R&D Systems Tools for Cell Biology Research™
STEM CELL FOCUS: CANCER STEM CELLS Cancer Stem Cell Markers
FEATURED DATA: Aminopeptidase N · BMI-1 · CD24 · CD44 · E-Cadherin · EpCam/TROP1 · ErbB2/Her2 · STRO‑1 · Tumorspheres
The CSC Hypothesis Like all stem cells, oncogenically transformed cancer stem cells (CSCs) are defined by their ability for self-renewal and multipotency. The CSC hypothesis states that, although CSCs represent a rare population of cells within a tumor, their high tumorigenic capacity drives tumorigenesis. Due to their intrinsic stem cell-like properties, CSC proliferation gen erates more CSCs and all the differentiated cell types that compose the bulk of the tumor. Differentiated cancer cells have been shown to proliferate at a faster rate than CSCs but have little tumor-initiating potential. Because CSCs exhibit increased resistance to toxic and chemical insults, this specific subpopulation of cells is believed to underlie resistance to chemotherapy and disease relapse. In fact, the CSC model suggests that all CSCs must be eradicated to eliminate a tumor and prevent its recurrence. Malignancy-specific Markers Available for: Unlike the differentiated cancer cells in a Bladder Cancer Lung Cancer tumor, CSCs are capable of forming new tumors when xenotransplanted into Breast Cancer Melanoma immunodeficient animal models. Although Colon Cancer Myeloma inconsistencies in these studies have questioned the validity of the CSC hypothesis, Gastric Cancer Osteosarcoma it is likely that variable findings result from differences in assay sensitivity and Glioma/Medulloblastoma Ovarian Cancer quantification methodology. An overriding Head & Neck Cancer Pancreatic Cancer experimental limitation for CSC researchers is the ability to identify and isolate CSCs from Leukemia Prostate Cancer other tumor cells. To address this need, we Liver Cancer present a table of molecules identified in the current literature as markers of CSCs detected Tissue Specific Expression of Cancer Stem Cell (CSC) Markers. This illustration depicts a tumor containing a small population of CSCs. For more information and in specific malignancies. a list of products for malignancy-specific CSC markers, please visitwww.RnDSystems.com/CSC .
For more information, please visit: www.RnDSystems.com/CSC R&D Systems Products for Cancer Stem Cell Markers ELISAs ELISAs RECOMBINANT CELL SELECTION RECOMBINANT CELL SELECTION MOLECULE & NATURAL ANTIBODIES & DETECTION MOLECULE & NATURAL ANTIBODIES & DETECTION PROTEINS KITS & REAGENTS PROTEINS KITS & REAGENTS Bladder Cancer Stem Cell Markers Musashi-1 H (IHC, WB)
CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H Gastric Cancer Stem Cell Markers R (B/N, FC, IHC, WB) Ca (FC) CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H CD47 H H (B/N, FC, IHC, WB) M (FC, IHC, WB) R (B/N, FC, IHC, WB) Ca (FC)
Breast Cancer Stem Cell Markers DLL4 H M H (FC, IHC, WB) M (ELISA, WB) M
Aldehyde H H (IHC, IP, WB) Glioma/Medulloblastoma Cancer Stem Cell Markers Dehydrogenase 1-A1/ ALDH1A1 A20/TNFAIP3 H
BMI-1 H (ChIP, FC, IHC, WB) H ABCG2 H (FC, IHC)
CD24 H H (FC, IHC, WB) H CD49f/Integrin α6 H (B/N, FC, IHC, IP, WB) M (B/N, FC, IHC) B (B/N, FC, IHC) CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H R (B/N, FC, IHC, WB) Ca (FC) CD126/IL-6 Rα H M H (B/N, ELISA, FC, IHC, WB) H M M (B/N, ELISA, FC, IHC, WB) CD49f/Integrin α6 H (B/N, FC, IHC, IP, WB) M (B/N, FC, IHC) B (B/N, FC, IHC) CD171/NCAM-L1 H M H (IHC, WB) M (FC)
