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13 http://serversmiso.aecs.sy/ lLa1 "k. A 4jjHI tilkll Greany, P. D. and J. E. Capenter, 2000. Use of nuclear techniques in biological control. In Area-Wide Control of Fruit Flies and other Pests, ed. K. H. Tan. Pennang, Malaysia. Penerbit Universiti sains Malaysia, PP. 221-227. Hallman G. 2000. Expanding radiation quarantine treatments beyond fruit flies. Agr. and Forest Entomology. 2:85-95. Hendrichs J., K. Bloem, G. Hoch, J. E. Carpenter, P. Greany and A. S. Robinson. 2009. Improving the cost effectiveness, trade and safty of biological control insect pests using nuclear techniques. Biocontrol Sci. and Tech. 19: 3-22. Keever D.W., M.A. Mullen, J. W. Press and R. T. Arbogast 1986. Augmentation of natural enemies for suppressing two major insect pests in stored farmers stock peanuts. Environ. Entomol. 15: 767-777 Makee H. and G. Saour 2003. Noninhirited sterility in irradiated Phthorimaea operculella females. J. Appl. Entomol. 127: 489-493. Mansour, M. Y., 2003. Gamma irradiation as a quarantine treatment for apples infested by codling moth (Lepidoptera: Tortricidae). J. Applied Entomology. 127: 137-141. Mansour, M. Y., 2010. Effects of gamma radiation on the Mediterranean flour moth, Ephestia kuehniella, eggs and acceptability of irradiated eggs by Trichogramma cacoeciae females. J. of Pest Science. 83: 243-249 Matthew J. G., P. W. Flinn and J. R. Nechols. 2006. Biological control of Indian meal moth (Lepidoptera: Pyralidae) on finished stored products using egg and larval parasi toids. J. Econ. Entomol. 99: 1080-1084. Phillips, T.W., and J. E. Throne. 2010. Biological approach to managing stored product unsects. Annu. Rev. of Entomol. 55: 375-397. Press JW, L. D. Cline and B. R. Flaherty 1982. A comparison of two , Bracon hebetor (Hymenoptera: Braconidae) and Ventiiria canescens (Hymenoptera: ), and a predator Xylocoris flavipes (Hemiptera: Anthocoridae) in suppressing residual populations of the , Ephestiacautella (Lepidoptera: Pyralidae). J. Kansas Entomol. Soc. 55: 125-128. Richard M., and W. S. Thompson. 1932. A contribution to the study of the genera Ephestia and Plodia (Lepidoptera, Phycitidae), with notes on parasites of the larvae. Trans. Ent. Soc. London 79: 439-492. Saour G. 2004. Parasitization of potato tuber moth eggs (Lep., Gelechiidae) from irradiated adults by Trichogramma (Hym., Trichogrammatidae) and control of moth

14 h tt p ://se rve rs m i so . a e cs. sy/ I * iM i uS A Ajjjj-iJl 451 fill 4jjjh ) £ population with combined releases of sterile insect and egg parasitoid. J. of Appl. Entomol.. 128: 681-686. Scholler M. and P. W. Flinn. 2000. Parasitoids and predators, in Alternatives to in Stored-Product IPM. Subramanyam B, Hagstrum DW, eds. Boston: Kluwer Academic, pp. 229-71 Scholler M„ P. W. Flinn, M. J. Grieshop and E. Z d a rkova . 2006. Biological control of stored-product pests. In Insect Management for Food Storage and Processing. Heaps JW, ed. St. Paul: AACC Int. 2nd ed., pp. 67-87 Scholler M., and S. Prozell, 2001. Die Mehlmottenschlupfwespe (Hymenoptera: Braconidae) als Antagonist vorratsscha"ditcher Molten. Gesunde Pflanz 53:82-89 Yu, S.H., M. Ryoo and J. H. Na 1999. Life history of Bracon hebetor (Say) (Hymenoptera: Braconidae) on Indian meal moth Plodia interpunctella (Lepidoptera: pyralodae) on dried vegetables commodity. J. Asia-Pacific Entomology. 2: 149-152. Zubrik, M. and J. Novotny 2009. Impact of gamma radiation on the developmental characteristics of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae) preparatory to their use as supplemental host/prey for natural enemy enhancement. Biocontrol Sci. and Tech. 19: 291-301.

15 http://serversmiso.aecs.sy/ LiLti1 "S A 4jjHI tilkll Abstract

Mediterranean flour moth, Ephestia knehniella, mature 5th instar larvae were exposed to gamma radiation dosages ranging from 50-350 Gy at 50 Gy increment and the effects of gamma radiation on pupation, adult emergence and sex ratio were investigated. In addition, effects of irradiation on the rate of development to pupae and adults and the acceptance of irradiated larvae by B. hebetor females were examined. Results showed that the radio-sensitivity of E. kuehmella larvae increased with increasing dose. Pupation was significantly affected at 100 Gy dose, and completely prevented at 350 Gy dose. Adult emergence was only slightly different from pupation; emergence was significantly affected at 100 Gy dose and completely prevented at 300 Gy dose. Irradiation also negatively affected the rate of development of larvae to pupae and adults. While more than 98% of control larvae pupated within 10 days of treatment, this ratio decreased to about 79% in larvae exposed to 50 Gy and less than 4% in larvae exposed to 100 Gy dose. The rate of development to the adult stage was also similarly affected. While 97% of control insects emerged as adults in the 1st 20 days of treatment, this ratio decreased to about 53, 2 and zero percent in larvae exposed to 50, 100 and 150 Gy dose respectively. In addition, irradiation significantly affected male: female ratio; sex ratio was skewed in favor of males, and at 250 Gy dose all emerged were males. Furthermore, irradiation had no adverse effect on the degree of acceptance of mature larvae to parasitization; irradiated insects were as acceptable to B. hebetor females as unirradiated ones.

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17 http://serversmiso.aecs.sy/ c^Ujk^i ^ AJlJall 4jjA w SYRIAN ARAB REBABLIC ATOMIC ENERGY COMMISSION DAMASCUS- P.O.BOX: 6091

Report on laboratory Reconnaissance Experiment Department of Agriculture

Effects of gamma radiation on the Mediterranean flour moth, Ephestia kuehniella, mature larvae and acceptability of irradiated larvae by Bracon hebetor females

Dr. M. Mansour

AECS - AYRRE 224 June 2011

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