Supplement 2. Tables.

Table 1. Primers used for real-time PCR analysis.

Gene Primer ID* AZGP 4502337a2 Bcl2-related A1 (Bcl2-A1) 4757840a3 carboxypeptidase A4 10047106a3 carcinoembryonic antigen-related cell adhesion 11386171a2 molecule 5 cathepsin B 22538437a3 CD10 4505203a3 CD46 27502402a3 CD55 10835143a3 Cdc25C 4502707a3 chemokine (C-C motif) ligand 20 (CCL20) 4759076a1 cyclin B1 14327896a3 cyclooxygenase-2 (COX-2) 4506265a2 cystein rich protein Cyr61 4128147a1 type II/K6HF 4758618a1 farnesyltransferase β 10835059a3 galectin 4504983a3 glucose-regulated protein 78kDa, GRP78 16507237a1 hyaluronan synthase 4885391a3 IL-13 receptor A2 10834992a3 IL-1β 10835145a3 IL-24 5803086a1 IL-6 10834984a2 integrin β4 6453380a1 kallikrein 5 11244763a2 17 4557701a1 24234699a1 15431316a1 Mac-2 binding protein 5031863a3 matrix Gla protein 4505179a1 p21/Cip1 11386203a1 perlecan 184425a3 plasminogen activator inhibitor-2 (PAI-2) 4505595a1 ribosomal protein S25 (RPS25) 4506707a1 thymidylate kinase 6912340a1 TNF-β induced protein 2 26051240a3 tryptase-ε/serin protease 22 11545839a2 tumor necrosis factor ligand superfamily, member 9 4507609a1 tumor necrosis factor-α inducible protein A20 5454132a3

Following primers were used to analyze : 5’ CACCGTCTCCAGTGGCTATG 3’ and 5’ GAACTAAAGCCGCCTCCAAC 3’

*Ref.: Wang X, Seed B. A PCR primer bank for quantitative expression analysis. Nucleic Acids Res 2003;31:e154.

Table 2. Common up-regulated expressed at 72 h of TB and MPA treatment.

