DOCSLIB.ORG

Comparative Genomic Analyses of Corynebacterium Pseudotuberculosis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

Comparative genomic analyses of Corynebacterium pseudotuberculosis Florence Elizabeth Pethick B.Sc. (Hons) A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy at the Faculty of Veterinary Medicine, University of Glasgow May 2013 © Florence Elizabeth Pethick 2013 To Mum and Dad with love and thanks... ...& to Grandpa, whom I know has been beside me through every page. ii Abstract This study set out to sequence the genome of Corynebacterium pseudotuberculosis (Cp) 3/99-5, an ovine strain isolated from a naturally-occurring case of caseous lymphadenitis (CLA) in Scotland. The isolate was sequenced and assembled by 454 Life Sciences, and then gap closure performed by ‘PCR bridging’. The resulting sequence consisted of three contigs with a length of 2,319,079 bp and a G+C content of 52.18%. The genome was then annotated and predicted to contain 2,153 coding sequences. Analysis of the coding sequences revealed the presence of several putative virulence factors, including four sortases with multiple sortase target proteins containing LPXTG motifs. A further two Cp strains, an Australian ovine and a North American equine isolate, as well as C. ulcerans NCTC 12077 were sequenced for comparison. Comparative genomics, both intra- and inter-species showed all the genomes to be highly homologous. However, the C. ulcerans genome is larger than the Cp genomes and is more distinct; it was found to be more similar to the equine Cp 1/06-A isolate which is the most diverged of the Cp isolates. Phylogenetic analyses of the Corynebacterium genus were performed using house-keeping loci but also secreted protein loci from Cp 3/99-5. Bayesian analysis of house-keeping loci distinguished the bacteria to a species level. Inclusion of secreted protein loci did not distinguish the isolates any further. The main objective of this work was to utilise the Cp genome sequence to identify potential diagnostic targets which could be used to augment the available ELITEST CLA or replace it. The ELITEST CLA is the only diagnostic test for CLA that exists on the commercial market in the UK. However, due to low specificity and sensitivity, it is only operated on a flock/group basis. Analyses of the Cp 3/99-5 genome identified several potential diagnostic candidates and seven protein targets were investigated further. Attempts were made to express these candidates as recombinant proteins, however, only two recombinants were successfully expressed and purified, Cp3995_0570 and CP40. The seroreactivity of these were then assessed by IgG ELISA using a panel of ten positive and ten negative CLA ovine sera. The sera were previously defined as positive or negative by PLD and whole cell ELISAs; both of which showed a significant difference between sera types. However, neither Cp3995_0570 nor CP40 distinguished between sera originating from Cp-infected and Cp-naïve animals. iii Declaration The work reported in this thesis was carried out under the supervision of Dr Michael C. Fontaine and Dr Alex F. Lainson at the Moredun Research Institute and Professor David G. E. Smith at the Moredun Research Institute and the Institute for Infection, Immunity & Inflammation, University of Glasgow. All results presented, unless otherwise stated, are the sole work of this author, as is the composition of this thesis. Signed: Date: iv Acknowledgments First and foremost I would like to thank my supervisors, Prof David G.E Smith, Dr Alex Lainson and Dr Mike Fontaine for their guidance throughout this project. In particular thanks go to Alex but also Raja Yaga and Dushyanth Jyothi for introducing me to the ever- expanding world of bioinformatics. I would like to thank Dr Frank Wright for phylogenetics assistance and for helpful and informative discussions. Thanks must also go to Quality Meat Scotland, The English Beef and Lamb Executive and Hybu Cig Cymru (Meat Promotion Wales) who funded this work. Importantly I would like to thank both past and present members of lab. 3.060, particularly Colin and Allen for their technical help, but also Sabrina, Anita, Tammy, Eleanor, Pam, Scott, Craig and Chris for making those long days in the lab much more enjoyable. Thanks also go to my fellow PhD students who were always a valuable source of encouragement and advice especially Sam, Heather and Cassie. More recently I would like to thank my colleagues at the University of Strathclyde for their endless support. Huge thanks especially go to Gillian, Mark, Richard and Vartul for their encouragement at a time when completion of the thesis seemed unreachable. I owe thanks to all the girls at Edinburgh Civil Service Ladies Hockey Club for their support, and helping me to vent when it was much needed. Heartfelt thanks must go to my friends and family, particularly my Mum and Dad, who have always been incredibly