DOCSLIB.ORG

International Intracellular Bacteria Meeting 2022

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
International Intracellular Bacteria Meeting 2022 International intracellular bacteria meeting 2022 Joint ESCCAR International congress on Rickettsiae and 9th Meeting of the European Society for Chlamydia Research (ESCR) August, 23-26, 2022 Auditoire César Roux University Hospital – CHUV Lausanne, Switzerland Organizing Societies American Rickettsia Society European Society for Chlamydia Research European Society on intracellular bacteria (ESCCAR) Local Scientifi c and Organizing Committee Greub Gilbert Aeby Sébastien Ardissone Silvia Jacquier Nicolas Kebbi-Beghdadi Carole Institut de Microbiologie Université de Lausanne – CHUV Congress offi ce Management Meeting.com Congress Organisation Rue des Pâquis 1 • CH-1033 Cheseaux-sur-Lausanne www.meeting-com.ch • [email protected] • T 021 312 92 61 © Régis Colombo / www.diapo.ch PROGRAM ORGANIZING SOCIETIES EUROPEAN STUDY GROUP ON American Society for Rickettsiology (ASAR) ESGMD Executive Committee President Kevin Macaluso, Baton Rouge, LA Chair Gilbert Greub, Switzerland Vice-President Joao Pedra, Baltimore, MD Secretary Hege Vangstein Aamot, Norway Treasurer Daniel E. Voth, Little Rock, AR Treasurer Frederic Laurent, France Councilor-at-large Alison Fedrow, West Newton, PA Education Officer Amel Renaud Bidias, Cameroon Past President Kelly Brayton, Pullman, WA ESGAMC Executive Committee European Society for Chlamydia Research (ESCR) Chair Ran Nir-Paz, Israël Chair Gilbert Greub, Switzerland Secretary Cecile Bébéar, France Co-Chair Patrick Horner, United Kingdom Treasurer Gilbert Greub, Switzerland Board Member Servaas Morré, The Netherlands Education Officer Katherine Loens, Belgium Board Member Angelika Stari, Austria Science Officer Michael Beeton, United Kingdom Board Member Per-Anders Mårdh, Finland Committees Board Member Matthias Maas, Austria Committees Board Member Judit Deàk, Hungary Board Member Konrad Sachse, Germany AWARDS COMMITTEE European Society on Intracellular Bacteria (ESCCAR) President Gilbert Greub, Switzerland Best Poster Award Pierre-Edouard Fournier, France Vice-president Aranzazu Portillo Barrio, Spain Carole Kebbi-Beghdadi, Switzerland Past-president Pierre-Edouard Fournier, France Beate Henrichfreise, Germany Secretary Idir Bitam, Algeria Best Oral Award Nicolas Jacquier, Switzerland Board member Chantal Bleeker-Rovers, The Netherlands Chlamydia Servaas Morré, The Netherlands Board member Mirja Puollakainen, Finland Mirja Puollakainen, Finland Board member Beate Henrichfreise, Germany Best Oral Award José A. Oteo, Spain Rickettsia Daniel Paris, Switzerland Aranzazu Portillo Barrio, Spain Best Oral Award Chantal Bleeker-Rovers, The Netherlands Others Max Maurin, France Ran Nir-Paz, Israël 2 3 Dear Colleagues, Tuesday, August 23th The situation of the COVID-19 requires a review of the organization on a day- to-day basis, both in our usual work than in the planned meetings. Therefore – as you will easily understand – we have taken the decision to postpone the 08.00 Registration FOYER international intracellular bacteria meeting once again, to August 23-26, 2022, 08.50 Welcome word at the CHUV in Lausanne. 09.00-10.30 SESSION 1 CÉSAR ROUX This meeting is a joint meeting between the ESCCAR meeting (European KEYNOTES Society on Chlamydia, Coxiella, Anaplasma and Rickettsia), that was held Chairs : Aranzazu Portillo Barrio, La Rioja (ES) ; in Marseille in 2018 and the meeting of the European Society for Program Gilbert Greub, Lausanne (CH) Chlamydia Research, that took place in 2016 in Oxford. 