CD126/IL-6 Rα H M H (B/N, ELISA, FC, IHC, WB) H M CX3CR1 H (FC, WB) M (FC, WB) M (B/N, ELISA, FC, IHC, WB) CXCR4 H (B/N, FC, IHC) M (B/N, FC, IHC) F (FC, IHC) H CXCR1/IL-8 RA H (B/N, FC, IHC) CX3CL1/Fractalkine H M R H (B/N, ELISA, FC, IHC, WB) M (B/N, ELISA, WB) H M R CXCR4 H (B/N, FC, IHC) M (B/N, FC, IHC) F (FC, IHC) H R (B/N, ELISA, IHC, WB)
DLL4 H M H (FC, IHC, WB) M (ELISA, WB) M HIF-2α/EPAS1 H (ChIP, IHC, WB) M (IHC, WB) R (ChIP, IHC, WB) H
EpCAM/TROP1 H H (ELISA, FC, IHC, WB) H H Musashi-1 H (IHC, WB)
ErbB2/Her2 H H (B/N, ELISA, FC, IHC, WB) M (FC, IHC, WB) H H c-Myc H (ChIP, IHC, WB) H
GLI-1 H (ChIP, IHC, WB) M (IHC, WB) H Nestin H (FC, IHC) M (FC, IHC, WB) R (FC, IHC, WB)
GLI-2 H (ChIP, IHC, WB) M (IHC, WB) H Podoplanin H M H (FC, IHC, WB) M (IHC, WB)
PTEN H H (FC, IHC, WB) M (FC, IHC, WB) R (FC, IHC, WB) H M R Head & Neck Cancer Stem Cell Markers Colon Cancer Stem Cell Markers ABCG2 H (FC, IHC)
Aldehyde H H (IHC, IP, WB) Aldehyde H H (IHC, IP, WB) Dehydrogenase 1-A1/ Dehydrogenase 1-A1/ ALDH1A1 ALDH1A1
CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H BMI-1 H (ChIP, FC, IHC, WB) H R (B/N, FC, IHC, WB) Ca (FC) CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H CD26/DPPIV H M H (ELISA, FC, IHC, WB) M (ELISA, FC, IHC, IP, WB) H M R (B/N, FC, IHC, WB) Ca (FC)
CD166/ALCAM H M H (B/N, ELISA, FC, WB) M (FC, IHC, WB) H M Leukemia Cancer Stem Cell Markers
EpCAM/TROP1 H H (ELISA, FC, IHC, WB) H H BMI-1 H (ChIP, FC, IHC, WB) H
GLI-1 H (ChIP, IHC, WB) M (IHC, WB) H CD34 H (FC, IHC) M (FC, WB) R (FC, IHC, WB) Ca (FC, WB) P (WB) Species Key: H Human M Mouse R Rat B Bovine Ca Canine CR Cotton Rat F Feline P Porcine Application Key: B/N Blocking/Neutralization ChIP Chromatin Immunoprecipitation ELISA ELISA Capture and/or Detection FC Flow Cytometry IHC Immunohistochemistry IP Immunoprecipitation WB Western blot
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100
80
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40 Relative Cell Number Cell Relative 20
0 100 101 102 103 104 BMI-1
EpCAM/TROP-1 in the HT-29 Human Cell Line. Epithelial Cellular Adhesion Intracellular Detection of BMI-1 by Flow Cytometry. The HeLa human cervical Aminopeptidase N in Human Liver Cancer Tissue. Aminopeptidase N/ANPEP Molecule (EpCAM/TROP1) was detected in immersion-fixed HT-29 human colon epithelial carcinoma cell line was stained with an APC-conjugated Mouse Anti- was detected in immersion-fixed paraffin-embedded sections of human liver adenocarcinoma cells using a Mouse Anti-Human EpCAM/TROP1 Monoclonal Human BMI-1 Monoclonal Antibody (Catalog # IC33341A; filled histogram) or an cancer tissue using a Sheep Anti-Human Aminopeptidase N Antigen Affinity-
Antibody (Catalog # MAB960). The cells were stained using the NorthernLights™ APC-conjugated Mouse IgG2A Isotype Control Antibody (Catalog # IC003A; open purified Polyclonal Antibody (Catalog # AF3815). The tissue was stained using the 493-conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # NL009; histogram). Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS019; brown) and green) and the nuclei were counterstained with DAPI (blue). Specific staining was counterstained with hematoxylin (blue). Specific labeling was localized to bile localized to the cell surface and the cytoplasm. canaliculi.