supportive and understanding, not to mention patient. Their numerous phone calls have helped through the troublesome stages and they have constantly seen the potential in me to achieve something which at times, I did not think I could, but which I am now proud of. Last but not least I would like to say a massive thank you to John for his support, understanding, love and kindness. But most of all, I thank him for keeping me calm amd strong and pushing me to stick with it when things got tough, coining the term ‘TUP’ in the process. v Contents Abstract ........................................................................................................................... iii Declaration ...................................................................................................................... iv Acknowledgments ............................................................................................................ v Contents .......................................................................................................................... vi List of Figures ................................................................................................................. ix List of Tables .................................................................................................................. xi Abbreviations................................................................................................................. xii 1. Chapter One: General Introduction ...................................................... 1 1.1 The genus Corynebacterium .................................................................................. 2 1.1.1 General characteristics of the genus Corynebacterium .................................................................. 2 1.1.2 Notable Corynebacteria .................................................................................................................. 3 1.2 Corynebacterium pseudotuberculosis .................................................................... 5 1.2.1 Phenotypic characterisation ............................................................................................................ 5 1.2.2 Virulence factors of C. pseudotuberculosis .................................................................................... 7 1.2.3 Diseases caused by Corynebacterium pseudotuberculosis ............................................................. 9 1.3 Caseous lymphadenitis ........................................................................................ 11 1.3.1 Introduction .................................................................................................................................. 11 1.3.2 Distribution and prevalence of CLA ............................................................................................ 11 1.3.3 Economic significance of CLA in sheep ...................................................................................... 12 1.3.4 Pathogenesis and transmission ..................................................................................................... 13 1.4 Treatment of CLA in sheep ................................................................................ 16 1.5 Vaccines against CLA ......................................................................................... 17 1.5.1 Bacterin vaccines .......................................................................................................................... 17 1.5.2 Toxoid vaccines ............................................................................................................................ 18 1.5.3 Combined vaccines ....................................................................................................................... 18 1.5.4 Live vaccines ................................................................................................................................ 19 1.5.5 DNA vaccines ............................................................................................................................... 20 1.6 Diagnosis of CLA ................................................................................................. 21 1.6.1 Conventional identification .......................................................................................................... 21 1.6.2 Analytical Profile Index (API) Identification ............................................................................... 22 1.6.3 Serological tests ............................................................................................................................ 23 1.7 Population biology ..............................................................................................
Recommended publications
  • ID 2 | Issue No: 4.1 | Issue Date: 29.10.14 | Page: 1 of 24 © Crown Copyright 2014 Identification of Corynebacterium Species