09.00-09.45 Chlamydia is a Master Cell Biologist : Welcome The meeting is co-organized by the ESCCAR board, the European chlamydia The Secret Life of an Intracellular Pathogen board and the American rickettsial society. It is also supported by the Joanne Engel, San Francisco (US) ESGMD (genomics and molecular diagnostics) and ESMAC (mycoplasma 09.45-10.30 Rickettsia : lessons from genomics and chlamydia) groups of ESCMID (European Society of Clinical Microbiology Pierre-Edouard Fournier, Marseille (FR) and Infectious Diseases). As usual, we will have dedicated sessions and talks on various aspects 10.30-11.00 Coffee break FOYER including epidemiology, genomics, cell biology, molecular biology, diagnostics & infection management of a variety of bacterial agents, including 11.00-12.30 SESSION 2 A CÉSAR ROUX Anaplasma, Bartonella, Chlamydia, Coxiella, Ehrlichia, Rickettsia, Waddlia HOST-CELL INTERACTIONS and Wolbachia. Chairs : Christine Suetterlin, Irvine (US) ; Looking forward to welcoming you in Lausanne. Anders Omsland, Pullman (US) Best regards, 11.00-11.30 Manipulation of host cholesterol in the bacterial intracellular niche Stacey D. Gilk, Indianapolis (US) 11.30-12.00 Anaplasma : cell corruption by an intracellular bacteria J. Stephen Dumler, Bethesda (US) Professeur Gilbert GREUB, MD-PhD 12.00-12.15 Short talk Institute of Microbiology, UNIL-CHUV, Lausanne 12.15-12.30 Short talk 4 5 Tuesday, August 23th Tuesday, August 23th 11.00-12.30 SESSION 2 B AUGUSTE TISSOT 14.00-15.30 SESSION 3 B AUGUSTE TISSOT EPIDEMIOLOGY OF AGENTS CHLAMYDIA HOST CELL INTERACTIONS OF ATYPICAL PNEUMONIA Chairs : Jeanne Salje, Newark (US) ; Chairs : Cécile Bebear, Bordeaux (FR) ; Antony Croxatto, Lausanne (CH) Ran Nir-Paz, Jerusalem (IL) 11.00-11.30 Mycoplasma pneumoniae infections across Europe 14.00-14.30 Effect of Chlamydia on centrosome and cilia and Israel (2011-2016) Christine Suetterlin, Irvine (US) Program Michael Beeton, Cardiff (GB) Program 14.30-15.00 The type three secretion system 11.30-12.00 Co-circulation within a network structured of Waddlia chondrophila population induce cycling epidemics Carole Kebbi-Beghdadi, Lausanne (CH) Vicky Chalker, London (GB) 15.00-15.15 Short talk 12.00-12.15 Short talk 15.15-15.30 Short talk 12.15-12.30 Short talk 15.30-16.00 Coffee break FOYER 12.30-14.00 Lunch time and posters (even numbers <200) FOYER 16.00-17.30 SESSION 4 CÉSAR ROUX 12.30-13.30 Closed ESCCAR Committee Meeting SEMINAR ROOM 3 MOLECULAR PATHOGENESIS Chairs : Joanne Engel, San Francisco (US) ; 14.00-15.30 SESSION 3 A CÉSAR ROUX Jaime Mota, Caparica (PT) DIAGNOSIS 16.00-16.30 Molecular pathogenesis of chlamydial infection Chairs : Hege Vangstein Aamot, Lorenskog (NO) ; Raphaël Valdivia, Durham (US) Onya Opota, Lausanne (CH) 16.30-17.00 Molecular pathogenesis of Bartonella infection 14.00-14.30 Diagnosis of rickettsial infections Christoph Dehio, Basel (CH) Didier Raoult, Marseille (FR) 17.00-17.15 Short talk 14.30-15.00 Q fever : what’s new ? Chantal Bleeker-Rovers, Nijmegen (NL) 17.15-17.30 Short talk 15.00-15.15 Short talk 15.15-15.30 Short talk 18.00-20.00 KROBS EVENING SEMINAR ROOMS 3-4 6 7 Wednesday, August 24th Wednesday, August 24th 11.00-12.30 SESSION 6 A CÉSAR ROUX 07.00-08.00 Running from Croisettes (Epalinges, 6-7 km) BACTERIAL DIVISION AND PEPTIDOGLYCAN Chairs : Beate Henrichfreise, Bonn (DE) ; 08.00 Registration FOYER Raphaël Valdivia, Durham (US) 11.00-11.30 Peptidoglycan of chlamydia 09.00-10.30 SESSION 5 A CÉSAR ROUX Georges Liechti, Bethesda (US) MOLECULAR MICROBIOLOGY AND BACTERIAL 11.30-12.00 Chlamydial division GENETICS Program Program Nicolas Jacquier, Lausanne (CH) Chairs : Deborah Dean, Oakland (US) ; Servaas Morré, Maastricht (NL) 12.00-12.15 Short talk 12.15-12.30 Short talk 09.00-09.30 Genetic dissection of