Gene Accession # TB 72 h MPA 72 h S100 calcium binding protein A8 (calgranulin A) NM_002964 21.8 6.2 keratin, hair, acidic, 4 NM_021013 10.3 3.6 complement component 3 NM_000064 9.2 5.3 L1 cell adhesion molecule AI653981 9.1 5.8 cytokeratin type II NM_004693 7.3 11.4 zinc finger protein 83 (HPF1) M27877 6.2 3.5 normal mucosa of specific 1 AF228422 5.3 6.4 protease, serine, 22 NM_022119 5.0 3.5 , alpha 3 AF141347 5.0 2.1 tumor protein p53 inducible nuclear protein 1 AW341649 4.9 3.9 family member 5C NM_004522 4.9 4.5 collagen, type VI, alpha 1 AI141603 4.9 2.2 spermidine/spermine N1-acetyltransferase BE326919 4.7 4.4 serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 AL574210 4.7 3.0 tumor necrosis factor, alpha-induced protein 2 NM_006291 4.5 2.1 interferon induced transmembrane protein 1 (9-27) AA749101 4.2 2.1 2',5'-oligoadenylate synthetase 1, 40/46kDa NM_002534 4.0 2.6 RAB3B, member RAS oncogene family AU156710 3.9 5.0 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) binding protein 1 NM_017870 3.8 2.4 growth differentiation factor 15 AF003934 3.8 4.2 E74-like factor 3 (ets domain transcription factor, epithelial- specific ) U73844 3.8 3.8 N-myc downstream regulated gene 1 NM_006096 3.8 2.9 trophoblast-derived noncoding RNA AI042152 3.8 4.2 golgi SNAP receptor complex member 2 AW205003 3.6 4.7 major vault protein NM_017458 3.5 2.2 calcium/calmodulin-dependent protein kinase II NM_018584 3.4 2.3 pleckstrin homology domain containing, family B (evectins) member 1 AF081583 3.4 2.3 molecule possessing repeats induced by lipopolysaccharide (MAIL), homolog of mouse AB037925 3.2 3.8 netrin 4 AF278532 3.2 2.5 trophoblast-derived noncoding RNA AV699657 3.2 2.3 keratin associated protein 2-1 BC012486 3.1 6.3 lipocalin 2 (oncogene 24p3) NM_005564 3.1 2.7 trophoblast-derived noncoding RNA BE675516 3.1 3.4 interferon, alpha-inducible protein 27 NM_005532 3.1 2.3 -associated protein 1B AA554833 3.0 2.4 sterile alpha motif domain containing 4 BF697247 3.0 3.3 NEDD9 interacting protein with calponin homology and LIM domains NM_022765 2.9 2.4 amyloid beta (A4) precursor-like protein 1 U48437 2.9 2.2 protease inhibitor 3, skin-derived (SKALP) NM_002638 2.9 2.3 p8 protein (candidate of metastasis 1) AF135266 2.9 2.4 kinesin family member C2 BF515031 2.8 8.7 gamma-aminobutyric acid (GABA) B receptor, 1 NM_001470 2.8 2.3 microtubule-associated protein 1B AL523076 2.8 3.3 sterile alpha motif domain containing 4 AB028976 2.8 2.8 , heavy polypeptide 14 NM_024729 2.8 4.5 kallikrein 5 AF243527 2.7 4.1 interferon-stimulated transcription factor 3, gamma 48kDa NM_006084 2.7 4.3 myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse) NM_002462 2.7 2.2 neural precursor cell expressed, developmentally down- regulated 9 U64317 2.7 2.1 protein phosphatase 1, regulatory (inhibitor) subunit 12C AF312028 2.7 3.4 ferritin, heavy polypeptide 1 AA083483 2.6 4.1 dual specificity phosphatase 10 N36770 2.6 4.1 pim-1 oncogene M24779 2.6 2.3 xanthine dehydrogenase BG260086 2.6 4.5 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 AF009670 2.6 2.8 angiomotin like 2 NM_016201 2.6 2.3 fatty acid desaturase 3 NM_021727 2.6 2.1 two pore segment channel 1 NM_017901 2.5 2.4 angiopoietin-like 4 NM_016109 2.5 2.3 glucocorticoid induced transcript 1 AA058770 2.4 3.6 adult retina protein AA481560 2.4 2.8 zinc finger protein 432 NM_014650 2.4 3.7 glutamate decarboxylase 1 (brain, 67kDa) NM_000817 2.4 3.6 major histocompatibility complex, class I, E X56841 2.4 2.3 lectin, galactoside-binding, soluble, 3 binding protein NM_005567 2.4 2.1 brain expressed X-linked-like 1 AL523320 2.4 2.4 fibronectin 1 X02761 2.4 3.7 cystathionase (cystathionine gamma-lyase) AL354872 2.4 2.2 bone morphogenetic protein 2 NM_001200 2.3 3.2 cathepsin B W47179 2.3 2.0 glucocorticoid induced transcript 1 AC006042 2.3 3.9 ATPase, Class I, type 8B, member 2 AB032963 2.3 2.5 optineurin AV757675 2.3 2.2 major histocompatibility complex, class I, C L07950 2.3 2.2 golgin-67 AF163441 2.2 2.8 jun dimerization protein 2 AA716425 2.2 2.2 calpain 13 AK026692 2.2 2.1 sestrin 2 AL136551 2.2 2.8 F11 receptor AI814545 2.2 2.1 (proline 4-hydroxylase), alpha polypeptide II NM_004199 2.2 2.1 laminin, beta 3 L25541 2.2 3.9 zinc finger, FYVE domain containing 1 AB046809 2.2 2.4 transmembrane protease, serine 4 NM_016425 2.2 5.7 tectorin alpha NM_005422 2.2 2.5 solute carrier family 6 (neurotransmitter transporter),member 16 BC039336 2.2 3.8 protein phosphatase 2C, magnesium-dependent, catalytic subunit NM_018444 2.2 3.1 UDP-glucose ceramide glucosyltransferase W72338 2.2 2.2 trans-golgi network protein 2 BC028219 2.1 3.8 laminin, beta 2 (laminin S) X79683 2.1 3.5 TBC1 domain family, member 3 AL136860 2.1 2.8 mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N- acetylglucosaminyltransferase, isoenzyme A AW006441 2.1 2.0 AI922599 2.0 2.7 tubulin, beta polypeptide paralog AL533838 2.0 2.2 ras homolog gene family, member E BG054844 2.0 2.0 kynureninase (L-kynurenine hydrolase) BC000879 2.0 3.6

