. Глущенко Dr. Yuri N. Glushchenko д.б.н. В. В. Дубатолов Dr. Sc. Vladimir V. Dubatolov д.н. Ю. Кодзима Dr. Sc. Junichi Kojima к.б.н. О. Э. Костерин Dr. Oleg E. Kosterin д.б.н. А. А. Легалов Dr. Sc. Andrei A. Legalov д.б.н. А. С. Лелей Dr. Sc. Arkadiy S. Lelej к.б.н. Е. И. Маликова Dr. Elena I. Malikova д.б.н. В. А. Нестеренко Dr. Sc. Vladimir A. Nesterenko д.б.н. М. Г. Пономаренко Dr. Sc. Margarita G. Ponomarenko к.б.н. Л.А. Прозорова Dr. Larisa A. Prozorova д.б.н. Н. А. Рябинин Dr. Sc. Nikolai A. Rjabinin д.б.н. М. Г. Сергеев Dr. Sc. Michael G. Sergeev д.б.н. С. Ю. Синев Dr. Sc. Sergei Yu. Sinev д.б.н. В.В. Тахтеев Dr. Sc. Vadim V. Takhteev д.б.н. И.В. Фефелов Dr. Sc. Igor V. Fefelov д.б.н. А.В. Чернышев Dr. Sc. Alexei V. Chernyshev к.б.н. -
Relative Abundance of Stem Borer Species and Natural Enemies in Rice Ecosystem at Madhupur,Tangail, Bangladesh
J. Bangladesh Agril. Univ. 12(2): 267–272, 2014 ISSN 1810-3030 Relative abundance of stem borer species and natural enemies in rice ecosystem at Madhupur, Tangail, Bangladesh M. M. Rahaman*, K.S. Islam, M. Jahan and M. A. A. Mamun Department of Entomology, Bangladesh Agricultural University, Mymensigh-2202, Bangladesh, *E-mail address: [email protected] Abstract The relative abundance of different stem borer species and their natural enemies with interaction effects were studied at three growth stages of irrigated Boro rice at Madhupur under the district of Tangail, Bangladesh during January to April, 2013. Five stem borer species viz; Yellow stem borer (Scirpophaga incertulas), Pink stem borer (Sesamia inferens), Dark headed stem borer (Chilo polyhcrysus), Stripped stem borer (Chilo suppressalis), White stem borer (Scirpophaga innotata), and nine different natural enemies were collected from the rice fields and recorded. The population of stem borers and natural enemies was highest in tillering stage and lowest in seedling stage. The relative abundance of stem borer species under investigation showed ranking order; yellow stem borer >dark headed stem borer>pink borer>white borer>stripped stem borer and natural enemies as ladybird beetle >long jawed spider>wolf spider>damselfly>carabid beetle>green mirid bug>lynx spider>dragon fly>ear wig. Populations of all five stem borers were positively correlated with ladybird beetle, wolf spider, long jawed spider, lynx spider, damsel fly, dragon fly, green mirid bug and negatively correlated with carabid beetle and earwig. Keywords: Stem borers, Natural enemies, Relative abundance, Rice ecosystem Introduction Rice (Oryza sativa L.) is one of the world’s most important crops providing a staple food for nearly half of the global population (FAO, 2004). -
Biocontrol of Insect Pests of Sugarcane (Saccharum Sp
BIOCONTROL OF INSECT PESTS OF SUGARCANE ( SACCHARUM SP .) By *Zia-ul-Hussnain, Asia Naheed and Saadia Rizwana *Shakarganj Sugar Research Institute, Jhang, PAKISTAN ABSTRACT Sugarcane ( Saccharum sp.) crop is habitat of more than 1500 species of insects in the sugar world. Amongst these borers like Scirpophaga excerptalis , Chilo infuscatellus , Emmalocera depresella , Acigona steniellus and sucking insect pest Pyrilla perpusilla are major devastators to cause considerable loss in yield and quality of the crop. In our country primary and secondary insect pests species identified were 11, while parasites and predators species were 14 in cane fields. Studies revealed that with 75 % infestation of borers, sucrose recovery was 52 % lower. Field data on infestation of Pyrilla perpusilla showed that decline in cane yield was 18%. Biocontrol of insect pest of sugarcane crop through artificial rearing of Trichogramma chilonis was initiated from 1999 and for Chrysoperla carnea from 2002 at the institute . Results of the field studies revealed that under unreleased area of Trichogramma chilonis, the infestation of borers was 11.65% and in the released area it was 2.74 %. The acreage covered with the released of the parasites was more than 50 thousands from last five years in cane growing areas of Shakarganj Mills. It was observed that under controlled conditions Chrysoperla carnea larvae predated 80% eggs of Pyrilla perpusilla . Efficiency of biocontrol of the insect pest can be improved only with integrated management practices of the crop identified, these are resistant varieties, alternate planting dates, trash blanketing of ratoon crop, early harvesting, balanced fertigation, pest-free seed cane and field monitoring. -
~ New Record of Granulovirus on Sugarcane Top Borer, Sclrpophaga Excerptalis Walker (Lepidoptera: Pyralidae)
1. BioI. Control, 13: 133-135, 1999 Research Note ~ new record of granulovirus on sugarcane top borer, SClrpophaga excerptalis Walker (Lepidoptera: Pyralidae) B. SINGARAVELU, R. NIRMALA and S. EASWARAMOORTHY Section of Entomology, Sugarcane Breeding Institute Coimbatore 641 007, Tamil N adu, India A~STRACT: A gran~lovirus was found to infect sugarcane top borer, SClrp?pha,?a exc~rpt~lzs Walker for the first time. In a preliminary study, the v~us Infechon In field population was found to vary from 1.6 to 14.4 (X=.S.8) per cent. In the laboratory test, the virus caused mortality of final Instar larvae up to 55.2 per cent in 4 to 8 days after infection. KEY WORDS: Baculovirus, granulovirus, natural infection, pathogenicity, sugarcane top borer The top borer, Scirpophaga excerptalis the larvae were verticalIy planted on moist Walker (Lepidoptera: Pyralidae) is unique sand bed. The bed was kept moist to among the moth borers of sugarcane in that prevent shrinkage of the cane bits unti I the it is prevalent in all the sugarcane regions study was completed. The larvae in the cane and attacks all stages of the crop bits were examined periodically for (Mukunthan, 1986). In a field survey made mortality or pupation. Tissue smears of around Coimbatore for natural enemies, dead larvae and pupae were examined for some of the larvae were found dead. the presence of virus particles and the level Examination of haemolymph and tissue of virus infection was determined. The smears of dead larvae, under dark field infection level varied from 1.6 per cent microscope, revealed the presence of a during March, 1999 to 14.4 per cent in granulovirus, which was further confirmed December, 1998 with a mean of 5.8 per by electron microscopic examination.