www.RnDSystems.com/CSC For more information, please visit: www.RnDSystems.com/CSC
ELISAs ELISAs RECOMBINANT CELL SELECTION RECOMBINANT CELL SELECTION MOLECULE & NATURAL ANTIBODIES & DETECTION MOLECULE & NATURAL ANTIBODIES & DETECTION PROTEINS KITS & REAGENTS PROTEINS KITS & REAGENTS CD38 H M H (FC, IHC, IP, WB) M (FC, WB) CD271/NGF R/ H M H (FC, IHC, WB) M (IHC, WB) M TNFRSF16 CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H R (B/N, FC, IHC, WB) Ca (FC) Nestin H (FC, IHC) M (FC, IHC, WB) R (FC, IHC, WB)
CD47 H H (B/N, FC, IHC, WB) M (FC, IHC, WB) Myeloma Cancer Stem Cell Markers
CD96 M H (FC, WB) M (FC, WB) CD19 H (FC)
CD117/SCF R/c-kit H H (B/N, ELISA, FC, IHC, WB) M (FC, IHC, WB) H CD20/MS4A1 H (FC)
CD123/IL-3 Rα H M H (B/N, FC, IHC, WB) M (FC, WB) CD27/TNFRSF7 H M H (B/N, FC, IHC, WB) M (ELISA, FC, WB) M
GLI-1 H (ChIP, IHC, WB) M (IHC, WB) H CD38 H M H (FC, IHC, IP, WB) M (FC, WB)
GLI-2 H (ChIP, IHC, WB) M (IHC, WB) H CD138/Syndecan-1 H M H (ELISA, FC, IHC, WB) M (FC, IHC, IP, WB) H
MICL/CLEC12A H (FC, IHC, WB) M (FC, IHC, WB) Osteosarcoma Cancer Stem Cell Markers
Musashi-2 H (IHC, WB) ABCG2 H (FC, IHC)
TIM-3 H M H (FC, WB) M (FC, WB) H Nestin H (FC, IHC) M (FC, IHC, WB) R (FC, IHC, WB)
Liver Cancer Stem Cell Markers STRO-1 H (FC, IHC)
α-Fetoprotein/AFP H (FC, IHC, WB) M (FC, IHC, WB) H Ovarian Cancer Stem Cell Markers
Aminopeptidase N/ H M H (FC, IHC, IP, WB) M (FC, IHC, IP, WB) CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H ANPEP R (B/N, FC, IHC, WB) Ca (FC)
CD90/Thy1 H (FC, WB) M (WB) CD117/SCF R/c-kit H H (B/N, ELISA, FC, IHC, WB) M (FC, IHC, WB) H
NF2/Merlin H (IHC, WB) Pancreatic Cancer Stem Cell Markers
Lung Cancer Stem Cell Markers BMI-1 H (ChIP, FC, IHC, WB) H
ABCG2 H (FC, IHC) CD24 H H (FC, IHC, WB) H
Aldehyde H H (IHC, IP, WB) CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H Dehydrogenase 1-A1/ R (B/N, FC, IHC, WB) Ca (FC) ALDH1A1 CXCR4 H (B/N, FC, IHC) M (B/N, FC, IHC) F (FC, IHC) H CD90/Thy1 H (FC, WB) M (WB) EpCAM/TROP1 H H (ELISA, FC, IHC, WB) H H CD117/SCF R/c-kit H H (B/N, ELISA, FC, IHC, WB) M (FC, IHC, WB) H Prostate Cancer Stem Cell Markers EpCAM/TROP1 H H (ELISA, FC, IHC, WB) H H ABCG2 H (FC, IHC) Melanoma Cancer Stem Cell Markers BMI-1 H (ChIP, FC, IHC, WB) H ABCB5 H (WB) CD44 H M R P H (FC, IHC, IP, WB) M (B/N, FC, IHC, WB) H ABCG2 H (FC, IHC) R (B/N, FC, IHC, WB) Ca (FC)
CD20/MS4A1 H (FC) c-Myc H (ChIP, IHC, WB) H
CD166/ALCAM H M H (B/N, ELISA, FC, WB) M (FC, IHC, WB) H M Integrin a2b1 H