    ID 2 | Issue No: 4.1 | Issue Date: 29.10.14 | Page: 1 of 24 © Crown Copyright 2014 Identification of Corynebacterium Species

    UK Standards for Microbiology Investigations Identification of Corynebacterium species Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 2 | Issue no: 4.1 | Issue date: 29.10.14 | Page: 1 of 24 © Crown copyright 2014 Identification of Corynebacterium species Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: Standards Unit Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratories UK Standards for Microbiology Investigations are produced in association with: Logos correct at time of publishing. Bacteriology – Identification | ID 2 | Issue no: 4.1 | Issue date: 29.10.14 | Page: 2 of 24 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Corynebacterium species Contents ACKNOWLEDGMENTS .........................................................................................................
  • Attachment 2 a Risk Profile of Dairy Products in Australia Appendices 1

    Attachment 2 a Risk Profile of Dairy Products in Australia Appendices 1

    2-06 22 March 2006 Attachment 2 A Risk Profile of Dairy Products in Australia Appendices 1-6 DRAFT ASSESSMENT REPORT PROPOSAL P296 PRIMARY PRODUCTION AND PROCESSING STANDARD FOR DAIRY RISK PROFILE OF DAIRY PRODUCTS IN AUSTRALIA VII APPENDICES 1. IMPACT OF PROCESSING ON DAIRY PRODUCT SAFETY 1.1 Milk and cream 1.2 Cheese 1.3 Dried milk powders 1.4 Infant formulae 1.5 Concentrated milk products 1.6 Butter and butter products 1.7 Ice cream 1.8 Cultured and fermented milk products 1.9 Dairy desserts 1.10 Dairy based dips 1.11 Casein, why products and other functional milk derivatives 1.12 Colostrum 2. EPIDEMIOLOGICAL INFORMATION ON OUTBREAKS OF FOODBORNE ILLNESS ASSOCIATED WITH DAIRY PRODUCTS 3. OCCURRENCE OF MICROBIOLOGICAL HAZARDS ASSOCIATED WITH DAIRY PRODUCTS 4. CONSUMPTION FIGURES OF DAIRY PRODUCTS FOR AUSTRALIAN CONSUMERS 5. HAZARD IDENTIFICATION/HAZARD CHARACTERISATION OF PATHOGENS 5.1 Aeromonas Spp. 5.2 Bacillus cereus 5.3 Brucella Spp. 5.4 Campylobacter jejuni/coli 5.5 Clostridium Spp. 5.6 Coxiella burnetii 5.7 Corynebacterium ulcerans 5.8 Cryptosporidium 5.9 Enterobacter sakazakii 5.10 Pathogenic Escherichia coli 5.11 Listeria monocytogenes 5.12 Mycobacterium bovis 5.13 Mycobacterium avium subsp. paratuberculosis 5.14 Salmonella Spp. 5.15 Shigella Spp. 5.16 Staphylococcus aureus 5.17 Streptococcus Spp. 5.18 Yersinia enterocolitica 6. PREVIOUS RISK ASSESSMENTS ON MICROBIOLOGICAL PATHOGENS IN DAIRY PRODUCTS 7. CHEMICAL RISK ASSESSMENT FRAMEWORK 8. REGULATORY FRAMEWORK FOR AGRICULTURAL AND VETERINARY CHEMICALS 9. MAXIMUM RESIDUE LIMITS 10. CHEMICAL RESIDUES MEASURED IN BOVINE DAIRY PRODUCTS 11. REGISTERED ANTIMICROBIAL AGENTS 12.
  • Corynebacterium Species Rarely Cause Orthopedic Infections

    Corynebacterium Species Rarely Cause Orthopedic Infections

    Zurich Open Repository and Archive University of Zurich Main Library Strickhofstrasse 39 CH-8057 Zurich www.zora.uzh.ch Year: 2018 Corynebacterium species rarely cause orthopedic infections Kalt, Fabian ; Schulthess, Bettina ; Sidler, Fabian ; Herren, Sebastian ; Fucentese, Sandro F ; Zingg, Patrick O ; Berli, Martin ; Zinkernagel, Annelies S ; Zbinden, Reinhard ; Achermann, Yvonne Abstract: Corynebacterium spp. are rarely considered as pathogens but data in orthopedic infections are sparse. Therefore, we asked how often Corynebacterium spp. caused an infection in a defined cohort of orthopedic patients with a positive culture. In addition, we aimed to determine the species variety and susceptibility of isolated strains in regards to potential treatment strategies. Between 2006 and 2015, we retrospectively assessed all Corynebacterium sp. bone and joint cultures from an orthopedic ward. The isolates were considered as relevant indicating an infection if the same Corynebacterium sp. was present in at least two samples. We found 97 orthopedic cases with isolation of Corynebacterium spp. (128 positive samples), mainly Corynebacterium tuberculostearicum (n=26), Corynebacterium amycolatum (n=17), Corynebacterium striatum (n=13), and Corynebacterium afermentans (n=11). Compared to a cohort of positive blood cultures, we found significantly more C. striatum and C. tuberculostearicum but no C. jeikeium cases. Only 16 cases out 66 cases (24.2%) with an available diagnostic set of at least 2 samples had an infection. Antibiotic susceptibility testing (AST) of different antibiotics showed various susceptibility results except for vancomycin and linezolid with a 100% susceptibility rate. Rates of susceptibility of corynebacteria isolated from orthopedic samples and of isolates from blood cultures were comparable. In conclusion, our study results confirmed that Corynebacterium sp.
  • Corynebacterium Bovis: a Rare Case of Persistent Bacterial Keratitis and Corneal Perforation

    Corynebacterium Bovis: a Rare Case of Persistent Bacterial Keratitis and Corneal Perforation