chlamydial infection biology Ken Fields, Lexington (US) 11.00-12.30 SESSION 6 B AUGUSTE TISSOT 09.30-10.00 Transcriptional regulation of Chlamydia MOLECULAR DIAGNOSIS OF INTRACELLULAR Ming Tan, Irvine (US) BACTERIA 10.00-10.15 Short talk Chairs : Victoria Jane Chalker, London (GB) ; Michael Beeton, Cardiff (GB) 10.15-10.30 Short talk 11.00-11.30 How to develop a new diagnostic PCR to detect intracellular bacteria ? Kate Templeton, Edinburgh (GB) 09.00-10.30 SESSION 5 B AUGUSTE TISSOT FROM THE BENCH TO BEDSIDE 11.30-12.00 Added value of PCR for diagnosis of atypical pneumonia Chairs : Mirja Puolakkainen, Helsinki (FI) ; Katherine Loens, Antwerp (BE) Paul Newton, Ventiane (US) 12.00-12.15 Short talk 09.00-09.30 Scrub typhus : an overview 12.15-12.30 Short talk Daniel Paris, Basel (CH) 12.30-14.00 Lunch time and Posters (even numbers >200) FOYER 09.30-10.00 Typhus and spotted fever : clinical overview Amel Letaïf, Sousse (TN) 12.30-13.00 Committee of European Chlamydia Society SEMINAR ROOM 3 10.00-10.15 Short talk 10.15-10.30 Short talk 14.00-15.30 SESSION 7 A CÉSAR ROUX GENOMICS Chairs : Linda Mueller, Lausanne (CH) ; 10.30-11.00 Coffee break FOYER Ted Hackstadt, Hamilton (US) 14.00-14.30 Lessons from Chlamydia trachomatis genomics Deborah Dean, Oakland (US) 14.30-15.00 Genomics of Chlamydia-related bacteria Trestan Pillonel, Lausanne (CH) 15.00-15.15 Short talk 15.15-15.30 Short talk 8 9 Wednesday, August 24th Thursday, August 25th 14.00-15.30 SESSION 7 B AUGUSTE TISSOT CELL & MOLECULAR BIOLOGY 07.00-08.00 Running from Ouchy (lakeshore, 6-7 km) OF OTHER INTRACELLULAR BACTERIA Chairs : Helena Seth-Smith, Basel (CH) ; 08.00 Registration FOYER Steven Ball, Lille (FR) 09.00-10.30 SESSION 9 A CÉSAR ROUX 14.00-14.30 The developmental cycle of Orientia tsutsugamushi CELL BIOLOGY Jeanne Salje, Newark (US) Chairs : Elizabeth Rucks, Omaha (US) ; Program 14.30-15.00 Formation of a pathogen vacuole : how Legionella Program Christoph Dehio, Basel (CH) pneumophila kills one bird with many stones 09.00-09.30 Rickettsia/host-cell interactions Hubert Hilbi, Zurich (CH) Ted Hackstadt, Hamilton (US) 15.00-15.15 Short talk 09.30-10.00 Rickettsia typhi Subverts Intracellular Trafficking 15.15-15.30 Short talk to Establish Replicative Niche Mohammed Sayeedur Rahman, Baltimore (US) 15.30-16.00 Coffee break FOYER 10.00-10.15
Load more

Recommended publications
  • University of Zurich Posted at the Zurich Open Repository and Archive, University of Zurich
    Bertelli, C; Collyn, F; Croxatto, A; Rückert, C; Polkinghorne, A; Kebbi-Beghdadi, C; Goesmann, A; Vaughan, L; Greub, G (2010). The Waddlia genome: a window into chlamydial biology. PloS one, 5(5):e10890. Postprint available at: http://www.zora.uzh.ch University of Zurich Posted at the Zurich Open Repository and Archive, University of Zurich. Zurich Open Repository and Archive http://www.zora.uzh.ch Originally published at: PloS one 2010, 5(5):e10890. Winterthurerstr. 190 CH-8057 Zurich http://www.zora.uzh.ch Year: 2010 The Waddlia genome: a window into chlamydial biology Bertelli, C; Collyn, F; Croxatto, A; Rückert, C; Polkinghorne, A; Kebbi-Beghdadi, C; Goesmann, A; Vaughan, L; Greub, G Bertelli, C; Collyn, F; Croxatto, A; Rückert, C; Polkinghorne, A; Kebbi-Beghdadi, C; Goesmann, A; Vaughan, L; Greub, G (2010). The Waddlia genome: a window into chlamydial biology. PloS one, 5(5):e10890. Postprint available at: http://www.zora.uzh.ch Posted at the Zurich Open Repository and Archive, University of Zurich. http://www.zora.uzh.ch Originally published at: PloS one 2010, 5(5):e10890. The Waddlia Genome: A Window into Chlamydial Biology Claire Bertelli1., Franc¸ois Collyn1., Antony Croxatto1, Christian Ru¨ ckert2, Adam Polkinghorne3,4, Carole Kebbi-Beghdadi1, Alexander Goesmann2, Lloyd Vaughan4, Gilbert Greub1* 1 Center for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland, 2 Center for Biotechnology, Bielefeld University, Bielefeld, Germany, 3 Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia, 4 Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland Abstract Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants.
