Hematopoietic Stem Cell Marker Antibody Panel (ABCG2, CD34, Store At: Prominin1)

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ARG30135 Package: 1 pair Hematopoietic Stem Cell Marker Antibody Panel (ABCG2, CD34, Store at: Prominin1)

Component

Cat No Component Name Host clonality Reactivity Application Package

ARG62820 anti-CD34 antibody Mouse mAb Hu Blocking, FACS, 50 μg [4H11(APG)] ICC/IF

ARG54138 anti-Prominin-1 Mouse mAb Hu WB 50 μl antibody [6H10-F1-C11]

ARG51346 anti-ABCG2 / CD338 Rabbit pAb Hu WB 50 μl antibody

ARG65350 Goat anti-Mouse IgG Goat pAb ELISA, IHC, WB 50 μl antibody (HRP)

ARG65351 Goat anti-Rabbit IgG Goat pAb Rb ELISA, IHC, WB 50 μl antibody (HRP)

Summary

Product Description Hematopoietic Stem Cells (HSCs) are the mesodermal progenitor cells that give rise to all types of blood cells including monocytes, macrophages, neutrophils, basophils, eosinophils, erythrocytes and other cells related to lymphoid lineage. It has been a challenge for researchers using HSCs because of the difficulties to isolate them from a large pool of cells because the number of HSCs in each organism is scarce and they appear very similar to lymphocytes morphologically. CD34, CD133 and ABCG2 are reliable marker for HSCs. Using the antibodies included in arigo’s Hematopoietic Stem Cell Panel, researchers can easily identify HSCs from the blood cell population. Sutherland, D.R. et al. (1992) J. Hematother.1:115. Yin, A.H. et al. (1997) Blood 90:5002. Gehling, U.M. et al. (2000) Blood 95:3106. Zhou, S. et al. (2001) Nat. Med. 7:1028. Kim, M. et al. (2002) Clin. Cancer Res. 8:22.

www.arigobio.com 1/2 Images

ARG51346 anti-ABCG2 / CD338 antibody WB validated image

Western Blot: extract from HL-60 cells stained with anti-ABCG2 / CD338 antibody ARG51346

ARG54138 anti-Prominin-1 antibody [6H10-F1-C11] WB validated image

Western Blot: Prominin-1 in CaCo2 cell lysate stained with Prominin-1 antibody [6H10-F1-C11] (ARG54138) (diluted in 1: 1000). Predicted band size: 97KDa. Observed band size: 133KDa

ARG54138 anti-Prominin-1 antibody [6H10-F1-C11] WB validated image

Western Blot: Prominin-1 expression in CHO-k1 cells non- transfected (A) or transfected (B) with Prominin-1 and stained with Prominin-1 antibody [6H10-F1-C11] (ARG54138) (diluted in 1: 1000). Predicted band size: 97KDa. Observed band size: 133KDa.