    Open Access Case Report DOI: 10.7759/cureus.16913 Corynebacterium Bovis: A Rare Case of Persistent Bacterial Keratitis and Corneal Perforation Mohammed Elsheikh 1 , Ahmed Elsayed 1 , Nicholas Bennett 1 , Martin Connor 2 1. Ophthalmology, NHS Dumfries & Galloway, Dumfries, GBR 2. Microbiology, NHS Dumfries & Galloway, Dumfries, GBR Corresponding author: Mohammed Elsheikh, [email protected] Abstract We report a rare case of severe, non-contact lens-related Corynebacterium bovis corneal infection on a background of viral keratitis, resulting in corneal abscess formation with subsequent corneal perforation. An 89-year-old Caucasian lady presented with a significant epithelial defect and a dense stromal infiltrates on a background of herpes zoster keratitis, ultimately resulting in corneal perforation. Enrichment culture obtained from corneal scraping isolated the unusual organism Corynebacterium bovis. This was treated with a combination of culture-directed, targeted course of antibiotics and surgical interventions. To the best of our knowledge, this is the first reported case of profuse bacterial keratitis secondary to Corynebacterium bovis infiltration, on a background of viral keratitis, resulting in corneal abscess formation and subsequent perforation. This report highlights this rare bacterium’s characteristics including its pathogenicity in causing severe corneal disease, particularly in immunosuppressed environments such as in this case, apparent antibiotic sensitivities & resistance, and potential transmission route. Categories: Ophthalmology, Infectious Disease Keywords: corynebacterium bovis, cornea, microbial keratitis, corneal perforation, corneal infection Introduction Corynebacterium species are frequently isolated on the conjunctiva of healthy adults and are thus increasingly regarded as non-pathogenic bacteria [1,2]. Recently, however, there have been increasing reports detailing the pathogenicity of Corynebacterium species isolated on ocular surfaces, with immunosuppression representing a risk factor [3-6].
  • NGS-Based Phylogeny of Diphtheria-Related Pathogenicity Factors in Different Corynebacterium Spp

    NGS-Based Phylogeny of Diphtheria-Related Pathogenicity Factors in Different Corynebacterium Spp

    Dangel et al. BMC Microbiology (2019) 19:28 https://doi.org/10.1186/s12866-019-1402-1 RESEARCHARTICLE Open Access NGS-based phylogeny of diphtheria-related pathogenicity factors in different Corynebacterium spp. implies species- specific virulence transmission Alexandra Dangel1* , Anja Berger1,2*, Regina Konrad1 and Andreas Sing1,2 Abstract Background: Diphtheria toxin (DT) is produced by toxigenic strains of the human pathogen Corynebacterium diphtheriae as well as zoonotic C. ulcerans and C. pseudotuberculosis. Toxigenic strains may cause severe respiratory diphtheria, myocarditis, neurological damage or cutaneous diphtheria. The DT encoding tox gene is located in a mobile genomic region and tox variability between C. diphtheriae and C. ulcerans has been postulated based on sequences of a few isolates. In contrast, species-specific sequence analysis of the diphtheria toxin repressor gene (dtxR), occurring both in toxigenic and non-toxigenic Corynebacterium species, has not been done yet. We used whole genome sequencing data from 91 toxigenic and 46 non-toxigenic isolates of different pathogenic Corynebacterium species of animal or human origin to elucidate differences in extracted DT, DtxR and tox-surrounding genetic elements by a phylogenetic analysis in a large sample set. Results: Sequences of both DT and DtxR, extracted from whole genome sequencing data, could be classified in four distinct, nearly species-specific clades, corresponding to C. diphtheriae, C. pseudotuberculosis, C. ulcerans and atypical C. ulcerans from a non-toxigenic toxin gene-bearing wildlife cluster. Average amino acid similarities were above 99% for DT and DtxR within the four groups, but lower between them. For DT, subgroups below species level could be identified, correlating with different tox-comprising mobile genetic elements.
  • ( 12 ) United States Patent