    [Show full text]
  • Experimental Challenge of Pregnant Cattle with the Putative Abortifacient
    Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila Author List Nicholas Wheelhouse1# & *, Allen Flockhart1# &, Kevin Aitchison1, Morag Livingstone1, Jeanie Finlayson1, Virginie Flachon2, Eric Sellal2, Mark P. Dagleish1 & David Longbottom1 Affiliations 1 Moredun Research Institute, Edinburgh, Midlothian, United Kingdom. 2 Biosellal, 317 Avenue Jean Jaurès, 69007 Lyoncedex, France # Current address: School of Life, Sport and Social Sciences, Edinburgh Napier University, Sighthill Campus, Edinburgh EH11 4BN, United Kingdom *Corresponding author: E:mail: [email protected] &These authors contributed equally to this manuscript 1 Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct experimental evidence exists. Given this paucity of direct evidence the current study was carried out to investigate whether experimental challenge of pregnant cattle with W. chondrophila would result in infection and abortion. Nine pregnant Friesian-Holstein heifers received 2 x 108 inclusion forming units (IFU) W. chondrophila intravenously on day 105-110 of pregnancy, while four negative-control animals underwent mock challenge. Only one of the challenged animals showed pathogen-associated lesions, with the organism being detected in the diseased placenta. Importantly,
    [Show full text]
  • INFECTIOUS DISEASES of HAITI Free
    INFECTIOUS DISEASES OF HAITI Free. Promotional use only - not for resale. Infectious Diseases of Haiti - 2010 edition Infectious Diseases of Haiti - 2010 edition Copyright © 2010 by GIDEON Informatics, Inc. All rights reserved. Published by GIDEON Informatics, Inc, Los Angeles, California, USA. www.gideononline.com Cover design by GIDEON Informatics, Inc No part of this book may be reproduced or transmitted in any form or by any means without written permission from the publisher. Contact GIDEON Informatics at [email protected]. ISBN-13: 978-1-61755-090-4 ISBN-10: 1-61755-090-6 Visit http://www.gideononline.com/ebooks/ for the up to date list of GIDEON ebooks. DISCLAIMER: Publisher assumes no liability to patients with respect to the actions of physicians, health care facilities and other users, and is not responsible for any injury, death or damage resulting from the use, misuse or interpretation of information obtained through this book. Therapeutic options listed are limited to published studies and reviews. Therapy should not be undertaken without a thorough assessment of the indications, contraindications and side effects of any prospective drug or intervention. Furthermore, the data for the book are largely derived from incidence and prevalence statistics whose accuracy will vary widely for individual diseases and countries. Changes in endemicity, incidence, and drugs of choice may occur. The list of drugs, infectious diseases and even country names will vary with time. © 2010 GIDEON Informatics, Inc. www.gideononline.com All Rights Reserved. Page 2 of 314 Free. Promotional use only - not for resale. Infectious Diseases of Haiti - 2010 edition Introduction: The GIDEON e-book series Infectious Diseases of Haiti is one in a series of GIDEON ebooks which summarize the status of individual infectious diseases, in every country of the world.
