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Differentially Expressed Genes

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Average Fold ProbeID Primary Accession Number Gene Symbol Gene Name Change Pair 1 Pair 1 Pair 2 Pair 2 Up-regulated in MMLV-Cdc25a,MMLV-Neu tumors compared to MMLV-Neu tumors A_51_P309754 NM_019910 Dcpp demilune cell and parotid protein 19.1 4444 A_52_P497392 ENSMUST00000075966 NA NA 17.8 4444 A_51_P236486 NM_145386 BC005655 cDNA sequence BC005655 17.1 4444 A_51_P236483 NM_145386 BC005655 cDNA sequence BC005655 16.8 4444 A_52_P535962 BG866746 Dcpp demilune cell and parotid protein 14.5 4444 A_52_P455370 NM_175628 A2m alpha-2-macroglobulin 5.4 2223 A_51_P508899 NM_022020 Rbp7 retinol binding protein 7, cellular 4.7 2222 A_52_P602847 NM_008134 Glycam1 glycosylation dependent cell adhesion molecule 1 4.7 22NaNNaN A_52_P343627 NM_022020 Rbp7 retinol binding protein 7, cellular 4.4 2 2 2 NaN A_52_P669035 NM_009899 Clca1 chloride channel calcium activated 1 4.1 231 2 A_51_P265806 NM_030601 Clca2 chloride channel calcium activated 2 3.6 221 2 A_51_P382849 NM_010330 Emb embigin 3.5 221 2 A_51_P147034 NM_027407 Als2cr15 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 15 2.7 2 2 1 1 A_51_P311159 NM_012044 Pla2g2e phospholipase A2, group IIE 2.7 2 2 11 A_51_P295315 NM_020033 Ankrd2 ankyrin repeat domain 2 (stretch responsive muscle) 2.7 2 1 1 1 A_52_P1068810 AK076354 NA NA 2.7 2 2 1 1 A_51_P213725 XM_136658 4930431J08Rik RIKEN cDNA 4930431J08 gene 2.7 2 2 11 A_52_P30302 AK032696 5830433M19Rik RIKEN cDNA 5830433M19 gene 2.7 2 2 1 1 A_52_P280469 NAP048817-1 NA NA 2.7 2 1 1 1 A_52_P106560 NM_146403 Olfr1295 olfactory receptor 1295 2.7 2 2 1 1 A_52_P366105 BC027355 BC039161 cDNA sequence BC039161 2.7 2 111 A_51_P261494 AK053463 Birc7 baculoviral IAP repeat-containing 7 (livin) 2.7 2 1 11 A_51_P167292 NM_009892 Chi3l3 chitinase 3-like 3 2.6 2 2 1 1 A_52_P541118 NM_175692 A930034L06Rik RIKEN cDNA A930034L06 gene 2.6 2 2 1 1 A_52_P1003906 AK046946 NA NA 2.6 2 1 1 1 A_51_P171616 NM_009518 Wnt10a wingless related MMTV integration site 10a 2.6 2 111 A_51_P301007 NM_029299 Spata19 spermatogenesis associated 19 2.6 2 2 1 1 A_52_P44054 XM_132015 BC037112 cDNA sequence BC037112 2.6 2 2 11 A_52_P1100434 AK081327 NA NA 2.6 2 1 1 1 A_52_P351638 NM_020275 Tnfrsf10b tumor necrosis factor receptor superfamily, member 10b 2.6 2 1 1 1 A_52_P464346 AK036335 Atf6 activating transcription factor 6 2.6 2 1 1 1 A_52_P615401 NM_011906 Gpr175 G protein-coupled receptor 175 2.6 2 1 1 1 A_51_P390239 NM_032396 Kremen1 kringle containing transmembrane protein 1 2.6 2 1 1 1 A_52_P617386 AK084084 4632427E13Rik RIKEN cDNA 4632427E13 gene 2.6 2 1 11 A_52_P955185 