    ( 12 ) United States Patent

    US009956282B2 (12 ) United States Patent ( 10 ) Patent No. : US 9 ,956 , 282 B2 Cook et al. (45 ) Date of Patent: May 1 , 2018 ( 54 ) BACTERIAL COMPOSITIONS AND (58 ) Field of Classification Search METHODS OF USE THEREOF FOR None TREATMENT OF IMMUNE SYSTEM See application file for complete search history . DISORDERS ( 56 ) References Cited (71 ) Applicant : Seres Therapeutics , Inc. , Cambridge , U . S . PATENT DOCUMENTS MA (US ) 3 ,009 , 864 A 11 / 1961 Gordon - Aldterton et al . 3 , 228 , 838 A 1 / 1966 Rinfret (72 ) Inventors : David N . Cook , Brooklyn , NY (US ) ; 3 ,608 ,030 A 11/ 1971 Grant David Arthur Berry , Brookline, MA 4 ,077 , 227 A 3 / 1978 Larson 4 ,205 , 132 A 5 / 1980 Sandine (US ) ; Geoffrey von Maltzahn , Boston , 4 ,655 , 047 A 4 / 1987 Temple MA (US ) ; Matthew R . Henn , 4 ,689 ,226 A 8 / 1987 Nurmi Somerville , MA (US ) ; Han Zhang , 4 ,839 , 281 A 6 / 1989 Gorbach et al. Oakton , VA (US ); Brian Goodman , 5 , 196 , 205 A 3 / 1993 Borody 5 , 425 , 951 A 6 / 1995 Goodrich Boston , MA (US ) 5 ,436 , 002 A 7 / 1995 Payne 5 ,443 , 826 A 8 / 1995 Borody ( 73 ) Assignee : Seres Therapeutics , Inc. , Cambridge , 5 ,599 ,795 A 2 / 1997 McCann 5 . 648 , 206 A 7 / 1997 Goodrich MA (US ) 5 , 951 , 977 A 9 / 1999 Nisbet et al. 5 , 965 , 128 A 10 / 1999 Doyle et al. ( * ) Notice : Subject to any disclaimer , the term of this 6 ,589 , 771 B1 7 /2003 Marshall patent is extended or adjusted under 35 6 , 645 , 530 B1 . 11 /2003 Borody U .
  • Cell Envelope of Corynebacteria: Structure and Influence on Pathogenicity

    Cell Envelope of Corynebacteria: Structure and Influence on Pathogenicity

    Hindawi Publishing Corporation ISRN Microbiology Volume 2013, Article ID 935736, 11 pages http://dx.doi.org/10.1155/2013/935736 Review Article Cell Envelope of Corynebacteria: Structure and Influence on Pathogenicity Andreas Burkovski Lehrstuhl fur¨ Mikrobiologie, Friedrich-Alexander-Universitat¨ Erlangen-Nurnberg,¨ Staudtstre 5, 91058 Erlangen, Germany Correspondence should be addressed to Andreas Burkovski; [email protected] Received 2 December 2012; Accepted 31 December 2012 Academic Editors: S. H. Flint, G. Koraimann, and T. Krishnan Copyright © 2013 Andreas Burkovski. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To date the genus Corynebacterium comprises 88 species. More than half of these are connected to human and animal infections, with the most prominent member of the pathogenic species being Corynebacterium diphtheriae, which is also the type species of the genus. Corynebacterium species are characterized by a complex cell wall architecture: the plasma membrane of these bacteria is followed by a peptidoglycan layer, which itself is covalently linked to a polymer of arabinogalactan. Bound to this, an outer layer of mycolic acids is found which is functionally equivalent to the outer membrane of Gram-negative bacteria. As final layer, free polysaccharides, glycolipids, and proteins are found. The composition of the different substructures of the corynebacterial cell envelope and their influence on pathogenicity are discussed in this paper. 1. The Genus Corynebacterium Based on their medical importance as etiological agent of diphtheria and their enormous biotechnological potential, The genus Corynebacterium belongs to the class of Acti- C.
  • Septicaemiain a Granulocytopenic Patientcaused By