Species Key: H Human M Mouse R Rat B Bovine Ca Canine CR Cotton Rat F Feline P Porcine Application Key: B/N Blocking/Neutralization ChIP Chromatin Immunoprecipitation ELISA ELISA Capture and/or Detection FC Flow Cytometry IHC Immunohistochemistry IP Immunoprecipitation WB Western blot
STRO‑1 in the Human MG‑63 Cell Line. Stromal Cell Precursor Surface Antigen ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in Tumorsphere Formation using the StemXVivo™ Serum-Free Tumorsphere (STRO‑1) was detected in immersion-fixed MG‑63 human osteosarcoma cells using immersion-fixed paraffin-embedded sections of human breast cancer tissue using Media. The MCF-7 human breast cancer cell line was cultured for 7 days in a Mouse Anti-Human STRO‑1 Monoclonal Antibody (Catalog # MAB1038). The cells a Mouse Anti-Human ErbB2 Monoclonal Antibody (Catalog # MAB11291). The StemXVivo Serum-Free Tumorsphere Media (Catalog # CCM012) to induce were stained using the NorthernLights 557-conjugated Donkey Anti-Mouse IgG tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit tumorsphere formation. The morphology of MCF-7 tumorspheres was documented Secondary Antibody (Catalog # NL007; red) and the nuclei were counterstained (Catalog # CTS002; brown) and counterstained with hematoxylin (blue). Specific using a CloneSelect™ Imager from Molecular Devices (New Milton) Limited. with DAPI (blue). Specific staining was localized to the cell surface and the labeling was localized to the cytoplasm of cancer cells. cytoplasm.
For research use only. Not for use in diagnostic procedures. MagCellect™ Cell Selection Kits for Cancer Stem Cell Research MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit The MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit (Catalog # MAGH111) was designed to isolate a small population of human breast cancer cells that have the unique ability to form new tumors in mice.1 This kit utilizes a two-step procedure that combines negative and positive selection techniques. CD24low/– cells are initially enriched by negative selection whereby unwanted CD24+ cells are tagged and magnetically removed. CD44+ cells are subsequently isolated from the CD24low/– population by positive selection using a biotinylated human CD44 antibody, streptavidin- conjugated magnetic beads, and a MagCellect magnet (Catalog # MAG997, or equivalent). The efficiency of enrichment can be assessed by staining the recovered cells with fluorochrome-conjugated anti-human CD24 and CD44 detection antibodies provided in the kit. Reference 1. Al-Hajj, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3983.