    [Show full text]
  • Thesis Reference
    Thesis Targeting Waddlia chondrophila development cycle using genetic tools and metabolomics WALTER, Chantal Abstract All members of the Chlamydiae, including Waddlia chondrophila share the same unique intracellular development cycle, which is the focus of this work in the search for anti-chlamydial targets and drugs. Strategies using high throughput screening, genetic complementation, and metabolomics were explored to investigate and try to block the W. chondrophila development cycle. First, a generic high throughput screening procedure was developed in a heterologous system, Escherichia coli, to search for inhibitors of chlamydial transcription factors. Then, the function of DksA, a transcription factor, was investigated by genetic complementation of E. coli and Pseudomonas aeruginosa mutants. Finally, metabolomics was adopted to observe changes in metabolites associated with progress through the chlamydial developmental cycle. It revealed the presence of putative novel metabolites differentially expressed in the various stages. Altogether, the results of this work present putative new therapeutic approaches to understand and circumvent the infection process of this pathogen. Reference WALTER, Chantal. Targeting Waddlia chondrophila development cycle using genetic tools and metabolomics. Thèse de doctorat : Univ. Genève, 2019, no. Sc. 5337 DOI : 10.13097/archive-ouverte/unige:119059 URN : urn:nbn:ch:unige-1190597 Available at: http://archive-ouverte.unige.ch/unige:119059 Disclaimer: layout of this document may differ from the published
    [Show full text]
  • International Code of Nomenclature of Prokaryotes
    2019, volume 69, issue 1A, pages S1–S111 International Code of Nomenclature of Prokaryotes Prokaryotic Code (2008 Revision) Charles T. Parker1, Brian J. Tindall2 and George M. Garrity3 (Editors) 1NamesforLife, LLC (East Lansing, Michigan, United States) 2Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany) 3Michigan State University (East Lansing, Michigan, United States) Corresponding Author: George M. Garrity ([email protected]) Table of Contents 1. Foreword to the First Edition S1–S1 2. Preface to the First Edition S2–S2 3. Preface to the 1975 Edition S3–S4 4. Preface to the 1990 Edition S5–S6 5. Preface to the Current Edition S7–S8 6. Memorial to Professor R. E. Buchanan S9–S12 7. Chapter 1. General Considerations S13–S14 8. Chapter 2. Principles S15–S16 9. Chapter 3. Rules of Nomenclature with Recommendations S17–S40 10. Chapter 4. Advisory Notes S41–S42 11. References S43–S44 12. Appendix 1. Codes of Nomenclature S45–S48 13. Appendix 2. Approved Lists of Bacterial Names S49–S49 14. Appendix 3. Published Sources for Names of Prokaryotic, Algal, Protozoal, Fungal, and Viral Taxa S50–S51 15. Appendix 4. Conserved and Rejected Names of Prokaryotic Taxa S52–S57 16. Appendix 5. Opinions Relating to the Nomenclature of Prokaryotes S58–S77 17. Appendix 6. Published Sources for Recommended Minimal Descriptions S78–S78 18. Appendix 7. Publication of a New Name S79–S80 19. Appendix 8. Preparation of a Request for an Opinion S81–S81 20. Appendix 9. Orthography S82–S89 21. Appendix 10. Infrasubspecific Subdivisions S90–S91 22. Appendix 11. The Provisional Status of Candidatus S92–S93 23.