Table 3. Common down-regulated genes at 12 h of TB and MPA treatment.

Gene Accession # T12 M12 hyaluronan synthase 2 NM_005328 -9.8 -3.1 B1 NM_005573 -4.2 -2.1 cyclin B1 N90191 -3.2 -2.1 SMAD, mothers against DPP homolog 1 (Drosophila) U54826 -2.9 -2.4 basic helix-loop-helix domain containing, class B, 3 AI819798 -2.8 -3.1 splicing factor, arginine/serine-rich, 46kD NM_032102 -2.8 -2.2 thymopoietin AF113682 -2.8 -2.7 nuclear receptor subfamily 2, group F, member 1 NM_005654 NM_005654 -2.3 -2.0 zinc finger protein 599 BC033354 -2.3 -2.1 basic helix-loop-helix domain containing, class B, 3 NM_030762 -2.2 -2.4 farnesyltransferase, CAAX box, beta NM_002028 -2.2 -2.5 hemicentin-2 AW778829 -2.2 -2.2 PHD finger protein 14 NM_014660 -2.1 -3.5 hemochromatosis type 2 (juvenile) AA948096 -2.1 -2.5 arginine vasopressin receptor 2 (nephrogenic diabetes insipidus) NM_000054 -2.1 -2.2 topoisomerase (DNA) II alpha 170kDa NM_001067 -2.1 -2.1 nuclear receptor subfamily 2, group C, member 2 AL138444 -2.0 -2.6

Table 4. Common down-regulated proliferation genes at 72 h of TB and MPA treatment.