Features MagCellect Kit No cell damage induced by beads or ferrofluids CATALOG # KIT CONTENTS MagCellect CD24–CD44+ Breast Cancer Stem Cell MAGH111 Biotinylated CD24 Selection Antibody, Biotinylated CD44 Selection Antibody, Fast – target cells can be enriched in approximately 1 hour Isolation Kit APC-conjugated CD24 Detection Antibody, PE-conjugated CD44 Detection Antibody, Streptavidin Ferrofluid, 10X MagCellect Buffer Flexible – compatible with several magnet systems
Assay Principle ENRICHMENT OF CD24low/ CELLS ENRICHMENT OF CD44+ CELLS ASSESSMENT OF ENRICHMENT EFFICIENCY Biotinylated CD24 Biotinylated CD44 APC-conjugated CD24 PE-conjugated Antibody Antibody Detection Antibody Streptavidin-conjugated Streptavidin-conjugated CD44 Detection magnetic beads magnetic beads Antibody Biotin CD24low/ Cells CD24low/ CD44+ Cells
Analysis of CD24low/–CD44+ Cells using the MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit. Undesired CD24+ cells are bound by a biotinylated anti-human CD24 antibody and captured by streptavidin-conjugated magnetic particles. These cells are then isolated using a MagCellect Magnet (Catalog # MAG997, or equivalent), and an enriched CD24low/– population is aspirated from the sample solution. CD44+ cells are subsequently labeled with a biotinylated anti-human CD44 antibody and magnetically tagged with streptavidin-conjugated magnetic particles. CD24low/–CD44+ cells are captured by applying the MagCellect Magnet, or any compatible system, and undesired cells are aspirated from solution.
A. BEFORE ENRICHMENT B. AFTER ENRICHMENT C. ENRICHMENT PROFILE 104 104 104 104 104 104 50 50 50
103 103 103 103 103 103 40 40 40
30 30 30 102 102 102 102 102 102 CD44 CD44 CD44 CD44 CD44 CD44 20 20 20BEFORE BEFORE BEFORE 101 101 101 101 101 101 Relative Cell Number Cell Relative 10 Number Cell Relative 10 Number Cell Relative 10 AFTER AFTER AFTER 100 100 100 100 100 100 0 0 0 1 2 1 3 2 1 4 3 2 4 3 4 1 2 1 3 2 1 4 3 2 4 3 4 100 10 100 10 10100 10 1010 10 1010 1010100 1010 100 10 10100 10 1010 10 1010 1010100 10 101100 1021001 10103 12 104101023 101043 104 CD24 CD24 CD24 CD24 CD24 CD24 CD24 CD24 CD24
Analysis of CD24low/–CD44+ Cells Isolated using the MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit. A population of CD24low/–CD44+ cells was isolated from the MCF-7 human breast cancer cell line using the MagCellect CD24–CD44+ Breast Cancer Stem Cell Isolation Kit (Catalog # MAGH111). CD24low/–CD44+ cells (upper left quadrant), before (A) and after (B) enrichment, were detected by double-staining with APC-conjugated Mouse Anti-Human CD24 and PE-conjugated Mouse Anti-Human CD44 Detection Antibodies (both provided in the kit). A histogram profiling the enrichment of CD24low/– cells (filled histogram) from the original cell population (open histogram) is also shown C( ).
For more information, please visit: www.RnDSystems.com/MagCellect
www.RnDSystems.com/CSC PlusCellect™ Cell Selection Kits for Cancer Research PlusCellect Cell Selection Kits offer a simple and efficient method to enrich specific populations of cells. These kits are the only cell isolation system on the market that includes two independent antibodies, a biotinylated selection antibody for the purification of target cells via positive selection, and a fluorochrome-conjugated detection antibody for the assessment of enrichment efficiency.