AK031453 NA NA 2.6 2 1 1 1 A_51_P258620 NM_029391 Rab4b RAB4B, member RAS oncogene family 2.6 2 1 1 1 A_51_P410715 NM_018805 Hs3st3b1 heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 2.6 1 111 A_52_P150236 NM_018816 Apom apolipoprotein M 2.6 2 1 1 1 A_51_P183261 NM_007474 Aqp8 aquaporin 8 2.6 2 1 1 1 A_52_P309337 BE570772 NA NA 2.6 2 1 1 1 A_52_P35377 NM_018807 Plagl2 pleiomorphic adenoma gene-like 2 2.6 2 1 1 1 A_52_P478460 AK078259 5730457F11Rik RIKEN cDNA 5730457F11 gene 2.6 2 111 A_52_P686231 NM_177014 A530020G20Rik RIKEN cDNA A530020G20 gene 2.6 2 1 11 A_52_P393515 NM_146488 Olfr137 olfactory receptor 137 2.5 2 1 1 1 A_52_P449214 XM_357683 Gm1418 gene model 1418, (NCBI) 2.5 2 1 1 1 A_52_P303407 9430025N12 NA NA 2.5 2 1 1 1 A_51_P414746 NM_139222 Defb15 defensin beta 15 2.5 2 1 11 A_52_P263860 AK085051 D430030G11Rik RIKEN cDNA D430030G11 gene 2.5 2 1 1 1 A_51_P147445 AK050154 Lrrc28 leucine rich repeat containing 28 2.5 2 1 1 1 A_51_P222153 NM_009419 Tpst2 protein-tyrosine sulfotransferase 2 2.5 2 1 1 1 A_52_P1163666 AK032850 6720462K09Rik RIKEN cDNA 6720462K09 gene 2.5 2 1 1 1 A_51_P486569 NM_146385 Olfr1347 olfactory receptor 1347 2.5 2 1 1 1 A_51_P141211 NM_029570 Atp11b ATPase, Class VI, type 11B 2.5 2 1 11 A_51_P190124 NM_027347 Crsp3 cofactor required for Sp1 transcriptional activation, subunit 3 2.5 2 1 1 1 A_52_P97699 BC058677 D430019H16Rik RIKEN cDNA D430019H16 gene 2.5 2 1 11 A_52_P526417 AK034079 NA NA 2.5 2 1 1 1 A_52_P482724 BC042707 Prok1 prokineticin 1 2.5 2 1 11 A_51_P478774 AK033068 Chrna10 cholinergic receptor, nicotinic, alpha polypeptide 10 2.5 2 1 1 1 A_52_P453508 NM_010062 Dnase2a deoxyribonuclease II alpha 2.5 2 1 11 A_51_P344770 AK051922 Tnrc6b trinucleotide repeat containing 6b 2.5 2 1 1 1 A_51_P235687 NM_009663 Alox5ap arachidonate 5-lipoxygenase activating protein 2.5 221 1 A_52_P650379 NAP005800-001 NA NA 2.4 2 1 11 A_52_P536082 NM_027297 Prpf4 PRP4 pre-mRNA processing factor 4 homolog (yeast) 2.4 2 1 11 A_51_P417788 NM_008936 Prop1 paired like homeodomain factor 1 2.4 2 1 1 1 A_51_P208722 4733401I12 NA NA 2.4 2 1 1 1 A_52_P1100524 AK084052 NA NA 2.4 2 1 11 A_52_P218201 NM_026374 Ilf2 interleukin enhancer binding factor 2 2.4 2 1 1 1 A_52_P280832 NM_183035 Defb34 defensin beta 34 2.4 2 1 1 1 A_51_P324037 BG294955 NA NA 2.4 2 1 1 1 A_52_P158095 NAP107172-1 NA NA 2.4 2 1 11 A_51_P265465 NM_134130 Abhd3 abhydrolase domain containing 3 2.4 2 111 A_51_P268068 X80339 Six1 sine oculis-related homeobox 1 homolog (Drosophila) 2.4 1111 A_52_P418791 NM_011254 Rbp1 retinol binding protein 1, cellular 2.4 1111 A_52_P331930 NM_198937 D17Ertd441e DNA segment, Chr 17, ERATO Doi 441, expressed 2.4 2 1 11 A_52_P681016 AY344585 NA NA 2.4 2 