    Septicaemiain a Granulocytopenic Patientcaused By

    - Postgraduate Medical Journal (1989) 65, 247 248 Postgrad Med J: first published as 10.1136/pgmj.65.762.247 on 1 April 1989. Downloaded from Septicaemia in a granulocytopenic patient caused by Corynebacterium striatum Lawrence Dall', William G. Barnes2 and Dennis Hurford' 'Department ofMedicine, University ofMissouri, School ofMedicine-Kansas City, 2411 Holmes Street, Kansas City, Missouri 64108 and2Department ofPathology, Truman Medical Center, and School ofMedicine, University ofMissouri-Kansas City, 2301 Holmes Street, Kansas City, Missouri 64108, USA. Summary: A 64 year old woman with metastatic endometrial carcinoma was admitted to the hospital after three grand mal seizures. Blood cultures yielded Corynebacterium striatum. The patient responded to parenteral ampicillin therapy. This is believed to be the first case of sepsis caused by this organism. Introduction Neutropenic patients are susceptible to a wide variety platelet count 95 x 109/1, and white blood cell count of infections from unusual organisms. The incidence 0.99 x 109/l (48% polymorphonuclear leucocytes). of bacteraemia increases as the neutrophil count falls Chest X-ray, and cranial computed tomography below 500 x 106/1 with most of the organisms revealed multiple metastatic lesions. Two sets ofblood originating from the patient's endogenous flora. The cultures (4/4 bottles) obtained on admission revealed a organism implicated in this case, Corynebacterium nonsporulating, aerobic, weakly haemolytic, non- striatum, is recognized as a part of the microbial flora motile, catalase positive, pleomorphic diptheroid-like, of the human skin and nasal mucous membranes. Gram-positive bacillus which fermented glucose, fruc- We believe this report to be the first documented tose, mannose, lactose and maltose.
  • Characterization of Corynebacterium Species in Macaques

    Characterization of Corynebacterium Species in Macaques

    Journal of Medical Microbiology (2012), 61, 1401–1408 DOI 10.1099/jmm.0.045377-0 Characterization of Corynebacterium species in macaques Jaime Venezia,1 Pamela K. Cassiday,2 Robert P. Marini,1 Zeli Shen,1 Ellen M. Buckley,1 Yaicha Peters,1 Nancy Taylor,1 Floyd E. Dewhirst,3,4 Maria L. Tondella2 and James G. Fox1 Correspondence 1Division of Comparative Medicine, Massachusetts Institute of Technology, James G. Fox 77 Massachusetts Avenue, Cambridge, MA 02139, USA [email protected] 2Division of Bacterial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA 3Department of Molecular Genetics, The Forsyth Institute, 245 First Street, Cambridge, MA 02142, USA 4Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA 02115, USA Bacteria of the genus Corynebacterium are important primary and opportunistic pathogens. Many are zoonotic agents. In this report, phenotypic (API Coryne analysis), genetic (rpoB and 16S rRNA gene sequencing), and physical methods (MS) were used to distinguish the closely related diphtheroid species Corynebacterium ulcerans and Corynebacterium pseudotuberculosis, and to definitively diagnose Corynebacterium renale from cephalic implants of rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques used in cognitive neuroscience research. Throat and cephalic implant cultures yielded 85 isolates from 43 macaques. Identification by API Coryne yielded C. ulcerans (n574), Corynebacterium pseudotuberculosis (n52), C. renale or most closely related to C. renale (n53), and commensals and opportunists (n56). The two isolates identified as C. pseudotuberculosis by API Coryne required genetic and MS analysis for accurate characterization as C. ulcerans. Of three isolates identified as C.
  • Detection of Corynebacterium Bovis Infection in Athymic Nude Mice from a Research Animal Facility in Korea

    Detection of Corynebacterium Bovis Infection in Athymic Nude Mice from a Research Animal Facility in Korea

    pISSN 1229-845X, eISSN 1976-555X JOURNAL OF J. Vet. Sci. (2014), 15(4), 583-586 http://dx.doi.org/10.4142/jvs.2014.15.4.583 Veterinary Received: 5 Feb. 2014, Revised: 8 May 2014, Accepted: 19 Jun. 2014 Science Case Report Detection of Corynebacterium bovis infection in athymic nude mice from a research animal facility in Korea Tae-Hyoun Kim1,2, Dong-Su Kim1, Ju-Hee Han1,2, Seo-Na Chang1,2, Kyung-Sul Kim1, Seung-Hyeok Seok3, Dong-Jae Kim4, Jong-Hwan Park4, Jae-Hak Park1,2,* 1Department of Laboratory Animal Medicine, and 2BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea 3Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 110-799, Korea 4Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-832, Korea Corynebacterium (C.) bovis infection in nude mice causes examination, Test mouse 1 did not present any clinical hyperkeratosis and weight loss and has been reported signs. In contrast, Test mouse 2 and Test mouse 3 had worldwide but not in Korea. In 2011, nude mice from an yellow-white scales on the dorsal skin (Fig. 1), and their animal facility in Korea were found to have white flakes on weight was lower than that suggested by the vendor (Table their dorsal skin. Histopathological testing revealed that the 1). For further diagnosis, matched healthy control mice mice had hyperkeratosis and Gram-positive bacteria were (Central Lab Animal, Korea) were purchased and included found in the skin.
  • Corynebacterium Rouxii Sp. Nov., a Novel Member of the Diphtheriae