Features PlusCellect Kits for Cancer Research Positive selection for the isolation of rare cell populations AVAILABLE KITS CATALOG # KIT CONTENTS Human ACE/CD143+ Cancer/Endothelial Cells Selection & detection antibodies recognizing different epitopes are provided PlusCellect Kit PLS929 + May be used in conjunction with negative selection for even greater purity Human CD44 Cancer Cells PlusCellect Kit PLS4948 Human CXCR4+ Cancer/Stem Cells PlusCellect Kit PLS170 Flexible – compatible with several magnet systems Human E-Cadherin+ Cancer/Stem Cells PlusCellect Kit PLS1838 Biotinylated Analyte-specific Fast – target cells can be enriched in approximately 1 hour + Human EGF R/ErbB1 Cancer Cells PlusCellect Kit PLS1095 Selection Antibody, Human EpCAM+ Cancer Cells PlusCellect Kit PLS960 PE-conjugated Analyte- Human ErbB2/HER2+ Cancer Cells PlusCellect Kit PLS1129 specific Detection Antibody, Assay Principle PlusCellect Buffer Human HGF R/c-Met+ Cancer Cells PlusCellect Kit PLS358 Fluorochrome-conjugated Human MCAM/CD146+ Cancer/Endothelial Cells Biotinylated PlusCellect Kit PLS932 Analyte-speci c Analyte-speci c Antibody Detection Antibody Human Nectin-4+ Cancer Cells PlusCellect Kit PLS2659 Streptavidin-conjugated magnetic beads Mouse E-Cadherin+ Cancer/Stem Cells PlusCellect Kit PLS748 Undesired Biotin Cells
Desired For more information, please visit: Cells www.RnDSystems.com/PlusCellect
Isolation of Cell Populations using PlusCellect Kits for Positive Selection. Cells of interest are tagged with a biotinylated antibody and subsequently bound to streptavidin-conjugated magnetic particles (MagCellect Streptavidin Ferrofluid, Catalog # MAG999, or equivalent). Tagged cells are then isolated using a compatible magnet system (MagCellect Magnet, Catalog # MAG997, or equivalent), while untagged cells are removed by aspirating the media. The desired cells are subsequently resuspended in PlusCellect Buffer, and the efficiency of enrichment is assessed with the PE-conjugated detection antibody provided in the kit.
A. BEFORE ENRICHMENT B. AFTER ENRICHMENT C. ENRICHMENT PROFILE
104 104104 104 104104 80 8080
3 3 BEFOREBEFOREBEFORE AFTERAFTERAFTER 103 1010 2.5% 103 103103 92% 60 6060
102 102102 2 102102 10 40 4040 E-Cadherin E-Cadherin E-Cadherin E-Cadherin E-Cadherin E-Cadherin Relative Cell Number Cell Relative Number Cell Relative 101 101101 101 101101 Number Cell Relative 20 2020
97% 7% 0 0 0 100 100100 0 100100 10 0 0 0 1 1 1 2 2 2 3 3 3 4 4 4 100 100100 101 101101 102 102102 103 103103 104 104104 100 100100 101 101101 102 102102 103 103103 104 104104 10 1010 10 1010 10 1010 10 1010 10 1010 EpCamEpCamEpCam EpCamEpCamEpCam EpCamEpCamEpCam
Isolation of Human EpCAM+ Cells using the Human EpCAM+ Cancer Cells PlusCellect Kit. Human EpCAM+ cells were isolated from a mixture of whole blood cells and the EpCAM-expressing MCF-7 human breast cancer cell line using the Human EpCAM+ Cancer Cells PlusCellect Kit (Catalog # PLS960). Cells were stained with an APC-conjugated Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # FAB18381A) and a PE-conjugated Goat Anti-Human EpCAM Antibody (provided in the kit) before (A) and after (B) selection. A histogram of the enrichment profile is also shown C( ).