    [Show full text]
  • The Waddlia Genome: a Window Into Chlamydial Biology
    The Waddlia Genome: A Window into Chlamydial Biology Claire Bertelli1., Franc¸ois Collyn1., Antony Croxatto1, Christian Ru¨ ckert2, Adam Polkinghorne3,4, Carole Kebbi-Beghdadi1, Alexander Goesmann2, Lloyd Vaughan4, Gilbert Greub1* 1 Center for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland, 2 Center for Biotechnology, Bielefeld University, Bielefeld, Germany, 3 Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia, 4 Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland Abstract Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first representative of the Waddliaceae family, W. chondrophila. The bacteria possesses a 291169312bp chromosome and a159593 bp low-copy number plasmid that might integrate into the bacterial chromosome. The Waddlia genome displays numerous repeated sequences indicating different genome dynamics from classical chlamydia which almost completely lack repetitive elements. Moreover, W. chondrophila exhibits many virulence factors also present in classical chlamydia, including a functional type III secretion system, but also a large complement of specific factors for resistance to host or environmental stresses. Large families of outer membrane proteins were identified indicating that these highly immunogenic proteins are not Chlamydiaceae specific and might have been present in their last common ancestor.
    [Show full text]
  • ESCCAR International Congress on Rickettsiae and Other Intracellular Bacteria Pierre-Edouard Fournier, Carole Kebbi-Beghdadi, Gilbert Greub
    ESCCAR international congress on Rickettsiae and other intracellular bacteria Pierre-Edouard Fournier, Carole Kebbi-Beghdadi, Gilbert Greub To cite this version: Pierre-Edouard Fournier, Carole Kebbi-Beghdadi, Gilbert Greub. ESCCAR international congress on Rickettsiae and other intracellular bacteria. Microbes and Infection, Elsevier, 2018, 20 (7-8, SI), pp.392-400. 10.1016/j.micinf.2018.08.001. hal-01935339 HAL Id: hal-01935339 https://hal.archives-ouvertes.fr/hal-01935339 Submitted on 11 Apr 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Microbes and Infection 20 (2018) 392e400 Contents lists available at ScienceDirect Microbes and Infection journal homepage: www.elsevier.com/locate/micinf Meeting report ESCCAR international congress on Rickettsiae and other intracellular bacteria * Pierre-Edouard Fournier a, Carole Kebbi-Beghdadi b, Gilbert Greub b, a URMITE, UniversitedelaMediterranee, Institut Hospitalo-Universitaire Mediterranee-Infection, Marseille, France b Institute of Microbiology, University of Lausanne and University Hospital, Faculty of Biology and Medicine, Lausanne, Switzerland article info Article history: broaden our knowledge and that technology-based research that Received 10 July 2018 allows to fish for new pathogens is also very important, despite Accepted 10 August 2018 being until recently not recognized by most funding agencies.
    [Show full text]
  • SHORT REPORT Role of Chlamydia Trachomatis and Emerging Chlamydia-Related Bacteria in Ectopic Pregnancy in Vietnam
    Epidemiol. Infect. (2015), 143, 2635–2638. © Cambridge University Press 2014 doi:10.1017/S0950268814003616 SHORT REPORT Role of Chlamydia trachomatis and emerging Chlamydia-related bacteria in ectopic pregnancy in Vietnam S. HORNUNG1,B.C.THUONG2,J.GYGER1, C. KEBBI-BEGHDADI3, 1 3,4 1 S. VASILEVSKY ,G.GREUB AND D. BAUD * 1 Materno-fetal and Obstetrics Research Unit, Department of Obstetrics and Gynaecology, Maternity, University Hospital, Lausanne, Switzerland 2 Tu Du Hospital, 284 Cong Quynh Pham Ngu Lao Ward, District 1, Ho Chi Minh City, Vietnam 3 Center for Research on Intracellular Bacteria, Institute of Microbiology, Faculty of Biology and Medicine, University of Lausanne and University Hospital, Lausanne, Switzerland 4 Infectious Disease Service, University Hospital, Lausanne, Switzerland Received 29 August 2014; Final revision 13 November 2014; Accepted 30 November 2014; first published online 29 December 2014 SUMMARY In this case-control study, we investigated the seroprevalence and molecular evidence of Chlamydia trachomatis and Waddlia chondrophila in ectopic pregnancies (EP) and uneventful control pregnancies in 343 women from Vietnam. Whereas presence of C. trachomatis IgG was strongly associated with EP [adjusted odds ratio (aOR) 5·41, 95% confidence interval (CI) 2·58–11·32], its DNA remained undetected in all tubal lesions. We confirmed an independent association between antibodies against Waddlia and previous miscarriage (aOR 1·87, 95% CI 1·02–3·42). Further investigations are needed to understand the clinical significance of Waddlia’s high seroprevalence (25·9% in control pregnancies) in this urban population. Key words: Adverse pregnancy outcome, Chlamydia-related bacteria, ectopic pregnancy, genital tract infection, intracellular bacteria.