    Corynebacterium Rouxii Sp. Nov., a Novel Member of the Diphtheriae

    Corynebacterium rouxii sp. nov., a novel member of the diphtheriae species complex Edgar Badell, Melanie Hennart, Carla Rodrigues, Virginie Passet, Mélody Dazas, Valerie Bouchez, Leonardo Panunzi, Annick Carmi-Leroy, Julie Toubiana, Sylvain Brisse To cite this version: Edgar Badell, Melanie Hennart, Carla Rodrigues, Virginie Passet, Mélody Dazas, et al.. Corynebac- terium rouxii sp. nov., a novel member of the diphtheriae species complex. Research in Microbiology, Elsevier, 2020, 171 (3-4), pp.122-127. 10.1016/j.resmic.2020.02.003. pasteur-02862963v2 HAL Id: pasteur-02862963 https://hal-pasteur.archives-ouvertes.fr/pasteur-02862963v2 Submitted on 18 Jun 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives| 4.0 International License Research in Microbiology 171 (2020) 122e127 Contents lists available at ScienceDirect Research in Microbiology journal homepage: www.elsevier.com/locate/resmic Original Article Corynebacterium rouxii sp. nov., a novel member of the diphtheriae
  • Antibiotic Susceptibility of Major Bacteria Cause Ovine Mastitis in River Nile State, Sudan

    Antibiotic Susceptibility of Major Bacteria Cause Ovine Mastitis in River Nile State, Sudan

    Imperial Journal of Interdisciplinary Research (IJIR) Vol-3, Issue-1, 2017 ISSN: 2454-1362, http://www.onlinejournal.in Antibiotic Susceptibility of Major Bacteria Cause Ovine Mastitis in River Nile State, Sudan Fadlalla E.2 ,1٭Abubaker A. El Ayis 1Department of Medicine, College of Veterinary Medicine, Bahri University (Sudan). 2Department of Biochemistry, College of Veterinary Medicine, Bahri University (Sudan). Abstract: In this study a total of 40 milk samples Procaine Penicillin and Cloxacillin. 50.0% of positive for California Mastitis Test (CMT) were Klebsiella pneumoniae isolates were sensitive to collected from ewes in the three localities of River Ciprofloxacin, Gentamycin and Kanamycin. All Nile State (Atbara, Barbar and El Damer). Samples strains were resistant to Ampicillin, Cephalothin, were submitted for bacteriological examination. Chloramphenicol, Erythromycin Procaine The aerobic bacteria isolated and identified from Penicillin, Cloxacillin and Tetracycline. All ewes’ milk samples were 19 Staphylococci Enterobacter spp. Isolates were sensetive to (21.0%), 9 Streptococci (9.9%), 5 Echerichia coli Cephalothin and Chloramphenicol, 66.7% to (5.6%), 4 Corynebacterium bovis (3.3%), 3 Ciprofloxacin, Tetracycline and Gentamycin and Enterobacter spp. (3.3%), 2 Klebsiella pneumoniae all isolates were resstant to Ampicillin, spp, (2.2%) and 2 Enterococcus faecalis (2.2%). Erythromycin, Kanamycin, Procaine Penicillin and All isolates were subjected to sensitivity tests using Cloxacillin. 10 antibiotics in use for treatment of mastitis in Sudan. 89.5% Staphylococcal isolates were I. INTRODUCTION sensitive to Ampicillin, 73.3% to Cephalothin, 68.4% to Ciprofloxacin and Kanamycin, 63.2% to Mastitis is one of the more common health Gentamycin, 57.9% to Chloramphenicol, 26.3% to problems affecting sheep and goats.