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R&D Systems is a registered trademark of TECHNE Corporation. MagCellect, PlusCellect, NorthernLights, and StemXVivo are trademarks of R&D Systems. CloneSelect is a trademark of Molecular Devices. FC_01.12_CSCmarkers Peer-reviewed References for R&D Systems Cancer Stem Cell-related Products
1. McLean, K. et al. (2011) Human ovarian carcinoma- 3. Dittmar, T. et al. (2011) Characterization of hybrid cells 6. Buijs, J. et al. (2011) The BMP2/7 heterodimer inhibits the associated mesenchymal stem cells regulate cancer stem derived from spontaneous fusion events between breast human breast cancer stem cell subpopulation and bone cells and tumorigenesis via altered BMP production. epithelial cells exhibiting stem-like characteristics and metastases formation. Oncogene Epub ahead of print. J. Clin. Invest. 121:3206. breast cancer cells. Clin. Exp. Metastasis 28:75. PE-conjugated Mouse Anti-Human CD44s Pan PE-conjugated Mouse Anti-Human CD90/Thy1 Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Specific Monoclonal Antibody (Catalog # FAB4948P) Monoclonal Antibody (Catalog # FAB2067P) Antibody (Catalog # MAB330) APC-conjugated Mouse Anti-Human CD24 Sample: Human carcinoma-associated mesenchymal Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Monoclonal Antibody (Catalog # FAB5247A) stem cells Antibody (Catalog # MAB331) Application: Flow cytometry Sample: MDA-MB-231 human breast cancer cell line PE-conjugated Mouse Anti-Human CXCR4 Application: Flow cytometry Recombinant Human BMP-2 (Catalog # 355-BM) Monoclonal Antibody (Catalog # FAB173P) Recombinant Human BMP-2 (Catalog # 355-BM) Sample: Human mesenchymal stem cells PE-conjugated Mouse Anti-Human CCR7 Application: Stimulation Monoclonal Antibody (Catalog #FAB197P) Recombinant Human BMP-7 (Catalog # 354-BP) Recombinant Human BMP-2/BMP-7 Heterodimer Recombinant Human Noggin (Catalog # 3344-NG) PE-conjugated Goat F(ab’)2 Anti-Mouse IgG (H+L) Secondary Antibody (Catalog # F0102B) (Catalog # 3229-BM) Sample: Human ovarian tumor cells Application: Tumor sphere assay and in vivo (mouse) Sample: Human breast epithelial/breast cancer Sample: MDA-MB-231 human breast cancer cell line hybrid cells Application: Migration 2. Leis, O. et al. (2011) Sox2 expression in breast tumours and Application: Flow Cytometry activation in breast cancer stem cells. Oncogene Epub 7. Kondo, S. et al. (2011) Epstein-Barr virus latent membrane ahead of print. 4. Chang, C. et al. (2011) p53 regulates epithelial- protein 1 induces cancer stem/progenitor-like cells in mesenchymal transition and stem cell properties through nasopharyngeal epithelial cell lines. J. Virol. 85:11255. Proteome Profiler™ Human Pluripotent Stem Cell modulating miRNAs. Nat. Cell Biol. 13:317. Array Kit (Catalog # ARY010) PE-conjugated Mouse Anti-Human ALCAM/CD166 Mouse Anti-Human BMI-1 Monoclonal Antibody Monoclonal Antibody (Catalog # FAB6561P) Sample: MCF-7 human breast cancer cell line-derived (Catalog # MAB33342) mammosphere lysates PE-conjugated Mouse Anti-Human ABCG2 Application: Profiling of protein expression Sample: Human breast cancer tissue Monoclonal Antibody (Catalog # FAB995P) Application: Immunohistochemistry Goat Anti-Human Nanog Antigen-affinity Purified Sample: EBV-LMP1 stimulated human nasopharyngeal Polyclonal Antibody (Catalog # AF1997) 5. Jan, M. et al. (2011) Prospective separation of normal and epithelial cell lines leukemic stem cells based on differential expression of Application: Flow cytometry Sample: Human breast cancer tissue TIM3, a human acute myeloid leukemia stem cell marker. Application: Immunohistochemistry Proc. Natl. Acad. Sci. USA 108:5009. Methylcellulose Stock Solution (Catalog # HSC001) Rat Anti-Human TIM-3 Monoclonal Antibody Sample: MCF-7 human breast cancer cell line-derived (Catalog # MAB2365) mammospheres Sample: Human acute myeloid leukemia cells Visit us at ISSCR 2012 Application: Cell culture Application: Flow cytometry June 13–16th Yokohama, Japan