1 1 1 A_51_P515120 NM_178870 Hs3st3a1 heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 2.4 1 112 Average Fold ProbeID Primary Accession Number Gene Symbol Gene Name Change Pair 1 Pair 1 Pair 2 Pair 2 A_51_P485405 AK005675 1700006H03Rik RIKEN cDNA 1700006H03 gene 2.4 2 111 A_51_P371279 NM_017480 Icos inducible T-cell co-stimulator 2.4 2 1 11 A_52_P456335 AK017955 MGI:1929864 melanocyte proliferating gene 1 2.4 2 111 A_52_P424308 NM_178765 5730410E15Rik RIKEN cDNA 5730410E15 gene 2.4 2 1 1 1 A_52_P477759 NM_008679 Ncoa3 nuclear receptor coactivator 3 2.4 2 1 1 1 A_51_P419439 NM_010321 Gnmt glycine N-methyltransferase 2.4 2 1 11 A_52_P1107462 AK020721 NA NA 2.4 2 111 A_51_P181097 XM_111232 Pfas phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) 2.4 2 1 1 1 A_51_P381784 BC052656 Exosc7 exosome component 7 2.4 2 1 1 1 A_52_P570690 NM_013415 Atp1b2 ATPase, Na+/K+ transporting, beta 2 polypeptide 2.4 2 1 1 1 A_51_P235619 AK041360 NA NA 2.4 2 1 1 1 A_51_P480020 AK016444 4931408D14Rik RIKEN cDNA 4931408D14 gene 2.4 2 111 A_52_P369705 NM_194343 Trim45 tripartite motif-containing 45 2.4 2 1 1 1 A_52_P383782 AK019394 3010027C24Rik RIKEN cDNA 3010027C24 gene 2.4 2 1 1 1 A_51_P351697 NM_025430 Mrpl35 mitochondrial ribosomal protein L35 2.4 2 1 1 1 A_51_P358700 NM_146902 Olfr1221 olfactory receptor 1221 2.4 2 1 1 1 A_51_P199249 NM_146839 Olfr272 olfactory receptor 272 2.4 1 1 1 NaN A_52_P641684 AK173173 Nfia nuclear factor I/A 2.4 2 1 1 1 A_52_P500418 NAP049824-1 NA NA 2.4 111 1 A_51_P337125 NM_010566 Inpp5d inositol polyphosphate-5-phosphatase D 2.4 2 1 1 1 A_52_P473542 AK016993 4933430H15Rik RIKEN cDNA 4933430H15 gene 2.4 2 1 1 1 A_51_P302527 AK077047 Nek1 NIMA (never in mitosis gene a)-related expressed kinase 1 2.4 2 1 1 1 A_51_P286780 BC028808 NA NA 2.4 2 1 11 A_51_P311904 NM_172498 Ptk2b PTK2 protein tyrosine kinase 2 beta 2.3 2 111 A_51_P200068 NM_007478 Arf3 ADP-ribosylation factor 3 2.3 2 111 A_52_P26409 M32070 NA NA 2.3 2 1 1 1 A_51_P369478 AK014554 Ppp2r5e protein phosphatase 2, regulatory subunit B (B56), epsilon isoform 2.3 2 1 1 1 A_51_P451416 A_51_P451416 NA NA 2.3 111 1 A_52_P584045 NM_146524 Olfr855 olfactory receptor 855 2.3 2 1 1 1 A_52_P135198 NM_177062 4833444G19Rik RIKEN cDNA 4833444G19 gene 2.3 2 111 A_52_P382754 NM_010875 Ncam1 neural cell adhesion molecule 1 2.3 2 1 11 A_52_P181394 BC096533 Grm5 glutamate receptor, metabotropic 5 2.3 111 1 A_52_P517174 AK030281 Wdr66 WD repeat domain 66 2.3 1 111 A_52_P324716 AK016951 4933427G17Rik RIKEN cDNA 4933427G17 gene 2.3 2 1 1 1 A_51_P170795 NM_178739 Wdr40b WD repeat domain 40B 2.3 1 1 NaN 1 A_51_P126959 NM_053219 V1ra4 vomeronasal 1 receptor, A4 2.3 2 1 1 1 A_52_P356886 AK015480 4930456L15Rik