    [Show full text]
  • W. Chondrophila: from Biology to Pathogenicity
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Serveur académique lausannois Serveur Académique Lausannois SERVAL serval.unil.ch Author Manuscript Faculty of Biology and Medicine Publication This paper has been peer-reviewed but dos not include the final publisher proof-corrections or journal pagination. Published in final edited form as: Title: Waddlia chondrophila: from biology to pathogenicity. Authors: de Barsy M, Greub G Journal: Microbes and infection / Institut Pasteur Year: 2013 Dec Volume: 15 Issue: 14-15 Pages: 1033-41 DOI: 10.1016/j.micinf.2013.09.010 In the absence of a copyright statement, users should assume that standard copyright protection applies, unless the article contains an explicit statement to the contrary. In case of doubt, contact the journal publisher to verify the copyright status of an article. 1 1 W. chondrophila: from biology to pathogenicity 2 Marie de Barsy1 and Gilbert Greub1 3 4 1 Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, 5 Switzerland 6 7 8 9 10 11 12 13 Corresponding author: 14 Prof. Gilbert Greub, MD PhD 15 Center for Research on Intracellular Bacteria (CRIB), Institute of Microbiology 16 University Hospital Center and University of Lausanne, 17 Bugnon 48, 1011 Lausanne, SWITZERLAND 18 Phone : +41 21 314 49 79, Fax : +41 21 341 40 60 19 e-mail : [email protected] 20 21 22 23 24 2 25 26 Abstract 27 Waddlia chondrophila is an emerging pathogen causing miscarriages in humans and abortions in 28 ruminants. The full genome of this Chlamydia-related bacterium has been recently completed, 29 providing new insights into its biology and evolution.
    [Show full text]
  • Chlamydiaceae Family, Parachlamydia Spp. and Waddlia Spp. in Porcine Abortion
    Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2012 Chlamydiaceae family, Parachlamydia spp., and Waddlia spp. in porcine abortion Koschwanez, M ; Meli, M ; Vögtlin, A ; Greub, G ; Sidler, X ; Handke, M ; Sydler, T ; Kaiser, C ; Pospischil, A ; Borel, N Abstract: At present, despite extensive laboratory investigations, most cases of porcine abortion remain without an etiological diagnosis. Due to a lack of recent data on the abortigenic effect of order Chlamydi- ales, 286 fetuses and their placentae of 113 abortion cases (1-5 fetuses per abortion case) were investigated by polymerase chain reaction (PCR) methods for family Chlamydiaceae and selected Chlamydia-like or- ganisms such as Parachlamydia acanthamoebae and Waddlia chondrophila. In 0.35% of the cases (1/286 fetuses), the Chlamydiaceae real-time PCR was positive. In the Chlamydiaceae-positive fetus, Chlamydia abortus was detected by a commercial microarray and 16S ribosomal RNA PCR followed by sequencing. The positive fetus had a Porcine circovirus-2 coinfection. By the Parachlamydia real-time PCR, 3.5% (10/286 fetuses of 9 abortion cases) were questionable positive (threshold cycle values: 35.0-45.0). In 2 of these 10 cases, a confirmation by Chlamydiales-specific real-time PCR was possible. All samples tested negative by the Waddlia real-time PCR. It seems unlikely that Chlamydiaceae, Parachlamydia, and Waddlia play an important role as abortigenic agents in Swiss sows. DOI: https://doi.org/10.1177/1040638712452729 Other titles: Short running title: Chlamydiales in porcine abortions Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-71468 Journal Article Accepted Version Originally published at: Koschwanez, M; Meli, M; Vögtlin, A; Greub, G; Sidler, X; Handke, M; Sydler, T; Kaiser, C; Pospischil, A; Borel, N (2012).