Gene Accession # TB 72h MPA 72h polo-like kinase 4 (Drosophila) NM_014264 Z25433 -8.2 -3.1 cell division cycle associated 1 AF326731 -7.7 -2.1 cell division cycle 25A AY137580 -7.7 -2.4 CHK1 checkpoint homolog (S. pombe) NM_001274 -7.7 -2.2 CDC6 cell division cycle 6 homolog (S. cerevisiae) NM_001254 -7.4 -2.1 DNA replication complex GINS protein PSF2 BC003186 -7.4 -3.4 MAD2 mitotic arrest deficient-like 1 (yeast) AF394735 -7.4 -2.3 cyclin A2 NM_001237 -7.3 -2.8 replication factor C (activator 1) 3, 38kDa NM_002915 -7.3 -2.0 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) AI859865 -7.2 -2.4 kinetochore associated 2 NM_006101 -6.8 -2.0 condensation protein G NM_022346 -6.2 -2.9 antigen identified by monoclonal antibody Ki-67 AU132185 -6.1 -2.1 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) NM_006739 -6.0 -3.3 G-2 and S-phase expressed 1 NM_016426 -5.8 -2.0 S-phase kinase-associated protein 2 (p45) BC001441 -5.6 -6.9 serine/threonine kinase 6 NM_003158 -5.4 -2.2 antigen identified by monoclonal antibody Ki-67 BF001806 -5.3 -2.4 NM_004523 -5.3 -2.7 baculoviral IAP repeat-containing 5 (survivin) AA648913 -5.3 -2.3 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) AF043294 -5.2 -3.1 kinesin family member 2C AY026505 -5.2 -2.1 NIMA (never in gene a)-related kinase 2 NM_002497 -5.0 -2.3 kinesin-like 7 NM_020242 -5.0 -2.1 CDC20 cell division cycle 20 homolog (S. cerevisiae) NM_001255 -5.0 -2.1 cyclin B1 N90191 -4.9 -3.0 insulin-like growth factor binding protein 4 NM_001552 -4.8 -6.1 SMC2 structural maintenance of 2-like 1 (yeast) NM_006444 -4.7 -2.3 cell division cycle 25C NM_001790 -4.5 -3.0 deoxythymidylate kinase (thymidylate kinase) NM_012145 -4.1 -2.8 high-mobility group box 1 NM_002128 N92507 -4.1 -4.6 cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) NM_001262 -4.1 -4.3 chromatin assembly factor 1, subunit A (p150) NM_005483 -4.0 -6.2 MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) NM_005915 -3.9 -2.0 CDC45 cell division cycle 45-like (S. cerevisiae) NM_003504 -3.9 -3.4 polymerase (DNA directed), epsilon 3 (p17 subunit) BC004170 -3.9 -2.7 transcription factor Dp-1 NM_007111 AI950069 -3.8 -4.2 parathyroid hormone-like hormone BC005961 -3.7 -3.4 chromosome condensation-related SMC-associated protein 1 AK022511 -3.6 -2.3 M-phase phosphoprotein 1 NM_016195 -3.5 -2.1 cell division cycle 2, G1 to S and G2 to M AA749427 -3.4 -2.3 glioma-amplified sequence-41 NM_006530 -3.4 -2.2 CHK1 checkpoint homolog (S. pombe) NM_001274 -3.3 -2.6 kinesin family member 22 AC002301 -3.2 -2.4 polo-like kinase 1 (Drosophila) NM_005030 -3.0 -2.1 primase, polypeptide 2A, 58kDa NM_000947 -3.0 -2.0 M-phase phosphoprotein 9 NM_022782 -3.0 -2.2 transcription factor A, mitochondrial BE552470 -2.9 -3.0 CSE1 chromosome segregation 1-like (yeast) AF053640 -2.8 -2.0 Nijmegen breakage syndrome 1 (nibrin) AF049895 -2.8 -2.4 origin recognition complex, subunit 5-like (yeast) NM_002553 -2.5 -2.2 interleukin enhancer binding factor 3, 90kDa BG032366 -2.5 -3.3 hepatoma-derived growth factor, related protein 3 BE703418 -2.4 -2.1 chromosome condensation 1 NM_001269 -2.4 -2.7 cell division cycle 25C AF277724 -2.4 -2.4 SET translocation (myeloid leukemia-associated) Z95126 -2.3 -4.0 Wilms tumor 1 NM_024426 -2.3 -2.7 chondroitin sulfate proteoglycan 6 (bamacan) AI373676 -2.3 -2.6 polybromo 1 AF197569 -2.3 -2.2 RAN, member RAS oncogene family BF112006 -2.2 -2.0 cullin 2 U83410 -2.2 -2.0 RNA binding motif protein 14 NM_006328 -2.2 -2.2 stromal antigen 1 AW168948 -2.2 -2.2 deleted in lymphocytic leukemia, 1 NM_005887 -2.2 -3.0 putative endoplasmic reticulum multispan transmembrane protein BF475369 -2.2 -2.7 sterile alpha motif and leucine zipper containing kinase AZK AW069181 -2.2 -3.0 replication factor C (activator 1) 5, 36.5kDa BG260658 -2.2 -2.2 proliferation-associated 2G4, 38kDa AL136460 -2.0 -2.3