RIKEN cDNA 4930456L15 gene 2.3 2 111 A_52_P517668 A_52_P517668 NA NA 2.3 1 1 11 A_51_P103499 NM_025721 Spesp1 sperm equatorial segment protein 1 2.3 1 1 1 1 A_51_P448856 NM_019680 Elf4 E74-like factor 4 (ets domain transcription factor) 2.2 2 1 1 1 A_51_P431491 BC037132 Rab11fip3 RAB11 family interacting protein 3 (class II) 2.2 1111 A_51_P435348 NM_145586 8430420C20Rik RIKEN cDNA 8430420C20 gene 2.2 1 1 1 1 A_52_P514391 NM_009829 Ccnd2 cyclin D2 2.2 2 1 1 1 A_51_P457724 NM_147052 Olfr589 olfactory receptor 589 2.2 NaN 1 1 1 A_52_P396459 NM_009616 Adam19 a disintegrin and metalloproteinase domain 19 (meltrin beta) 2.2 1 111 A_52_P589468 NM_146444 Olfr458 olfactory receptor 458 2.2 111 1 A_52_P482299 XM_133692 Arhgef17 Rho guanine nucleotide exchange factor (GEF) 17 2.2 2 111 A_52_P148922 TC1508466 NA NA 2.2 1 1 11 A_51_P427934 XM_194337 Egfl4 EGF-like-domain, multiple 4 2.2 2 111 A_51_P471791 NM_145838 st8sia6 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6 2.2 1 1 1 1 A_51_P466367 NM_027667 Arhgap19 Rho GTPase activating protein 19 2.2 2 1 1 1 A_52_P524912 TC1507360 NA NA 2.2 1111 A_51_P434124 NM_029995 B430319H21Rik RIKEN cDNA B430319H21 gene 2.1 1 1 1 1 A_52_P106379 NAP106729-1 NA NA 2.1 1 1 1 1 A_52_P225117 D86419 Krtap6-1 keratin associated protein 6-1 2.1 1 1 1 1 A_51_P140263 NM_146820 Olfr655 olfactory receptor 655 2.1 1 1 1 1 A_52_P674414 NM_013791 Mkln1 muskelin 1, intracellular mediator containing kelch motifs 2.1 1 1 1 1 A_52_P639424 NM_173048 Gga3 golgi associated, gamma adaptin ear containing, ARF binding protein 3 2.1 1 1 1 1 A_51_P263983
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    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization I International Bureau (10) International Publication Number (43) International Publication Date WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT (51) International Patent Classification: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, C12Q 1/68 (2018.01) A61P 31/18 (2006.01) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C12Q 1/70 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (21) International Application Number: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PCT/US2018/056167 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 16 October 2018 (16. 10.2018) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 62/573,025 16 October 2017 (16. 10.2017) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, ΓΕ , IS, IT, LT, LU, LV, (71) Applicant: MASSACHUSETTS INSTITUTE OF MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TECHNOLOGY [US/US]; 77 Massachusetts Avenue, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Cambridge, Massachusetts 02139 (US).