    [Show full text]
  • Novel Chlamydia Species Isolated from Snakes Are Temperature
    www.nature.com/scientificreports There are amendments to this paper OPEN Novel Chlamydia species isolated from snakes are temperature- sensitive and exhibit decreased Received: 17 July 2017 Accepted: 22 March 2018 susceptibility to azithromycin Published: xx xx xxxx Eveline Staub1, Hanna Marti1, Roberta Biondi2, Aurora Levi2, Manuela Donati2, Cory Ann Leonard1, Serej D. Ley1,5, Trestan Pillonel 3, Gilbert Greub 3, Helena M. B. Seth-Smith1,4,6 & Nicole Borel1 Chlamydia species have recently been recognized as emerging pathogens in snakes. However, isolation of novel snake chlamydiae is critical and their growth characteristics are largely unknown. In this study, two novel chlamydial species are described: Chlamydia serpentis and Chlamydia poikilothermis, isolated after attempts on 23 cloacal and choanal swabs from 18 PCR-positive captive snakes originating from diferent Swiss snake collections. Isolation success, growth curve and infectivity rates over a 48-hour time period were dependent on temperature (37 °C for C. serpentis, 28 °C for C. poikilothermis). C. serpentis and C. poikilothermis were sensitive to tetracycline and moxifoxacin during evaluation by in vitro antibiotic susceptibility assay but intermediate to resistant (2–4 μg/ml) to azithromycin. Whole genome sequencing of the isolates provided proof of the novel species status, and gives insights into the evolution of these branches of genus Chlamydia. Bacteria within the order Chlamydiales are biologically unique, obligate intracellular pathogens afecting humans, wild and domesticated mammals and reptiles, comprising to date nine families (reviewed in)1. Diseases and complications caused can include late abortions, infertility, trachoma and pneumonia. Chlamydiosis has been detected in both free-ranging and captive reptiles, causing granulomatous lesions in inner organs2–5, prolifera- tive pneumonia6,7, necrotizing enteritis or myocarditis8, hepatitis and conjunctivitis9,10 or remaining asympto- matic7,11,12.
    [Show full text]
  • A Zebrafish Model for Chlamydia Infection with the Obligate Intracellular Pathogen Waddlia Chondrophila
    Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2016 A zebrafish model for Chlamydia infection with the obligate intracellular pathogen Waddlia chondrophila Fehr, A ; Ruetten, M ; Seth-Smith, H ; Nufer, Lisbeth ; Voegtlin, A ; Lehner, Angelika ; Greub, Gilbert ; Crosier, P ; Neuhauss, S C F ; Vaughan, Lloyd Abstract: Obligate intracellular chlamydial bacteria of the Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) superphylum are important pathogens of terrestrial and marine vertebrates, yet many features of their pathogenesis and host specificity are still unknown. This is particularly true for families such as the Waddliacea which, in addition to epithelia, cellular targets for nearly all Chlamydia, can infect and replicate in macrophages, an important arm of the innate immune system or in their free-living amoebal counterparts. An ideal pathogen model system should include both host and pathogen, which led us to develop the first larval zebrafish model for chlamydial infections with Waddlia chondrophila. By varying the means and sites of application, epithelial cells of the swim bladder, endothelial cells of the vasculature and phagocytosing cells of the innate immune system became preferred targets for infection in zebrafish larvae. Through the use of transgenic zebrafish, we could observe recruitment of neutrophils to the infection site and demonstrate for the first time that W. chondrophila is taken up and replicates in these phagocytic cells and not only in macrophages. Furthermore, we present evidence that myeloid differentiation factor 88 (MyD88) mediated signaling plays a role in the innate immune reaction toW. chondrophila, eventually by Toll-like receptor (TLRs) recognition.
    [Show full text]