  • Supporting Information

    Supporting Information

    Supporting Information The deubiquitinase USP38 promotes NHEJ repair through regulation of HDAC1 activity and regulates cancer cell response to genotoxic insults Yongfeng Yang1,2, Chuanzhen Yang1，2, Tingting Li3, Shuyu Yu1,2,Tingting Gan4, Jiazhi Hu4, Jun Cui5,6, and Xiaofeng Zheng1,2,* 1 State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China. 2Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing, China. 3State Key Laboratory of Proteomics, National Center of Biomedical Analysis, Institute of Basic Medical Sciences, Beijing, China. 4Department of Cell Biology, School of Life Sciences, Peking University, Beijing, China. 5Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. 6Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University, Guangzhou, China. *To whom correspondence should be addressed. Xiaofeng Zheng, School of Life Sciences, Peking University, Beijing 100871, China. Tel: +86 10-6275-5712; Email: [email protected] Supplementary Materials and Methods: Antibodies and reagents Mouse monoclonal anti-Flag (F3165, RRID: AB_259529) antibodies, anti-HA (H9658, RRID: AB_260092) antibodies, and etoposide (E1383) were purchased from Sigma-Aldrich. Mouse monoclonal anti-Myc (M047-3, RRID: AB_591112), anti-histidine (D291–3, RRID: AB_10597733), and rabbit polyclonal anti-β-actin (PM053, RRID: AB_10598196) antibodies were purchased from MBL. Mouse monoclonal anti-H3 (BE3015) antibodies were purchased from EASYBIO. Mouse monoclonal anti-γH2AX (05–636, RRID: AB_309864) antibodies and MG132 (2772605) were purchased from Millipore. Mouse monoclonal anti-HDAC1 (sc-81598, RRID: AB_2118083) antibodies were purchased from Santa Cruz Biotechnology.
  • The Galanin and Galanin Receptor Subtypes, Its Regulatory Role in the Biological and Pathological Functions

    The Galanin and Galanin Receptor Subtypes, Its Regulatory Role in the Biological and Pathological Functions

    Physiol. Res. 66: 729-740, 2017 REVIEW The Galanin and Galanin Receptor Subtypes, its Regulatory Role in the Biological and Pathological Functions J. ŠÍPKOVÁ1, I. KRAMÁRIKOVÁ1, S. HYNIE1, V. KLENEROVÁ1 1Laboratory of Neuropharmacology, Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University, Prague, Czech Republic Received December 16, 2016 Accepted March 31, 2017 On-line July 18, 2017 Summary Corresponding author The multitalented neuropeptide galanin was first discovered V. Klenerová, Laboratory of Neuropharmacology, Institute of 30 years ago but initially no biologic activity was found. Further Medical Biochemistry and Laboratory Diagnostics, First Faculty of research studies discovered the presence of galanin in the brain Medicine, Charles University, Albertov 4, 128 00 Prague 2, Czech and some peripheral tissues, and galanin was identified as Republic. Tel/Fax: +420 224 968 166, +420 224 968 142. a modulator of neurotransmission in the central and peripheral E-mail: [email protected] nervous system. Over the last decade there were performed very intensive studies of the neuronal actions and also of nonneuronal Introduction actions of galanin. Other galanin family peptides have been described, namely galanin, galanin-like peptide, galanin-message The galaninergic system is one of the specific associated peptide and alarin. The effect of these peptides is signaling systems involved in neurotransmission and mediated through three galanin receptors subtypes, GalR1, GalR2 neuromodulation. The principal molecule of this system and GalR3 belonging to G protein coupled receptors, and is the neuropeptide galanin. Galanin molecule was firstly signaling via multiple transduction pathways, including inhibition described more than thirty years ago in the porcine of cyclic AMP/protein kinase A (GalR1, GalR3) and stimulation of intestine (Tatemoto et al.